Wiley Acta Medica Okayama 1434-193X 2026 Informatics‐Driven and Automated Optimization in Flow Electrochemical Synthesis e202501237 EN Eisuke Sato Department of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Akine Tani Department of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Tomohiro Nakahama Department of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Koichi Mitsudo Department of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Seiji Suga Department of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Electrochemical synthesis has emerged as a powerful platform for environmentally sustainable chemical transformations. When integrated with flow chemistry, electrosynthetic processes exhibit enhanced scalability, making them suitable for industrial applications. Recently, the integration of electrochemical flow systems with informatics techniques has accelerated the optimization of reaction conditions. Data-driven strategies facilitate rapid exploration of multidimensional parameter spaces, enabling identification of optimal reaction conditions with high efficiency. These advances have enabled the development of automated optimization systems. This review highlights recent progress in combining electrosynthesis, flow chemistry, and computational tools, focusing on representative examples that illustrate efficient optimization protocols and autonomous reaction development. By showcasing these developments, we discuss how the integration of these technologies is driving innovation in electrochemical synthesis. No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 2238-7854 42 2026 An electric field temporarily strengthens zirconia ceramics 1806 1810 EN Akira Kishimoto Faculty of Environmental, Life, Natural Science and Technology, Okayama University Takahiro Shimizu Faculty of Environmental, Life, Natural Science and Technology, Okayama University Mitsuru Nishiyama Faculty of Environmental, Life, Natural Science and Technology, Okayama University Shinya Kondo Faculty of Environmental, Life, Natural Science and Technology, Okayama University Takashi Teranishi Faculty of Environmental, Life, Natural Science and Technology, Okayama University By applying an electric field to yttria-stabilized zirconia (8YSZ) equipped with an inert electrode, oxide ions are localized near the positive electrode, causing it to expand. When polarization was performed under different conditions, it was possible to strengthen the material to 1.5 times that of an untreated sample. The lattice constant of the positive electrode surface after polarization was larger than before polarization. When the Vickers hardness of the positive electrode surface was measured by changing the test load, the smaller the load, the higher the hardness value. Polarization caused oxide ions to move near the positive electrode, filling in the defects and generating an expanded layer with a large lattice constant. It is believed that this was subjected to compressive stress from the bulk layer, which had not changed in volume, resulting in an increase in strength. No potential conflict of interest relevant to this article was reported.

岡山大学教育推進機構 教師教育開発センター Acta Medica Okayama 2186-1323 16 2026 立憲主義に対する認識の改善を目指した高等学校公民科の授業開発研究 ―米国公民教育センター開発『我ら合衆国人民』を手がかりにして― 167 180 EN Toshinori KUWABARA Faculty of Education, Okayama University Ayuha MIYAMOTO Graduate School of Humanities and Social Sciences, Okayama University 10.18926/CTED/70367 　本研究は、憲法理解の基本としての立憲主義に対する認識形成を目標とする、高等学校公民科で実践可能な授業の開発を目指したものである。従来の日本の社会科教育においては、憲法理解は日本国憲法の基本原則である国民主権、基本的人権の尊重、平和主義の理解を基本としていたが、近年、そもそも憲法とは何かを理解させるために、立憲主義の概念が注目されるようになり、教科書にも記述されている。本研究は、そのような立憲主義という概念について生徒に適切に理解させることを目指した授業の提案をしようとするものである。授業計画作成にあたっては、米国の公民教育センターが開発し、長年活用されている『我ら合衆国人民（原題 We the People）』を参照し、その一部を活用し、米国の歴史的背景に基づいて作られた教材を、日本の文脈にそって改変した。 No potential conflict of interest relevant to this article was reported.

岡山大学教育推進機構 教師教育開発センター Acta Medica Okayama 2186-1323 16 2026 刑法の意義を捉えさせる社会科における法教育実践の方法 ―模擬裁判を取り入れた中学校社会科の授業開発を通して― 153 165 EN Ayuha MIYAMOTO Graduate School of Human ities and So cial Sciences, Okayama University Toshinori KUWABARA Faculty of Education, Okayama University 10.18926/CTED/70366 　本研究は、刑法の理解に焦点をあて、模擬裁判を取り入れた中学校社会科の授業開発を行なおうとするものである。日本の社会科における法教育は、従来から憲法学習が中心となっており、その他の法律について学ぶ機会は非常に少ない。そのような現状を踏まえて、近年、民法や刑法などを取り上げた法教育の授業開発が行われるようになった。その一方で、裁判員制度導入以降、模擬裁判を取り入れた社会科授業の開発・実践がよく見られるようになっており、刑事事件が社会科授業で取り上げられることも珍しくはなくなった。しかし、そのような授業を担当する教員に、刑法等に関する知識が十分ではなく、模擬裁判の内容と実際の裁判が乖離しているという課題もある。本研究では、従来の模擬裁判を取り入れた授業の特質と課題を検討したうえで、刑法の意義を捉えさせる中学校社会科の授業開発を目指す。 No potential conflict of interest relevant to this article was reported.

Institute of Electrical and Electronics Engineers (IEEE) Acta Medica Okayama 2169-3536 14 2026 FEM-Based Design and Characterization of a Millimeter-Scale Piezoelectric Resonance Force Sensor 17960 17970 EN Aoto Yamazaki Department of Mechanical Engineering, Toyohashi University of Technology Takuma Akiduki Department of Mechanical Engineering, Toyohashi University of Technology Atsuo Honna Riccoh Company Ltd. Michiteru Kitazaki Department of Computer Science and Engineering, Toyohashi University of Technology Tomoaki Mashimo Graduate School of Natural Science and Technology, Okayama University This paper presents a millimeter-scale piezoelectric effect-based force sensor that uses the change in its resonant frequency as the detection principle for high sensitivity and a wide measurement range. Such characteristics are suited for robot hand applications that not only detect small forces but also handle large payloads. We develop a methodology to estimate the relationship between applied force and resonant frequency shift by combining classical contact theory and finite element method (FEM) analysis. Although this relationship is non-linear, the designability of sensitivity and measurement range is demonstrated by the simulation. The simulation results based on the method are verified, showing good agreement with the experimental results. The static characteristics, including sensitivity, standard deviation, and resolution, are evaluated using prototype sensors with characteristic lengths ranging from 1 mm to 4 mm. The 4-mm model has a measurement range of 77 mN to 300 N, and the smallest model, which is one of the smallest force sensors suitable for practical implementation, has a measurement range of 9 mN to 20 N. No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 1349-0079 68 1 2026 Insights into the taste of organic acids via TAS1Rs 100731 EN Yuko Yamase Department of Dental Anesthesiology and Special Care Dentistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Katsuki Takebe Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kengo Horie Department of Oral Physiology, Graduate School of Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Yoshihiro Mitoh Department of Oral Physiology, Graduate School of Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Atsuko Yamashita Institute for Protein Research, The University of Osaka Ryusuke Yoshida Department of Oral Physiology, Graduate School of Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Objectives: Organic acids contribute significantly to the flavor of fermented foods by imparting sourness. Although mice generally avoid sour taste, previous studies have reported greater consumption of l-lactic acid than its d-enantiomer, suggesting enantiomer-specific recognition. This behavior is hypothesized to involve TAS1Rs, which consists of sweet/umami receptors. However, it remains unclear whether TAS1Rs additionally contribute to the recognition of other chiral organic acids. This study aimed to evaluate the role of TAS1Rs, particularly TAS1R3, in the modulation of enantiomer-dependent behavioral responses to organic acids in mice.<br> Methods: Behavioral responses were evaluated using 48-h and 1-h 2-bottle tests. Binding of organic acids to TAS1Rs was investigated by differential scanning fluorimetry (DSF) with the ligand-binding domain (LBD) of medaka Tas1r2a/Tas1r3.<br> Results: Wild-type mice consumed more d-malic acid than l-malic acid in the 48-h test, whereas Tas1r3-KO mice showed no such difference. This pattern was not observed in the short-term 1-h test, which minimized the contribution of post-ingestion and learned effects. DSF analysis revealed no binding of any of the tested organic acids to the LBD of medaka Tas1r2a/Tas1r3.<br> Conclusions: Organic acids may elicit TAS1R3-dependent post-ingestion signals that contribute to enantiomer-selective consumption in mice. Electrostatic interactions and hydrogen-bonding networks within the orthosteric pocket of TAS1Rs may account for the differences in binding affinity to the LBD of medaka Tas1r2a/Tas1r3 between organic acids and L-alanine, a known ligand. No potential conflict of interest relevant to this article was reported.

American Association for the Advancement of Science (AAAS) Acta Medica Okayama 2375-2548 11 44 2025 Structural insights into the divergent evolution of a photosystem I supercomplex in Euglena gracilis eaea6241 EN Koji Kato Research Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Yoshiki Nakajima Research Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Runa Sakamoto Research Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Minoru Kumazawa Institute of Low Temperature Science, Hokkaido University Kentaro Ifuku Graduate School of Agriculture, Kyoto University Takahiro Ishikawa Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University Jian-Ren Shen Research Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Atsushi Takabayashi Institute of Low Temperature Science, Hokkaido University Ryo Nagao Faculty of Agriculture, Shizuoka University Photosystem I (PSI) forms supercomplexes with light-harvesting complexes (LHCs) to perform oxygenic photosynthesis. Here, we report a 2.82-angstrom cryo–electron microscopy structure of the PSI-LHCI supercomplex from Euglena gracilis, a eukaryotic alga with secondary green alga-derived plastids. The structure reveals a PSI monomer core with eight subunits and 13 asymmetrically arranged LHCI proteins. Euglena LHCIs bind diadinoxanthin, which is one of the carotenoids typically associated with red-lineage LHCs and is not present in the canonical LHCI belt found in green-lineage PSI-LHCI structures. Phylogenetic analysis shows that the Euglena LHCIs originated from LHCII-related clades rather than from the green-lineage LHCI group and that the nuclear-encoded PSI subunit PsaD likely originated from cyanobacteria via horizontal gene transfer. These observations indicate a mosaic origin of the Euglena PSI-LHCI. Our findings uncover a noncanonical light-harvesting architecture and highlight the structural and evolutionary plasticity of photosynthetic systems, illustrating how endosymbiotic acquisition and lineage-specific adaptation shape divergent light-harvesting strategies. No potential conflict of interest relevant to this article was reported.

Oxford University Press (OUP) Acta Medica Okayama 1083-7159 30 7 2025 Pharmacovigilance study for the identification of mogamulizumab-induced immune-related adverse events using a real-world database oyaf201 EN Koji Miyata Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Yuki Izawa-Ishizawa Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Takahiro Niimura Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Toshihiko Yoshioka Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Mizusa Hyodo Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Shuto Itokazu Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Tatsumi Miyata Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Fuka Aizawa Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Kenta Yagi Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Kei Kawada Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Hirofumi Hamano Department of Pharmacy, Okayama University Hospital Yoshito Zamami Department of Pharmacy, Okayama University Hospital Mitsuhiro Goda Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Keisuke Ishizawa Department of Clinical Pharmacology and Therapeutics, Graduate School of Biomedical Sciences, Tokushima University Background: Mogamulizumab is a humanized anti-CCR4 monoclonal antibody used for relapsed/refractory adult T-cell leukemia, cutaneous T-cell lymphoma, and/or Sézary syndrome. Reports of immune-related adverse events (irAEs) in these patients are increasing, and the association between irAEs and mogamulizumab remains to be elucidated. This study aimed to evaluate the association between mogamulizumab and immune-related adverse events (irAEs), as well as to characterize the irAEs associated with mogamulizumab using data from a large-scale spontaneous reporting system.<br> Methods: We performed an exploratory hypothesis-generating analysis of patients from 1967 to September 2023 using VigiBase, a World Health Organization spontaneous adverse event reporting system database. We performed a disproportionality analysis and determined the reporting odds ratios and information components between the drugs of interest and each irAE.<br> Results: Mogamulizumab was associated with some irAEs, including myocarditis, severe cutaneous adverse reactions, hepatitis, and myositis. Mogamulizumab exhibited significantly higher reporting rates of these 4 irAEs compared to the anticancer agents other than mogamulizumab. Conversely, the reporting rate of other irAEs, including endocrine autoimmune diseases induced by immune checkpoint inhibitors, was not significant in patients who received mogamulizumab.<br> Conclusions: Mogamulizumab is associated with irAEs, including myocarditis, severe cutaneous adverse reactions, hepatitis, and myositis. No potential conflict of interest relevant to this article was reported.

Iron and Steel Institute of Japan Acta Medica Okayama 0915-1559 65 13 2025 Heat Transfer Enhancement by Forming Bridges among Reactive Particles in a Packed Bed Reactor of a Solid-gas Chemical Heat Storage System 2097 2104 EN Koichi Nakaso Faculty of Environmental, Life Natural Science and Technology, Okayama University Kenji Shimada Graduate School of Natural Science and Technology, Okayama University Yasushi Mino Faculty of Environmental, Life Natural Science and Technology, Okayama University Kuniaki Gotoh Faculty of Environmental, Life Natural Science and Technology, Okayama University In this study, the enhancement of the thermal output of solid-gas chemical heat storage systems was investigated. Bridges made of high-thermal conductivity materials were formed among reactive particles by drying a slurry which contained graphite powder as a thermal additive and dispersant in a packed-bed reactor. First, the effect of the volume ratio of the dispersant on effective thermal conductivity was investigated. The optimum volume ratio of dispersant to graphite powder was determined. Furthermore, repetitive bridge formation increased the effective thermal conductivity. Based on these results, we investigated the thermal response of the energy-discharge process. Consequently, the temperature distribution in the radial direction of the reactor decreased owing to the formation of bridges. In addition, the thermal energy generated by the adsorption of water vapor onto the adsorbent was effectively transferred to the reactor wall. The thermal output was estimated based on the experimental results. The thermal output was increased by the formation of bridges. No potential conflict of interest relevant to this article was reported.

International Institute of Anticancer Research Acta Medica Okayama 0250-7005 46 1 2025 P53-Armed Oncolytic Virotherapy Promotes the Efficacy of PD1 Blockade in Murine Osteosarcoma Tumors 69 84 EN MIHO KURE Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences HIROSHI TAZAWA Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences KOJI DEMIYA Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences HIROYA KONDO Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences YUSUKE MOCHIZUKI Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences TADASHI KOMATSUBARA Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences AKI YOSHIDA Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences KOJI UOTANI Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences JOE HASEI Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences TOMOHIRO FUJIWARA Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences TOSHIYUKI KUNISADA Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences YASUO URATA Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences SHUNSUKE KAGAWA Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences TOSHIFUMI OZAKI Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences TOSHIYOSHI FUJIWARA Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Background/Aim: Osteosarcoma (OS) is refractory to immune checkpoint inhibitors targeting programmed cell death 1 (PD1)/PD ligand 1 (PD-L1) due to poor immune response. We previously developed telomerase-specific, replication-competent oncolytic adenoviruses non-armed OBP-301 and P53-armed OBP-702 that exert antitumor efficacy against human OS cells. Recently, we demonstrated that P53-armed OBP-702 induces more profound immunogenic cell death and antitumor immune response against human and murine OS cells than does non-armed OBP-301. In the present study, we assessed the combined efficacy of PD1 blockade and P53-armed OBP-702 against murine OS cells.<br> Materials and Methods: Three murine OS cell lines (K7M2, NHOS, NHOS-LM4) were used to assess the cytopathic effect of non-armed OBP-301 and P53-armed OBP-702 by XTT assay. Virus-induced immunogenic cell death was assessed by analyzing the levels of extracellular adenosine triphosphate and high-mobility group box protein B1. The expression of PD-L1 and PD-L2 was analyzed by flow cytometry. The malignant potential of NHOS-LM4 cells was analyzed by a migration and invasion assay. An orthotopic NHOS-LM4 tumor model was used to evaluate the antitumor efficacy of combination therapy with P53-armed OBP-702 and anti-PD1.<br> Results: P53-armed OBP-702 exhibited antitumor potential for the induction of immunogenic cell death, apoptosis, autophagy, and PD-L1/2 upregulation in K7M2 and NHOS cells. NHOS-LM4 cells showed increased migratory and invasive ability compared to NHOS cells. P53-armed OBP-702 significantly suppressed the malignant potential of NHOS-LM4 cells. Combination dosing showed that P53-armed OBP-702 significantly promoted the antitumor effect of PD1 blockade against NHOS-LM4 tumors.<br> Conclusion: Our results suggest that P53-armed OBP-702 is a promising agent for improving the antitumor effect of PD1 blockade in treating invasive OS. No potential conflict of interest relevant to this article was reported.

American Astronomical Society Acta Medica Okayama 0004-637X 992 1 2025 Observing Supernova Neutrino Light Curves with Super-Kamiokande. VI. A Practical Data Analysis Technique Considering Realistic Experimental Backgrounds 27 EN Fumi Nakanishi Department of Physics, Okayama University Ken’ichiro Nakazato Faculty of Arts and Science, Kyushu University Masayuki Harada Kamioka Observatory, Institute for Cosmic Ray Research, The University of Tokyo Yusuke Koshio Department of Physics, Okayama University Ryuichiro Akaho Faculty of Science and Engineering, Waseda University Yosuke Ashida Department of Physics, Tohoku University Akira Harada National Institute of Technology, Ibaraki College Masamitsu Mori Division of Science, National Astronomical Observatory of Japan Kohsuke Sumiyoshi National Institute of Technology, Numazu College Yudai Suwa Department of Earth Science and Astronomy, The University of Tokyo Roger A. Wendell Kavli Institute for the Physics and Mathematics of the Universe (Kavli IPMU, WPI), Todai Institutes for Advanced Study, The University of Tokyo Masamichi Zaizen Department of Earth Science and Astronomy, The University of Tokyo Neutrinos from supernovae, especially those emitted during the late phase of core collapse, are essential for understanding the final stages of massive star evolution. We have been dedicated to developing methods for the analysis of neutrinos emitted during the late phase and observed at Super-Kamiokande (SK). Our previous studies have successfully demonstrated the potential of various analysis methods in extracting essential physical properties; however, the lack of background consideration has limited their practical application. In this study, we address this issue by incorporating a realistic treatment of the experimental signal and background events with the on-going SK experiment. We therefore optimize our analysis framework to reflect realistic observational conditions, including both signal and background events. Using this framework we study several long-time supernova models, simulating the late phase neutrino observation in SK and focusing in particular on the identification of the last observed event. We discuss the possibility of model discrimination methods using timing information from this last observed event. No potential conflict of interest relevant to this article was reported.

AIP Publishing Acta Medica Okayama 0021-9606 163 22 2025 Fourier-transform infrared spectroscopy of hydrogen fluoride dimers in solid parahydrogen 224312 EN Yuki Miyamoto Research Institute for Interdisciplinary Science, Okayama University Hiroki Ooe Graduate School of Natural Science and Technology, Okayama University Susumu Kuma Department of Physics, Rikkyo University We investigate the Fourier-transform infrared spectra of hydrogen fluoride dimers in solid parahydrogen, the detailed analysis of which has remained unexplored. We propose a plausible analysis based on concentration dependence, light polarization, annealing, and time evolution. The absorption lines exhibited multiple peaks, with intensity ratios significantly altered by annealing and by time evolution at a constant temperature. The spectral patterns and isotopic effects suggest that the dimers do not rotate freely in solid parahydrogen, while multiple peaks arise from different stable structures, including single and double substitution sites. Unlike in the gas phase and helium droplets, no tunneling splitting was observed. The broad ν1 band suggests that some dimer structures may exhibit axial rotation. Spectral changes due to annealing likely result from site conversion, while observed IR-induced changes indicate preferential dissociation of dimers in double substitution sites. These findings still remain tentative, necessitating further experimental and theoretical studies. No potential conflict of interest relevant to this article was reported.

Pharmaceutical Society of Japan Acta Medica Okayama 0009-2363 73 5 2025 New Nucleoside Derivatives for Hybridization-Assisted Catalysis of Site-Selective Acetylation of 2′-OH of RNA 457 466 EN Hayate Takasaki Graduate School of Pharmaceutical Sciences, Kyushu University Kentaro Kitazaki Graduate School of Pharmaceutical Sciences, Kyushu University Yurie Hadano Graduate School of Pharmaceutical Sciences, Kyushu University Hirotaka Murase Faculty of Pharmaceutical Sciences, Sojo University Jeongsu Lee Graduate School of Pharmaceutical Sciences, Nagasaki International University Yosuke Taniguchi Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Shigeki Sasaki Graduate School of Pharmaceutical Sciences, Kyushu University New nucleoside derivatives containing the imidazole (Imd), pyridine or pyrimidine catalytic group were designed for site-specific acetylation of 2′-OH of the RNA ribose moiety. When the RNA substrate was acetylated in the presence of acetic anhydride under alkaline conditions, Probe (Imd) containing the imidazole catalytic group acetylated with a high selectivity to the 2′-OH of the uridine opposite the catalytic nucleotide. Probe (Py-4N) containing the pyridine group showed a higher catalytic activity under neutral conditions with a high selectivity for the 2′-OH group of the 5′ side of the uridine opposite the catalytic nucleotide in about 80% modification yield within 10 min. This study has shown that the oligodeoxynucleotide incorporating the new nucleotide derivative with the catalytic group can be a useful tool for site-selective acetylation of RNA 2′-OH. No potential conflict of interest relevant to this article was reported.

American Chemical Society (ACS) Acta Medica Okayama 2155-5435 15 14 2025 Mesoporous Oxyhalide Aggregates Exhibiting Improved Photocatalytic Activity for Visible-Light H2 Evolution and CO2 Reduction 12551 12562 EN Hiroto Ueki Department of Chemistry, School of Science, Institute of Science Tokyo Toshiya Tanaka Department of Chemistry, School of Science, Institute of Science Tokyo Shuji Anabuki Graduate School of Natural Science and Technology, Okayama University Ryuichi Nakada Department of Chemistry, School of Science, Institute of Science Tokyo Megumi Okazaki Department of Chemistry, School of Science, Institute of Science Tokyo Kenta Aihara Department of Chemistry, School of Science, Institute of Science Tokyo Masashi Hattori Materials and Structures Laboratory, Institute of Integrated Research, Institute of Science Tokyo Fumitaka Ishiwari Department of Applied Chemistry, Graduate School of Engineering, Osaka University Rie Haruki Institute of Materials Structure Science, High Energy Accelerator Research Organization Shunsuke Nozawa Institute of Materials Structure Science, High Energy Accelerator Research Organization Toshiyuki Yokoi Nanospace Catalysis Unit, Institute of Integrated Research, Institute of Science Tokyo Michikazu Hara Materials and Structures Laboratory, Institute of Integrated Research, Institute of Science Tokyo Osamu Ishitani Department of Chemistry, Graduate School of Advanced Science and Engineering, Hiroshima University Akinori Saeki Department of Applied Chemistry, Graduate School of Engineering, Osaka University Akira Yamakata Graduate School of Natural Science and Technology, Okayama University Kazuhiko Maeda Department of Chemistry, School of Science, Institute of Science Tokyo Oxyhalides are promising visible-light photocatalysts for water splitting and CO2 conversion; however, those exhibiting high activity for these reactions have rarely been reported. Here, we show that using water-soluble Ti complexes as precursors in the microwave-assisted hydrothermal synthesis of the oxyhalide photocatalyst Pb2Ti2O5.4F1.2 (PTOF) resulted in the production of nanoparticulate PTOF. The primary particle size of the synthesized PTOF ranged from several tens of nanometers to several hundreds of nanometers. Using Ti-citric acid or Ti-tartaric acid complexes as precursors, the PTOF was formed as mesoporous aggregates, compared with a bulky analogue (0.5–1 μm) prepared using a TiCl4 precursor. The PTOF prepared from Ti-citric acid complex had a particle size of 50–100 nm and showed a one-order-of-magnitude greater activity for H2 evolution from an aqueous ethylenediaminetetraacetic acid solution with the aid of a Rh cocatalyst. An apparent quantum yield (AQY) of 15.4 ± 1.0% at 420 nm, which is the highest among the reported oxyhalide photocatalysts, was achieved under optimal conditions. Although excess particle size reduction of PTOF lowered the H2 evolution activity, the PTOF with the smallest possible primary particle size of 15–30 nm, prepared from Ti-tartaric acid complex, showed the highest activity toward the selective reduction of CO2 into formate in a nonaqueous environment when combined with a binuclear Ru(II) complex. The CO2 reduction AQY was 10.4 ± 1.8% at 420 nm, a record-high value among metal-complex/semiconductor binary hybrid photocatalysts. This study highlights the importance of morphological control of oxyhalides for realizing their full potential as photocatalysts for artificial photosynthesis. No potential conflict of interest relevant to this article was reported.

American Chemical Society (ACS) Acta Medica Okayama 2574-0962 8 6 2025 Effects of Metal-Cation Doping on Photocatalytic H2 Evolution Activity of Layered Perovskite Oxynitride K2LaTa2O6N 3541 3552 EN Hideya Tsuchikado Department of Chemistry, School of Science, Institute of Science Tokyo Shuji Anabuki Graduate School of Natural Science and Technology, Okayama University Ovidiu Cretu Electron Microscopy Group, National Institute for Materials Science (NIMS) Yuki Kinoshita Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Institute of Science Tokyo Masashi Hattori Institute of Integrated Research, Institute of Science Tokyo Yuta Shiroma Department of Chemistry, School of Science, Institute of Science Tokyo Dongxiao Fan Institute of Materials Structure Science High Energy Accelerator Research Organization Megumi Okazaki Department of Chemistry, School of Science, Institute of Science Tokyo Takuto Soma Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Institute of Science Tokyo Fumitaka Ishiwari Department of Applied Chemistry, Graduate School of Engineering, Osaka University Shunsuke Nozawa Institute of Materials Structure Science High Energy Accelerator Research Organization Toshiyuki Yokoi Institute of Integrated Research, Institute of Science Tokyo Michikazu Hara Institute of Integrated Research, Institute of Science Tokyo Koji Kimoto Electron Microscopy Group, National Institute for Materials Science (NIMS) Akira Yamakata Graduate School of Natural Science and Technology, Okayama University Akinori Saeki Department of Applied Chemistry, Graduate School of Engineering, Osaka University Kazuhiko Maeda Department of Chemistry, School of Science, Institute of Science Tokyo Aliovalent cation doping into a heterogeneous photocatalyst affects several of its physicochemical properties, including its morphological characteristics, optical absorption behavior, and charge carrier dynamics, causing a drastic change in its photocatalytic activity. In the present work, we investigated the effects of aliovalent cation doping on the visible-light H2-evolution photocatalytic activity of the Ruddlesden–Popper layered perovskite oxynitride K2LaTa2O6N. The photocatalytic activity toward H2 evolution from an aqueous NaI solution was found to be enhanced by an increase in the specific surface area of the K2LaTa2O6N photocatalyst, which could be realized upon doping with lower-valence cations (e.g., Mg2+, Al3+, and Ga3+). Among the dopants examined at 1 mol % doping, Ga resulted in the highest activity. The activity of the Ga-doped specimen was further improved with increasing Ga concentration, where the maximal activity was obtained at 10 mol %, corresponding to an apparent quantum yield of 2.7 ± 0.4% at 420 nm from aqueous methanol. This number is the highest reported for a layered oxynitride photocatalyst. In the Ga-doped K2LaTa2O6N, a trade-off was observed between the Ga concentration and the photocatalytic activity. Although doping with Ga reduced the particle size of K2LaTa2O6N and suppressed undesirable charge recombination, it led to an enlarged bandgap, unsuitable for visible-light absorption. No potential conflict of interest relevant to this article was reported.

Biophysical Society of Japan Acta Medica Okayama 2189-4779 22 3 2025 Origin of the unique topology of the triangular water cluster in &lt;i&gt;Rubrobacter xylanophilus&lt;/i&gt; rhodopsin e220018 EN Tomoyasu Noji Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo Masaki Tsujimura Department of Advanced Interdisciplinary Studies, The University of Tokyo Keisuke Saito Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo Keiichi Kojima Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Yuki Sudo Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hiroshi Ishikita Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo The crystal structure of Rubrobacter xylanophilus rhodopsin (RxR) reveals a triangular cluster of three water molecules (W413, W415, and W419) at the extracellular proton-release site, near Glu187 and Glu197. Using a quantum mechanical/molecular mechanical approach, we identified the structural nature of this unique water cluster. The triangular shape is best reproduced when all three water molecules are neutral H2O with protonated Glu187 and deprotonated Glu197. Attempts to place H3O+ at any of these water molecules result in spontaneous proton transfer to one of the acidic residues and significant distortion from the crystal structure. The plane defined by the triangular water cluster extends into the guanidinium plane of Arg71, with both aligned along the W413...W419 axis. This extended plane lies nearly perpendicular to a five-membered, ring-like H-bond network involving two carboxyl oxygen atoms from Glu187 and one from Glu197. The resulting bipartite planar architecture, defined by the water triangle, Arg71, and the Glu187/Glu197 network may reflect the exceptional thermal stability in RxR. No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 0883-2927 187 2025 Nitrogen distribution and nitrogen isotope fractionation in synthetic 2:1 phyllosilicates under hydrothermal conditions at 200 °C and saturated vapor pressure 106403 EN Jaeguk Jo Graduate School of Natural Science and Technology, Okayama University Toshiro Yamanaka Graduate School of Natural Science and Technology, Okayama University Youko Miyoshi Research Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST) Masaya Suzuki Research Institute for Geo-Resources and Environment, Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST) Yoshihiro Kuwahara Department of Environmental Changes, Faculty of Social and Cultural Studies, Kyushu University Isao Kadota Graduate School of Natural Science and Technology, Okayama University Hitoshi Chiba Graduate School of Natural Science and Technology, Okayama University Bum Han Lee Critical Minerals Research Center, Korea Institute of Geoscience & Mineral Resources (KIGAM) This study investigates nitrogen distribution and isotope fractionation within synthetic 2:1 phyllosilicates, simulating submarine hydrothermal environments at 200 °C and saturated vapor pressure. XRD and EDS results revealed the potential coexistence of multiple cations in the interlayer of synthetic 2:1 phyllosilicate, concurrently suggesting cation substitution in the tetrahedral and/or octahedral sheets. Meanwhile, the iron-enriched 25-5 sample exhibited restricted interlayer expansibility. NH4+ absorptions were identified in the NH4-stretching (3200–2800 cm−1) and NH4-bending (1450–1400 cm−1) regions, with wavenumber shifts indicating the influence of interlayer water removal. At pH 10.56, over 95% of nitrogen was released into the gas phase, while at pH 8.88, nitrogen proportions in the liquid and gas phases were comparable (average 48–49%). Experiments with iron at pH ∼8.80 showed that the nitrogen proportion in the gas phase (average 28%) was more than twofold lower than that in the liquid phase (average 68%). Equilibrium isotope fractionation factors indicated discernible preference for heavier nitrogen isotopes in the solid phase (αsolid-liquid = 1.009–1.021 and αsolid-gas = 1.011–1.027). The αliquid-gas range for sample 25–2 was 1.001–1.008, while that for the iron-enriched composite 25–5 was 0.997–1.010. Our experimental studies have confirmed that, in the absence of exchange interactions with external substances possessing different nitrogen isotope ratios, nitrogen isotope fractionation between ammonium and ammonia, controlled by variations in temperature and pH during mineralization, plays a crucial role in the variation of nitrogen isotope ratios. Additionally, we confirmed that metal-amines influence nitrogen isotope fractionation by modulating ammonia gas emission. These findings enhance our understanding of nitrogen cycling across the gas, liquid, and solid phases in submarine hydrothermal systems. No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 2352-4855 90 2025 Species-specific sensitivity of marine phytoplankton to selected herbicides and antibiotics 104413 EN Shizuka Ohara Graduate School of Integrated Science for Life, Hiroshima University Toshimitsu Onduka Hatsukaichi Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency Shoko Ueki Institute of Plant Science and Resources, Okayama University Shotaro Naruse Graduate School of Integrated Science for Life, Hiroshima University Kazuhiko Koike Graduate School of Integrated Science for Life, Hiroshima University The toxicity of two herbicides (diuron and bromacil) and three antibiotics (clarithromycin, azithromycin, and clindamycin) was evaluated for on four marine phytoplankton species: two diatoms, Skeletonema costatum and Chaetoceros lorenzianus, a dinoflagellate, Prorocentrum shikokuense, and a raphidophyte, Heterosigma akashiwo. The 50 % effective concentrations (EC50-μ) for growth of the herbicides (approximately 2.3–24.3 μg L−1) were lower than those of the antibiotics, indicating their higher toxicity. The EC50-μ of diuron was close to its reported environmental concentrations. The EC50-μ values for the antibiotics substantially differed by species, ranging from 19.5 to > 1000 μg L−1, with diatoms showing higher sensitivity than flagellates. Herbicides inhibited the photosynthetic yield (φII) of all tested species at concentrations similar to or lower than those affecting growth, while antibiotics affected φII at higher concentrations. Under high-light conditions, photosynthesis in S. costatum was substantially inhibited by all chemicals except clindamycin, suggesting enhanced chemical toxicity under intense light. Overall, these findings indicate that these herbicides and antibiotics can alter phytoplankton abundance and composition in coastal areas and that environmental factors, such as increased solar radiation, can potentially enhance their toxicity. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Geochemical characterization of gaseous reservoirs in the enstatite-chondrite forming-region of the proto-solar nebula: Constraints from Li-isotope, O-isotope, and trace-element compositions in chondrule components EN TORII PHILIP DOUGLAS-SONG Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Development of a production method for biologically active globular proteins through chemical modification-based solubilization of denatured proteins EN Shuichiro KIMURA Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 ADAR1-high tumor-associated macrophages induce drug resistance and are therapeutic targets in colorectal cancer EN Hibiki OTSUKI (UMEDA) Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 2399-3669 8 1 2025 Synthesis of thienoacenes by electrochemical double C–S cyclization using a halogen mediator 366 EN Koichi Mitsudo Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Takuya Nagahara Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Nozomi Kataura Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Yuka Okamura Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Toki Yonezawa Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Yuri Tachibana Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Nolan Soulié Faculty of Science and Engineering, Sorbonne Université Keisuke Shigemori Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Eisuke Sato Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Hiroki Mandai Department of Pharmacy, Faculty of Pharmacy, Gifu University of Medical Science Seiji Suga Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Thienoacenes are significant compounds as organic materials. One of the most efficient ways to synthesize thienoacenes is to form multiple C–S bonds in a single step. Because unprotected S–H bonds are easily oxidized to S–S bonds, S-Me protected substrates are commonly used for the purpose. However, their reactivity is insufficient, and one-step construction of multiple C–S bonds is still challenging. We herein report the electrochemical synthesis of thienoacenes from S-methoxymethyl (MOM)-protected diarylacetylenes. In the presence of Bu4NBr as a halogen mediator, electrochemical double C–S cyclization of diarylacetylenes bearing two MOM groups proceeded to afford [1]benzothieno[3,2-b][1]benzothiophene (BTBT) derivatives. While S-Me or S-p-methoxybenzyl (PMB)-protected diarylacetylenes did not afford BTBT, BTBT was selectively obtained when a substrate protected with S-MOM groups was used. The S-MOM protection strategy is also effective for the electrochemical synthesis of a more π-expanded thienoacene such as dibenzo[d,d′]thieno[3,2-b,4,5-b′]dithiophene (DBTDT). No potential conflict of interest relevant to this article was reported.

Wiley Acta Medica Okayama 1464-6722 26 11 2025 Comparative Genomic Analysis Identifies FleQ and GcbB as Virulence-Associated Factors in Pseudomonas syringae pv. tabaci Strains e70168 EN Muhammad Taufiq Hidayat Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Kei Yoshioka Faculty of Agriculture, Okayama University Takafumi Nishimura Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Shuta Asai Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Sachiko Masuda Center for Sustainable Resource Science, RIKEN-TRIP Ken Shirasu Center for Sustainable Resource Science, RIKEN-TRIP Nanami Sakata Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Mikihiro Yamamoto Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Yoshiteru Noutoshi Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Kazuhiro Toyoda Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Yuki Ichinose Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Hidenori Matsui Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Pseudomonas syringae pv. tabaci (Pta) is an important plant pathogen, which causes wildfire disease in Nicotiana species. However, the genetic basis underlying strain-level differences in virulence remains largely unresolved. To address this, we performed a comparative genomic analysis between a highly virulent strain Pta6605 and a less virulent strain Pta7375. Despite high overall genome similarity, we identified key single-nucleotide polymorphisms, including premature stop-codon mutations in seven open reading frames in Pta7375. Notably, point mutations in two regulatory genes, such as fleQ, which encodes a transcription factor essential for flagellar biogenesis and biofilm formation, and gcbB, which encodes a GGDEF domain-containing diguanylate cyclase responsible for cyclic dimeric guanosine monophosphate (c-di-GMP) synthesis, were implicated in virulence disparity. Functional analyses using deletion and locus replacement mutants in the Pta6605 background revealed that the disruption of fleQ markedly reduced motility, flagellin production, c-di-GMP accumulation, biofilm formation and virulence level mirroring the Pta7375 phenotype. The gcbB replacement mutant showed reduced disease symptom development, although c-di-GMP levels remained comparable to the Pta6605 wild type. Locus replacement between strains confirmed that a point mutation in fleQ was the primary driver of reduced motility and flagellin expression in Pta7375. These findings indicate that the reduced virulence of Pta7375 is associated with impaired regulation of flagella-related genes and disruption of the FleQ-mediated c-di-GMP signalling, underscoring the value of comparative genomics in disentangling the complex regulatory networks that govern virulence in plant pathogens. No potential conflict of interest relevant to this article was reported.

American Physical Society (APS) Acta Medica Okayama 0031-9007 135 10 2025 Terahertz Field Control of Electronic-Ferroelectric Anisotropy at Room Temperature in LuFe2⁢O4 106504 EN Hirotake Itoh Tohoku University Ryusei Minakami Tohoku University Hongwu Yu Institute of Science Tokyo Ryohei Tsuruoka Tohoku University Tatsuya Amano Tohoku University Yohei Kawakami Tohoku University Shin-ya Koshihara Institute of Science Tokyo Kosuke Fujiwara Okayama University Naoshi Ikeda Okayama University Yoichi Okimoto Institute of Science Tokyo Shinichiro Iwai Tohoku University Electronic ferroelectrics, with polarization 𝑷 induced by strongly correlated charges, are expected to show ultrafast, huge, and flexible responses required in future optoelectronics. Although the challenges for ultrafast manipulation of such a polarization are ongoing, the expected advantages have been unclear. In this Letter, we demonstrate an unprecedentedly large increase by a factor of 2.7 in optical second harmonic generation at room temperature in the prototypical electronic ferroelectrics, the rare-earth ferrite LuFe2⁢O4, by applying a terahertz field of 260  kV/cm. The transient anisotropy indicates that the direction of macroscopic polarization can be controlled three dimensionally on subpicosecond timescales, offering additional degrees of freedom in controlling polarization. Although the polarization response is in phase concerning the terahertz field, its sensitivity increased with delay, indicating that cooperative interactions among microscopic domains play an important role in the unprecedented response. No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 0916-7005 2025 Refinement of interval approximations for fully commutative quivers EN Yasuaki Hiraoka Kyoto University Ken Nakashima Shimane University Ippei Obayashi Okayama University Chenguang Xu Kyoto University A central challenge in the theory of multiparameter persistence modules lies in defining effective descriptors for representations of infinite or wild type. In this work, we propose a novel framework for analyzing interval approximations of fully commutative quivers, which offers a tunable trade-off between approximation resolution and computational complexity. Our approach is evaluated on commutative ladder modules of both finite and infinite type. For finite-type cases, we establish an efficient method for computing indecomposable decompositions using solely one-parameter persistent homology. For infinite-type cases, we introduce a new invariant that captures persistence in the second parameter by connecting standard persistence diagrams through interval approximations. Furthermore, we present several models for constructing commutative ladder filtrations, providing new insights into the behavior of random filtrations and demonstrating the utility of our framework in topological analysis of material structures. No potential conflict of interest relevant to this article was reported.

Royal Society of Chemistry (RSC) Acta Medica Okayama 2050-7488 13 35 2025 Thermally polymerizable phthalocyanine realizes a metal–nitrogen-doped carbon material featuring a defined single-atom catalyst motif with CO2RR activity 28887 28895 EN Yuki Sano Department of Chemistry, Graduate School of Science, Tohoku University Daichi Nakajima Department of Chemistry, Graduate School of Science, Tohoku University Biplab Manna Center for Basic Research on Materials, National Institute for Materials Science Koki Chida Institute of Multidisciplinary Research for Advanced Materials, Tohoku University Ryojun Toyoda Department of Chemistry, Graduate School of Science, Tohoku University Shinya Takaishi Department of Chemistry, Graduate School of Science, Tohoku University Kazuyuki Iwase Institute of Multidisciplinary Research for Advanced Materials, Tohoku University Koji Harano Center for Basic Research on Materials, National Institute for Materials Science Yuta Nishina Graduate School of Natural Science and Technology, Okayama University Takeharu Yoshii Institute of Multidisciplinary Research for Advanced Materials, Tohoku University Ryota Sakamoto Department of Chemistry, Graduate School of Science, Tohoku University Metal–nitrogen-doped carbon materials (MNCs) exhibit good electrocatalytic performance owing to the intrinsic advantages of carbon-based materials and the presence of isolated and stabilized metal atoms coordinated by nitrogen sites. However, conventional high-temperature pyrolysis of precursor molecules make it difficult to control the coordination structure precisely. To address this issue, here we report a new synthesis strategy for MNCs. Specifically, we design and synthesize Ni-phthalocyanine functionalized with ethynyl groups as solid-state thermal polymerization points. After depositing the Ni-phthalocyanine precursor on a carbon support and performing a thermal treatment, the resultant carbon composite material features a Ni–N4 coordination structure derived from the precursor, and enhanced porosity. This material demonstrates high catalytic activity for the CO2 reduction reaction (CO2RR). Our synthetic approach is applicable to various precursor molecules and carbon supports, paving the way for the further development of MNC-based electrode catalysts. No potential conflict of interest relevant to this article was reported.

IOP Publishing Acta Medica Okayama 2399-6528 8 6 2024 Inert structural transition in 4H and 6H SiC at high pressure and temperature: a Raman spectroscopy study 065001 EN Shuhou Maitani Department of Physics, School of Science and Technology, Meiji University Ryosuke Sinmyo Department of Physics, School of Science and Technology, Meiji University Takayuki Ishii Institute for Planetary Materials, Okayama University Kenji Yoza Bruker Japan We conducted Raman spectroscopy measurements of 4H-SiC and 6H-SiC up to 69 GPa and 1023 K to assess the stability and bonding of SiC at high pressure and temperature. Both optic and acoustic modes were observed at wide pressure and temperature ranges. The temperature shifts of the Raman frequencies were fitted by the equation with the Bose–Einstein distribution function, and we found that the shifts were almost insensitive to the pressure. The mode Grüneisen coefficients weakly depend on the pressure and temperature, suggesting the sluggish transition of the crystal structure, unlike the previous experiments showing the transition or decomposition of SiC at high pressure and temperature conditions. Inert transitions are confirmed by Raman measurements and annealing experiments using multiple high-pressure apparatuses. The crystallinity may be a hidden critical parameter in the experiments to determine the stable polytypes of SiC under high pressure and temperature. No potential conflict of interest relevant to this article was reported.

American Chemical Society (ACS) Acta Medica Okayama 2691-3704 5 2 2025 Mechanistic Insights Into Oxidative Response of Heat Shock Factor 1 Condensates 606 617 EN Soichiro Kawagoe Institute of Advanced Medical Sciences, Tokushima University Motonori Matsusaki Institute of Advanced Medical Sciences, Tokushima University Takuya Mabuchi Frontier Research Institute for Interdisciplinary Sciences, Tohoku University Yuto Ogasawara Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kazunori Watanabe Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University Koichiro Ishimori Department of Chemistry, Faculty of Science, Hokkaido University Tomohide Saio Institute of Advanced Medical Sciences, Tokushima University Heat shock factor 1 (Hsf1), a hub protein in the stress response and cell fate decisions, senses the strength, type, and duration of stress to balance cell survival and death through an unknown mechanism. Recently, changes in the physical property of Hsf1 condensates due to persistent stress have been suggested to trigger apoptosis, highlighting the importance of biological phase separation and transition in cell fate decisions. In this study, the mechanism underlying Hsf1 droplet formation and oxidative response was investigated through 3D refractive index imaging of the internal architecture, corroborated by molecular dynamics simulations and biophysical/biochemical experiments. We found that, in response to oxidative conditions, Hsf1 formed liquid condensates that suppressed its internal mobility. Furthermore, these conditions triggered the hyper-oligomerization of Hsf1, mediated by disulfide bonds and secondary structure stabilization, leading to the formation of dense core particles in the Hsf1 droplet. Collectively, these data demonstrate how the physical property of Hsf1 condensates undergoes an oxidative transition by sensing redox conditions to potentially drive cell fate decisions. No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 0340-5354 272 1 2024 Genetic and functional analyses of SPTLC1 in juvenile amyotrophic lateral sclerosis 36 EN So Okubo Department of Neurology, Graduate School of Medicine, The University of Tokyo Hiroya Naruse Department of Neurology, Graduate School of Medicine, The University of Tokyo Hiroyuki Ishiura Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Atsushi Sudo Department of Neurology, Graduate School of Medicine, The University of Tokyo Kayoko Esaki Department of Biotechnology and Life Sciences, Faculty of Biotechnology and Life Sciences, Sojo University Jun Mitsui Department of Neurology, Graduate School of Medicine, The University of Tokyo Takashi Matsukawa Department of Neurology, Graduate School of Medicine, The University of Tokyo Wataru Satake Department of Neurology, Graduate School of Medicine, The University of Tokyo Peter Greimel Laboratory for Cell Function Dynamics, RIKEN Centre for Brain Sciences Nanoka Shingai Division of Applied Life Science, Graduate School of Engineering, Sojo University Yasushi Oya Department of Neurology, National Center of Neurology and Psychiatry Takeo Yoshikawa Laboratory of Molecular Psychiatry, RIKEN Center for Brain Science Shoji Tsuji Department of Neurology, Graduate School of Medicine, The University of Tokyo Tatsushi Toda Department of Neurology, Graduate School of Medicine, The University of Tokyo Introduction Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder of the motor system. Pathogenic variants in SPTLC1, encoding a subunit of serine palmitoyltransferase, cause hereditary sensory and autonomic neuropathy type 1 (HSAN1), and have recently been associated with juvenile ALS. SPTLC1 variants associated with ALS cause elevated levels of sphinganines and ceramides. Reports on ALS associated with SPTLC1 remain limited. This study aimed to investigate the frequency of SPTLC1 variants in ALS and relevant clinical characteristics.<br> Methods We analyzed whole-exome and whole-genome sequence data from 40 probands with familial ALS and 413 patients with sporadic ALS without previously identified causative variants. Reverse transcription polymerase chain reaction (RT-PCR) analysis and droplet digital PCR (ddPCR) were used to assess splicing and mosaicism, respectively. Plasma sphingolipid levels were quantified to analyze biochemical consequences.<br> Results The heterozygous c.58G>A, p.Ala20Thr variant was identified in a 21-year-old Japanese female patient presenting with symmetric weakness which slowly progressed over 15 years. RT-PCR analysis showed no splice defects. Plasma sphingolipid levels in the patient were significantly increased compared to her asymptomatic parents. ddPCR revealed that the asymptomatic father harbored a mosaic variant with 17% relative mutant allele abundance in peripheral blood leukocytes.<br> Conclusions We identified a pathogenic c.58G>A, p.Ala20Thr SPTLC1 variant in a patient with juvenile ALS, likely inherited from an asymptomatic parent with mosaicism. Lipid analysis results are consistent with previous findings on SPTLC1-associated ALS. Further studies are necessary to determine the clinical effect of mosaic variants of SPTLC1. No potential conflict of interest relevant to this article was reported.

American Chemical Society (ACS) Acta Medica Okayama 2691-3704 2025 Electrochemical Generation of Sulfonamidyl Radicals via Anodic Oxidation of Hydrogen Bonding Complexes: Applications to Electrosynthesis of Benzosultams EN Yasuyuki Okumura Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Eisuke Sato Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Koichi Mitsudo Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Seiji Suga Division of Applied Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University Amidyl radicals and sulfonamidyl radicals are widely used in the field of organic synthesis. In particular, the electrochemical oxidation of amides in the presence of bases is one of the most practical methods for generating amidyl radicals. However, it is often difficult to observe the “true” radical precursor, such as an amide anion and/or a hydrogen bonding complex with an amide and a base. We found that a sulfonamide and Bu4NOAc form a 1:1 hydrogen bonding complex by spectroscopic experiments. Cyclic voltammetry suggested that 1:1 hydrogen bonding complexes should be oxidized predominantly under the optimized conditions to afford a sulfonamidyl radical via the proton-coupled electron transfer (PCET) process by the oxidation of the complex. Thus-generated sulfonamidyl radicals could be used in the electrochemical synthesis of a variety of benzosultams. No potential conflict of interest relevant to this article was reported.

Royal Society of Chemistry (RSC) Acta Medica Okayama 2050-7488 13 23 2025 A meta-linked isomer of ITIC: influence of aggregation patterns on open-circuit voltage in organic solar cells 17720 EN Kai Wang The Institute of Scientific and Industrial Research (SANKEN), The University of Osaka Seihou Jinnai The Institute of Scientific and Industrial Research (SANKEN), The University of Osaka Kaito Uesaka Graduate School of Natural Science & Technology, Okayama University Akira Yamakata Graduate School of Natural Science & Technology, Okayama University Yutaka Ie The Institute of Scientific and Industrial Research (SANKEN), The University of Osaka Improving the open-circuit voltage (VOC) of organic solar cells (OSCs) remains an important challenge. While it is known that the energy levels at the donor/acceptor (D/A) interface affect the VOC, the impact of aggregation patterns on the energy levels at the D/A interface has not been fully elucidated. Herein, we focus on ITIC, a widely used acceptor in OSCs, and designed a meta-linked isomer of ITIC (referred to as im-ITIC) to alter molecular symmetry and modify substitution arrangements. Concentration-dependent 1H NMR spectra revealed that im-ITIC shows stronger aggregation behavior in solution. Single-crystal X-ray analysis showed that im-ITIC forms both tail-to-tail (J-aggregation) and face-to-face (H-aggregation) stacking modes, whereas ITIC exclusively forms tail-to-tail stacking. OSCs based on PBDB-T:im-ITIC showed a high VOC value of 1.02 V, which is 0.12 V higher than that of those based on PBDB-T:ITIC. Time-resolved infrared measurements revealed the lifetime of free electrons for the pristine and blend films. The energy levels of the charge transfer state (ECT) for PBDB-T:im-ITIC- and PBDB-T:ITIC OSCs were determined to be 1.57 and 1.39 eV, respectively, correlating with the VOC values. Theoretical calculations indicated that pronounced H-aggregation in im-ITIC increases the ECT compared with J-aggregation, contributing to the improved VOC. This study underscores the critical impact of molecular aggregation patterns on energy alignment and VOC enhancement, offering insights into molecular design for achieving high VOC in OSCs. No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 0910-6340 41 7 2025 Direct insertion of an ion channel immobilized on a soft agarose gel bead into a lipid bilayer: an optimized method 1073 1082 EN Mami Asakura Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Shuyan Wang Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Minako Hirano Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Toru Ide Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University In this paper, we report the development of a device that improves the conventional artificial lipid bilayer method and can measure channel currents more efficiently. Ion channel proteins are an attractive research target in biophysics, because their functions can be measured at the single-molecule level with high time resolution. In addition, they have attracted attention as targets for drug discovery because of their crucial roles in vivo. Although electrophysiological methods are powerful tools for studying channel proteins, they suffer from low measurement efficiency and require considerable skill. In our previous paper, we reported that by immobilizing channel proteins on agarose gel beads and forming an artificial lipid bilayer on the bead surface, we simultaneously solved two problems that had been hindering the efficiency of the artificial bilayer method: the time-consuming formation of artificial lipid bilayers and the time-consuming incorporation of channels into artificial bilayers. Previous studies have utilized crosslinked hard beads; however, here we show that channel current measurement can be achieved more simply and efficiently using non-crosslinked soft beads. In this study, we detailed the process of immobilizing channel proteins on the surface of non-crosslinked beads through chemical modification, allowing us to measure their channel activity. This method enables current measurements without the need for stringent bead size selection or high negative pressure. No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 0910-6340 41 4 2024 Efficient single-channel current measurements of the human BK channel using a liposome-immobilized gold probe 329 334 EN Minako Hirano Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Mami Asakura Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Toru Ide Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University The human BK channel (hBK) is an essential membrane protein that regulates various biological functions, and its dysfunction leads to serious diseases. Understanding the biophysical properties of hBK channels is crucial for drug development. Artificial lipid bilayer recording is used to measure biophysical properties at the single-channel level. However, this technique is time-consuming and complicated; thus, its measurement efficiency is very low. Previously, we developed a novel technique to improve the measurement efficiency by rapidly forming lipid bilayer membranes and incorporating ion channels into the membrane using a hydrophilically modified gold probe. To further improve our technique for application to the hBK channel, we combined it using the gold probe with a liposome fusion method. Using a probe on which liposomes containing hBK channels were immobilized, the channels were efficiently incorporated into the lipid bilayer membrane, and the measured channel currents showed the current characteristics of the hBK channel. This technique will be useful for the efficient measurements of the channel properties of hBK and other biologically important channels. No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 0021-9258 301 7 2025 A repertoire of visible light–sensitive opsins in the deep-sea hydrothermal vent shrimp Rimicaris hybisae 110291 EN Yuya Nagata Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Norio Miyamoto Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Keita Sato Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Yosuke Nishimura Research Center for Bioscience and Nanoscience (CeBN), Research Institute for Marine Resources Utilization, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Yuki Tanioka School of Pharmaceutical Sciences, Okayama University Yuji Yamanaka School of Pharmaceutical Sciences, Okayama University Susumu Yoshizawa Atmosphere and Ocean Research Institute, The University of Tokyo Kuto Takahashi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kohei Obayashi Department of Biology, Graduate School of Science, Kobe University Hisao Tsukamoto Department of Biology, Graduate School of Science, Kobe University Ken Takai Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Hideyo Ohuchi Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Takahiro Yamashita Department of Biophysics, Graduate School of Science, Kyoto University Yuki Sudo Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Keiichi Kojima Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Unlike terrestrial environments, where humans reside, there is no sunlight in the deep sea. Instead, dim visible light from black-body radiation and bioluminescence illuminates hydrothermal vent areas in the deep sea. A deep-sea hydrothermal vent shrimp, Rimicaris hybisae, is thought to detect this dim light using its enlarged dorsal eye; however, the molecular basis of its photoreception remains unexplored. Here, we characterized the molecular properties of opsins, universal photoreceptive proteins in animals, found in R. hybisae. Transcriptomic analysis identified six opsins: three Gq-coupled opsins, one Opn3, one Opn5, and one peropsin. Functional analysis revealed that five of these opsins exhibited light-dependent G protein activity, whereas peropsin exhibited the ability to convert all-trans-retinal to 11-cis-retinal like photoisomerases. Notably, all the R. hybisae opsins, including Opn5, convergently show visible light sensitivity (around 457–517 nm), whereas most opsins categorized as Opn5 have been demonstrated to be UV sensitive. Mutational analysis revealed that the unique visible light sensitivity of R. hybisae Opn5 is achieved through the stabilization of a protonated Schiff base by a counterion residue at position 83 (Asp83), which differs from the position identified in other opsins. These findings suggest that the vent shrimp R. hybisae has adapted its photoreceptive devices to dim deep-sea hydrothermal light by selectively maintaining a repertoire of visible light–sensitive opsins, including the uniquely tuned Opn5. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 The evolutionary contextualization and deep neural network modeling on fruit ripening response EN Eriko KUWADA Graduate School of Environmental, Life, Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Baseline gut microbiota as a predictive marker for the efficacy of neoadjuvant chemotherapy in patients with early breast cancer: a multicenter prospective cohort study in the Setouchi Breast Project‑14 EN Shogo NAKAMOTO Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

MDPI Acta Medica Okayama 1661-6596 26 6 2025 Vesicular Glutamate Transporter 3 Is Involved in Glutamatergic Signalling in Podocytes 2485 EN Naoko Nishii Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Tomoko Kawai Department of Cell Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hiroki Yasuoka Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Tadashi Abe Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Nanami Tatsumi Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Yuika Harada Department of Genomics and Proteomics, Advanced Science Research Center, Okayama University Takaaki Miyaji Department of Genomics and Proteomics, Advanced Science Research Center, Okayama University Shunai Li Center for Innovative Clinical Medicine, Okayama University Hospital Moemi Tsukano Central Research Laboratory, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Masami Watanabe Center for Innovative Clinical Medicine, Okayama University Hospital Daisuke Ogawa Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Jun Wada Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kohji Takei Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hiroshi Yamada Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Glomerular podocytes act as a part of the filtration barrier in the kidney. The activity of this filter is regulated by ionotropic and metabotropic glutamate receptors. Adjacent podocytes can potentially release glutamate into the intercellular space; however, little is known about how podocytes release glutamate. Here, we demonstrated vesicular glutamate transporter 3 (VGLUT3)-dependent glutamate release from podocytes. Immunofluorescence analysis revealed that rat glomerular podocytes and an immortal mouse podocyte cell line (MPC) express VGLUT1 and VGLUT3. Consistent with this finding, quantitative RT-PCR revealed the expression of VGLUT1 and VGLUT3 mRNA in undifferentiated and differentiated MPCs. In addition, the exocytotic proteins vesicle-associated membrane protein 2, synapsin 1, and synaptophysin 1 were present in punctate patterns and colocalized with VGLUT3 in MPCs. Interestingly, approximately 30% of VGLUT3 colocalized with VGLUT1. By immunoelectron microscopy, VGLUT3 was often observed around clear vesicle-like structures in differentiated MPCs. Differentiated MPCs released glutamate following depolarization with high potassium levels and after stimulation with the muscarinic agonist pilocarpine. The depletion of VGLUT3 in MPCs by RNA interference reduced depolarization-dependent glutamate release. These results strongly suggest that VGLUT3 is involved in glutamatergic signalling in podocytes and may be a new drug target for various kidney diseases. No potential conflict of interest relevant to this article was reported.

岡山大学大学院法務研究科 Acta Medica Okayama 1881-1485 26 2025 非法務部門で働くインハウス−内部監査、監査役スタッフ業務の魅力− 113 120 EN Yuko Minami 10.18926/OLR/68569 No potential conflict of interest relevant to this article was reported.

岡山大学文明動態学研究所 Acta Medica Okayama 2436-8326 4 2025 吉備製鉄遺跡周辺地域の磁鉄鉱ざくろ石スカルン鉄鉱石の鉱物学的・地球化学的特徴 19 52 EN Yasushi TAKECHI Kurashiki Museum of Natural History Daisuke NAKAMURA Okayama University, Department of Earth Sciences Shigeyuki SUZUKI Okayama University, Department of Earth Sciences Joseph RYAN Okayama University, Research Institute for the Dynamics of Civilizations Takeshi UWAGAKI Okayama Prefectural Board of Education Masato NAGAHARA The Historical Study Group of Mining and Metallurgy of Japan Yuta YOSHIE Mitsui Mining & Smelting Co., Ltd. Kei IKEHATA University of Tsukuba, Faculty of Life and Environmental Sciences Osamu KIMURA Okayama University, Research Institute for the Dynamics of Civilizations Ryoichi HATTORI Osaka University, Graduate School of Humanities 論文 (Research article) 10.18926/67954 We conducted mineralogical and geochemical analysis of ore samples taken from locations surrounding the Jinmu, Sanpō, and Kōmoto mines in order to determine the source of iron ore uncovered from archaeological sites. The mineral composition of the magnetite-garnet bearing skarn deposits varies from mine to mine: while clinopyroxene and amphibole are present in the Jinmu and Sanpō samples, only a small amount of clinopyroxene occurs in the Kōmoto samples. The chemical compositions of magnetite and garnet are distinctive for each mine. Among the trace elements contained in the magnetite, Mg and Mn tend to be higher in the Kōmoto samples, Ti in the Jinmu samples, and Ca and Si in the Sanpō samples. The garnet from all the mines is andradite, but while the Kōmoto samples contain almost no Al, it is present in the Jinmu and Sanpō samples. Although samples were taken from a limited number of mine areas (three), our analysis provides an index for comparison with iron ore uncovered from archaeological sites, which will aid in provenance determination. No potential conflict of interest relevant to this article was reported.

MDPI Acta Medica Okayama 2073-4409 13 16 2024 Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes 1373 EN Hiroshi Yamada Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hirona Osaka Graduate School of Science, Nagoya University Nanami Tatsumi Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Miu Araki Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Tadashi Abe Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Keiko Kaihara Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Ken Takahashi Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Eizo Takashima Division of Malaria Research, Proteo-Science Center, Ehime University Takayuki Uchihashi Graduate School of Science, Nagoya University Keiji Naruse Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kohji Takei Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Synaptopodin 2-like protein (SYNPO2L) is localized in the sarcomere of cardiomyocytes and is involved in heart morphogenesis. However, the molecular function of SYNPO2L in the heart is not fully understood. We investigated the interaction of SYNPO2L with sarcomeric alpha-actinin and actin filaments in cultured mouse cardiomyocytes. Immunofluorescence studies showed that SYNPO2L colocalized with alpha-actinin and actin filaments at the Z-discs of the sarcomere. Recombinant SYNPO2La or SYNPO2Lb caused a bundling of the actin filaments in the absence of alpha-actinin and enhanced the alpha-actinin-dependent formation of actin bundles. In addition, high-speed atomic force microscopy revealed that SYNPO2La directly bound to alpha-actinin via its globular ends. The interaction between alpha-actinin and SYNPO2La fixed the movements of the two proteins on the actin filaments. These results strongly suggest that SYNPO2L cooperates with alpha-actinin during actin bundle formation to facilitate sarcomere formation and maintenance. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 One-Pot Synthesis of Chemicals Using CO2 as Chemical Feedstock EN Koichi NAKAOKA Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 Immunohistochemistry for IRTA1 and MNDA helps differentiate gastric MALT lymphoma from chronic gastritis/reactive lymphocyte hyperplasia EN Yoshiyuki AYADA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 Prospective observational study of zinc deficiency symptoms during first-line chemotherapy for gastric and colorectal cancer EN Chihiro SAKAGUCHI Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学文学部学芸員課程 Acta Medica Okayama 14 2020 学芸員課程 Newsletter 第14号 EN 10.18926/66818 No potential conflict of interest relevant to this article was reported.

岡山大学文学部学芸員課程 Acta Medica Okayama 12 2019 学芸員課程 Newsletter 第12号 EN 10.18926/66816 No potential conflict of interest relevant to this article was reported.

岡山大学文学部学芸員課程 Acta Medica Okayama 10 2018 学芸員課程 Newsletter 第10号 EN 10.18926/66813 No potential conflict of interest relevant to this article was reported.

岡山大学文学部学芸員課程 Acta Medica Okayama 8 2017 学芸員課程 Newsletter 第8号 EN 10.18926/66811 No potential conflict of interest relevant to this article was reported.

岡山大学文学部学芸員課程 Acta Medica Okayama 7 2016 学芸員課程 Newsletter 第7号 EN 10.18926/66810 No potential conflict of interest relevant to this article was reported.

岡山大学文学部学芸員課程 Acta Medica Okayama 6 2016 学芸員課程 Newsletter 第6号 EN 10.18926/66809 No potential conflict of interest relevant to this article was reported.

岡山大学文学部学芸員課程 Acta Medica Okayama 3 2014 学芸員課程 Newsletter 第3号 EN 10.18926/66806 No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 113 2024 過剰発現プロファイリングADOPT法を用いたS.cerevisiaeのワイン醸造用ブドウ果汁への適応性評価 1 6 EN Hisao Moriya Course of Agrochemical Bioscience Chiyuki Kohata Ono Course of Agrochemical Bioscience The authors have recently developed the overexpression profiling ADOPT method. In the ADOPT method, yeast strains overexpressing most of the genes in the budding yeast Saccharomyces cerevisiae genome are mixed and competitively cultured, and the genes overexpressed in the enriched strains are systematically identified. Furthermore, the identified genes can be used to identify bottleneck factors that are necessary but lacking for growth of S. cerevisiae under given conditions. In our previous studies, we have identified bottlenecks in artificially created stress environments in the laboratory, but in this study, we used grape juice for winemaking as an example to see if industrial bottlenecks can be identified. ADOPT experiments with sulfite-added grape juice used in conventional winemaking resulted in a strong enrichment of strains overexpressing the sulfite pump SSU1 and its transcription factor FZF1. Since enhancement of SSU1 function is known to occur in wine yeast acclimation, ADOPT was also shown to be useful in the search for industrial bottlenecks. On the other hand, no genes were strongly enriched by ADOPT in grape juice without sulfite addition, suggesting that grape juice is a balanced medium with few bottlenecks for S. cerevisiae growth. No potential conflict of interest relevant to this article was reported.

岡山大学大学院社会文化科学研究科 Acta Medica Okayama 1880-9162 23 2023 ライプニッツの物質論―化学を焦点に考える 23 44 EN Tsuyoshi MATSUDA 10.18926/66507 No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 135 3 2023 第55回日本結合組織学会学術大会開催報告 174 174 EN Toshitaka Oohashi Department of Molecular Biology and Biochemistry, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 化学療法を受ける大腸がん患者の手指に生じる末梢神経障害の特徴―横断的観察研究― EN Chika YASUDA Graduate School of Health Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Application of amorphous sugar matrix dried from alcohol to solid dispersion of hydrophobic drug and its physicochemical characteristics EN Koji TAKEDA Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Decreased expression of hyaluronan synthase and loss of hyaluronan-rich cells in the anterior tibial fascia of the rat model of chemotherapy-induced peripheral neuropathy EN RUILIN WANG Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Expression of Acetabular Labral Vascular Endothelial Growth Factor and Nerve Growth Factor Is Directly Associated with Hip Osteoarthritis Pain: Investigation by Immunohistochemical Staining EN Yoshihiro SATO Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2005 Molecular genetic analysis and biochemical characterization of multidrug efflux pumps in Gram negative bacteria EN Anowara Begum No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 135 2 2023 オートファジー 92 94 EN Kohji Takei Department of Neurosience, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hiroshi Yamada Department of Neurosience, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Photochemical properties of carbon nanotube/dendrimer hybrids for photocatalytic H2 evolution from water and biomedical imaging EN Masahiro YAMAGAMI Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Early Efforts for Iron Production in Central Anatolia: Geochemical Analysis of Iron-rich Stones, Slags and Metal Objects from the Bronze Age in Kaman-Kalehöyük (Turkey) EN KUCUKARSLAN NURCAN Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Chemical reactions induced by localized light and their application to metal nanofabrication EN Yuki TAKEUCHI Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Predictive value of immune genomic signatures from breast cancer cohorts containing data for both response to neoadjuvant chemotherapy and prognosis after surgery EN YIDAN ZHU Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 134 3 2022 令和３年度岡山医学会賞　脳神経研究奨励賞（新見賞） 139 141 EN Kojiro Nojima Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences No potential conflict of interest relevant to this article was reported.

日本口腔内科学会 Acta Medica Okayama 2186-6147 28 2 2022 「MASCC/ISOO 口腔粘膜障害のマネジメントに関する臨床ガイドライン（2019-2020改訂版）」公式日本語訳概要の解釈について 41 49 EN Yoshihiko SOGA Division of Hospital Dentistry, Okayama University Hospital がん治療における化学療法、口腔を照射野に含む放射線療法、および造血幹細胞移植では、有害事象として口腔粘膜障害がしばしば発生する。がん支持療法の国際学会であるMultinational Association of Supportive Care in Cancer および International Society for Oral Oncology (MASCC/ISOO)は、システマティックレビューの手法により、2019年から2020年にかけて、「MASCC/ISOO 口腔粘膜障害のマネジメントに関する臨床ガイドライン」の改訂版を発表した。さらに、総括の概要について、日本語の公式翻訳版も発表された。しかし、国際的なガイドラインであるが故に、日本での非承認薬あるいは適応外使用について言及されている内容が多い。また、総括の概要で、原文に由来する意図が伝わりにくい箇所がある。本稿では、改訂されたガイドラインの内容について紹介するとともに、上述した問題点を念頭に、ガイドラインの総括の概要に記載された内容について詳述した。 No potential conflict of interest relevant to this article was reported.

岡山大学経済学会 Acta Medica Okayama 2433-4146 54 3 2023 企業の人事考課制度に関するアンケート調査の報告 61 79 EN Noriko Hatada Yoichi Matsuda 10.18926/OER/65012 No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 112 2023 Streptomyces属放線菌由来の高基質特異性l-グルタミン酸オキシダーゼに関する研究 13 18 EN Natsume Nakayama Course of Agrochemical Bioscience K. Inagaki l-glutamate oxidase (LGOX) from Streptomyces sp. is a heterohexameric flavin enzyme that catalyzes the oxidative deamination of l-glutamate to form α-ketoglutarate with ammonia and hydrogen peroxide. LGOX shows strict substrate specificity for l-Glu. In addition, it is highly thermostable and pH stable. Because of these properties, LGOX is currently used as a biosensor for the trace determination of l-Glu in the food industry and clinical laboratories. The full-length cDNA is 2103 bp and is encoded by a single polypeptide chain consisting of 701 residues including subunits α-γ-β. The LGOX gene was heterologously expressed in Escherichia coli JM109. The LGOX precursor expressed in E. coli is a homodimer with weak enzymatic activity and becomes a heterohexamer upon activation by protease treatment. X-ray crystallography and docking studies of purified recombinant LGOX suggest that the Arg305 residue is a key residue for substrate recognition. Mutant analysis showed that Arg305 is essential for substrate recognition, as the activity toward l-Glu was greatly reduced and substrate specificity was changed in some enzymes. The functional analysis of R305E-LGOX, which is an l-Arg oxidase, revealed that R305E-LGOX can be used as a enzyme biosensor for l-Arg. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 The aetiology of mesothelioma: morphological and chemical analyses of asbestos ferruginous bodies EN AVRAMESCU MAYA LILIANA Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 Reconstructions of temporal and spatial variations in surface conditions of the Indonesian Seas based on geochemical tracers in modern coral skeletons EN Ai GENDA Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Frontiers Media Acta Medica Okayama 2235-2988 12 2022 Recruitment of Irgb6 to the membrane is a direct trigger for membrane deformation 992198 EN Hiroshi Yamada Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Tadashi Abe Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hikaru Nagaoka Division of Malaria Research, Proteo-Science Center, Ehime University Eizo Takashima Division of Malaria Research, Proteo-Science Center, Ehime University Ryo Nitta Division of Structural Medicine and Anatomy, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine Masahiro Yamamoto Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University Kohji Takei Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Irgb6 is a member of interferon gamma-induced immunity related GTPase (IRG), and one of twenty "effector" IRGs, which coordinately attack parasitophorous vacuole membrane (PVM), causing death of intracellular pathogen. Although Irgb6 plays a pivotal role as a pioneer in the process of PVM disruption, the direct effect of Irgb6 on membrane remained to be elucidated. Here, we utilized artificial lipid membranes to reconstitute Irgb6-membrane interaction in vitro, and revealed that Irgb6 directly deformed the membranes. Liposomes incubated with recombinant Irgb6 were drastically deformed generating massive tubular protrusions in the absence of guanine nucleotide, or with GMP-PNP. Liposome deformation was abolished by incubating with Irgb6-K275A/R371A, point mutations at membrane targeting residues. The membrane tubules generated by Irgb6 were mostly disappeared by the addition of GTP or GDP, which are caused by detachment of Irgb6 from membrane. Binding of Irgb6 to the membrane, which was reconstituted in vitro using lipid monolayer, was stimulated at GTP-bound state. Irgb6 GTPase activity was stimulated by the presence of liposomes more than eightfold. Irgb6 GTPase activity in the absence of membrane was also slightly stimulated, by lowering ionic strength, or by increasing protein concentration, indicating synergistic stimulation of the GTPase activity. These results suggest that membrane targeting of Irgb6 and resulting membrane deformation does not require GTP, but converting into GTP-bound state is crucial for detaching Irgb6 from the membrane, which might coincident with local membrane disruption. No potential conflict of interest relevant to this article was reported.

Frontiers Media SA Acta Medica Okayama 2296-634X 10 2022 The Lipid-Binding Defective Dynamin 2 Mutant in Charcot-Marie-Tooth Disease Impairs Proper Actin Bundling and Actin Organization in Glomerular Podocytes 884509 EN Eriko Hamasaki Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Natsuki Wakita Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hiroki Yasuoka Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hikaru Nagaoka Division of Malaria Research, Proteo-Science Center, Ehime University Masayuki Morita Division of Malaria Research, Proteo-Science Center, Ehime University Eizo Takashima Division of Malaria Research, Proteo-Science Center, Ehime University Takayuki Uchihashi Department of Physics, Nagoya University Tetsuya Takeda Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Tadashi Abe Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Ji-Won Lee Department of Pharmacology, Faculty and Graduate School of Dental Medicine, Hokkaido University Tadahiro Iimura Department of Pharmacology, Faculty and Graduate School of Dental Medicine, Hokkaido University Moin A. Saleem Bristol Renal, Translational Health Sciences, Bristol Medical School, University of Bristol Naohisa Ogo Center for Drug Discovery, Graduate School of Pharmaceutical Sciences, University of Shizuoka Akira Asai Center for Drug Discovery, Graduate School of Pharmaceutical Sciences, University of Shizuoka Akihiro Narita Graduate School of Science, Nagoya University Kohji Takei Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hiroshi Yamada Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Dynamin is an endocytic protein that functions in vesicle formation by scission of invaginated membranes. Dynamin maintains the structure of foot processes in glomerular podocytes by directly and indirectly interacting with actin filaments. However, molecular mechanisms underlying dynamin-mediated actin regulation are largely unknown. Here, biochemical and cell biological experiments were conducted to uncover how dynamin modulates interactions between membranes and actin in human podocytes. Actin-bundling, membrane tubulating, and GTPase activities of dynamin were examined in vitro using recombinant dynamin 2-wild-type (WT) or dynamin 2-K562E, which is a mutant found in Charcot-Marie-Tooth patients. Dynamin 2-WT and dynamin 2-K562E led to the formation of prominent actin bundles with constant diameters. Whereas liposomes incubated with dynamin 2-WT resulted in tubule formation, dynamin 2-K562E reduced tubulation. Actin filaments and liposomes stimulated dynamin 2-WT GTPase activity by 6- and 20-fold, respectively. Actin-filaments, but not liposomes, stimulated dynamin 2-K562E GTPase activity by 4-fold. Self-assembly-dependent GTPase activity of dynamin 2-K562E was reduced to one-third compared to that of dynamin 2-WT. Incubation of liposomes and actin with dynamin 2-WT led to the formation of thick actin bundles, which often bound to liposomes. The interaction between lipid membranes and actin bundles by dynamin 2-K562E was lower than that by dynamin 2-WT. Dynamin 2-WT partially colocalized with stress fibers and actin bundles based on double immunofluorescence of human podocytes. Dynamin 2-K562E expression resulted in decreased stress fiber density and the formation of aberrant actin clusters. Dynamin 2-K562E colocalized with alpha-actinin-4 in aberrant actin clusters. Reformation of stress fibers after cytochalasin D-induced actin depolymerization and washout was less effective in dynamin 2-K562E-expressing cells than that in dynamin 2-WT. Bis-T-23, a dynamin self-assembly enhancer, was unable to rescue the decreased focal adhesion numbers and reduced stress fiber density induced by dynamin 2-K562E expression. These results suggest that the low affinity of the K562E mutant for lipid membranes, and atypical self-assembling properties, lead to actin disorganization in HPCs. Moreover, lipid-binding and self-assembly of dynamin 2 along actin filaments are required for podocyte morphology and functions. Finally, dynamin 2-mediated interactions between actin and membranes are critical for actin bundle formation in HPCs. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 Biochemical characterization of the insertase Alb3.1 involved in the assembly of light-harvesting chlorophyll a/b complexes EN MITHUN KUMAR RATHOD Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 Geochemical study of Late Cenozoic mafic volcanic rocks from Vietnam: Implications for genesis of intraplate volcanism and mantle heterogeneity in Southeast Asia EN DAO VAN NGHIEM Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 111 2022 糸状菌Trichoderma viride由来の抗腫瘍性酵素L-リシン α-オキシダーゼに関する研究 7 14 EN Masaya Saito Course of Agrochemical Bioscience Kenji Inagaki L-Lysine α-oxidase (LysOX) from Trichoderma viride is a homodimeric flavoenzyme that catalyzes the oxidative deamination of L-Lysine to produce α-keto-ε-aminocaproate with ammonia and hydrogen per-oxide. LysOX inhibited the growth of cancer cells but showed relatively low toxicity for normal cells. The full-length cDNA consists of 2,119 bp, and encodes a long N-terminal propeptide composed of 77 resi-dues (Met1-Arg77) and the mature protein (Ala78-Ile617). The LysOX gene was heterologously expressed in Streptomyces lividans TK24 or Escherichia coli SoluBL21. The enzymatic properties of the purified recombinant LysOX, such as substrate specificity, kinetic parameters and thermal stability, are the same as those of the native LysOX. The LysOX precursor (prLysOX) expressed in E. coli shows weak enzymatic activity and is activated by proteolytic processing. The crystal structure of prLysOX revealed that the propeptide of prLysOX indirectly changes the active site structure to inhibit enzyme activity. Moreover, the crystal structures of LysOX and its L-Lysine complex revealed that the hydrogen bonding network formed by Asp212, Asp315 and Ala440 with two water molecules is responsible for the recogni-tion of the ε-amino group of L-Lysine. In addition, a narrow substrate-binding site and acidic surface at the active site entrance both contribute to strict substrate specificity. Mutational analysis demonstrated that Asp212 and Asp315 are essential for substrate recognition, and the D212A/D315A LysOX prefers aromatic amino acids. Furthermore, the structural basis of the substrate specificity change has also been revealed by the structural analysis of the D212A/D315A LysOX and its substrate complexes. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Histopathological and Immunohistochemical Studies on The Breast Cancer Stem Cells Developed in The Microenvironment of Mouse Mammary Fat Pads EN HAGAR ALI ABDUL RAHEEM ABU QUORA Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Nanog is a promising chemoresistant stemness marker and therapeutic target by iron chelators for esophageal cancer EN Toru Narusaka Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Oncolytic virotherapy reverses chemoresistance in osteosarcoma by suppressing MDR1 expression EN Kazuhisa Sugiu Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Pharmaceutical Society of Japan Acta Medica Okayama 0031-6903 141 10 2021 光受容タンパク質・ロドプシンの生物物理化学研究 1155 1160 EN Keiichi Kojima Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Opsins (also called animal rhodopsins) are universal photoreceptive proteins that provide the molecular basis of visual and nonvisual photoreception in animals, including humans. Opsins consist of seven helical a-transmembrane domains and use retinal, a derivative of vitamin A, as a chromophore. In many opsins, light absorption triggers photoisomerization from 11-cis retinal to all-trans retinal, resulting in activation via dynamic structural changes in the protein moiety. Activated opsins stimulate cognate trimeric G proteins to induce signal transduction cascades in cells. Recently, molecular and physiological analyses of diverse opsins have progressively advanced. This review introduces the molecular basis and physiological unctions of opsins. Based on the functions of opsins, I will discuss the potential of opsins as target molecules to treat and prevent visual and nonvisual diseases such as sleep disorder and depression. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Robust functionalization of graphene for organic electrode materials EN KHAN RIZWAN Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 5-aminolevulinic acid-mediated photodynamic therapy can target aggressive adult T cell leukemia/lymphoma resistant to conventional chemotherapy EN Yasuhisa Sando Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 11 2021 工業の免許状取得の必修科目「工業概論」「工業科教育法」「職業指導概説」で取り扱う指導内容―工業系高校の学科構成と高等学校学習指導要領を踏まえて― 225 236 EN Seitaro KOBAYASHI Center for Teacher Education and Development, Okayama University 10.18926/CTED/61576 高等学校の工業系学科は地域の産業界の要請に応えて設置され，これまで多くの実践的な中堅技術者を育成し輩出してきた。教育には地域産業に関連した内容が積極的に取り入れられており，機械系・電気系・化学系・建築系・土木系・情報系等の専門分野に分かれている。このようなことから，工業系学科の教員には多様な専門分野の人材が必要とされる。本著では，工業系学科の教員に求められる知識・技術を明確にするとともに，「工業概論」「工業科教育法（基礎�T，基礎�U，応用�T，応用�U）」「職業指導概説」で確実に押さえておくべき指導内容について述べる。 No potential conflict of interest relevant to this article was reported.

Japan Society for Cell Biology Acta Medica Okayama 0386-7196 45 2 2020 Internalization of AMPA-type Glutamate Receptor in the MIN6 Pancreatic β-cell Line 121 130 EN The Mon La Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hiroshi Yamada Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Sayaka Seiriki Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Shun-AI Li Center for Innovative Clinical Medicine, Okayama University Hospital Kenshiro Fujise Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Natsuho Katsumi Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Tadashi Abe Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Masami Watanabe Center for Innovative Clinical Medicine, Okayama University Hospital Kohji Takei Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences The activity of AMPA-type glutamate receptor is involved in insulin release from pancreatic β-cells. However, the mechanism and dynamics that underlie AMPA receptor-mediated insulin release in β-cells is largely unknown. Here, we show that AMPA induces internalization of glutamate receptor 2/3 (GluR2/3), AMPA receptor subtype, in the mouse β-cell line MIN6. Immunofluorescence experiments showed that GluR2/3 appeared as fine dots that were distributed throughout MIN6 cells. Intracellular GluR2/3 co-localized with AP2 and clathrin, markers for clathrin-coated pits and vesicles. Immunoelectron microscopy revealed that GluR2/3 was also localized at plasma membrane. Surface biotinylation and immunofluorescence measurements showed that addition of AMPA caused an approximate 1.8-fold increase in GluR2/3 internalization under low-glucose conditions. Furthermore, internalized GluR2 largely co-localized with EEA1, an early endosome marker. In addition, GluR2/3 co-immunoprecipitated with cortactin, a F-actin binding protein. Depletion of cortactin by RNAi in MIN6 cells altered the intracellular distribution of GluR2/3, suggesting that cortactin is involved in internalization of GluR2/3 in MIN6 cells. Taken together, our results suggest that pancreatic β-cells adjust the amount of AMPA-type GluR2/3 on the cell surface to regulate the receptive capability of the cell for glutamate. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 Electrochemical synthesis and functionalization of graphene materials for energy storage devices EN Benoît Denis Louis Campéon No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 Isolation, characterization and structural analysis of tetrameric photosystem I from a filamentous N2 fixing cyanobacterium Anabaena sp. PCC 7120 EN Tianyi Jiang Graduate School of Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

Wiley Acta Medica Okayama 0892-6638 34 12 2020 Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes 16449 16463 EN The Mon La Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hiromi Tachibana Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Shun-Ai Li Center for Innovative Clinical Medicine, Okayama University Hospital Tadashi Abe Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Sayaka Seiriki Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hikaru Nagaoka Division of Malaria Research, Proteo-Science Center, Ehime University Eizo Takashima Division of Malaria Research, Proteo-Science Center, Ehime University Tetsuya Takeda Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Daisuke Ogawa Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Shin-Ichi Makino Department of Nephrology, Graduate School of Medicine, Chiba University Katsuhiko Asanuma Department of Nephrology, Graduate School of Medicine, Chiba University Masami Watanabe Center for Innovative Clinical Medicine, Okayama University Hospital Xuefei Tian Department of Internal Medicine, Section of Nephrology, Yale University School of Medicine Shuta Ishibe Department of Internal Medicine, Section of Nephrology, Yale University School of Medicine Ayuko Sakane Department of Biochemistry, Tokushima University Graduate School of Medical Sciences Takuya Sasaki Department of Biochemistry, Tokushima University Graduate School of Medical Sciences Jun Wada Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kohji Takei Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hiroshi Yamada Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Dynamin 1 is a neuronal endocytic protein that participates in vesicle formation by scission of invaginated membranes. Dynamin 1 is also expressed in the kidney; however, its physiological significance to this organ remains unknown. Here, we show that dynamin 1 is crucial for microtubule organization and stabilization in glomerular podocytes. By immunofluorescence and immunoelectron microscopy, dynamin 1 was concentrated at microtubules at primary processes in rat podocytes. By immunofluorescence of differentiated mouse podocytes (MPCs), dynamin 1 was often colocalized with microtubule bundles, which radially arranged toward periphery of expanded podocyte. In dynamin 1-depleted MPCs by RNAi, alpha-tubulin showed a dispersed linear filament-like localization, and microtubule bundles were rarely observed. Furthermore, dynamin 1 depletion resulted in the formation of discontinuous, short acetylated alpha-tubulin fragments, and the decrease of microtubule-rich protrusions. Dynamins 1 and 2 double-knockout podocytes showed dispersed acetylated alpha-tubulin and rare protrusions. In vitro, dynamin 1 polymerized around microtubules and cross-linked them into bundles, and increased their resistance to the disassembly-inducing reagents Ca(2+)and podophyllotoxin. In addition, overexpression and depletion of dynamin 1 in MPCs increased and decreased the nocodazole resistance of microtubules, respectively. These results suggest that dynamin 1 supports the microtubule bundle formation and participates in the stabilization of microtubules. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 The Influence of Chemotherapy-Induced Peripheral Neuropathy on Quality of Life of Gynecologic Cancer Survivors EN Hirofumi Matsuoka Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学文学部国語国文学研究室 Acta Medica Okayama 0386-3123 10 1982 『連環記』論――「連環」の形態と世界への展開―― 103 115 EN 10.18926/okadaironkou/60665 No potential conflict of interest relevant to this article was reported.

岡山大学文学部国語国文学研究室 Acta Medica Okayama 0386-3123 12 1984 Mori Ogai : Arzt und Dichter -- die bedeutung seines Deutschlandaufenthalts -- 216 200 EN Masako Hayashi 10.18926/okadaironkou/60593 No potential conflict of interest relevant to this article was reported.

岡山大学文学部日本語日本文学研究室 Acta Medica Okayama 0386-3123 30 2002 六条御息所考 29 36 EN 10.18926/okadaironkou/60164 No potential conflict of interest relevant to this article was reported.

岡山大学文学部言語国語国文学会 Acta Medica Okayama 0386-3123 31 2003 談話における「いや」の用法 129 120 EN 10.18926/okadaironkou/60147 No potential conflict of interest relevant to this article was reported.

岡山大学文学部言語国語国文学会 Acta Medica Okayama 0386-3123 32 2004 「赤い色」と「赤い着物」――本文の生成をめぐって―― 61 70 EN 10.18926/okadaironkou/60123 No potential conflict of interest relevant to this article was reported.

岡山大学文学部言語国語国文学会 Acta Medica Okayama 0386-3123 34 2006 日本語定型詩歌のリズム―「等時音律説」再論― 51 59 EN 10.18926/okadaironkou/60095 No potential conflict of interest relevant to this article was reported.

岡山大学文学部言語国語国文学会 Acta Medica Okayama 0386-3123 35 2007 日本詩のリズムと単位音数―明治二十年代の諸論から― 36 44 EN 10.18926/okadaironkou/60085 No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 Evaluation of Therapeutic Target Gene Expression Based on Residual Cancer Burden Classification After Neoadjuvant Chemotherapy for HER2-Negative Breast Cancer EN Yuko Takahashi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 131 3 2019 薬物相互作用（46―注射薬の配合変化 : 注射薬の物理的・化学的な相互作用） 161 164 EN Yosuke Morishita Department of Pharmacy, Okayama University Hospital Satoru Esumi Department of Pharmacy, Okayama University Hospital Shigeki Nishihara Department of Pharmacy, Okayama University Hospital Yoshihisa Kitamura Department of Pharmacy, Okayama University Hospital Toshiaki Sendo Department of Pharmacy, Okayama University Hospital No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 131 3 2019 平成30年度岡山医学会賞 脳神経研究奨励賞（新見賞） 135 137 EN Midori Edamatsu Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 Clinical and biochemical characteristics of patients having general symptoms with increased serum IgG4 EN Ko Hasegawa Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

GEORG THIEME Acta Medica Okayama 09365214 30 10 2019 1,10-Phenanthroline- or Electron-Promoted Cyanation of Aryl Iodides 1209 1214 EN Koichi Mitsudo Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Kazuki Yoshioka Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Takayuki Hirata Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Hiroki Mandai Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Koji Midorikawa Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Seiji Suga Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University A 1,10-phenanthroline-promoted cyanation of aryl iodides has been developed. 1,10-Phenanthroline worked as an organocatalyst for the reaction of aryl iodides with tetraalkylammonium cyanide to afford aryl cyanides. A similar reaction occurred through an electroreductive process. No potential conflict of interest relevant to this article was reported.

American Chemical Society Acta Medica Okayama 15237060 21 7 2019 Iodide-Mediated or Iodide-Catalyzed Demethylation and Friedel-Crafts C-H Borylative Cyclization Leading to Thiophene-Fused 1,2-Oxaborine Derivatives 2171 2175 EN Keisuke Shigemori Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Momoka Watanabe Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Julie Kong Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Koichi Mitsudo Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Atsushi Wakamiya Institute for Chemical Research, Kyoto University Hiroki Mandai Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Seiji Suga Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University The first synthesis of dithieno-1,2-oxaborine derivatives was achieved via iodide-mediated or iodide-catalyzed demethylation of 3-methoxy-2,2'-bithiophene and subsequent C-H borylation. A wide variety of thiophene-fused oxaborines could be synthesized by the procedure. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 ラットを用いた化学物質曝露による生体内ホルモンレベルの変動要因解析 EN Takafumi Yamaguchi Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 The influence of physicochemical parameters on photochemical internalization (PCI) mechanism and application of PCI as a photo-dependent RNA delivery strategy to mouse embryo EN TET HTUT SOE Graduate School of Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 The epithelial-to-mesenchymal transition induced by tumor-associated macrophages confers chemoresistance in peritoneally disseminated pancreatic cancer EN Kazuya Kuwada Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 131 1 2019 平成29年度岡山医学会賞 総合研究奨励賞（結城賞） 13 15 EN Tetsuya Takeda Department of Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 35 2019 新生児マウスを用いる動物実験が母マウスにもたらすストレスの生化学的評価 22 25 EN Norio Yata Advanced Science Research Center, Okayama University Corticosterone, the glucocorticoid in rodents, is released from adrenal cortex in response to stress. Sometimes, for use in experiments, infants may be separated from their mother mouse. We studied the stress of mother mouse when she separates from infants. As a result, the concentration of corticosterone in the blood of the mother mouse tended to increase, by pulling her infants apart. In the case of using infant mice for animal experiments, it was suggested that it is necessary to consider the maternal stress. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 Structural and functional studies of oxygen-evolving photosystem II EN Yoshiki Nakajima Graduate School of Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 Changes in Serum Biochemical Markers in Relation to Chief Complaints and Aging in General Medicine EN Daisuke Omura Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 The Glasgow Prognostic Score Determined During Concurrent Chemoradiotherapy Is an Independent Predictor of Survival for Cervical Cancer EN Takeshi Nishida Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 Detection of Circulating MicroRNAs with Ago2 Complexes to Monitor the Tumor Dynamics of Colorectal Cancer Patients during Chemotherapy EN Tomokazu Fuji Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 130 3 2018 20世紀初頭に培養細胞に関心を示した 2人のノーベル賞受賞者 : Alexis Carrelと Peyton Rous 129 133 EN Masayoshi Namba Okayama Medical Foundation 21世紀になって2017年までに，34件のノーベル生理学・医学賞と化学賞が出ているが，その約半数の研究に培養細胞（動物の細胞，あるいは，イースト）が使われている．すなわち，現代の生命科学の研究には，培養細胞がきわめて大きな役割を果たしているといえる．今回は，今から約100年前，細胞を培養して研究を進めようとした先見性に富んだ 2人のノーベル生理学・医学賞受賞者，Alexis Carrel（以下，本文ではカレルとする）と Peyton Rous（以下，ラウス）の経歴と業績を紹介し，彼等の培養細胞との係りについて述べる． No potential conflict of interest relevant to this article was reported.

岡山大学法学会 Acta Medica Okayama 0386-3050 68 3-4 2019 事実婚・内縁の死亡解消における居住権と夫婦財産制 : 2018年相続法改正による生存配偶者の居住権の新設を契機に 634 599 EN Y. Furukawa No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 9 2019 「またやるのですか？」からの出発 : 岡山県教委の不祥事対策の3年 13 22 EN Chiaki Tsukamoto Shinji Hira 10.18926/CTED/56537 　岡山県教育委員会が取り組んできた不祥事対策について，その特徴を回顧的に述べた上で，3年が経過した現在の成果について紹介した。ともすると不祥事対策は「言われるからやりました」というアリバイ作り的なものになりやすく，研修の場での発言も形式的なものに傾きやすい。そうした動機づけの低さを「多忙な教員として自然な反応」と認識したうえで，生徒理解を超え，教師自身の人生理解に役に立つように研修資料を作りこんできた結果，現場では「研修を受けてよかった」「研修を繰り返すことに意味があると思える」という声も聞かれるようになった No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Dose-Volume Parameters Predict Radiation Pneumonitis after Surgery with Induction Concurrent Chemoradiotherapy for Non-small Cell Lung Cancer EN Takeshi Ogata Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 108 2019 B（ホウ素）欠乏処理がイチゴのチップバーンおよび受精不良果発生に及ぼす影響 5 13 EN Miho Sesumi Graduate school of Environmental and Life Science Yuichi Yoshida Graduate school of Environmental and Life Science Akari Kinjo Graduate school of Environmental and Life Science Kei Hidaka Asahi Kagaku Kogyo Co., Ltd.) Tanjuro Goto Graduate school of Environmental and Life Science Ken-ichiro Yasuba Graduate school of Environmental and Life Science Yoshiyuki Tanaka Graduate school of Environmental and Life Science Boron (B) is an essential micro element for plants and plays important roles in the synthesis and functions of cell wall. B deficiency has been reported as one of the causes of fruit malformation in strawberries. We investigated the effect of B deficiency on flower and fruit development of forced strawberries for two cropping seasons (2015-2017). In the second season, B was resupplied for B-deficient plants and we investigated changes in fruit development. When B-free nutrient solutions were supplied, tip burn began to occur in newly emerging leaves and calyx 2 to 3 months later, and fruit malformation including seedy or only partly developed fruits with undeveloped achenes occurred frequently. However, these deficient symptoms were quickly disappeared by supplying B containing nutrient solutions. In conclusion, B nutrition is closely related to the occurrence of fruit malformation through fertility of pollen and pistils, and also development of receptacle tissue in strawberries. It should be possible to reduce fruit malformation in strawberries by proper control of B nutrition. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Geochemical studies of inorganic nitrogen source for chemosynthesis-based animals EN Yuji Onishi Graduate School of Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学文学部言語文化学科ヨーロッパ言語文化論講座 Acta Medica Okayama 13462725 37 2018 フリードリヒ・シンケルの建築と美術史 : ベルリン旧博物館における絵画の展示をめぐって 21 35 EN Mao Mitsui Dieser Aufsatz behandelt die Anordnung der Gemälde im Alten Museum Berlin bei seiner Eröffnung im Jahr 1830. Damals vertraten die Teilnehmer der Kommission zur Einrichtung des Museums sehr unterschiedliche Meinungen über die mögliche Aufhängung der Exponate. Das Alte Museum Berlin war eines der ersten Museen in Deutschland und es gab damals noch keine grundsätzlichen Empfehlungen oder Vorgaben zur Anordnung von Kunstwerken. In diesem Zusammenhang sind bisher vor allem die Bemerkungen des Vorsitzenden der Kommission, Wilhelm von Humboldt, mit Blick auf seine Sprachphilosophie untersucht worden. Der Schwerpunkt der folgenden Betrachtung liegt auf der von dem Architekten des Museums, Karl Friedrich Schinkel, vertretenen Auffassung zur Anordnung der Ausstellungsstücke. Dabei soll nicht nur die Kunstanschauung Schinkels selbst, sondern auch die Bedeutung des Museums als moderne Institution Berlins berücksichtigt werden. Einer Vorlesung des Archäologen Aloys Hirt im Jahr 1797 ist zu entnehmen, dass er das Museum besonders für Künstler und Wissenschaftler einrichten wollte. Er betrachtete ein Museum als ein Institut, das dazu dienen sollte, den Geschmack von Künstlern und Fachleuten auszubilden und die Industrie Preußens zu entwickeln. Dagegen hielten Schinkel und der Kunsthistoriker Friedrich Waagen, deren Vorschläge erst 1828 geäußert wurden, es vielmehr für wichtig, dem „Publikum“ Kenntnisse über Kunstgeschichte zu vermitteln. Dem Entwurf von Schinkel und Waagen fügten Humboldt und der Kunsthistoriker Carl Friedrich von Rumohr ein Gutachten über ästhetische Aspekte hinzu. Obwohl Humboldts und Rumohrs Vorschläge für die Aufhängung der Gemälde sich von denen Schinkels und Waagens unterschieden, spielte Schinkels Architektur des Museumsgebäudes eine wichtige Rolle dabei, die Ziele beider Seiten zu verwirklichen. Der Aufsatz kommt zu dem Schluss, dass das Alte Museum für Schinkel nicht nur Ausdruck seiner Verehrung für die Antike war, wie oft gesagt wird, sondern auch ein Mittel darstellte, das Publikum des 19. Jahrhunderts durch kunstgeschichtliche Kenntnisse mit der Antike zu verbinden. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 130 1 2018 症例報告 乳腺化生癌の2例 19 23 EN Takayuki Motoki Department of Surgery, Okayama Saiseikai General Hospital Takayuki Iwamoto Center for Innovative Clinical Medicine, Okayama University Hospital Masako Omori Department of Diagnostic Pathology, Kurashiki Medical Center Junji Matsuoka Department of Palliative and Supportive Medicine, Okayama University Hospital We treated two patients with a rare metaplastic carcinoma of the breast. Patient 1：A 32-year-old woman presented with a rapidly growing mass（ 7cm） in her right breast. We diagnosed cT3N0M0 Stage IIB breast cancer and performed a radical resection. The pathological diagnosis was triple-negative metaplastic carcinoma with a Ki-67 value ＞30%. Postoperative adjuvant chemotherapy and radiotherapy were performed. Patient 2：A 66-year-old postmenopausal woman presented with a left breast mass. We diagnosed cT1N0M0 Stage I breast cancer and performed a radical resection. The pathological diagnosis was triple-negative metaplastic carcinoma with a Ki-67 value ＞30%. Postoperative adjuvant chemotherapy was performed. Patients 1 and 2 have achieved relapse-free survivals of 1 year＋2 months and 10 months, respectively. Metaplastic carcinoma of the breast is a rare tumor, and its prognosis is not favorable. The only available treatment is that used for invasive ductal carcinoma；a more specific treatment has not been established. The accumulation of further similar cases and the development of a novel effective treatment are desired. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 130 1 2018 非小細胞肺癌に対する術後補助化学療法 9 12 EN Shinichi Toyooka Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Petrologic and geochemical evidence for diversity of magma compositions at the Ethiopian volcanic province: implications for thermo-chemical plume influence EN Graduate School of Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Petrological, geochronological and geochemical study of late Cenozoic volcanic rocks from the Chugoku district, southwest Japan: Implications for the evolution of subduction zone and volcanic arc EN Graduate School of Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Tumour-infiltrating lymphocytes (TILs)-related genomic signature predicts chemotherapy response in breast cancer EN Mariko Kochi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学大学院文化科学研究科 Acta Medica Okayama 1880-9162 17 2018 清末における羅振玉の日本視察と訪書活動 19 32 EN Shunchang Lu 10.18926/55793 No potential conflict of interest relevant to this article was reported.

岡山大学全学教育・ 学生支援機構 Acta Medica Okayama 2432-9665 2 2017 『論語集注』（朱熹撰）の日本語訳（學而第一） : 『論語集注』を主とする朱子の『論語』解釈 87 106 EN Luyi Sun Institute for Education and Student Services, Okayama University 10.18926/55719 　周知の通り、朱憙（一一三○〜一二○○。朱子は尊称）の『論語』解釈は、中国思想の発展に寄与しただけではなく、日本や朝鮮半島などの東アジアの思想の発展にも大きな影響を与えたものである。だが、『論語』には「道」「心」「徳」「君子」などの中国哲学の概念が随所に現れており、朱子哲学においてのそれらの概念の含意を明確に解明しない限り、朱子の『論語』解釈の全内容を理解することは極めて難しいと思われるのである。筆者は、長年に渡って朱子哲学の研究に力を注ぎ、いままでは既に、「朱子の「太極」と「気」（岡山大学『大学教育研究紀要』第七号、二○一一年）「朱子の「神」」（岡山大学『大学教育研究紀要』第八号、二○一二年）「朱子の「心」」（京都大学『中國思想史研究』第三十四號、二○一三年）「朱子の「理」」（岡山大学『大学教育研究紀要』第十号、二○一四年）「朱子の「情」」（岡山大学『大学教育研究紀要』第十一号、二○一五年）「朱子の「変化気質」」（『岡山大学大学院社会文化学科研究科紀要』第四三号、二○一七年）「朱子の「君子」」（『岡山大学大学院社会文化学科研究科紀要』第四四号、二○一七年）などの論文を発表した。その朱子哲学の研究を通じて、筆者は、上記の諸論文、及び『四書章句集注』（新編諸子集成、中華書局、一九八三年）と『朱子語類』（全八冊、宋・黎靖徳編、王星賢点校、一九九四年）、特に『朱子語類』に所収の「論語（一〜三十二）」（巻第十九〜五十）に基づいての、『論語集注』を主とする朱子の『論語』解釈の現代日本語の完全翻訳を作成することが必要だと強く思うようになったのである。本稿では、『論語集注』（前掲の『四書章句集注』に所収）の「學而第一」の朱子の集注を和訳することと、『論語』學而第一の原文を主に『朱子語類』（前掲）に所収の「論語（一〜三十二）」に記録されている朱子の説明に基づいて和訳することを試 みる。 No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Geochemical Study of the Cameroon Volcanic Line: Implication for the Genesis of Passive Margin Intraplate Magmatism EN Iyasu Getachew Belay Graduate School of Natural Science and Tchnology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Immunohistochemistry of Vasohibin-2 in Human Kidney Disease: Implication in Impaired Glucose Tolerance and Reduced Renal Function EN Yuka Arata Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 BRAF V600E mutation is a predictive indicator of upfront chemotherapy for stage IV colorectal cancer EN Tatsuya Morikawa Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 マウスモデルを用いた大気中の化学物質により引き起こされる吸入アレルギーの病態解明に関する研究 EN Risako Tajiki Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 低分子医薬品を指向したペプチドミメティクスの工業的化学合成法の開発研究 EN Yasuhiro Sawai Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Geochemical and Geochronological Study of the Cenozoic Adakitic Lavas from SW Japan: Implications for the Slab-Mantle Interactions in a Hot Subduction System EN Ivan Pineda Velasco Graduate School of Natural Science and Tchnology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 抗糖尿病作用を有する亜鉛錯体の生体内挙動の可視化と生体内化学形態変化の解析 EN Masayuki Munekane Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 The Immunological Impact of Chemotherapy on the Tumor Microenvironment of Oral Squamous Cell Carcinoma EN Hiroaki Takakura Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 7 2017 情緒の安定に課題のある自閉症児への自己理解に基づく自立活動 : 自己制御機能に着目した指導記録の分析 61 70 EN The University of Hiroshima Bunka Gakuen Akitaka Nakaya Graduate School of Education,Okayama University 10.18926/CTED/54931 No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 7 2017 中学校の道徳教育において〈いのち〉の教育を どのように実践するか（２） 11 20 EN Michiru Watanabe Hiroshima Bunka Gakuen University Mariko Kokayashi Graduate School of Education, Okayama University 10.18926/CTED/54926 　阪神・淡路大震災と東日本大震災では，大人だけでなく，多くの子どもたちが犠牲となった。改めて｢命の教育｣の重要性が指摘されている。また，青少年，特に中学生による殺傷事件やいじめによる自殺も後を絶たない現状にある。一方，2019（平成31）年度から中学校の「道徳の時間」は「特別の教科􀀃 道徳」という名称の教科となる。その理由は様々であるが，教育再生実行会議の第一次提言によれば，同会議が教科化提言に踏み切った直接の要因は，いじめ問題への実効性のある道徳教育を求めることにあった。しかし，学校の道徳教育や「命の教育」は役に立たないという指摘もあり，教科となった道徳科における道徳教育は，これまでの諸課題を確実に見直し，確たる実践的基盤を打ち立てなければならない。本稿（２）では，前号の（１）で論じた〈いのち〉とその教育に関する独自の観点によりながら，「〈いのち〉の教育」に取り組む「道徳科の授業」の新しい展開を提案したい。 No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 129 1 2017 抗菌化学療法で保存的に閉鎖した放線菌症による 難治性皮膚直腸膀胱瘻の1例 35 39 EN Yuki Katsura Department of Surgery, Hiroshima City Hiroshima Citizens Hospital Hiroyoshi Matsukawa Department of Surgery, Hiroshima City Hiroshima Citizens Hospital Takuya Kato Department of Surgery, Hiroshima City Hiroshima Citizens Hospital Masahiro Sugihara Department of Surgery, Hiroshima City Hiroshima Citizens Hospital Yasutomo Ojima Department of Surgery, Hiroshima City Hiroshima Citizens Hospital Shigehiro Shiozaki Department of Surgery, Hiroshima City Hiroshima Citizens Hospital The patient was a 35-year-old Japanese man diagnosed with appendicitis with abscess formation. An appendectomy was performed, but a refractory surgical wound infection developed, and eventually a cutaneous-rectovesical fistula was detected. In a review of the first-time CT scan, a small high-density construction resembling a bone from a fish was detected in the ileum. The histopathological examination revealed granules of actinomyces. These findings suggested that abdominal actinomycosis due to intestinal mucosal breakage by the fish bone caused the secondary appendicitis, and that after the appendectomy, residual actinomyces caused the cutaneous-rectovesical fistula. After the diagnosis, total parenteral nutrition and a long-term administration of antibiotics improved the patient's clinical symptoms, and the fistula closed within a month. Antibiotics were administered for 6 months, and there has been no recurrence for 6-1/2 years. Because actinomycosis is difficult to diagnose based on the typical clinical features, a direct identification of the infecting organism from a tissue sample or from sulfur granules is required for the definitive diagnosis. Actinomyces is also known to cause fistula formation, and intestinal penetration caused by a fish bone may indicate abdominal actinomycosis. A rectovesical fistula requires surgical intervention in most cases, but in cases caused by abdominal actinomycosis, such a fistula may be cured by conservative therapy, as in our patient's case. It is important to consider the possibility of actinomycosis when a refractory rectovesical fistula is observed. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 Ablation of MCL1 expression by virally induced microRNA-29 reverses chemoresistance in human osteosarcomas EN Shuhei Osaki No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 106 2017 Ginkgo biloba α-fucosidase with activity towards plant complex type N-glycans containing the Lewis a epitope: Purification and characterization 5 12 EN Satsuki Itano Graduate School of Environmental and life Science, Okayama University Megumi Maeda Graduate School of Environmental and life Science, Okayama University Md. Ziaur Rahman Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission Yoshinobu Kimura Graduate School of Environmental and life Science, Okayama University 　We have identified, and purified to homogeneity, a high molecular weight Ginkgo biloba α-fucosidase (α-fucosidase Gb, 120 kDa estimated by SDS‒PAGE) with activity against α-fucosylated oligosaccharides. When a Lewis a epitope-containing N-glycan was used as a substrate, α-fucosidase Gb showed optimum activity at approximately pH 5.5, suggesting that it functions in acidic environments such as the vacuole. It remains uncertain, however, whether this Ginkgo α-fucosidase belongs to the GH29 family, since its N-terminal sequence could not be determined, probably due to a chemical modification. α-Fucosidase Gb showed substantial activity towards the α1,3-fucosyl linkage in Lacto-N-fucopentaose III and an α1,4-fucosyl linkage in the Lewis a epitope found in plant complex type N-glycans, indicating an involvement in the degradation process of α-fucosylated oligosaccharides or N-glycoproteins. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 電気化学的呼吸量測定による豚胚の客観的評価と胚移植による種豚生産システムの開発に関する研究 EN Nobutada Sakagami No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 マイクロ波表面波プラズマ化学気相成長法を用いたグラフェンおよびアモルファスカーボン膜の成長と物性評価 EN Susumu Ichimura No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 128 3 2016 集学的治療により長期生存が得られた食道神経内分泌癌の2 例 207 212 EN Tatsuhiro Gotoda Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Seiji Kawano Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Yoshiyasu Kono Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Kou Miura Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Hiromitsu Kanzaki Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Masaya Iwamuro Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Yoshiro Kawahara Department of Endoscopy, Okayama University Hospital Takehiro Tanaka Department of Pathology, , Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Tadashi Yoshino Department of Pathology, , Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Yasuhiro Shirakawad Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Masahiro Tabata Department of Hematology and Oncology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Mitsune Tanimoto Department of Hematology and Oncology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Hiroyuki Okada Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences 　Esophageal neuroendocrine carcinoma （ECC） is rare and has a poor prognosis when presenting with vascular invasion and distant metastasis from an early stage. Multidisciplinary therapy with surgery, chemotherapy, and radiation therapy may prolong survival in patients with advanced ECC, but there is as yet no standard therapy for advanced ECC. We treated two patients who have achieved long-term survival （＞ 4 years） who underwent multidisciplinary therapy, including chemotherapy, for ECC. Our experience with these two cases suggests that multidisciplinary therapy, including chemotherapy, may be effective for treating ECC at an advanced stage. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 128 2 2016 腸骨広範囲切除術後に発生した腹壁瘢痕ヘルニアに対しメッシュ修復術を行った一例 117 120 EN Kazunori Tsukuda Minimally Invasive Therapy Center, Okayama University Hospital Hiroaki Asano Minimally Invasive Therapy Center, Okayama University Hospital Yasuhiro Mandai Center of the Development and Health Care Education, Okayama University Toshiyoshi Fujiwara Department of Gastrointestinal Sugery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences 　The patient was a 46-year old Japanese female who had undergone wide excision of the iliac bone and hip transposition at our institute's orthopedics department 2 years earlier. She presented with a growing incisional hernia and was transferred to our gastroenterological surgery department for surgical treatment. We planned a mesh repair for the incisional hernia, which protruded over the right iliac bone. The dimensions of the abdominal defect were 15×9 cm, and we used prolene mesh to repair the defect. The mesh was fixed at the inner part of the iliac bone, folded back at the iliac horn and fixed to the abdominal oblique muscles. The postoperative course was smooth, and recurrence was not seen at 3.5 years after the operation. An incisional hernia as seen in this patient's case is very rare, but we found that the underlay technique and prolene mesh were very useful for the three-dimensional hernia repair. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 スパッタ法により作製されたナノアイランド構造を有する非晶質酸化ニオブの光電気化学特性と構造解析 EN Go Sajiki No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 Synthesis and Physicochemical Properties of Substituted Picenes EN Xi-chao Chen No potential conflict of interest relevant to this article was reported.

岡山大学大学院文化科学研究科 Acta Medica Okayama 1880-9162 15 2016 ムスリム文化学習の教材構成に向けた予備調査： 日本人学生を対象とした文化アシミレーター試作版への反応 131 153 EN Sachiko Nakano Tomoko Tanaka 10.18926/54221 No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 128 1 2016 原発性十二指腸粘液癌の一例― 本邦報告16例の検討― 21 25 EN Ikumi Hamano Yusuke Matsumoto Yoshikatsu Endo Naoki Watanabe Kyouhei Kai Shizou Sato Yoji Wani 　Primary mucinous adenocarcinoma of the duodenum is rare. Here we report a case we recently encountered, and we review 16 cases reported in Japan. An 82-year-old Japanese woman was admitted to our hospital complaining of abdominal pain and heartburn. An endoscopic examination revealed a Type 2 tumor in the descending limb of the duodenum, and endoscopically obtained specimens revealed a poorly differentiated adenocarcinoma. We performed a curative pancreatoduodenectomy with lymph node resection, and the surgical specimen revealed that the duodenum was the primary site of the mucinous adenocarcinoma. The patient is currently alive ＞ 1 year after the operation without any evidence of recurrence. Of the 16 patients reviewed, all patients had advanced tumors those depth were T3-T4. 9 patients had lymph node metastasis and 4 patients had peritoneal dissemination at the time of surgery. Since mucinous adenocarcinoma of the duodenum is often progressive cancer at a diagnosis, which is tend to have a worse prognosis than other histological types. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 Neoadjuvant Chemotherapy with or without Concurrent Hormone Therapy in Estrogen Receptor -Positive Breast Cancer: NACED-Randomized Multicenter Phase �U Trial EN Kumi Sugiu No potential conflict of interest relevant to this article was reported.

岡山大学文学部言語文化学科ヨーロッパ言語文化論講座 Acta Medica Okayama 1346-2725 22 2003 「人間は滅ぶものである……」 -『オーベルマン』第90信の一筋について― 49 62 EN Naoyuki Hagiwara No potential conflict of interest relevant to this article was reported.

岡山大学文学部言語文化学科ヨーロッパ言語文化論講座 Acta Medica Okayama 1346-2725 21 2002 メテルヴィルの葡萄摘み ―「オーベルマン」第9信の受容について― 75 92 EN Naoyuki Hagiwara No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 127 3 2015 術前内照射および化学療法が著効し,根治切除し得た巨大異所性褐色細胞腫の1例 213 218 EN Kazuya Yasui Yuzo Umeda Kenjiro Kumano Masahiro Tabata Fumio Otsuka Takahito Yagi Toshiyoshi Fujiwara A 46-year-old man was found to be positive for occult blood at a medical checkup and was revealed to have a 14-cm tumor on the right side of abdominal aorta by a subsequent abdominal CT scan. The endocrinology laboratory data showed elevations in the levels of serum noradrenaline, and ectopic pheochromocytoma was suspected. The tumor was compressing the inferior vena cava and portal vein, the superior mesenteric artery and the pancreas. Since it would be difficult to cure by operation, neoadjuvant therapy was started using radioisotope therapy by I-131 metaiodobenzylguanidine (131I-MIBG) and chemotherapy (CVD therapy ; cyclophosphamide, vincristine, dacarbazine). He was treated with three courses of radioisotope therapy and 16 courses of chemotherapy, which significantly reduced the tumor size. This made radical resection possible ; we were able to avoid the merger excision of great vessels and other organs. On pathological and immunopathological findings, the tumor was diagnosed as ectopic　pheochromocytoma. Regarding the safety and curability of the treatment, neoadjuvant therapy may be useful in treating very large tumors that show invasion of other organs. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 127 3 2015 血管内皮機能を対象にした基礎および臨床医学研究 187 195 EN Hirokazu Tsukahara No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 127 2 2015 薬物相互作用（33―抗菌薬の薬物相互作用） 151 154 EN Ken Tasaka Naoko Shiraishi Yoshihisa Kitamura Toshiaki Sendo No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 127 2 2015 HER2異常等の低頻度の分子異常を有する非小細胞肺癌の臨床病理学的特徴を明らかにするための前向き観察研究（HER2-CS Study）と標準化学療法後再発・増悪または標準化学療法不応性のHER2陽性非小細胞肺癌患者を対象としたトラスツズマブエムタンシン（遺伝子組換え）の第2相試験 127 132 EN Katsuyuki Kiura Katsuyuki Hotta Akiko Sato Kadoaki Ohashi Takashi Ninomiya Daisuke Minnami Masahiro Tabata Toshio Kubo Yuka Kato Taizo Hirata No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 127 2 2015 腹腔鏡内視鏡合同手術（Laparoscopy and Endoscopy Cooperative Surgery；LECS） を施行したＳ状結腸膀胱瘻の1例 123 126 EN Hiroshi Kato Masahiro Oishi Masahito Kodera Masao Yamamura Hideaki Ikeda Kenji Mizuno Yuma Tani Yutaka Yamashita Shunji Hayata Takushi Kurashige Yasuhiro Nishiyama Daisuke Nishikawa We performed laparoscopic and endoscopic cooperative surgery (LECS) for partial colectomy with partial cystectomy in an 80-year-old woman with sigmoidovesical fistula secondary to sigmoid diverticulitis. LECS was designed for local resection of the stomach for gastric submucosal tumors using the endoscopic submucosal dissection (ESD) technique. While conventional open abdominal surgery is very invasive, LECS has enabled surgeons to perform minimally invasive surgery. Although there have been no reported cases in which the bladder was laparoscopically resected after sigmoidovesical fistula division, we were able to safely resect the fistula and preserve the urethral opening by performing laparoscopic and cystoscopic cooperative surgery. There are no previously reported cases in which LECS was performed for partial sigmoidectomy and partial cystectomy ; therefore, we report this as a valuable case, with a review of the literature. We hope that further studies involving more patients will lead to the establishment of this procedure. No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 69 3 2015 Eosinophil Cationic Protein Shows Survival Effect on H9c2 Cardiac Myoblast Cells with Enhanced Phosphorylation of ERK and Akt/GSK-3β under Oxidative Stress 145 153 EN Hiroko Ishii Shigeshi Kamikawa Satoshi Hirohata Akifumi Mizutani Koji Abe Masaharu Seno Toshitaka Oohashi Yoshifumi Ninomiya Original Article 10.18926/AMO/53521 Eosinophil cationic protein (ECP) is well known as a cationic protein contained in the basic granules of activated eosinophils. Recent studies have reported that ECP exhibits novel activities on various types of cells, including rat neonatal cardiomyocytes. Here we evaluated the effects of ECP on rat cardiac myoblast H9c2 cells. Our results showed that ECP enhanced the survival of the cells, in part by promoting the ERK and Akt/GSK-3β signaling pathways. ECP attenuated the cytotoxic effects of H2O2 on H9c2 cells as well as the production of reactive oxygen species, the number of apoptotic cells and caspase 3/7 activity in the cells. In conclusion, ECP activated the ERK and Akt/GSK-3β pathways, resulting in anti-oxidative effects on H9c2 cells that attenuated apoptosis. No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 31 2015 名古屋大学動物実験支援センター東山動物実験施設 59 63 EN Mariko Itoh Hideaki Inagaki Rarami Mori Mayumi Toda Shuichi Aono Jun Sato The Institute of Laboratory Animal Research（ILAR）within the Center for Animal Research and Education (CARE), Nagoya University was established in July 2013. The aim of ILAR is to provide care for laboratory animals in safe and sanitary manner that enables researchers in the Nagoya University Higashiyama Campus to conduct proper experiments. We will now give an outline and introduce the characteristics of the new ILAR. No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 31 2015 蝸牛管側壁におけるミオイノシトール輸送体タンパク質の発現 55 58 EN Mayumi Yamaji Risa Inoue Midori Edamatsu Motonori Ando No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 日本化学繊維工業の発展と日中経済関係の展開に関する歴史的研究 −クラレにおけるレーヨン、ビニロン、人工皮革の工業化と対中技術移転を事例として− EN Masayuki Fujimoto No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 Coordination Chemistry of Ruthenium Complexes with Quinoline-2-carbaldehyde (Pyridine-2-carbonyl)hydrazone EN Asami Mori No potential conflict of interest relevant to this article was reported.

岡山大学大学院文化科学研究科 Acta Medica Okayama 1880-9162 14 2015 ベトナムから見た日本 27 45 EN Thi Thu Ha Le 10.18926/53300 No potential conflict of interest relevant to this article was reported.

岡山大学環境管理センター Acta Medica Okayama 0917-1533 36 2015 新規化学物質管理規程による化学物質管理の強化について 20 22 EN No potential conflict of interest relevant to this article was reported.

岡山大学環境管理センター Acta Medica Okayama 0917-1533 36 2015 平成26 年度環境管理センター公開講演会「環境と人と化学物質」 3 8 EN No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 127 1 2015 アンメットメディカルニーズを克服するためのプロジェクト「進行・再発乳癌に対するドセタキセル100�r/�uの第�T相試験」 41 45 EN Taizo Hirata No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2014 Behavior tests and immunohistochemical retinal response analyses in RCS rats with subretinal implantation of Okayama-University-type retinal prosthesis EN No potential conflict of interest relevant to this article was reported.

岡山大学大学院教育学研究科 Acta Medica Okayama 1883-2423 157 2014 日本とスイス-ドイツ語圏-の後期中等教育段階における化学教育 : 化学基礎教科書の比較考察 33 42 EN Kenji Tanaka Keita Tanaka 10.18926/bgeou/52948 　スイス−ドイツ語圏−のギムナジウムと日本の高等学校とにおける化学基礎に焦点を当 て，後期中等教育段階における化学教育の比較考察を行った。スイスの化学基礎は，重点化 学・補充化学とは種類が異なるとともに，それらの基礎，日本の化学基礎は，化学履修前の 基礎である。そもそも，学習指導要領などが指示しているスイスと日本の化学基礎は，履修 年限（３か１），単位数（６か２），指示する内容分野の違いから，大きく異なっていた。教 科書も，その目次からは，酸−塩基と酸化還元反応があることでは同じであるが，生化学， 環境化学の設定の有無で異なっていること，索引からは，スイスでは１／８，日本では１／ ３程度しか重複（共通）していないことなどで，大きく異なっていることを明らかにした。 No potential conflict of interest relevant to this article was reported.

岡山大学算数・数学教育学会 Acta Medica Okayama 1341-3155 21 2014 数学教育における異校種間の連携・接続の視点　―生徒の発想を引き出す学習内容の関連付けを支援する架橋教員とその働き― 103 109 EN No potential conflict of interest relevant to this article was reported.

岡山大学算数・数学教育学会 Acta Medica Okayama 1341-3155 21 2014 場合の数における児童の指向性思考からの考察 94 102 EN No potential conflict of interest relevant to this article was reported.

ヒョーロン・パブリッシャーズ Acta Medica Okayama 0289-0909 72 6 2012 文献と臨床の橋わたし　がん化学療法を受けている患者に対する口腔内管理の意義 147 149 EN No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 126 2 2014 慢性呼吸器疾患（COPD，気管支拡張症，陳旧性肺結核等）の気道感染症治療ガイドライン 151 153 EN Yasushi Tanimoto No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 126 2 2014 柿胃石の成分分析における標準物質としての柿渋の有用性 127 131 EN Masaya Iwamuro Yuko Okamoto Toshihiro Murata Yoshinari Kawai Hidenori Shiraha Hiroyuki Okada Kazuhide Yamamoto The definite diagnosis of persimmon phytobezoar (i.e., diospyrobezoar) is often accomplished by a component analysis using infrared spectroscopy. However, no studies have been conducted to investigate which substance is the best as a standard for the component analysis. Here we analyzed tannic acid, Japanese persimmon (kaki), fermented persimmon extract (kaki-shibu), conventional dried persimmon, and dried persimmon smoked in sulfur (ampo-kaki) by infrared spectroscopy to determine which would be optimal as a component analysis standard. The spectrum between 1,600 to 600cm−1 of a persimmon phytobezoar was quite similar to the spectrum of kaki-shibu rather than that of tannic acid. Consequently, we conclude that kaki-shibu should be used as a standard for infrared spectroscopy analyses of persimmon phytobezoars. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 126 2 2014 非小細胞肺癌に対する術前治療後外科切除療法 103 107 EN Shinichi Toyooka No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2014 マイクロ化学プロセス用三方電磁弁の開発と応用 EN Nobuhiro Kadowaki No potential conflict of interest relevant to this article was reported.

岡山大学環境管理センター Acta Medica Okayama 0917-1533 35 2014 化学物質管理状況監査について 31 32 EN No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 126 1 2014 術前DCF療法が著効した食道原発内分泌細胞癌の１切除例 39 43 EN Naoaki Maeda Yasuhiro Shirakawa Takeshi Koujima Toshiaki Ohara Shunsuke Tanabe Kazuhiro Noma Kazuhumi Sakurama Toshiyosi Fujiwara 　Esophageal endocrine cell carcinoma is extremely rare. We report a case of esophageal endocrine cell carcinoma showing histological complete response to neoadjuvant chemotherapy with docetaxel/cisplatin/5-fluorouracil (DCF). A 66-year-old man had been experiencing epigastralgia, and a type 2 tumor in the thoracic part of esophagus was detected by upper endoscopy. The biopsy showed endocrine cell carcinoma. PET/CT, endoscopy and an esophagogram showed that the patient had a 70-mm scaled type 2 tumor in the middle thoracic esophagus, and they also revealed lymph node metastases (no. 106recR). We diagnosed a cT3cN1cM0 cStage III tumor. With two courses of DCF treatment, both the primary tumor and lymph node metastases showed a partial response. We performed a subtotal esophagectomy with three-field lymph node dissection. The pathological examination of the resected specimens revealed no malignant cells in the esophagus or lymph nodes, and we concluded that the pathological effect of the DCF treatment was Grade 3. No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 12 2011 ある鉱物学者の誕生石図鑑�K　トルコ石 34 34 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 11 2011 ある鉱物学者の誕生石図鑑�J　トパーズ 51 51 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 10 2011 ある鉱物学者の誕生石図鑑�I　トルマリン 43 43 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 9 2011 ある鉱物学者の誕生石図鑑�H　サファイア 43 43 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 8 2011 ある鉱物学者の誕生石図鑑�G　ペリドット 47 47 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 7 2011 ある鉱物学者の誕生石図鑑�F　ルビー 51 51 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 6 2011 ある鉱物学者の誕生石図鑑�E　ムーンストーン 51 51 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 5 2011 ある鉱物学者の誕生石図鑑�D　ヒスイ 51 51 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 4 2011 ある鉱物学者の誕生石図鑑�C　ダイヤモンド 43 43 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 3 2011 ある鉱物学者の誕生石図鑑�B　アクアマリン 47 47 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 2 2011 ある鉱物学者の誕生石図鑑�A　アメジスト 35 35 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 66 1 2011 ある鉱物学者の誕生石図鑑�@　ザクロ石 46 46 EN No potential conflict of interest relevant to this article was reported.

化学同人 Acta Medica Okayama 0451-1964 67 3 2012 宝石にまつわる化学的加工技術　―偽物に騙されないための知識と心得 24 28 EN No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Study on physical and chemical clogging phenomena during artificial recharge of groundwater EN Claudia Hartwig No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Studies on Synthesis and Physicochemical Properties of Substituted [n]Phenacenes (n = 5 and 6) through Cross-Coupling Reaction/Double Cyclization Sequences EN Ning-hui Chang No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Highly Regio- and Stereoselective Synthesis of Multisubstituted Olefins via Dimetalation of Alkynylmetal Species and Chemoselective Cross-Coupling Sequences EN No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Risk factors for recurrence after transarterial chemoembolization for early-stage hepatocellular carcinoma EN Hideaki Kinugasa No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 125 3 2013 抗菌薬TDMガイドライン 251 255 EN Masatoshi Okazaki Toshiaki Sendo No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 0021-4671 30 12 1995 胃癌の術前動注化学療法に関する実験的研究 特に術後肝転移抑制効果について 1897 1907 EN No potential conflict of interest relevant to this article was reported.

岡山大学資源生物科学研究所 Acta Medica Okayama 0029-0874 59 4 1982 陸水の化学的研究 171 188 EN F. Morii J. Kobayashi S. Miyake No potential conflict of interest relevant to this article was reported.

Wiley-Blackwell Acta Medica Okayama 1347-9032 103 10 2012 Tumor growth inhibitory effect of ADAMTS1 is accompanied by the inhibition of tumor angiogenesis 1889 1897 EN Masanari Obika Hiroko Ogawa Katsuyuki Takahashi Jiayi Li Omer Faruk Hatipoglu Mehmet Zeynel Cilek Toru Miyoshi Junko Inagaki Takashi Ohtsuki Shozo Kusachi Yoshifumi Ninomiya Satoshi Hirohata Angiogenesis plays an important role in tumor progression. Several reports have demonstrated that a disintegrin and metalloproteinase with thrombospondin motifs1 (ADAMTS1) inhibited angiogenesis via multiple mechanisms. The aim of this study was to investigate the effect of ADAMTS1 on endothelial cells in vitro and on tumor growth with regard to angiogenesis in vivo. We examined the effects of the transfection of ADAMTS1 using two constructs, full-length ADAMTS1 (full ADAMTS1) and catalytic domain-deleted ADAMTS1 (delta ADAMTS1). Transfection of both the full ADAMTS1 and delta ADAMTS1 gene constructs demonstrated the secretion of tagged-ADAMTS1 protein into the conditioned medium, so we examined the effects of ADAMTS1-containing conditioned medium on endothelial cells. Both types of conditioned media inhibited endothelial tube formation, and this effect was completely abolished after immunoprecipitation of the secreted protein from the medium. Both types of conditioned media also inhibited endothelial cell migration and proliferation. We then examined the impact of ADAMTS1 on endothelial cell apoptosis. Both conditioned media increased the number of Annexin V-positive endothelial cells and caspase-3 activity and this effect was attenuated when z-vad was added. These results indicated that ADAMTS1 induced endothelial cell apoptosis. We next examined the effects of ADAMTS1 gene transfer into tumor-bearing mice. Both full ADAMTS1 and delta ADAMTS1 significantly inhibited the subcutaneous tumor growth. Collectively, our results demonstrated that ADAMTS1 gene transfer inhibited angiogenesis in vitro and in vivo, likely as a result of the induction of endothelial cell apoptosis by ADAMTS1 that occurs independent of the protease activity. No potential conflict of interest relevant to this article was reported.

岡山大学資源生物科学研究所 Acta Medica Okayama 0029-0874 62 4 1991 酵母Saccharomyces cerevisiaeのDNA修復機能欠損株を用いた化学物質の遺伝毒性評価への利用 271 281 EN Isao Aoyama No potential conflict of interest relevant to this article was reported.

岡山大学大学院教育学研究科 Acta Medica Okayama 1883-2423 153 2013 スイス−ドイツ語圏−の前期中等教育段階における物理教育―科学(理科)教科書の分析― 127 138 EN Kenji Tanaka Kenta Tanaka 10.18926/bgeou/51115 スイス−ドイツ語圏−の前期中等教育段階に焦点を当て，学習指導要領，教科書などに基 づき，物理教育の現状分析を行った。教科Naturlehre科学（理科）のもとに分野物理が位置 づけられている。学習指導要領で言及していることに対応して科学教科書も，物理，生物， 化学以外の第４の分野一般（その他）を，節単位で準備している。物理分野の節数や索引数 は，生物分野より少なく，化学分野より多かった。索引数の違いは，それぞれ，電気＞力＞ その他＞原子・核＞光＞熱＞音；人体＞動物＞植物＞その他＞一般生物；無機化学＞有機化 学＞その他＞物理化学であり，その他の割合では，生物と化学に比べて，物理は小さいこと， また，活動・発展・追加の節が準備されている割合が最も少なく，人名索引数が最も多いと いう特徴などを見いだした。 No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Structural and Biophysical Studies of Photosystem II from a Thermophilic Cyanobacterium Thermosynechococcus vulcanus EN Faisal Hammado Mekky Koua No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 星間化学・大気化学で重要なイオン・ラジカル種の振動・回転スペクトル研究 EN Ryuji Hujimori No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 125 2 2013 薬物相互作用（27―がん化学療法における制吐剤の薬物相互作用） 163 167 EN Yasuko Kurata Satoko Fujiwara Makoto Kajizono Megumu Aoyagi Yoshihisa Kitamura Toshiaki Sendo No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 125 2 2013 消化器がんと分子標的治療薬 145 152 EN Takeshi Nagasaka Toshiyoshi Fujiwara No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 125 2 2013 十二指腸濾胞性リンパ腫はAIDの発現を欠くがBACH2の発現を有しmemoryB細胞としての性質を有する 103 107 EN Katsuyoshi Takata Yasuharu Sato Naoya Nakamura Mami Tokunaka Yukari Miki Yara Yukie Kikuti Kazuhiko Igarashi Etsuro Ito Hideo Harigae Seiichi Kato Eiko Hayashi Takashi Oka Yoshinobu Hoshii Akira Tari Hiroyuki Okada ABD Alkader Lamia Mohamado Yoshinobu Maeda Mitsune Tanimoto Tomohiro Kinoshita Tadashi Yoshino No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Prognostic impact of cancer stem cell-related markers in non-small cell lung cancer patients treated with induction chemoradiotherapy EN Kazuhiko Sien No potential conflict of interest relevant to this article was reported.