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Author 山根 延元|
Published Date 1994-09-30
Publication Title
Content Type Thesis or Dissertation
Author 工藤 孝幸|
Published Date 2000-09-30
Publication Title
Content Type Thesis or Dissertation
Author 鈴木 和彦|
Published Date 1991-12-31
Publication Title
Content Type Thesis or Dissertation
Title Alternative エンドウプロトプラスト細胞における植物プロモータの発現
FullText URL 080_0051_0060.pdf
Author Yamada, Tetsuji| Hashimoto, Tadaaki| Sriprasertsak, Permpong| Kato, Hisaharu| Ichinose, Yuki| Shiraishi, Tomonori| Oku, Hachiro|
Abstract High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor.
Publication Title 岡山大学農学部学術報告
Published Date 1992
Volume volume80
Issue issue1
Start Page 51
End Page 60
ISSN 0474-0254
language English
File Version publisher
Author Masuda, Masaharu| Furusawa, Shuji|
Published Date 1991
Publication Title 岡山大学農学部学術報告
Volume volume78
Issue issue1
Content Type Departmental Bulletin Paper
Author Matsubara, Sachiko| Masuda, Seiji| Murakami, Kenji| Takahashi, Kazuhisa| Ishikura, Satoshi|
Published Date 1991
Publication Title 岡山大学農学部学術報告
Volume volume77
Issue issue1
Content Type Departmental Bulletin Paper
Author Takagi, Shigeaki| Nakano, Hiroyuki| Kimura, Yoshinobu| Kondo, Yasuhiro| Tanabe, Akira|
Published Date 1991
Publication Title 岡山大学農学部学術報告
Volume volume77
Issue issue1
Content Type Departmental Bulletin Paper
Author Masuda, Masaharu| Nagao, Hirohumi| Matsubara, Sachiko|
Published Date 1990
Publication Title 岡山大学農学部学術報告
Volume volume75
Issue issue1
Content Type Departmental Bulletin Paper
Author Matsubara, Sachiko| Chen, Dzen| Masuda, Masaharu| Murakami, Kenji|
Published Date 1990
Publication Title 岡山大学農学部学術報告
Volume volume75
Issue issue1
Content Type Departmental Bulletin Paper
Author Masuda, Masaharu|
Published Date 1989
Publication Title 岡山大学農学部学術報告
Volume volume74
Issue issue1
Content Type Departmental Bulletin Paper
Author Matsubara, Sachiko| Masuda, Masaharu| Takahashi, Kazuhisa|
Published Date 1989
Publication Title 岡山大学農学部学術報告
Volume volume74
Issue issue1
Content Type Departmental Bulletin Paper
Author Kondo, Yasuhiro| Tosa, Kaori| Tanabe, Akira| Soto, Koji|
Published Date 1987
Publication Title 岡山大学農学部学術報告
Volume volume70
Issue issue1
Content Type Departmental Bulletin Paper
Author Kondo, Yasuhiro| Ariyasu, Toshio| Tanabe, Akira| Toriumi, Tooru|
Published Date 1984
Publication Title 岡山大学農学部学術報告
Volume volume64
Issue issue1
Content Type Departmental Bulletin Paper
Author Kondo, Yasuhiro| Taniguchi, Yoshihiro| Tanabe, Akira| Toriumi, Tooru|
Published Date 1983
Publication Title 岡山大学農学部学術報告
Volume volume63
Issue issue1
Content Type Departmental Bulletin Paper
Author Kondo, Yasuhiro| Tanabe, Akira| Toriumi, Tooru|
Published Date 1983
Publication Title 岡山大学農学部学術報告
Volume volume62
Issue issue1
Content Type Departmental Bulletin Paper
Author Tanabe, Akira| Yunoki, Masashi| Noguchi, Shigeaki| Toriumi, Tooru| Kondo, Yasuhiro|
Published Date 1983
Publication Title 岡山大学農学部学術報告
Volume volume62
Issue issue1
Content Type Departmental Bulletin Paper
Title Alternative エンドウ褐紋病菌の胞子発芽液に含まれるピサチン蓄積誘導物質の分離と性質
FullText URL 059_0001_0009.pdf
Author Thanutong, Porntip| Oku, Hachiro| Shiraishi, Tomonori| Ouchi, Seiji|
Abstract An elicitor of pisatin accumulation was isolated and partially characterized from spore germination fluid, mycelial extract, and cell wall preparation of a pea pathogen, Mycosphaerella pinodes. The elicitor was found to be a polysaccharide or glycoprotein and the active component was composed of glucose. The approximate molecular weight was 70,000 daltons. This elicitor elicited pisatin accumulation in pea leaves at a concentration of 10 ug/ml and the maximum activity was ca. 350-400 ug/ml, but did not elicit phytoalexins in soybean, bean, and red clover. High molecular weight preparations from the mycelial extract and mycelial wall of Mycosphaerella melonis and Stemphylium sarcinaeforme, nonpathogens of pea, elicited pisatin accumulation in pea leaves.
Publication Title 岡山大学農学部学術報告
Published Date 1982
Volume volume59
Issue issue1
Start Page 1
End Page 9
ISSN 0474-0254
language English
File Version publisher
Author Kondo, Yasuhiro| Shiota, Masahiko| Tanabe, Akira| Toriumi, Tooru| Sato, Koji|
Published Date 1980
Publication Title 岡山大学農学部学術報告
Volume volume55
Issue issue1
Content Type Departmental Bulletin Paper
Title Alternative 殺菌剤、ベノミル、チウラムの土壌微生物相と2~3の土壌生息性糸状菌に及ぼす影響
FullText URL 054_0001_0008.pdf
Author Oku, Hachiro| Oki, Kazuo| Shiraishi, Tomonori| Sato, Kenji| Ouchi, Seiji|
Abstract Effects of Benomyl and Thiram on the soil microflora and some soil inhabitant fungi were studied using the_ soil (sandy loam) of the experimental field, Okayama University. Under field conditions, heavy application of Benomyl did not affect significantly the soil microflora. Thiram, however, reduced the fungal population in soil to 1/6 at the next day of treatment, but recovered to the normal level after 6 days. Under laboratory conditions, both fungicides did not affect soil microflora. Population of Benomyl-to lerant fungi was about 1/10 of the total fungi and increased slightly in the field soil by treatment with Benomyl at the later stage of experiment during June to October. Neither Thiram-tolerant fungus nor bacterium was found in both Thiram-treated and non-treated soils. A fungus highly tolerant to Benomyl was isolated and identified as Aspergillus versicolor, and found to not have the metabolic activity to degrade BCM. The absorption of BCM by the mycelia of this tolerant fungus, A. versicolor, was less than half of the BCM-sensitive one, such as Cladosporium harbarum.
Publication Title 岡山大学農学部学術報告
Published Date 1979
Volume volume54
Issue issue1
Start Page 1
End Page 8
ISSN 0474-0254
language English
File Version publisher
Author Tanabe, Akira| Tajiri, Shinsuke| Matsumoto, Isao| Kondo, Yasuhiro| Toriumi, Tooru|
Published Date 1978
Publication Title 岡山大学農学部学術報告
Volume volume52
Issue issue1
Content Type Departmental Bulletin Paper