result 1447 件
Author | 山根 延元| |
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Published Date | 1994-09-30 |
Publication Title | |
Content Type | Thesis or Dissertation |
Author | 工藤 孝幸| |
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Published Date | 2000-09-30 |
Publication Title | |
Content Type | Thesis or Dissertation |
Author | 鈴木 和彦| |
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Published Date | 1991-12-31 |
Publication Title | |
Content Type | Thesis or Dissertation |
Title Alternative | エンドウプロトプラスト細胞における植物プロモータの発現 |
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FullText URL | 080_0051_0060.pdf |
Author | Yamada, Tetsuji| Hashimoto, Tadaaki| Sriprasertsak, Permpong| Kato, Hisaharu| Ichinose, Yuki| Shiraishi, Tomonori| Oku, Hachiro| |
Abstract | High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor. |
Publication Title | 岡山大学農学部学術報告 |
Published Date | 1992 |
Volume | volume80 |
Issue | issue1 |
Start Page | 51 |
End Page | 60 |
ISSN | 0474-0254 |
language | English |
File Version | publisher |
Author | Masuda, Masaharu| Furusawa, Shuji| |
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Published Date | 1991 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume78 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Matsubara, Sachiko| Masuda, Seiji| Murakami, Kenji| Takahashi, Kazuhisa| Ishikura, Satoshi| |
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Published Date | 1991 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume77 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Takagi, Shigeaki| Nakano, Hiroyuki| Kimura, Yoshinobu| Kondo, Yasuhiro| Tanabe, Akira| |
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Published Date | 1991 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume77 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Masuda, Masaharu| Nagao, Hirohumi| Matsubara, Sachiko| |
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Published Date | 1990 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume75 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Matsubara, Sachiko| Chen, Dzen| Masuda, Masaharu| Murakami, Kenji| |
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Published Date | 1990 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume75 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Masuda, Masaharu| |
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Published Date | 1989 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume74 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Matsubara, Sachiko| Masuda, Masaharu| Takahashi, Kazuhisa| |
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Published Date | 1989 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume74 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Kondo, Yasuhiro| Tosa, Kaori| Tanabe, Akira| Soto, Koji| |
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Published Date | 1987 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume70 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Kondo, Yasuhiro| Ariyasu, Toshio| Tanabe, Akira| Toriumi, Tooru| |
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Published Date | 1984 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume64 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Kondo, Yasuhiro| Taniguchi, Yoshihiro| Tanabe, Akira| Toriumi, Tooru| |
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Published Date | 1983 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume63 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Kondo, Yasuhiro| Tanabe, Akira| Toriumi, Tooru| |
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Published Date | 1983 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume62 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Author | Tanabe, Akira| Yunoki, Masashi| Noguchi, Shigeaki| Toriumi, Tooru| Kondo, Yasuhiro| |
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Published Date | 1983 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume62 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Title Alternative | エンドウ褐紋病菌の胞子発芽液に含まれるピサチン蓄積誘導物質の分離と性質 |
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FullText URL | 059_0001_0009.pdf |
Author | Thanutong, Porntip| Oku, Hachiro| Shiraishi, Tomonori| Ouchi, Seiji| |
Abstract | An elicitor of pisatin accumulation was isolated and partially characterized from spore germination fluid, mycelial extract, and cell wall preparation of a pea pathogen, Mycosphaerella pinodes. The elicitor was found to be a polysaccharide or glycoprotein and the active component was composed of glucose. The approximate molecular weight was 70,000 daltons. This elicitor elicited pisatin accumulation in pea leaves at a concentration of 10 ug/ml and the maximum activity was ca. 350-400 ug/ml, but did not elicit phytoalexins in soybean, bean, and red clover. High molecular weight preparations from the mycelial extract and mycelial wall of Mycosphaerella melonis and Stemphylium sarcinaeforme, nonpathogens of pea, elicited pisatin accumulation in pea leaves. |
Publication Title | 岡山大学農学部学術報告 |
Published Date | 1982 |
Volume | volume59 |
Issue | issue1 |
Start Page | 1 |
End Page | 9 |
ISSN | 0474-0254 |
language | English |
File Version | publisher |
Author | Kondo, Yasuhiro| Shiota, Masahiko| Tanabe, Akira| Toriumi, Tooru| Sato, Koji| |
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Published Date | 1980 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume55 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |
Title Alternative | 殺菌剤、ベノミル、チウラムの土壌微生物相と2~3の土壌生息性糸状菌に及ぼす影響 |
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FullText URL | 054_0001_0008.pdf |
Author | Oku, Hachiro| Oki, Kazuo| Shiraishi, Tomonori| Sato, Kenji| Ouchi, Seiji| |
Abstract | Effects of Benomyl and Thiram on the soil microflora and some soil inhabitant fungi were studied using the_ soil (sandy loam) of the experimental field, Okayama University. Under field conditions, heavy application of Benomyl did not affect significantly the soil microflora. Thiram, however, reduced the fungal population in soil to 1/6 at the next day of treatment, but recovered to the normal level after 6 days. Under laboratory conditions, both fungicides did not affect soil microflora. Population of Benomyl-to lerant fungi was about 1/10 of the total fungi and increased slightly in the field soil by treatment with Benomyl at the later stage of experiment during June to October. Neither Thiram-tolerant fungus nor bacterium was found in both Thiram-treated and non-treated soils. A fungus highly tolerant to Benomyl was isolated and identified as Aspergillus versicolor, and found to not have the metabolic activity to degrade BCM. The absorption of BCM by the mycelia of this tolerant fungus, A. versicolor, was less than half of the BCM-sensitive one, such as Cladosporium harbarum. |
Publication Title | 岡山大学農学部学術報告 |
Published Date | 1979 |
Volume | volume54 |
Issue | issue1 |
Start Page | 1 |
End Page | 8 |
ISSN | 0474-0254 |
language | English |
File Version | publisher |
Author | Tanabe, Akira| Tajiri, Shinsuke| Matsumoto, Isao| Kondo, Yasuhiro| Toriumi, Tooru| |
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Published Date | 1978 |
Publication Title | 岡山大学農学部学術報告 |
Volume | volume52 |
Issue | issue1 |
Content Type | Departmental Bulletin Paper |