JE－6B　rotor（Beckman）による末梢血白血球分離の検討

JE－6B　rotor（Beckman）を用いてcounterflow　centrifugation　elutriationによる末梢血白血球の分離を試み，若干の検討を加えた。1．flow　rateが低い場合はリンパ球が大部分を占めたが，flow　rateの増加とともに頻度は減少した。2．　flow　rateの増加とともに，好塩基球の頻度が増加し，9－10ml／minでは頻度のみでなく細胞数の増加も認められた。各fractionにおけるヒスタミン含有量も同様の傾向が認められた。3．好中球のpeakはflow　rate　13ml／minであり以後減少を示した。4．最終段階で好酸球が増加傾向を示しChamber内で最も高値を示した。以上の結果よりcounterflow　centrifugation　elutriationによる末梢血白血球細胞分離は，in　vitroにおける実験に有用な手段である可能性が示唆された。

Separation of peripheral leukocytes was carried out in 5 healthy subjects by counterflow centrifugation elutriation with JE-6B rotor. 1. Number of lymphocytes was predominant at low flow rate (4.5ml/min) of counterflow centrifugation elutriation. 2. As flow rate was increased (9-10ml/min), the purity and number of basophils, and histamine content in the fraction were gradually increased. 3. The purity and number of neutrophils were gradually increased, and the purity was reached the peak at 13ml/min. 4. The purity and number of eosinophils were gradually increased at the late stage of the centrifugation elutriation. The results obtained here might suggest that cell separation by counterflow centrifugation elutriation is more useful for experiments, especially allergy testing, in vitro.