result 14761 件
| JaLCDOI | 10.18926/15298 |
|---|---|
| Title Alternative | ステロイド依存性重症難治性喘息に対する複合温泉療法の作用機序 |
| FullText URL | 063_001_006.pdf |
| Author | Tanizaki, Yoshiro| Kitani, Hikaru| Okazaki, Morihiro| Mifune, Takashi| Mitsunobu, Fumihiro| Takatori, Akimasa| Okuda, Hiroyuki| Ochi, Koji| Harada, Hideo| Kimura, Ikuro| |
| Abstract | Effects of complex spa therapy, which comprises swimming training in a hot spring pool, inhalation of iodine salt solution and fango therapy, were analyzed in patients with steroid-dependent intractable asthma (SDIA) by comparison between the subjects with and without efficacy of the therapy. 1. The complex spa therapy was effective in the patients with lower levels of FEV(1.0%) and % PEFR. 2. The complex spa therapy was not always effective in the patients with a low value of % V(25), which represents depressed airflow in the small airways. 3. The efficacy of the complex spa therapy was found in the patients showing a low value of % V(25) accompanied by BAL neutrophilia, but not found in those without increased proportion (more than 10% ) of neutrophils in BAL fluid. The results reveal that the complex spa therapy is effective in patients with SDIA showing low values of FEV(1.0%) and % PEFR, and in those with a low value of % V(25) and BAL neutrophilia. |
| Keywords | complex spa therapy (複合温泉療法) ventilatory function (換気機能) BAL neutrophilia (BAL好中球増多) bronchial asthma (気管支喘息) |
| Publication Title | 岡大三朝分院研究報告 |
| Published Date | 1992-06 |
| Volume | volume63 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 0913-3771 |
| language | English |
| File Version | publisher |
| NAID | 120002307537 |
| JaLCDOI | 10.18926/15297 |
|---|---|
| FullText URL | Mem_Fac_Eng_OU_33_2_97.pdf |
| Author | Jindai, Mitsuru| Osaki, Hirokazu| Kajihara, Yasuhiro| Munesawa, Yoshiomi| Tano Masakazu| Okamoto Katsuyuki| Nishino Akira| Okamoto Asao| Nishimori Naoki| |
| Abstract | We propose the recognition method of bridge soldering base metal on the circuits by comparing CAD figure and input image of image processing to locate the soldering iron tip accurately to secure the high quality Firstly, three dimensional CAD assembly drawing of circuits which are assembled perpendicularly in each other is projected on an imaginary two dimensional screen which is vertical to the optical axis of the camera The projected image is used as the standard CAD figure to inspect the location of the bridge soldering base metal. The positions among the bridge soldering base metals show the line state. So this line (connecting line) is used as the reference line to inspect the location of the bridge soldering base metal. The characteristics of the standard figure are represented by the connecting line, edge line and center points of base metal. Secondly, the position and gradient of connecting line among bridge soldering base metals in the input image is estimated. And the position of base metal and assembly accuracy of circuit units are calculated by comparing the shape and position of each base metal with its standard figure based on the connecting line Furthermore, the length between the opposite edges of the base metals are calculated to inspect the positions and the assemble accuracy of circuit units. |
| Publication Title | Memoirs of the Faculty of Engineering, Okayama University |
| Published Date | 1999-04-27 |
| Volume | volume33 |
| Issue | issue2 |
| Start Page | 97 |
| End Page | 103 |
| ISSN | 0475-0071 |
| language | English |
| File Version | publisher |
| NAID | 120002307283 |
| JaLCDOI | 10.18926/15289 |
|---|---|
| Title Alternative | 変形性膝関節炎患者膝関節液中の細胞外マトリックス成分と可溶性VCAM-1 |
| FullText URL | 007_2_099_103.pdf |
| Author | Tohge, Hiroko| Ichimura, Mitsuko| Sakiyama, Junko| Okamoto, Motoi| Nakata, Yasunari| Endo, Hiroshi| |
| Abstract | It is generally accepted that the number of patients with osteoarthritis (OA) of the knee must increase in the future because of the increase of the aged. The pathogenic mechanisms of OA haven't been elucidated yet, but it is supposed that mediate pannus may cause the factors for irreversible cartilage damage in the inflammatory process. Therefore it is very important to find and predict OA at the early stage. Now there were many indices to determine joint damage with OA, for example, cytokines including IL, TNF, TGF, matrix metalloproteinase (MMPs), tissue inhibitor of metalloproteinase (TIMPs), proteoglycan (PG), hyaluronate (HA), super oxide, adhesion molecules including, ICAM-1, VCAM-1 and PECAM. In this study, we measured that the soluble VCAM-1 (sVCAM-1), soluble collagen (sCOLL), heparansulfate proteoglycan (HSPG), uronic acis and CRP in synovial fluids (SF) with OA. A positive correlation between the level of sVCAM-1 and sCOLL (ρ = 0.652) was noted. However there was no factor showing a correlation with CRP. It was suggested that the levels of sVCAM-1 and sCOLL in the synovia fluid were active inflammation indices of knee damage, as the different aspects from CRP. The level of HSPG also showed a unique trend because HSPG on the cell surface was quickly digested endocytosis, so that it was not suitable for the inflammatory index in synovial fluid with OA. |
| Keywords | 変形性膝関節炎 (Osteoarthritis) 細胞外マトリックス (Extracellular matrix) 可溶性VCAM-1 (Soluble VCAM-1) 可溶性コラーゲン (Soluble Collagen) 可溶性ヘパラン硫酸プロテオグリカン (Soluble HSPG) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1997-01-31 |
| Volume | volume7 |
| Issue | issue2 |
| Start Page | 99 |
| End Page | 103 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002307341 |
| JaLCDOI | 10.18926/15269 |
|---|---|
| FullText URL | Mem_Fac_Eng_OU_35_47.pdf |
| Author | Doumae, Yukihiro| Konishi, Masami| Imai, Jun| Asada, Hideki| Kitamura, Akira| |
| Abstract | In this paper, an identification method of motor parameters for the diagnosis of rotor bar defects in the squirrel cage induction motor is proposed. It is difficult to distinguish the degree of deterioration by a conventional diagnostic method such as Fourier analysis. To overcome the difficulty, a motor simulator is used to identify the degree of deterioration of rotors in the squirrel cage induction motor. Using this method, the deterioration of rotor bars in the motor can be estimated quantitatively. |
| Publication Title | Memoirs of the Faculty of Engineering, Okayama University |
| Published Date | 2001-03-27 |
| Volume | volume35 |
| Issue | issue1-2 |
| Start Page | 47 |
| End Page | 51 |
| ISSN | 0475-0071 |
| language | English |
| File Version | publisher |
| NAID | 120002307181 |
| JaLCDOI | 10.18926/15268 |
|---|---|
| Title Alternative | ヒト血清リポタンパク質とシクロデキストリンのアガロースゲル内における相互作用 |
| FullText URL | 007_2_093_097.pdf |
| Author | Ichimura, Mitsuko| Tohge, Hiroko| Sakiyama, Junko| Mori, Shuji| Ito, Sekiko| Nakata, Yasunari| Endo, Hiroshi| |
| Abstract | Cyclodextrins (CDs) are cyclic oligosaccharides with low molecular weight. They have been known to bind to some serum lipoproteins and to form complexes. To elucidate whether each serum lipoprotein subclass could be separated by electrophoresis using CDs, we performed preliminary experiment, in which lipoprotein-CDs interaction was examined on electrophoresis with agarose gel. When the supporting agarose gel containing both α-CD and β-CD was prepared and was applied to isoelectric focusing for fractionating serum lipoproteins, apoB lipoproteins were found to be clearly separated into several fractions on this electrophoresis. This finding suggested that apoB lipoprotein may be detected as isolated form by arranging amounts of added CDs. |
| Keywords | 血清apoB含有リポタンパク質 (serum apoB lipoprotein) シクロデキストリン (cyclodextrin) 等電点電気泳動 (isoelectric focusing (IEF)) agarose gel |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1997-01-31 |
| Volume | volume7 |
| Issue | issue2 |
| Start Page | 93 |
| End Page | 97 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002307426 |
| JaLCDOI | 10.18926/15261 |
|---|---|
| Title Alternative | 現像温度および時間のマンモフイルム特性に与える影響 |
| FullText URL | 008_2_121_129.pdf |
| Author | Maruyama, Toshinori| Goto, Sachiko| Azuma, Yoshiharu| Nakagiri, Yoshitada| Shibuya, Koichi| Sugita, Katsuhiko| Takeuchi, Tomoyuki| Honda, Mitsugi| Yoshida, Akira| |
| Abstract | The influence for developing temperature and processing time within film processing conditions was investigated using four mammographic films, Konica New CM, Fuji UM-MA HC, Kodak Min-R M and Kodak EB/RA (for rapid system). And Fuji UR-2, a double-emulsion film, was used as a control. Those sensitometric strips exposed by a sensitometer were processed in the different combinations of developing temperatures ranging from 28 to 36℃, processing times from 45 to 210 sec. Average gradient, relative speed and base plus fog obtained from the measured film characteristic curves were evaluated for the different developing temperatures and times. Fuji UR-2 was scarcely affected and mammographic films were greatly affected in the different combinations without an increase in base plus fog except EB/RA. In New CM, UM-MA HC and Min-R M, the average gradients and the relative speeds increased as the developing temperature was higher and the developing time was longer, but the increases were limit on the combination of 36℃ and 210 sec in New CM and UM-MA HC. In EB/RA, the average gradients were almost constant and the relative speeds increased slightly like the double-emulsion film. These results suggested that it would be possible to contribute to dose reduction and advancement of contrast in New CM, UM-MA HC and Min-R M by changing these processing parameters. |
| Keywords | マンモグラフィ (Mammography) 現像処理パラメータ (Processing Parameter) 特性曲線 (Characteristic Curve) 平均階調度 (Average Gradient) 相対感度 (Relative Speed) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1998-01-31 |
| Volume | volume8 |
| Issue | issue2 |
| Start Page | 121 |
| End Page | 129 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002307943 |
| JaLCDOI | 10.18926/15257 |
|---|---|
| Title Alternative | 1997年岡山県下に発生した集団食中毒患者から分離された腸管出血性大腸菌EHEC O157 : H7のパルスフィールドゲル電気泳動法による遺伝子解析 |
| FullText URL | 008_2_091_095.pdf |
| Author | Miyamoto, Kanji| Sakamoto, Kazunari| Niiya, Masami| Chen, Jing| Abe, Eriko| Okazaki, Yuka| Shirai, Kiyoko| Goda, Noriko| |
| Abstract | Thirteen enterohemorrhagic Escherichia coli O157 : H7 (EHEC) isolates derived from the patients of an outbreak in the R-hospital in Okayama Japan and one isolated from the ingredients of Japanese noodles in June 1997 were analyzed by molecular typing using pulsed-field gel electrophoresis (PFGE). The PFGE patterns of the patients were almost the same as the patterns of the Japanese noodle ingredients. Therefore, the EHEC O157 : H7 derived from the food was considered to have caused the outbreak in the R-hospital. The molecular typing of isolates from the patients and the Japanese noodle ingredients was almost the same as that of isolates from outbreaks in Hiroshima and Fukuoka prefectures classified as type Ia in 1996 by PFGE analysis. These results indicate that EHEC O157 : H7 strains with a similar PFGE type Ia pattern have already spread throughout western Japan since last year. |
| Keywords | EHEC O157: H7 (腸管出血性大腸菌EHEC O157 : H7) PFGE (パルスフィールドゲル電気泳動法) outbreak (集団感染) molecular typing (遺伝子解析) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1998-01-31 |
| Volume | volume8 |
| Issue | issue2 |
| Start Page | 91 |
| End Page | 95 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002307383 |
| JaLCDOI | 10.18926/15254 |
|---|---|
| Title Alternative | ヒスチジンタグを持つホスファカンコア蛋白の大腸菌での発現と精製 |
| FullText URL | 009_2_105_111.pdf |
| Author | Ito, Sekiko| Okamoto, Motoi| Mori, Shuji| |
| Abstract | Specific regions of core protein of phosphacan, one of the chondroitin sulfate proteoglycans, were expressed as fusion proteins with histidine-tag (His-tag) in Escherichia coli (E.coli) and were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) matrix. cDNA fragments encoding amino acid residues 343-446 (P3) and 1-340 (P4) of phosphacan core protein were amplified by polymerase chain reaction from E18 rat brain mRNA as template. The amplified products were subcloned into pQE30 vector and were introduced into E.coli strain M15 [pREP4] for the expression. The His-tagged fusion proteins were expressed by cultivating the transformants at 37℃ for 5h in the presence of 1mM IPTG. His-tagged P3 fusion protein (His-P3) was expressed as soluble form, and was purified using Ni-NTA matrix. His-tagged P4 fusion protein (His-P4) which was sequestered into insoluble inclusion bodies was treated with 8.0M urea to solubilize, and then was purified under denaturing conditions. |
| Keywords | phosphacan (ホスファカン) core protein (コア蛋白) His-tagged proteins recombinant protein (融合蛋白) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1999-02-26 |
| Volume | volume9 |
| Issue | issue2 |
| Start Page | 105 |
| End Page | 111 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002307302 |
| JaLCDOI | 10.18926/15252 |
|---|---|
| Title Alternative | Extended-cycle processによる乳房撮影用増感紙／フィルムシステムの画質改善 |
| FullText URL | 009_2_083_090.pdf |
| Author | Goto, Sachiko| Maruyama, Toshinori| Azuma, Yoshiharu| Nakagiri, Yoshitada| Takeda, Yoshihiro| Sugita, Katsuhiko| Kimura, Fumie| Kadohisa, Shigefumi| |
| Abstract | Extended-cycle process is the term used for a processor in which the processing time has been prolonged usually to approximate 210 seconds. It has been known that the extended-cycle process of some single-emulsion films as used for mammography may enhance film contrast and increase film speed. So the speed was increased in lower speed and higher resolution system than conventional systems by means of using the extended-cycle process in this paper. We investigated how much the resolution of the system was kept. A single screen-single emulsion combination, Konica M-100/CM-H was employed as a low speed and high resolution system. This film after exposure was processed in the different combinations of developing temperatures, 30, 32 and 34℃, and processing time of 210 seconds. On the other hand, Konica M-200/CM-H was employed as a high speed system. This film was processed in the standard-cycle processing (34℃, 90 seconds). Those systems were compared on contrast, speed, screen-film blur and noise by a characteristic curve, MTF (modulation transfer function) and WS (wiener spectrum). Furthermore, the RMI 165 phantom was used to evaluate visibility of mammographic details of these systems. As a result, in the extended-cycle process at the developing temperatures of 32 or 34°C and processing time of 210 seconds for M-100/CM-H, it was possible to increase the speed as much as the higher speed system, M-200/CM-H. Then the contrast, the MTF and the visibility were also improved as compared to the higher speed system. Furthermore patient dose could be reduced at the developing temperature of 34℃ and processing time of 210 seconds in M-100/CM-H. |
| Keywords | image quality mammography single screen-single emulsion film system high-resolution screen extended-cycle process |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1999-02-26 |
| Volume | volume9 |
| Issue | issue2 |
| Start Page | 83 |
| End Page | 90 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002307881 |
| JaLCDOI | 10.18926/15236 |
|---|---|
| Title Alternative | ラテンアメリカにおける肺吸虫の種類 |
| FullText URL | 012_1_001_005.pdf |
| Author | Tongu, Yasumasa| |
| Abstract | Six species of Paragonimus are presently known in Latin America. They include the following : P. mexicanus, P. peruvianus, P. amazonicus, P. inca, P. ecuadoriensis, and P. caliensis. Among them, P. peruvianus has been recognized as a synonym of P. mexicanus by Miyazaki himself, the discoverer of P. peruvianus. Furthermore, P. ecuadoriensis is suggestive of a synonym of P. mexicanus by Miyazaki. I also regard P. amazonicus, P. inca and P. caliensis as dependent species. I entertain a strong suspicion that these are synonymous with P. mexicanus. Therefore, P. mexicanus is the only species that distinctly inhabits Latin America. In the classification, in particular Paragonimus, we have to recognize the variations in the same species. |
| Keywords | Paragonimus (肺吸虫) taxonomy (分類） Latin America (ラテンアメリカ) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2001-12-25 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 5 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002308011 |
| JaLCDOI | 10.18926/15230 |
|---|---|
| Title Alternative | マイクロコントローラを組み込んだ歯科インプラント動揺測定装置の開発 |
| FullText URL | 012_2_107_117.pdf |
| Author | Kusuma Wijaya, Sastra| Oka, Hisao| Saratani, Keiji| Matsutani, Yoshio| Tatsuta, Mitsuhiro| Kawazoe, Takayoshi| Kojima, Hideki| |
| Abstract | The aim of this study was to develop a portable device (IM checker) for measuring dental implant mobility using a microcontroller. A constant amplitude and frequency vibration was applied to a dental implant model and the acceleration signal was detected using measuring probe. Then the IM score was obtained using the criteria developed in this study. We made several implant models of different implant lengths, diameters and material of Rigolac® or Molteno®. There was a linear relationship between mechanical mobility at 400 Hz of the models and the IM scores (R(2) = 0.92). The IM checker could discriminate the mobility of dental implant models in twelve measurements with P < 0.01. There was no significant difference in the means of the IM score measured by four operators with P < 0.01. The results indicated that the IM checker had sufficient reliability and could be available in dental clinics. |
| Keywords | Tooth mobility (歯の動揺) mechanical mobility (機械モビリティ) dental implant (インプラント) manual examination (触診) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2002-03-20 |
| Volume | volume12 |
| Issue | issue2 |
| Start Page | 107 |
| End Page | 117 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002307430 |
| JaLCDOI | 10.18926/15229 |
|---|---|
| Title Alternative | 加温後のtsAF8細胞の細胞周期 |
| FullText URL | 012_2_101_106.pdf |
| Author | Shibuya, Koichi| Kawasaki, Shoji| Kuroda, Masahiro| Asaumi, Jun-ichi| Kato, Hirokazu| Hiraki, Yoshio| |
| Abstract | Thermotolerance in tsAF8 cells develops during incubation at 34℃ after heating at 45℃, while it is suppressed by the following incubation at a non-permissive temperature of 39.7℃ after the same heating. The incubation temperature after heating may affect the cell cycle and consequently thermotolerance. In the present study, a relationship between the thermotolerance and the cell cycle of tsAF8 was investigated. The cell cycle fractions and DNA synthesis were measured by flow cytometry using double staining with propidium iodide and bromodeoxyuridine. When the tsAF8 cells were heated at 45℃ for 20 min, and thereafter incubated at 34℃, bromodeoxyuridine uptake in the S phase cells (DNA synthesis) was recovered to 65.1% 6 h after the heating, and the cells showed gradual accumulation in the G(2)/M phase. When the cells were incubated at 39.7℃ after heating at 45℃ for 20 min, then showed inhibition of thermotolerance development, the DNA synthesis was recovered to 15.1% temporarily 6 h after the heating, but it became 0% after 12 h, and the cells did not remarkably accumulate in any phases of the cell cycle. This inhibition of DNA synthesis at 39.7℃ was considered to be the result of cell survival decreasing by a step-down heating. However, the relationship between the thermotolerance and the cell cycle was not found out in tsAF8 cells, because the cells did not accumulate in any phases of the cell cycle under the inhibitory condition of thermotolerance. |
| Keywords | thermotolerance (温熱耐性) hyperthermia (ハイパーサーミア) tsAF8 cell cycle (細胞周期) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2002-03-20 |
| Volume | volume12 |
| Issue | issue2 |
| Start Page | 101 |
| End Page | 106 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002307264 |
| JaLCDOI | 10.18926/15228 |
|---|---|
| Title Alternative | 放射線エネルギーの違いによる喉頭癌における吸収線量の影響 |
| FullText URL | 012_2_091_099.pdf |
| Author | Aoyama, Hideki| Nakagiri, Yoshitada| Inoue, Mitsuhiro| Inamura, Keiji| Tahara, Seiji| Uno, Hirofumi| Morioka, Yasuki| Hiraki, Yoshio| |
| Abstract | We investigated the usefulness of Gafchromic MD-55 film (Nuclear Associates, Inc.) for measuring the radiation doses on the radiotherapy of laryngeal cancers. Since larynx has thin wedge-shaped structure in anterior neck adjacent to airway, the radiation doses to the lesion may be diminished because of build-up and build-down. So, the dose has been measured with conventional measuring systems such as thermoluminescent dosimetry (TLD). However, it was not possible to evaluate the dose distribution correctly using TLD, because it is impossible to float a TLD chip in an air cavity. In this study, we employed Gafchromic MD-55 film as a dosimeter, for it can be set on the area of interest and with a measurability of dose range of 3 to 100 Gy, though it has no energy dependency. And this radiometer is composition near the soft tissue of the human body. The dose distributions to larynx were investigated with this film using neck phantom under each radiation beam energy of 4, 6 and 10 MV x-rays. Our neck phantom is made from acrylic resin and simulates a normal larynx on the basis of image information of computed tomography (CT). Moreover we observed secondary build-up and build-down curves in tissue in the vicinity of air cavities, especially at 10 MV x-rays. These findings suggest that patients with TI-T2 glottic cancers with anterior commissure invasion may receive more effective treatment with 4 MV x-rays rather than with 6 MV and 10 MV x-rays. |
| Keywords | Gafchromic MD-55-2 film radiation dose build-up build-down glottic cancer |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2002-03-20 |
| Volume | volume12 |
| Issue | issue2 |
| Start Page | 91 |
| End Page | 99 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002307342 |
| JaLCDOI | 10.18926/15220 |
|---|---|
| Title Alternative | 危機に立つ助産師教育 |
| FullText URL | 013_1_001_005.pdf |
| Author | Goda, Noriko| Nakatsukasa, Yachiyo| |
| Abstract | Midwifery education in Japan falls far short of the international standard with respect to the course requirements and the length of the clinical training. Student midwives in Japan are required to assist ten deliveries during the designated two-month clinical practicum, a difficult task to satisfy due to Japan's low birthrate. In contrast, midwifery education in Western countries takes between 12 and 48 months to finish and the E.U. standard requires students to assist at least 40 deliveries during their clinical training. Midwifery training in Japan had been traditionally offered as a non-degree certificate program at private institutions. However, four-year universities have started to offer the program as an elective program. At four-year universities, some midwifery course requirements have been deleted and the length of the clinical training has been shortened because of the congested curriculum. Serious discussion is necessary regarding the improvement of midwifery education in Japan. |
| Keywords | Midwife (助産師) Clinical training (臨床実習) Midwifery Education (助産学) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2002-12-25 |
| Volume | volume13 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 5 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002307306 |
| JaLCDOI | 10.18926/15168 |
|---|---|
| Title Alternative | Electrical Cell-substrate Impedance Sensing (ECIS)法を用いた培養細胞の微細挙動の定量的評価法 - 細胞-細胞間隙と細胞-電極間隙の評価 - |
| FullText URL | 017_1_009_015.pdf |
| Author | Goda, Noriko| Yamamoto, Yoshitake| Nakamura, Takao| Kusuhara, Toshimasa| Maruyama, Toshinori| |
| Abstract | We have proposed a mathematical model for the micro-dynamics for cultured cells measured with ECIS system for the detection of nanometer-order dynamics of cells cultured on a small gold electrode and could separately evaluate cell-to-cell distance (A) and cell-to-substrate distance (h). For wide applications of this method, we constructed mathematical models which express cell-to-electrode impedances for some kinds of confluent conditions. Based on this mathematical model, we defined new parameters S(A) and S(h) in order to evaluate cell-to-cell distance and cell-to-substrate distance. As the application, we investigated the effect of X-irradiation to bovine aortic endothelial cell (BAEC). We analyzed the micro-dynamics of cells from the impedance of BAEC before and after X-irradiation. It was proved that the stimulation of 100 Gy X-irradiation to the BAEC resulted in the large scale of increase in the cell-to-cell distances (A), and the slight increase in the cell-to-substrate distances (h) accompany with continuous fluctuations. |
| Keywords | ECIS method (ECIS法) bio-electrical impedance (生体インピーダンス) cultured cell modeling (細胞培養) micromotion of cultured cells (細胞微細挙動) X-irradiation (放射線被曝) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2007-03-15 |
| Volume | volume17 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 15 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002307368 |
| JaLCDOI | 10.18926/15166 |
|---|---|
| FullText URL | Mem_Fac_Eng_OU_31_1_19.pdf |
| Author | Totsuji, Hiroo| Kishimoto, Tokunari| Totsuji, Chieko| |
| Abstract | As a model of dusty plasmas in external fields, Yuka.wa system in a one-dimensional external field is analyzed by molecular dynamics simulations and theoretical approaches. It is shown that particles form clear thin layers (sheets) at low temperatures and the number of layers changes discretely with characteristic parameters of the system, accompanying the rearrangements of whole system from nearly equipartitioned layers to also nearly equipartitioned layers. The number, positions and populations of layers are obtained as functions of characteristic parameters. The shell (sheet) model which has been successful for confined one-component plasmas is extended to this system and results of numerical experiments are reproduced to a good accuracy. The effect of cohesive energy in each layer is of essential importance to reproduce discrete changes in the number of sheets. |
| Publication Title | Memoirs of the Faculty of Engineering, Okayama University |
| Published Date | 1996-12-27 |
| Volume | volume31 |
| Issue | issue1 |
| Start Page | 19 |
| End Page | 33 |
| ISSN | 0475-0071 |
| language | English |
| File Version | publisher |
| NAID | 120002307616 |
| JaLCDOI | 10.18926/15164 |
|---|---|
| FullText URL | Mem_Fac_Eng_OU_35_41.pdf |
| Author | Kuroboshi, Manabu| Tanaka, Muneaki| Kishimoto, Suguru| Goto, Kentaro| Tanaka, Hideo| |
| Abstract | In 360 females, Bone mineral density (BMD) in lumbar vertebrae (L2-L4) was assessed by quantitative computed tomography (QCT), and the values obtained were compared with the frequency of vertebral transformation or fracture as assessed by lateral scan image (scanogram) by X-ray CT. A correlation was observed between the frequency of vertebral transformation (or fracture) and lumbar BMD values : BMD under 125 mg/cm(3) was observed over 90% of women with vertebral transformation, and BMD under 70mg/cm(3) was found about 50% of them. These results suggest that decrease in BMD in lumbar vertebrae leads to vertebral transformation or fracture. Thus, measurement of BMD by QCT would be very useful in predicting vertebral transformation or fractures. |
| Publication Title | Memoirs of the Faculty of Engineering, Okayama University |
| Published Date | 2001-03-27 |
| Volume | volume35 |
| Issue | issue1-2 |
| Start Page | 41 |
| End Page | 46 |
| ISSN | 0475-0071 |
| language | English |
| File Version | publisher |
| NAID | 120002307948 |
| JaLCDOI | 10.18926/15155 |
|---|---|
| Title Alternative | MASTシステムによる気管支喘息患者の特異的IgE抗体の検討 |
| FullText URL | 065_062_068.pdf |
| Author | Mitsunobu, Fumihiro| Kitani, Hikaru| Mifune, Takashi| Kajimoto, Kazuhiro| Yokota, Satoshi| Takada, Ichiro| Tanizaki, Yoshiro| |
| Abstract | The serum levels of specific IgE antibodies to food allergens and inhalant allergens were evaluated in ninety five patients with bronchial asthma using the MAST system, which can measure specific IgE antibodies against sixteen different allergens with a single sample. 1. Higher positivities of specific IgE antibodies were found in inhaled allergens than in food allergens. Specific IgE antibodies against several allergens were detected in individuals more frequently in inhaled allergens than in food allergens. 2. Positivities of specific IgE antibodies against food allergens did not correlate to patients' age and patients' age at onset. In inhaled allergens, positive results in MAST system were frequently observed in patients under age of 50 and under age at onset of 60. 3. In subjects with high serum IgE levels, high positivities of specific IgE antibodies against inhaled allergens and food allergen were found. 4. The MAST system is suggested to be clinically more useful than RAST. |
| Keywords | bronchial asthma (気管支喘息) specific IgE antibodies (特異的IgE抗体) MAST system (MASTシステム) |
| Publication Title | 岡大三朝分院研究報告 |
| Published Date | 1994-09 |
| Volume | volume65 |
| Start Page | 62 |
| End Page | 68 |
| ISSN | 0918-7839 |
| language | English |
| File Version | publisher |
| NAID | 120002307337 |
| JaLCDOI | 10.18926/15154 |
|---|---|
| Title Alternative | 気管支喘息における血清免疫グロブリン値,末梢血リンパ球数と気管支肺胞洗浄液中の細胞成分との関連 |
| FullText URL | 065_055_061.pdf |
| Author | Mitsunobu, Fumihiro| Kitani, Hikaru| Mifune, Takashi| Kajimoto, Kazuhiro| Yokota, Satoshi| Takata, Ichiro| Tanizaki, Yoshiro| Ochi, Koji| Harada, Hideo| Tada, Shinya| Harada, Mine| |
| Abstract | Changes in airway inflammation by glucocorticoids were examined in 26 patients with bronchial asthma, divided into two age groups ; 20-59 and 60+ years, in relation to serum immunoglobulin levels and peripheral lymphocyte count. 1. The levels of IgG, IgA and IgM were significantly lower in patients with steroid-dependent intractable asthma (SDIA) of age over 60 years than in those with non-SDIA of the same age group. In patients between the ages of 20 and 59, only serum IgG level was significantly lower in SDIA than in non-SDIA patients. 2. Peripheral lymphocyte count was significantly decreased in SDIA patients than in non-SDIA patients in age group over 60, but not in age group between 20 and 59. 3. Number of lymphocytes in bronchoalveolar lavage (BAL) fluid was significantly lower and number of BAL neutrophils was significantly higher in SDIA patients than in non-SDIA patients in age group over 60, but not in age group between 20 and 59. These results suggest that levels of IgG, IgA and IgM, and peripheral lymphocyte count were significantly suppressed in SDIA patients of age over 60, accompanied with decrease of BAL lymphocytes and increase of BAL neutrophils. |
| Keywords | serum immunoglobulin levels (血清免疫グロブリン値) lymphocytes (リンパ球) neutrophils (好中球) bronchial asthma (気管支喘息) glucocorticoids (副腎皮質ホルモン) |
| Publication Title | 岡大三朝分院研究報告 |
| Published Date | 1994-09 |
| Volume | volume65 |
| Start Page | 55 |
| End Page | 61 |
| ISSN | 0918-7839 |
| language | English |
| File Version | publisher |
| NAID | 120002307607 |
| JaLCDOI | 10.18926/15152 |
|---|---|
| Title Alternative | ステロイド依存性重症難治性喘息における気道反応の特徴 |
| FullText URL | 065_040_047.pdf |
| Author | Mifune, Takashi| Kitani, Hikaru| Mitsunobu, Fumihiro| Kajimoto, Kazuhiro| Yokota, Satoshi| Takata, Ichiro| Tanizaki, Yoshiro| Ochi, Koji| Harada, Hideo| Tada, Shinya| Harada, Mine| |
| Abstract | Characteristics of airway response in steroid-dependent intractable asthma (SDIA) were examined in 86 asthma patients (43 with SDIA and 43 with non-SDIA) divided into three age groups: 20-39, 40-59 and 60+ years, by observing cellular composition of bronchoalveolar lavage (BAL) fluid and ventilatory function. 1. The level of serum cortisol was significantly lower in patients with SDIA than in those with non-SDIA in all age groups. 2. The proportion of lymphocytes in BAL fluid was significantly decreased in patients with SDIA compared to results in non-SDIA patients in the age between 40-59, and over 60+ years, While BAL neutrophils were significantly increased in SDIA patients compared to results in non-SDIA patients in the age group over 60 years. 3. Of ventilatory parameters, the values of % FVC, FEV(1.0%), % MMF and % V(25) were significantly lower in SDIA patients over the age of 60 compared with non-SDIA subjects of the same age group. These results show that in SDIA patients the proportion of BAL lymphocytes decreases and the proportion of BAL neutrophils increases with aging, and that ventilatory function in SDIA patients also decreases with aging compared to non-SDIA patients. |
| Keywords | bronchial asthma (気管支喘息) bronchoalveolar cells (気管支肺胞洗浄液中の細胞) ventilatory function (換気機能) glucocorticoids (グルココーチコイド) serum cortiosol levels (血清コーチゾール値) |
| Publication Title | 岡大三朝分院研究報告 |
| Published Date | 1994-09 |
| Volume | volume65 |
| Start Page | 40 |
| End Page | 47 |
| ISSN | 0918-7839 |
| language | English |
| File Version | publisher |
| NAID | 120002307402 |
