Quantitative studies of nucleic acids in the cell with microspectrophotometer. II. Nucleic acid and hemoglobin contents in erythroid cells of frog and hen

Using the erythroid cells of Rana nigromaculata the hemoglobin synthesis has been studied in the relation of DNA and RNA contents. Results showed that the hemoglobin synthesis starts in the early stage of erythroblast but becomes marked just before the complete maturation. RNA contents drops markedly in the later stage of maturation. Measurement of DNA contents by Feulgen reaction suggested the termination of the mitosis just before the prematuration. From these results the author concludes that the RNA which will act as the template for the globin synthesis, develops from the early stage of erythroblast but the templation is accelerated in the terminal stage of maturation and the marked acceleration in hemoglobin synthesis in this stage.