result 13726 件
| JaLCDOI | 10.18926/fest/11442 |
|---|---|
| FullText URL | 011_099_101.pdf |
| Author | Uddin Md. Azhar| Tanihara Manbu| Muto, Akinori| Sakata, Yusaku| |
| Abstract | Liquid phase oxidation of benzene to phenol was carried out with O(2) using carbon-based Fe-Pd and Cu-Pd catalysts at 40℃. The carbon-based Fe-Pd and Cu-Pd catalyst were prepared by modified carbothermal reduction of metal ion exchange method using cation exchange resin as a source of carbon. Fe/carbon, Cu/carbon and Pd/carbon were not effective for the oxidation of benzene to phenol with O(2) when H(2) was used as a catalyst reducing agent, however both Fe/carbon and Cu/carbon catalysts revealed activities when ascorbic acid was used as a reducing agent. Bimetallic catalysts such as Fe-Pd/carbon and Cu-Pd/carbon showed much higher activity for phenol production than the monometallic catalysts even when H(2) was used a reducing agent. Catalytic activity of Fe-Pd catalyst was much (ca. 2.5 times) higher than the Cu-Pd/catalyst. A physical mixture of the single component catalysts such as Fe and Pd or Cu and Pd were not effective in the oxidation of benzene to phenol, suggesting that an intimate contact between Fe or Cu species with Pd species is necessary to formulate an active catalyst. |
| Keywords | oxidation of benzene synthesis of phenol metal-carbon catalyst |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2006-03-15 |
| Volume | volume11 |
| Issue | issue1 |
| Start Page | 99 |
| End Page | 101 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313983 |
| JaLCDOI | 10.18926/fest/11441 |
|---|---|
| FullText URL | 012_077_081.pdf |
| Author | Khan Md. H. R.| Mohiuddin M.| Rahman M.| Akhtar M. S.| Oki, Yoko| Adachi, Tadashi| |
| Abstract | Studies on the existing non-symbiotic diazotrophic systems still are the most promising for better use of biological nitrogen fixation (BNF) in agriculture. The possibilities for the extension of nitrogen fixation to rice plants still speculative. The prospect of extension of N(2)-fixation to other plants was originally formulated to simulate the possibilities for the biological use of atmospheric nitrogen in order to overcome the ecological and economical problems of nitrogenous fertilizers. In view of this, the present study was conducted for the characterization and identification of N(2)-fixing bacterial strains at the maturity (110 days) stage in rhizosphere of rice (BR 10, Oryza sativa L.) grown in Brahmaputra Alluvium soil of Bangladesh. The soil is characterized as 'Inceptisol' order and 'Aquept' suborder. It was identified as 'Dhamrai series', had 'silt' texture, pH 6.0 and 6.8 C/N ratio. The present results of the microbial tests on the rice rhizosphere soil demonstrated that out of 401 isolates, only 94 were branded as nitrogen fixing organisms per gram of soil, which is about 23.4% of the total isolates. Based on the selection criteria, four individual strains were selected for identification. Biochemical tests were conducted for proper identification. They were identified as Closteridium spp., Klebsiella spp., Bacillus spp. and Azospirllum spp. |
| Keywords | Azospirllum spp. Bacillus spp. BNF Closteridium spp. Diazotrophs Klebsiella spp. Oryza sativa L. |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2007-03-15 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 77 |
| End Page | 81 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313950 |
| JaLCDOI | 10.18926/fest/11439 |
|---|---|
| FullText URL | 012_071_076.pdf |
| Author | Chen W.| Yoshida R.| Kawara, Osami| |
| Abstract | The Asahi River Dam reservoir is one of the important fresh water resources in Okayama, Japan, and its eutrophication has been noted since the 1980s. Therefore, aeration has been applied to control the growth of phytoplankton. In this study, we discussed the effect of aeration and water-intake depth on the water quality distribution in the reservoir, based on numerical simulation and observation. The principal conclusions were as follows:(1) The numerical simulation models applied here reproduced observations fairly well. (2) Water intake from the surfacce layer reduced the concentration of chlorophyll a in the surface layers. Water intake from the middle or bottom layer improved the deficit of dissolved oxygen in the bottom layers. (3) Aeration together with water intake from the middle or bottom layer improved the deficit of dissolved oxygen in the bottom layers. |
| Keywords | the Asahi River Dam Reservoir numerical simulation eutrophication water intake depth dissolved oxygen chlorophylla |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2007-03-15 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 71 |
| End Page | 76 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002314056 |
| JaLCDOI | 10.18926/fest/11437 |
|---|---|
| FullText URL | 011_075_081.pdf |
| Author | Dincsoy Enver Erdinc| Ichiminami, Fumikazu| |
| Abstract | This study aims to examine the Southeastern Anatolia Project in Turkey, which contains irrigation, energy and drinking water development schemes. The project is the biggest regional development effort ever undertaken by Turkish Government and has influenced the sustainable economic and human development targets. With the completion of each step of the project, it has been expected that there have been many important economic and social changes in Turkish regions, especially the southeast part of Turkey (called as "Southeastern Anatolia Region") and its surrounding areas. The project also interests in both Turkey and its related regions and sustainability is a major issue of concern. Following a brief introduction of the project, the paper examines the type of recent social-economic changes in the region and Turkey in terms of sustainable development components. Under the light of our investigations from different perspectives, it is observed that GAP region with its development project is very far from expectations in the point of sustainability. |
| Keywords | Sustainability Southeastern Anatolia Region GAP Regional Development GDP per capita Turkey |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2006-03-15 |
| Volume | volume11 |
| Issue | issue1 |
| Start Page | 75 |
| End Page | 81 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313496 |
| JaLCDOI | 10.18926/fest/11436 |
|---|---|
| FullText URL | 012_063_070.pdf |
| Author | Saif Eideen S. A.| Taniguchi, Takeo| |
| Abstract | The homogenization method is used to model steel fiber reinforced concrete SFRC by converting the random distribution of fibers to a periodic one. The periodic distribution is chosen to hold similar properties of the composite material in both perpendicular directions to represent an average approximation for the random distribution. The material is modeled as a composite with brittle matrix and elastic fibers. Two patterns of the unit cell are examined to establish the homogenized stiffiness matrix in elastic and plastic stages. A rigid plastic bonding is considered between matrix and fibers. The smeared crack model is used to represent the nonlinearity of concrete. The validity of the homogenized model is examined by comparing the numerical results with the experimental results. The results show good agreement with the experimental work when a suitable pattern of the unit cell is used. |
| Keywords | fiber reinforced concrete composite materials homogenization periodicity finite element |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2007-03-15 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 63 |
| End Page | 70 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313536 |
| JaLCDOI | 10.18926/fest/11435 |
|---|---|
| FullText URL | 011_065_074.pdf |
| Author | Dincsoy Enver Erdinc| Ichiminami, Fumikazu| |
| Abstract | Regional development and regional planning subjects are very closely linked with the development projects in Turkey as much as in the world. The main applications and studies are usually based on the elimination of disparities among regions. Within this framework, this study has firstly aimed to set out the regional development objectives in Turkey. Secondly, we tried to find out the effect of new regional approaches on Turkish regional structure. Hence, as an approach different from other studies, we focused on NUTS (Nomenclature of Territorial Units for Statistics) regions instead of geographical units (regions) studies. In the following parts of the study, thirdly, the efforts of Turkey about the regional development have been discussed and assessed by using Neoclassic Growth Model in terms of per capita income distributions for new region groups. To compare the results of analysis among regional units, the most important development projects for Turkey were also taken as a different approach unlike other sudies. Previous studies based on geographic regions showed that the basic tendency of per capita income distribution is 'Divergence' unlike expected 'Convergence' in Turkey, but in our study, we found more optimistic results for Turkey, in which per capita income distributions have a tendency to converge after 1997. |
| Keywords | Regional Development Turkey NUTS Regional Development Projects Convergence |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2006-03-15 |
| Volume | volume11 |
| Issue | issue1 |
| Start Page | 65 |
| End Page | 74 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002314049 |
| JaLCDOI | 10.18926/fest/11434 |
|---|---|
| FullText URL | 012_051_061.pdf |
| Author | Liu Xianbing| Tanaka, Masaru| Matsui, Yasuhiro| |
| Abstract | Serious adverse impacts on the environment and human health from the recycling and disposal of electronic waste have occurred in the past and continue to occur in China today, due to the lack of a national management strategy. With aiming to support the management strategy development, a study was carried out to plan and quantitatively evaluate the optional management frameworks for the selected five main types of large electronic home appliances in Beijing, the capital city and a typically big municipality in China. This paper outlined the main findings yielded from the series of assessment studies which started from the generation amount prediction and material flow analysis of the used electronic appliances, planning and optimization of the collection & transportation frameworks and ended at the economic evaluation of the optional recycling processes for the waste appliances. Although the revenue could be expected from the result of isolated evaluation of the recycling processes, the entire system will be economically unavailable if the used appliances are still collected from the owners at current prices. The traditional understanding of householders on the values of their used appliances should be changed in Beijing and China as a whole. Establishment of a formal collection system that could take back the used appliances at lower prices (less than 40% of the current level) is the key for the construction of a formal management framework with sustainability. |
| Keywords | Waste electronic home appliances Management framework Assessment Beijing China |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2007-03-15 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 51 |
| End Page | 61 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313493 |
| JaLCDOI | 10.18926/fest/11432 |
|---|---|
| FullText URL | 012_041_049.pdf |
| Author | Chaerul Mochammad| Tanaka, Masaru| V. Shekdar Ashok| |
| Abstract | In Indonesia, municipal solid waste (MSW) is becoming increasing complex due to variety of reasons like the increasing quantity of MSW, rising public awareness and municipal administration policies in different cities and surrounding regencies. After the landslide accident at Bandung city disposal site, most of related agencies are trying their level best to improve the situation. Against this backdrop, this paper attempts to analyze the present system of MSW addressing variety of aspects such as quantity and composition of MSW being generated, operational management, legal system as well as financial aspect. The systematic assessment has revealed the problem like lack of legal framework, low coverage, improper waste storage, less encouragement for composting, and lack of proper disposal practices. Finally, an action plan is presenting suggestion for immediate and future addressing the issues like the operational management, institutional, financial aspect, public participation & environmental education. |
| Keywords | municipal solid waste strategic actions Indonesia |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2007-03-15 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 41 |
| End Page | 49 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313633 |
| JaLCDOI | 10.18926/fest/11431 |
|---|---|
| FullText URL | 011_051_055.pdf |
| Author | Nishigaki, Makoto| Komatsu, Mitsuru| Akudago John Apambilla| Shinshi Yoshihide| Kawakami Kenji| Kumamaru Koji| |
| Abstract | A laboratory validation of a proposed new method of determining the in-situ effective porosity of unsaturated soils was carried out on unsaturated river sand. The proposed method consists of boring a small diameter hole into the soil and inserting an Amplitude Domain Reflectometry (ADR) probe at the bottom part of the hole. Water is supplied into the hole till saturation and later de-saturated. The water content with time is determined from the ADR probe voltage potential readings. The effective porosity is determined from the difference between the saturated and de-saturated water contents. However, in the laboratory, the water is supplied through the top and bottom parts of the sample. From the experiments the obtained effective porosity ranged from 0.28 to 0.29 for wetting from the top with an average of 0.287 and 0.29 to 0.30 for wetting from the bottom with an average of 0.293. The determined effective porosity ranged from 71.7-73.3% of the real initial porosity of 0.40 of the river sand. The maximum attained degree of saturation during the experiment was 91%. The results indicated that the method will be suitable and useful in determining the effective porosity of medium grained unsaturated soils. |
| Keywords | Underground dam Effective porosity Storage capacity Freshwater development |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2006-03-15 |
| Volume | volume11 |
| Issue | issue1 |
| Start Page | 51 |
| End Page | 55 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313335 |
| JaLCDOI | 10.18926/fest/11430 |
|---|---|
| FullText URL | 011_043_049.pdf |
| Author | Saif Eldeen S. A.| Taniguchi, Takeo| |
| Abstract | The simulation of mechanical bahavior of steel fiber reinforced concrete SFRC is introduced utilizing the homogenization method. The random distribution of fibers has been converted to a periodic distribution. Using the periodicity assumption, the boundary conditions for the unit cell are derived. The homogenized stiffness matrix is determined in elastic and plastic range. A numerical example to study the effect of the volume fraction of fibers is introduced. |
| Keywords | fiber reinforced concrete composite materials homogenization periodicity finite element |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2006-03-15 |
| Volume | volume11 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 49 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313548 |
| JaLCDOI | 10.18926/fest/11424 |
|---|---|
| FullText URL | 011_009_014.pdf |
| Author | Nishina Tomohiko| Tian Tian Chen| Fujita Kazutoshi| Ishikawa, Hirofumi| |
| Abstract | The red fox (Vulpes vulepes) and the vole ( Clethrionomys refocanus) are principal hosts of Echinococcus multilocularis in Hokkaido, Japan. How protoscoleces increases in voles and the level of immunity in foxes remain unknown because of the lack of survey data, so that it is important to clarify these mecahnisms in order to develop control strategies against E. multilocularis. In this study, the growth of protoscoleces in the infected voles was approximated as the logistic curve, the level of immunity in the fox was assumed to depend on the experience of the infection with E. multilocularis, and the worm burden in the fox was assumed to be governed by the amount of protoscoleces in the vole. Our model showed that the population densities of the hosts and the level of immunity influenced the prevalence of the E. multilocularis. |
| Keywords | Echinococcus multilocularis protoscoleces immunity worm burden stochastic model |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2006-03-15 |
| Volume | volume11 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 14 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002314034 |
| JaLCDOI | 10.18926/fest/11423 |
|---|---|
| FullText URL | 012_007_018.pdf |
| Author | Imura Keigo| XiaoDong Wang| Ishikawa, Hirofumi| |
| Abstract | The elliptic curve cryptosystem is a popular cryptosystem. Its safety depends on the difficulty of the elliptic curve discrete logarithm problem (ECDLP). From the viewpoint of ECDLP, it is very interesting to determine the order of elliptic curves. We tabulate the order of elliptic curves on the finite field of characteristic two using the Schoof algorithm, which is an efficient algorithm to decide orders. The Schoof algorithm is carried out by O(log(8)q). Because the calculation of y(q2) occupies most of the time used to execute the Schoof algorithm, it is necessary to reduce the amount of y(q2) calculations. |
| Keywords | elliptic curve order division polynomial Schoof algorithm finite field of characteristic two |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2007-03-15 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 7 |
| End Page | 18 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313838 |
| JaLCDOI | 10.18926/fest/11422 |
|---|---|
| FullText URL | 011_001_007.pdf |
| Author | Fujita Kazutoshi| Tian Tian Chen| Nishina Tomohiko| Ishikawa, Hirofumi| |
| Abstract | Plasmodium vivax re-emerged in 1993 near the demilitarized zone (DMZ) in South Korea, although P. vivax malaria disappeared in South Korea in 1979. The re-emergence of malaria in South Korea is believed to have originated from infection by mosquitoes from North Korea across the DMZ. The principal vector of P. vivax in the Korean Peninsula is Anopheles sinensis. The density of An. sinensis has a peak during the second week of July. The North Korean strain of P. vivax has 2 characteristics: a wide distribution of the terms of relapse and a high rate of relapse. Therefore, we may well wonder why the incidence of malaria is concentrated in summer, especially in August. Mathematical models in North Korea and South Korea were constructed in which the South Korean model was affected unidirectionally by the North Korean model. We carried out simulations of the model for the Paju-shi and Yonchon-gun situations near the DMZ region. The simulation results followed the time-course of the re-emergence of P. vivax there, and revealed the mechanism of the elevation of the incidence of P. vivax in summer. |
| Keywords | DMZ Korea model Plasmodium vivax re-emergence |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2006-03-15 |
| Volume | volume11 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 7 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 40007297549 |
| JaLCDOI | 10.18926/fest/11421 |
|---|---|
| FullText URL | 012_001_006.pdf |
| Author | Watanabe, Masaji| Kawai, Fusako| |
| Abstract | A mathematical model for exogenous depolymerization with time dependent degradation rate is analyzed in order to study the biodegradation of polyethylene glycol. The weight distribution with respect to the molecular weight before and after cultivation of microorganisms were analyzed an inverse problem to determine the time dependent degradation rate. Numerical techniques to solve the inverse problem and to simulate the transition of the weight distribution are described. |
| Keywords | biodegradation polyethylene glycol mathematical model numerical simulation |
| Publication Title | 岡山大学環境理工学部研究報告 |
| Published Date | 2007-03-15 |
| Volume | volume12 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 1341-9099 |
| language | English |
| File Version | publisher |
| NAID | 120002313599 |
| JaLCDOI | 10.18926/11369 |
|---|---|
| Title Alternative | 語い教授における対話スクリプトを使ったオーセンティックな話言葉の規範の使用 |
| FullText URL | 001_099_112.pdf |
| Author | C. J. Creighton| |
| Abstract | This investigate teaching pragmatic competence, specifically the appropriate register. The subjects were 34 2nd year EFL students enrolled in "Basic Conversation", over one semester, at a Japanese national university. Students wrote and performed 4 scripted dialogues as part of an ABAB single case study where the intervention was focusing exercises of optimal features of the oral register authentic NS dialogues. Comparison of the baseline and subsequent scripts using the Wilcoxon Signed Ranks Test (non-parametric) showed a significant increase in the use of spoken features. Student feedback and instructor observation also provided corroboration. The results point to the utility of authentic materials in the Japanese EFL classroom. |
| Keywords | pragmatic competence (語用論的能力) oral register (口語的な語い) authentic language (オーセンティックな話し言葉) scripted dialogues (対話スクリプト) ABAB single case study |
| Publication Title | 岡山大学教育実践総合センター紀要 |
| Published Date | 2001 |
| Volume | volume1 |
| Issue | issue1 |
| Start Page | 99 |
| End Page | 112 |
| ISSN | 1346-3705 |
| language | English |
| File Version | publisher |
| NAID | 120002313476 |
| Title Alternative | ラン科植物に発生するシンビジウムモザイクウイルスの血清学的検出 |
|---|---|
| FullText URL | 005__001_039_046.pdf |
| Author | I Wayan, Gara| Kondo, Hideki| Maeda, Takanori| |
| Abstract | Dot-immunobinding assay (DIA) on nitrocellulose membranes and rapid immunofilter paper assay (RIPA) were examind for their usefulness in the detection of cymbidium mosaic virus (CyMV) in orchids. The minimum detection levels of CyMV by these methods were 100 ng/ml in purified preparations and at 10-4 dilution of extracts from infected leaves of orchids could be detected by these methods. Although DIA took 5 to 6 hours for the detection of the virus, it was reliable method for diagnosis of a large-number of samples. On the other hand, RIPA, which enabled detection of CyMV within a few minutes with sensitivity similar to that of DIA, will be suitable as a rapid and handy tool for virus disease diagnosis in orchids. Moreover, by RIPA, we could detect CyMV and odontoglossum ringspot virus (ORSV) simultaneously form doubly infected plant. |
| Keywords | Serological detection Cymbidium mosaic virus Orchid |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 39 |
| End Page | 46 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313874 |
| Title Alternative | 日本におけるCymbidium属植物から分離されて生物学的性質の異なるOdontoglossum Ringspot Virus分離株のペプチドマッピングによる比較 |
|---|---|
| FullText URL | 005_001_031_038.pdf |
| Author | Kondo, Hideki| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | Symptoms on Cymbidium, double-stranded (ds) RNA pattern and peptide mapping of coat protein (CP) of five isolates of odontoglossum ringspot virus from Cymbidium in Japan were compared. One of the isolates, Cy-1, that produced unique symptoms on Cymbidium, showed a distinct peptide mapping pattern from those of the other four isolates by partial digestion of CP with pepsin. All the isolates produced three major dsRNA species of Mr=4.3,1.4 and 0.6×106 in the infected plants. |
| Keywords | Odontoglossum ringspot virus Cymbidium Peptide mapping Double-stranded RNA |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 31 |
| End Page | 38 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313419 |
| Title Alternative | 2種のモノクローナル抗体を用いた簡易ELISAによるキュウリモザイクウイルス迅速・高感度検出 |
|---|---|
| FullText URL | 005_001_023_030.pdf |
| Author | Maeda, Takanori| Sako, Nobumichi| Inouye, Narinobu| |
| Abstract | To detect cucumber mosaic virus (CMV), virus samples and conjugate were incubated together in a simplified double-antibody sandwich ELISA. The use of the same monoclonal antibody (MAb) as trapping (coating) and detecting antibodies resulted in considerable decrease of ELISA values and sensitivity due to the competition for antigen between trapping and detecting antibodies. The simplified ELISA using two MAbs which recognize different epitopes of CMV proved to be a rapid and sensitive method for virus detection. |
| Keywords | Cucumber mosaic virus Monoclonal antibody Simplified ELISA |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 23 |
| End Page | 30 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | ジゴキシゲニン標識プローブを用いたBeet Necrotic Yellow Vein Virus RNA の検出 |
|---|---|
| FullText URL | 005_001_079_096.pdf |
| Author | Saito, Minako| Kiguchi, Tadahiko| Tamada, Tetsuo| |
| Abstract | Complementary DNA (cDNA) clones corresponding to each of five distinct RNA species of beet necrotic yellow vein (BNYVV) were synthesized and identified. The sizes of each cDNA clone for RNAs 1,2,3,4 and 5 molecules were 3.0, 1.7, 1.8, 1.5 and 1.4 kbp, respectively. cDNA inserts to RNA 2 were covered at a part of the 3'regions, and those of RNAs 3,4 and 5 were almost full-length. The plasmids containing each of cDNA inserts were labeled with digoxigenin by the random priming method. Northern blot hybridization tests showed that individual probes hybridized specially to each of the five RNAs. Good results were obtained with 1 to 10 ng of RNA as a mixture of five RANs, but the probe to RNA 3, RNA 4 or RNA 5 gave a weak signal with hererologous RNAs when more than 10 ng RNA was used. In dot blot hybridization, the limit of detection was about 10 pg RNA, but if a higher content of RNA was spotted, cross reaction occurred using heterologous RNAs. For laboratory and field isolates of BNYVV, each of RNAs 3,4 and 5 was easily detected by Northern blot hybridization in total nucleic acids extracted from Tetragonia expansa leaves inoculated mechanically, but not from roots of sugar-beet plants inoculated by the fungus Polymyxa betae. However, satisfactory results were obtained with partially purified or concentrated preparations from roots. These findings indicate that the digoxigenin-labeled probes are useful for the identification and detection of RNAs contained in field and laboratory isolates of BNYVV. |
| Keywords | Sugar beet Rhizomania BNYVV RNA detection Nonradioactive cDNA probe |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 79 |
| End Page | 96 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | ライムギ小型染色体を保持する普通系コムギからのライムギ型cDNAのディファレンシャルスクリーニング |
|---|---|
| FullText URL | 006_001_053_064.pdf |
| Author | Murata, Minoru| |
| Abstract | Occurrence of the midget chromosome in a common wheat with rye cytoplasm [(cereale)-Chinese Spring (CS)] indicates that the chromosome carries the essential gene(s) for maintaining the function of rye cytoplasm. To elucidate the interaction between the midget chromosome and rye cytoplasm, in this study, an attempt was made to isolate rye-type cDNAs from a cDNA library of (cereale)-CS by differential screening. Two replica filters from each plate were hybridized with digoxigenin (DIG)-labeled wheat CS cDNAs and with DIG-labeled rye cDNAs,respectively. Out of ca. 20,000 plaques, 27 were hybridized more strongly with rye cDNAs than with CS cDNAs. These clones were classified into six classes (Ⅰ-Ⅵ) by blot hybridization. The majority of the clones (21 out of 27) was belonged to the same class (1), showing rye-type RFLP (restriction fragment length polymorphism). The DNA sequence of clone CrClA in class Ⅰ, was very similar to that of wheat ribulose 1,5-bisphosphate carboxylase,large subnit gene, rbcL(94.5% homology). However, the 3' end of CrClA was shorter than that of wheat rbcL, and terminated at TAA instead of TAG, like the rbcL of Aegilops crassa. In the clone CrC5.4, the first half of the sequence was similar to that of one rice EST clone, the functions of which are not known, and the latter was similar to the reverse sequence of maize 4.5S-23S ribosomal RNA. This suggests that CrC5.4 had been derived from two defferent cDNAs of (cereale)-CS. Three other clones had homology to the chlorophyll a/b binding protein genes (cab) of wheat, maize and tomato, and one to wheat rbcS (ribulose1,5-bisphosphate carboxylase small subnit gene). However, no clear polymorphisms were detected between wheat and rye by using those clones as probes. |
| Keywords | Cytoplasm substitution line Differential screening Midget chromosome Rye Wheat |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1999 |
| Volume | volume6 |
| Issue | issue1 |
| Start Page | 53 |
| End Page | 64 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
