ヒト組織内リンパ球Subpopulationの分布に関する研究 第l編 組織内リンパ球subpopulationの分布同定法の基礎的検討および各リンパ組織におけるその分布

Thymus-derived lymphocytes (T-cells) and bone marrow-derived lymphocytes (B-cells) in the cryosection of human tissues were identified either by rosette formation with sheep erythrocytes (E) or with erythrocytes sensitized with antibody and complement (EAC), respectively. Invariable and stable reaction was obtained in EAC rosettes of the tissue lymphocytes while E binding of them was rather unstable. E could be adhered in the thymus sections which were obtained from normal infants during open heart surgery, but EAC did not bind to them at all. It was confirmed that B-cells located mostly in the lymph follicles of the lymph nodes as well as the palatine tonsils and T-cells were present predominantly in the paracortical area and interfollicular region. B-cells in the germinal centers would generally have more activated C(3) receptors than those of the mantle zones. In addition, B-cells of the lymph follicles both in the lymph nodes and in the palatine tonsils could be also detected utilizing the membrane immunofluorescence technique. The proportion of T and B-cells distribution in the lymphoid tissue was well correlated with that obtained on free cell suspension technique.Therefore, it could be concluded that T and B lymphocytes were able to identify respectively even in tissue level.