Studies on the cornin extracted from bovine liver. II. Inhibitory effect of the cornin on DNA synthesis and cell growth of L cells cultured in suspension

Gornin was extracted from bovine liver. The effects of cornin on DNA synthesis were compared with its effects on cell growth using L cells growing in suspension. As the first step of this experiment, a simple method of suspension culture was established with a new modification of YLE medium. Both effects of cornin paralleled with dosage. And the properties of the inhibitory factor of DNA synthesis are the same as those of growth inhibitor in respect to the heat stability and impermeability against dialyzing membrane. The inhibitor of DNA synthesis could not be separated from that of growth by gel filtration with Sephadex G-75.