Establishment of a transgenic strain for the whole brain calcium imaging in larval medaka fish (Oryzias latipes)

Seki, Takahide; Miyanari, Kazuhiro; Shiraishi, Asuka; Tsuda, Sachiko; Ansai, Satoshi; Takeuchi, Hideaki

doi:10.1016/j.neures.2025.104944

Permalink : https://ousar.lib.okayama-u.ac.jp/69189

ID	69189
FullText URL	fulltext.pdf 8.69 MB suppl.pdf 1.37 MB
Author	Seki, Takahide Graduate School of Life Sciences, Tohoku University Miyanari, Kazuhiro Graduate School of Science and Engineering, Saitama University Shiraishi, Asuka Graduate School of Science and Engineering, Saitama University Tsuda, Sachiko Graduate School of Science and Engineering, Saitama University Ansai, Satoshi Ushimado Marine Institute, Okayama University Takeuchi, Hideaki Graduate School of Life Sciences, Tohoku University
Abstract	GCaMP-based calcium imaging is a powerful tool for investigating neural function in specific neurons. We generated transgenic (Tg) medaka strains expressing jGCaMP7s across extensive brain regions under the control of the gap43 promoter. Using these Tg larvae, calcium imaging successfully detected a tricaine-induced suppression of spontaneous neural activity and topographical visual responses in the optic tectum elicited by moving paramecia or optical fiber stimulation. These results indicate that our Tg medaka strains provide a versatile platform for investigating neural dynamics and their responses to various stimuli.
Keywords	gap43 JGCaMP7s Ac/Ds Visuotopy slc2a15b
Published Date	2025-10
Publication Title	Neuroscience Research
Volume	volume219
Publisher	Elsevier BV
Start Page	104944
ISSN	0168-0102
NCID	AA10641652
Content Type	Journal Article
language	English
OAI-PMH Set	岡山大学
Copyright Holders	© 2025 Published by Elsevier B.V.
File Version	publisher
PubMed ID	40769387
DOI	10.1016/j.neures.2025.104944
Related Url	isVersionOf https://doi.org/10.1016/j.neures.2025.104944
License	http://creativecommons.org/licenses/by-nc-nd/4.0/
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