start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Neutrophil-to-lymphocyte ratio affects the impact of proton pump inhibitors on efficacy of immune checkpoint inhibitors in patients with non?small-cell lung cancer
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background The neutrophil-to-lymphocyte ratio (NLR) at the initiation of immune checkpoint inhibitor (ICI) therapy is a known predictor of prognosis. Proton pump inhibitors (PPIs) reportedly attenuate the therapeutic efficacy of ICIs. However, the attenuation effects are not consistently observed across all patients. This study aimed to evaluate whether NLR serves as a stratification factor to determine the impact of PPI on the efficacy of ICI.<br>
Methods This retrospective study was conducted in patients with NSCLC treated with ICI monotherapy. Patients were stratified into two groups (higher NLR (??4) and lower NLR (<?4)). PPI use was defined as the administration of PPIs within 30 days before or after ICI initiation. The primary outcome was progression-free survival (PFS) and the secondary outcome was overall survival (OS).<br>
Results Among the 132 patients included, PPI users exhibited significantly shorter median PFS and OS than non-PPI users. In the higher NLR group (n?=?61), PPI users had a markedly shorter PFS and OS than non-PPI users (median PFS: 1.6 vs. 8.2 months; p?<?0.01, median OS: 3.3 vs. 19.6 months; p?=?0.015). Conversely, in the lower NLR group (n?=?71), no significant difference in PFS and OS was observed between PPI users and non-PPI users (median PFS: 2.8 vs. 7.3 months, p?=?0.83, median OS: 17.6 vs. 24.4 months, p?=?0.40).<br>
Conclusion NLR may be a significant stratification factor for evaluating the impact of PPI on PFS and OS in patients with NSCLC undergoing ICI monotherapy.
en-copyright=
kn-copyright=

en-aut-name=HoriTomoki
en-aut-sei=Hori
en-aut-mei=Tomoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamamotoKazuhiro
en-aut-sei=Yamamoto
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ItoTakefumi
en-aut-sei=Ito
en-aut-mei=Takefumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IkushimaShigeki
en-aut-sei=Ikushima
en-aut-mei=Shigeki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OmuraTomohiro
en-aut-sei=Omura
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YanoIkuko
en-aut-sei=Yano
en-aut-mei=Ikuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Pharmacy, Nara Prefecture General Medical Center
kn-affil=

affil-num=2
en-affil=Department of Integrated Clinical and Basic Pharmaceutical Science, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Respiratory Medicine, Nara Prefecture General Medical Center
kn-affil=

affil-num=4
en-affil=Department of Pharmacy, Nara Prefecture General Medical Center
kn-affil=

affil-num=5
en-affil=Department of Pharmacy, Kobe University Hospital
kn-affil=

affil-num=6
en-affil=Department of Pharmacy, Kobe University Hospital
kn-affil=
en-keyword=Immune checkpoint inhibitor
kn-keyword=Immune checkpoint inhibitor
en-keyword=Neutrophil-to-lymphocyte ratio
kn-keyword=Neutrophil-to-lymphocyte ratio
en-keyword=Non-small-cell lung cancer
kn-keyword=Non-small-cell lung cancer
en-keyword=Proton pump inhibitor
kn-keyword=Proton pump inhibitor
END

start-ver=1.4
cd-journal=joma
no-vol=4
cd-vols=
no-issue=3
article-no=
start-page=e70167
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250728
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Occupational therapist‐guided exercise increased white blood cell and neutrophil counts during clozapine treatment: A case report
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Moderate exercise increases white blood cells and neutrophils. However, there are no reports on the relationship between exercise intensity and these cells. We observed a patient taking clozapine whose white blood cell and neutrophil counts were borderline. Supervised exercise therapy with an occupational therapist stabilized these counts.<Br>
Case Presentation: A 50-year-old woman with treatment-resistant schizophrenia was prescribed clozapine. By Day 63, the clozapine dosage had been increased to 450?mg/day. Additionally, she was advised to perform a 30-min walking exercise program 1 h before blood tests. Exercise therapy supervised by an occupational therapist was performed eight times, and self-training was performed five times. Exercise intensity was monitored using the Borg Scale for subjective evaluation and the Karvonen formula for objective evaluation. Supervised exercise therapy with an occupational therapist resulted in greater increases on the Borg Scale and Karvonen formula than did self-training. It also induced increases in white blood cells and neutrophils. Her psychiatric symptoms improved, and she was discharged on Day 71. A blood test taken after discharge revealed that her white blood cell and neutrophil counts were within the normal range and she continued to take clozapine for 2 years. She has since been able to enjoy a calm and relaxed life at home.<br>
Conclusion: Exercise involving subjective and objective evaluation by an occupational therapist effectively increased white blood cells and neutrophils during clozapine treatment. Supervised exercise therapy by an occupational therapist is important when self-exercise is insufficient for continuing clozapine treatment.
en-copyright=
kn-copyright=

en-aut-name=HinotsuKenji
en-aut-sei=Hinotsu
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SakamotoShinji
en-aut-sei=Sakamoto
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KawaiHiroki
en-aut-sei=Kawai
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OhyaYoshio
en-aut-sei=Ohya
en-aut-mei=Yoshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YokodeAkiyoshi
en-aut-sei=Yokode
en-aut-mei=Akiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AsadaTakahiro
en-aut-sei=Asada
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OkahisaYuko
en-aut-sei=Okahisa
en-aut-mei=Yuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TakakiManabu
en-aut-sei=Takaki
en-aut-mei=Manabu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Neuropsychiatry, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Neuropsychiatry, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Neuropsychiatry, Okayama University Hospital
kn-affil=

affil-num=5
en-affil=Department of Neuropsychiatry, Okayama University Hospital
kn-affil=

affil-num=6
en-affil=Department of Neuropsychiatry, Okayama University Hospital
kn-affil=

affil-num=7
en-affil=Department of Neuropsychiatry, Okayama University Hospital
kn-affil=

affil-num=8
en-affil=Department of Neuropsychiatry, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=clozapine
kn-keyword=clozapine
en-keyword=exercise
kn-keyword=exercise
en-keyword=leukopenia
kn-keyword=leukopenia
en-keyword=neutropenia
kn-keyword=neutropenia
en-keyword=occupational therapist
kn-keyword=occupational therapist
END

start-ver=1.4
cd-journal=joma
no-vol=60
cd-vols=
no-issue=10
article-no=
start-page=1151
end-page=1159
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=202412
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=NCF-1 plays a pivotal role in the survival of adenocarcinoma cells of pancreatic and gastric origins
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Reactive oxygen species (ROS) play a pivotal biological role in cells, with ROS function differing depending on cellular conditions and the extracellular environment. Notably, ROS act as cytotoxic factors to eliminate infectious pathogens or promote cell death under cellular stress, while also facilitating cell growth (via ROS-sensing pathways) by modifying gene expression. Among ROS-related genes, neutrophil cytosolic factor-1 (NCF-1; p47phox) was identified as a ROS generator in neutrophils. This product is a subunit of a cytosolic NADPH oxidase complex activated in response to pathogens such as bacteria and viruses. NCF-1 has been examined primarily in terms of ROS-production pathways in macrophages and neutrophils; however, the expression of this protein and its biological role in cancer cells remain unclear. Here, we report expression of NCF-1 in pancreatic and gastric cancers, and demonstrate its biological significance in these tumor cells. Abundant expression of NCF-1 was observed in pancreatic adenocarcinoma (PDAC) lines and in patient tissues, as well as in gastric adenocarcinomas. Accumulation of the protein was also detected in the invasive/metastatic foci of these tumors. Unexpectedly, BxPC-3 underwent apoptotic cell death when transfected with a small interfering RNA (siRNA) specific to NCF-1, whereas the cells treated with a control siRNA proliferated in a time-dependent manner. A similar phenomenon was observed in HSC-58, a poorly differentiated gastric adenocarcinoma line. Consequently, the tumor cells highly expressing NCF-1 obtained coincident accumulation of ROS and reduced glutathione (GSH) with expression of glutathione peroxidase 4 (GPX4), a quencher involved in ferroptosis. Unlike the conventional role of ROS as a representative cytotoxic factor, these findings suggest that NCF-1-mediated ROS generation may be required for expansive growth of PDAC and gastric cancers.
en-copyright=
kn-copyright=

en-aut-name=Furuya-IkudeChiemi
en-aut-sei=Furuya-Ikude
en-aut-mei=Chiemi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KittaAkane
en-aut-sei=Kitta
en-aut-mei=Akane
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TomonobuNaoko
en-aut-sei=Tomonobu
en-aut-mei=Naoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KawasakiYoshihiro
en-aut-sei=Kawasaki
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SakaguchiMasakiyo
en-aut-sei=Sakaguchi
en-aut-mei=Masakiyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KondoEisaku
en-aut-sei=Kondo
en-aut-mei=Eisaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Division of Tumor Pathology, NIR-PIT Research Institute, Kansai Medical University
kn-affil=

affil-num=2
en-affil=Division of Tumor Pathology, NIR-PIT Research Institute, Kansai Medical University
kn-affil=

affil-num=3
en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Division of Tumor Pathology, NIR-PIT Research Institute, Kansai Medical University
kn-affil=

affil-num=5
en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Division of Tumor Pathology, NIR-PIT Research Institute, Kansai Medical University
kn-affil=
en-keyword=NCF-1 (p47phox)
kn-keyword=NCF-1 (p47phox)
en-keyword=ROS
kn-keyword=ROS
en-keyword=Cancer
kn-keyword=Cancer
en-keyword=Tumor growth
kn-keyword=Tumor growth
en-keyword=Apoptosis
kn-keyword=Apoptosis
END

start-ver=1.4
cd-journal=joma
no-vol=15
cd-vols=
no-issue=1
article-no=
start-page=27163
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250725
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Eosinophils as a predictive marker of treatment-related adverse events in mRCC patients treated with first-line immune-checkpoint inhibitor combination therapy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Immune checkpoint inhibitors (ICIs) are a key component of first-line treatment for metastatic renal cell carcinoma (mRCC). However, predicting treatment-related adverse events (TRAEs) remains challenging. This study investigated the utility of eosinophil-related biomarkers as predictors of Common Terminology Criteria for Adverse Events grade???3 TRAEs in mRCC patients undergoing ICI combination therapy. In this retrospective analysis across 21 hospitals in Japan, we examined 180 patients treated with ICI/ICI therapy and 216 patients treated with ICI/tyrosine kinase inhibitor (TKI) therapy. Grade???3 TRAEs occurred in 39.4% and 31.9% of patients in the ICI/ICI and ICI/TKI groups, respectively. An elevated eosinophil proportion of???2.0% (odds ratio [OR]: 2.36; 95% CI [confidence interval] 1.23?4.54, p?=?0.01) and a low neutrophil/eosinophil ratio (NER) of???40.0 (OR: 2.78, 95% CI 1.39?5.53, p?=?0.004) were significant predictors of severe TRAEs in the ICI/ICI group. However, no significant associations were found in the ICI/TKI group. These findings may help identify patients who suffer from grade???3 TRAEs and help determine individualized treatment strategies in patients with mRCC.
en-copyright=
kn-copyright=

en-aut-name=KawadaTatsushi
en-aut-sei=Kawada
en-aut-mei=Tatsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KatayamaSatoshi
en-aut-sei=Katayama
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YanagisawaTakafumi
en-aut-sei=Yanagisawa
en-aut-mei=Takafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MoriKeiichiro
en-aut-sei=Mori
en-aut-mei=Keiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=FukuokayaWataru
en-aut-sei=Fukuokaya
en-aut-mei=Wataru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KomuraKazumasa
en-aut-sei=Komura
en-aut-mei=Kazumasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TsujinoTakuya
en-aut-sei=Tsujino
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MaenosonoRyoichi
en-aut-sei=Maenosono
en-aut-mei=Ryoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TakaharaKiyoshi
en-aut-sei=Takahara
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=NukayaTakuhisa
en-aut-sei=Nukaya
en-aut-mei=Takuhisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=InokiLan
en-aut-sei=Inoki
en-aut-mei=Lan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=ToyodaShingo
en-aut-sei=Toyoda
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=HashimotoTakeshi
en-aut-sei=Hashimoto
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=HirasawaYosuke
en-aut-sei=Hirasawa
en-aut-mei=Yosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=EdamuraKohei
en-aut-sei=Edamura
en-aut-mei=Kohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=KobayashiTomoko
en-aut-sei=Kobayashi
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=BekkuKensuke
en-aut-sei=Bekku
en-aut-mei=Kensuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=NishimuraShingo
en-aut-sei=Nishimura
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=IwataTakehiro
en-aut-sei=Iwata
en-aut-mei=Takehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=SadahiraTakuya
en-aut-sei=Sadahira
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=TominagaYusuke
en-aut-sei=Tominaga
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=YamanoiTomoaki
en-aut-sei=Yamanoi
en-aut-mei=Tomoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

en-aut-name=YoshinagaKasumi
en-aut-sei=Yoshinaga
en-aut-mei=Kasumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=

en-aut-name=TsuboiKazuma
en-aut-sei=Tsuboi
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=24
ORCID=

en-aut-name=KobayashiYasuyuki
en-aut-sei=Kobayashi
en-aut-mei=Yasuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=25
ORCID=

en-aut-name=TakamotoAtsushi
en-aut-sei=Takamoto
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=26
ORCID=

en-aut-name=KuroseKyohei
en-aut-sei=Kurose
en-aut-mei=Kyohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=27
ORCID=

en-aut-name=KimuraTakahiro
en-aut-sei=Kimura
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=28
ORCID=

en-aut-name=AzumaHaruhito
en-aut-sei=Azuma
en-aut-mei=Haruhito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=29
ORCID=

en-aut-name=ShirokiRyoichi
en-aut-sei=Shiroki
en-aut-mei=Ryoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=30
ORCID=

en-aut-name=FujitaKazutoshi
en-aut-sei=Fujita
en-aut-mei=Kazutoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=31
ORCID=

en-aut-name=OhnoYoshio
en-aut-sei=Ohno
en-aut-mei=Yoshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=32
ORCID=

en-aut-name=ArakiMotoo
en-aut-sei=Araki
en-aut-mei=Motoo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=33
ORCID=

affil-num=1
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Urology, The Jikei University School of Medicine
kn-affil=

affil-num=4
en-affil=Department of Urology, The Jikei University School of Medicine
kn-affil=

affil-num=5
en-affil=Department of Urology, The Jikei University School of Medicine
kn-affil=

affil-num=6
en-affil=Department of Urology, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=7
en-affil=Department of Urology, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=8
en-affil=Department of Urology, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=9
en-affil=Department of Urology, Fujita Health University School of Medicine
kn-affil=

affil-num=10
en-affil=Department of Urology, Fujita Health University School of Medicine
kn-affil=

affil-num=11
en-affil=Department of Urology, Kindai University Faculty of Medicine
kn-affil=

affil-num=12
en-affil=Department of Urology, Kindai University Faculty of Medicine
kn-affil=

affil-num=13
en-affil=Department of Urology, Tokyo Medical University
kn-affil=

affil-num=14
en-affil=Department of Urology, Tokyo Medical University
kn-affil=

affil-num=15
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=17
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=18
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=19
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=20
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=21
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=22
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=23
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=24
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=25
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=26
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=27
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=28
en-affil=Department of Urology, The Jikei University School of Medicine
kn-affil=

affil-num=29
en-affil=Department of Urology, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=30
en-affil=Department of Urology, Fujita Health University School of Medicine
kn-affil=

affil-num=31
en-affil=Department of Urology, Kindai University Faculty of Medicine
kn-affil=

affil-num=32
en-affil=Department of Urology, Tokyo Medical University
kn-affil=

affil-num=33
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Renal cell carcinoma
kn-keyword=Renal cell carcinoma
en-keyword=Immune checkpoint inhibitor
kn-keyword=Immune checkpoint inhibitor
en-keyword=ICI
kn-keyword=ICI
en-keyword=Eosinophil
kn-keyword=Eosinophil
en-keyword=Immune-related adverse event
kn-keyword=Immune-related adverse event
en-keyword=Treatment-related adverse event
kn-keyword=Treatment-related adverse event
END

start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=6
article-no=
start-page=388.e1
end-page=388.e14
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202506
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Clinical effects of granulocyte colony-stimulating factor administration and the timing of its initiation on allogeneic hematopoietic cell transplantation outcomes for myelodysplastic syndrome
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Granulocyte colony-stimulating factor (G-CSF) accelerates neutrophil recovery after allogeneic hematopoietic cell transplantation (HCT). However, the optimal use of G-CSF and the timing of its initiation after allogeneic HCT for myelodysplastic syndrome (MDS) according to graft type have not been determined. This retrospective study aimed to investigate the effects of using G-CSF administration and the timing of its initiation on transplant outcomes in adult patients with MDS undergoing allogeneic HCT. Using Japanese registry data, we retrospectively investigated the effects of G-CSF administration and the timing of its initiation on transplant outcomes among 4140 adults with MDS after bone marrow transplantation (BMT), peripheral blood stem cell transplantation (PBSCT), or single-unit cord blood transplantation (CBT) between 2013 and 2022. Multivariate analysis showed that early (days 0 to 4) and late (days 5 to 10) G-CSF administration significantly accelerated neutrophil recovery compared with no G-CSF administration following BMT, PBSCT, and CBT, but there was no benefit of early G-CSF initiation for early neutrophilic recovery regardless of graft type. Late G-CSF initiation was significantly associated with a higher risk of overall chronic GVHD following PBSCT (hazard ratio [HR], 1.63; 95% confidence interval [CI], 1.18 to 2.24; P = .002) and CBT (HR, 2.09; 95% CI, 1.21 to 3.60; P = .007) compared with no G-CSF administration. Late G-CSF initiation significantly improved OS compared with no G-CSF administration only following PBSCT (HR, 0.74; 95% CI, 0.58 to 0.94; P = .015). However, G-CSF administration and the timing of its initiation did not affect acute GVHD, relapse, or non-relapse mortality, irrespective of graft type. These results suggest that G-CSF administration significantly accelerated neutrophil recovery after BMT, PBSCT, and CBT, but increased risk of overall chronic GVHD after PBSCT and CBT. However, the effect of early and late G-CSF initiation on transplant outcomes needs further study in adult patients with MDS.

en-copyright=
kn-copyright=

en-aut-name=KonumaTakaaki
en-aut-sei=Konuma
en-aut-mei=Takaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=FujiokaMachiko
en-aut-sei=Fujioka
en-aut-mei=Machiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FuseKyoko
en-aut-sei=Fuse
en-aut-mei=Kyoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HosoiHiroki
en-aut-sei=Hosoi
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MasamotoYosuke
en-aut-sei=Masamoto
en-aut-mei=Yosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=DokiNoriko
en-aut-sei=Doki
en-aut-mei=Noriko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=UchidaNaoyuki
en-aut-sei=Uchida
en-aut-mei=Naoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TanakaMasatsugu
en-aut-sei=Tanaka
en-aut-mei=Masatsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SawaMasashi
en-aut-sei=Sawa
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=NishidaTetsuya
en-aut-sei=Nishida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=IshikawaJun
en-aut-sei=Ishikawa
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=AsadaNoboru
en-aut-sei=Asada
en-aut-mei=Noboru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=NakamaeHirohisa
en-aut-sei=Nakamae
en-aut-mei=Hirohisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=HasegawaYuta
en-aut-sei=Hasegawa
en-aut-mei=Yuta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=OnizukaMakoto
en-aut-sei=Onizuka
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=MaedaTakeshi
en-aut-sei=Maeda
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=FukudaTakahiro
en-aut-sei=Fukuda
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=KawamuraKoji
en-aut-sei=Kawamura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=KandaYoshinobu
en-aut-sei=Kanda
en-aut-mei=Yoshinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=OhbikiMarie
en-aut-sei=Ohbiki
en-aut-mei=Marie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=AtsutaYoshiko
en-aut-sei=Atsuta
en-aut-mei=Yoshiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=ItonagaHidehiro
en-aut-sei=Itonaga
en-aut-mei=Hidehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

affil-num=1
en-affil=Department of Hematology/Oncology, The Institute of Medical Science, The University of Tokyo
kn-affil=

affil-num=2
en-affil=Department of Hematology, Sasebo City General Hospital
kn-affil=

affil-num=3
en-affil=Faculty of Medicine, Department of Hematology, Endocrinology and Metabolism, Niigata University
kn-affil=

affil-num=4
en-affil=Department of Hematology/Oncology, Wakayama Medical University
kn-affil=

affil-num=5
en-affil=Department of Cell Therapy and Transplantation Medicine, The University of Tokyo Hospital
kn-affil=

affil-num=6
en-affil=Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital
kn-affil=

affil-num=7
en-affil=Department of Hematology, Toranomon Hospital
kn-affil=

affil-num=8
en-affil=Department of Hematology, Kanagawa Cancer Center
kn-affil=

affil-num=9
en-affil=Department of Hematology and Oncology, Anjo Kosei Hospital
kn-affil=

affil-num=10
en-affil=Department of Hematology, Japanese Red Cross Aichi Medical Center Nagoya Daiichi Hospital
kn-affil=

affil-num=11
en-affil=Department of Hematology, Osaka International Cancer Institute
kn-affil=

affil-num=12
en-affil=Department of Hematology and Oncology, Okayama University Hospital
kn-affil=

affil-num=13
en-affil=Department of Hematology, Osaka Metropolitan University Graduate School of Medicine
kn-affil=

affil-num=14
en-affil=Department of Hematology, Hokkaido University Hospital
kn-affil=

affil-num=15
en-affil=Department of Hematology and Oncology, Tokai University School of Medicine
kn-affil=

affil-num=16
en-affil=Department of Hematology and oncology, Kurashiki Central Hospital
kn-affil=

affil-num=17
en-affil=Department of Hematopoietic Stem Cell Transplantation, National Cancer Center Hospital
kn-affil=

affil-num=18
en-affil=Department of Hematology, Tottori University Hospital
kn-affil=

affil-num=19
en-affil=Division of Hematology, Jichi Medical University
kn-affil=

affil-num=20
en-affil=Japanese Data Center for Hematopoietic Cell Transplantation
kn-affil=

affil-num=21
en-affil=Japanese Data Center for Hematopoietic Cell Transplantation
kn-affil=

affil-num=22
en-affil=Transfusion and Cell Therapy Unit, Nagasaki University Hospital
kn-affil=
en-keyword=Granulocyte colony-stimulating factor
kn-keyword=Granulocyte colony-stimulating factor
en-keyword=Graft-versus-host disease
kn-keyword=Graft-versus-host disease
en-keyword=Bone marrow transplantation
kn-keyword=Bone marrow transplantation
en-keyword=Peripheral blood stem cell transplantation
kn-keyword=Peripheral blood stem cell transplantation
en-keyword=Cord blood transplantation
kn-keyword=Cord blood transplantation
en-keyword=Myelodysplastic syndrome
kn-keyword=Myelodysplastic syndrome
END

start-ver=1.4
cd-journal=joma
no-vol=79
cd-vols=
no-issue=3
article-no=
start-page=147
end-page=155
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202506
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Immunometabolic Regulation of Innate Immunity in Systemic Lupus Erythematosus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Pathogens or their components can induce long-lasting changes in the behavior of innate immune cells, a process analogous to “training” for future threats or environmental adaptation. However, such training can sometimes have unintended consequences, such as the development of autoimmunity. Systemic lupus erythematosus (SLE) is a chronic and heterogeneous autoimmune disease characterized by the production of autoantibodies and progressive organ damage. Innate immunity plays a central role in its pathogenesis, contributing through impaired clearance of apoptotic cells, excessive type I interferon production, and dysregulated formation of neutrophil extracellular traps. Recent studies have revealed that metabolites and nucleic acids derived from mitochondria, a crucial energy production site, directly regulate type I interferon and anti-inflammatory cytokine production. These insights have fueled interest in targeting metabolic pathways as a novel therapeutic approach for SLE, offering promise for improving long-term patient outcomes.
en-copyright=
kn-copyright=

en-aut-name=WatanabeHaruki
en-aut-sei=Watanabe
en-aut-mei=Haruki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsumotoYoshinori
en-aut-sei=Matsumoto
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=systemic lupus erythematosus
kn-keyword=systemic lupus erythematosus
en-keyword=interferon
kn-keyword=interferon
en-keyword=tricarboxylic acid cycle
kn-keyword=tricarboxylic acid cycle
en-keyword=innate immune memory
kn-keyword=innate immune memory
en-keyword=trained immunity
kn-keyword=trained immunity
END

start-ver=1.4
cd-journal=joma
no-vol=17
cd-vols=
no-issue=4
article-no=
start-page=e82348
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250416
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Bilateral Scleritis and Neutrophilic Dermatosis With Cytogenetic Chromosomal Aberrancy Related to Pyoderma Gangrenosum: A Case Report of a 20-Year Follow-Up
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Pyoderma gangrenosum is a non-infectious autoimmune disease with skin plaques and ulcers in the entity of neutrophilic dermatosis and may have a background of myelodysplastic syndromes. This study reported a 20-year follow-up of a patient with pyoderma gangrenosum and scleritis who showed chromosomal aberrancy from the initial phase and later in the course developed thrombocythemia. A 51-year-old man presented with widespread indurated erythematous plaques with scaling and pustules on the forehead, bilateral eyelids, and nasal bridge, in addition to nodular scleritis in the left eye and ulcer formation of the plaques in the lower legs. Skin biopsy revealed massive dermal infiltration mainly with neutrophils in the absence of neutrophilic vasculitis. Suspected of myelodysplastic syndromes, bone marrow biopsy was normal, while chromosomal aberrancy, 46, XY, del (20) (q11q13.3), was detected. In the diagnosis of neutrophilic dermatosis, probably of pyoderma gangrenosum, he began to have oral prednisolone 20 mg daily and colchicine 1 mg daily, leading to the subsidence of skin lesions. Four months later, he developed nodular scleritis in the right eye and began to use topical 0.1% betamethasone in both eyes. He was stable with only prednisolone 12.5 mg daily until the age of 55.5 years, when he showed an increase of serum lactate dehydrogenase. The bone marrow aspirate disclosed neither blast cell increase nor atypical cells. The same chromosomal aberrancy was repeatedly detected. One year later, he developed breathing difficulty and underwent tracheostomy. Laryngeal lesion biopsy disclosed squamous cell papilloma with human papillomavirus-6. At 60 years old, he showed marginal corneal infiltration in the left eye, and at 61 years old, hypopyon in the right eye. Platelets tended to increase up to 1000 × 103/?L, and bone marrow examinations were recommended but refused by the patient. At the latest follow-up at 71 years old, he was ambulatory in health and stable with a tracheostomy cannula. In conclusion, pyoderma gangrenosum with scleritis occurred in an undetermined hematological malignancy with chromosomal aberrancy.
en-copyright=
kn-copyright=

en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TanakaTakehiro
en-aut-sei=Tanaka
en-aut-mei=Takehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AsadaNoboru
en-aut-sei=Asada
en-aut-mei=Noboru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ObikaMikako
en-aut-sei=Obika
en-aut-mei=Mikako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OmichiRyotaro
en-aut-sei=Omichi
en-aut-mei=Ryotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=IwatsukiKeiji
en-aut-sei=Iwatsuki
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Ophthalmology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Pathology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Hematology and Oncology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of General Internal Medicine, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Dermatology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=corneal infiltration
kn-keyword=corneal infiltration
en-keyword=hypopyon
kn-keyword=hypopyon
en-keyword=myelodysplastic syndromes
kn-keyword=myelodysplastic syndromes
en-keyword=neutrophilic dermatosis
kn-keyword=neutrophilic dermatosis
en-keyword=peripheral keratitis
kn-keyword=peripheral keratitis
en-keyword=pyoderma gangrenosum
kn-keyword=pyoderma gangrenosum
en-keyword=scleritis
kn-keyword=scleritis
en-keyword=sweet syndrome
kn-keyword=sweet syndrome
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=3
article-no=
start-page=96
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250204
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cancer-associated fibroblasts promote pro-tumor functions of neutrophils in pancreatic cancer via IL-8: potential suppression by pirfenidone
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background The mechanisms by which neutrophils acquire pro-tumor properties remain poorly understood. In pancreatic cancer, cancer-associated fibroblasts (CAFs) may interact with neutrophils, directing them to promote tumor progression.<br>
Methods To validate the association between CAFs and neutrophils, the localization of neutrophils was examined in clinically resected pancreatic cancer specimens. CAFs were produced by culturing in cancer-conditioned media, and the effects of these CAFs on neutrophils were examined. In vitro migration and invasion assays assess the effect of CAF-activated neutrophils on cancer cells. The factors secreted by the activated neutrophils were also explored. Finally, pirfenidone (PFD) was tested to determine whether it could suppress the pro-tumor functions of activated neutrophils.<br>
Results In pancreatic cancer specimens, neutrophils tended to co-localize with IL-6-positive CAFs. Neutrophils co-cultured with CAFs increased migratory capacity and prolonged life span. CAF-affected neutrophils enhance the migratory and invasive activities of pancreatic cancer cells. IL-8 is the most upregulated cytokine secreted by the neutrophils. PFD suppresses IL-8 secretion from CAF-stimulated neutrophils and mitigates the malignant traits of pancreatic cancer cells.<br>
Conclusion CAFs activate neutrophils and enhance the malignant phenotype of pancreatic cancer. The interactions between cancer cells, CAFs, and neutrophils can be disrupted by PFD, highlighting a potential therapeutic approach.
en-copyright=
kn-copyright=

en-aut-name=YagiTomohiko
en-aut-sei=Yagi
en-aut-mei=Tomohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KagawaShunsuke
en-aut-sei=Kagawa
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NogiShohei
en-aut-sei=Nogi
en-aut-mei=Shohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TaniguchiAtsuki
en-aut-sei=Taniguchi
en-aut-mei=Atsuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshimotoMasashi
en-aut-sei=Yoshimoto
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SuemoriKanto
en-aut-sei=Suemori
en-aut-mei=Kanto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NagaiYasuo
en-aut-sei=Nagai
en-aut-mei=Yasuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=FujitaShuto
en-aut-sei=Fujita
en-aut-mei=Shuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KurodaShinji
en-aut-sei=Kuroda
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KikuchiSatoru
en-aut-sei=Kikuchi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KakiuchiYoshihiko
en-aut-sei=Kakiuchi
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=TeraishiFuminori
en-aut-sei=Teraishi
en-aut-mei=Fuminori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=TakagiKosei
en-aut-sei=Takagi
en-aut-mei=Kosei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=OharaToshiaki
en-aut-sei=Ohara
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=TazawaHiroshi
en-aut-sei=Tazawa
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

affil-num=1
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Departments of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Cancer-associated fibroblasts
kn-keyword=Cancer-associated fibroblasts
en-keyword=Neutrophil
kn-keyword=Neutrophil
en-keyword=Anti-fibrotic agent
kn-keyword=Anti-fibrotic agent
en-keyword=Pirfenidone
kn-keyword=Pirfenidone
END

start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250102
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Predictive marker for response to trifluridine/tipiracil plus bevacizumab in metastatic colorectal cancer patients
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Objective Trifluridine/tipiracil (FTD/TPI) is one of the options for late-line treatment of colorectal cancer (CRC). However, the specific patient populations that would particularly benefit from it remain unclear. This study attempted to identify predictive markers of chemotherapy efficacy with trifluridine/tipiracil (FTD/TPI), focusing on the RNA-editing enzyme adenosine deaminase acting on RNA 1 (ADAR1) expression and neutrophil-lymphocyte ratio (NLR).<br>
Methods To assess the effectiveness of FTD/TPI in CRC patients, we retrospectively analyzed 72 CRC patients at Okayama University Hospital from 2014 to 2022.<br>
Results Adding bevacizumab to FTD/TPI resulted in a more prolonged progression-free survival (PFS), consistent with the SUNLIGHT study findings (p = 0.0028). Among the participants, those with a high NLR had a shorter PFS (p = 0.0395). Moreover, high ADAR1 expression was associated with longer PFS (p = 0.0151). In multivariate analysis, low ADAR1 (HR = 3.43, p = 0.01) and absence of bevacizumab (HR = 4.25, p = 0.01) were identified as factors shortening PFS. The high ADAR1 group demonstrated fewer cases of progressive disease and a higher proportion of stable disease than the low ADAR1 group (p = 0.0288). Low NLR and high ADAR1 were predictive markers of prolonged PFS in the bevacizumab-treated group (p = 0.0036).<br>
ConclusionLow NLR and high ADAR1 were predictive markers for a positive response to the FTD/TPI plus bevacizumab regimen associated with prolonged PFS. The FTD/TPI plus bevacizumab regimen should be proactively implemented in the low NLR and high ADAR1 subgroups.
en-copyright=
kn-copyright=

en-aut-name=TakahashiToshiaki
en-aut-sei=Takahashi
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShigeyasuKunitoshi
en-aut-sei=Shigeyasu
en-aut-mei=Kunitoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KondoYoshitaka
en-aut-sei=Kondo
en-aut-mei=Yoshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakedaSho
en-aut-sei=Takeda
en-aut-mei=Sho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=UmedaHibiki
en-aut-sei=Umeda
en-aut-mei=Hibiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MoriwakeKazuya
en-aut-sei=Moriwake
en-aut-mei=Kazuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KayanoMasashi
en-aut-sei=Kayano
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=SakuraiYuya
en-aut-sei=Sakurai
en-aut-mei=Yuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NakamuraShunsuke
en-aut-sei=Nakamura
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=TakahashiMasafumi
en-aut-sei=Takahashi
en-aut-mei=Masafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NittaKaori
en-aut-sei=Nitta
en-aut-mei=Kaori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=YoshidaKazuhiro
en-aut-sei=Yoshida
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=MatsumiYuki
en-aut-sei=Matsumi
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=MichiueHiroyuki
en-aut-sei=Michiue
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=YamamotoHideki
en-aut-sei=Yamamoto
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=KishimotoHiroyuki
en-aut-sei=Kishimoto
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=TeraishiFuminori
en-aut-sei=Teraishi
en-aut-mei=Fuminori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=ShojiRyohei
en-aut-sei=Shoji
en-aut-mei=Ryohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=KanayaNobuhiko
en-aut-sei=Kanaya
en-aut-mei=Nobuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=KashimaHajime
en-aut-sei=Kashima
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=KakiuchiYoshihiko
en-aut-sei=Kakiuchi
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=KurodaShinji
en-aut-sei=Kuroda
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

en-aut-name=KagawaShunsuke
en-aut-sei=Kagawa
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=24
ORCID=

affil-num=1
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=

affil-num=15
en-affil=Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=17
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=18
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=19
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=20
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=21
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=22
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=23
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=24
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=ADAR1
kn-keyword=ADAR1
en-keyword=Colorectal cancer
kn-keyword=Colorectal cancer
en-keyword=Biomarker
kn-keyword=Biomarker
en-keyword=Trifluridine/tipiracil
kn-keyword=Trifluridine/tipiracil
END

start-ver=1.4
cd-journal=joma
no-vol=44
cd-vols=
no-issue=2
article-no=
start-page=249
end-page=260
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20241005
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Loss of Nr4a1 ameliorates endothelial cell injury and vascular leakage in lung transplantation from circulatory-death donor
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Ischemia-reperfusion injury (IRI) stands as a major trigger for primary graft dysfunction (PGD) in lung transplantation (LTx). Especially in LTx from donation after cardiac death (DCD), effective control of IRI following warm ischemia (WIRI) is crucial to prevent PGD. This study aimed to identify the key factors affecting WIRI in LTx from DCD.<br>
Methods: Previously reported RNA-sequencing dataset of lung WIRI was reanalyzed to identify nuclear receptor subfamily 4 group A member 1 (NR4A1) as the immediate early gene for WIRI. Dynamics of NR4A1 expression were verified using a mouse hilar clamp model. To investigate the role of NR4A1 in WIRI, a mouse model of LTx from DCD was established using Nr4a1 knockout (Nr4a1?/?) mice.<br>
Results: NR4A1 was located around vascular cells, and its protein levels in the lungs increased rapidly and transiently during WIRI. LTｘ from Nr4a1?/? donors significantly improved pulmonary graft function compared to wild-type donors. Histological analysis showed decreased microvascular endothelial cell death, neutrophil infiltration, and albumin leakage. Evans blue permeability assay demonstrated maintained pulmonary microvascular barrier integrity in grafts from Nr4a1?/? donors, correlating with diminished pulmonary edema. However, NR4A1 did not significantly affect the inflammatory response during WIRI, and IRI was not suppressed when a wild-type donor lung was transplanted into the Nr4a1?/? recipient.<br>
Conclusions: Donor NR4A1 plays a specialized role in the positive regulation of endothelial cell injury and microvascular hyperpermeability. These findings demonstrate the potential of targeting NR4A1 interventions to alleviate PGD and improve outcomes in LTx from DCD.
en-copyright=
kn-copyright=

en-aut-name=KawanaShinichi
en-aut-sei=Kawana
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkazakiMikio
en-aut-sei=Okazaki
en-aut-mei=Mikio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SakaueTomohisa
en-aut-sei=Sakaue
en-aut-mei=Tomohisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HashimotoKohei
en-aut-sei=Hashimoto
en-aut-mei=Kohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NakataKentaro
en-aut-sei=Nakata
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ChoshiHaruki
en-aut-sei=Choshi
en-aut-mei=Haruki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TanakaShin
en-aut-sei=Tanaka
en-aut-mei=Shin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MiyoshiKentaroh
en-aut-sei=Miyoshi
en-aut-mei=Kentaroh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OhtaniShinji
en-aut-sei=Ohtani
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=OharaToshiaki
en-aut-sei=Ohara
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SugimotoSeiichiro
en-aut-sei=Sugimoto
en-aut-mei=Seiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=MatsukawaAkihiro
en-aut-sei=Matsukawa
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=ToyookaShinichi
en-aut-sei=Toyooka
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine
kn-affil=

affil-num=4
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Surgery, Division of Cardiovascular and Thoracic Surgery, Duke University School of Medicine
kn-affil=

affil-num=6
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University
kn-affil=

affil-num=10
en-affil=Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=lung transplantation
kn-keyword=lung transplantation
en-keyword=ischemia-reperfusion injury
kn-keyword=ischemia-reperfusion injury
en-keyword=donation after circulatory death
kn-keyword=donation after circulatory death
en-keyword=nuclear receptor subfamily 4 group A member 1
kn-keyword=nuclear receptor subfamily 4 group A member 1
en-keyword=endothelial cell
kn-keyword=endothelial cell
END

start-ver=1.4
cd-journal=joma
no-vol=136
cd-vols=
no-issue=3
article-no=
start-page=91
end-page=93
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20241202
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The 2023 Incentive Award of the Okayama Medical Association in General Medical Science (2023 Yuuki Prize)
kn-title=令和５年度岡山医学会賞　総合研究奨励賞（結城賞）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=WatanabeHaruki
en-aut-sei=Watanabe
en-aut-mei=Haruki
kn-aut-name=渡辺晴樹
kn-aut-sei=渡辺
kn-aut-mei=晴樹
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝学
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240925
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=非小細胞肺癌における腫瘍免疫状態の指標としての好中球リンパ球比の有用性
kn-title=Utility of neutrophil-to-lymphocyte ratio as an indicator of tumor immune status in non-small cell lung cancer
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=IWATAKazuma
en-aut-sei=IWATA
en-aut-mei=Kazuma
kn-aut-name=岩田一馬
kn-aut-sei=岩田
kn-aut-mei=一馬
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=19
cd-vols=
no-issue=10
article-no=
start-page=e0309622
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20241023
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The protective effect of carbamazepine on acute lung injury induced by hemorrhagic shock and resuscitation in rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hemorrhagic shock and resuscitation (HSR) enhances the risk of acute lung injury (ALI). This study investigated the protective effect of carbamazepine (CBZ) on HSR-induced ALI in rats. Male Sprague-Dawley rats were allocated into five distinct groups through randomization: control (SHAM), saline + HSR (HSR), CBZ + HSR (CBZ/HSR), dimethyl sulfoxide (DMSO) + HSR (DMSO/HSR), and CBZ + chloroquine (CQ) + HSR (CBZ/CQ/HSR). Subsequently, HSR models were established. To detect tissue damage, we measured lung histological changes, lung injury scores, and wet/dry weight ratios. We measured neutrophil counts as well as assessed the expression of inflammatory factors using RT-PCR to determine the inflammatory response. We detected autophagy-related proteins LC3II/LC3I, P62, Beclin-1, and Atg12-Atg5 using western blotting. Pretreatment with CBZ improved histopathological changes in the lungs and reduced lung injury scores. The CBZ pretreatment group exhibited significantly reduced lung wet/dry weight ratio, neutrophil aggregation and number, and inflammation factor (TNF-alpha and iNOS) expression. CBZ changed the expression levels of autophagy-related proteins (LC3II/LC3I, beclin-1, Atg12-Atg5, and P62), suggesting autophagy activation. However, after injecting CQ, an autophagy inhibitor, the beneficial effects of CBZ were reversed. Taken together, CBZ pretreatment improved HSR-induced ALI by suppressing inflammation, at least in part, through activating autophagy. Thus, our study offers a novel perspective for treating HSR-induced ALI.
en-copyright=
kn-copyright=

en-aut-name=LiYaqiang
en-aut-sei=Li
en-aut-mei=Yaqiang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShimizuHiroko
en-aut-sei=Shimizu
en-aut-mei=Hiroko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakamuraRyu
en-aut-sei=Nakamura
en-aut-mei=Ryu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=LuYifu
en-aut-sei=Lu
en-aut-mei=Yifu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SakamotoRisa
en-aut-sei=Sakamoto
en-aut-mei=Risa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OmoriEmiko
en-aut-sei=Omori
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakahashiToru
en-aut-sei=Takahashi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MorimatsuHiroshi
en-aut-sei=Morimatsu
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Anesthesiology and Resuscitology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Anesthesiology and Resuscitology, Okayama University Medical School
kn-affil=

affil-num=3
en-affil=Department of Anesthesiology and Resuscitology, Okayama University Medical School
kn-affil=

affil-num=4
en-affil=Department of Anesthesiology and Resuscitology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Anesthesiology and Resuscitology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Anesthesiology and Resuscitology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Okayama Saidaiji Hospital
kn-affil=

affil-num=8
en-affil=Department of Anesthesiology and Resuscitology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=1
article-no=
start-page=9869
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Absolute lymphocyte count and neutrophil-to-lymphocyte ratio as predictors of CDK 4/6 inhibitor efficacy in advanced breast cancer
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cyclin-dependent kinase 4 and 6 inhibitors (CDK4/6i) are the standard agents for treating patients with estrogen receptor-positive and human epidermal growth factor receptor 2-negative advanced breast cancer (ER + HER2 - ABC). However, markers predicting the outcomes of CDK4/6i treatment have yet to be identified. This study was a single-center retrospective cohort study. We retrospectively evaluated 101 patients with ER + HER2 - ABC receiving CDK4/6i in combination with endocrine therapy at Fukuyama City Hospital between November 2017 and July 2021. We investigated the clinical outcomes and the safety of CDK4/6i treatment, and the absolute lymphocyte count (ALC) and neutrophil-to-lymphocyte ratio (NLR) as predictive markers for CDK4/6i. We defined the cut-off values as 1000/mu L for ALC and 3 for NLR, and divided into "low" and "high" groups, respectively. We evaluated 43 and 58 patients who received abemaciclib and palbociclib, respectively. Patients with high ALC and low NLR had significantly longer overall survival than those with low ALC and high NLR (high vs. low; ALC: HR 0.29; 95% CI 0.12-0.70; NLR: HR 2.94; 95% CI 1.21-7.13). There was no significant difference in efficacy between abemaciclib and palbociclib and both had good safety profiles. We demonstrated that ALC and NLR might predict the outcomes of CDK4/6i treatment in patients with ER + HER2 - ABC.
en-copyright=
kn-copyright=

en-aut-name=NakamotoShogo
en-aut-sei=Nakamoto
en-aut-mei=Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShienTadahiko
en-aut-sei=Shien
en-aut-mei=Tadahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=IwamotoTakayuki
en-aut-sei=Iwamoto
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KuboShinichiro
en-aut-sei=Kubo
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YamamotoMari
en-aut-sei=Yamamoto
en-aut-mei=Mari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YamashitaTetsumasa
en-aut-sei=Yamashita
en-aut-mei=Tetsumasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KuwaharaChihiro
en-aut-sei=Kuwahara
en-aut-mei=Chihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IkedaMasahiko
en-aut-sei=Ikeda
en-aut-mei=Masahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital
kn-affil=

affil-num=4
en-affil=Department of Breast and Thyroid Surgery, Fukuyama City Hospital
kn-affil=

affil-num=5
en-affil=Department of Breast and Thyroid Surgery, Fukuyama City Hospital
kn-affil=

affil-num=6
en-affil=Department of Breast and Thyroid Surgery, Fukuyama City Hospital
kn-affil=

affil-num=7
en-affil=Department of Breast and Thyroid Surgery, Fukuyama City Hospital
kn-affil=

affil-num=8
en-affil=Department of Breast and Thyroid Surgery, Fukuyama City Hospital
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=10
article-no=
start-page=1811
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240509
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Impact of Nutritional Status on Neutrophil-to-Lymphocyte Ratio as a Predictor of Efficacy and Adverse Events of Immune Check-Point Inhibitors
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The neutrophil -to-lymphocyte ratio (NLR) is useful for predicting the effectiveness of treatment with immune checkpoint inhibitors (ICIs) and immune-related adverse events (irAEs). Because a growing body of evidence has recently shown that the number of lymphocytes that comprise NLR fluctuates according to nutritional status, this study examined whether the usefulness of NLR varies in ICI treatment due to changes in nutritional status. A retrospective analysis was performed on 1234 patients who received ICI treatment for malignant tumors at our hospital. Progression-free survival (PFS) was significantly prolonged in patients with NLR < 4. Multivariate analysis revealed that the factors associated with the occurrence of irAE were NLR < 4 and the use of ipilimumab. However, when limited to cases with serum albumin levels <3.8 g/dL, lymphocyte counts significantly decreased, and the associations between NLR and PFS and between NLR and irAE occurrence disappeared. In contrast, when limited to the cases with serum albumin levels ?3.8 g/dL, the associations remained, with significantly prolonged PFS and significantly increased irAE occurrence at NLR < 4. NLR may be a good predictive tool for PFS and irAE occurrence during ICI treatment when a good nutritional status is maintained.
en-copyright=
kn-copyright=

en-aut-name=SueMasahiko
en-aut-sei=Sue
en-aut-mei=Masahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakeuchiYasuto
en-aut-sei=Takeuchi
en-aut-mei=Yasuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HirataShoichiro
en-aut-sei=Hirata
en-aut-mei=Shoichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakakiAkinobu
en-aut-sei=Takaki
en-aut-mei=Akinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OtsukaMotoyuki
en-aut-sei=Otsuka
en-aut-mei=Motoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Gastroenterology and Hepatology, Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Department of Gastroenterology and Hepatology, Okayama University Hospital
kn-affil=

affil-num=4
en-affil=Department of Gastroenterology and Hepatology, Okayama University Hospital
kn-affil=

affil-num=5
en-affil=Department of Gastroenterology and Hepatology, Okayama University Hospital
kn-affil=
en-keyword=immune-related adverse events
kn-keyword=immune-related adverse events
en-keyword=serum albumin
kn-keyword=serum albumin
en-keyword=real-world practice
kn-keyword=real-world practice
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=二重抗血小板療法は好中球細胞外トラップを阻害することで、肝内胆管癌の微小肝転移を抑制する
kn-title=Dual antiplatelet therapy inhibits neutrophil extracellular traps to reduce liver micrometastases of intrahepatic cholangiocarcinoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=YOSHIMOTOMasashi
en-aut-sei=YOSHIMOTO
en-aut-mei=Masashi
kn-aut-name=吉本匡志
kn-aut-sei=吉本
kn-aut-mei=匡志
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=78
cd-vols=
no-issue=2
article-no=
start-page=95
end-page=106
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=202404
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Roles of Neuropeptide Y in Respiratory Disease Pathogenesis via the Airway Immune Response
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The lungs are very complex organs, and the respiratory system performs the dual roles of repairing tissue while protecting against infection from various environmental stimuli. Persistent external irritation disrupts the immune responses of tissues and cells in the respiratory system, ultimately leading to respiratory disease. Neuropeptide Y (NPY) is a 36-amino-acid polypeptide and a neurotransmitter that regulates homeostasis. The NPY receptor is a seven-transmembrane-domain G-protein-coupled receptor with six subtypes (Y1, Y2, Y3, Y4, Y5, and Y6). Of these receptors, Y1, Y2, Y4, and Y5 are functional in humans, and Y1 plays important roles in the immune responses of many organs, including the respiratory system. NPY and the Y1 receptor have critical roles in the pathogenesis of asthma, chronic obstructive pulmonary disease, and idiopathic pulmonary fibrosis. The effects of NPY on the airway immune response and pathogenesis differ among respiratory diseases. This review focuses on the involvement of NPY in the airway immune response and pathogenesis of various respiratory diseases.
en-copyright=
kn-copyright=

en-aut-name=ItanoJunko
en-aut-sei=Itano
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KiuraKatsuyuki
en-aut-sei=Kiura
en-aut-mei=Katsuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MaedaYoshinobu
en-aut-sei=Maeda
en-aut-mei=Yoshinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MiyaharaNobuaki
en-aut-sei=Miyahara
en-aut-mei=Nobuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Allergy and Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Allergy and Respiratory Medicine, Okayama University Hospital
kn-affil=
en-keyword=neuropeptide y
kn-keyword=neuropeptide y
en-keyword=Y1 receptor
kn-keyword=Y1 receptor
en-keyword=airway immune response
kn-keyword=airway immune response
en-keyword=bronchial epithelial cells
kn-keyword=bronchial epithelial cells
en-keyword=respiratory disease
kn-keyword=respiratory disease
END

start-ver=1.4
cd-journal=joma
no-vol=135
cd-vols=
no-issue=3
article-no=
start-page=136
end-page=141
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20231201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Neutrophil dynamics in renal ischemia-reperfusion injury
kn-title=腎虚血再灌流障害における好中球動態
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=ArakiMotoo
en-aut-sei=Araki
en-aut-mei=Motoo
kn-aut-name=荒木元朗
kn-aut-sei=荒木
kn-aut-mei=元朗
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Urology, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama University
kn-affil=岡山大学学術研究院医歯薬学域　泌尿器病態学
en-keyword=腎虚血再灌流障害
kn-keyword=腎虚血再灌流障害
en-keyword=リアルタイムイメージング
kn-keyword=リアルタイムイメージング
en-keyword=好中球
kn-keyword=好中球
en-keyword=腎移植
kn-keyword=腎移植
END

start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=13
article-no=
start-page=8727
end-page=8734
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230901
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Prognostic Impact of Tumor-Infiltrating Lymphocytes, Tertiary Lymphoid Structures, and Neutrophil-to-Lymphocyte Ratio in Pulmonary Metastases from Uterine Leiomyosarcoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background　The presence of tumor-infiltrating lymphocytes (TILs) and tertiary lymphoid structures (TLSs) in tumor tissue has been related to the prognosis in various malignancies. Meanwhile, neutrophil-to-lymphocyte ratio (NLR) as a systemic inflammation marker also has been associated with the prognosis in them. However, few reports have investigated the relationship between pulmonary metastases from sarcoma and these biomarkers.<br>
Methods　We retrospectively recruited 102 patients undergoing metastasectomy for pulmonary metastases from uterine leiomyosarcoma at Okayama University Hospital from January 2006 to December 2019. TILs and TLSs were evaluated by immunohistochemical staining of surgically resected specimens of pulmonary metastases using anti-CD3/CD8/CD103/Foxp3/CD20 antibodies. NLR was calculated from the blood examination immediately before the most recent pulmonary metastasectomy. We elucidated the relationship between the prognosis and these factors. Because we considered that the status of tumor tissue and systemic inflammation were equally valuable, we also assessed the impact of the combination of TILs or TLSs and NLR on the prognosis.<br>
Results　As for TILs, CD3-positive cells and CD8-positive cells were correlated with the prognosis. The prognosis was significantly better in patients with CD3-high group, CD8-high group, TLSs-high group, and NLR-low group, respectively. The prognosis of CD8-high/NLR-low group and TLSs-high/NLR-low group was significantly better than that of CD8-low/NLR-high group and TLSs-low/NLR-high group, respectively.<br>
Conclusions　CD3-positive TILs, CD8-positive TILs, TLSs, and NLR are correlated with the prognosis, respectively. The combination of CD8-positive TILs or TLSs and NLR may be the indicators to predict the prognosis of patients with pulmonary metastases from uterine leiomyosarcoma.
en-copyright=
kn-copyright=

en-aut-name=MatsudaNaoki
en-aut-sei=Matsuda
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamamotoHiromasa
en-aut-sei=Yamamoto
en-aut-mei=Hiromasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HabuTomohiro
en-aut-sei=Habu
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IwataKazuma
en-aut-sei=Iwata
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MatsubaraKei
en-aut-sei=Matsubara
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanakaShin
en-aut-sei=Tanaka
en-aut-mei=Shin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HashimotoKohei
en-aut-sei=Hashimoto
en-aut-mei=Kohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ShienKazuhiko
en-aut-sei=Shien
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SuzawaKen
en-aut-sei=Suzawa
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MiyoshiKentaroh
en-aut-sei=Miyoshi
en-aut-mei=Kentaroh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=TojiTomohiro
en-aut-sei=Toji
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=OkazakiMikio
en-aut-sei=Okazaki
en-aut-mei=Mikio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=SugimotoSeiichiro
en-aut-sei=Sugimoto
en-aut-mei=Seiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=TakahashiKatsuhito
en-aut-sei=Takahashi
en-aut-mei=Katsuhito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=ToyookaShinichi
en-aut-sei=Toyooka
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

affil-num=1
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Department of Sarcoma Medicine, Center for Sarcoma Multidisciplinary Treatment, Kameda Medical Center
kn-affil=

affil-num=15
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=62
cd-vols=
no-issue=15
article-no=
start-page=2215
end-page=2221
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230801
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Co-occurrence of Three Systemic Diseases: ANCA-associated Vasculitis, Sjogren's syndrome and Sarcoidosis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), Sjogren's syndrome (SjS), and sarcoidosis are systemic diseases targeting multiple organs. While a careful differential diagnosis of these dis-eases is often required, their co-occurrence in the same patient has been previously reported. We herein report a 58-year-old Japanese man diagnosed with the co-occurrence of three systemic diseases (AAV, SjS, and sar-coidosis) in addition to monoclonal gammopathy of undetermined significance (MGUS), which emphasizes the importance of considering the possible co-occurrence of these diseases as well as their differentiation.
en-copyright=
kn-copyright=

en-aut-name=TsujiKenji
en-aut-sei=Tsuji
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkuyamaYuka
en-aut-sei=Okuyama
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AsanoYosuke
en-aut-sei=Asano
en-aut-mei=Yosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YamaokaKimitomo
en-aut-sei=Yamaoka
en-aut-mei=Kimitomo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KitamuraShinji
en-aut-sei=Kitamura
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Academic Field of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Academic Field of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Academic Field of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Academic Field of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Academic Field of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Academic Field of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=ANCA-associated vasculitis
kn-keyword=ANCA-associated vasculitis
en-keyword=Sjogren's syndrome
kn-keyword=Sjogren's syndrome
en-keyword=sarcoidosis
kn-keyword=sarcoidosis
en-keyword=monoclonal gammopathy of undetermined significance
kn-keyword=monoclonal gammopathy of undetermined significance
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=4
article-no=
start-page=377
end-page=385
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=202308
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Disease Progression-Related Markers for Aged Non-Alcoholic Fatty Liver Disease Patients
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Liver fibrosis is an important phenomenon in non-alcoholic fatty liver disease (NAFLD) progression. Standard markers reflecting liver fibrosis, including the FIB-4 index, increase with age. This study aimed to identify fibrosis progression-related markers that are diagnostically beneficial even in aged individuals. Serum levels of pro- and anti-inflammatory cytokines were measured by multiple enzyme-linked immunosorbent assay. Two standard NAFLD or fibrosis progression-related markers ? the FIB-4 index and APRI score ? were analyzed along with cytokine levels to define the best approach to discriminate advanced fibrosis. Ninety-eight NAFLD patients were enrolled: 59 and 39 patients with fibrosis stages 1-2 and 3-4 respectively. In addition to the FIB-4 index and APRI score, the following factors showed significant differences between stages 1-2 and stages 3-4 in a multivariate analysis: platelet counts, IP-10, and RANTES. The fibrosis stage, FIB-4, APRI, PDGF-BB, and RANTES were related to the prognosis. In aged patients, IP-10, GM-CSF, and RANTES differed between stages 1-2 and stages 3-4. FIB-4 and APRI were beneficial for their correlation with fibrosis. However, to stratify either young or elderly advanced fibrosis patients, and to identify patients likely to have a bad outcome, RANTES was the best marker.
en-copyright=
kn-copyright=

en-aut-name=MorimotoKosaku
en-aut-sei=Morimoto
en-aut-mei=Kosaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakeuchiYasuto
en-aut-sei=Takeuchi
en-aut-mei=Yasuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakakiAkinobu
en-aut-sei=Takaki
en-aut-mei=Akinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WadaNozomu
en-aut-sei=Wada
en-aut-mei=Nozomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OyamaAtsushi
en-aut-sei=Oyama
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AdachiTakuya
en-aut-sei=Adachi
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OnishiHideki
en-aut-sei=Onishi
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ShirahaHidenori
en-aut-sei=Shiraha
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=NAFLD
kn-keyword=NAFLD
en-keyword=NASH
kn-keyword=NASH
en-keyword=liver fibrosis
kn-keyword=liver fibrosis
en-keyword=chemokine
kn-keyword=chemokine
en-keyword=FIB-4
kn-keyword=FIB-4
END

start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=12
article-no=
start-page=1927
end-page=1949
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20231201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Enhanced Production of EPA-Derived Anti-Inflammatory Metabolites after Oral Administration of a Novel Self-Emulsifying Highly Purified EPA Ethyl Ester Formulation (MND-2119)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aims: MND-2119 is a novel once-daily dose self-emulsifying formulation of highly purified eicosapentaenoic acid ethyl ester (EPA-E) and is approved as an antihyperlipidemia agent in Japan. It has improved absorption and achieves higher plasma EPA concentrations at Cmax than conventional EPA-E. In the JELIS trial, concomitant use of EPA-E with statin therapy significantly reduced atherosclerotic cardiovascular disease (ASCVD) risks. As a potential mechanism of action of EPA, endogenous formation of EPA-derived anti-inflammatory metabolites is receiving greater attention. This study aims to investigate the endogenous formation of EPA-derived anti-inflammatory metabolites following single and multiple administrations of MND-2119.<br>
Methods: Healthy adult male subjects were randomly assigned to a nonintervention (control) group, MND-2119 2-g/day group, MND-2119 4-g/day group, or EPA-E 1.8-g/day group for 7 days (N=8 per group). Plasma fatty acids and EPA-derived metabolites were evaluated. Peripheral blood neutrophils were isolated, and the production of EPA-derived metabolites from in vitro stimulated neutrophils was evaluated.<br>
Results: After single and multiple administrations of MND-2119 2 g/day, there were significant increases in plasma EPA concentration, 18-hydroxyeicosapentaenoic acid (18-HEPE), and 17,18-epoxyeicosatetraenoic acid compared with those of EPA-E 1.8 g/day. They were further increased with MND-2119 4 g/day administration. In neutrophils, the EPA concentration in the MND-2119 2-g/day group was significantly higher compared with that in the EPA-E 1.8-g/day group after multiple administration, and 18-HEPE production was positively correlated with EPA concentration. No safety issues were noted.<br>
Conclusions: These results demonstrate that MND-2119 increases the plasma and cellular concentrations of EPA and EPA-derived metabolites to a greater extent than conventional EPA-E formulations.
en-copyright=
kn-copyright=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NaoeSatoko
en-aut-sei=Naoe
en-aut-mei=Satoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WakabayashiHiroyuki
en-aut-sei=Wakabayashi
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YanoTakashi
en-aut-sei=Yano
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MoriTakuya
en-aut-sei=Mori
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KandaShingo
en-aut-sei=Kanda
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=AritaMakoto
en-aut-sei=Arita
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Medical Affairs Department, Mochida Pharmaceutical Co., Ltd.
kn-affil=

affil-num=3
en-affil=Medical Affairs Department, Mochida Pharmaceutical Co., Ltd.
kn-affil=

affil-num=4
en-affil=Medical Affairs Department, Mochida Pharmaceutical Co., Ltd.
kn-affil=

affil-num=5
en-affil=Clinical Research Department, Mochida Pharmaceutical Co., Ltd.
kn-affil=

affil-num=6
en-affil=Clinical Development Planning and Management Department, Mochida Pharmaceutical Co., Ltd.
kn-affil=

affil-num=7
en-affil=Laboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences
kn-affil=

affil-num=8
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Eicosapentaenoic acid
kn-keyword=Eicosapentaenoic acid
en-keyword=MND-2119
kn-keyword=MND-2119
en-keyword=Metablolite
kn-keyword=Metablolite
en-keyword=Inflammation
kn-keyword=Inflammation
END

start-ver=1.4
cd-journal=joma
no-vol=567
cd-vols=
no-issue=
article-no=
start-page=216260
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230728
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Dual antiplatelet therapy inhibits neutrophil extracellular traps to reduce liver micrometastases of intrahepatic cholangiocarcinoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The involvement of neutrophil extracellular traps (NETs) in cancer metastasis is being clarified, but the relationship between intrahepatic cholangiocarcinoma (iCCA) and NETs remains unclear. The presence of NETs was verified by multiple fluorescence staining in clinically resected specimens of iCCA. Human neutrophils were co-cultured with iCCA cells to observe NET induction and changes in cellular characteristics. Binding of platelets to iCCA cells and its mechanism were also examined, and their effects on NETs were analyzed in vitro and in in vivo mouse models. NETs were present in the tumor periphery of resected iCCAs. NETs promoted the motility and migration ability of iCCA cells in vitro. Although iCCA cells alone had a weak NET-inducing ability, the binding of platelets to iCCA cells via P-selectin promoted NET induction. Based on these results, antiplatelet drugs were applied to these cocultures in vitro and inhibited the binding of platelets to iCCA cells and the induction of NETs. Fluorescently labeled iCCA cells were injected into the spleen of mice, resulting in the formation of liver micrometastases coexisting with platelets and NETs. These mice were treated with dual antiplatelet therapy (DAPT) consisting of aspirin and ticagrelor, which dramatically reduced micrometastases. These results suggest that potent antiplatelet therapy prevents micrometastases of iCCA cells by inhibiting platelet activation and NET production, and it may contribute to a novel therapeutic strategy.
en-copyright=
kn-copyright=

en-aut-name=YoshimotoMasashi
en-aut-sei=Yoshimoto
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KagawaShunsuke
en-aut-sei=Kagawa
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KajiokaHiroki
en-aut-sei=Kajioka
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TaniguchiAtsuki
en-aut-sei=Taniguchi
en-aut-mei=Atsuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KurodaShinji
en-aut-sei=Kuroda
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KikuchiSatoru
en-aut-sei=Kikuchi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KakiuchiYoshihiko
en-aut-sei=Kakiuchi
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YagiTomohiko
en-aut-sei=Yagi
en-aut-mei=Tomohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NogiShohei
en-aut-sei=Nogi
en-aut-mei=Shohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=TeraishiFuminori
en-aut-sei=Teraishi
en-aut-mei=Fuminori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=ShigeyasuKunitoshi
en-aut-sei=Shigeyasu
en-aut-mei=Kunitoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=YoshidaRyuichi
en-aut-sei=Yoshida
en-aut-mei=Ryuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=UmedaYuzo
en-aut-sei=Umeda
en-aut-mei=Yuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=NomaKazuhiro
en-aut-sei=Noma
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=TazawaHiroshi
en-aut-sei=Tazawa
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

affil-num=1
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Aspirin
kn-keyword=Aspirin
en-keyword=Ticagrelor
kn-keyword=Ticagrelor
en-keyword=P-selectin
kn-keyword=P-selectin
en-keyword=Platelet
kn-keyword=Platelet
en-keyword=Time-lapse imaging
kn-keyword=Time-lapse imaging
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230324
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=集学的治療が行われた局所進行肺癌患者における末梢血好中球/リンパ球比（NLR)の予後的意義について
kn-title=Prognostic Signi?cance of Neutrophil-to-Lymphocyte Ratio in Locally Advanced Non-small-cell Lung Cancer Treated with Trimodality Therapy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=TSUDAKAShimpei
en-aut-sei=TSUDAKA
en-aut-mei=Shimpei
kn-aut-name=津��慎平
kn-aut-sei=津��
kn-aut-mei=慎平
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=1
article-no=
start-page=37
end-page=43
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=202302
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Efficacy of Inflammatory and Immune Markers for Predicting the Prognosis of Patients with Stage IV Breast Cancer
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Systemic therapy for stage IV breast cancer is usually an initial treatment and is based on findings regarding biomarkers (e.g., hormone receptors and human epidermal growth factor receptor-2 [HER2]). However, the response to therapy and outcomes sometime differ among patients with similar prognostic factors including grade, hormone receptor, HER2, and more. We conducted retrospective analyses to evaluate the correlations between the overall survival (OS) of 46 stage IV breast cancer patients and (i) the peripheral absolute lymphocyte count (ALC) and (ii) composite blood cell markers. The peripheral blood cell markers included the neutrophil- to-lymphocyte ratio (NLR), the monocyte-to-lymphocyte ratio (MLR), the systemic immune-inflammation index (SII), the systemic inflammation response index (SIRI), and the most recently introduced indicator, the pan-immune-inflammatory value (PIV). The SIRI and PIV showed prognostic impacts on the patients: those with a low SIRI or a low PIV showed significantly better OS than those with a high SIRI (5-year, 66.0% vs. 35.0%, p<0.05) or high PIV (5-year, 68.1% vs. 38.5%, p<0.05), respectively. This is the first report indicating the possible prognostic value of the PIV for OS in patients with stage IV breast cancer. Further studies with larger numbers of patients are necessary for further clarification.
en-copyright=
kn-copyright=

en-aut-name=YamanouchiKosho
en-aut-sei=Yamanouchi
en-aut-mei=Kosho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MaedaShigeto
en-aut-sei=Maeda
en-aut-mei=Shigeto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Department of Surgery, National Hospital Organization, Nagasaki Medical Center
kn-affil=

affil-num=2
en-affil=Department of Surgery, National Hospital Organization, Nagasaki Medical Center
kn-affil=
en-keyword=breast cancer
kn-keyword=breast cancer
en-keyword=pan-immune-inflammatory value
kn-keyword=pan-immune-inflammatory value
en-keyword=prognosis
kn-keyword=prognosis
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=1
article-no=
start-page=110
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230110
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Extracellular Vesicles: New Classification and Tumor Immunosuppression
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Simple Summary Extracellular vesicles (EVs) are cell-derived membrane-surrounded vesicles that carry bioactive molecules and deliver them to recipient cells. Classical EVs are exosomes, microvesicles, and apoptotic bodies. This review classifies classical and additional EV types, including autophagic EVs, matrix vesicles, and stressed EVs. Of note, matrix vesicles are key components interacting with extracellular matrices (ECM) in the tumor microenvironment. We also review how EVs are involved in the communication between cancer cells and tumor-associated cells (TAC), leading to establishing immunosuppressive and chemoresistant microenvironments. These include cancer-associated fibroblasts (CAF), mesenchymal stem cells (MSC), blood endothelial cells (BEC), lymph endothelial cells (LEC), and immune cells, such as tumor-associated macrophages (TAM), tumor-associated neutrophils (TAN), dendritic cells, natural killer cells, killer T cells, and immunosuppressive cells, such as regulatory T cells and myeloid-derived suppressor cells (MDSC). Exosomal long noncoding RNA (lncRNA), microRNA, circular RNA, piRNA, mRNA, and proteins are crucial in communication between cancer cells and TACs for establishing cold tumors. Extracellular vesicles (EVs) are cell-derived membrane-surrounded vesicles carrying various types of molecules. These EV cargoes are often used as pathophysiological biomarkers and delivered to recipient cells whose fates are often altered in local and distant tissues. Classical EVs are exosomes, microvesicles, and apoptotic bodies, while recent studies discovered autophagic EVs, stressed EVs, and matrix vesicles. Here, we classify classical and new EVs and non-EV nanoparticles. We also review EVs-mediated intercellular communication between cancer cells and various types of tumor-associated cells, such as cancer-associated fibroblasts, adipocytes, blood vessels, lymphatic vessels, and immune cells. Of note, cancer EVs play crucial roles in immunosuppression, immune evasion, and immunotherapy resistance. Thus, cancer EVs change hot tumors into cold ones. Moreover, cancer EVs affect nonimmune cells to promote cellular transformation, including epithelial-to-mesenchymal transition (EMT), chemoresistance, tumor matrix production, destruction of biological barriers, angiogenesis, lymphangiogenesis, and metastatic niche formation.
en-copyright=
kn-copyright=

en-aut-name=ShetaMona
en-aut-sei=Sheta
en-aut-mei=Mona
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TahaEman A.
en-aut-sei=Taha
en-aut-mei=Eman A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=LuYanyin
en-aut-sei=Lu
en-aut-mei=Yanyin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=EguchiTakanori
en-aut-sei=Eguchi
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Dental Pharmacology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Biochemistry, Faculty of Science, Ain Shams University
kn-affil=

affil-num=3
en-affil=Department of Dental Pharmacology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Dental Pharmacology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=extracellular vesicle
kn-keyword=extracellular vesicle
en-keyword=exosome
kn-keyword=exosome
en-keyword=autophagy
kn-keyword=autophagy
en-keyword=amphisome
kn-keyword=amphisome
en-keyword=matrix vesicle
kn-keyword=matrix vesicle
en-keyword=cellular communication
kn-keyword=cellular communication
en-keyword=tumor microenvironment
kn-keyword=tumor microenvironment
en-keyword=immunosuppression
kn-keyword=immunosuppression
en-keyword=immune evasion
kn-keyword=immune evasion
en-keyword=therapy resistance
kn-keyword=therapy resistance
END

start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=12
article-no=
start-page=2495
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221216
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whether the nitrate-reducing bacteria isolated from patients with atrophic gastritis caused by H. pylori infection are risk factors for developing atrophic gastritis and gastric neoplasia. Nitrate-reducing bacteria were isolated from patients with atrophic gastritis caused by H. pylori infection. Among the isolated bacteria, Actinomyces oris, Actinomyces odontolyticus, Rothia dentocariosa, and Rothia mucilaginosa were used in the subsequent experiments. Cytokine inducibility was evaluated in monocytic cells, and mitogen-activated protein kinase (MAPK) activity and cell cycle were assessed in the gastric epithelial cells. The cytotoxicities and neutrophil-inducing abilities of the Actinomyces and Rothia species were enhanced when cocultured with H. pylori. Th1/Th2-related cytokines were also expressed, but their expression levels differed depending on the bacterial species. Moreover, H. pylori and Actinomyces activated MAPK (ERK and p38) and affected cell cycle progression. Some nitrate-reducing bacteria cocultured with H. pylori may promote inflammation and atrophy by inducing cytokine production. In addition, the MAPK activation and cell cycle progression caused by these bacteria can contribute to gastric cancer development.
en-copyright=
kn-copyright=

en-aut-name=OjimaHinako
en-aut-sei=Ojima
en-aut-mei=Hinako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KuraokaSakiko
en-aut-sei=Kuraoka
en-aut-mei=Sakiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OkanoueShyoutarou
en-aut-sei=Okanoue
en-aut-mei=Shyoutarou
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=GotohKazuyoshi
en-aut-sei=Gotoh
en-aut-mei=Kazuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MatsushitaOsamu
en-aut-sei=Matsushita
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=WatanabeAkari
en-aut-sei=Watanabe
en-aut-mei=Akari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YokotaKenji
en-aut-sei=Yokota
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Bacteriology, Academic Field of Health Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Gastroenterology and Hepatology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Gastroenterology and Hepatology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Himeji Red Cross Hospital
kn-affil=

affil-num=5
en-affil=Department of Bacteriology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Bacteriology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Oral Health Care and Rehabilitation, Institute of Biomedical Sciences, Graduate School, Tokushima University
kn-affil=

affil-num=8
en-affil=Department of Bacteriology, Academic Field of Health Sciences, Okayama University
kn-affil=
en-keyword=Helicobacter pylori
kn-keyword=Helicobacter pylori
en-keyword=nitrate-reducing bacteria
kn-keyword=nitrate-reducing bacteria
en-keyword=IL-8
kn-keyword=IL-8
en-keyword=TNF-alpha
kn-keyword=TNF-alpha
en-keyword=cell cycle
kn-keyword=cell cycle
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=11
article-no=
start-page=673
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221110
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Functional Blockage of S100A8/A9 Ameliorates Ischemia-Reperfusion Injury in the Lung
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=(1) Background: Lung ischemia-reperfusion (IR) injury increases the mortality and morbidity of patients undergoing lung transplantation. The objective of this study was to identify the key initiator of lung IR injury and to evaluate pharmacological therapeutic approaches using a functional inhibitor against the identified molecule. (2) Methods: Using a mouse hilar clamp model, the combination of RNA sequencing and histological investigations revealed that neutrophil-derived S100A8/A9 plays a central role in inflammatory reactions during lung IR injury. Mice were assigned to sham and IR groups with or without the injection of anti-S100A8/A9 neutralizing monoclonal antibody (mAb). (3) Results: Anti-S100A8/A9 mAb treatment significantly attenuated plasma S100A8/A9 levels compared with control IgG. As evaluated by oxygenation capacity and neutrophil infiltration, the antibody treatment dramatically ameliorated the IR injury. The gene expression levels of cytokines and chemokines induced by IR injury were significantly reduced by the neutralizing antibody. Furthermore, the antibody treatment significantly reduced TUNEL-positive cells, indicating the presence of apoptotic cells. (4) Conclusions: We identified S100A8/A9 as a novel therapeutic target against lung IR injury.
en-copyright=
kn-copyright=

en-aut-name=NakataKentaro
en-aut-sei=Nakata
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkazakiMikio
en-aut-sei=Okazaki
en-aut-mei=Mikio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SakaueTomohisa
en-aut-sei=Sakaue
en-aut-mei=Tomohisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KinoshitaRie
en-aut-sei=Kinoshita
en-aut-mei=Rie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KomodaYuhei
en-aut-sei=Komoda
en-aut-mei=Yuhei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ShimizuDai
en-aut-sei=Shimizu
en-aut-mei=Dai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YamamotoHaruchika
en-aut-sei=Yamamoto
en-aut-mei=Haruchika
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TanakaShin
en-aut-sei=Tanaka
en-aut-mei=Shin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SuzawaKen
en-aut-sei=Suzawa
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ShienKazuhiko
en-aut-sei=Shien
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=MiyoshiKentaroh
en-aut-sei=Miyoshi
en-aut-mei=Kentaroh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=YamamotoHiromasa
en-aut-sei=Yamamoto
en-aut-mei=Hiromasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=OharaToshiaki
en-aut-sei=Ohara
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=SugimotoSeiichiro
en-aut-sei=Sugimoto
en-aut-mei=Seiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=YamaneMasaomi
en-aut-sei=Yamane
en-aut-mei=Masaomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=MatsukawaAkihiro
en-aut-sei=Matsukawa
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=SakaguchiMasakiyo
en-aut-sei=Sakaguchi
en-aut-mei=Masakiyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=ToyookaShinichi
en-aut-sei=Toyooka
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

affil-num=1
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine
kn-affil=

affil-num=4
en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine
kn-affil=

affil-num=6
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network
kn-affil=

affil-num=8
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil= Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=17
en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=18
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=ischemia reperfusion injury
kn-keyword=ischemia reperfusion injury
en-keyword= S100A8/A9
kn-keyword= S100A8/A9
en-keyword=lung transplantation
kn-keyword=lung transplantation
en-keyword=damage-associated molecule patterns
kn-keyword=damage-associated molecule patterns
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=5
article-no=
start-page=585
end-page=591
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=202210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Surgery Outcomes for Pulmonary Metastases from Renal Cell Carcinoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Pulmonary metastatic resection is a standard therapy for renal cell carcinoma (RCC). Although patients with pulmonary metastases who do not undergo any treatment have poor prognoses, it has been reported that resection for pulmonary metastases yields good clinical outcomes. We investigated the prognoses of the 10 Japanese patients (eight males, two females) who underwent a surgical resection of pulmonary metastasectomy from RCC at our institution between April 1, 2012 and March 31, 2020 and analyzed the prognostic factors. We determined the prognoses and calculated the 5-year overall survival (OS) and disease-free survival (DFS) rates. To identify prognostic factors, we compared the median DFS duration for each factor. Elderly patients (median age, 75.5 years) were more predominant compared to previous studies, and all 10 patients underwent a complete resection. The 5-year DFS rate was 30.5% (95%CI: 0.045-0.63) and the 5-year OS rate was 80% (95%CI: 0.20-0.97). The following factors were associated with better prognosis: female, disease-free interval?36 months, and metastases size<12 mm. These results indicate that complete resection for pulmonary metastases from RCC resulted in good clinical outcomes, particularly for patients with better prognostic factors.
en-copyright=
kn-copyright=

en-aut-name=ChoshiHaruki
en-aut-sei=Choshi
en-aut-mei=Haruki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WatanabeMototsugu
en-aut-sei=Watanabe
en-aut-mei=Mototsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FurukawaShinichi
en-aut-sei=Furukawa
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=UjikeHiroyuki
en-aut-sei=Ujike
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KataokaKazuhiko
en-aut-sei=Kataoka
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Department of Thoracic Surgery, National Hospital Organization Iwakuni Clinical Center
kn-affil=

affil-num=2
en-affil=Department of Thoracic Surgery, National Hospital Organization Iwakuni Clinical Center
kn-affil=

affil-num=3
en-affil=Department of Thoracic Surgery, National Hospital Organization Iwakuni Clinical Center
kn-affil=

affil-num=4
en-affil=Department of Thoracic Surgery, National Hospital Organization Iwakuni Clinical Center
kn-affil=

affil-num=5
en-affil=Department of Thoracic Surgery, National Hospital Organization Iwakuni Clinical Center
kn-affil=
en-keyword=renal cell carcinoma
kn-keyword=renal cell carcinoma
en-keyword=pulmonary metastasis
kn-keyword=pulmonary metastasis
en-keyword=complete resection
kn-keyword=complete resection
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=5
article-no=
start-page=489
end-page=502
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=202210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Current Insights into Mesenchymal Signatures in Glioblastoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Glioblastoma (GBM) is a fatal primary malignant brain tumor in adults. Despite decades of research, the prognosis for GBM patients is still disappointing. One major reason for the intense therapeutic resistance of GBM is inter- and intra-tumor heterogeneity. GBM-intrinsic transcriptional profiling has suggested the presence of at least three subtypes of GBM: the proneural, classic, and mesenchymal subtypes. The mesenchymal subtype is the most aggressive, and patients with the mesenchymal subtype of primary and recurrent tumors tend to have a worse prognosis compared with patients with the other subtypes. Furthermore, GBM can shift from other subtypes to the mesenchymal subtype over the course of disease progression or recurrence. This phenotypic transition is driven by diverse tumor-intrinsic molecular mechanisms or microenvironmental factors. Thus, better understanding of the plastic nature of mesenchymal transition in GBM is pivotal to developing new therapeutic strategies. In this review, we provide a comprehensive overview of the current understanding of the elements involved in the mesenchymal transition of GBM and discuss future perspectives.
en-copyright=
kn-copyright=

en-aut-name=MatsumotoYuji
en-aut-sei=Matsumoto
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IchikawaTomotsugu
en-aut-sei=Ichikawa
en-aut-mei=Tomotsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurozumiKazuhiko
en-aut-sei=Kurozumi
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=DateIsao
en-aut-sei=Date
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Neurological Surgery, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=3
en-affil=Department of Neurosurgery, Hamamatsu University Hospital
kn-affil=

affil-num=4
en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=glioma
kn-keyword=glioma
en-keyword=glioblastoma
kn-keyword=glioblastoma
en-keyword=mesenchymal subtype
kn-keyword=mesenchymal subtype
en-keyword=mesenchymal transition
kn-keyword=mesenchymal transition
en-keyword=heterogeneity
kn-keyword=heterogeneity
END

start-ver=1.4
cd-journal=joma
no-vol=150
cd-vols=
no-issue=1
article-no=
start-page=9
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=202209
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Novel aspects of sepsis pathophysiology: NETs, plasma glycoproteins, endotheliopathy and COVID-19
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In 2016, sepsis was newly defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Sepsis remains one of the crucial medical problems to be solved worldwide. Although the world health organization has made sepsis a global health priority, there remain no specific and effective therapy for sepsis so far. Indeed, over the previous decades almost all attempts to develop novel drugs have failed. This may be partly ascribable to the multifactorial complexity of the septic cascade and the resultant difficulties of identifying drug targets. In addition, there might still be missing links among dysregulated host responses in vital organs. In this review article, recent advances in understanding of the complex pathophysiology of sepsis are summarized, with a focus on neutrophil extracellular traps (NETs), the significant role of NETs in thrombosis/embolism, and the functional roles of plasma proteins, histidine-rich glycoprotein (HRG) and inter-alpha-inhibitor proteins (IAIPs). The specific plasma proteins that are markedly decreased in the acute phase of sepsis may play important roles in the regulation of blood cells, vascular endothelial cells and coagulation. The accumulating evidence may provide us with insights into a novel aspect of the pathophysiology of sepsis and septic ARDS, including that in COVID-19. 
en-copyright=
kn-copyright=

en-aut-name=NishiboriM.
en-aut-sei=Nishibori
en-aut-mei=M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Translational Research and Drug Development, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Sepsis
kn-keyword=Sepsis
en-keyword=Histidine-rich glycoprotein (HRG)
kn-keyword=Histidine-rich glycoprotein (HRG)
en-keyword=Neutrophil extracellular traps (NETs)
kn-keyword=Neutrophil extracellular traps (NETs)
en-keyword=Endotheliopathy
kn-keyword=Endotheliopathy
en-keyword=COVID-19
kn-keyword=COVID-19
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210924
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=腎虚血再灌流におけるタダラフィルの保護作用によってもたらされる好中球動態変化のリアルタイムイメージングを用いた解析
kn-title=Evaluation of Neutrophil Dynamics Change by Protective Effect of Tadalafil After Rena Ischemia/Reperfusion Using In Vivo Real-time Imaging
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=MaruyamaYuki
en-aut-sei=Maruyama
en-aut-mei=Yuki
kn-aut-name=丸山雄樹
kn-aut-sei=丸山
kn-aut-mei=雄樹
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=6
article-no=
start-page=671
end-page=675
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202112
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Multiple Roles of Histidine-Rich Glycoprotein in Vascular Homeostasis and Angiogenesis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Histidine-rich glycoprotein (HRG) is a 75 kDa plasma protein that is synthesized in the liver of many verte-brates and present in their plasma at relatively high concentrations of 100-150 μg/mL. HRG is an abundant and well-characterized protein having a multidomain structure that enable it to interact with many ligands, func-tion as an adaptor molecule, and participate in numerous physiological and pathological processes. As a plasma protein, HRG has been reported to regulate vascular biology, including coagulation, fibrinolysis and angiogenesis, through its binding with several ligands (heparin, FXII, fibrinogen, thrombospondin, and plas-minogen) and interaction with many types of cells (endothelial cells, erythrocytes, neutrophils and platelets). This review aims to summarize the roles of HRG in maintaining vascular homeostasis and regulating angiogen-esis in various pathological conditions.
en-copyright=
kn-copyright=

en-aut-name=GaoShangze
en-aut-sei=Gao
en-aut-mei=Shangze
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NishiboriMasahiro
en-aut-sei=Nishibori
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=histidine-rich glycoprotein
kn-keyword=histidine-rich glycoprotein
en-keyword=vascular biology
kn-keyword=vascular biology
en-keyword=coagulation
kn-keyword=coagulation
en-keyword=angiogenesis
kn-keyword=angiogenesis
END

start-ver=1.4
cd-journal=joma
no-vol=133
cd-vols=
no-issue=2
article-no=
start-page=96
end-page=98
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The 2020 Incentive Award of the Okayama Medical Association in General Medical Science (2020 Yuuki Prize)
kn-title=令和２年度岡山医学会賞 総合研究奨励賞（結城賞）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=TakahashiYohei
en-aut-sei=Takahashi
en-aut-mei=Yohei
kn-aut-name=��橋陽平
kn-aut-sei=��橋
kn-aut-mei=陽平
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=岡山大学大学院医歯薬学総合研究科　薬理学
END

start-ver=1.4
cd-journal=joma
no-vol=139
cd-vols=
no-issue=
article-no=
start-page=111633
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202107
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Osteopontin silencing attenuates bleomycin-induced murine pulmonary fibrosis by regulating epithelial-mesenchymal transition
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Idiopathic pulmonary fibrosis (IPF) is the most common and most deadly form of interstitial lung disease. Osteopontin (OPN), a matricellular protein with proinflammatory and profibrotic properties, plays a major role in several fibrotic diseases, including IPF; OPN is highly upregulated in patients' lung samples. In this study, we knocked down OPN in a bleomycin (BLM)-induced pulmonary fibrosis (PF) mouse model using small interfering RNA (siRNA) to determine whether the use of OPN siRNA is an effective therapeutic strategy for IPF. We found that fibrosing areas were significantly smaller in specimens from OPN siRNA-treated mice. The number of alveolar macrophages, neutrophils, and lymphocytes in bronchoalveolar lavage fluid was also reduced in OPN siRNA-treated mice. Regarding the expression of epithelial-mesenchymal transition (EMT)-related proteins, the administration of OPN-siRNA to BLM-treated mice upregulated E-cadherin expression and downregulated vimentin expression. Moreover, in vitro, we incubated the human alveolar adenocarcinoma cell line A549 with transforming growth factor (TGF)-beta 1 and subsequently transfected the cells with OPN siRNA. We found a significant upregulation of Col1A1, fibronectin, and vimentin after TGF-beta 1 stimulation in A549 cells. In contrast, a downregulation of Col1A1, fibronectin, and vimentin mRNA levels was observed in TGF-beta 1-stimulated OPN knockdown A549 cells. Therefore, the downregulation of OPN effectively reduced pulmonary fibrotic and EMT changes both in vitro and in vivo. Altogether, our results indicate that OPN siRNA exerts a protective effect on BLM-induced PF in mice. Our results provide a basis for the development of novel targeted therapeutic strategies for IPF.
en-copyright=
kn-copyright=

en-aut-name=HatipogluOmer Faruk
en-aut-sei=Hatipoglu
en-aut-mei=Omer Faruk
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UctepeEyyup
en-aut-sei=Uctepe
en-aut-mei=Eyyup
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OpokuGabriel
en-aut-sei=Opoku
en-aut-mei=Gabriel
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WakeHidenori
en-aut-sei=Wake
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=IkemuraKentaro
en-aut-sei=Ikemura
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OhtsukiTakashi
en-aut-sei=Ohtsuki
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=InagakiJunko
en-aut-sei=Inagaki
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=GunduzMehmet
en-aut-sei=Gunduz
en-aut-mei=Mehmet
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=GunduzEsra
en-aut-sei=Gunduz
en-aut-mei=Esra
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=WatanabeShogo
en-aut-sei=Watanabe
en-aut-mei=Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NishinakaTakashi
en-aut-sei=Nishinaka
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=TakahashiHideo
en-aut-sei=Takahashi
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=HirohataSatoshi
en-aut-sei=Hirohata
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=Department of Pharmacology, Faculty of Medicine, Kindai University
kn-affil=

affil-num=2
en-affil=Ac?badem Labmed Ankara Tissue Typing Laboratory
kn-affil=

affil-num=3
en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Pharmacology, Faculty of Medicine, Kindai University
kn-affil=

affil-num=5
en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Department of Otolaryngology, Moriya Keiyu Hospital
kn-affil=

affil-num=9
en-affil=Department of Otolaryngology, Moriya Keiyu Hospital
kn-affil=

affil-num=10
en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=11
en-affil=Department of Pharmacology, Faculty of Medicine, Kindai University
kn-affil=

affil-num=12
en-affil=Department of Pharmacology, Faculty of Medicine, Kindai University
kn-affil=

affil-num=13
en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University
kn-affil=
en-keyword=Pulmonary fibrosis
kn-keyword=Pulmonary fibrosis
en-keyword=Osteopontin
kn-keyword=Osteopontin
en-keyword=Epithelial-mesenchymal transition
kn-keyword=Epithelial-mesenchymal transition
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=3
article-no=
start-page=357
end-page=362
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202106
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Optimizing the timing of 3.6 mg Pegfilgrastim Administration for Dose-Dense Chemotherapy in Japanese Patients with Breast Cancer
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Perioperative dose-dense chemotherapy (DDCT) with pegfilgrastim (Peg) prophylaxis is a standard treatment for high-risk breast cancer. We explored the optimal timing of administration of 3.6 mg Peg, the dose approved in Japan. In the phase II feasibility study of DDCT (adriamycin+cyclophosphamide or epirubicin+cyclophosphamide followed by paclitaxel) for breast cancer, we investigated the feasibility, safety, neutrophil transition, and optimal timing of Peg treatment by administering Peg at days 2, 3, and 4 post-chemotherapy (P2, P3, and P4 groups, respectively). Among the 52 women enrolled, 13 were aged > 60 years. The anthracycline sequence was administered to P2 (n=33), P3 (n=5), and P4 (n=14) patients, and the taxane sequence to P2 (n=38) and P3 (n=6) patients. Both sequences showed no interaction between Peg administration timing and treatment discontinuation, treatment delay, or dose reduction. However, the relative dose intensity (RDI) was significantly different among the groups. The neutrophil count transition differed significantly among the groups receiving the anthracycline sequence. However, the neutrophil count remained in the appropriate range for both sequences in the P2 group. The timing of Peg administration did not substantially affect the feasibility or safety of DDCT. Postoperative day 2 might be the optimal timing for DDCT.
en-copyright=
kn-copyright=

en-aut-name=TakabatakeDaisuke
en-aut-sei=Takabatake
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KajiwaraYukiko
en-aut-sei=Kajiwara
en-aut-mei=Yukiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OhtaniShoichiro
en-aut-sei=Ohtani
en-aut-mei=Shoichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SuzukiYoko
en-aut-sei=Suzuki
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YamamotoMari
en-aut-sei=Yamamoto
en-aut-mei=Mari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KuboShinichiro
en-aut-sei=Kubo
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=IkedaMasahiko
en-aut-sei=Ikeda
en-aut-mei=Masahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TakahashiMina
en-aut-sei=Takahashi
en-aut-mei=Mina
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=HaraFumikata
en-aut-sei=Hara
en-aut-mei=Fumikata
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=AogiKenjiro
en-aut-sei=Aogi
en-aut-mei=Kenjiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=OhsumiShozo
en-aut-sei=Ohsumi
en-aut-mei=Shozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=OgasawaraYutaka
en-aut-sei=Ogasawara
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=NishiyamaYoshitaka
en-aut-sei=Nishiyama
en-aut-mei=Yoshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=HikinoHajime
en-aut-sei=Hikino
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=MatsuokaKinya
en-aut-sei=Matsuoka
en-aut-mei=Kinya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=ShienTadahiko
en-aut-sei=Shien
en-aut-mei=Tadahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=TairaNaruto
en-aut-sei=Taira
en-aut-mei=Naruto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=DoiharaHiroyoshi
en-aut-sei=Doihara
en-aut-mei=Hiroyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

affil-num=1
en-affil=Kochi Health Science Center
kn-affil=

affil-num=2
en-affil=Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Hiroshima Citizens Hospital
kn-affil=

affil-num=4
en-affil=Okayama University Hospital
kn-affil=

affil-num=5
en-affil=Fukuyama Citizens Hospital
kn-affil=

affil-num=6
en-affil=Fukuyama Citizens Hospital
kn-affil=

affil-num=7
en-affil=Fukuyama Citizens Hospital
kn-affil=

affil-num=8
en-affil=Shikoku Cancer Center
kn-affil=

affil-num=9
en-affil=Cancer Institute Hospital
kn-affil=

affil-num=10
en-affil=Shikoku Cancer Center
kn-affil=

affil-num=11
en-affil=Shikoku Cancer Center
kn-affil=

affil-num=12
en-affil=Kagawa Prefectural Center Hospital
kn-affil=

affil-num=13
en-affil=Okayama Saiseikai General Hospital
kn-affil=

affil-num=14
en-affil=Matsue Red Cross General Hospital
kn-affil=

affil-num=15
en-affil=Ehime Prefectural Central Hospital
kn-affil=

affil-num=16
en-affil=Okayama University Hospital
kn-affil=

affil-num=17
en-affil=Okayama University Hospital
kn-affil=

affil-num=18
en-affil=Okayama University Hospital
kn-affil=
en-keyword=dose-dense chemotherapy
kn-keyword=dose-dense chemotherapy
en-keyword=breast cancer
kn-keyword=breast cancer
en-keyword=pegfilgrastim
kn-keyword=pegfilgrastim
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=3
article-no=
start-page=335
end-page=343
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202106
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Baseline Neutrophil-to-Lymphocyte Ratio and Glasgow Prognostic Score are Associated with Clinical Outcome in Patients with Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma Treated with Nivolumab
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Recurrent or metastatic head and neck squamous cell carcinoma (R/MHNSCC) has a poor prognosis. Although nivolumab is approved in Japan for treating R/MHNSCC, the response rate is low. Therefore, identifying pretreatment prognostic factors is necessary. This study assessed the utility of the neutrophil-to-lymphocyte ratio (NLR) and Glasgow Prognostic Score (GPS) as biomarkers of response to nivolumab. We retrospectively collected the data of 56 R/MHNSCC patients treated with nivolumab between May 2017 and December 2019. The Kaplan?Meier method and log-rank test were used to estimate overall survival (OS) and progression-free survival (PFS), and multivariate Cox hazard regression analysis was used to identify independent predictors of survival. Patients with a low pretreatment NLR had prolonged OS, and patients with a low pretreatment GPS had increased OS and PFS. A performance score (PS) of 0-1, development of immune-related adverse events, and GPS of 0-1 were significantly associated with OS in multivariate analysis. In summary, baseline pretreatment NLR and GPS are independently associated with OS in R/MHNSCC patients treated with nivolumab. Administration of nivolumab while maintaining the PS reflects a immune status of the host and leads to a good OS.
en-copyright=
kn-copyright=

en-aut-name=ChikuieNobuyuki
en-aut-sei=Chikuie
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HamamotoTakao
en-aut-sei=Hamamoto
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UedaTsutomu
en-aut-sei=Ueda
en-aut-mei=Tsutomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TaruyaTakayuki
en-aut-sei=Taruya
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KonoTakashi
en-aut-sei=Kono
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=FuruieHiromi
en-aut-sei=Furuie
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=IshinoTakashi
en-aut-sei=Ishino
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TakenoSachio
en-aut-sei=Takeno
en-aut-mei=Sachio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
kn-affil=

affil-num=2
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
kn-affil=

affil-num=3
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
kn-affil=

affil-num=4
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
kn-affil=

affil-num=5
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
kn-affil=

affil-num=6
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Kure Medical Center and Chugoku Cancer Center
kn-affil=

affil-num=7
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
kn-affil=

affil-num=8
en-affil=Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University
kn-affil=
en-keyword=neutrophil-to-lymphocyte ratio
kn-keyword=neutrophil-to-lymphocyte ratio
en-keyword=nivolumab
kn-keyword=nivolumab
en-keyword=Glasgow Prognostic Score
kn-keyword=Glasgow Prognostic Score
en-keyword=recurrent or metastatic head and neck squamous cell carcinoma (R/MHNSCC)
kn-keyword=recurrent or metastatic head and neck squamous cell carcinoma (R/MHNSCC)
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=好中球細胞外トラップを標的にしたトロンボモジュリンは膵癌転移を予防する
kn-title=Targeting neutrophil extracellular traps with thrombomodulin prevents pancreatic cancer metastasis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=KajiokaHiroki
en-aut-sei=Kajioka
en-aut-mei=Hiroki
kn-aut-name=梶岡裕紀
kn-aut-sei=梶岡
kn-aut-mei=裕紀
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ヒスチジンリッチグリコプロテインはCLEC1A受容体を通じてヒト好中球の貪食を活性化し、生存時間を延長させる
kn-title=Histidine-rich glycoprotein stimulates human neutrophil phagocytosis and prolongs survival through CLEC1A
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=TakahashiYohei
en-aut-sei=Takahashi
en-aut-mei=Yohei
kn-aut-name=��橋陽平
kn-aut-sei=��橋
kn-aut-mei=陽平
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=133
cd-vols=
no-issue=1
article-no=
start-page=10
end-page=22
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Novel understanding of septic pathophysiology and neutrophil extracellular traps
kn-title=敗血症病態の新しい理解と好中球細胞外トラップ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=NishiboriMasahiro
en-aut-sei=Nishibori
en-aut-mei=Masahiro
kn-aut-name=西堀正洋
kn-aut-sei=西堀
kn-aut-mei=正洋
aut-affil-num=1
ORCID=

en-aut-name=WakeHidenori
en-aut-sei=Wake
en-aut-mei=Hidenori
kn-aut-name=和氣秀徳
kn-aut-sei=和氣
kn-aut-mei=秀徳
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=岡山大学大学院医歯薬学総合研究科　薬理学

affil-num=2
en-affil=Department of Pharmacology, Faculty of Medicine, Kindai University
kn-affil=近畿大学医学部　薬理学
en-keyword=敗血症
kn-keyword=敗血症
en-keyword=histidine-rich glycoprotein（HRG）
kn-keyword=histidine-rich glycoprotein（HRG）
en-keyword=好中球
kn-keyword=好中球
en-keyword=NETs
kn-keyword=NETs
en-keyword=血管内皮細胞
kn-keyword=血管内皮細胞
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=1
article-no=
start-page=150
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Loss of IL-33 enhances elastase-induced and cigarette smoke extract-induced emphysema in mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background IL-33, which is known to induce type 2 immune responses via group 2 innate lymphoid cells, has been reported to contribute to neutrophilic airway inflammation in chronic obstructive pulmonary disease. However, its role in the pathogenesis of emphysema remains unclear. Methods We determined the role of interleukin (IL)-33 in the development of emphysema using porcine pancreas elastase (PPE) and cigarette smoke extract (CSE) in mice. First, IL-33(-/-) mice and wild-type (WT) mice were given PPE intratracheally. The numbers of inflammatory cells, and the levels of cytokines and chemokines in the bronchoalveolar lavage (BAL) fluid and lung homogenates, were analyzed; quantitative morphometry of lung sections was also performed. Second, mice received CSE by intratracheal instillation. Quantitative morphometry of lung sections was then performed again. Results Intratracheal instillation of PPE induced emphysematous changes and increased IL-33 levels in the lungs. Compared to WT mice, IL-33(-/-) mice showed significantly greater PPE-induced emphysematous changes. No differences were observed between IL-33(-/-) and WT mice in the numbers of macrophages or neutrophils in BAL fluid. The levels of hepatocyte growth factor were lower in the BAL fluid of PPE-treated IL-33(-/-) mice than WT mice. IL-33(-/-) mice also showed significantly greater emphysematous changes in the lungs, compared to WT mice, following intratracheal instillation of CSE. Conclusion These observations suggest that loss of IL-33 promotes the development of emphysema and may be potentially harmful to patients with COPD.
en-copyright=
kn-copyright=

en-aut-name=MorichikaDaisuke
en-aut-sei=Morichika
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TaniguchiAkihiko
en-aut-sei=Taniguchi
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OdaNaohiro
en-aut-sei=Oda
en-aut-mei=Naohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FujiiUtako
en-aut-sei=Fujii
en-aut-mei=Utako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SenooSatoru
en-aut-sei=Senoo
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ItanoJunko
en-aut-sei=Itano
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KanehiroArihiko
en-aut-sei=Kanehiro
en-aut-mei=Arihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KitaguchiYoshiaki
en-aut-sei=Kitaguchi
en-aut-mei=Yoshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=YasuoMasanori
en-aut-sei=Yasuo
en-aut-mei=Masanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HanaokaMasayuki
en-aut-sei=Hanaoka
en-aut-mei=Masayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SatohTakashi
en-aut-sei=Satoh
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=AkiraShizuo
en-aut-sei=Akira
en-aut-mei=Shizuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KiuraKatsuyuki
en-aut-sei=Kiura
en-aut-mei=Katsuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=MaedaYoshinobu
en-aut-sei=Maeda
en-aut-mei=Yoshinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=MiyaharaNobuaki
en-aut-sei=Miyahara
en-aut-mei=Nobuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

affil-num=1
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=First Department of Internal Medicine, Shinshu University School of Medicine
kn-affil=

affil-num=9
en-affil=First Department of Internal Medicine, Shinshu University School of Medicine
kn-affil=

affil-num=10
en-affil=First Department of Internal Medicine, Shinshu University School of Medicine
kn-affil=

affil-num=11
en-affil=Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Center (WPI?IFReC), Osaka University
kn-affil=

affil-num=12
en-affil=Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Center (WPI?IFReC), Osaka University
kn-affil=

affil-num=13
en-affil=Department of Allergy and Respiratory Medicine, Okayama University
kn-affil=

affil-num=14
en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of Allergy and Respiratory Medicine, Okayama University
kn-affil=
en-keyword=Chronic obstructive pulmonary disease
kn-keyword=Chronic obstructive pulmonary disease
en-keyword=COPD
kn-keyword=COPD
en-keyword=HGF
kn-keyword=HGF
en-keyword=VEGF
kn-keyword=VEGF
END

start-ver=1.4
cd-journal=joma
no-vol=132
cd-vols=
no-issue=3
article-no=
start-page=117
end-page=119
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The 2019 Incentive Award of the Okayama Medical Association in General Medical Science (2019 Yuuki Prize)
kn-title=令和元年度岡山医学会賞 総合研究奨励賞（結城賞）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Soe Soe　Htwe
en-aut-sei=Soe Soe　Htwe
en-aut-mei=
kn-aut-name=ソウ　ソウ トウエ
kn-aut-sei=ソウ　ソウ トウエ
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Pharmacology, University of medicine 2, Yangon
kn-affil=ヤンゴン第二医科大学医学部　薬理学
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=2
article-no=
start-page=153
end-page=167
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202104
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Lactoferrin-like Immunoreactivity in Distinct Neuronal Populations in the Mouse Central Nervous System
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Lactoferrin (Lf) is an iron-binding glycoprotein mainly found in exocrine secretions and the secondary granules of neutrophils. In the central nervous system (CNS), expression of the Lf protein has been reported in the lesions of some neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis, as well as in the aged brain. Lf is primarily considered an iron chelator, protecting cells from potentially toxic iron or iron-requiring microorganisms. Other biological functions of Lf include immunomodulation and transcriptional regulation. However, the roles of Lf in the CNS have yet to be fully clarified. In this study, we raised an antiserum against mouse Lf and investigated the immunohistochemical localization of Lf-like immunoreactivity (Lf-LI) throughout the CNS of adult mice. Lf-LI was found in some neuronal populations throughout the CNS. Intense labeling was found in neurons in the olfactory systems, hypothalamic nuclei, entorhinal cortex, and a variety of brainstem nuclei. This study provides detailed information on the Lf-LI distribution in the CNS, and the findings should promote further understanding of both the physiological and pathological significance of Lf in the CNS.
en-copyright=
kn-copyright=

en-aut-name=ShimaokaShigeyoshi
en-aut-sei=Shimaoka
en-aut-mei=Shigeyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HamaokaHitomi
en-aut-sei=Hamaoka
en-aut-mei=Hitomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=InoueJunji
en-aut-sei=Inoue
en-aut-mei=Junji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AsanumaMasato
en-aut-sei=Asanuma
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TooyamaIkuo
en-aut-sei=Tooyama
en-aut-mei=Ikuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KondoYoichi
en-aut-sei=Kondo
en-aut-mei=Yoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=2
en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=3
en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=4
en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Molecular Neuroscience Research Center, Shiga University of Medical Science
kn-affil=

affil-num=6
en-affil=Molecular Neuroscience Research Center, Shiga University of Medical Science
kn-affil=
en-keyword=lactoferrin
kn-keyword=lactoferrin
en-keyword=immunohistochemistry
kn-keyword=immunohistochemistry
en-keyword=brain mapping
kn-keyword=brain mapping
END

start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=
article-no=
start-page=70
end-page=77
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Prospective cohort study of febrile neutropenia in breast cancer patients administered with neoadjuvant and adjuvant chemotherapies: CSPOR-BC FN study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background<br/>
As Asians are more vulnerable to febrile neutropenia (FN) than Caucasians, evaluations of FN incidence and risk factors in Asians are important for the appropriate use of primary pegfilgrastim (PEG-G). <br/>
Patients and methods<br/>
Japanese breast cancer patients receiving standard adjuvant chemotherapies were prospectively enrolled in multicenter institutions from August 2015 to July 2017. FN was evaluated from 2 treatment policies: true FN (T-FN): ?37.5?°C, grade 4 neutropenia, mandatory hospital visit (visiting); surrogate FN (S-FN): ?37.5?°C, oral antibiotic, no mandatory visit (non-visiting). PEG-G was used at the physicians’ discretion. The primary endpoint was FN incidence during all cycles. Multivariate logistic regression analysis was performed to identify T-FN risk factors. <br/>
Results<br/>
Of 1005 enrolled patients, 980 women treated with FEC, E(A)C, and TC were analyzed. The FN incidence proportions in all patients were 22.5%, 27.5%, and 33.9% for FEC, E(A)C, and TC, respectively. Those of T-FN were 27.7%, 22.4%, and 36.6%; those of S-FN were 17.3%, 32.4%, and 31.5% with more frequent primary PEG-G usage. The relative dose intensity (RDI) of the 3 regimens was ?0.85 in both groups. In the analysis of risk factors, TC (odds ratio?=?2.67), age???65 years (2.24), and pretreatment absolute neutrophil count (ANC)/1000?μl (0.8) remained significant. <br/>
Conclusions<br/>
FN incidences were above 20% in the 3 regimens, with TC showing the highest. RDI was maintained at a high level in both visiting and non-visiting groups. Patient-related risk factors were age and pretreatment ANC.
en-copyright=
kn-copyright=

en-aut-name=IshikawaTakashi
en-aut-sei=Ishikawa
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SakamakiKentaro
en-aut-sei=Sakamaki
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NaruiKazutaka
en-aut-sei=Narui
en-aut-mei=Kazutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NishimuraHideki
en-aut-sei=Nishimura
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SangaiTakafumi
en-aut-sei=Sangai
en-aut-mei=Takafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TamakiKentaro
en-aut-sei=Tamaki
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HasegawaYoshie
en-aut-sei=Hasegawa
en-aut-mei=Yoshie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=WatanabeKen-ichi
en-aut-sei=Watanabe
en-aut-mei=Ken-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SuganumaNobuyasu
en-aut-sei=Suganuma
en-aut-mei=Nobuyasu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MichishitaShintaro
en-aut-sei=Michishita
en-aut-mei=Shintaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SugaeSadatoshi
en-aut-sei=Sugae
en-aut-mei=Sadatoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=AiharaTomohiko
en-aut-sei=Aihara
en-aut-mei=Tomohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=TsugawaKoichiro
en-aut-sei=Tsugawa
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=KaiseHirose
en-aut-sei=Kaise
en-aut-mei=Hirose
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=TairaNaruto
en-aut-sei=Taira
en-aut-mei=Naruto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=MukaiHirofumi
en-aut-sei=Mukai
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

affil-num=1
en-affil=Department of Breast Surgery and Oncology, Tokyo Medical University
kn-affil=

affil-num=2
en-affil=Department of Biostatistics, Yokohama City University
kn-affil=

affil-num=3
en-affil=Department of Biostatistics, Yokohama City University
kn-affil=

affil-num=4
en-affil=Department of Biostatistics, Yokohama City University
kn-affil=

affil-num=5
en-affil=Department of Breast and Thyroid Surgery, Chiba University
kn-affil=

affil-num=6
en-affil=Naha-Nishi Clinic
kn-affil=

affil-num=7
en-affil=Department of Breast Surgery, Hirosaki Municipal Hospita
kn-affil=

affil-num=8
en-affil=Department of Breast Surgery, Hokkaido Cancer Center
kn-affil=

affil-num=9
en-affil=Department of Breast and Thyroid Surgery, Kanagawa Cancer Center
kn-affil=

affil-num=10
en-affil=Department of Breast Surgery, Yao Municipal Hospital
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine
kn-affil=

affil-num=12
en-affil=Breast Center, Aihara Hospital
kn-affil=

affil-num=13
en-affil=Department of Breast and Thyroid Surgery, St. Marianna University
kn-affil=

affil-num=14
en-affil=Department of Breast Surgery and Oncology, Tokyo Medical University
kn-affil=

affil-num=15
en-affil=Department of Breast and Endocrinology Surgery, Okayama University Graduate School of Medicine
kn-affil=

affil-num=16
en-affil=Division of Oncology/Hematology, National Cancer Center Hospital East
kn-affil=
en-keyword=Breast cancer
kn-keyword=Breast cancer
en-keyword=Febrile neutropenia
kn-keyword=Febrile neutropenia
en-keyword=Adjuvant chemotherapy
kn-keyword=Adjuvant chemotherapy
en-keyword=Risk factors
kn-keyword=Risk factors
en-keyword=Prospective study
kn-keyword=Prospective study
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201227
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=分化誘導型好中球様細胞株におけるHistidine-Rich Glycoprotein (HRG)の効果
kn-title=An evaluation of the activity of histidine-rich glycoprotein on differentiated neutrophil-like cells from human cell lines
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=YoshiiYukinori
en-aut-sei=Yoshii
en-aut-mei=Yukinori
kn-aut-name=吉井將哲
kn-aut-sei=吉井
kn-aut-mei=將哲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=497
cd-vols=
no-issue=
article-no=
start-page=1
end-page=3
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210128
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Targeting neutrophil extracellular traps with thrombomodulin prevents pancreatic cancer metastasis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Surgery is the only curative treatment option for pancreatic cancer, but patients often develop postoperative recurrence. Surgical invasiveness might be involved in the mechanism of recurrence. The associations among inflammation caused by surgery, neutrophils, and cancer metastasis were investigated. At first, neutrophil extracellular traps (NETs) were examined in clinical specimens, and NETs were observed around metastatic tumors. To explore how NETs were induced, neutrophils were cultured with pancreatic cancer or in cancer-conditioned medium. Neutrophils formed NETs when they were cultured with pancreatic cancer or even its conditioned medium. The effects of NETs on cancer cells were further investigated in vitro and in vivo. NETs induced the epithelial to mesenchymal transition in cancer cells and thereby promoted their migration and invasion. HMGB1 derived from NETs appeared to potentiate the malignancy of cancer cells. In a mouse model of liver metastasis with inflammation, NETs participated in the metastatic process by enhancing extravasation. Interestingly, thrombomodulin degraded HMGB1 and consequently inhibited the induction of NETs, thereby preventing pancreatic cancer metastasis to the liver. In conclusion, NETs interact reciprocally with pancreatic cancer cells, which play a pivotal role in inflammation-associated metastasis. Targeting NETs with thrombomodulin can be a novel strategy to improve the surgical outcome of pancreatic cancer patients.
en-copyright=
kn-copyright=

en-aut-name=KajiokaHiroki
en-aut-sei=Kajioka
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KagawaShunsuke
en-aut-sei=Kagawa
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ItoAtene
en-aut-sei=Ito
en-aut-mei=Atene
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YoshimotoMasashi
en-aut-sei=Yoshimoto
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SakamotoShuichi
en-aut-sei=Sakamoto
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KikuchiSatoru
en-aut-sei=Kikuchi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KurodaShinji
en-aut-sei=Kuroda
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YoshidaRyuichi
en-aut-sei=Yoshida
en-aut-mei=Ryuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=UmedaYuzo
en-aut-sei=Umeda
en-aut-mei=Yuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=NomaKazuhiro
en-aut-sei=Noma
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=TazawaHiroshi
en-aut-sei=Tazawa
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

affil-num=1
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=HMGB1
kn-keyword=HMGB1
en-keyword=Epithelial to mesenchymal transition
kn-keyword=Epithelial to mesenchymal transition
en-keyword=Phorbol 12-myristate 13-acetate
kn-keyword=Phorbol 12-myristate 13-acetate
en-keyword=Ischemia-reperfusion model
kn-keyword=Ischemia-reperfusion model
END

start-ver=1.4
cd-journal=joma
no-vol=134
cd-vols=
no-issue=20
article-no=
start-page=2771
end-page=2787
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Inhibition of interleukin-6 signaling attenuates aortitis, left ventricular hypertrophy and arthritis in interleukin-1 receptor antagonist deficient mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The aim of the present study was to examine whether inhibition of Interleukin (IL)-6 signaling by MR16-1, an IL-6 receptor antibody, attenuates aortitis, cardiac hypertrophy, and arthritis in IL-1 receptor antagonist deficient (IL-1RA KO) mice. Four weeks old mice were intraperitoneally administered with either MR16-1 or non-immune IgG at dosages that were adjusted over time for 5 weeks. These mice were stratified into four groups: MR16-1 treatment groups, KO/MR low group (first 2.0 mg, following 0.5 mg/week, n=14) and KO/MR high group (first 4.0 mg, following 2.0 mg/week, n=19) in IL-1RA KO mice, and IgG treatment groups, KO/IgG group (first 2.0 mg, following 1.0 mg/week, n=22) in IL-1RA KO mice, and wild/IgG group (first 2.0 mg, following 1.0 mg/week, n=17) in wild mice. Aortitis, cardiac hypertrophy and arthropathy were histologically analyzed. Sixty-eight percent of the KO/IgG group developed aortitis (53% developed severe aortitis). In contrast, only 21% of the KO/MR high group developed mild aortitis, without severe aortitis (P<0.01, vs KO/IgG group). Infiltration of inflammatory cells, such as neutrophils, T cells, and macrophages, was frequently observed around aortic sinus of the KO/IgG group. Left ventricle and cardiomyocyte hypertrophy were observed in IL-1RA KO mice. Administration of high dosage of MR16-1 significantly suppressed cardiomyocyte hypertrophy. MR16-1 attenuated the incidence and severity of arthritis in IL-1RA KO mice in a dose-dependent manner. In conclusion, blockade of IL-6 signaling may exert a beneficial effect to attenuate severe aortitis, left ventricle hypertrophy, and arthritis.
en-copyright=
kn-copyright=

en-aut-name=HadaYoshiko
en-aut-sei=Hada
en-aut-mei=Yoshiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UchidaHaruhito A.
en-aut-sei=Uchida
en-aut-mei=Haruhito A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MukaiTomoyuki
en-aut-sei=Mukai
en-aut-mei=Tomoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KojimaFumiaki
en-aut-sei=Kojima
en-aut-mei=Fumiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshidaMasashi
en-aut-sei=Yoshida
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakeuchiHidemi
en-aut-sei=Takeuchi
en-aut-mei=Hidemi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KakioYuki
en-aut-sei=Kakio
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OtakaNozomu
en-aut-sei=Otaka
en-aut-mei=Nozomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=MoritaYoshitaka
en-aut-sei=Morita
en-aut-mei=Yoshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
kn-affil=

affil-num=3
en-affil=Department of Rheumatology, Kawasaki Medical School
kn-affil=

affil-num=4
en-affil=Department of Pharmacology, Kitasato University School of Allied Health Sciences
kn-affil=

affil-num=5
en-affil=Department of Chronic Kidney Disease and Cardiovascular Disease, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
kn-affil=

affil-num=6
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
kn-affil=

affil-num=7
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
kn-affil=

affil-num=8
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
kn-affil=

affil-num=9
en-affil=Department of Rheumatology, Kawasaki Medical School
kn-affil=

affil-num=10
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
kn-affil=
en-keyword= MR16-1
kn-keyword= MR16-1
en-keyword=interleukin-1 receptor antagonist
kn-keyword=interleukin-1 receptor antagonist
en-keyword=aortitis
kn-keyword=aortitis
en-keyword=arthritis
kn-keyword=arthritis
en-keyword=LV hypertrophy
kn-keyword=LV hypertrophy
END

start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=1
article-no=
start-page=6869
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200422
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Deficiency of CD44 prevents thoracic aortic dissection in a murine model
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Thoracic aortic dissection (TAD) is a life-threatening vascular disease. We showed that CD44, a widely distributed cell surface adhesion molecule, has an important role in inflammation. In this study, we examined the role of CD44 in the development of TAD. TAD was induced by the continuous infusion of beta-aminopropionitrile (BAPN), a lysyl oxidase inhibitor, and angiotensin II (AngII) for 7 days in wild type (WT) mice and CD44 deficient (CD44(-/-)) mice. The incidence of TAD in CD44(-/-) mice was significantly reduced compared with WT mice (44% and 6%, p<0.01). Next, to evaluate the initial changes, aortic tissues at 24hours after BAPN/AngII infusion were examined. Neutrophil accumulation into thoracic aortic adventitia in CD44(-/-) mice was significantly decreased compared with that in WT mice (5.7 +/- 0.3% and 1.6 +/- 0.4%, p<0.01). In addition, BAPN/AngII induced interleukin-6, interleukin-1 beta, matrix metalloproteinase-2 and matrix metalloproteinase-9 in WT mice, all of which were significantly reduced in CD44(-/-) mice (all p<0.01). In vitro transmigration of neutrophils from CD44(-/-) mice through an endothelial monolayer was significantly decreased by 18% compared with WT mice (p<0.01). Our findings indicate that CD44 has a critical role in TAD development in association with neutrophil infiltration into adventitia.
en-copyright=
kn-copyright=

en-aut-name=HatipogluOmer F.
en-aut-sei=Hatipoglu
en-aut-mei=Omer F.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YonezawaTomoko
en-aut-sei=Yonezawa
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KondoMegumi
en-aut-sei=Kondo
en-aut-mei=Megumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=AmiokaNaofumi
en-aut-sei=Amioka
en-aut-mei=Naofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YoshidaMasashi
en-aut-sei=Yoshida
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=AkagiSatoshi
en-aut-sei=Akagi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=HirohataSatoshi
en-aut-sei=Hirohata
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=3
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=4
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=5
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=6
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=7
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=8
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=

affil-num=9
en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=10
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
kn-affil=
en-keyword=Aneurysm
kn-keyword=Aneurysm
en-keyword=Aortic diseases
kn-keyword=Aortic diseases
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200803
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The neutrophil-to-lymphocyte ratio as a novel independent prognostic factor for multiple metastatic lung tumors from various sarcomas
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Purpose</br>
Sarcomas are among the most refractory malignant tumors and often recur as pulmonary metastasis. Although the presence of a high neutrophil-to-lymphocyte ratio (NLR) has been associated with the prognosis of several malignancies, the relationship between the NLR and sarcoma with pulmonary metastasis is unclear. We investigated the impact of the NLR in patients who underwent surgical resection for metastatic lung tumors from various sarcomas.</br>
Methods</br>
The subjects of this retrospective study were 158 patients with metastatic lung tumors from various sarcomas, who underwent initial pulmonary metastasectomy between 2006 and 2015. We examined the clinicopathological variables, including the NLR and the characteristics of surgical procedures. Survival was estimated by the Kaplan?Meier method and prognostic factors were evaluated by multivariate analysis.</br>
Results</br>
Multivariate analysis revealed significantly better survival of the group with an NLR?<?2.26 immediately before the most recent pulmonary metastasectomy, in addition to such factors as the largest resected lesion being?<?22 mm, a disease-free interval of?>?2 years, and 3 or more pulmonary metastasectomies.</br>
Conclusion</br>
The NLR immediately before the most recent pulmonary metastasectomy is a novel independent prognostic factor, which may be helpful when considering repeated pulmonary metastasectomy.
en-copyright=
kn-copyright=

en-aut-name=YamamotoHiromasa
en-aut-sei=Yamamoto
en-aut-mei=Hiromasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NambaKei
en-aut-sei=Namba
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamamotoHaruchika
en-aut-sei=Yamamoto
en-aut-mei=Haruchika
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TojiTomohiro
en-aut-sei=Toji
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SohJunichi
en-aut-sei=Soh
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ShienKazuhiko
en-aut-sei=Shien
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SuzawaKen
en-aut-sei=Suzawa
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KurosakiTakeshi
en-aut-sei=Kurosaki
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OtaniShinji
en-aut-sei=Otani
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=OkazakiMikio
en-aut-sei=Okazaki
en-aut-mei=Mikio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SugimotoSeiichiro
en-aut-sei=Sugimoto
en-aut-mei=Seiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=YamaneMasaomi
en-aut-sei=Yamane
en-aut-mei=Masaomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=TakahashiKatsuhito
en-aut-sei=Takahashi
en-aut-mei=Katsuhito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=KunisadaToshiyuki
en-aut-sei=Kunisada
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=OtoTakahiro
en-aut-sei=Oto
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=ToyookaShinichi
en-aut-sei=Toyooka
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

affil-num=1
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=4
en-affil=Department of Diagnostic Pathology, Okayama University Hospital
kn-affil=

affil-num=5
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=6
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=7
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=8
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=9
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=10
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=11
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=12
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=13
en-affil=Center for Multidisciplinary Treatment of Sarcoma, Department of Sarcoma Medicine, Kameda Medical Center
kn-affil=

affil-num=14
en-affil=Department of Orthopedic Surgery, Okayama University Hospital
kn-affil=

affil-num=15
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=

affil-num=16
en-affil=Department of Thoracic Surgery, Okayama University Hospital
kn-affil=
en-keyword=Metastatic lung tumor
kn-keyword=Metastatic lung tumor
en-keyword=Sarcoma
kn-keyword=Sarcoma
en-keyword=Metastasectomy
kn-keyword=Metastasectomy
en-keyword=Survival rate
kn-keyword=Survival rate
en-keyword=Neutrophil-to-lymphocyte ratio (NLR)
kn-keyword=Neutrophil-to-lymphocyte ratio (NLR)
END

start-ver=1.4
cd-journal=joma
no-vol=21
cd-vols=
no-issue=12
article-no=
start-page=4545
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200626
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Genetic Deletion of Vasohibin-2 Exacerbates Ischemia-Reperfusion-Induced Acute Kidney Injury
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Acute kidney injury (AKI) has been increasingly recognized as a risk factor for transition to chronic kidney disease. Recent evidence suggests that endothelial damage in peritubular capillaries can accelerate the progression of renal injury. Vasohibin-2 (VASH2) is a novel proangiogenic factor that promotes tumor angiogenesis. However, the pathophysiological roles of VASH2 in kidney diseases remain unknown. In the present study, we examined the effects of VASH2 deficiency on the progression of ischemia-reperfusion (I/R) injury-induced AKI. I/R injury was induced by bilaterally clamping renal pedicles for 25 min in male wild-type (WT) andVash2homozygous knockout mice. Twenty-four hours later, I/R injury-induced renal dysfunction and tubular damage were more severe in VASH2-deficient mice than in WT mice, with more prominent neutrophil infiltration and peritubular capillary loss. After induction of I/R injury, VASH2 expression was markedly increased in injured renal tubules. These results suggest that VASH2 expression in renal tubular epithelial cells might be essential for alleviating I/R injury-induced AKI, probably through protecting peritubular capillaries and preventing inflammatory infiltration.
en-copyright=
kn-copyright=

en-aut-name=MiyakeHiromasa
en-aut-sei=Miyake
en-aut-mei=Hiromasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TanabeKatsuyuki
en-aut-sei=Tanabe
en-aut-mei=Katsuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanimuraSatoshi
en-aut-sei=Tanimura
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NakashimaYuri
en-aut-sei=Nakashima
en-aut-mei=Yuri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MoriokaTomoyo
en-aut-sei=Morioka
en-aut-mei=Tomoyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MasudaKana
en-aut-sei=Masuda
en-aut-mei=Kana
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SugiyamaHitoshi
en-aut-sei=Sugiyama
en-aut-mei=Hitoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=SatoYasufumi
en-aut-sei=Sato
en-aut-mei=Yasufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Human Resource Development of Dialysis Therapy for Kidney Disease, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=New Industry Creation Hatchery Center, Tohoku University
kn-affil=

affil-num=9
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=acute kidney injury
kn-keyword=acute kidney injury
en-keyword=ischemia-reperfusion
kn-keyword=ischemia-reperfusion
en-keyword=oxidative stress
kn-keyword=oxidative stress
en-keyword=vasohibin-2
kn-keyword=vasohibin-2
en-keyword=peritubular capillaries
kn-keyword=peritubular capillaries
END

start-ver=1.4
cd-journal=joma
no-vol=23
cd-vols=
no-issue=6
article-no=
start-page=101180
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200626
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Histidine-Rich Glycoprotein Inhibits High-Mobility Group Box-1-Mediated Pathways in Vascular Endothelial Cells through CLEC-1A
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=High-mobility group box-1 (HMGB1) protein has been postulated to play a pathogenic role in severe sepsis. Histidine-rich glycoprotein (HRG), a 75 kDa plasma protein, was demonstrated to improve the survival rate of septic mice through the regulation of neutrophils and endothelium barrier function. As the relalionship of HRG and HMGB1 remains poorly understood, we investigated the effects of HRG on HMGB1-mediated pathway in endothelial cells, focusing on the involvement of specific receptors for HRG. HRC potently inhibited the HMGB1 mobilization and effectively suppressed rHMGB1-induced inflammatory responses and expression of all three HMGB1 receptors in endothelial cells. Moreover, we first clarified that these protective effects of HRG on endothelial cells were mediated through C-type lectin domain family 1 member A (CLEC-1A) receptor. Thus, current study elueiates protective effects of HRG on vascular endothelial cells through inhintion of HMGB1-mediated pathways may contribute to the therapeutic effects of HRG on severe sepsis.
en-copyright=
kn-copyright=

en-aut-name=GaoShangze
en-aut-sei=Gao
en-aut-mei=Shangze
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WakeHidenori
en-aut-sei=Wake
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SakaguchiMasakiyo
en-aut-sei=Sakaguchi
en-aut-mei=Masakiyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WangDengli
en-aut-sei=Wang
en-aut-mei=Dengli
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakahashiYouhei
en-aut-sei=Takahashi
en-aut-mei=Youhei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TeshigawaraKiyoshi
en-aut-sei=Teshigawara
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=ZhongHui
en-aut-sei=Zhong
en-aut-mei=Hui
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MoriShuji
en-aut-sei=Mori
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=LiuKeyue
en-aut-sei=Liu
en-aut-mei=Keyue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=TakahashiHideo
en-aut-sei=Takahashi
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NishiboriMasahiro
en-aut-sei=Nishibori
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Cell Biology,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Pharmacology, School of Pharmacy, Shujitsu University
kn-affil=

affil-num=9
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Pharmacology, Faculty of Medicine, Kindai University
kn-affil=

affil-num=11
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=44
cd-vols=
no-issue=9
article-no=
start-page=1919
end-page=1933
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200521
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=G‐CSF‐dependent neutrophil differentiation requires downregulation of MAPK activities through the Gab2 signaling pathway
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Granulocyte colony‐stimulating factor (G‐CSF) stimulation of myeloid cells induced tyrosine‐phosphorylation of cellular proteins. One of the tyrosine‐phosphorylated proteins was found to be a scaffold protein, Grb2‐associated binding protein 2 (Gab2). Another member of Gab family protein, Gab3, was exogenously overexpressed in neutrophil progenitor cells to make the Gab3 protein to compete with the endogenous Gab2 for the G‐CSF‐dependent signaling. In Gab3‐overexpressed cells, the level of tyrosine phosphorylation of endogenous Gab2 by G‐CSF stimulation was markedly downregulated, while the phosphorylation of Gab3 was significantly enhanced. The Gab3‐overexpressed cells continuously proliferated in the medium containing G‐CSF and lost the ability to differentiate to the mature neutrophil, characterized by the lobulated nucleus. The G‐CSF stimulation‐dependent tyrosine phosphorylation of Gab3, the association of SHP2 to Gab3 and the following mitogen‐activated protein kinase (MAPK) activation were prolonged in the Gab3‐overexpressed cells, compared to the parental cells, where the binding of SHP2 to Gab2 protein and thereby the activation of MAPK were not sustained after G‐CSF stimulation. Inhibition of MAPK by pharmaceutical inhibitor restored the Gab3‐overexpressed cells to the ability to differentiate to mature neutrophil. Therefore, G‐CSF‐dependent Gab2 phosphorylation and following its downregulation led the short‐term MAPK activation. The downregulation of MAPK after transient Gab2 phosphorylation was necessary for the consequent neutrophil differentiation induced by G‐CSF stimulation.
en-copyright=
kn-copyright=

en-aut-name=ZhaoXianglin
en-aut-sei=Zhao
en-aut-mei=Xianglin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KawanoShun‐ichiro
en-aut-sei=Kawano
en-aut-mei=Shun‐ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MasudaJunko
en-aut-sei=Masuda
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MurakamiHiroshi
en-aut-sei=Murakami
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil= Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
en-keyword=granulocyte
kn-keyword=granulocyte
en-keyword=MAP kinase
kn-keyword=MAP kinase
en-keyword=negative feedback
kn-keyword=negative feedback
en-keyword=phosphorylation
kn-keyword=phosphorylation
en-keyword=scaffold protein
kn-keyword=scaffold protein
en-keyword=SHP2
kn-keyword=SHP2
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=5
article-no=
start-page=379
end-page=382
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=201910
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Histidine-rich Glycoprotein Modulates the Blood-vascular System in Septic Condition
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Histidine-rich glycoprotein (HRG) is a 75 kDa glycoprotein synthesized in the liver whose plasma concentration is 100-150 μg/ml. HRG has been shown to modulate sepsis-related biological reactions by binding to several substances and cells, including heparin, factor XII, fibrinogen, thrombospondin, plasminogen, C1q, IgG, heme, LPS, dead cells, bacteria, and fungi. Therefore, reduction of plasma HRG levels in sepsis leads to dysregulation of coagulation, fibrinolysis, and immune response, resulting in disseminated intravascular coagulation and multiple organ failure. This review summarizes the binding and functional properties of HRG in sepsis.
en-copyright=
kn-copyright=

en-aut-name=WakeHidenori
en-aut-sei=Wake
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=htidine-rich glycoprotein
kn-keyword=htidine-rich glycoprotein
en-keyword=septic pathogenesis
kn-keyword=septic pathogenesis
en-keyword=immunothrombosis
kn-keyword=immunothrombosis
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=4
article-no=
start-page=315
end-page=323
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=201908
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Construction and Characterization of a PGN_0297 Mutant of Porphyromonas gingivalis: Evidence of the Contribution of PGN_0297 to Gingipain Activity
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism’s virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains.
en-copyright=
kn-copyright=

en-aut-name=OnoShintaro
en-aut-sei=Ono
en-aut-mei=Shintaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NakayamaMasaaki
en-aut-sei=Nakayama
en-aut-mei=Masaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TachibanaMasato
en-aut-sei=Tachibana
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Abu Saleh Muhammad Shahriar
en-aut-sei=Abu Saleh Muhammad Shahriar
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HelingWang
en-aut-sei=Heling
en-aut-mei=Wang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakashibaShogo
en-aut-sei=Takashiba
en-aut-mei=Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OharaNaoya
en-aut-sei=Ohara
en-aut-mei=Naoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=periodontitis
kn-keyword=periodontitis
en-keyword=Porphyromonas gingivalis
kn-keyword=Porphyromonas gingivalis
en-keyword=gingipain
kn-keyword=gingipain
en-keyword=C-terminal domain
kn-keyword=C-terminal domain
en-keyword=secretion system
kn-keyword=secretion system
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=インターアルファインヒビタータンパクは好中球形態を制御し、活性酸素分子種産生を抑制することによって好中球の静穏状態を維持する
kn-title=Inter-alpha inhibitor proteins maintain neutrophils in a resting state by regulating shape and reducing ROS production
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=SOE SOE HTWE
en-aut-sei=SOE SOE HTWE
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=6
article-no=
start-page=591
end-page=593
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=201812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Neutrophil to Lymphocyte Ratio Is Superior to Other Inflammation-Based Prognostic Scores in Predicting the Mortality of Patients with Pneumonia
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= A neutrophil-to-lymphocyte ratio (NLR) > 7 is reportedly an independent marker of mortality in patients with bacteremia. However, no studies have shown an association between inflammation-based prognostic scores (including the Glasgow Prognostic Score, the NLR, the platelet-to-lymphocyte ratio, the Prognostic Nutritional Index, and the Prognostic Index) and mortality in patients with pneumonia. We retrospectively examined the cases of 33 patients diagnosed with pneumonia who were treated in the ICU of Osaka Medical College Hospital between January 2014 and June 2016. A multivariate analysis revealed that the NLR was a significant predictor of mortality in these pneumonia patients.
en-copyright=
kn-copyright=

en-aut-name=ShimoyamaYuichiro
en-aut-sei=Shimoyama
en-aut-mei=Yuichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UmegakiOsamu
en-aut-sei=Umegaki
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Inoue Satsuki
en-aut-sei=Inoue
en-aut-mei= Satsuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AguiTomoyuki
en-aut-sei=Agui
en-aut-mei=Tomoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KadonoNoriko
en-aut-sei=Kadono
en-aut-mei=Noriko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MinamiToshiaki
en-aut-sei=Minami
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Anesthesiology, Osaka Medical College, Intensive Care Unit
kn-affil=

affil-num=2
en-affil=Department of Anesthesiology, Osaka Medical College, Intensive Care Unit
kn-affil=

affil-num=3
en-affil=Department of Surgery, Osaka Medical College, Intensive Care Unit
kn-affil=

affil-num=4
en-affil=Department of Anesthesiology, Osaka Medical College, Osaka Medical College Hospital
kn-affil=

affil-num=5
en-affil=Department of Anesthesiology, Osaka Medical College, Intensive Care Unit
kn-affil=

affil-num=6
en-affil=Department of Surgery, Osaka Medical College, Intensive Care Unit
kn-affil=
en-keyword=inflammation-based prognostic score
kn-keyword=inflammation-based prognostic score
en-keyword=pneumonia
kn-keyword=pneumonia
en-keyword=in-hospital mortality
kn-keyword=in-hospital mortality
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=5
article-no=
start-page=377
end-page=381
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=201610
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Case of Streptobacillus moniliformis Infection with Cutaneous Leukocytoclastic Vasculitis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A 71-year-old man presented with a high fever, polyarthralgia, petechiae and palpable purpura accompanied by livedoid change on his legs and feet. Histopathological findings of the purpura revealed perivascular infiltration of neutrophils, mononuclear cells, and nuclear debris, and extravasation of red cells mainly in the upper dermis: all signs consistent with leukocytoclastic vasculitis. Small vessel thrombi, which are characteristic features of septic vasculopathy, were also observed. Direct immunofluorescence showed negative results. Blood culture revealed the growth of gram-negative bacilli. Subsequently, 16S rRNA sequencing of DNA confirmed the organism as Streptobacillus moniliformis, which is the causative pathogen of rat-bite fever. He had frequently encountered wild rats in his house although there was no evidence of rat bite on his body. Empiric therapy with intravenous administration of ceftriaxone in combination with azithromycin hydrate led to a prompt resolution of the symptoms. Precise history-taking related to contact with rats and detection of skin eruptions suggestive of leukocytoclastic vasculitis on the extremities, especially on the feet, can be clues to Streptobacillus moniliformis infection. Familiarity with its cutaneous features is important for early diagnosis; the evidence herein may also help in understanding its underlying pathogenesis.
en-copyright=
kn-copyright=

en-aut-name=KawakamiYoshio
en-aut-sei=Kawakami
en-aut-mei=Yoshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KatayamaTakashi
en-aut-sei=Katayama
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KishidaMasayuki
en-aut-sei=Kishida
en-aut-mei=Masayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OdaWakako
en-aut-sei=Oda
en-aut-mei=Wakako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=InoueYasuro
en-aut-sei=Inoue
en-aut-mei=Yasuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Departments of Dermatology, Okayama City Hospital
kn-affil=

affil-num=2
en-affil=Departments of General Medicine, Okayama City Hospital
kn-affil=

affil-num=3
en-affil=Departments of General Medicine, Okayama City Hospital
kn-affil=

affil-num=4
en-affil=Departments of Pathology, Okayama City Hospital
kn-affil=

affil-num=5
en-affil=Departments of Clinical Laboratory, Okayama City Hospital
kn-affil=
en-keyword=livedo
kn-keyword=livedo
en-keyword=vasculitis
kn-keyword=vasculitis
en-keyword=rat bite fever
kn-keyword=rat bite fever
en-keyword=polyarteritis nodosa
kn-keyword=polyarteritis nodosa
en-keyword=septic vasculopathy
kn-keyword=septic vasculopathy
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=好中球エラスターゼ阻害薬は体外肺灌流システム中の肺機能を改善する
kn-title=A neutrophil elastase inhibitor improves lung function during ex vivo lung perfusion
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=HaradaMasaki
en-aut-sei=Harada
en-aut-mei=Masaki
kn-aut-name=原田昌明
kn-aut-sei=原田
kn-aut-mei=昌明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=6
article-no=
start-page=349
end-page=361
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=201412
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Protective Effect of Eicosapentaenoic Acid on Insulin Resistance in Hyperlipidemic Patients and on the Postoperative Course of Cardiac Surgery Patients: The Possible Involvement of Adiponectin
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Accumulated studies have shown that ω-3 polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) have protective roles against inflammatory responses such as hyperlipidemia, diabetes mellitus (DM) and cardiovascular diseases. Here we examined the effects of administering EPA to hyperlipidemic patients and other patients undergoing cardiac surgery to determine whether this treatment would increase plasma EPA levels and to clarify the association between EPA treatment and adiponectin production in hyperlipidemic patients. We also assessed the effect of preoperative EPA administration on postoperative adverse events such as postoperative atrial fibrillation (POAF) and postoperative infection in the cardiac surgery patients. The EPA administration significantly increased the serum EPA concentrations in both patient populations (p＜0.001). In the hyperlipidemic patients, the EPA administration significantly increased plasma adiponectin levels (p＜0.05), accompanied by a decrease in insulin resistance designated by the HOMA-IR (homeostasis model assessment of insulin resistance) score (p＜0.05) and Hs-CRP (high sensitivity C-reactive protein) value (p＜0.05). In the cardiac surgery patients, no significant effect of EPA on cardiac adverse events such as POAF was observed. However, our results clearly demonstrated that both the neutrophil-to-lymphocyte ratio and the 2nd-line antibiotic requirement in the EPA group were significantly decreased compared to the untreated control group (p＜0.05). We suggest that EPA administration may exert anti-inflammatory effects in patients with hyperlipidemia and in those undergoing cardiac surgery, possibly through an increase in plasma adiponectin levels.
en-copyright=
kn-copyright=

en-aut-name=YamamotoTsuyoshi
en-aut-sei=Yamamoto
en-aut-mei=Tsuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KajikawaYutaka
en-aut-sei=Kajikawa
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OtaniSatoru
en-aut-sei=Otani
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YamadaYuki
en-aut-sei=Yamada
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakemotoSyunji
en-aut-sei=Takemoto
en-aut-mei=Syunji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HirotaMinoru
en-aut-sei=Hirota
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=IkedaMasae
en-aut-sei=Ikeda
en-aut-mei=Masae
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IwagakiHiromi
en-aut-sei=Iwagaki
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SaitoShinya
en-aut-sei=Saito
en-aut-mei=Shinya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=Iwakuni Clinical Center, National Hospital Organization

affil-num=2
en-affil=
kn-affil=Fukuyama Medical Center,National Hospital Organization

affil-num=3
en-affil=
kn-affil=Iwakuni Clinical Center, National Hospital Organization

affil-num=4
en-affil=
kn-affil=Iwakuni Clinical Center, National Hospital Organization

affil-num=5
en-affil=
kn-affil=Fukuyama Medical Center,National Hospital Organization

affil-num=6
en-affil=
kn-affil=Fukuyama Medical Center,National Hospital Organization

affil-num=7
en-affil=
kn-affil=Fukuyama Medical Center,National Hospital Organization

affil-num=8
en-affil=
kn-affil=Fukuyama Medical Center,National Hospital Organization

affil-num=9
en-affil=
kn-affil=Graduate School of Health Sciences, Okayama University

affil-num=10
en-affil=
kn-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
en-keyword=eicosapentaenoic acid
kn-keyword=eicosapentaenoic acid
en-keyword=adiponectin
kn-keyword=adiponectin
en-keyword=hyperlipidemic patients
kn-keyword=hyperlipidemic patients
en-keyword=cardiac surgery
kn-keyword=cardiac surgery
en-keyword=atrial fibrillation
kn-keyword=atrial fibrillation
END

start-ver=1.4
cd-journal=joma
no-vol=17
cd-vols=
no-issue=1
article-no=
start-page=51
end-page=58
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201302
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Current status of the treatment of microscopic polyangiitis and granulomatosis with polyangiitis in Japan
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study aimed to describe the epidemiologic characteristics of microscopic polyangiitis (MPA) and granulomatosis with polyangiitis (GPA) in Japan. 

We used the database of the Ministry of Health, Labour and Welfare (MHLW) from 2006 to 2008, and analyzed data from 938 patients (MPA = 697, GPA = 241) who fulfilled the MHLW diagnostic criteria and had registered within a year after onset. 

The mean ages of the MPA and GPA patients were 69.4 +/- A 0.4 and 58.4 +/- A 1.1 years, respectively. Renal (86.9 %), chest (73.7 %), and nervous system (45.2 %) symptoms were common in MPA patients. Ear, nose, and throat (86.7 %), chest (78.0 %), and renal (60.6 %) symptoms were frequently observed in GPA patients. The concomitant use of cyclophosphamide (CY) with corticosteroids was observed in 22.2 % of the MPA patients and 58.5 % of the GPA patients. In multivariate analysis, the concomitant use of CY was associated with a younger age and pulmonary hemorrhage in MPA patients, and the avoidance of CY was associated with nervous system symptoms and rapidly progressive glomerulonephritis in GPA patients. Plasma exchanges were inducted in 5.2 % of the MPA patients and 4.1 % of the GPA patients. The addition of plasma exchange was associated with elevation of the serum creatinine level in patients with both MPA and GPA. 

A dominance of MPA and a reduced frequency of renal involvement in GPA patients may be significant features of the Japanese population. Clinical practice relating to MPA and GPA in Japan can be characterized as follows: CY is used less commonly, and plasma exchange is employed for patients with deteriorated renal function.
en-copyright=
kn-copyright=

en-aut-name=SugiyamaKoichi
en-aut-sei=Sugiyama
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SadaKen-ei
en-aut-sei=Sada
en-aut-mei=Ken-ei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurosawaMichiko
en-aut-sei=Kurosawa
en-aut-mei=Michiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MakinoHirofumi
en-aut-sei=Makino
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci

affil-num=2
en-affil=
kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci

affil-num=3
en-affil=
kn-affil=Juntendo Univ, Sch Med, Dept Epidemiol & Environm Hlth

affil-num=4
en-affil=
kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci

affil-num=5
en-affil=
kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci
en-keyword=Anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV)
kn-keyword=Anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV)
en-keyword=Cyclophosphamide
kn-keyword=Cyclophosphamide
en-keyword=Microscopic polyangiitis (MPA)
kn-keyword=Microscopic polyangiitis (MPA)
en-keyword=Plasma exchange
kn-keyword=Plasma exchange
en-keyword=Granulomatosis with polyangiitis (GPA)
kn-keyword=Granulomatosis with polyangiitis (GPA)
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130120
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=IL-17A is essential to the development of elastase-induced pulmonary inflammation and emphysema in mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Pulmonary emphysema is characterized by alveolar destruction and persistent inflammation of the airways. Although IL-17A contributes to many chronic inflammatory diseases, it's role in the inflammatory response of elastase-induced emphysema remains unclear. 

Methods: In a model of elastase-induced pulmonary emphysema we examined the response of IL-17A-deficient mice, monitoring airway inflammation, static compliance, lung histology and levels of neutrophil-related chemokine and pro-inflammatory cytokines in bronchoalveolar lavage (BAL) fluid. 

Results: Wild-type mice developed emphysematous changes in the lung tissue on day 21 after elastase treatment, whereas emphysematous changes were decreased in IL-17A-deficient mice compared to wild-type mice. Neutrophilia in BAL fluid, seen in elastase-treated wild-type mice, was reduced in elastase-treated IL-17A-deficient mice on day 4, associated with decreased levels of KC, MIP-2 and IL-1 beta. Elastase-treated wild-type mice showed increased IL-17A levels as well as increased numbers of IL-17A+ CD4 T cells in the lung in the initial period following elastase treatment. 

Conclusions: These data identify the important contribution of IL-17A in the development of elastase-induced pulmonary inflammation and emphysema. Targeting IL-17A in emphysema may be a potential therapeutic strategy for delaying disease progression.
en-copyright=
kn-copyright=

en-aut-name=KurimotoEtsuko
en-aut-sei=Kurimoto
en-aut-mei=Etsuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyaharaNobuaki
en-aut-sei=Miyahara
en-aut-mei=Nobuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KanehiroArihiko
en-aut-sei=Kanehiro
en-aut-mei=Arihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WasedaKoichi
en-aut-sei=Waseda
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TaniguchiAkihiko
en-aut-sei=Taniguchi
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=IkedaGenyo
en-aut-sei=Ikeda
en-aut-mei=Genyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KogaHikari
en-aut-sei=Koga
en-aut-mei=Hikari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NishimoriHisakazu
en-aut-sei=Nishimori
en-aut-mei=Hisakazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TanimotoYasushi
en-aut-sei=Tanimoto
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KataokaMikio
en-aut-sei=Kataoka
en-aut-mei=Mikio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=IwakuraYoichiro
en-aut-sei=Iwakura
en-aut-mei=Yoichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=GelfandErwin W.
en-aut-sei=Gelfand
en-aut-mei=Erwin W.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=TanimotoMitsune
en-aut-sei=Tanimoto
en-aut-mei=Mitsune
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=2
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=4
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=6
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=7
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=8
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=9
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=10
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med

affil-num=11
en-affil=
kn-affil=Univ Tokyo, Inst Med Sci, Ctr Expt Med & Syst Biol

affil-num=12
en-affil=
kn-affil=Natl Jewish Hlth, Dept Pediat, Div Cell Biol

affil-num=13
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Hematol Oncol Allergy & Resp Med
en-keyword=IL-17
kn-keyword=IL-17
en-keyword=Elastase
kn-keyword=Elastase
en-keyword=Emphysema
kn-keyword=Emphysema
en-keyword=Chronic obstructive pulmonary disease
kn-keyword=Chronic obstructive pulmonary disease
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130124
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Inhibition of neutrophil elastase attenuates airway hyperresponsiveness and inflammation in a mouse model of secondary allergen challenge: neutrophil elastase inhibition attenuates allergic airway responses
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Chronic asthma is often associated with neutrophilic infiltration in the airways. Neutrophils contain elastase, a potent secretagogue in the airways, nonetheless the role for neutrophil elastase as well as neutrophilic inflammation in allergen-induced airway responses is not well defined. In this study, we have investigated the impact of neutrophil elastase inhibition on the development of allergic airway inflammation and airway hyperresponsiveness (AHR) in previously sensitized and challenged mice. 

Methods: BALB/c mice were sensitized and challenged (primary) with ovalbumin (OVA). Six weeks later, a single OVA aerosol (secondary challenge) was delivered and airway inflammation and airway responses were monitored 6 and 48 hrs later. An inhibitor of neutrophil elastase was administered prior to secondary challenge. 

Results: Mice developed a two-phase airway inflammatory response after secondary allergen challenge, one neutrophilic at 6 hr and the other eosinophilic, at 48 hr. PAR-2 expression in the lung tissues was enhanced following secondary challenge, and that PAR-2 intracellular expression on peribronchial lymph node (PBLN) T cells was also increased following allergen challenge of sensitized mice. Inhibition of neutrophil elastase significantly attenuated AHR, goblet cell metaplasia, and inflammatory cell accumulation in the airways following secondary OVA challenge. Levels of IL-4, IL-5 and IL-13, and eotaxin in BAL fluid 6 hr after secondary allergen challenge were significantly suppressed by the treatment. At 48 hr, treatment with the neutrophil elastase inhibitor significantly reduced the levels of IL-13 and TGF-beta 1 in the BAL fluid. In parallel, in vitro IL-13 production was significantly inhibited in spleen cells from sensitized mice. 

Conclusion: These data indicate that neutrophil elastase plays an important role in the development of allergic airway inflammation and hyperresponsiveness, and would suggest that the neutrophil elastase inhibitor reduced AHR to inhaled methacholine indicating the potential for its use as a modulator of the immune/inflammatory response in both the neutrophil-and eosinophil-dominant phases of the response to secondary allergen challenge.
en-copyright=
kn-copyright=

en-aut-name=KogaHikari
en-aut-sei=Koga
en-aut-mei=Hikari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyaharaNobuaki
en-aut-sei=Miyahara
en-aut-mei=Nobuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FuchimotoYasuko
en-aut-sei=Fuchimoto
en-aut-mei=Yasuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IkedaGenyo
en-aut-sei=Ikeda
en-aut-mei=Genyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WasedaKoichi
en-aut-sei=Waseda
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OnoKatsuichiro
en-aut-sei=Ono
en-aut-mei=Katsuichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TanimotoYasushi
en-aut-sei=Tanimoto
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KataokaMikio
en-aut-sei=Kataoka
en-aut-mei=Mikio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=GelfandErwin W.
en-aut-sei=Gelfand
en-aut-mei=Erwin W.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=TanimotoMitsune
en-aut-sei=Tanimoto
en-aut-mei=Mitsune
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KanehiroArihiko
en-aut-sei=Kanehiro
en-aut-mei=Arihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=2
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=3
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=4
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=5
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=6
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=7
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=8
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=9
en-affil=
kn-affil=Natl Jewish Hlth, Dept Pediat, Div Cell Biol

affil-num=10
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci

affil-num=11
en-affil=
kn-affil=Okayama Univ, Dept Hematol Oncol Allergy & Resp Med, Grad Sch Med Dent & Pharmaceut Sci
en-keyword=Neutrophil
kn-keyword=Neutrophil
en-keyword=Elastase
kn-keyword=Elastase
en-keyword=Airway
kn-keyword=Airway
en-keyword=Hyperresponsiveness
kn-keyword=Hyperresponsiveness
en-keyword=Asthma
kn-keyword=Asthma
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=好中球エラスターゼの阻害はマウスモデルにおいてセカンダリーアレルゲンチャレンジによる気道過敏性と気道炎症：アレルギー性気道反応を抑制する
kn-title=Inhibition of neutrophil elastase attenuates airway hyperresponsiveness and inflammation in a mouse model of secondary allergen challenge: neutrophil elastase inhibition attenuates allergic airway responses
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=KogaHikari
en-aut-sei=Koga
en-aut-mei=Hikari
kn-aut-name=古賀光
kn-aut-sei=古賀
kn-aut-mei=光
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END

start-ver=1.4
cd-journal=joma
no-vol=125
cd-vols=
no-issue=2
article-no=
start-page=109
end-page=112
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130801
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Therapeutic effects of redox-active protein thioredoxin(TRX)-1 in influenza-virus-induced pneumonia in mice
kn-title=マウスインフルエンザ肺炎におけるレドックス制御蛋白チオレドキシン（TRX-1）の治療的効果
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=YashiroMasato
en-aut-sei=Yashiro
en-aut-mei=Masato
kn-aut-name=八代将登
kn-aut-sei=八代
kn-aut-mei=将登
aut-affil-num=1
ORCID=

en-aut-name=TsukaharaHirokazu
en-aut-sei=Tsukahara
en-aut-mei=Hirokazu
kn-aut-name=塚原宏一
kn-aut-sei=塚原
kn-aut-mei=宏一
aut-affil-num=2
ORCID=

en-aut-name=MatsukawaAkihiro
en-aut-sei=Matsukawa
en-aut-mei=Akihiro
kn-aut-name=松川昭博
kn-aut-sei=松川
kn-aut-mei=昭博
aut-affil-num=3
ORCID=

en-aut-name=YamadaMutsuko
en-aut-sei=Yamada
en-aut-mei=Mutsuko
kn-aut-name=山田睦子
kn-aut-sei=山田
kn-aut-mei=睦子
aut-affil-num=4
ORCID=

en-aut-name=FujiiYosuke
en-aut-sei=Fujii
en-aut-mei=Yosuke
kn-aut-name=藤井洋輔
kn-aut-sei=藤井
kn-aut-mei=洋輔
aut-affil-num=5
ORCID=

en-aut-name=NagaokaYoshiharu
en-aut-sei=Nagaoka
en-aut-mei=Yoshiharu
kn-aut-name=長岡義晴
kn-aut-sei=長岡
kn-aut-mei=義晴
aut-affil-num=6
ORCID=

en-aut-name=TsugeMitsuru
en-aut-sei=Tsuge
en-aut-mei=Mitsuru
kn-aut-name=津下充
kn-aut-sei=津下
kn-aut-mei=充
aut-affil-num=7
ORCID=

en-aut-name=YamashitaNobuko
en-aut-sei=Yamashita
en-aut-mei=Nobuko
kn-aut-name=山下信子
kn-aut-sei=山下
kn-aut-mei=信子
aut-affil-num=8
ORCID=

en-aut-name=ItoToshihiro
en-aut-sei=Ito
en-aut-mei=Toshihiro
kn-aut-name=伊藤利洋
kn-aut-sei=伊藤
kn-aut-mei=利洋
aut-affil-num=9
ORCID=

en-aut-name=YamadaMasao
en-aut-sei=Yamada
en-aut-mei=Masao
kn-aut-name=山田雅夫
kn-aut-sei=山田
kn-aut-mei=雅夫
aut-affil-num=10
ORCID=

en-aut-name=MasutaniHiroshi
en-aut-sei=Masutani
en-aut-mei=Hiroshi
kn-aut-name=増谷弘
kn-aut-sei=増谷
kn-aut-mei=弘
aut-affil-num=11
ORCID=

en-aut-name=YodoiJunji
en-aut-sei=Yodoi
en-aut-mei=Junji
kn-aut-name=淀井淳司
kn-aut-sei=淀井
kn-aut-mei=淳司
aut-affil-num=12
ORCID=

en-aut-name=MorishimaTsuneo
en-aut-sei=Morishima
en-aut-mei=Tsuneo
kn-aut-name=森島恒雄
kn-aut-sei=森島
kn-aut-mei=恒雄
aut-affil-num=13
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学

affil-num=2
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 病理学（免疫病理）

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学

affil-num=5
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学

affil-num=6
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学

affil-num=7
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学

affil-num=8
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学

affil-num=9
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 病理学（免疫病理）

affil-num=10
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 病原ウイルス学

affil-num=11
en-affil=
kn-affil=京都大学ウイルス研究所 生体応答学研究部門

affil-num=12
en-affil=
kn-affil=京都大学ウイルス研究所 生体応答学研究部門

affil-num=13
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 小児医科学
en-keyword=acute lung injury
kn-keyword=acute lung injury
en-keyword=cytokine
kn-keyword=cytokine
en-keyword=influenza virus
kn-keyword=influenza virus
en-keyword=oxidative stress
kn-keyword=oxidative stress
en-keyword=thioredoxin-1
kn-keyword=thioredoxin-1
END

start-ver=1.4
cd-journal=joma
no-vol=4
cd-vols=
no-issue=6
article-no=
start-page=572
end-page=576
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200811
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Hemin Treatment Abrogates Monocrotaline-Induced Pulmonary Hypertension
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Treatment of rats with monocrotaline (MCT), a pyrrolizidine alkaloid plant toxin, is known to cause pulmonary hypertension (PH), and it has been used as a useful experimental model of PH. Recent findings suggested that pulmonary inflammation may play a significant role in the pathogenesis of MCT-induced PH. We also demonstrated that, following MCT administration to rats, there was a significant and sustained increase in the pulmonary expression of heme oxygenase-1 (HO-1), which is known to be induced by various oxidative stresses, including inflammation and free heme, and is thought to be essential in the protection against oxidative tissue injuries. In this study, we administered hemin (ferriprotoporphyrin chloride, 30 mol/kg b.w., subcutaneously), a potent inducer of HO-1, every 3 days to rats following subcutaneous administration of MCT (60 mg/kg) and examined its effect on MCT-induced PH and pulmonary inflammation. MCT administration caused pulmonary arterial wall thickening with marked elevation of right ventricular pressure, in association with prominent pulmonary inflammation as revealed by the increase in gene expression of tumor necrosis factor-alpha and the number of infiltrated neutrophils in the lung. In contrast, hemin treatment of MCT-administered animals, which led to a further increase in pulmonary HO-1 mRNA expression, significantly ameliorated MCT-induced PH as well as tissue inflammation. These findings suggest that hemin treatment ameliorates MCT-induced PH possibly mediated through induction of pulmonary HO-1 which leads to the attenuation of pulmonary inflammation.
en-copyright=
kn-copyright=

en-aut-name=ShimzuK.
en-aut-sei=Shimzu
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakahashiT.
en-aut-sei=Takahashi
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=IwasakiT.
en-aut-sei=Iwasaki
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ShimizuH.
en-aut-sei=Shimizu
en-aut-mei=H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=InoueK.
en-aut-sei=Inoue
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MorimatsuH.
en-aut-sei=Morimatsu
en-aut-mei=H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OmoriE.
en-aut-sei=Omori
en-aut-mei=E.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MatsumiM.
en-aut-sei=Matsumi
en-aut-mei=M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=AkagiR.
en-aut-sei=Akagi
en-aut-mei=R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MoritaK.
en-aut-sei=Morita
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=2
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=4
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=6
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=7
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=8
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol

affil-num=9
en-affil=
kn-affil=Okayama Prefectural Univ, Dept Nutr Sci

affil-num=10
en-affil=
kn-affil=Okayama Univ, Grad Sch Med & Dent, Dept Anesthesiol & Resuscitol
en-keyword=Heme oxygenase-1
kn-keyword=Heme oxygenase-1
en-keyword=hemin
kn-keyword=hemin
en-keyword=inflammation
kn-keyword=inflammation
en-keyword=monocrotaline
kn-keyword=monocrotaline
en-keyword=pulmonary hypertension
kn-keyword=pulmonary hypertension
en-keyword=tumor necrosis factor-alpha
kn-keyword=tumor necrosis factor-alpha
END

start-ver=1.4
cd-journal=joma
no-vol=41
cd-vols=
no-issue=1
article-no=
start-page=171
end-page=181
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201301
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Redox-Active Protein Thioredoxin-1 Administration Ameliorates Influenza A Virus (H1N1)-Induced Acute Lung Injury in Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Objectives: Influenza virus infections can cause severe acute lung injury leading to significant morbidity and mortality. Thioredoxin-1 is a redox-active defensive protein induced in response to stress conditions. Animal experiments have revealed that thioredoxin-1 has protective effects against various severe disorders. This study was undertaken to evaluate the protective effects of recombinant human thioredoxin-1 administration on influenza A virus (H1N1)-induced acute lung injury in mice. 

Design: Prospective animal trial. 

Setting: Research laboratory. 

Subjects: Nine-week-old male C57BL/6 mice inoculated with H1N1. 

Intervention: The mice were divided into a vehicle-treated group and recombinant human thioredoxin-1-treated group. For survival rate analysis, the vehicle or recombinant human thioredoxin-1 was administered intraperitoneally every second day from day -1 to day 13. For lung lavage and pathological analyses, vehicle or recombinant human thioredoxin-1 was administered intraperitoneally on days 1, 1, and 3. 

Measurements and Main Results: Lung lavage and pathological analyses were performed at 24, 72, and 120 hrs after inoculation. The recombinant human thioredoxin-1 treatment significantly improved the survival rate of H1N1-inoculated mice, although the treatment did not affect virus propagation in the lung. The treatment significantly attenuated the histological changes and neutrophil infiltration in the lung of H1N1-inoculated mice. The treatment significantly attenuated the production of tumor necrosis factor-a and chemokine (C-X-C motif) ligand 1 in the lung and oxidative stress enhancement, which were observed in H1N1-inoculated mice. H1N1 induced expressions of tumor necrosis factor-a and chemokine (C-X-C motif) ligand 1 in murine lung epithelial cells MLE-12, which were inhibited by the addition of recombinant human thioredoxin-1. The recombinant human thioredoxin-1 treatment started 30 mins after H1N1 inoculation also significantly improved the survival of the mice. 

Conclusions: Exogenous administration of recombinant human thioredoxin-1 significantly improved the survival rate and attenuated lung histological changes in the murine model of influenza pneumonia. The protective mechanism of thioredoxin-1 might be explained by its potent antioxidative and anti-inflammatory actions. Consequently, recombinant human thioredoxin-1 might be a possible pharmacological strategy for severe influenza virus infection in humans. (Crit Care Med 2013; 41:171-181)
en-copyright=
kn-copyright=

en-aut-name=YashiroMasato
en-aut-sei=Yashiro
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsukaharaHirokazu
en-aut-sei=Tsukahara
en-aut-mei=Hirokazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MatsukawaAkihiro
en-aut-sei=Matsukawa
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YamadaMutsuko
en-aut-sei=Yamada
en-aut-mei=Mutsuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=FujiiYosuke
en-aut-sei=Fujii
en-aut-mei=Yosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NagaokaYoshiharu
en-aut-sei=Nagaoka
en-aut-mei=Yoshiharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TsugeMitsuru
en-aut-sei=Tsuge
en-aut-mei=Mitsuru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YamashitaNobuko
en-aut-sei=Yamashita
en-aut-mei=Nobuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=ItoToshihiro
en-aut-sei=Ito
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=YamadaMasao
en-aut-sei=Yamada
en-aut-mei=Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=MasutaniHiroshi
en-aut-sei=Masutani
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pediat

affil-num=2
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pediat

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pathol & Expt Med

affil-num=4
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pediat

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pediat

affil-num=6
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pediat

affil-num=7
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pediat

affil-num=8
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pediat

affil-num=9
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pathol & Expt Med

affil-num=10
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Virol

affil-num=11
en-affil=
kn-affil=Kyoto Univ, Inst Virus Res, Dept Biol Responses
en-keyword=acute lung injury
kn-keyword=acute lung injury
en-keyword=cytokine
kn-keyword=cytokine
en-keyword=influenza virus
kn-keyword=influenza virus
en-keyword=mouse
kn-keyword=mouse
en-keyword=oxidative stress
kn-keyword=oxidative stress
en-keyword=thioredoxin-1
kn-keyword=thioredoxin-1
END

start-ver=1.4
cd-journal=joma
no-vol=66
cd-vols=
no-issue=4
article-no=
start-page=317
end-page=327
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201208
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Water Extract of Vitis coignetiae Pulliat Leaves Attenuates Oxidative Stress and Inflammation in Progressive NASH Rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study aimed to investigate the therapeutic effects of the water extract of leaves of Vitis coignetiae
Pulliat (VCPL) on nonalcoholic steatohepatitis (NASH) with advanced fibrosis, as our previous study exhibited its preventive effect on NASH. The NASH animal model [PCT/JP2007/52477] was prepared by loading recurrent and intermittent hypoxemia stress to a rat with fatty liver, which resembled the condition occurring in patients with obstructive sleep apnea (OSA) and fatty liver, who have a high incidence of NASH. Intermittent hypoxemia stress is regarded as a condition similar to warm ischemia followed by re-oxygenation, which induces oxidative stress (OS). The daily 100 or 300mg/kg VCPL administrations were performed for 3 weeks perorally beginning at the time of detection of advanced liver fibrosis. The therapeutic efficacy of VCPL on NASH was demonstrated by the reduction of the leakage of hepato-biliary enzymes and the amelioration of liver fibrosis. The OS elevation in NASH rats was measured based on the derivation of reactive oxygen species from liver mitochondrial energy metabolism and on the decrease in plasma SOD-like activity. The aggravation of inflammatory responses was demonstrated by the neutrophil infiltration (elevated myeloperoxidase activity) and the progression of fibrosis in the livers of NASH rats. In addition, the NASH rats without
VCPL treatment also exhibited activation of nuclear factor-κB, a key factor in the link between oxidative stress and inflammation. All of these changes were reduced dose-dependently by the VCPL administration. These findings indicate that VCPL may improve hepatic fibrosis or at least suppress the progression of NASH, by breaking the crosstalk between OS and inflammation.
en-copyright=
kn-copyright=

en-aut-name=PakWing
en-aut-sei=Pak
en-aut-mei=Wing
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakayamaFusako
en-aut-sei=Takayama
en-aut-mei=Fusako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HasegawaAzusa
en-aut-sei=Hasegawa
en-aut-mei=Azusa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MankuraMitsumasa
en-aut-sei=Mankura
en-aut-mei=Mitsumasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=EgashiraToru
en-aut-sei=Egashira
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=UekiKeiji
en-aut-sei=Ueki
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NakamotoKazuo
en-aut-sei=Nakamoto
en-aut-mei=Kazuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KawasakiHiromu
en-aut-sei=Kawasaki
en-aut-mei=Hiromu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=MoriAkitane
en-aut-sei=Mori
en-aut-mei=Akitane
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Clinical Pharmaceutical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=2
en-affil=
kn-affil=Department of Clinical Pharmaceutical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=3
en-affil=
kn-affil=Department of Clinical Pharmaceutical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=4
en-affil=
kn-affil=Department of Anti-Aging Food Sciences, Okayama University Graduate School of Medicine

affil-num=5
en-affil=
kn-affil=Department of Clinical Pharmaceutical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=6
en-affil=
kn-affil=Hiruzen Winery Co., Ltd

affil-num=7
en-affil=
kn-affil=Department of Clinical Pharmaceutical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=8
en-affil=
kn-affil=Department of Clinical Pharmaceutical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=9
en-affil=
kn-affil=Department of Anti-Aging Food Sciences, Okayama University Graduate School of Medicine
en-keyword=non-alcoholic steatohepatitis
kn-keyword=non-alcoholic steatohepatitis
en-keyword=antioxidative
kn-keyword=antioxidative
en-keyword=oxidative stress
kn-keyword=oxidative stress
en-keyword=anti-inflammation
kn-keyword=anti-inflammation
en-keyword=Vitis coignetiae Pulliat
kn-keyword=Vitis coignetiae Pulliat
END

start-ver=1.4
cd-journal=joma
no-vol=43
cd-vols=
no-issue=1
article-no=
start-page=18
end-page=26
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=1994
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Effect of Endotoxin on the Neutrophil Chemiluminescence in the Rabbit
kn-title=エンドトキシン血症家兎の好中球化学発光の変動
en-subtitle=
kn-subtitle=
en-abstract=To examine the effects of endotoxin on the neutrophil function, neutrophil-dependent microbicidal activities were evaluated by the chemiluminescence (CL) method in rabbits injected with endotoxin. Six rabbits in the first group were administered saline intravenously as control. Ten rabbits each in the second and third group received endotoxin 0.7 μg・kg(-1) and 70 μg・kg(-1) respectively. Luminol-dependent CL in whole blood (whole blood CL) and in isolated neutrophils (neutrophil CL) were examined before administration and 30, 60, 180 and 360 min after administration. Peak values of whole blood CL in the second group were significantly depressed at 30 and 60 min, and elevated at 360 min. Those in the third group were depressed more than in the second group at all times after endotoxin administration. Peak values of neutrophil CL in the second group at 180 and 360 min were significantly elevated, but those in the third group at 30, 60, 180 min were markedly depressed. Experiments combining neutrophils and pooled plasma revealed that these CL responses were associated with the neutrophils themselves. It is speculated that the neutrophil supression observed in the third group was caused by the overactivation of ncutrophils induced by the activated complements and some other stimulating factors, and/or may be due to the abundant appearance of immature granulocytes with lesser activities.
kn-abstract=好中球の貪食殺菌能を調べる方法として，増感剤を用いた化学発光(CL)法がある。好中球CLは細菌感染によって増大するが,重症感染症になると低下する場合がある。重症の細菌感染症における好中球CLの低下の原因を探るために，家兎に高濃度(70μg･kg(-1):III群)あるいは低濃度エンドトキシン(0.7μg･kg(-1):II群)を一回静注し，好中球の貪食殺菌能をCL法によって経時的に測定した。好中球を用いたCLピーク値は，II群では180分，360分で有意の上昇が認められ，III群では30分，60分，180分で有意の低下が認められた。好中球とプール血漿を組み合わせてCLを測定したところ，類似した結果が得られたため，好中球側に原因があることが示唆された。全血を用いたCLピーク値と白血球数とは類似の変化を示し，30分で有意に低下し，60分で最低を示したのちに回復した。以上より,軽度のエンドトキシン血症時には，好中球自体の貪食殺菌能が亢進する。しかし，重症エンドトキシン血症時には好中球数減少に伴う生体側の殺菌能の低下のほかに，好中球自体の貪食殺菌能の抑制が起こることが示された。
en-copyright=
kn-copyright=

en-aut-name=AsaoYasuhiro
en-aut-sei=Asao
en-aut-mei=Yasuhiro
kn-aut-name=浅雄保宏
kn-aut-sei=浅雄
kn-aut-mei=保宏
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部麻酔･蘇生学教室
END

start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=8
article-no=
start-page=1237
end-page=1267
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1927
dt-pub=19270831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimentelle Studie ?ber den Ikterus. (2. Mitteilung.) Ueber die Blutbefunde bei der Spiroch?tosis ictero-h?morrhagica und vergleichenden Resultate jedes Blutbefundes bei der Spiroch?tosis ictero-h?morrhagica, des Stauungsikterus, und desjenigen Ikterus, der durch die Injektion von Toluylendiamin herbeigefuhrt wurde
kn-title=黄疸ニ關スル實驗的研究（第二囘報告） 黄疸出血性「スピロヘータ」病ノ血液所見ニ關スル實驗的研究 附 鬱滯性竝ニ「トルイレンヂアミン」黄疸ノ血液所見トノ比較
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Die Verfasser untersuchten die Blutbefunde bei der Spiroch?tosis ictero-h?morrhagica, dem Stauungsikterus und demjenigen Ikterus, der durch die Injektion von Toluylendiamin herbeigef?hrt wurde, und beobachteten die Beziehungen der Entstehungsweise des Ikterus bei der Spiroch?tosis ictero-h?morrhagica auf die Blutbefunde bei den drei obenerw?hnten Arten von Ikterus. Als Versuchsmaterial benutzten wir die Hunde, Meerschweinchen, und Kaninchen.
Aus den Resultaten haben wir die folgenden Schluesse gezogen: 1. Die Zahl der Erythrozyten vermindert rich ja sicher bei der Spiroch?tosis icteroh?morrhagica mit dem Ausbruch derselben und besonders ist es am deutlichsten unmittelbar vor dem Ausscheinen des Ikterus, dagegen beim Stauungsikterus nimmt sie je mehr mit der Aufsteigung des Ikterus ab. Beim Toluyleudiaminikterus folgt sich eine sehr auffallendpl?tzliche verminderung in 24-48 Stunden nach der Injektion. 2. Der Faerbeiudex wird bei der Spiroch?tosis ictero-h?morrhagica von jedem Krankheitstadium kaum beeinflusst. Beim Stauungsikterus nimmt er parallel mit dem H?moglobingehalt ab., und beim Toluylendiaminikterus vermindert sich er am deutlichsten in 24-48 Stunden nach der Injektion und dann mit dem Verschwinden des Ikterus in den fr?heren Zustand zur?ck. 3. Die Zahl der Leukozyten vermehrt sich bei der Spiroch?tosis ictero-h?morrhagica allmaehlich im Verlaufe der Krankheit, besonders findet man dabei bedeutende vermehrung der Polymorphkernigen ueutrophylen Leukozyten, beim Stauungsikterus wird sie wenig beeinflusst. Beim Toluylendiaminikterus vermehrt sie sich am deutlichsten in 24-48 Stunden nach der Injektion, besonders Polymorphkernige neutrophile Leuko-zyten. 4. Der Bilirubingehalt im Bluteserum vermehrt sich bei der Spiroch?tosis ikteroh?morrhagica pl?tzlich direkt nach dem Ausbruch des Ikterus, und die direkte Diazoreaktion zeigt sich meist prompt, beim Stauungsikterus vermehrt er sich allm?hlich, beim Toluylendiaminikterus erreicht er seinen H?hepunkt in 24-48 Stunden nach der Injektion. 5. Die Resistenz der Erythrozyten gegen die hypotonische Kochsalzl?sung ist bei der Spiroch?tosis ictero-h?morrhagica ganz unbestimmt, indem sie sich bei einem erh?ht, beim anderen absteigt, beim Stauungsikterus vermehrt sie sich allm?hlich. Beim Toluylendiaminikterus nimmt sie dagegen in 24-48 Stunden nach der Injektion ab, und kehrt dan in den fr?heren Zustand wieder zurueck. 6. Die Menge des Serumeiweiss vermindert sich bei der Spiroch?tosis ictero-h?morr-hagika gew?hnlich nach und nach. Sowohl beim Stauungsikterus als auch beim Toluylendiaminikterus vermehrt sie sich allm?hlich parallel mit dem Bilirubingehalt im Blutserum. 7. Die Senkungsgeschwindigkeit der Erythrozyten verz?gert sich bei der Spiroch?tosis ictero-h?morrhagica, und zwar im Ikterusstadium, als auch beim Stauungsikterus. Beim Toluylendiaminikterus beschleunigt sie sich dagegen. 8. Aus diesem Tatsachen kommt es heraus, dass der Blutbefund der Spiroch?tosis ictero-h?morrhagica mit dem des Stauungsikterus manche Aehnlichkeit hat.
en-copyright=
kn-copyright=

en-aut-name=HasimotoMasaiti
en-aut-sei=Hasimoto
en-aut-mei=Masaiti
kn-aut-name=橋本政一
kn-aut-sei=橋本
kn-aut-mei=政一
aut-affil-num=1
ORCID=

en-aut-name=KuroseIwao
en-aut-sei=Kurose
en-aut-mei=Iwao
kn-aut-name=黒�P巖
kn-aut-sei=黒�P
kn-aut-mei=巖
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學稻田内科教室

affil-num=2
en-affil=
kn-affil=岡山醫科大學稻田内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=66
cd-vols=
no-issue=10 suppl
article-no=
start-page=1
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1955
dt-pub=19550228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=EXPERIMENTAL STUDIES ON JAPANESE B ENCEPHALITIS. 1. HAEMOGRAMS OF THE MICE INFECTED WITH JAPANESE B ENCEPHALITIS AND THE EFECTS OF RADIOACTVE P(32) ADMINISTRATION
kn-title=日本脳炎に関する実験的研究 第一報 日本脳炎罹患マウスの血液像，特にそれに及ぼす放射性同位元素P(32)の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Comparative studies on the haemograms of the mice infected with Japanese B encephalitis with or without the administration of radioactive P(32) were made. 1. In the haemogram of the infected mouse a decrease in the number of white blood cells was observed along with the duration, although there occurred no appreciable amount of changes in the numbers of red blood cells and reticulocytes and in the quantity of haemoglobin. This drop in the number of while blood cells was especially remarkable during the infection period. The classification of the white blood cells showed an increase in the percentage of neutrophilic leucocytes and a decrease in the absolute number of lymphocytes. 2. The administration of radio active P(32) to the normal mouse caused some haemogramaberrations, proportional to the amounts of doses: When 30 μc. p(32) was given, the numbers of red blood cells and reticulocytes and ihe quantity of haemogIobin remaining the same as usual, the number of white blood cells showed a considerable decrease, its minimum value appearing 4 or 5 days after the admininistration. 40 μc. dose gave rise to a slight decrease in the numbers of red blood.cells and reticuloocytes and in the quantity of haemoglobin, the decrease in white blood cell number being far more remarkable than that caused by 30 μc. administration. In both of these two cases, the increases in the percentage of neutrophilic leucocytes and the decreases in the absolute number of lymphocytes were also observed. 3. 30 μc. P(32) administration to the infected mouse induced no significant changes in the numbers of red blood cells and reticulocytes and in the quantity of haemo gIobin, but the number of leucocytcs showed a more marked decrease than that of the infected mouse with no P(32) administration, the increase in the percentage of neutrophilic leucocytes having been depressed. In addition, the elongation of the latent period for Encephalitis of the mice were recognized.
en-copyright=
kn-copyright=

en-aut-name=HoenYukihiko
en-aut-sei=Hoen
en-aut-mei=Yukihiko
kn-aut-name=方円幸彦
kn-aut-sei=方円
kn-aut-mei=幸彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=40
cd-vols=
no-issue=7
article-no=
start-page=1515
end-page=1527
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1928
dt-pub=19280731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Einige klinische h?matologische Untersuchungen ?ber bie Lungentuberkulose
kn-title=肺結核患者ニ於ケル2, 3ノ血液檢査成績ニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bei 50 F?llen von Phthisikern sind w?hrend des Krankheitsverlaufs genaue h?matologische Untersuchungen von uns vorgenommen und folgende Resultate erzielt worden 1. Bei Lungentuberkulose ist die Blutk?rperchensekungsgeschiwindigkeit ziemlich parallel mit der Zunahme der tuberkul?sen Intoxikation beschleunigt, bei schwereren. F?llen aber, und zwar besonders vor dem Tode zeigt sich gerade das umgeke hrte Verh?ltnis. 2. Im allgemeinen nimmt der Blutviskosit?tswert bis etwas ?ber normal zu gem?ss der Krankheitsverschlimmerung nimmt dieser Wert aber auch ab. 3. mit Ausnahme bei schweren Intoxikationen bleibt die Erythrozytenzahl und der H?moglobingehalt ungef?hr innerhalb der Normalgrenze, aber bei schwereren F?llen zeigt sich bei beiden eine st?rkere Verminderung. 4. Die weisen Blutk?rperchen vermehren ihre Zahl bei schweren Erkrankungen, wobei sich haupts?chlich die neutrophlen polymorphkernigen Leukozyten beteiligen und sich das “neutrophile Blutbild” nach links verschiebt. 5. Die Monozyten sich dabei sehr wenig, aber im allgemeinen zeigen sie eine sehr geringe Abnahme. 6. Mit ber Ausdehnung der Erkrankung und der Verschlechterung des allgemeinen Zustandes parllel geht der Eosinophilenschwund aus den peripheren Blutbahnen; ihr Wiederauftreten h?ngt von der Besserung das Zustandes ab. 7. Der Blutviskosit?tswert ist abh?ngig von dem H?moglobin und der Erythrozytenzahl, aber nicht von der Leukozytenzahl und der Senkungsgescgwindigkeit.
en-copyright=
kn-copyright=

en-aut-name=HaraKatumi
en-aut-sei=Hara
en-aut-mei=Katumi
kn-aut-name=原勝巳
kn-aut-sei=原
kn-aut-mei=勝巳
aut-affil-num=1
ORCID=

en-aut-name=TuyosiTukuda
en-aut-sei=Tuyosi
en-aut-mei=Tukuda
kn-aut-name=佃毅
kn-aut-sei=佃
kn-aut-mei=毅
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學稻田内科教室

affil-num=2
en-affil=
kn-affil=岡山醫科大學稻田内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=40
cd-vols=
no-issue=6
article-no=
start-page=1240
end-page=1256
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1928
dt-pub=19280630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=?ber einen Fall von sog. ?osinophilie pleurale
kn-title=所謂?osinophilie pleuraleニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=?ber Eosiniphilie in Pleuraerg?ssen finden sich in der Literatur nur wenige Angaben und die Frage nach der Entstehung der Eosinophilie ist bisher noch nicht gel?st worden. Neuerdings habe ich Gelegenheit gehabt, eine hochgradige Eosinophilie in einem aseptisch-eiterigen Pleuraexsudat zu beobachten. Der Fall betrifft einen 32-j?hrigen, ziemlich kr?ftig gebauten Mann. Ende Nov. 1927 suchte der Patient die Poliklinik wegen der seit etwa 2 Wochen fortdauernden, stechenden Schmerzen in der linken Brustseite beim Atmen in gleicbnamiger Seitenlage auf. Bei der Untersuchung fanden wir bei sonst normalem Lungenbefund eine leichte Schallverk?rzung in der linken seitlichen Brustgegend. Die R?ntgenuntersuchung zeigte keine besonderen Verschattungen. Die Probepunktion ergab das Vorhandensein eines d?nnfl?ssigen, stark getr?bten Ergusses. Spec. Gew. 1020, Rivalta'sche Reaktion stark positiv, Eiweissgehalt 3%, zytologische Bestandteile: Rote Blutzellen 6%, Neutrophile Lymphozyten 2%, Monozyten 7%, einkernige Eosinophilie 13.5%, zweikernige E. 63.5%, polymorphkernige E. 5.5%. Nach 2 Tagen bekam ich noch 30cc einer tr?ben Fl?ssigkeit, worin man 162400 Blutzellen pro 1cmm fand. Die Zellarten waren folgende: Rote Blutzellen 1%, Neutrophile 0.5%, Lymphozyten 2%, Monozyten 11.5%, einkernige Ensinophile 37%, zweikernige E. 48%, polymorphkernige E. 0.5%. Nach weiteren 6 Tagen wurden noch 30cc herausgenommen. Zellenzahl pro 1cmm 33300, davon 0.5% rote Blutzellen, 0.5% Neutrophile, 0.5% Lymphozyten, 8% Monozyten, 6% einkernige Eosinophile, 64% zweikernige E., 12% polymorphkernige E., 8.5% regressive Formen der Eosinophilen. Nach weiteren 7 Tagen verschwanden die s?mtlichen subjektiven Beschwerden und die Probepunktion zeigte keinen Erfolg mehr. Die Eosinophilen ergaben keine Ver?nderungen an Granula-Beschaffenheit und Kernger?ststruktur, abgesehn von der Verkleinerung der Zellen selbst und dem Vorhandensein des mononukle?ren Typus. Dagegen konnte man in den Monozyten eine mehr oder weniger deutliche Eigent?mlichkeit, ein Phagozytosenbild von acidophilen Granula oder roter Blutzellen selbst, wahrnehmen. Bei dem Kranken fanden wir gleichzeitig eine m?ssig starke Bluteosinophilie mit leichtgradiger Leukozytose, aber weder Neoplasmen noch Parasiteneier in Stuhl und Sputum. Der Patient hatte noch Hyperazidit?t, Hyperpankreatismus, gesteigerten Gaswechsel, vermehrte Harns?ureausscheidung, Pilocarpinempfindlichkeit: kurz, er war sicher. ein Vagotoniker mit den meisten Zeichen von sog. konstitutionellen Eosinophilie KLINKERT. Nach obigen Befunden ist der Verfasser der Meinung, dass bei sog. eosinophiler Pleuritis die auf geringe Reize direkt mit eosinophiler Ent?ndung reagierende Konstitution ?tiologisch eine grosse Rolle spielt. Um diese Annahme zu best?tigen, wurde noch das bekannte Neusser'sche Experiment ausgef?hrt und in k?nstlich erzeugten Vesikansblasen eine merkliche Eosinophilie konstatiert. Demzufolge ist die pleurale Eosinophilie unseres Falles mit h?chster Wahrscheinlichkeit h?matogenen Ursprungs.
en-copyright=
kn-copyright=

en-aut-name=ShindohNaosaku
en-aut-sei=Shindoh
en-aut-mei=Naosaku
kn-aut-name=進藤直作
kn-aut-sei=進藤
kn-aut-mei=直作
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學柿沼内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ウシおよびブタ好中球の同種精子に対する体外での走化性および貪食能に影響する要因について
kn-title=Factors affecting the chemotactic and phagocytotic activities of bovine and porcine neutrophils for the homogenous sperm in vitro
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=李井春
kn-aut-sei=李
kn-aut-mei=井春
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END

start-ver=1.4
cd-journal=joma
no-vol=42
cd-vols=
no-issue=9
article-no=
start-page=2099
end-page=2107
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1930
dt-pub=19300930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=?ber die histologischen Ver?nderungen des Knochenmarks nach der Splenektomie
kn-title=剔脾後ニ於ケル骨髓ノ組織學的變化ニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Verfasser beobachtete die histologischen Ver?nderungen des Knochenmarks des Os femoris. zeitlich, d. h. am 7. und 30. Tage nach der Splenektomie. Als Versuchstiere verwandte er, unter m?glichst gleichen Bedingungen, gleichzeitig entbundene jugendliche Hunde. Die Ergebnisse der Versuche sind folgende: 1) Die Blutkapillaren im Knochenmark zeigen deutliche Dilatation und merkliche Hyper?mie einen Monat nach der Splenektomie. 2) Die Erythroblasten, Lymphocyten, und Knochenmarksriesenzellen haben schon eine Woche nach der Splenektomie die Neigung sich zu vermehren, dann nehmen sie innerhalb eines Monates nach der Splenektomie merklich zu. Die Eosinophilen Leukocyten haben auch die Neigung sich zu vermehren, neutrophile Leukocyton jedoch nehmen allm?hlich ab und sind nach einem Monat nicht mehr halb so zahlreich als vor der Operation. 3) Er beobachtete den Zustand des Retikuloendothelsystems bei Vitalf?rbung mit Karmin. Retikuloendothelzellen haben schon eine Woche nach der Splenektomie die Neigung zu hypertrophieren. Nach dem Verlauf eines Monates zeigen sie auffallende Hypertorphie und Hyperplasie, und speichern in ihrem Leibe zahlreiche Karmink?rner. 4) Die Retikuloendothelzellen im Knochenmark nehmen mit dem Alter allm?hlich ab. 5) Die Eisenreaktion (nach Perls-Stiedas) im Knochenmark kommt schon eine Woche nach der Splenektomie deutlich zum Vorschein, und in den Retikulumzellen und der Blutkappilarenwand werden zahlreiche feine K?rner, welche sich gegen Eisenreaktion positiv zeigen, sicht bar. Doch einen Monat nach der Splenektomie kehrt der Grad der Eisenreaktion wieder auf den normalen Zustand zur?ck. 6) Die oben erw?hnten histologischen Ver?nderungen im Knochenmark nach der Splenektomie erscheinen deutlich in der Jugend als im Alter.
en-copyright=
kn-copyright=

en-aut-name=WatanabeDenji
en-aut-sei=Watanabe
en-aut-mei=Denji
kn-aut-name=渡邊傳二
kn-aut-sei=渡邊
kn-aut-mei=傳二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學泉外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=49
cd-vols=
no-issue=4
article-no=
start-page=769
end-page=781
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1937
dt-pub=19370430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Recherches exp?rimentales sur la r?action inflammatoire. III. Sur la formation des bulles par l'emplatre de cantharidine et sur les modification histologiques qui en r?sultent.
kn-title=炎衝反應傾向ニ就テ（其ノ3）「カンタサヂン」發疱ト疱内細胞ノ變化
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Nous avons publi? dans nos Pr?c?dents travaux nos observations sur la formations des bulles par l'empl?tre de cantharidine et sur les modifications du PH et du taux du sucre dans le liquide des bulles aussi bien que dans le sang, soit apr?s blocage de l'appareil r?ticulo-endoth?lial, soit apr?s injection de substances "tampons" (pbosphate acide ou alcalin), soil enfin apr?s injection de cas?osane aux lapins en exp?rience. Dans le cas pr?sent, nous avons fait quelques remarques histologiques sur lesglobules blancs renfermes par le liquide des bulles au cours de l'exp?rience. Les r?sultats obtenus sont les suivants. 1) Chez les lapins normaux, le nombre des globules blancs du liquide des bulles est, au d?but, presque le m?me que dans le sang. Avec le temps, il augmeute jusqu'? 20.000. I1 est constitu? ? peu pr?s uniquement par des leucocytes neutrophiles, parmi lesquels se trouvent environ 2% de cellules lympho-histiocytaires. 2) Chez les lapins qui ont recu de la cas?osane en injection, ? la dose quotiiiienne de 0, 01cc par Kilogramme de poids d'animal pendant 10 jours cons?cntifs, nous avons constat? que le nombre des leucocytes ?tait promptement augment? et que les cellules lymphohistiocytaires ?taient les plus abondantes. 3) Chez les lapins auxquels nous avons inject? de la cas?osane une seule fois ? la dose de 0, 2cc par Kg. de voids d'animal, les modifications histologiques ?taient ? peu pr?s les m?mos que cellos que nous avons observ? chez les lapins ayant recu des injections consecutiv?s de cas?osane. 4) Chez les lapins ayant subi une saignee de 6cc par Kg. de poids d'animal, on a constat? que la formation des bulles ?tait plus nette et que le nombre total des leucocytes s'accroissait plus rapidement.
en-copyright=
kn-copyright=

en-aut-name=Kam?yamaS.
en-aut-sei=Kam?yama
en-aut-mei=S.
kn-aut-name=亀山茂松
kn-aut-sei=亀山
kn-aut-mei=茂松
aut-affil-num=1
ORCID=

en-aut-name=ItanoS.
en-aut-sei=Itano
en-aut-mei=S.
kn-aut-name=板野坂惠
kn-aut-sei=板野
kn-aut-mei=坂惠
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學柿沼内料教室

affil-num=2
en-affil=
kn-affil=岡山醫科大學柿沼内料教室
END

start-ver=1.4
cd-journal=joma
no-vol=51
cd-vols=
no-issue=7
article-no=
start-page=1583
end-page=1604
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1939
dt-pub=19390731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=?ber 2 F?lle von aleukaemischer Lymphadenose
kn-title=Aleukaemische Lymphadenoseノ2剖檢例
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Erster Fall: Ein 57-j?hriger heruntergekommener Kaufmann wurde am 27.11. 1936. in unsere Klinik aufgenommen. Status praesens: Statur mittelgross, Koerperbau m?ssig, Ern?hrung sehr schlecht, Haut und Schleimhaut deutlich an?misch, Lippe und Zunge mehr trocken, rechtseicht aufgetrieben, Ascitesfl?ssigkeit nicht vorhanden, Leber in der Mamillalinie 3 querfingerbreit unterhalb des Rippenbogens derb f?hlbar. Aufallendste Befunde waren Lymphdr?senanschwellungen. Submaxillare, cervicale, axillare und inguinale Lymphdr?sen waren von Taubenei- bis uber Huhnereigr?sse angeschwollen, elastisch derb, gut beweglich, nicht so druckempfindlich, sowohl mit der Haut, als auch miteinander nicht verwachsen. Wassermann negativ. Urin normal. Leukozyten 9.200, H?moglobin 22%, Erythrozyten 3, 420, 000. Das Blutbild ergibt basophile Leukozyten 2%, Eosinophile L. 0%, Stabkernige L. 1%, Segmentkernige L 43%, Lymphozyten 48%, gr?sse mononukle?re Zellen und ?bergangsform 5%. Beobachtenswert ist die sehr geringe Vermehrung in der Gesamtzahl der Leukozyten. Eine Lymphdr?se wurde aus der Achselhohle exstirpiert und histologisch untersucht und nur eine Lymphozyteninfiltration konstatiert. Die Lymphknoten waren durch Rontgentherapie sehr verkleinert worden. Der Patient verliess am 12.12. das Krankenhaus, um am 11. 1. 1937. wieder zur?ckkehren. Seit der Entlassung hatte er wieder Lymphdr?senanschwellung und hochgradige Schw?che. Exitus letalis am 2. 2. 1937. Bei der Sektion einwandfrei lymphatische, leukaemische Infiltration sowohl in den blutbildenden Organen, wie auch in fast allen anderen Geweben nachgewiesen. Die lymphatische Zellen hatten zweifellos auch das Knochenmark ergriffen, die erythroblastischen Zentren in Mitleidenschaft gezogen und hatten zu einer Blutarmut gef?hrt. 2ter Fall: Ein 23-j?hriger Bauer kam am 20. 9. 1937. in einen das Krankenhaus. Vor 5 Monaten bemerkt er am r-Seitenhals einen erbsengrossen Tumor, der sich allm?hlich vergr?ssert und vermehrt, aber beschwerdelos verlauft. Seit Monaten mit der Zeit cervicale (1-), submaxillare und inguinale Lymphdrusen. Diese waren von Taubenei-bis ?ber H?hnereigrosse angeschwollen nebeneinander, elastisch derb, nicht so druckempfindlich, mit der Unterlage fixiert, aber mit der Haut nicht verwachsen. Keine Atmungs-und Schluckbeschwerde. Appetit normal. Fieber 37-37.5°C. Urin normal. WaR. negativ. Blutsenkungsgeschwindigkeit 5.5 (1st), 15 (2st). Leukozyten 4.4000, Hamoglobin 90%, Erythrozyten 518, 000. Das Blutbild ergibt Lymphozyten 42%, neutrophile L. 43%, stabkernige L. 5%, Monozyten und ?bergangsform 9.5%, keine jugendlichforme Leukozyten. Eine Lymphdr?se wurde aus dem Seitenhals exstirpiert und histologisch untersucht und nur eine Lymphozyteninfiltration konstatiert. Die Untersuchung erwiess aleukaemische Lymphadenose. Nach 7 Monaten exitus letalis. Im Vordergrund der heutigen Therapie steht die Strahlentherapie, vor allem Rontgenbehandlung. Wichtig und erfolgreich ist die Arsen-Behandlung. Sie wird angewandt, wenn die Arsentherapie erfolglos und der Allgemeinzustand und das ganze Krankheitsbild einer Behandlung bed?rftig ist. Die Rontgenbestrahlung richtet sich hier vor allem gegen die peripheren Lymphdr?senpakete, aber auch gegen die Milz, selbst wenn sie nicht wesentlich vergr?ssert ist, weil oftmals erst nach der Milzbestrahlung die Leukozytenzahl zur Norm absinkt, obwohl die Lymphdr?sen vorher schon zuruckgingen. Mit 30% H.E.D. auf jedes Feld, wobei man 1 Feld pro Tag vornimmt und die Leukozytenzahl t?glich kontrolliert. Die Intensivbestrahlung, die einen plotlichen tiefen Absturz bringt, ist vollig unzweckm?ssig, wie auch jede nicht genau kontrollierte Strahlentherapie. Sie kann direkt sch?dlich seiu dadurch, dass die Leukozytenzahl schnell unter die Norm sinkt und diesem starkem Abfall rasch wieder eine Verschlimmerung und ein h?her Anstieg mit Komplikation des roten Blutbildes usw. folgt (Reizwirkung). Die Rontgenbehandlung wird wiederholt, wenn das Krankheitsbild sich wieder verschlechert. Sie hat aber dann m?glichst bald einzusetzen und nicht erst bei weitgehender Verschlimmerung. Der Kranke muss also in dauernder Beobachtung bleiben und alle 3-4 Wochen soll ein morphologischer Blutstatus aufgenommen werden. In der Zeit zwischen den eiuzelnen R?ntgenbestrahlungen nimmt man zweckm?ssigerweise Arsentherapie vor. Rezidive treten immer auf. Die Leukaemie ist durch kein Mittel zu heilen. Sie wird vielmehr ?ber kurz oder lang gegen jede Behandlung auch die Strahlenbehandlung refrakt?r und f?hrt schliesslich unter dem Bild einer akuten Verschlechterung oder durch Komplikationen zum Tode. Die R?ntgentherapie muss abgebrochen werden, wenn die Zahl der weissen Blutzellen sehr schnell und tief absinkt, wenn zahlreiche unreife Zellen auftreten, wenn das rote Blutbild aber das Allgemeinbefinden sich unter der Bestrahlung akut verschlechtert, und Gewichtssturz, Durchf?lle und h?heres Fieber eintreten. Ber vorsichtiger Dosierung und dauernder strenger Kontrolle jeder einzelnen Bestrahlung treten aber derartige Verschlimmerungen erst nach evtl.langj?hriger Bestrahlung auf.
en-copyright=
kn-copyright=

en-aut-name=KuwabaraSei
en-aut-sei=Kuwabara
en-aut-mei=Sei
kn-aut-name=桑原正
kn-aut-sei=桑原
kn-aut-mei=正
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學津田外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=52
cd-vols=
no-issue=2
article-no=
start-page=458
end-page=466
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1940
dt-pub=19400229
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=?ber einen Sektionsfall von symptomatischer Agranulozytose
kn-title=剖檢セル症候性「アグラヌロチトーゼ」ノ1例
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Verfasser berichten ?ber einen Sektionsfall von symptomatischer Agranulozytose, die nach der antiluetischer (Salvarsan) Behandelung auftrat. Es handelt sich um einen 30-jahrigen Mann, der fruher Lues und Tripper durchgemacht hatte. Das Blutbild bei der ersten Untersuchung war folgendes: H?moglobingehalt (nach Sahli) 28%, Erythrozytenzahl 1360, 000, Leukozytenzahl 1, 800 (Neutrophile polymorphkernige Leukozyten 11.5%, Lymphozyten 82.5%, Eosinophile polymorphkernige Leukozyten 1.0%, grosse Mononuklearen und ?bergangsform 2.0%, Plasmazellen 3.0%). Der Patient starb am zehnten Tage nach der ersten Untersuchung; die Leiche wurde sofort seziert. Die haupts?chlichsten Sektionsbefunde waren eine deutliche nekrotische Entz?ndung der beiden Tonsillen und das an Femur und Sternum gefundene Fettmark. Gegen das Ende wurde Leukozytose (9, 800) festgestellt, die die Verfasser dahin deuteten, dass sie durch sekund?re Infektion hervorrufen wurde. Bei der Pathogenese legten sie grossen Wert auf die Anlage oder die Disposition.
en-copyright=
kn-copyright=

en-aut-name=NakataFujio
en-aut-sei=Nakata
en-aut-mei=Fujio
kn-aut-name=中田富士男
kn-aut-sei=中田
kn-aut-mei=富士男
aut-affil-num=1
ORCID=

en-aut-name=KimuraTsutomu
en-aut-sei=Kimura
en-aut-mei=Tsutomu
kn-aut-name=木村勉
kn-aut-sei=木村
kn-aut-mei=勉
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學柿沼，北山内科教室

affil-num=2
en-affil=
kn-affil=岡山醫科大學柿沼，北山内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=53
cd-vols=
no-issue=6
article-no=
start-page=1281
end-page=1299
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1941
dt-pub=19410630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=?ber 7 F?lle von maligner Lymphogranulomatose
kn-title=惡性淋巴肉芽腫7例
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Der Verf. hat an 7 F?llen von maligner Lymphogranulomatose, die er in der letzten Zeit betroffen hatte, klinische Beobachtungen angestellt. Unter den F?llen fand sich ein verh?ltnism?ssig seltener Fall von Abduzensl?hmung und Facialisl?hmung. Die Geschlechter waren so beteiligt, dass sich 3 F?lle bei M?nnern und 4 F?lle bei Frauen fanden. Das Alter der Patienten lag zwischen 17 und 66 Jahren. Nach dem Beruf waren 5 Pat. Bauern und 2 Pat. Kaufleute. Die prim?re Auftretungsstelle der Krankheit lag im Hals. Temperatursteigerungen zeigten 6 F?lle, in denen 4 F?lle unter Febris continua, 2 F?lle unter Febris intermittens litten. Die Zahl der roten Blutk?rperchen schwankte zwischen 2, 66 und 5, 57 Millionen. Die Lymphozytenzah. war in 5 F?llen normal. Leukozytose, die die weissen Blutk?rperchen mehr als 10 Tausend betrug, befand sich in 2 F?llen. Neutrophile Polymorphkernige Leukozytose wurde in 6 F?llen bepbachtet. Lymphopenie wurde in allen 7 F?llen festgestellt. Als die beste Therapie empfielt der Verf., wenn es sich um verh?ltnism?ssig fr?here Stadien handelte, operative Exstirpation der Krankheitsherde und R?ntgenbestrahlung. Auch f?r fortgeschrittene Erkrankung kann man nach dem Erachten dee Verf. s nicht selten durch Anwendung der R?ntgenbeatrahlung sicher recht g?natige Erfolge erzielen.
en-copyright=
kn-copyright=

en-aut-name=YamamotoEikichi
en-aut-sei=Yamamoto
en-aut-mei=Eikichi
kn-aut-name=山本英吉
kn-aut-sei=山本
kn-aut-mei=英吉
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學石山外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=2
article-no=
start-page=171
end-page=193
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1944
dt-pub=19440229
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studien uber Dengue I. ?ber das Bild des Blutes und des Knochenmarks bei Dengue
kn-title=「デング熱」ニ關スル研究（第1編）血液像及ピ骨髓像ニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bez?glich des Blutbildes bei Dengue verf?gt die Literatur ?ber zahlreiche Beobachtungen, welche jedoch in den Ergebnissen etwas verschieden lauten und somit nicht wenig freies Feld f?r weitere Untersuchungen noch offen lassen. Was aber das Bild des Knochenmarks bei Dengue anbetrifft, so findet der Verf. in der Literatur keinen Fall ver?ffentlicht. Bei der Infektion des Denguefiebers, welche im letzten Sommer in der Stadt Nagasaki stattfand, hat der Verf. in 37 F?llen den Verlauf der Krankheit t?glich verfolgt, dabei die Verschiebungen des Blutbildes beobachtet und in anderen 10 Fallen das Bild des Knochenmarks untersucht. Dadurch hat er folgende Ergebnisse erhalten. 1. Bei den einzelnen Erythrozyten werden weder im peripheren Blut noch im Knochenmark erhebliche Variationen beobachtet. 2. Die Zahl der Leukozyten erf?hrt von Anfang der Krankheit an eine Abname, welche vom 2. Krankheitstage in erheblichem Masse auft itt und im 4. und 5. Tage das Maximum erreicht, um nach Ablauf dieser Tage rasch zur Norm zur?ckzukommen. 3. Neutrophile Leuk?zyten nehmen nach Ablauf des 1. Tages, wo sie eine geringe Zunahme zeigen, in starkem Masse ab und kommen nur langsam zur Norm zuruck. Bei ihnen besteht die Linksverschiebung der Kerne, auch die sog. Vergiftungsgranula und die Degeneration der Vakuolen sind in der Regel hochgradig aufgetreten. Es wurde auch beider Gruppe der neutrophilen Leukozyten des Knochenmarks eine auffallend betrachtliche Reifungsst?rung festgestellt, welche wahrscheinlich darin begr?nden l?sst, dass trotz der hochgradigen Degeneration der Vakuolen in denjungen Knochenmarkszellen die alteren Zellen des Knochenmarks keine quantitative Abnahme erleiden, die w?hrend segmentierten neutrophilen Leukozyten eine erhebliche Abnahme erfahren. Nach dem Erachten des Verfassers soll die Verringerung der neutrophilen Leukozyten im peripheren Blut gewiss einer afferenten und einer efferenten Natur sein. 4. Eosinophile Leukozyten vermehren sich allm?hlich mit der kleinen Ausnahme, dass sie nur im Verlauf des 2. Tages verschwinden. Es liegt aber die Annahme nahe, dass bei den Eosinophilen keine Leukozytose nach der Infektion stattfindet. 5. Die grossen Mononuclearen nehmen im Fr?hstadium relativ etwas zu, um nach Ablauf diesen Stadiums eine absolute Zunahme zu zeigen, welche 2-3 Wochen nach der Abfieberung noch fortschreitet, w?hrend im Knochenmark keine Zunahme der betr. Zellen beobachtet wird. 6. Lymphozyten nehmen schon von dem 1. Tag an betr?chtlich ab, nach der Entfieberung aber tritt postinfekti?se Lymphozytose in rascher Folge auf. 7. Eine ?berm?ssige Vermehrung der Plasmazellen, welche transitorisch im Stadium des mit Schwellung der Lymphdruse einhergehenden Exanthems in besonders hohem Masse stattfindet, ist in 38,25-3,5% der gesamten F?lle beobachtet. 8. Es wurde festgestellt, dass die Blutpl?ttchen um das Stadium des Exanthems abnehmen.
en-copyright=
kn-copyright=

en-aut-name=TakayamaYoshitake
en-aut-sei=Takayama
en-aut-mei=Yoshitake
kn-aut-name=高山義武
kn-aut-sei=高山
kn-aut-mei=義武
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=陸軍軍醫學校軍陣内科學教室
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=11
article-no=
start-page=2157
end-page=2166
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19561130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Sympathic Control of the Bone Marrow Part III The change of peripheral blood picture after the lumbal sympathectomy
kn-title=骨髓の神経性調節に関する研究 第三編 腰部交感神経幹切除後の末梢血液像の変化
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The change of periphral blood picture was relalively examined after the lumbal sympathectomy and even operation by no sympathectomy of dogs, and the results obtained were as follows: 1) No shortened the recovery from the decrease of hemoglobine and erythrocyte count. 2) No reduced the decrease of hemoglobin, but reduced clearly decrease of erythrocyte count. 3) Inconstant about leukocyte count. 4) Inconstant about staff neutrophils, but reduced the increase of total neutrophils. The above concludes that the interception of sympathic nerve of dogs promotes the increase of erythrocytes and represses the increase of neutrophils in peripheral blood.
en-copyright=
kn-copyright=

en-aut-name=ShibataTamotsu
en-aut-sei=Shibata
en-aut-mei=Tamotsu
kn-aut-name=柴田完
kn-aut-sei=柴田
kn-aut-mei=完
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=11
article-no=
start-page=2117
end-page=2139
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19561130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Sympathic Control of the Bone Marrow Part 1 The change of femur bone marrow picture after the lumbal sympathectomy
kn-title=骨髓の神経性調節に関する研究 第一編 腰部交感神経幹切除後の大腿骨々髄像の変化
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The change of femur bone marrow picture was examined after the lumbal sympathectomy of rabbits and dogs, and the results obtained were as follows: 1) No changes by macroscopic finding. 2) No changes of nucreated cell count. 3) Relatively increasing of erythroblasts, and decreasing of pseudoeosinphils (rabbits) or neutrophils (dogs), but those mitosis count both increased. 4) Erythroblasts, pseudoeosinophils (rabbits) and neutrophils (dogs) all indicated marrowright-shift, in other words, the promotion of the maturity. 5) Staff and lobed pseudoeosinophils (rabbits) or neutrophils (dogs) indicated decrease, in other words, the promotion of cell outswim or cell outflow. The above concludes that the intercreption of sympathic nerves of rabbits and dogs promotes mainly medullary cell outflow and maturiy.
en-copyright=
kn-copyright=

en-aut-name=ShibataTamotsu
en-aut-sei=Shibata
en-aut-mei=Tamotsu
kn-aut-name=柴田完
kn-aut-sei=柴田
kn-aut-mei=完
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=12
article-no=
start-page=3181
end-page=3198
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19571231
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A clinical study of the general anesthesia for the patients with anemia and hypoproteinemia Part 1. The changes of the blood components before and after cyclopropane anesthesia in anemic and hypoproteinemic patients
kn-title=貧血ならびに低蛋白血症患者の全身麻酔に関する臨床的研究 第1編 貧血および低蛋白血症患者の全身麻酔前後における血液諸成分の変動について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Thirty-three patients with anemia and hypoproteinemia were anesthetized with cyclopropane and the changes in the peripheral blood corpuscles and the plasma protein were observed before and after anesthesia. 1) The number of the erythrocytes slightly increased already 20 minutes after starting anesthesia, while the leucocytes markedly increased. In the increase of the leucocytes, An increase of the neutrophils and an marked increase of the lymphocyte were observed. Hemoglobin as well as hematcrit increased, while the water content of blood and plasma decreased. 2) Total plasma protein increased and its fractioning revealed an increase of A/G ratio, slight increase of albumin, decrease of α-globulin as well as φ, slight increase of γ-grobulin and no change of β-globulin in percentage. From the quantitative point, there were observed an increase of Al. slight increase of β-Gl., decrease of φ, increase of γ-Gl. and no change of α-Gl.. 3) These changes in the peripheral blood corpuscles and the plasma protein are considered to be due to the temporary increase of adrenalin secretion caused by cyclopropane as stressor, though there may be some exemia (concentration of the blood) due to general anesthesia.
en-copyright=
kn-copyright=

en-aut-name=KunitomoKeiichi
en-aut-sei=Kunitomo
en-aut-mei=Keiichi
kn-aut-name=国友桂一
kn-aut-sei=国友
kn-aut-mei=桂一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=8
article-no=
start-page=2175
end-page=2190
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570830
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Sympathetic Interception on the Bone Marrow Part. I. Cultures in Coverships of Femur Bone Marrow after the unilateral lumbar Sympathectomy
kn-title=交感神経遮断の骨髓に及ぼす影響 第1編 骨髄被覆培養法に依る研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cultures in coverslips of femur bone marrows of unilateral sympathectomized rabbits were compaired with each other side on the following 3, 5, 10, 15, 20, 30 and 90 days. The results obtained were as follows: (1) The wandering velocity of neutrophils of bone marrow of the sympathectomized side is larger than the another side, especially on the 5 th day after the operation. (2) The relative growth rate is larger than the other non-operated side on the 5 th and 10 th days after the operation. (3) The cell dencity is strikingly larger in the sympathectomized side on its 3rd, 5th, 10 th and 30th day groups after the operation. (4) Each figures show the maximum on about 5th day group after the operation, and after 30 days the results did not appear to differ between both sides, and these effects are transitory. Thus the interception of the sympathetic in rabbits promotes the function of the bone marrow transitorily. In other words the sympathetic reduces the function of the bone marrow.
en-copyright=
kn-copyright=

en-aut-name=NagaseMasaki
en-aut-sei=Nagase
en-aut-mei=Masaki
kn-aut-name=永瀬正己
kn-aut-sei=永瀬
kn-aut-mei=正己
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=8
article-no=
start-page=2049
end-page=2072
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570830
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Influences Exerted on the Bone Marrow by the Blocking of Parasympathetic Nerves Part 1 Influences on the Picture of the Bone Marrow Obtained from the Lower Extremeties by Section of Lumbosacral Posterior Root of Spinal Cord of Dog
kn-title=副交感神経遮断の骨髄に及ぼす影響に関する研究 第一編 犬腰仙部脊髄後根切除の下肢骨髄像に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By cutting off the posterior roots of the fifth, sixth and seventh lumbar regions of spinal cord as well as of the first sacral segment, on the left side of young dog, the author studied the changes in the bone marrow picture of the lower extremeties comparing that on the right side as the control, and obtained the following results: 1) No marked change can be recognized in macroscopic findings of the bone marrow in the femur and tibia. 2) In the bone marrow of the femur there is a tendency of a slight decrease in the number of nucleated cells as compared with that on the side of the control. 3) In the bone marrow of the tibia, though no difference can be recognized between erythrocytes and neutrophils, in the femur bone marrow erythrocytes have relatively decreased but neutrophils have increased as compared with those of the control. 4) There is a tendency of a decrease in the mitoses of erythrocytes and neutrophils as compared with those on the side of the control. 5) Both erythrocytes and neutrophils as compared with those on the side of the control present a picture of a shift to the left, namely, an inhibition of maturation. 6) Segmented neutrophils have increased in number as compared with the control, indicating an inhibition of the cell outflow from the bone marrow. From these the author arrived at the conclusion that the blocking of parasympathetic nerves primarily inhibits the cell outflow from the bone marrow and accompanying this, cell maturation is also inhibited in the parenchyma of the bone marrow.
en-copyright=
kn-copyright=

en-aut-name=IshidaShusaku
en-aut-sei=Ishida
en-aut-mei=Shusaku
kn-aut-name=石田收作
kn-aut-sei=石田
kn-aut-mei=收作
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=6
article-no=
start-page=1635
end-page=1651
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Oxygen Consumption and Glycolysis of Bone Marrow Tissue Part IV On the O(2)-Consumption and Glycolytic Metabolism of Essential Chloranemias Bone Marrow
kn-title=骨髓の組織呼吸並に解糖作用に関する研究 第4編 本態性萎黄貧血骨髄の呼吸解糖代謝に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=At first with sternum-puncture of the patients suffering from essential chloranemias (idiophathic hypochlomic anemia), the observations on the bone-marrow picture and the determinations of the O(2)-consumption and glycolysis had been conducted; and then, after adding the serum of the patient to the bone marrow of normal rabbit, in vitro, again the rates of the O(2)-consumption as well as of glycolysis of the bone marrow of the rabbits receiving continuously for one week injections of the same serum were determined. With these results and with comparative studies on the case of bleeding anemia with that of hook-worm anemia, the author have drawn the following conclusions: 1. The number of nucleated cells in the punctuate of the essential-chlorosis patient as well as the percentage of the erythroblasts in the bone-marrow picture increased; and the majority of the latter were basophilic; and furthermore, of those that were neutrophilic, promyelocytes and or metamyelocytes showed a slight increase in number while mature cells revealed a decrease. 2. The O(2)-consumption and glucolysis of the punctuate drawn from the patient showed an increase in the rate, the latter especially markedly; whereas the fat-free dry weight of the punctuate had been light but after the treatment it showed a tendency to return to normal. 3. On comapring the O(2) consumption and glycolysis of the bone marrow of the rabbits receiving continuous injections of the patient's serum with those of the bone marrow of the rabbits injected with the serum of the normal, no marked difference whatever could be recognized. 4. Similar comparative observations made with regard to the effects on the O(2)-consumption both in the case where the patient's serum had been added to the normal rabbit bone-marrow and where the normal person's serum to the rabbit's bone marrow, revealed no substantial difference. 5. A marked rise of the rates of both the O(2)-consumption and glycolysis of the punctuate from the patient is in a strange contrast to the case of hemorrhagic anemia where there is no change at all; and furthermore, the rise has a certain similarity to the rising picture of hookworm anemia but the degree of the rise in the case of this desease is far greater than that of the latter.
en-copyright=
kn-copyright=

en-aut-name=IshibashiTadao
en-aut-sei=Ishibashi
en-aut-mei=Tadao
kn-aut-name=石橋忠男
kn-aut-sei=石橋
kn-aut-mei=忠男
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=2
article-no=
start-page=447
end-page=460
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on effects of high hydrostatic pressure on blood cells Part I. On the phagocytosis of leucocytes
kn-title=高圧の血球に及ぼす影響に関する研究 第一編 白血球の貪喰能に関する高圧の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Experiments have been performed to examine the phagocytosis of human blood leucocytes on which high hydrostatic pressure has been applied.
The results were as follows: 1) At 50-100 atm. pressure, there was no difference in phagocytes number against control for 30-60 min., but increase for 120 min. 2) At 200-2000 atm. pressure, the difference in phagocytes number was found as compared with control for 30-120 min. The higher the pressure was, the less phagocytes was. 3) The rate of phagocytosis average degree showed a tendency to be same with the rate of positive degree of phagocytosis. 4) At one atmospheric pressure, monocytes have the most powerful phagocytic activity, then come neutrophilic, eosinophilic leucocytes and lymphocyte in the order of their phagocytic activity. Especially, lymphocytes show no fluctuation on the rate of positive degree of phagocytosis for almost any time under pressure. Phagocytosis of lymphocytes has not been effected by pressure. 5) Phagocytic activity of monocytes and neutrophilic leucocytes have been influenced by pressure, but eosinophilic leucocytes and lymphocytes less than the two formers. 6) It was discussed whether the effect of high hydrostatic pressure on phagocytosis of leucocytes is caused by the alternation of the surface tention between leucocyte and its surrounding medium, and the streaming of leucocytes protoplasma. 7) For the soap solution-petroleum system, the interfacial tention decreases under pressure (0-500 atm.).
en-copyright=
kn-copyright=

en-aut-name=MiyatakeTaka'aki
en-aut-sei=Miyatake
en-aut-mei=Taka'aki
kn-aut-name=宮武孝明
kn-aut-sei=宮武
kn-aut-mei=孝明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一生理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=10-1
article-no=
start-page=6293
end-page=6309
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Leukemia Part 2. Clinical and Statistical Observations of the Peripheral Blood Picture in Various Leukemias
kn-title=白血病に関する研究 第2編 各種白血病に於ける末梢血液像に関する臨床統計的観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author carried out clinical and statistical observations of the peripheral blood picture in various leukemias occurring in the Chugoku-Shikoku District, with a special reference to the comparative study on the two groups treated in our department, namely, the group whose bone marrow was cultured and the other without such a culture finding. However, cases of chronic lymphocytic leukemia are not included in the present statistics as their number was trivial. 1. Hb content and erythrocyte count: The decrease in Hb content and erythrocyte count is most marked in acute myelogenous leukemia, followed by acute lymphocytic leukemia, and it is least in chronic myelogenous leukemia. The decrease in Hb and erythrocytes of monocytic leukemia occupies an intermediate position between the above two. 2. The color index: The color index is comparatively high in acute type while it tends to be lower in chronic type. 3. Leucocyte count: The aleukemic form is greatest in monocytic leukemia followed in descending order of that in acute lymphocytic leukemia and acute myelogenous leukemia, and all of these aleukemic ones occupy more than 1/3 of the total. However, in chronic myelogenous leukemia it is extremely rare to find the aleukemic form. 4. Reticulocyte count: The reticulocyte count in the peripheral blood of leukemia is generally normal or is increased. 5. Platelet count: The platelet count is about at the normal level in chronic myelogenous leukemia, and in some it is increased. However, it is decreased in all other leukemias. In monocytic leukemia it resembles that in acute type. 6. Classification of leucocytes: In acute myelogenous leukemia myeloblasts occupy a greater proportion and also matured leucocytes are quite many. Intermediate immature cells are low in percentage but it is rare not to see any intermediate immature cells. In chronic myelogenous leukemia mature neutrophils are numerous but immature neutrophils are a few in number. In acute lymphocytic leukemia a high percentage of lymphatic cells accompanied by a marked increase in lymphoblasts can be observed.
In monocytic leukemia intermdiate mature cells are in a low proportion, and monoblasts are less than in acute type, presenting the characteristic intermediate between acute type and chronic type. Namely, monocytic leukemia is an intermediate type between acute leukemia and chronic leukemia, and it is difficult to divide it into acute and chronic types. 7. Relationship between the leucocyte count and leucocyte percentage: On the whole the increase in the number of leucocytes and myeloblast percentage show a mutual relationship. In monocytic leukemia those whose increase in the number of leucocytes is more marked show a greater number of monoblasts, approaching to the acute type. 8. Relationship between the leucocyte count and platelet count: There can be seen no relationship between the leucocyte count and platelet count. 9. Relationship between the leucocyte count and swelling of the spleen and lymph nodes: In acute lymphocytic leukemia the more marked is the increase in the number of leucocytes the greater is the palpitation frequency and the degere of swelling of the spleen and lymph nodes. In chronic myelogenous leukemia the degree of splenomegaly increases along with the increase in the nmnber of leucocytes.
en-copyright=
kn-copyright=

en-aut-name=MatsuyamaTsuneo
en-aut-sei=Matsuyama
en-aut-mei=Tsuneo
kn-aut-name=松山恒男
kn-aut-sei=松山
kn-aut-mei=恒男
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=9-2
article-no=
start-page=5955
end-page=5976
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590910
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Bone-Marrow Tissue Culture Part 1. On the Course of Bone-Marrow Tissue Culture
kn-title=骨髄体外組織培養に関する研究 第1編 骨髄体外組織培養の経過について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By performing tissue culture of the bone-marrow aspirated from the sternum of normal persons, ribs removed at the thoracoplastic surgery, and from the femur of normal rabbit, the author observed the course of the bone-marrow tissue culture, and obtained the following results. 1. In the bone-marrow tissue culture of the human sternum the growth area keeps on increasing up to 18 hours of the culture and its average relative growth rate is 10.64; in the case of the rib removed at thoracoplastic surgery it lasts for 36 hours and the average of its relative growth rate is 16.90; and in the case of the rabbit femur it lasts for 48 hours and the average of its relative growth rate is 45.37. The fluctuation curve of the average relative growth rate in each case is somewhat like a parabola. 2. The average of the cell density index in the case of human sternum is 52 at 18 hours after the start of calture; in the case of the rib removed at the thoracoplastic surgery it is 54 after the 24-hour culture; and in the case of the rabbit femur it is 67 after the 24-hour culture. 3. In the case of the sternum of normal persons the average value of wandering velocity of neutrophils reaches the maximum of 15.24μ/m three hours after the start of the culture and neutrophils cease wandering on the fourth-sixth day of the culture; in the case of the rib removed at the thoracoplastic surgery the average of the wandering velocity reaches the maximum of 10.51μ/m, and the wandering of neutrophils ceases in the sixth to seventh day of the culture; and in the case of normal rabbit femur the average wandering velocity of pseudoeosinophils reaches the maximum of 14.17μ/m immediately after the start of the culture and the wandering of pseudoeosinophils stops on the 5th to 6th day of the culture. 4. The cell growth area is divided into the central, intermediate and peripheral zones. The course of cell activity in the growth area is divided into the initial, middle, and terminal stages, and obsewations have been carried on from stage to stage.
en-copyright=
kn-copyright=

en-aut-name=WatariZenji
en-aut-sei=Watari
en-aut-mei=Zenji
kn-aut-name=亘理善治
kn-aut-sei=亘理
kn-aut-mei=善治
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=9-1
article-no=
start-page=5607
end-page=5615
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590830
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Cancerous Toxic Substance with Bone-Marrow Tissue Culture Part 3. A study on the tissue culture of the bone marrow aspirated from sternum of cancer patients
kn-title=骨髄組織培養法を応用せる癌毒性物質に就ての研究 第三編 癌患者胸骨々髄体外組織培養に就ての研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=On the basis of the results obtained in Parts 1 and 2 the author studied the erythropoietic and leucopoietic functions in cancer patients by means of bone-marrow tissue culture and also observed the wandering velocity, vital staining and carbon-particle phagocytosis of neutrophils appearing in the growth area; and obtained the following results: 1. The tissue growth in cancer patients is diminished fiarly markedly as compared with that in normal persons, the control. 2. Although there are cases showing the wandering velocity of neutrophils almost at the normal level, in more than one half of them the wandering velocity seems to be slightly lower than that in the control. 3. By the vital staining of neutrophils the function of the cells is likewise diminished as compared with the control. 4. The carbon-particle phagocytotic ability of neutrophils is also diminished when compared with the control. 5. By the bone-marrow tissue culture in a fluid medium it has been observed that on the whole erythropoiesis tends to show a slight decrease while Hb content a slight increase as copared with the control. 6. From these the leucopoietic function of the bone marrow in various cancer patients is somewhat diminished, and also judging from the wandering ability, finding of vital staining and carbon-particl phagocytosis, function of neutrophil is similarly decreased, but the erythropoietic function of the bone marrow can not necessarily be judged as to have declined.
en-copyright=
kn-copyright=

en-aut-name=TanakaToshio
en-aut-sei=Tanaka
en-aut-mei=Toshio
kn-aut-name=田仲俊雄
kn-aut-sei=田仲
kn-aut-mei=俊雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=9-1
article-no=
start-page=5587
end-page=5596
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590830
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Cancerous Toxic Substance with Bone-Marrow Tissue Culture Part 1. A study on the toxic substance in the serum of cancer patients
kn-title=骨髄組織培養法を応用せる癌毒性物質に就ての研究 第一編 癌患者血清内の毒性物質に就ての研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author studied the toxic substance in the serum of patients with malignant tumors frequently encountered in the field of internal medicine such as gastric cancer as a principal one and liver cancer, cancer of large intestine, and lymphosarcoma by observing the tissue growth and neutrophil function in rabbit bone-massow tissue cnlture and also the erythrocyte series by culture in the fluid medium. The following are the results. 1. The tissue growth in the case of the bone-marrow tissue culture loaded with the serum of cancer patients is diminished as compared with that of the control. 2. The wandering velocity of pseudeoesinophils in the majority of the cases loaded with the serum of cancer patients is lower than that of the control. 3. As for the vital staining of pseudoeosinophils in the cases loaded with cancer patient's serum, the cells are stained deeper at an earlier stage as compared with the control, proving the diminution of the cell functions. 4. In the fluid medium culture loaded with cancer patient's serum erythrocytes generally tend to increase in number to a greater extent than in the control, but the Hb content on the whole is diminished. 5. From these findings it is assumed that the toxic substance contained in cancerous sera brings about the diminution of the leucocyte functions by its direction on the bone marrow while the erythrocyte series besides its direct action on the bone marrow there is other principal factor.
en-copyright=
kn-copyright=

en-aut-name=TanakaToshio
en-aut-sei=Tanaka
en-aut-mei=Toshio
kn-aut-name=田仲俊雄
kn-aut-sei=田仲
kn-aut-mei=俊雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-2
article-no=
start-page=5163
end-page=5172
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590815
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Influences of the Bacterial Infection upon the Hematopoietic Mechanism in Bone Marrow chiefly by means of Bone-Marrow Tissue Culture Part 3. Results of the Bone-Marrow Tissue Culture of Patients with Subacute Bacterial Endocarditis
kn-title=細菌感染の骨髄造血機転に及ぼす影響に関する研究―主として骨髄組織培養による― 第3編 悪急性細菌性心内膜炎患者の骨髄組織培養成績に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the bone-marrow tissue culture of the patients with subacute bacterial endocarditis the author obtained the following results: 1. In the case of cover-slip culture, the relative growth rate is normal both before and and after the treatment of patients, but the cell-density index is normal or in some cases with a decrease in bone-marrow nucleated cells it is diminished. 2. In the case of the tissue culture in a fluid medium, the increasing rate of erythrocytes, reticulocytes, and hemoglobin is markedly low before the treatment, but it recovers with treatment. 3. The wandering velocity of neutrophils in the bone marrow has been found to be normal or slightly accelerated. 4. The carbon-particle phagocytotic ability of neutrophils in the bone marrow is markedly decreased before the treatment, but it recovers to normal when the patient is treated. 5. As for the neutral-red vital staining of bone-marrow neutrophils, these cells stain quickly and deeply, indicating a decrease in the functions, but this condition is restored to normal by treatment. In the present paper the author stated that the examinations of the bone marrow by tissue culture with emphasis on the carbon-particle phagocytosis served a quite significant purpose for clinical course observations.
en-copyright=
kn-copyright=

en-aut-name=NaitoTakakazu
en-aut-sei=Naito
en-aut-mei=Takakazu
kn-aut-name=内藤孝和
kn-aut-sei=内藤
kn-aut-mei=孝和
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-1
article-no=
start-page=4849
end-page=4862
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological Studies on Human Ascites by Tissue Culture Part 3. A study on non-tumorous ascites
kn-title=体外組織培養法による人腹水の細胞学的研究 第3編 非腫瘍性疾病時腹水に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the vital observations on 19 cases of liver cirrhosis, 4 cases of tuberculous peritonitis, 3 cases of nephrosis, and two cases of cardiac insufficiency by means of tissue culture of ascites of respective group, the auther obtained the following results: 1. In the case of arcites of liver cirrhosis, the average cell count is 219/m?, and aside from phagocytes the appearance (average of 2.7%) of phagocytic sigenet-ring cells, a marked increase (average, 9.2%) of serous cells, a slight increase (average, 2.2%) of neutrophils can be recognized. The intermediatesize phagocytes have peculiar grass-like pseudopodia and serous cells have tongue-shaped pseudo-podia that are moving actively. 2. In the case of tuberculous peritonitis, the average cell count is 1, 820/mm3. There can be recognized such charachteristics as a marked increase (average, 51.7%) of small-size phagocytes, an increase (average, 39.9%) of lymphocytes, and the appearance of intermediatesize phagocytes shapedlike sea urchins as well as lymphocytes that move more actively six hours after the start of culture than at the initial stage of culture. 3. In the case of nephrosis an increase of small-size phagocytes and a slight increase of serous cells, but most of cells are degenerated, and also fibroblast-like degenrative intermediate-size phagocytes can be observed. 4. In the case of cardiac insufficiency, a slight increase of neutrophils can be observed, but the most outstanding characteristic in this case is an early degeneration of all cells. 5. As can be seen from the above, in the vital observations each kind of non-tumorous ascites has its own cytological charateristic traits and by these traits it is definitely possible to distinguish tumorous ascites from non-tumorous ones. Therefore, the clinical application of such vital observation offers a great promise in the differential diagnosis of ascites.
en-copyright=
kn-copyright=

en-aut-name=Iri?Etsuro
en-aut-sei=Iri?
en-aut-mei=Etsuro
kn-aut-name=入江悦郎
kn-aut-sei=入江
kn-aut-mei=悦郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-1
article-no=
start-page=4839
end-page=4847
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological Studies on Human Ascites by Tissue Culture Part 2. A study on tumor ascites
kn-title=体外組織培養法による人腹水の細胞学的研究 第2編 腫瘍性腹水に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the vital observations of ascites cells by means of tissue culture conducted on 30 cases of tumor ascites, the author obtained the following results: 1. The average cell count is 642.5/m?. 2. The characteristics of the cell compestition are the appearance of tumor cells and tumorous signet-ring cells, a slight increase in the number of serous cells, a moderate increase of neutrophils, and the appearance of phagocytic signet-ring cells. 3. The detection of tumor cells and tumorous signet-ring cells is considered as the direct sign and the other cytological characteristics are considered as the indirect sign. 4. When the case showing less than six per cent of tumor cells is designated as group I, that over 20 par cent as group II, and hepatic cancer ascites as group III, each group presents respectively characteristic physio-chemical traits. 5. By vital observation tumorous signet-ring cells can be clearly distinguished from phagocytic signet-ring cells. 6. Cytological characteristics of different kinds of tumor cells, namely, adenoma, simple cancer, scirrhous cancer, colloid cancer, liver cancer, and coelothelioma cells, can be grasped by vital observation. 7. It has been possible to grasp the peculiarities of the cell-composition of ascites and cytological characterisitics of component cells as indirect sign, especially the characteristics differentiating tumor ascites from the ascites of liver cirrhosis.
en-copyright=
kn-copyright=

en-aut-name=Iri?Etsuro
en-aut-sei=Iri?
en-aut-mei=Etsuro
kn-aut-name=入江悦郎
kn-aut-sei=入江
kn-aut-mei=悦郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-1
article-no=
start-page=4831
end-page=4837
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological Studies on Human Ascites by Tissue Culture Part 1. On normal human ascites
kn-title=体外組織培養法による人腹水の細胞学的研究 第1編 人正常時腹水の細胞に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the observation on the tissue culture of ascites cells obtained from the ascites believed to be non-pathologic at the time of laparotomy of 5 patients with stomach ulcer, the author obtained the following results: 1. The average cell count is 1, 161/mm3. 2. These cells are largely composed of phagocytes, mixed with a few lymphocytes, serous cells, neutrophils, eosinophils, and fat cells. 3. Phagocytes occupy 94.4 per cent of the total, and the majority of them are the intermediate-size phagocytes (46.4%), followed by small-size and large-size phagocytes. 4. The pattern of pseudopodia of the intermediate-size phagocytes resembles quite closely to that of monocytes, but with lapse of the culture time it undergoes some changes. Judging from the pattern of pseudopodia, transformation of the cell body, and modes of flow of the cellular granules, this cell seems to be a close relative of blood monocyte. However, the translocation of the cell is not quite so distinct. 5. Serous cells are small in number, but under the normal condition they do not show pseudopodia formation.
en-copyright=
kn-copyright=

en-aut-name=Iri?Etsuro
en-aut-sei=Iri?
en-aut-mei=Etsuro
kn-aut-name=入江悦郎
kn-aut-sei=入江
kn-aut-mei=悦郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-1
article-no=
start-page=4639
end-page=4648
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental Studies on Bone-Marrow Tissue Culture Part 2. Observations on the cell proliferation, wandering velocity, carbonparticle phagocytosis, and vital staining according to the postmortem duration in human and rabbit bone-marrow tirsue culture
kn-title=骨髄組織培養に関する基礎的研究 第II篇 人，家兎死後時間別の細胞増生，遊走速度，墨粒貪喰能，生体染色の観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the observations carried on the cell proliferation (growth area, cell density) and cell functions (wandering velocity of neutrophils and pseudoeosinophils, carbon-particle phagocytosis, vital staining) in the bone marrow tissue culture of the ribs of human and femur of rabbit, both stored at 8°C and conducted at various lengths of the postmortem time, and obtained the following results. 1. With the lengthening of time after death the growth area, wandering velocity and carbon-particled phagocytosis tend to decrease, and as for the vital staining the average stainability is increased, indicating the fall in the cell functions. However, the density of cell distribution of human neutrophils in the growth zone is higher at 12 hoursf ater death than that immediately after death, but the growth index is also lower than that immediately after death. 2. As for the bone-marrow tissue culture according to the lengths of postmortem time, 120 hours after death in femur and 7 days in rabbits are the maximum limit permissible to enable the culture of the bone-marrow. 3. Both in human and rabbits the diminution in the bone marrow functions becames striking the period between 48 to 72 hours after death as the demarcation line.
en-copyright=
kn-copyright=

en-aut-name=Ma?daAkira
en-aut-sei=Ma?da
en-aut-mei=Akira
kn-aut-name=前田昭
kn-aut-sei=前田
kn-aut-mei=昭
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-1
article-no=
start-page=4629
end-page=4637
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental Studies on Bone-Marrow Tissue Culture Part 1. Observations on the cell proliferation, wandering velocity, carbon-particle phagocytosis and vital staining with respect to age
kn-title=骨髄組織培養に関する基礎的研究 第I篇 人，家兎年令別の細胞増生，遊走速度，墨粒貪喰能，生体染色の観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Following the technique of our department the author performed the bonemarrow tissue culture of ths human sternum and the rabbit femur in relation to age and observed the cell proliferation (growth area and the cell density), and the cell functions (the wandering velocity of neutrophils and psendoeosinophils, the carbon-particle phagocytosis and vital staining of the same), and obtained the following results.
1. Summarizing the cell proliferation in the bone-marrow tissue culture, in human the proliferation is high in the descending ordar of age 15, in the twenties, thirties, fifties, while in rabbits, it is high one, two and six months after birth followed by 5 days after birth; and below that is in the orber of one, two and three years old.
2. The wanderinh velocity coincides well with the rate of cell proliferation of both neutrophils and pseudoeosinophils, and in human it is great in the order of age 15, in the twenties, thirties, fifties, and seventies, while in rabbits it is greatest one and two months after birth, followed by 6 months, 5 days, one, two, and three years after birth. 3. The carboneparticle phagccytosis parallels with the cell proliferation and the wande ring velocity, and in human it decreases along with the advance in age, while in rabbits it is most active one, two and 6 months after birth, followed by 5 days, one, two and three years after birth. 4. As for the vital stainings the discoloration of stained granules is delayed in young age both in human and rabbits, and also the average stainability at the early stage of the culture shows a low value. Namely, in human at the age 15 the delay in the dircoloration is longest, and the discoloration occurs earlier along with the abvance in age as in the twenties, thirties, fifties, and seventies in ascending order. 5. From these results the functions of bone marrow in human is most active at the age of 15 years and it decreases along with the abvance in age, while in rabbits it is most active one to six months after birth, followed by 5 days after birth, and thereafter at one, two and three years the functions gradually decrcases.
en-copyright=
kn-copyright=

en-aut-name=Ma?daAkira
en-aut-sei=Ma?da
en-aut-mei=Akira
kn-aut-name=前田昭
kn-aut-sei=前田
kn-aut-mei=昭
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=7-2
article-no=
start-page=4237
end-page=4248
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590710
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Influences of Toxins of Salmonella Typhi, Salmonella Paratyphi A, B and Escherichia Coli on Bone-Marrow Tissue Culture Part 2. Influences of toxins of S. typhi, S. paratyphi A, B, and E. coli on pseudoeosinophils in the rabbit bone marrow and on neutrophils in the human bone marrow -the wandering velocity, carbon-particle phagocytosis, and vital staining-
kn-title=腸チフス，パラチフスA，B並びに大腸菌毒素の骨髄培養に及ぼす影響について 第2編 腸チフス，パラチフスA，B並びに大腸菌毒素の家兎骨髄内偽好酸球及び人骨髄内好中球に及ぼす影響について―遊走速度，墨粒貪喰能，生体染色―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By loading toxins of S. typhi, S. paratyphi A, B, and E. coli to the bone-marrow tissue culture of normal rabbits the author observed the influences of these toxins on the wandering velocity, carbonparticle phagocytosis, and vital staining by neutral red, and next by loading toxins of S. typhi and E. coli to the bone-marrow tissue culture of normal persons studied the influences on the wandering velocity of neutrophils; and still further by adding the serum of the patient with typhoid fever to the bone-marrow tissue culture of normal rabbits pursued the influences on the wandering velocity of pseudoeosinophils; and obtained the following results. 1. The inhibitory action of these toxins on the wandering and carbon-particle phagocytosis decreases in the order of that of S. typhi, S. paratyphi A and S. paratyphi B, and the toxin of E. coli has hardly any such action. In addition, each of these toxins at a certain concentration accelerates the carbon-particle phagocytosis. 2. There can be recognized no difference between the action of toxins of S. typhi and E. coli on the wandering velocity of the neutrophils in the bone marrow of normal persons and the same on the wandering velocity of the pseudoeosinophils in the bone marrow of normal rabbits. 3. The influences exerted on the vital staining of the pseudoeosinophils in the bone marrow of normal rabbits are exactly identical with those on the carbon-particle phagocytosis and the wanderirg velocity, and the intensity of inhibitory action of these toxins on the cell functions decreases in the order of S. paratyphi A, B, and E. coli. 4. From these it has been clarified that the toxin of S. typhi acts directly on the bone marrow and disturbs the cell function of neutrophils and pseudoeosinophils; and likewise the toxin of S. paratyphi A and B impairs these functions to the degree next to that of S. typhi; whereas the toxin of E. coli has hardly no such inhibitory action. 5. In the serum of the patient with typhoid fever there can be recognized a factor that inhibits the wandering velocity of pseudoeosinophils in the bone marrow of normal rabbits.
en-copyright=
kn-copyright=

en-aut-name=MotokuraKiyoshi
en-aut-sei=Motokura
en-aut-mei=Kiyoshi
kn-aut-name=本倉潔
kn-aut-sei=本倉
kn-aut-mei=潔
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4-1
article-no=
start-page=1395
end-page=1407
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Hypoplastic Anemia by means of Bone-Marrow Tissue Culture Part 1. Bone-Marrow Tissue Culture of Hypoplastic Anemia (cover-slipmethod)
kn-title=骨髄体外組織培養法による再生不良性貧血に関する研究 第1編 再生不良性貧血患者骨髄被覆培養に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With a view to elucidate the actual characteristics of the bone marrow in hypoplastic anemia the author performed a series of bone-marrow tissue culture of the sternum in the patients with this disease and obtained the following results: 1. Many fat cells were observed in the explant of the bone marrow, and a marked decrease in the tissue growth area and cell density were observed as compared with those of the normal persons. Moreover, differences can be recognized According to different types (by prof Hiraki) of this disease: namely, Type I (the type of blood cell arrest) shows the least decrease in the tissue growth area and cell density; Type V (type of pan-myelophthisis) the greatest decrease; and Type II (the type of maturation arrest), Type III (the type of regeneration disturbance), and type IV (the mixed type) the intermediary decrease. 2. Looking at the neutrophil function in the bone marrow, both their wandering velocity and carbon-particle phagocytosis are decreased, and as for the vital staining of neutrophils they show the lower function type in which cells are stained early but fade quickly, too. In this instance as in the case of tissue growth a similar tendency can be observed according to different types of this disease. By the bone-marrow tissue culture the tissue growth and cell function in the bone marrow are lowered, showing significant differences according to the types classified by prof. Hiraki. Therefore, they offer the findings that are definitely valuable for an early diagnosis of this disease as well as for judging the effect of treatment and prognosis.
en-copyright=
kn-copyright=

en-aut-name=UjiTetsuya
en-aut-sei=Uji
en-aut-mei=Tetsuya
kn-aut-name=宇治鉄也
kn-aut-sei=宇治
kn-aut-mei=鉄也
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4-1
article-no=
start-page=1379
end-page=1386
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Bone-Marrow Tissue Culture, the Simple Method of our Own Device Part 2. Vital Staining of Neutrophils in the Bone Marrow of Normal Persons and Patients with Various Blood Diseases
kn-title=簡易骨髄組織培養法に関する研究 第2編 健康人及び諸種血液疾患々者骨髄内好中球の生体染色に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the use of the simple bone-marrow tissue culture, the method devised in our laboratory, in the medium composed of serum and vitamin B(12), the author studied the neutral red vital staining of neutrophils in the bone marrow of both normal persons and patients with various blood diseases. These results were compared with those obtained by the conventional method using the medium composed of plasma mixed with heparin and chick embryo juice; and the author arrived at the following conclusions: 1. The average stain of mature neutrophils in the case of normal persons reaches the maximum of 1.12 within 4 hours after the addition of neutral red solution, fading gradually thereafter. 2. In the case of leukemia mature neutrophils in the bone marrow are stained more deeply at an early stage as compared with those of normal persons; and in the acute leukemia they fade at an early stage, and in the chronic leukemia they fade more slowly than those of acute leukemia. 3. In the case of hypoplastic anemia mature neutrophils in the bone marrow are stained deeper and earlier than those is normal persons, and also they fade more quickly. 4. In the case of agranulocytosis mature neutrophils are stained more lightly than those in normal persons. 5. In the case of essential hypochromic anemia mature neutrophils in the bone marrow are stained more deeply and earlier than those in normal persons, but fading more slowly. In the case of pernicious anemia these neutrophils are stained more deeply both before and after the treatment than those in normal persons, but as for the fading before and after treatment, the former is slightly faster than the latter. 6. These results indicate that, although this method is slightly inferior to the conventional method from the standpoint of neutral red vital staining, it is rather a superior method for clinical application because of its simplicity in manipulation the same as in the study of the carbon-particle phagocytosis.
en-copyright=
kn-copyright=

en-aut-name=OkameManabu
en-aut-sei=Okame
en-aut-mei=Manabu
kn-aut-name=大亀学
kn-aut-sei=大亀
kn-aut-mei=学
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4-1
article-no=
start-page=1369
end-page=1377
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Bone-Marrow Tissue Culture, the Simple Method of our Own Device Part 1. Carbon-Particle Phagocytosis of Neutrophils in the Bone Marrow of Normal Persons and Patients with Various Blood Diseases
kn-title=簡易骨髄組織培養法に関する研究 第1編 健康人及び諸種血液疾患々者骨髄内好中球の墨粒貪喰能に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By means of the simple bone-marrow tissue culture in the medium composed of serum and vitamin B(12), the method of our own device, the author studied the carbon-particle phagocytosis of neutrophils both in the bone marrow of normal persons and patients with various blood diseases, and compared these results with those obtained in the tissue culture using the conventional medium composed of plasma mixed with heparin and chick embryo juice. The results are as follows: 1. The average phagocytic capacity of mature neutrophils of healthy persons reaches the maximum of 1.03 after 6 hours, culture, and it slowly falls thereafter. 2. In the case of leukemia the phagocytic capacity of mature neutrophils in the bone marrow is lower than that in the case of normal persons, but when the same in acute leukemia is compared with that in chronic leukemia it is far lower in the former than in the latter. 3. The phagocytic capacity of mature neutrophils is greatly decreased in the case of hypoplastic anemia, and moderatery in agranulocytosis and Banti's disease. Moreover, in the case of Banti, s disease, when the serum of either normal persons or patients with this disease is used in the medium, the phagocytic capacity falls in either case, but the serum of normal persons tends to prove somewhat to reinstate the phagocytic power of mature neutrophils. 4. In the cases of essential hypochromic anemia and pernicious anema the phagocytic capacity of mature neutrophils decreases to an intermediate degree in both. 5. By our simple bone-marrow tissue culture method it is possible to observe the cells in bone marrow for quite long period of time. Moreover, when compared with the conventional method of the tissue culture, this is only slightly inferior, but from its very simplicity of manipulation, it is really more useful for the clinical application than the conventional method.
en-copyright=
kn-copyright=

en-aut-name=OkameManabu
en-aut-sei=Okame
en-aut-mei=Manabu
kn-aut-name=大亀学
kn-aut-sei=大亀
kn-aut-mei=学
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4-1
article-no=
start-page=1347
end-page=1367
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Vital Observations on the Movement of the Spceific Granules of Granulocytes in Bone-Marrow Tissue Culture Part 3. A Study on Neutrophils and Eosinophils in Normal Persons and Patients with Various Diseases
kn-title=骨髄体外組織培養に於ける顆粒球の固有顆粒運動の生態観察 第3編 健康人並びに各種疾病患者の好中球，好酸球に就て 附．全編の総括
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the bone-marrow tissue culture of the sternal bone marrow obtained from normal persons and patients with various diseases the author observed the granular movements of neutrophils and eosinophils with the aid of the phase-contrast microscope, and obtained the following results: 1. The granular movements of neutrophils and eosinophils in normal persons do not differ much from those in normal rabbits, but in the granules of neutrophils can be observed a movement more minute than in pseudoeosinophils, and the molecular movement can be recognized relatively frequently in the granules of eosinophils. 2. In neutrophils and eosinophils of the cases with hyperfunction of the thyroid gland, the granular movements are vigorous, and a marked acceleration in the motility of cells can be recognized. 3. In neutrophils and eosinophils of hypoplastic anemia. myxedema, cachexia from the stomach cancer, and chronic myelogenous leukemia the granular movement has been found to have slackened in all with a resultant decrease in the cell motility, and this decrease in the functions is most marked in hypoplastic anemia. 4. In the case of eosinophils in anchylostomiasis and bronchial asthma the acceleration of the cell motility can be recognized from the modes of the granular movements. Especially in the eosniphilic granules in anchylostomiasis an abnormal acceleration in the motility can be observed, showing a marked semi-radial linear forward movement of granules. 5. As has been noted in the previous report, the reverse-flow of granaules in the tail end at the early stage of the movement cycle can be seen frequently in the hyperfunction of the thyroid gland (neutrophils and eosinophils), bronchial asthma (eosinophils), and anchylostomiasis (eosinophils), in which already known that the cell motility is accelerated. In contrast to these. the reverse flow movement of granules in the tail end at the terminal stage of the cycle can be observed frequently in hypoplastic anemia and chronic myeolgenous leukemia (all neutrophils and eosinophils), apparetnly showing the decreased functions. 6. From the relationship between the appearance of the reverse-flow movement of grauules in the tail end and the cycle of the cell movement it is possible to estimate the conditions of the cell motility, and clinically ths application of this estimation will offer some criteria for the differential diagnosis and the prognosis of diseases.
en-copyright=
kn-copyright=

en-aut-name=AndoSaburo
en-aut-sei=Ando
en-aut-mei=Saburo
kn-aut-name=安東三郎
kn-aut-sei=安東
kn-aut-mei=三郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=7-8
article-no=
start-page=797
end-page=810
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=19890831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies of bronchial asthma Part 2. Studies of the changes in the peripheral airway of asthmatic patients by selective alveolo-bronchography (SAB)
kn-title=気管支喘息に関する研究 第2編 選択的肺胞気管支造影法による気管支喘息の末梢気道病変の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to investigate changes in the peripheral airway of asthmatics, selective aveolobronchography (SAB) was performed in 33 cases of asthma. SAB findings were studied in respect to the classification of asthma and also examined with results of pulmonary function test, bronchoalveolar lavage (BAL) and transbronchial lung biopsy. 1. Narrowing of the central airway was observed in almost all cases and 19 cases showed narrowing of the peripheral airways. An uneven appearance of the alveolar figure was observed in 11 cases. 2. Narrowing of the peripheral airway and an uneven appearance of the alveolar figure were often observed in non-atopic patients, in moderate or severe cases and in late-onset asthmatics. 3. The mean value of V(50)/V(25) was increased and the mean value of %V(25) was decreased in patients with narrowing of the peripheral airways and also in patients with uneven appearance of the alveolar figure. 4. An increase of neutrophils was noticed in the BAL fluid of patients with narrowing of the peripheral airways. 5. With histological study by TBLB, infiltration of eosinophils into the alveoli as well as mononuclear cells into alveoli and bronchioli was demonstrated in patients with narrowing of the peripheral airways.
en-copyright=
kn-copyright=

en-aut-name=NakamuraYukinobu
en-aut-sei=Nakamura
en-aut-mei=Yukinobu
kn-aut-name=中村之信
kn-aut-sei=中村
kn-aut-mei=之信
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=選択的肺胞気管支造影
kn-keyword=選択的肺胞気管支造影
en-keyword=気管支喘息
kn-keyword=気管支喘息
en-keyword=末梢気道病変
kn-keyword=末梢気道病変
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=7-8
article-no=
start-page=723
end-page=732
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=19890831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies of iron metabolism of the lung Part 1. Influence of inhaled iron on iron metabolism of an experimental welder's lung
kn-title=肺における鉄代謝に関する研究 第1編 実験的溶接工肺における吸入鉄の代謝について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to clarify the mechanism of elevated iron stores in welder's lungs, the cellular contents of bronchoalveolar lavage (BAL) fluid, the serum iron, total iron binding capacity, liver non-hemin iron and histological findings were examined in experiments using Wister rats exposed to inhalation of welding fumes for 4, 8,  Total cell counts, the percentage of neutrophils and iron laden alveolar macrophages in the BAL fluid increased after continuous inhalation, but they were decreased 3 months after inhalation. Serum iron, transferrin saturation, and liver non-hemin iron in rats exposed to inhalation for 8 and 12 weeks were significantly elevated (p<0.01). Iron stores of rats 3 months after inhalation were elevated more than levels 1 week after inhalation. Histological findings in the lung showed a slight thickening of alveolar wall with cellular infiltration. Iron was deposited predominantly in alveolar macrophages of the alveolar space and in connective tissue macrophages. Iron deposition was also seen in the red pulp of the spleen after inhalation. These data suggest that iron was highly stored in the whole body of patients with welder's lung, and that alveolar macrophages play an important role in the iron metabolism of the lung.
en-copyright=
kn-copyright=

en-aut-name=FujiwaraKanta
en-aut-sei=Fujiwara
en-aut-mei=Kanta
kn-aut-name=藤原寛太
kn-aut-sei=藤原
kn-aut-mei=寛太
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=溶接フューム
kn-keyword=溶接フューム
en-keyword=気管支肺胞洗浄
kn-keyword=気管支肺胞洗浄
en-keyword=溶接工肺
kn-keyword=溶接工肺
en-keyword=鉄過剰症
kn-keyword=鉄過剰症
en-keyword=ラット
kn-keyword=ラット
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=11-12
article-no=
start-page=1323
end-page=1332
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1990
dt-pub=199012
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the treatment for intractable asthma Part 2. Evaluation of the pharmacological action of various Kampo Medicines on lymphocyte and neutrophil functions in intractable asthmatics
kn-title=難治性喘息の治療に関する研究 第2編 重症難治性喘息におけるリンパ球および好中球に及ぼす各種漢方薬の薬理作用に関する検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The pharmacological action of Sho-saiko-to and Hange-koboku-to, components of Saiboku-to, as well as that of Sho-seiryu-to were compared with that of Saiboku-to and examined in intractable asthmatics with respect to both lymphocyte functions, including interleukin 2 (IL-2) production and IL-2 receptor expression in peripheral blood lymphocytes stimulated by Candida, and on neutrophil functions, including leukotriene C(4) (LTC(4)) and superoxide (O(2)(-)) production by peripheral blood neutrophils. The results revealed, first, that, Sho-saiko-to, Hange-koboku-to, and Sho-seiryu-to did not have significant suppressive effects on IL-2 production and IL-2 receptor expression by Candia, though Saiboku-to caused significant suppression of these same parameters. Second, after 2 or 3 months oral administration to intractable asthmatics Saiboku-to (TJ-96) suppressed LTC(4) production by peripheral blood neutrophils in response to Candida and Ca-ionophore. Third, in vitro, Saiboku-to caused dose-dependent suppression of both LTC(4) and O(2)(-) production by peripheral blood neutrophils. Sho-saiko-to and Sho-seiryu-to also had suppressive effects on both LTC(4) and O(2)(-) production, with Sho-saiko-to causing the strongest suppression among these drugs. These results indicate that Saiboku-to might be useful in the treatment of intractable asthma due to its suppressive effect on type IV allergy caused by lymphocyte activation by Candida. Moreover, its inhibitory effect of LTC(4) and O(2)(-) production by neutrophils prevents prolonged broncho-constriction and irreversible changes in small airways.
en-copyright=
kn-copyright=

en-aut-name=EdaRyosuke
en-aut-sei=Eda
en-aut-mei=Ryosuke
kn-aut-name=江田良輔
kn-aut-sei=江田
kn-aut-mei=良輔
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=難治性喘息
kn-keyword=難治性喘息
en-keyword=柴朴湯
kn-keyword=柴朴湯
en-keyword=漢方薬
kn-keyword=漢方薬
en-keyword=リンパ球
kn-keyword=リンパ球
en-keyword=好中球
kn-keyword=好中球
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=11-12
article-no=
start-page=1309
end-page=1321
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1990
dt-pub=199012
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the treatment for intractable asthma Part 1. Inhibitory effect of Saiboku-to on type IV allergy in intractable asthma
kn-title=難治性喘息の治療に関する研究 第1編 重症難治性喘息における柴朴湯 (TJ-96) の臨床劾果並びにIV型アレルギー反応に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In an effort to clarify the clinical and pharmacological effects of Saiboku-to in intractable asthmatics, the effect of a 6 month administration of Saiboku-to on the production of antigen-specific lymphokines were studied. The results revealed, first, that a significant decrease in attacks and steroid-sparing effects were obtained after 3 to 4 months administration of Saiboku-to (P<0.05). Second, peripheral blood lymphocyte blastogenesis by Candida was significantly suppressed after 3 months (P<0.05), though it was not by PHA. Moreover interleukin 2 (IL-2) production and neutrophil chemotatic activity (NCA) in peripheral blood mononuclear cells stimulated by Candida were significantly decreased after 3 months (P<0.01), but not by PHA. Third, in vitro production of both IL-2 and NCA by Candida were suppressed in a dose dependent manner and the augmented expression of IL-2 receptors by Candida was decreased by Saiboku-to. These results suggest that the action mechanism of Saiboku-to in intractable asthma seems to be the suppression of type IV allergy. Therefore Saiboku-to therapy might be a useful treatment for intractable asthma due to its relatively less serious side effects as compared with corticosteroids.
en-copyright=
kn-copyright=

en-aut-name=EdaRyosuke
en-aut-sei=Eda
en-aut-mei=Ryosuke
kn-aut-name=江田良輔
kn-aut-sei=江田
kn-aut-mei=良輔
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=難治性喘息
kn-keyword=難治性喘息
en-keyword=柴朴湯
kn-keyword=柴朴湯
en-keyword=カンジダ抗原
kn-keyword=カンジダ抗原
en-keyword=IV型アレルギー反応
kn-keyword=IV型アレルギー反応
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=9-10
article-no=
start-page=1197
end-page=1205
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1990
dt-pub=199010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on intractable factors in bronchial asthma Part 2. Effect of re-inhalation of antigen in the late asthmatic response in an animal model
kn-title=気管支喘息の重症難治化要因に関する研究 第2編 遅発型気道反応動物モデルにおける抗原再吸入の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In investigating the etiological mechanisms of intractable asthma, ascaris suum was used as a sensitizing antigen in experimental animal models of re-inhalation in the late asthmatic response (LAR). Our results suggest that marked expiratory prolongation was caused by re-inhalation of the antigen in LAR. Moreover, the number of neutrophils and eosinophils increased in BALF after re-inhalation of the antigen more in LAR than in IAR (P<0.01). Finally the level of LTC4 and LTB4 increased in the venous blood and BABF after reinhalation during the LAR. The data suggests that cellular reactions among neutrophils, eosinophils, and other cells migrating into the airway lumen are seen in LAR, and moreover, LTC4 and LTB4 may be important chemical mediators in prolonged asthmatic attacks.
en-copyright=
kn-copyright=

en-aut-name=OkiKazuhiko
en-aut-sei=Oki
en-aut-mei=Kazuhiko
kn-aut-name=沖和彦
kn-aut-sei=沖
kn-aut-mei=和彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=Actively sensitized model
kn-keyword=Actively sensitized model
en-keyword=Late asthmatic response
kn-keyword=Late asthmatic response
en-keyword=Re-inhalation
kn-keyword=Re-inhalation
en-keyword=BAL-cell
kn-keyword=BAL-cell
en-keyword=Leukotrienes
kn-keyword=Leukotrienes
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=9-10
article-no=
start-page=1187
end-page=1196
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1990
dt-pub=199010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on intractable factors in bronchial asthma Part 1. Mechanisms of the late asthmatic response in an animal model
kn-title=気管支喘息の重症難治化要因に関する研究 第1編 喘息動物モデルによる遅発型気道反応の機序に関する検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to investigate in detail the mechanism of late asthmatic responses (LAR) which seem to be involved in the development of intractable asthma, we prepared an experimental model of bronchial asthma using guinea pigs and examined neutrophil, eosinophil, and leukotriene (LTs) levels in venous blood and bronchoalveolar lavage fluid (BALF). Our results suggest that leukocytosis, and especially neutrophilia and eosinophilia is found in venous blood more with LAR than in IAR and non-attack states in control animals (P<0.01). Moreover, increased neutrophils were found in BALF in LAR compared with IAR and controls (P<0.01) and marked eosinophil infiltration was observed in the peribronchial tissue in LAR compared with IAR and control (P<0.01). Finally LTC4 levels were high in the venous blood and BALF in IAR, and LTB4 levels were also high in BALF in LAR. The data suggests that the accumulation of neutrtophils and eosinophils in peribronchial areas and release of leukotrienes in BALF play important roles in the etiology of LAR.
en-copyright=
kn-copyright=

en-aut-name=OkiKazuhiko
en-aut-sei=Oki
en-aut-mei=Kazuhiko
kn-aut-name=沖和彦
kn-aut-sei=沖
kn-aut-mei=和彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=Actively sensitized model
kn-keyword=Actively sensitized model
en-keyword=Guinea pig
kn-keyword=Guinea pig
en-keyword=Late asthmatic response
kn-keyword=Late asthmatic response
en-keyword=BAL-cell
kn-keyword=BAL-cell
en-keyword=Leukotrienes
kn-keyword=Leukotrienes
END

start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=
article-no=
start-page=1
end-page=13
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1962
dt-pub=19620425
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Radioactive Hot Spring Baths on Leukocyte Functions (Wandering Velocity and Carbon-Particle Phagocytic Ability)
kn-title=白血球機能（遊走速度並に墨粒貪喰能）より見た温泉浴の作用について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author studied the influence of a series of radioactive hot-spring baths, lasting 20 or 30 days, upon leukocyte functions in healthy male rabbits and in patients with rhenmatoid arthritis and other diseases. The radioactive hot springs used were "Hisui-no-Yu" and "Kenkyusho-sen" (the laboratory spring), both in Misasa Spa, the chemical compositions of which are deseribed in Table 1. The following results were obtained: 1) Wandering velocity of pseudo-neutrophilic leukocytes: When a series of plain water baths (41℃., for 5 minutes daily), were administered, velocity increased after the first bathing but later remained fairly constant, compared with initial levels, except for a temporary fall on the 9th day (Fig. 3). During the administration of a series of the weakly radioactive "Kenkyushosen" baths (43℃., for 5 minutes daily), it showed a slight increase during the first week, following a temporary fall after the first bathing, and a decrease during the third week, but it tended to
return to the initial level by the forth week (Fig. 1). In the course of a series of the radioactive ?Hisui-no-Yu" baths (40℃., for 10 minutes daily), it increased during the first week and tended to decrease during the second week, but returned to the initial level by the third week (Fig. 2). 2) Phagocytic ability for carbon-particles of pseudo-neutrophic leukocytes in rabbits: When plain water baths were given, phagocytic ability showed a rise on the third
day, after which it declined to the initial level and remained fairly constant to the end of the observation period (Fig. 6.). On the other hand, the phagocytic ability of the leukocytes in rabbits of the groups receiving radioactive hot-spring baths increased more and more markedly as serial bathing was continued, although it had slightly decreased immediately after the first bathing. The rise of phagocytic ability in the group bathed in "Kenkyusho-sen" was especially marked on the third and ninth days of serial bathing, and also on the tenth day after serial bathing had been discontinued (Fig. 4). This tendency was also observed in the group bathed in "Hisui-no-Yu", whose phagocytic ability was noticeable especially on the third and 14th days. The degree of the rise in phagocytic ability was demonstrably high in this group than in the former (Fig. 5). Acceleration of the phagocytic function of leukocytes in rabbits was observed up to the 40th day after the series of baths in the radioactive hot spring had been concluded. 3) From these findings. it is obvious that leukocyte function is increased by repeated bathing, but it should be kept in mind that a so-called dissociation phenomenon is present between the wandering velocity and the carbon-phagocytic ability of leukocytes in the groups bathed in hot springs: namely, wandering velocity showed a tendency to decline during the third week, while carbon particle phagocytic ability showed a marked increase during the same week. 4) In order to explore the effects of a series of radioactive hot-spring baths on leukocyte functions in patients with rheumatoid arthritis or other similar diseases (for example, back pain, fibrositis or neuritis), the author examined the wandering velocity and the phagocytic ability of neutrophils, and obtained the following results: The wandering velocity of neutrophils in patients with rheumatoid arthritis or similar diseases generally showed a tendency to increase during the adminlstration of a series of the radioactive "Kenkyusho-sen" baths at a temperature of 42〜43℃. (Fig. 7). The carbon-particle phagocytic ability of neutrophils gradually increase from about the seventh day onward and reached a maximum during the second week, in patients with back pain, fibrositis and neuritis (Fig. 8a). In patients with rheumatoid arthritis, however, it declined temporarily on the fifth to seventh days in 3 out of 5 patients but thereafter increased gradually, reaching its maximum on the 20th day (Fig. 8b). Thus, the leukocyte functions in patients with rheumatoid arthritis are accelerated by serial bathing, but the response in some patients may be a decline of carbon-particle phagocytic ability during the first week. This is probably due to the stress bathing imposes on adrenocortical functions. It is believed, on the basis of the facts described above, that follow-up examinations of leucocyte functions can afford a better understanding of the effects of radioactive hot springs on the defence mechanisms operative in living bodies.
en-copyright=
kn-copyright=

en-aut-name=InoueMasakatsu
en-aut-sei=Inoue
en-aut-mei=Masakatsu
kn-aut-name=井上正勝
kn-aut-sei=井上
kn-aut-mei=正勝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所内科
END

start-ver=1.4
cd-journal=joma
no-vol=122
cd-vols=
no-issue=2
article-no=
start-page=101
end-page=106
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Intravenous administration of nicorandil immediately before percutaneous coronary intervention can prevent slow coronary flow phenomenon
kn-title=経皮的冠動脈形成術直前のニコランジル経静脈的投与による冠動脈slow flowの抑制効果
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=KawaiYusuke
en-aut-sei=Kawai
en-aut-mei=Yusuke
kn-aut-name=河合勇介
kn-aut-sei=河合
kn-aut-mei=勇介
aut-affil-num=1
ORCID=

en-aut-name=HisamatsuKenichi
en-aut-sei=Hisamatsu
en-aut-mei=Kenichi
kn-aut-name=久松研一
kn-aut-sei=久松
kn-aut-mei=研一
aut-affil-num=2
ORCID=

en-aut-name=MatsubaraHiromi
en-aut-sei=Matsubara
en-aut-mei=Hiromi
kn-aut-name=松原広己
kn-aut-sei=松原
kn-aut-mei=広己
aut-affil-num=3
ORCID=

en-aut-name=FujimotoYoshihisa
en-aut-sei=Fujimoto
en-aut-mei=Yoshihisa
kn-aut-name=藤本良久
kn-aut-sei=藤本
kn-aut-mei=良久
aut-affil-num=4
ORCID=

en-aut-name=MiyajiKatsumasa
en-aut-sei=Miyaji
en-aut-mei=Katsumasa
kn-aut-name=宮地克維
kn-aut-sei=宮地
kn-aut-mei=克維
aut-affil-num=5
ORCID=

en-aut-name=MunemasaMitsuru
en-aut-sei=Munemasa
en-aut-mei=Mitsuru
kn-aut-name=宗政充
kn-aut-sei=宗政
kn-aut-mei=充
aut-affil-num=6
ORCID=

en-aut-name=KusanoKengo F
en-aut-sei=Kusano
en-aut-mei=Kengo F
kn-aut-name=草野研吾
kn-aut-sei=草野
kn-aut-mei=研吾
aut-affil-num=7
ORCID=

en-aut-name=OheTohru
en-aut-sei=Ohe
en-aut-mei=Tohru
kn-aut-name=大江透
kn-aut-sei=大江
kn-aut-mei=透
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=国立病院機構岡山医療センター　循環器科

affil-num=2
en-affil=
kn-affil=国立病院機構岡山医療センター　循環器科

affil-num=3
en-affil=
kn-affil=国立病院機構岡山医療センター　循環器科

affil-num=4
en-affil=
kn-affil=国立病院機構岡山医療センター　循環器科

affil-num=5
en-affil=
kn-affil=国立病院機構岡山医療センター　循環器科

affil-num=6
en-affil=
kn-affil=国立病院機構岡山医療センター　循環器科

affil-num=7
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　循環器内科学

affil-num=8
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　循環器内科学
en-keyword=冠動脈slow flow現象
kn-keyword=冠動脈slow flow現象
en-keyword=ニコランジル経静脈的投与
kn-keyword=ニコランジル経静脈的投与
en-keyword=急性冠症候群
kn-keyword=急性冠症候群
END

start-ver=1.4
cd-journal=joma
no-vol=43
cd-vols=
no-issue=2
article-no=
start-page=125
end-page=131
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20050201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Neutrophil and lymphocyte responses to oral Streptococcus in Adamantiades-Behcet's disease
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Immune reactions against microorganisms play an important pathogenic role in Adamantiades-Beh?et’s disease (ABD). We had previously obtained Streptococcus sanguinis (strain BD113-20) isolated from the oral cavity of patients with ABD. To investigate the pathogenesis of this isolate, we examined neutrophil 5 reactions and level of cytokine production by lymphocytes after stimulation with the strain. The reactions
of neutrophils were examined by chemiluminescence assay using whole blood. The
amounts of interferon gamma (IFN-g) and interleukin (IL)-4, IL-8, IL-10, and IL-12
produced by peripheral blood mononuclear cells (PBMCs) were measured by ELISA. 10 Strain BD113-20 activated neutrophils from patients with ABD and healthy volunteers, and, in addition it increased IFN-g production by lymphocytes. Lymphocyte from the patients with ABD showed a dominant T helper 1 (Th-1) immune response. Results indicated that both bacterial stimulation and host hypersensitivity might be involved in the symptoms and pathogenesis of ABD.
en-copyright=
kn-copyright=

en-aut-name=KurauchiTomomi
en-aut-sei=Kurauchi
en-aut-mei=Tomomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YokotaKenji
en-aut-sei=Yokota
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FujinamiYoshihito
en-aut-sei=Fujinami
en-aut-mei=Yoshihito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=IsogaiEmiko
en-aut-sei=Isogai
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=IsogaiHiroshi
en-aut-sei=Isogai
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OhtsukiHiroshi
en-aut-sei=Ohtsuki
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OgumaKeiji
en-aut-sei=Oguma
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=2
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry

affil-num=3
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=4
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=5
en-affil=
kn-affil=Department of Preventive Dentistry, Health Sciences University of Hokkaido

affil-num=6
en-affil=
kn-affil=Division of Animal Experimentation, Sapporo Medical University

affil-num=7
en-affil=
kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry

affil-num=8
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry
en-keyword=Adamantiades-Behcet's disease
kn-keyword=Adamantiades-Behcet's disease
en-keyword=Streptococcus sanguinis
kn-keyword=Streptococcus sanguinis
en-keyword=neutrophil
kn-keyword=neutrophil
en-keyword=chemiluminescence
kn-keyword=chemiluminescence
en-keyword=IL-8
kn-keyword=IL-8
en-keyword=T helper-1
kn-keyword=T helper-1
en-keyword=IFN-gamma
kn-keyword=IFN-gamma
en-keyword=IL-12
kn-keyword=IL-12
END

start-ver=1.4
cd-journal=joma
no-vol=61
cd-vols=
no-issue=1
article-no=
start-page=17
end-page=30
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=200702
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Biochemical characterization of reactive nitrogen species by eosinophil peroxidase in tyrosine nitration
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>It is well known that eosinophils are involved in tyrosine nitration. In this study, we evaluated tyrosine
nitration by rat eosinophils isolated from peritoneal fl uid and constituent eosinophils in the
stomach. Rat peritoneal eosinophils activated with 1 &#956;M phorbol myristate acetate (PMA) and 50
&#956;M NO2
&#65437; showed immunostaining for nitrotyrosine only in smaller cells, despite the fact that eosinophils
are capable of producing superoxide (O2?&#65437;). Free tyrosine nitrating capacity after incubation
with PMA and NO2
&#65437; was 4-fold higher in eosinophils than in neutrophils. Catalase and &#65400;- and &#65402;
-tocopherol inhibited free tyrosine nitration by reactive nitrogen species from eosinophils but not
that by peroxynitrite. Superoxide dismutase augmented free tyrosine nitration by activated eosinophils
and peroxynitrite. The concentration of nitric oxide released from eosinophils was relatively
low (0.32 &#956;M/106 cells/h) and did not contribute to the formation of nitrotyrosine. On the other hand,
most constituent eosinophils constituent in the rat stomach stimulated by PMA and NO2
&#65437; showed
tyrosine nitration capacity. These results suggest that intact cells other than apoptotic-like eosinophils
eluted in the intraperitoneal cavity could not generate reactive species responsible for nitration
by a peroxidase-dependent mechanism. In contrast, normal eosinophils in the stomach were capable
of nitration, suggesting that the characteristics of eosinophils in gastric mucosa are diff erent from
those eluted in the peritoneal cavity.</p>
en-copyright=
kn-copyright=

en-aut-name=TakemotoKei
en-aut-sei=Takemoto
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OginoKeiki
en-aut-sei=Ogino
en-aut-mei=Keiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WangDa-Hong
en-aut-sei=Wang
en-aut-mei=Da-Hong
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakigawaTomoko
en-aut-sei=Takigawa
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KurosawaCarmen M.
en-aut-sei=Kurosawa
en-aut-mei=Carmen M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KambayashiYasuhiro
en-aut-sei=Kambayashi
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HibinoYuri
en-aut-sei=Hibino
en-aut-mei=Yuri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=HitomiYoshiaki
en-aut-sei=Hitomi
en-aut-mei=Yoshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=IchimuraHiroshi
en-aut-sei=Ichimura
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Kanazawa University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Kanazawa University

affil-num=7
en-affil=
kn-affil=Kanazawa University

affil-num=8
en-affil=
kn-affil=Kanazawa University

affil-num=9
en-affil=
kn-affil=Kanazawa University
en-keyword=eosinophil peroxidase
kn-keyword=eosinophil peroxidase
en-keyword=reactive nitrogen species
kn-keyword=reactive nitrogen species
en-keyword=nitrotyrosine
kn-keyword=nitrotyrosine
END

start-ver=1.4
cd-journal=joma
no-vol=61
cd-vols=
no-issue=5
article-no=
start-page=239
end-page=245
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=200710
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=STAT Proteins in Innate Immunity during Sepsis: Lessons from Gene Knockout Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The innate immune system provides immediate defense against infection and serves as the first line of host defense during infection. In innate immunity, leukocytes such as neutrophils and macrophages recognize and respond to pathogens in a non-specific manner. Therefore, the recruitment and activation of leukocytes are essential in innate immunity, and are governed by a variety of chemical mediators including cytokines. Cytokines are generally divided into 2 types, termed type-1 and type-2 cytokines. Type-1 cytokines are important in local host defense, while type-2 cytokines play a protective role when inflammatory response spreads to the body. These cytokines exert their biological functions through the janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway. STAT1/3/4/6 are transcription factors that mediate IFNgamma/IL-10/IL-12/IL-13 cytokine signaling, respectively. Evidence indicates that STAT proteins have a significant impact on innate immunity during sepsis. This review focuses on recent understandings in the regulation of innate immunity by STAT proteins during sepsis and septic shock. The suppressor of cytokine signaling (SOCS) proteins are a family of SH2 domain-containing cytoplasmic proteins that complete a negative feedback loop to attenuate signal transduction from cytokines that act through the JAK/STAT pathway. The participation of SOCS proteins in sepsis is also discussed.
en-copyright=
kn-copyright=

en-aut-name=MatsukawaAkihiro
en-aut-sei=Matsukawa
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
en-keyword=cytokines
kn-keyword=cytokines
en-keyword=innate immunity
kn-keyword=innate immunity
en-keyword=sepsis
kn-keyword=sepsis
en-keyword=SOCS
kn-keyword=SOCS
en-keyword=STAT
kn-keyword=STAT
END

start-ver=1.4
cd-journal=joma
no-vol=57
cd-vols=
no-issue=1
article-no=
start-page=13
end-page=19
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2003
dt-pub=200302
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Protective effect of leukotriene receptor antagonist montelukast on smoking-induced lung injury in Wistar rats.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Increased activation of alveolar macrophage, neutrophil and mast cell has been proven in cigarette smoking (CS)-related lung disorders (CSLD). An increased production of cysteinyl-leukotrienes (LTs), which are mediators secreted from the mentioned cells, in response to CS has been shown in humans. The protective effect of LT1 receptor-1 antagonist (LTR-1AT) on CSLD is, however, not known. In this study we aimed to determine whether there is any protective effect of a LTR-1AT, montelukast (MK), on CSLD in Wistar rats. Nine controls and twenty-three smoke-exposed rats were enrolled into this study. Controls were exposed to non-filtered air, and the smoke-exposed rats were exposed to CS for 6 h/day, 6 days/week for three weeks. The CS-exposed rats were also treated with 0.1 mg/kg/day of MK or saline. Morphometric criteria for lung injury were determined as the mean linear intercept of alveolar septa (Lm), the volume density of alveolar septa (Vvspt) and the density of the alveolar surface area per unit volume of lung parenchyma (Sva.pa). Lung mast cells (LMC), which are a major source of LTs, were also counted. Results showed that Lm of the control group was significantly lower and Vvspt, Sva.pa of the controls were significantly higher compared to those of the CS-exposed groups. Animals treated with MK had significant protection against CSLD. Lm was significantly higher and Vvspt, Sva.pa were lower in the saline group than in the MK-treated group. The number of LMC in the CS-exposed groups was also significantly higher than that in the control group. Based on these results, one can suggest that some part of the pathogenesis of CSLD may be related to an enhanced LTs synthesis and LTR-1AT. Therefore, montelukast may protect against active or passive smoking-induced lung injury and related disorders.</p>

en-copyright=
kn-copyright=

en-aut-name=YukselHasan
en-aut-sei=Yuksel
en-aut-mei=Hasan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OzbilginKemal
en-aut-sei=Ozbilgin
en-aut-mei=Kemal
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=CoskunSenol
en-aut-sei=Coskun
en-aut-mei=Senol
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TugluIbrahim
en-aut-sei=Tuglu
en-aut-mei=Ibrahim
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Celal Bayar University

affil-num=2
en-affil=
kn-affil=Celal Bayar University 

affil-num=3
en-affil=
kn-affil=Celal Bayar University 

affil-num=4
en-affil=
kn-affil=Celal Bayar University
en-keyword=cigarette smoking
kn-keyword=cigarette smoking
en-keyword=cysteiny-leukotrine
kn-keyword=cysteiny-leukotrine
en-keyword=lung
kn-keyword=lung
en-keyword=montelukast
kn-keyword=montelukast
en-keyword=rats
kn-keyword=rats
END

start-ver=1.4
cd-journal=joma
no-vol=46
cd-vols=
no-issue=5
article-no=
start-page=381
end-page=387
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=199210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Asthma classification by pathophysiology and IgE-mediated allergic reaction: new concepts for classification of asthma.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Bronchial asthma was classified by the pathophysiology and by the mechanism of onset of the disease. Forty asthmatics who had serum IgE levels lower than 200 IU/ml were evaluated by two classification methods. 1. In asthma classified by a score based on clinical findings and examinations, the characteristics of the findings and examination results were compared among three asthma types, i.e., Ia. simple broncho-constriction type, Ib. bronchoconstriction+hypersecretion type, and II. bronchiolar obstruction type. Type Ib patients, in addition to manifesting hypersecretion, had a significantly higher proportion of eosinophils in the bronchoalveolar lavage (BAL) fluid compared to other asthma types. Significantly decreased values for ventilatory parameters and an increased proportion of BAL neutrophils were found in type II compared with other asthma types. 2. In a new classification by mechanism of onset, asthma was classified into three types according to the degree of participation of IgE-mediated reactions associated with specific IgE antibodies and serum levels of total IgE: asthma induced by definite IgE-mediated reaction (atopic asthma), possible IgE-mediated reactions (asthma), and asthma induced by non-IgE-mediated reaction (asthma syndrome).</p>

en-copyright=
kn-copyright=

en-aut-name=TanizakiYoshiro
en-aut-sei=Tanizaki
en-aut-mei=Yoshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KitaniHikaru
en-aut-sei=Kitani
en-aut-mei=Hikaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OkazakiMorihiro
en-aut-sei=Okazaki
en-aut-mei=Morihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MifuneTakashi
en-aut-sei=Mifune
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MitsunobuFumihiro
en-aut-sei=Mitsunobu
en-aut-mei=Fumihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HonkeNaoko
en-aut-sei=Honke
en-aut-mei=Naoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KusauraYasuharu
en-aut-sei=Kusaura
en-aut-mei=Yasuharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KimuraIkuro
en-aut-sei=Kimura
en-aut-mei=Ikuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama University 

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University 

affil-num=8
en-affil=
kn-affil=Okayama University
en-keyword=asthma
kn-keyword=asthma
en-keyword=asthma syndrome
kn-keyword=asthma syndrome
en-keyword=classification by pathophysiology
kn-keyword=classification by pathophysiology
en-keyword=classification by mechanism of onset
kn-keyword=classification by mechanism of onset
END

start-ver=1.4
cd-journal=joma
no-vol=46
cd-vols=
no-issue=4
article-no=
start-page=295
end-page=301
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=199208
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Asthma classification by a score calculated from clinical findings and examinations in subjects sensitive to inhalant allergens.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Twenty-one patients with atopic asthma were classified into three types according to their symptoms (clinical diagnosis): Ia, simple bronchoconstriction; Ib, bronchoconstriction + hypersecretion; and II, bronchiolar obstruction, and this classification was compared with a classification made according to clinical findings and examinations (score diagnosis). Type Ib asthma was characterized by the increased incidence of eosinophils in bronchoalveolar lavage fluid (BALF), while type II was characterized by ventilatory dysfunction in small airways and the increased incidence of neutrophils in BALF. Four patients, whose expectoration was between 50 and 99ml/day, of the 12 with type Ia assessed by clinical diagnosis were evaluated as type Ib by score diagnosis. One patient with type II by clinical diagnosis was assessed as questionable type II by score diagnosis. In the other 16 patients, the clinical and score diagnoses were the same.
en-copyright=
kn-copyright=

en-aut-name=TanizakiYoshiro
en-aut-sei=Tanizaki
en-aut-mei=Yoshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KitaniHikaru
en-aut-sei=Kitani
en-aut-mei=Hikaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OkazakiMorihiro
en-aut-sei=Okazaki
en-aut-mei=Morihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MifuneTakashi
en-aut-sei=Mifune
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MitsunobuFumihiro
en-aut-sei=Mitsunobu
en-aut-mei=Fumihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanimizuMasakuni
en-aut-sei=Tanimizu
en-aut-mei=Masakuni
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HonkeNaoko
en-aut-sei=Honke
en-aut-mei=Naoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KusauraYasuharu
en-aut-sei=Kusaura
en-aut-mei=Yasuharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OchiKoji
en-aut-sei=Ochi
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HaradaHideo
en-aut-sei=Harada
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SodaRyo
en-aut-sei=Soda
en-aut-mei=Ryo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=TakahashiKiyoshi
en-aut-sei=Takahashi
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KimuraIkuro
en-aut-sei=Kimura
en-aut-mei=Ikuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama University

affil-num=10
en-affil=
kn-affil=Okayama University

affil-num=11
en-affil=
kn-affil=Okayama University

affil-num=12
en-affil=
kn-affil=Okayama University

affil-num=13
en-affil=
kn-affil=Okayama University
en-keyword=bronchial asthma
kn-keyword=bronchial asthma
en-keyword=classification
kn-keyword=classification
en-keyword=ventilatory function
kn-keyword=ventilatory function
en-keyword=cellular composition of BALF
kn-keyword=cellular composition of BALF
en-keyword=sore diagnosis
kn-keyword=sore diagnosis
END

start-ver=1.4
cd-journal=joma
no-vol=18
cd-vols=
no-issue=6
article-no=
start-page=321
end-page=332
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=196412
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A histochemical study of hydrolytic and oxidative enzymes in an eosinophilic granuloma of parotid gland region
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We experienced a case of eosinophilic granuloma in soft tissue, and demonstrated its patterns of hydrolytic and oxidative enzymes histochemically. Neutrophils were rich in acid phosphatase and glucose-6-phosphate dehydrogenase.
Eosinophils had much acid phosphatase and less other hydrolytic and oxidative enzymes. Lymphocytes showed weak reaction in all enzymes. Lymph follicles and histiocytes or fibrocytes had moderately oxidative enzymes. Small blood vessels and collagen fibers were rich in alkaline phosphatase and had a moderate amount of oxidative enzymes and acid phosphatase.</p>

en-copyright=
kn-copyright=

en-aut-name=KawashimaTakao
en-aut-sei=Kawashima
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NobutoHideo
en-aut-sei=Nobuto
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SeitoTakashi
en-aut-sei=Seito
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OgataTakuro
en-aut-sei=Ogata
en-aut-mei=Takuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=37
cd-vols=
no-issue=5
article-no=
start-page=417
end-page=421
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=198310
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Superoxide anion (O2-) production by neutrophils in refractory anemia with excess of blasts.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The O2- production by neutrophils was examined in 4 cases of refractory anemia with excess of blasts (RAEB) in order to evaluate the possible causes of enhanced susceptibility to infection and to gain some informations on the differentiation of neutrophils in this hematological disorder. In three of the four RAEB cases there was little O2- production by neutrophils, in addition to there being morphological anomalies of the neutrophils such as a Pelger-Huet-like anomaly, granular deficiency and binucleated cells. These results suggest that the impairment of O2- production by neutrophils in RAEB is one of the possible causes of susceptibility to infection and also suggest that the differentiation of neutrophils in this hematological disorder is faulty. The estimation of O2- production by neutrophils may be a useful diagnostic method for preleukemia.</p>

en-copyright=
kn-copyright=

en-aut-name=TakahashiIsao
en-aut-sei=Takahashi
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KohiFumikazu
en-aut-sei=Kohi
en-aut-mei=Fumikazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=InagakiNoritoshi
en-aut-sei=Inagaki
en-aut-mei=Noritoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OhmotoEijiro
en-aut-sei=Ohmoto
en-aut-mei=Eijiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=FukumotoMitsuhiro
en-aut-sei=Fukumoto
en-aut-mei=Mitsuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=WatanabeSeiichiro
en-aut-sei=Watanabe
en-aut-mei=Seiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakaokaKazuko
en-aut-sei=Takaoka
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KitajimaKoichi
en-aut-sei=Kitajima
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KimuraIkuro
en-aut-sei=Kimura
en-aut-mei=Ikuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=SanadaHiroshi
en-aut-sei=Sanada
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama University

affil-num=10
en-affil=
kn-affil=Okayama University
en-keyword=superoxide anion
kn-keyword=superoxide anion
en-keyword= refractory anemia with excess of blasts (RAEB)
kn-keyword= refractory anemia with excess of blasts (RAEB)
en-keyword=preleukemia
kn-keyword=preleukemia
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=1
article-no=
start-page=22
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1960
dt-pub=196004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A study on the cytomorphologic structure of blood cells by vital staining I. Normal human blood cells in the bone marrow
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Vital observation on the cellular morphology of the normal human blood cells was conducted by means of bone marrow culture successfully in conjunction with vital staining with Janus green B and neutral red. A special attention was paid for the alterations of the cellular structures in the course of the culture. The findings are summarized as follows : 1) Intracellular particles with affinity to Janus green B or neutral red were classified into minute granules, granules, vacuoles, and mitochondria.
Morphologic features of each type of the particles were studied in detail. 2) Two types of granules are present in neutrophilic and eosinophilic blood cells, whereas one type of granules is present in basophilic blood cells. Eosinophilic and basophilic granules show characteristic pole formation in them at the terminal stage of the staining. 3) The rosette formation in the mature monocyte and the aggregations of neutral red vacuoles in the mature neutrophil and the mature lymphocyte were characterized. 4) The cluster of neutral red vacuoles is characteristic of the erythroblast. 5) The mitochondria of the mature neutrophil and the mature monocyte participate in producing neutral red vacuoles.</p>

en-copyright=
kn-copyright=

en-aut-name=OtaZensuke
en-aut-sei=Ota
en-aut-mei=Zensuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=63
cd-vols=
no-issue=6
article-no=
start-page=339
end-page=348
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200912
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of Sulfur Amino Acids on Tyrosyl or Serine/Threonine Phosphorylation and Translocation of Cytosolic Compounds to Cell Membrane in Stimulus-treated Human Neutrophils
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We investigated the effects of various sulfur amino acids on the phosphorylation of proteins and the translocation of cytosolic compounds to cell membrane in stimulus-treated human neutrophils using specific monoclonal antibodies. D,L-homocysteine and D,L-homocysteine-thiolactone enhanced fMLP-induced tyrosyl phosphorylation of proteins and the translocation of p47phox, p67phox, and rac to the cell membrane in a concentration-dependent manner. L-cystathionine, NAc-L-cysteine and carboxymethylcysteine suppressed the tyrosyl phophorylation and translocation of cytosolic compounds to the cell membrane. L-cystathionine, L-cysteine and NAc-L-cysteine suppressed PMA-induced serine/threonine phosphorylation and the translocation of cytosolic compounds to the cell membrane. L-cysteine, NAc-L-cysteine and D,L-homocysteine enhanced AA-induced serine/threonine phosphorylation and the translocation of cytosolic compounds to the cell membrane, but L-cystathionine had opposite effects. These results indicated that the effects of sulfur amino acids on tyrosyl or serine/threonine phosphorylation and the translocation of p47phox, p67phox, and rac to the cell membrane in the stimulus-treated human neutrophils were in parallel with those of the stimulus-induced superoxide generation reported in previous paper. L-cysteine, D,L-homocysteine and L-cystathionine weakly inhibited lipid peroxidation, but the other sulfur amino acids tested had no effect.</p>
en-copyright=
kn-copyright=

en-aut-name=AbeHidehiro
en-aut-sei=Abe
en-aut-mei=Hidehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=LiuGang
en-aut-sei=Liu
en-aut-mei=Gang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ChiHaidong
en-aut-sei=Chi
en-aut-mei=Haidong
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HeWenfei
en-aut-sei=He
en-aut-mei=Wenfei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KitaokaNoriko
en-aut-sei=Kitaoka
en-aut-mei=Noriko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YamashitaKoichi
en-aut-sei=Yamashita
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KodamaHiroyuki
en-aut-sei=Kodama
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Anesthesiology and Critical Care Medicine, Kochi Medical School

affil-num=2
en-affil=
kn-affil=Department of Anesthesiology and Critical Care Medicine, Kochi Medical School

affil-num=3
en-affil=
kn-affil=Department of Anesthesiology and Critical Care Medicine, Kochi Medical School

affil-num=4
en-affil=
kn-affil=Department of Anesthesiology and Critical Care Medicine, Kochi Medical School

affil-num=5
en-affil=
kn-affil=Department of Anesthesiology and Critical Care Medicine, Kochi Medical School

affil-num=6
en-affil=
kn-affil=Department of Anesthesiology and Critical Care Medicine, Kochi Medical School

affil-num=7
en-affil=
kn-affil=Department of Anesthesiology and Critical Care Medicine, Kochi Medical School
en-keyword=sulfur amino acids
kn-keyword=sulfur amino acids
en-keyword=phosphorylation
kn-keyword=phosphorylation
en-keyword=superoxide
kn-keyword=superoxide
en-keyword=cytosolic compounds
kn-keyword=cytosolic compounds
en-keyword=human neutrophils
kn-keyword=human neutrophils
END

start-ver=1.4
cd-journal=joma
no-vol=63
cd-vols=
no-issue=4
article-no=
start-page=213
end-page=216
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200908
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Successful voriconazole treatment of invasive pulmonary aspergillosis in a patient with acute biphenotypic leukemia
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A 23-year old woman with acute biphenotypic leukemia (ABL) complained of chest pain with cough, high fever and hemoptysis during induction chemotherapy, although she had been treated with anti-biotics and micafungin. We made a clinical diagnosis of invasive pulmonary aspergillosis (IPA) based on a consolidation in the right upper lung field on a chest radiograph as well as a high level of serum beta-D-glucan (with no evidence of tuberculosis and candidiasis). We changed her treatment from micafungin to voriconazole. Later, we discovered an air-crescent sign by CT scan that supported the diagnosis of IPA. Following voriconazole treatment, clinical symptoms ceased and abnormal chest shadows improved gradually and concurrently with a recovery of neutrophils. IPA must be considered in immunocompromised patients with pulmonary infiltrates who do not respond to broad-spectrum antibiotics. Serological tests and CT findings can aid in early diagnosis of IPA, which, along with treatment for IPA, will improve clinical outcomes.</p>
en-copyright=
kn-copyright=

en-aut-name=KobayashiKoichiro
en-aut-sei=Kobayashi
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OgasawaraMasahiro
en-aut-sei=Ogasawara
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KiyamaYoshio
en-aut-sei=Kiyama
en-aut-mei=Yoshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MiyazonoTakayoshi
en-aut-sei=Miyazono
en-aut-mei=Takayoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KagawaKumiko
en-aut-sei=Kagawa
en-aut-mei=Kumiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ImaiKiyotoshi
en-aut-sei=Imai
en-aut-mei=Kiyotoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HiranoTeiichi
en-aut-sei=Hirano
en-aut-mei=Teiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KobayashiNaoki
en-aut-sei=Kobayashi
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TanimotoMitsune
en-aut-sei=Tanimoto
en-aut-mei=Mitsune
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KasaiMasaharu
en-aut-sei=Kasai
en-aut-mei=Masaharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=2
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital

affil-num=3
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital

affil-num=4
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital

affil-num=5
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital

affil-num=6
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital

affil-num=7
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital

affil-num=8
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital

affil-num=9
en-affil=
kn-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=10
en-affil=
kn-affil=Department of Hematology, Sapporo Hokuyu Hospital
en-keyword=invasive pulmonary aspergillosis
kn-keyword=invasive pulmonary aspergillosis
en-keyword=voriconazole
kn-keyword=voriconazole
en-keyword=acute biphenotypic leukemia
kn-keyword=acute biphenotypic leukemia
en-keyword=febrile neutropenia
kn-keyword=febrile neutropenia
en-keyword=?-D-glucan
kn-keyword=?-D-glucan
END

start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=2
article-no=
start-page=111
end-page=116
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=200204
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A study on intraalveolar exudates in acute mycoplasma pneumoniae infection.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Pathologic features of Mycoplasma pneumoniae infection (M. pneumonia) are generally non-specific, and the literature regarding the pathologic features of M. pneumonia with intraalveolar exudates is limited. Clinical and histopathological studies were performed in 3 patients with M. pneumonia which did not respond to erythromycin and minocycline, but all rapidly recovered after corticosteroid therapy. In pathologic findings, we observed intraalveolar exudates and focal organization in M. pneumonia, and its intraalveolar lesions were compared between M. pneumonia and bronchiolitis obliterans organizing pneumonia containing fibrin (BOOP). Immunohistochemical studies were performed using the streptavidin biotin peroxidase complex method with anti-alpha-smooth muscle actin antibody and anti-pancytokeratin AE1/AE3 antibody. In pathologic findings, more fibrin deposits in intaalveolar lesions were observed in M. pneumonia than in BOOP. In intaalveolar lesions of M. pneumonia, a larger amount of nuclear debris, more neutrophils, and more erythrocytes were noted. Myofibroblasts were observed in the organization of BOOP, while in the intaalveolar lesions of M. pneumonia, myofibroblasts were not observed. These results suggest that M. pneumonia with intraalveolar exudates responds well to corticosteroid and its intraalveolar lesions apparently differed from those in BOOP.</p>

en-copyright=
kn-copyright=

en-aut-name=YoshinouchiTakeo
en-aut-sei=Yoshinouchi
en-aut-mei=Takeo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OhtsukiYuji
en-aut-sei=Ohtsuki
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FujitaJiro
en-aut-sei=Fujita
en-aut-mei=Jiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SugiuraYoshiki
en-aut-sei=Sugiura
en-aut-mei=Yoshiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=BannoShogo
en-aut-sei=Banno
en-aut-mei=Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SatoShigeki
en-aut-sei=Sato
en-aut-mei=Shigeki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=UedaRyuzo
en-aut-sei=Ueda
en-aut-mei=Ryuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Nagoya City University

affil-num=2
en-affil=
kn-affil=Kochi Medical School

affil-num=3
en-affil=
kn-affil=Kagawa Medical University

affil-num=4
en-affil=
kn-affil=Nagoya City University

affil-num=5
en-affil=
kn-affil=Nagoya City University

affil-num=6
en-affil=
kn-affil=Nagoya City University

affil-num=7
en-affil=
kn-affil=Nagoya City University
en-keyword=exudate
kn-keyword=exudate
en-keyword=fibrin
kn-keyword=fibrin
en-keyword=Mycoplasma pneumonia
kn-keyword=Mycoplasma pneumonia
en-keyword=organizing pneumonia
kn-keyword=organizing pneumonia
en-keyword=steroid therapy
kn-keyword=steroid therapy
END

start-ver=1.4
cd-journal=joma
no-vol=53
cd-vols=
no-issue=3
article-no=
start-page=123
end-page=126
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1999
dt-pub=199906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Efficacy of lodoxamide eye drops on tear fluid cytology of patients with vernal conjunctivitis.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A double-masked, randomized, placebo-controlled study was conducted to evaluate the effectiveness of lodoxamide tromethamine 0.1% eyedrops in preventing inflammatory cell accumulation in the tear fluid of patients with vernal conjunctivitis. A 1-week baseline period was followed by 4 weeks of treatment with either lodoxamide tromethamine 0.1% ophthalmic solution or placebo in 30 symptomatic subjects with vernal conjunctivitis. Cytological evaluation of tear fluid was performed before and after the treatment. In the lodoxamide-treated group, but not in the placebo-treated group, the number of neutrophils (P = 0.051) and eosinophils (P = 0.020) in the tears significantly decreased at the end of 4 weeks when compared with baseline (Wilcoxon-signed rank test). It was concluded that lodoxamide treatment was significantly more effective than the placebo in terms of reducing inflammatory cells in the tear fluid in vernal conjunctivitis. This objective inhibition of inflammatory cells may be associated with clinical relief.</p>

en-copyright=
kn-copyright=

en-aut-name=OguzHalit
en-aut-sei=Oguz
en-aut-mei=Halit
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=BitirenMuharrem
en-aut-sei=Bitiren
en-aut-mei=Muharrem
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AslanOsman Sevki
en-aut-sei=Aslan
en-aut-mei=Osman Sevki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OzardaliIlyas
en-aut-sei=Ozardali
en-aut-mei=Ilyas
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Harran University

affil-num=2
en-affil=
kn-affil=Harran University

affil-num=3
en-affil=
kn-affil=Istanbul University

affil-num=4
en-affil=
kn-affil=Harran University
en-keyword=inflammatory cells
kn-keyword=inflammatory cells
en-keyword=lodoxamide
kn-keyword=lodoxamide
en-keyword=tear
kn-keyword=tear
en-keyword=vernal conjunctivitis
kn-keyword=vernal conjunctivitis
END

start-ver=1.4
cd-journal=joma
no-vol=47
cd-vols=
no-issue=5
article-no=
start-page=317
end-page=321
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1993
dt-pub=199310
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=IgE-mediated allergic reaction in drug-induced asthma.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Immunoallergological studies were carried out to clarify the differences between 24 patients with drug-induced asthma (DIA) and 240 with non-drug-induced asthma (non-DIA). The mean values of age, skin reaction to Candida albicans (C. albicans), serum IgE levels, specific IgE antibodies to house dust (HD) and C. albicans, bronchial sensitivity and leukotriene B4 (LTB4) synthesis from peripheral venous blood in patients with DIA were not significantly different from those in patients with non-DIA. In contrast, the frequency of positive skin reaction to HD and histamine release from peripheral basophils by anti-IgE were significantly lower in DIA than in non-DIA. These results agree with the reports that DIA was often observed in non-atopic asthma. But, the mean value of serum IgE was very high in DIA as well as in non-DIA. This result suggests that IgE mediated reaction in DIA is important. Furthermore, the proportion of neutrophils in bronchoalveolar lavage fluid (BALF) was significantly lower in DIA than in non-DIA. Our findings suggest that a decrease of intrapulmonary neutrophils might play an important role in the pathophysiology of DIA.</p>

en-copyright=
kn-copyright=

en-aut-name=KitaniHikaru
en-aut-sei=Kitani
en-aut-mei=Hikaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KajimotoKazuhiro
en-aut-sei=Kajimoto
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SugimotoKeisuke
en-aut-sei=Sugimoto
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MifuneTakashi
en-aut-sei=Mifune
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MitsunobuFumihiro
en-aut-sei=Mitsunobu
en-aut-mei=Fumihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YokotaSatoshi
en-aut-sei=Yokota
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HiramatsuJunichi
en-aut-sei=Hiramatsu
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KawarayaMasashi
en-aut-sei=Kawaraya
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TanizakiYoshiro
en-aut-sei=Tanizaki
en-aut-mei=Yoshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University 

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University 

affil-num=6
en-affil=
kn-affil=Okayama University 

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama  University
en-keyword=drug allergy
kn-keyword=drug allergy
en-keyword=aspirin-induced asthma. LgE-mediated reacion
kn-keyword=aspirin-induced asthma. LgE-mediated reacion
en-keyword=chemical mediator
kn-keyword=chemical mediator
en-keyword=bronchoalveolar lavage
kn-keyword=bronchoalveolar lavage
END

start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=6
article-no=
start-page=447
end-page=451
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1985
dt-pub=198512
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Monocyte chemiluminescence and macrophage precursors in the aged.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Age-related alterations in the host defense system have been vigorously investigated because of increased susceptibility to infection and neoplasms in the aged. Although monocyte-macrophages form a major part of the cellular defense against microorganisms, the majority of investigations has been limited to neutrophils and lymphocytes. The present study, designed to determine the influence of age on mononuclear phagocytes, revealed no significant decrease in the absolute number of blood monocytes, but did reveal a tendency for the chemiluminescence of blood monocytes to decrease (p less than 0.10) and a significant decrease in the numbers of macrophage precursors (p less than 0.05) in the aged (over 70 year old), in comparison with controls (under 40 years old). On the basis of these findings, functional alterations of monocyte-macrophages seem to participate in the increased susceptibility to infection in the aged.</p>

en-copyright=
kn-copyright=

en-aut-name=TakahashiIsao
en-aut-sei=Takahashi
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OhmotoEijiro
en-aut-sei=Ohmoto
en-aut-mei=Eijiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AoyakaShigeo
en-aut-sei=Aoyaka
en-aut-mei=Shigeo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakizawaMichihiro
en-aut-sei=Takizawa
en-aut-mei=Michihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OdaYasuhiro
en-aut-sei=Oda
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NonakaKenichi
en-aut-sei=Nonaka
en-aut-mei=Kenichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NakadaHiroyuki
en-aut-sei=Nakada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YorimitsuSeiichi
en-aut-sei=Yorimitsu
en-aut-mei=Seiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University 

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University 

affil-num=8
en-affil=
kn-affil=Okayama University
en-keyword=monocyte chemiluminescence
kn-keyword=monocyte chemiluminescence
en-keyword=macrophage precursor
kn-keyword=macrophage precursor
en-keyword=monocyte function in the aged
kn-keyword=monocyte function in the aged
en-keyword=susceptibility to infection in the aged
kn-keyword=susceptibility to infection in the aged
END

start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=1
article-no=
start-page=57
end-page=60
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1976
dt-pub=197602
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Methemoglobinemia induced by chlorphenamidine
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A 76-year old farmer ingested 100 g of chlorphenamidine (Galectron), a plant acaricle, for the purpose of suicide. Gastric lavage was performed and the patient survived. Methemoglobinemia was noted after emergency treatment and was still present at 20 hours after ingestion of the compound. The patient was lethargic for at least 50 hours. Moderate neutrophilic leukocytosis and kidney injury were observed.</p>

en-copyright=
kn-copyright=

en-aut-name=ArimaTerukatsu
en-aut-sei=Arima
en-aut-mei=Terukatsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MorookaHiroshi
en-aut-sei=Morooka
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanigawaTakashi
en-aut-sei=Tanigawa
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ImaiMasanobu
en-aut-sei=Imai
en-aut-mei=Masanobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TsunashimaTakehiko
en-aut-sei=Tsunashima
en-aut-mei=Takehiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KitaShouichi
en-aut-sei=Kita
en-aut-mei=Shouichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Mitoyo General Hospital

affil-num=3
en-affil=
kn-affil=Mitoyo General Hospital

affil-num=4
en-affil=
kn-affil=Mitoyo General Hospital

affil-num=5
en-affil=
kn-affil=Okayama University 

affil-num=6
en-affil=
kn-affil=Okayama University 
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=1
article-no=
start-page=42
end-page=50
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=195804
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Two cases of acute basophilic leukemia, with a special reference to basophiloblasts
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The two rare cases with a high fever and anemia as the chief complaints were confirmed to be acute basophilic leukemia on the basis of the following findings, namely,
1) Numerous basophiloblasts and immature basophilocytes were found in the peripheral blood and bone marrow picture, but there were few neutrophils. 2) By the culture of bone marrow in cover-slips the growth type to acute leukemia, these basophilocytes appearing in the growth zone
were clearly distinguishable from neutrophils, eosinophils or monocytes by the modus of their movement and cellular structure. 3) In vitro fluid medium culture revealed that blasts decreased in number along with lapse of time whereas immature and mature basophilocytes increased in inverse proportion. Having encountered these two rare cases of acute basophilic leukemia and being able to autopsy one of them, the authors report their case findings and confirm the distinction of basophiloblasts. Judging from these findings, the authors are of the opinion that some modification seems to be in the offering as regard the Naegeli's myeloblast theory.</p>

en-copyright=
kn-copyright=

en-aut-name=HirakiKiyoshi
en-aut-sei=Hiraki
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SibataTamotsu
en-aut-sei=Sibata
en-aut-mei=Tamotsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NabeshimaSaburo
en-aut-sei=Nabeshima
en-aut-mei=Saburo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=5
article-no=
start-page=551
end-page=566
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1971
dt-pub=197110
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Clinical and experimental studies on folic acid deficiency due to anticonvulsants. 2. Investigations on patients receiving anticonvulsants and experimental study on the effect of diphenylhydantoin on the absorption of folic acid in rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A high incidence of subnormal serum folic acid levels was observed in 48 patients receiving anticonvulsants (75 %). In peripheral blood, macrocytosis was detected in 46 % and an increase of hypersegmented neutrophils was also seen in 24 % of the patients. Correlation existed between these signs and low serum folate levels. The growth response of Lactobacillus casei and L. leichmannii was not suppressed by the addition of various anticonvulsants to the medium of the bioassay systems. Administration of 5 mg of folic acid for a month corrected macrocytosis and an increase of hypersegmented neutrophils significantly. Folic acid supply also decreased mean diameters of the nuclei of oral epithelial cells significantly. It is concluded that subclinical folic acid deficiency is common among the patients receiving anticonvulsants. Absorption of 3H.folic acid from the small intestine of rats was inhibited by large dose of diphenylhydantoin (20 mg) not by 5 mg. This fact suggests that in patients on diphenylhydantoin, the quantity balance of folic acid and diphenylhydantoin in the intestine regulates the absorption of folic acid.</p>

en-copyright=
kn-copyright=

en-aut-name=TaguchiHirokuni
en-aut-sei=Taguchi
en-aut-mei=Hirokuni
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=42
cd-vols=
no-issue=1
article-no=
start-page=15
end-page=19
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=198802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Superoxide anion production by neutrophils in myelodysplastic syndromes (preleukemia).
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Superoxide anion (O2-) production by neutrophils from 14 untreated patients with acute nonlymphocytic leukemia (ANLL) was significantly less than that of healthy controls (4.93 +/- 1.99 vx 6.20 +/- 1.53 nmol/min/10(6) neutrophils, p less than 0.05). In 10 patients with myelodysplastic syndrome (MDS), however, it was not significantly different from the control level although 6 of the 10 patients had low levels, when individual patients were compared with the lower limit of the control range. An inverse correlation between the O2- production of neutrophils and the percentage of leukemic cells in the marrow existed in ANLL (r = -0.55, p less than 0.01), but not in MDS. Three of 4 MDS patients who died of pneumonia prior to leukemic conversion showed a low level of O2- production. The impaired O2- production by neutrophils from some MDS patients, probably due to the faulty differentiation from leukemic clones, may be one of the causes of enhanced susceptibility to infection.</p>

en-copyright=
kn-copyright=

en-aut-name=TakahashiIsao
en-aut-sei=Takahashi
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=InagakiNoritoshi
en-aut-sei=Inagaki
en-aut-mei=Noritoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakadaHiroshi
en-aut-sei=Nakada
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OhmotoEijiro
en-aut-sei=Ohmoto
en-aut-mei=Eijiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakeuchiMakoto
en-aut-sei=Takeuchi
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OsadaKen
en-aut-sei=Osada
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MatsuzakiToshiaki
en-aut-sei=Matsuzaki
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=FukudaShunichi
en-aut-sei=Fukuda
en-aut-mei=Shunichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=UchidaKozaburo
en-aut-sei=Uchida
en-aut-mei=Kozaburo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KohiFumikazu
en-aut-sei=Kohi
en-aut-mei=Fumikazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=YorimitsuSeiichi
en-aut-sei=Yorimitsu
en-aut-mei=Seiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=KimuraIkuro
en-aut-sei=Kimura
en-aut-mei=Ikuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KitajimaKoichi
en-aut-sei=Kitajima
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=SanadaHiroshi
en-aut-sei=Sanada
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama University 

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama University

affil-num=10
en-affil=
kn-affil=Okayama University

affil-num=11
en-affil=
kn-affil=Okayama University

affil-num=12
en-affil=
kn-affil=Okayama University

affil-num=13
en-affil=
kn-affil=Okayama University

affil-num=14
en-affil=
kn-affil=Okayama University Hospital
en-keyword=superoxide anion production
kn-keyword=superoxide anion production
en-keyword=myelodysplastic syndrome
kn-keyword=myelodysplastic syndrome
en-keyword=preleukemia
kn-keyword=preleukemia
END

start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=1
article-no=
start-page=15
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1977
dt-pub=197702
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Electron microscope study on the relationship between macrophages of the alevolar space and spheroid alveolar epithelial cells on mice after injection of squid-ink (sepia-melanin) solution into the trachea
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The relationship between alveolar macrophages and spheroid alveolar epithelial cells was studied with the electron microscope after injection of squid-ink solution into the trachea of the mouse. At 20 hours after injection of squid-ink solution slight degeneration was evident in alveolar macrophages with sepia-melanin particles being phagocytized with partial digestion by lysosmes. Furthermore, hardly any changes were seen in mitochondria and inclusion bodies of the spheroid alveolar epithelial cells. In contrast, at one week after injection of squid-ink solution, almost all alveolar macrophages were degenerated with destruction of the ectoplasm in which the ingested sepia-melanin particles were digested by lysosomes into fine particles, and the mitochondria of spheroid alveolar epithelial cells were degenerated and the inclusion bodies were hardly formed. At three weeks after injection of squid-ink solution, alveolar macrophages as well as speroid alveolar epithelial cells showed almost complete recovery of functional structure. As the phagocyte in the alveolar space, neutrophile leucocytes were also observed in addition to the so-called alveolar macrophage.</p>

en-copyright=
kn-copyright=

en-aut-name=SuwaKiichi
en-aut-sei=Suwa
en-aut-mei=Kiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=51
cd-vols=
no-issue=4
article-no=
start-page=227
end-page=232
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1997
dt-pub=199708
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Establishment of a new myeloid cell line with i(17q) as the sole chromosomal anomaly from the bone marrow of a patient with myelodysplastic syndrome
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A new myeloid cell line, MTO-94, was established from the bone marrow of a patient with myelodysplastic syndrome (MDS). MTO-94 cells matured in culture medium without the addition of growth factors, and yielded neutrophils with pseudo-Pelger Hue&#776;t anomaly or hypersegmentation until 6 months. Ten months after the start of cell cultivation, MTO-94 consisted of myeloblasts. Surface phenotypes were as follows: CD7 90.3%, CD13 99.6%, CD33 75.6%, HLA-DR 96.3% and CD34 0.9%. The karyotype was 46, XY, i(17q). The proliferation of MTO-94 cells was enhanced by rhlL-3, G-CSF, rhGM-CSF and rhSCF but not by rhlL-6 and erythropoietin. MTO-94 cells with i(17q) might be useful in the study of biological aspects of not only MDS, but also hematological malignancies with i(17q) as the sole chromosomal anomaly.</p>

en-copyright=
kn-copyright=

en-aut-name=MizobuchiNoriko
en-aut-sei=Mizobuchi
en-aut-mei=Noriko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakahashiIsao
en-aut-sei=Takahashi
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HorimiTadashi
en-aut-sei=Horimi
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YamamotoMegumi
en-aut-sei=Yamamoto
en-aut-mei=Megumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HamadaKyoko
en-aut-sei=Hamada
en-aut-mei=Kyoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YorimitsuSeiichi
en-aut-sei=Yorimitsu
en-aut-mei=Seiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KubonishiIchiro
en-aut-sei=Kubonishi
en-aut-mei=Ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Kochi Municipal Central Hospital

affil-num=2
en-affil=
kn-affil=Kochi Municipal Central Hospital

affil-num=3
en-affil=
kn-affil=Kochi Municipal Central Hospital

affil-num=4
en-affil=
kn-affil=Kochi Municipal Central Hospital

affil-num=5
en-affil=
kn-affil=Kochi Municipal Central Hospital

affil-num=6
en-affil=
kn-affil=Kochi Municipal Central Hospital

affil-num=7
en-affil=
kn-affil=Kochi Medical School
en-keyword=isochromosome 17q
kn-keyword=isochromosome 17q
en-keyword=myeloid cell line
kn-keyword=myeloid cell line
en-keyword=myelodysplastic syndrome
kn-keyword=myelodysplastic syndrome
END

start-ver=1.4
cd-journal=joma
no-vol=50
cd-vols=
no-issue=1
article-no=
start-page=37
end-page=46
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=199602
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Factors affecting prognosis of idiopathic interstitial pneumonia.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>&#60;P&#62;Idiopathic interstitial pneumonia (IIP) is a progressive and often fatal pulmonary disorder, and evaluating the prognosis of patients with IIP has never been sufficient. Accordingly, factors including clinical features, laboratory data, cellular components in bronchoalveolar lavage (BAL) fluid and response to corticosteroid therapy were analyzed in 35 patients with IIP whose median age of respiratory onset was 60 years (range; 37-77 years). Nineteen patients (54.3%) were in the active stage of IIP and 16 of them were treated with corticosteroids. Significant prognostic factors were the neutrophil percentage in BAL fluid, interstitial shadows on chest radiograph, pulmonary function, blood oxygen level, grade of dyspnea, and disease activity at the initial examination. Patients in the active stage showed higher proportions of neutrophils and eosinophils in BAL fluid than those in the non-active stage. Despite corticosteroid therapy, the survival of patients in the active stage was significantly shorter than those in the non-active stage. Fifty percent of the patients treated with corticosteroids were regarded as responders at 1 month after the initiation of therapy; however, there was no significant difference between responders and non-responders in terms of survival time. In conclusion, disease activity and neutrophils in BAL fluid may be important predictors of the prognosis of IIP.&#60;/P&#62;</p>

en-copyright=
kn-copyright=

en-aut-name=MatsuoKiyoshi
en-aut-sei=Matsuo
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TadaShinya
en-aut-sei=Tada
en-aut-mei=Shinya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ShibayamaTakuo
en-aut-sei=Shibayama
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=UenoYoshiki
en-aut-sei=Ueno
en-aut-mei=Yoshiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MiyakeToshitugu
en-aut-sei=Miyake
en-aut-mei=Toshitugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakeharaHideki
en-aut-sei=Takehara
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KataokaMikio
en-aut-sei=Kataoka
en-aut-mei=Mikio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=HaradaMine
en-aut-sei=Harada
en-aut-mei=Mine
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KimuraIkuro
en-aut-sei=Kimura
en-aut-mei=Ikuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama Univeristy

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University 

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama University
en-keyword=idiopathic interstitial pneumonia (LLP)
kn-keyword=idiopathic interstitial pneumonia (LLP)
en-keyword=prognostic factor
kn-keyword=prognostic factor
en-keyword=corticosteroid therapy
kn-keyword=corticosteroid therapy
en-keyword= bronchoalveolar lavage(BAL)
kn-keyword= bronchoalveolar lavage(BAL)
en-keyword=disease activity
kn-keyword=disease activity
END

start-ver=1.4
cd-journal=joma
no-vol=50
cd-vols=
no-issue=5
article-no=
start-page=227
end-page=235
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=199610
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Primary Sclerosing Cholangitis in Japanese Patients: Association with Inflammatory Bowel Disease
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>To characterize primary sclerosing cholangitis (PSC) in Japanese patients and its association with inflammatory bowel disease (IBD), 155 reported cases of PSC, including 6 cases of our own, were reviewed. The prevalence of IBD was less in Japanese PSC patients than in Western patients (23% versus 62-100%). Japanese PSC patients with IBD were younger (mean age, 33.1 versus 51.8 years) and were more often women (51% versus 36%) than those without IBD. Seventy-four percent of PSC patients with IBD had extensive colonic lesions, and 89% of those developed IBD simultaneously, with or prior to PSC. There were 3 cases of neutrophilic cholangitis among the PSC patients with IBD but none in those without IBD. Based on these observations, we speculate that there may be subtypes of PSC which differ pathophysiologically.</p>

en-copyright=
kn-copyright=

en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MizunoMotowo
en-aut-sei=Mizuno
en-aut-mei=Motowo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamamotoKazuhide
en-aut-sei=Yamamoto
en-aut-mei=Kazuhide
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama Univeristy

affil-num=4
en-affil=
kn-affil=Okayama  University
en-keyword=primary sclerosing cholangitis
kn-keyword=primary sclerosing cholangitis
en-keyword=inflammatory bowel disease
kn-keyword=inflammatory bowel disease
END

start-ver=1.4
cd-journal=joma
no-vol=52
cd-vols=
no-issue=
article-no=
start-page=13
end-page=22
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Hot-Air Room Treatment on Peripheral Leucocytes in Guinea Pigs 2. Effect of 30 Min. Hot-Air Room Treatment for 22 Days on Leucocytes Count
kn-title=熱気浴のモルモット血液細胞に及ぼす影響について 第2報 : 連続熱気浴による影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Numerical changes of peripheral leucocytes after a hot-air room treatment was observed in guinea pigs during the 22 days' successive treatment. The results were as follows. 1. Number of various leucocytes was generally decreased after the hot-air room treatment, followed by recovery to the value before treatments during 22 days' observations. 2. Numbers of total leucocytes, lymphocytes and eosinophils, which were decreased after the treatment, gradually recovered to the value before treatments during the daily hot-air room treatment. On the other hand, the recovery of neutrophils or monocytes to the count before treatment was more rapidly. The value in numerical changes of neutrophils became smaller as the hot-air room treatments were performed for many days. 3. Numerical changes of basophils and Kurloff cells did not show any definite tendency after the hot-air room treatment. 4. Numbers of total leucocytes, neutrophils, lymphocytes and eosinophils were increased and monocyte count was slightly decreased after the 22days hot-air room treatment. No
change was observed in the counts of basophils and Kurloff cells.
en-copyright=
kn-copyright=

en-aut-name=TanizakiYoshiro
en-aut-sei=Tanizaki
en-aut-mei=Yoshiro
kn-aut-name=谷崎勝朗
kn-aut-sei=谷崎
kn-aut-mei=勝朗
aut-affil-num=1
ORCID=

en-aut-name=TanakaJuntaro
en-aut-sei=Tanaka
en-aut-mei=Juntaro
kn-aut-name=田中淳太郎
kn-aut-sei=田中
kn-aut-mei=淳太郎
aut-affil-num=2
ORCID=

en-aut-name=KomagoeHaruki
en-aut-sei=Komagoe
en-aut-mei=Haruki
kn-aut-name=駒越春樹
kn-aut-sei=駒越
kn-aut-mei=春樹
aut-affil-num=3
ORCID=

en-aut-name=OdaYasuhiro
en-aut-sei=Oda
en-aut-mei=Yasuhiro
kn-aut-name=小田康広
kn-aut-sei=小田
kn-aut-mei=康広
aut-affil-num=4
ORCID=

en-aut-name=NishimuraYoshiko
en-aut-sei=Nishimura
en-aut-mei=Yoshiko
kn-aut-name=西村佳子
kn-aut-sei=西村
kn-aut-mei=佳子
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学三朝分院内科

affil-num=2
en-affil=
kn-affil=岡山大学三朝分院内科

affil-num=3
en-affil=
kn-affil=岡山大学三朝分院内科

affil-num=4
en-affil=
kn-affil=岡山大学三朝分院内科

affil-num=5
en-affil=
kn-affil=岡山大学三朝分院内科
END

start-ver=1.4
cd-journal=joma
no-vol=52
cd-vols=
no-issue=
article-no=
start-page=1
end-page=11
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Hot-Air Room Treatment on Peripheral Leucocytes in Guinea Pigs 1. Effect of Single 30 Min. Hot-Air Room Treatment on Leucocytes Count
kn-title=熱気浴のモルモット血液細胞に及ぼす影響について 第1報 : 単回(30分間)熱気浴による影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effect of hot-air room treatment on peripheral leucocytes was examined in guinea pigs by observing the numerical changes after the treatment. The results were as follows. 1. Number of totalleucocytes was decreased immediately after hot-air room treatment with a room temperature of 43℃, humidity of 75-87% and rapidly increased from 30 to 120 min after the treatment. Numerical changes of neutrophils showed a same tendency as that of total leucocytes. 2. Lymphocyte count was not changed or
slightly decreased after the hot-air room treatment. 3. Number of basophils was decreased 30 min after the treatment and then increased, differing from that of eosinophils which showed a decreased tendency 120 min after
the treatment. 4. Numbers of monocytes and Kurloff cells were slightly increased after the treatment.
en-copyright=
kn-copyright=

en-aut-name=OdaYasuhiro
en-aut-sei=Oda
en-aut-mei=Yasuhiro
kn-aut-name=小田康広
kn-aut-sei=小田
kn-aut-mei=康広
aut-affil-num=1
ORCID=

en-aut-name=NishimuraKeiko
en-aut-sei=Nishimura
en-aut-mei=Keiko
kn-aut-name=西村佳子
kn-aut-sei=西村
kn-aut-mei=佳子
aut-affil-num=2
ORCID=

en-aut-name=KomagoeHaruki
en-aut-sei=Komagoe
en-aut-mei=Haruki
kn-aut-name=駒越春樹
kn-aut-sei=駒越
kn-aut-mei=春樹
aut-affil-num=3
ORCID=

en-aut-name=TanizakiYoshiro
en-aut-sei=Tanizaki
en-aut-mei=Yoshiro
kn-aut-name=谷崎勝朗
kn-aut-sei=谷崎
kn-aut-mei=勝朗
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学三朝分院内科

affil-num=2
en-affil=
kn-affil=岡山大学三朝分院内科

affil-num=3
en-affil=
kn-affil=岡山大学三朝分院内科

affil-num=4
en-affil=
kn-affil=岡山大学三朝分院内科
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=9
article-no=
start-page=1429
end-page=1439
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19560930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental and Clinical Studies on Medical Treatment of Moniliasis with Photosensitizing Dyes. Part. �U Biological trials on Treatment of Moniliasis with Photosensitizing dye NK 91
kn-title=感光色素によるモニリヤ症治療に関する基礎的並に臨床的研究 第2編 感光色素NK91号によるモニリヤ症治療に関する動物実験
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In vitro it was observed that NK 91 cxerted an excellent fungiostatic action against Candida albicans. So that, before clinical application, the author investigated about the fungiostatic action of NK 91 on experimental animals. The results were summarized as follows: 1) In the test of toxicity, mice which were given NK 91 subcutaneous injection over the certain quantity showed the symptoms of tachypnea and inactivity in the state of unrest and excitement and died finally. LD(50) in mice was 7.02 ± 0.38 mg/kg . 2) The hemolytic cation against the erythrocytes of rabbits occurred at the concentration of 5 γ / cc. It has been observed that the wandering velocity of neutrophils was moderate at the level of 1 γ / cc, slight accelerated at 10 γ / cc and slightly declined at 100 γ /cc. 3) The blood level of NK 91 in a non-oral administration maintained relatively high concentration in one to three hour, but declined rapidly with the lapse of time. 4) In respect of the protective test against the infection, it has been observed that the survival duration of experimental mice with Moniliasis were prolonged remarkably, compared with the control. 5) In therapeutic experiments, the author confirmed the remarkable decrease of Candida albicans in organs in the case treated with NK 91, compared with the control group in the case which was cultured from organs. Moreover, in the histologic investigations, the author observed the remarkable therapeutic effect in the treated group.
en-copyright=
kn-copyright=

en-aut-name=YamaguchiYoshio
en-aut-sei=Yamaguchi
en-aut-mei=Yoshio
kn-aut-name=山口義雄
kn-aut-sei=山口
kn-aut-mei=義雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=59
cd-vols=
no-issue=
article-no=
start-page=62
end-page=67
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=198808
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Allergic reactions for severe intractable asthma and spa therapy
kn-title=気管支喘息における重症難治化反応と温泉療法
en-subtitle=
kn-subtitle=
en-abstract=Reactions for severe intractable asthma and actions of spa therapy on the reactions were discussed. Reactions participatng in onset mechanism of bronchial asthma were divided into three major groups. The first group comprises
IgE-mast cell (eliciting phase), histamine (leukotrienes) (reactive phase; humoral phase), and lymphocytes-basophils-eosinophils (reactive phase; cellular phase). The second group comprises IgG-neutrophils, leukotrienes, and neutrophils-lymphocytes-macrophages. The third group is induced by non-IgE reactions, followed by PAF and eosinophils (with blood eosinophilia), and by leukotrienes and lymphocytes (without blood eosinophilia). If these reactions are really present and participate in onset mechanism of bronchial asthma, the first group could be observed in atopic asthma, the second one in late onset asthma, and the third one in so-called intrinsic asthma with blood eosinophilia and in late onset asthma without blood eosinophilia. Spa therapy has clearing actions of
airway and improves function of adrenal glands in severe intractable asthma.
kn-abstract=予後良好と言われる気管支喘息が，どのような反応系により重症難治化傾向を取るのか，そして温泉療法がどのように作用するのかについて，若干の検討を加えた。まず関与する反応系は，その惹起相，反応相（液性因子相，細胞性因子相）の組合せにより，代表的な3つの反応系に分類することができる。1）IgE−肥満細胞（惹起相）；ヒスタミン（ロイコトリエン）（液性因子相）；リンパ球，好塩基球，好酸球（細胞性因子相），2）IgG−好中球；ロイコトリエン；好中球，リンパ球，マクロファージ，3）非IgE系反応；PAF；好酸球，3'）非IgE系反応；ロイコトリエン；リンパ球。このような機序がもし存在するとすれば，1）の反応系の関与する喘息はアトピー型喘息，2）は中高年発症型喘息，3）は所謂内因性喘息，3'）は中高年発症型喘息として把握される可能性が強い。温泉療法は，気道清浄化作用および副腎皮質機能改善作用により，これらの反応系の発現を抑制する。
en-copyright=
kn-copyright=

en-aut-name=TanizakiYoshiro
en-aut-sei=Tanizaki
en-aut-mei=Yoshiro
kn-aut-name=谷崎勝朗
kn-aut-sei=谷崎
kn-aut-mei=勝朗
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部三朝分院内科
en-keyword=気管支喘息 (Bronchial asthma)
kn-keyword=気管支喘息 (Bronchial asthma)
en-keyword=難治性喘息 (lntractable asthma)　
kn-keyword=難治性喘息 (lntractable asthma)　
en-keyword=アレルギー反応 (Allergic reaction)
kn-keyword=アレルギー反応 (Allergic reaction)
en-keyword=温泉療法 (Spa therapy)
kn-keyword=温泉療法 (Spa therapy)
END

start-ver=1.4
cd-journal=joma
no-vol=59
cd-vols=
no-issue=
article-no=
start-page=37
end-page=41
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=198808
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Separation of paripheral leukocytes by counterflow centrifugation elutriation
kn-title=JE−6B　rotor（Beckman）による末梢血白血球分離の検討
en-subtitle=
kn-subtitle=
en-abstract=Separation of peripheral leukocytes was carried out in 5 healthy subjects by counterflow centrifugation elutriation
with JE-6B rotor. 1. Number of lymphocytes was predominant at low flow rate (4.5ml/min) of counterflow centrifugation
elutriation. 2. As flow rate was increased (9-10ml/min), the purity and number of basophils, and histamine content in the fraction were gradually increased. 3. The purity and number of neutrophils were gradually increased, and the purity was reached the peak at 13ml/min. 4. The purity and number of eosinophils were gradually increased at the late stage of the centrifugation elutriation. The results
obtained here might suggest that cell separation by counterflow centrifugation elutriation is more useful for experiments, especially allergy testing, in vitro.
kn-abstract=JE−6B　rotor（Beckman）を用いてcounterflow　centrifugation　elutriationによる末梢血白血球の分離を試み，若干の検討を加えた。1．flow　rateが低い場合はリンパ球が大部分を占めたが，flow　rateの増加とともに頻度は減少した。2．　flow　rateの増加とともに，好塩基球の頻度が増加し，9−10ml／minでは頻度のみでなく細胞数の増加も認められた。各fractionにおけるヒスタミン含有量も同様の傾向が認められた。3．好中球のpeakはflow　rate　13ml／minであり以後減少を示した。4．最終段階で好酸球が増加傾向を示しChamber内で最も高値を示した。以上の結果よりcounterflow　centrifugation　elutriationによる末梢血白血球細胞分離は，in　vitroにおける実験に有用な手段である可能性が示唆された。
en-copyright=
kn-copyright=

en-aut-name=SudoMichiyasu
en-aut-sei=Sudo
en-aut-mei=Michiyasu
kn-aut-name=周藤眞康
kn-aut-sei=周藤
kn-aut-mei=眞康
aut-affil-num=1
ORCID=

en-aut-name=MatsubaraFumie
en-aut-sei=Matsubara
en-aut-mei=Fumie
kn-aut-name=松原扶美恵
kn-aut-sei=松原
kn-aut-mei=扶美恵
aut-affil-num=2
ORCID=

en-aut-name=ArakiHiroyuki
en-aut-sei=Araki
en-aut-mei=Hiroyuki
kn-aut-name=荒木洋行
kn-aut-sei=荒木
kn-aut-mei=洋行
aut-affil-num=3
ORCID=

en-aut-name=KitaniHikaru
en-aut-sei=Kitani
en-aut-mei=Hikaru
kn-aut-name=貴谷光
kn-aut-sei=貴谷
kn-aut-mei=光
aut-affil-num=4
ORCID=

en-aut-name=TanizakiYoshiro
en-aut-sei=Tanizaki
en-aut-mei=Yoshiro
kn-aut-name=谷崎勝朗
kn-aut-sei=谷崎
kn-aut-mei=勝朗
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部三朝分院内科

affil-num=2
en-affil=
kn-affil=岡山大学医学部三朝分院内科

affil-num=3
en-affil=
kn-affil=岡山大学医学部三朝分院内科

affil-num=4
en-affil=
kn-affil=岡山大学医学部三朝分院内科

affil-num=5
en-affil=
kn-affil=岡山大学医学部三朝分院内科
en-keyword=逆流負荷遠心法 (Counterflow centrifugation elutriation)
kn-keyword=逆流負荷遠心法 (Counterflow centrifugation elutriation)
en-keyword=JE−6Bローター (JE-6B rotor)
kn-keyword=JE−6Bローター (JE-6B rotor)
en-keyword=細胞分離 (Cell separation)
kn-keyword=細胞分離 (Cell separation)
en-keyword=好塩基球 (Basophils)
kn-keyword=好塩基球 (Basophils)
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=8
article-no=
start-page=1191
end-page=1206
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19560831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Phagocytosis of Carbon Particles Investigated by Means of the Tissue Culture of Bone Marrow. Part �V. Phagocytosis of carbon particles of the neutrophilic leukocyte observed in the tissue cultures of bone marrow obtained from patients
kn-title=骨髄組織培養に於ける墨粒貪喰能の研究 第三編 病的骨髄内好中球の墨粒貪喰能に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The phagocytic behaviors of the neutrophilic leukocytes in the tissue cultures of sternal marrows of patients, especially of those suffering from blood diseases were investigated. Moreover, additional investigtion was made on the sera of these patients in regards to their influences upon the phagocytosis of pseudo-eosinophilic leukocyte in the tissue culture of rabbit bone marrow. The results are summarized as follow: 1) Such diseases with characteristic leukopenia were examined in the first place. Hypoplastic anemia caused a remarkable decrease in the phagocytic activity of neutrophilic leukocyte. The serum obtained from the patient of this disease, when added to the tissue culture of rabbit bone marrow, also reduced the phagocytic activity of pseudo-eosinophilic leukocyte. In case of Banti's Syndrome a considerable loss of the phagocytic activity of neutrophilic leukocyte resulted and no appreciable influences of the serum on that of pseudo-eosinophilic leukocyte of rabbit bone marrow were observed. Kala-azar and agranulocytosis induced a suppression of the phagocytic behavior of neutrophilic leukocyte, respectively. 2) In the second test dealing with leukemia, the neutrophilic leukocytes, both the myeloic as well as the monocytic ones, of bone marrow suffered from some decreases, a slight decrease in the chronic form of this disease and a heavy one in the acute form, in their phagocytic activities. Addition of the serum also caused a lowering in the phagocytic activity of pseudo-eosinophilic leukocyte of rabbit bone marrow. 3) In the third place several diseases accompanying no remarkable changes in leukocyte number were tested. Essential hypochromic anemia was found to suppress the phagocytic behavior of neutrophilic leukocyte of bone marrow. The serum also proved unfavorable for phagocytosis of pseudo-eosinophilic leukocyte. Lowering of phagocytic activity fo neutrophilic leukoeyte was seen also in hook-worm anemia and post-hemorrhagic anemia in a parallel manner to the conditions of anemia. In these two diseases the sera had no effects on the phagocytic behavior of the pseudo-eosinophilic leukocyte of rabbit bone marrow. Idiopathic thrombocytopenic purpura proved to have no appreciable effects on the phagocytic activity of neutrophilic
leukocyte and this was also the case with regard to the addition of the serum. 4) Other diseases than the blood diseases seemed to have little to do with the activities of phagocytic behaviors of the leukocytes.
en-copyright=
kn-copyright=

en-aut-name=SunamiHiroshi
en-aut-sei=Sunami
en-aut-mei=Hiroshi
kn-aut-name=角南宏
kn-aut-sei=角南
kn-aut-mei=宏
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=8
article-no=
start-page=1179
end-page=1189
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19560831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Phagocytosis of Carbon Particles Investigated by Means of the Tissue Culture of Bone Marrow. Part �U. Phagocytosis of various cells in the bone marrow of normal adult and of rabbit
kn-title=骨髄組織培養に於ける墨粒貪喰能の研究 第二編 健康人及び家兎骨髄内細胞の墨粒貪喰能に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The bone marrow tissues were cultured from normal adult and rabbit. The processes of phagocytosis were scrutinized in the cultured tissues of bone marrow. The results are as follow: 1) The majority of neutrophilic leukocytes such as myeloblast and promyelocyte but a few exceptions of myelocyte exhibited no phagocytosis. Active phagocytosis was displayed by the matured neutrophilic leukocytes more than by metamyelocytes. The pseudo-eosinophilic leukocyte of rabbit bone marrow was much weaker in its phagocytic activity than the neutrophilic leukocyte. 2) Usually no phagocytic behaviors were recognized in eosinophilie, basophilic-leukocytes, lymphocytes or erythroblasts. 3) Remarkable adsorption and phagocytosis of carbon particles were exhibited by throm-bocyte but the matured type of megakaryocyte had no phagocytic activity. 4) The phagocytic activity of monocyte was at first lower than that of neutrophilic leukocyte but gradually grew high until it surpassed the latter. This great phagocytic activity of monocyte was kept long until the end of observation. 5) The so-called "Makrophagen" showed the greatest phagocytic activiy of all the bone marrow cells. 6) In regard to the average degree of phagocytosis of matured neutrophilic leukocyte, different values were obtained from one growth zone to another. When the average degrees of phagocytosis in whole growth zones were examined along the radial direction from marrow fragment, they exhibited the highest value after 3 hours’ cultivation. Thereafter the phagocytic activity of neutrophilic leucocyte again fell down. Pseudo-eosinophilic leukocyte showed its highest value of the average degree of phagocytosis after 6 hours’ cultivation. 7) Lowerings of phagocytic activity were observed when Nitromin or P(32) were added to the medium and these lowerings of activity were nearly proportional to the concentrations of these substances. This seems to point out some part of considerable importance which underlies the functions of bone marrow.
en-copyright=
kn-copyright=

en-aut-name=SunamiHiroshi
en-aut-sei=Sunami
en-aut-mei=Hiroshi
kn-aut-name=角南宏
kn-aut-sei=角南
kn-aut-mei=宏
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=8
article-no=
start-page=1169
end-page=1178
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=19560831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Phagocytosis of Carbon Particles Investigated by Means of the Tissue Culture of Bone Marrow. Part �T. Comparative study on the methods and general considerations on phagocytosis of carbon particles
kn-title=骨髓組織培養に於ける墨粒貪喰能の研究 第一編 方法論並びに一般的観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Several methods for tissue culture of bone marrow were comparatively examined in order to study the mechanisms of phagocytosis of carbon particles in detail. From the results obtained here, a consideration was made on the mechanisms of phagocytosis as follow : 1) Culture method with coverslips hitherto described, when applied with the Indian ink mixed with the culture medium as well as with the ink added after the coagulation of plasma, proved insufficient for the observation with oil immersion system and for the quantitative investigation. 2) A modified procedure with Unno's slide-glass proved more successful in that quantitative study could easily be made and more datailed observation of phagocytosis with phase contrast microscope could be accomplished. 3) Phagocytosis of carbon micro particles from the culture medium by the neutrophilic leukocytes was observed very clearly, but in case of monocytes it is quite different in that the submicroscopic microgranules of carbon were chiefly adsorbed on the surface of their secretion granules. In addition, in the vacuoles of neutrophilic leukocytes Brownian movement of carbon particles was observed. This was not so in case of monocytes. 4) The continuous observation of the process of phagocytosis described here afforded a better understanding on the mechanism of phagocytosis which would not be obtained by the usual methods hitherto described such as supravital observation or the fragmental observations after the intravenous administration of Indian ink.
en-copyright=
kn-copyright=

en-aut-name=SunamiHiroshi
en-aut-sei=Sunami
en-aut-mei=Hiroshi
kn-aut-name=角南宏
kn-aut-sei=角南
kn-aut-mei=宏
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=5-1
article-no=
start-page=2283
end-page=2292
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590415
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinical and Experimental Studies on the Characteristics of Idiopathic Thrombocytopenic Purpura Part 3. The Influence of the Spleen in the Guiea Pig with Experimental Thrombocytompenia on Megakaryocytes
kn-title=特発性栓球減少性紫斑病の本態に関する臨床的並びに実験的研究 第3編 骨髄巨核球機能に及ぼす実験的栓球減少症海?脾の影響に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In an attempt to study the relationship between the megakaryocyte function and the spleen in experimental thrombocytopenia, the author performed the tissue culture of the bone marrow and the spleen of normal guinea pigs and also the bone-barrow tissue culture of normal guine pigs side by side along with the spleen of the guinea pigs injected with anti-platelet serum on the same slide glass, each by a simple culture method; and observed the number and functions of megakaryocytes appearing in the growth area and the wandering velocity of neutrophils. 1. In the case of the bone-marrow tissue of normal guinea pigs cultured side by side with normal spleen on the same slide, megakaryocytes appering in the growth area was somewhat less in number and their functions were also lower than in the case of the bone-marrow tissue cultuse alone. However, there was no significant difference in the wandering velocity of neutrophils. 2. When the spleen of normal guinea pigs given anti-platelet serum was cultured side by side with bone marrow of normal guinea pigs, no significant difference in number of megakaryocytes appearing could be recognized as compared with the case with the normal spleen, but the functions were markedly decreased. The wandering velocity of neutrophils in both of these cultures was Identically the same. From these findings it has been found that in the spleen of the guinea pigs injected with anti-platelet serum there exists a factor which acts directly on megakaryocytes of the normal guinea pigs as to inhibit their platelet production.
en-copyright=
kn-copyright=

en-aut-name=HondaSeiken
en-aut-sei=Honda
en-aut-mei=Seiken
kn-aut-name=本多正憲
kn-aut-sei=本多
kn-aut-mei=正憲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=5-1
article-no=
start-page=2271
end-page=2281
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590415
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinical and Experimental Studies on the Characteristics of Idiopathic Thrombocytopenic Purpura Part 2 The Influence of the Serum of Guinea Pigs with Experimental Thrombocytopenia on the Megakaryocyte Function
kn-title=特発性栓球減少性紫斑病の本態に関する臨床的並びに実験的研究 第2編 骨髄巨核球機能に及ぼす実験的栓球減少症海?血清の影響に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With a view to study the causative factor of the experimental thrombocytopenia induced in guinea pigs by injecting anti-platelet serum into guinea pigs, the author performed the bone-marrow tissue culture of normal guinea pigs in the serum of guinea pigs with this experimental thrombocytopenia by means of the simple method of bone-marrow tissue culture, and observed the number and functions of megakaryocytes as well as the relative tissue growth rate and the wandering velocity of neutrophils. The number of megakaryocytes appearing in the tissue growth area showed no significant difference from that of the bone-marrow tissue culture in the serum of normal guinea pigs, the control, but the megakaryocyte function was markedly diminished as compared with the control, especially after 24 hours' culture no such megakaryocytes as showing the separation of platelets could at all be observed and also a marked degenerative picture was observed. The relative tissue growth rate was somewhat decreased, and the wandering velocity of neutrophils showed no significant difference from that of the control. From these findings it has become clear that in the serum of guinea pigs with experimental thrombocytopenia there exists a factor acting directly on megakarxocytes as to suppress the separation of platelets.
en-copyright=
kn-copyright=

en-aut-name=HondaSeiken
en-aut-sei=Honda
en-aut-mei=Seiken
kn-aut-name=本多正憲
kn-aut-sei=本多
kn-aut-mei=正憲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=5-1
article-no=
start-page=2261
end-page=2270
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590415
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinical and Experimental Studies on the Characteristics of Idiopathic Thrombocytopenic Purpura Part 1. The Influence of the Serum of the Patient with Idiopathic Thrombocytopenic Purpura on the Megakaryocyte Function of Normal Persons
kn-title=特発性栓球減少性紫斑病の本態に関する臨床的並びに実験的研究 第1編 特発性栓球減少性紫斑病患者血清の健康人骨髄巨核球機能に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to pursue the platelet reducing factor in the serum of idiopathic thrombocytopenic purpura, the author performed the bone-marrow tissue culture of the normal persons and the patients with this disease by the simple method of bone-marrow tissue culture; and also observed number of megakaryocytes, their functions (motility and separation of platelets) in the tissue growth area, relative tissue growthrate, and the wandering velocity of neutrophils by means of the bone-marrow tissue culture of normal persons in the serum of patient with this disease. 1. In the bone-marrow tissue culture of this disease number of megakaryocytes appearing in the tissue growth area was normal in one case, and greater than the normal in two cases; but the megakaryocyte funcion was on the whole lowered. The relative growth rate and the wandering velocity of neutrophils showed no significant differences from those of the control. 2. In the case of the bone-marrow tissue culture of normal persons in the serum of this disease there was no significant difference in the number of megakryocytes appearing in the growth area from that of the control, but the megakaryocyte function was markedly diminished and also a marked degeneration was recognized. However, the relative growth rate and the wandering velocity of neutrophils showed no significant differences from those of the control. From these findings it is clear that there exists some factor in the serum of the patient with thrombocytopenic purpura, which acts directly on megakarycoytes so as to inhibit the thrombocyte production.
en-copyright=
kn-copyright=

en-aut-name=HondaSeiken
en-aut-sei=Honda
en-aut-mei=Seiken
kn-aut-name=本多正憲
kn-aut-sei=本多
kn-aut-mei=正憲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=12
article-no=
start-page=4659
end-page=4667
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=1958
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Fixation of the Dyes used in Supravital Staining of Cells Part 4. Observations on the Various Blood Cells Supravitally Stained and Fixed by Potassium Mercuric Iodide
kn-title=超生体染色色素の固定に関する研究 第4編 各種血液細胞の観察所見
en-subtitle=
kn-subtitle=
en-abstract=By the methods reported in the previous papers the anther observed the supravitally stained granules on the nucleus stained with hematoxylin. As the results it has been revealed that in lymphocytes, monocytes, myelocytic cells and Yoshida sarcoma cells, Janus green B stains mitochondria selectively but not the other components. The irregular forms of mitochondria stained with Janus green B can be recongnized frequently. This seems to be the result of the structural demolition caused by the staining itself. But in some cells the number of mitochondria which are stained by this dye, is rather small compared with those found under the phase-contrast or the electron-microscopes. Then it is assumed that Janus green B stains mitochondria selectively but not all of them. In general neutral red seems not to stain Golgi apparatus selectively, and neutral red granules are found to be irregular, both in their distribution in cytoplasma and in their size, all the granules being almost round in shape. But in small cells like monocytes, sometimes in lymphocytes the granules tend to gather in the area of Golgi zone. This thendency is recongnized especially markedly in the cells from the lower animals In the rosette formation of the neutral red Yoshida sarcoma cells have almost the same picture as that of monocytes but their mitochondria stainable with Janus green B are larger in number compared with those of monocytes. Brilliant crecyl blue and Nile blue seem also to stain mitochondria, but some other components like endoplasmic reticulum, neutrophilic granules and others in leucocytes, which can be stained by these dyes as the stained granules are very large in number and show marked variety in their sizes.
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=MakiTeruo
en-aut-sei=Maki
en-aut-mei=Teruo
kn-aut-name=真木照雄
kn-aut-sei=真木
kn-aut-mei=照雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=11
article-no=
start-page=4165
end-page=4179
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=1958
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological Studies on the Fluid obtained by Puncture by Smear-Stain Method and Peroxidase Reaction Part 3. Cytological Studies on the Ascites and Thoracic Fluid in Various Diseases
kn-title=塗抹染色及び過酸化酵素反応による穿液刺の細胞学的研究 第3編 各種疾患々者腹水及び胸水の細胞学的研究
en-subtitle=
kn-subtitle=
en-abstract=As the results of cytological investigations cf ascites and thoracic fluid in various diseases, the author recognized the cellular elements and the staining findings characteristic to each disease; and in the application of these for the diagnosis of various diseases the author obtained the following findings that may be used as the criteria for the differential diagnosis of these diseases: 1. In the case of liver cirrhosis, serosal cells, lymphocytes and small phagocytes increase in number, and neutrophils also incrsase slightly; and of them serosal cells increase in all cases. The cell body of phagocyte, having many vacuoles and marked meshwork, in general is easily destroyed. Moreover, one half of the cases are consisted of signet-ring cells derived from phagocytes, and in some instances mitotic picture can be observed. 2. In the case of nephrosis, lymphocytes and small phagocytes increase; and in general small cells occupy the major portion of the cells. As for phagocytes, the degree of degeneration in both the nucleus and cell body is high, and hence the cell is easily destructible 3. In tuberculous disease lymphocytes and small phagocytes increase markedly, and these small cells occupy the major portion. The break-down of phagocytes is rare, and in rare instances signet-ring cells derived from phagocytes and mitotic picture can be observed. 4. In the case of heart disease lymphocytes and small phagocytes are numerous. 5. The characteristics of tumorous ascites and thoracic fluid are the appearance of tumor cells, but even in the absence of tumor cells still there exists a peculiar characteristic of its own in other kinds of cells. Namely, an increase in serosal cells, the appearance of abnormal serosal cells (many nuclei, giant nucleoli, vacuole formation, deep stain of the cell body, and indistinctness of nuclear boundary), an increase of neutrophils in some cases, and appearance of signet-ring cells, and mitotic picture. These are thought to be indirect signs of tumorous ascites and thoracic fluid, and in later examinations tumor cells have been detected in the majority of them.
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=FukudaGenjiro
en-aut-sei=Fukuda
en-aut-mei=Genjiro
kn-aut-name=福田源次郎
kn-aut-sei=福田
kn-aut-mei=源次郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=11
article-no=
start-page=4157
end-page=4163
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological Studies on the Fluid obtained by Puncture by Smear-Stain Method and Peroxidase Reaction Part 2. Essential Characteristics of Phagocytes in the Abdominal Cavity
kn-title=塗抹染色及び過酸化酵素反応による穿刺液の細胞学的研究 第2編 腹腔内食細胞の本態
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By observing monocytes and subcutaneous histiocytes in mice and rabbits, and by investigating still further phagocytes, the author discussed the similarity and dissimilarity of these cells; and also made a detailed study on essential charcteristics of phagocytes newly born in the ascites at the time of stimulation by checking periodical changes of smeared-stained specimen findings and cell counts and the peroxidase reaction; and obtained the following results: 1. Phagocytes, possessing as they do characteristics of both histiocytes and monocytes, can not conclusively be said to resemble one of the two as they themselves are. Morphological changes ocurring in phagocytes due to particular environments of ascites are responsible for this phenomenon. 2. Under a stimulation the cell count increases in ascites, and of them granulocytes and small phagocytes (newly born phagocytes) occupy a greater part, and also number of lymphocytes increases to a certain degree. 3. From findings on nuclear membrane, nuclear meshwork, chromatin, nuclear lobes, and vacuoles of the cell, new-born phagocytes present findings similar to those of immature monocytes and along with the lapse of time they present findings of mature monocytes by developing azure granules and pigmentation close to nuclear indentation; and still further vacuoles in the cell increase, and nuclear meshwork becoms irregular and the cell body grows cloudy, presenting findings similar to histiocytes, and then degenerate. Namely, phagocytes essentially possess the characteristic findings of monocytes. 4. On comparing the peroxidase reaction of phagocytes under the stimulation between that of mouse and rabbit, because the mouse with a low phagocytic power shows a higher positive percentage of the peroxidase reaction than in rabbit, peroxidase reaction granules of phagocytes are not all due to phagocytosis of neutrophils, and it has been reconfirmed the conclusion of Part 1 that in the case of mouse the peroxidase reaction is positive and in the case of rabbit negative. Although the positive rate of this reaction decreases as the cells degenerate and become aged, even then a higher positive rate is maintained in mouse than in rabbit. Therefore, this cell can be said to be positive cell. 5. From these results, it is concluded that phagocytes in the serous cavity are cells closely related to monocytes.
en-copyright=
kn-copyright=

en-aut-name=FukudaGenjiro
en-aut-sei=Fukuda
en-aut-mei=Genjiro
kn-aut-name=福田源次郎
kn-aut-sei=福田
kn-aut-mei=源次郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=11
article-no=
start-page=4141
end-page=4148
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Benzene Poisoning Part 4 Hygienic Investigation on Lacquer-Spray Workers
kn-title=Benzene中毒に関する研究 第�W編 ラッカー吹付塗装工の実態調査成績
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In May, 1956, several analyses of the atmospheric benzene concentration in spray-painting factories of motor-cars and general examinations were made on 16 workers exposed to thinner (benzene) during their working hours, and the following results were obtained. 1. The atmospheric benzene concentration in each plant was 15-96 p.p.m. at the breathing level of the laborers working at about 1 meter away from the sprayer and the concentration was about 67 p.p.m. on the average. 2. Blood examinations: An increase in the hemoglobin content was not observable, but a decrease in the red cell count was seen with a tendency towards an increase in the average diameter of the cell, and red cells indicated a picture of macrocytic-hyperchromatic anemia. A decrease in the leucocyte count was obvious, especially, neutrophil leucocytes showed the decrease in both relative percentage and absolute counts and also the leftward nuclear shift was recognized. 3. Some workers complained of fatigue, loss of bodyweight, dizziness and sleeplessness as the subjective symptoms. 4. Ethereal sulfate ratio and conjugated phenol ratio in the urine exhibited a considerable increase both before and after the work as compared with those of the control.
en-copyright=
kn-copyright=

en-aut-name=SugaharaKiyoshi
en-aut-sei=Sugahara
en-aut-mei=Kiyoshi
kn-aut-name=菅原澄
kn-aut-sei=菅原
kn-aut-mei=澄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=3-2
article-no=
start-page=1545
end-page=1562
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590315
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental and Clinical Studies on Peripheral-Leucocyte Culture Part 3. The Peripheral-Leucocyte Culture of Patients with Various Blood Diseases
kn-title=末稍血白血球培養に関する基礎的並に臨床的研究 第3編 血液疾患々者末梢血白血球培養に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By performing the peripheral-leucocyte culture of patients with various leukemias, leukemoid reaction, and hypoplastic anemia, the author observed the tissue growth and the function of neutrophils in the culture; and obtained the following results: 1. In the case of chronic myelogenous leukemia, the tissue growth is composed of double layers of growth zones, namely, one, the inner young-cell zone with a clear-cut boundary and high cell density, and the other, the outer mature-cell zone with less cell density; but the young-cell zone gradually grows smaller with the lapse of time as these young-cells mature. The function of neutrophils is slightly inferior to that in the case of normal persons. 2. In the cases of the acute myelogenous and the lymphocytic leukemias as well as monocytic leukemia, the tissue growth area is sharply demarcated, forming no outer maturecell zone. As for the cell density, it is highest in the case of lymphocytics, and in the case of monocytic it is relatively low; and with the lapse of culture time immature blasts are seem maturing, each type of cells presenting their peculiar movement pattern and thus it is possible to distinguish different types of cells. The function of neutrophils in acute form is extremely lower than that of chronic form. 3. In the slice specimens prepared during the peripheral-leucocyte culture of leukemia many mitotic pictures were recognized. 4. In the case of leukemoid reaction the pattern of tissue growth and the function of neutrophils are identical with those in the case of normal persons, making it easy to differentiate this disease from leukemia. 5. In the case of hypoplastic anemia, the tissue growth area presents a coroma-like pattern of growth the same as in the case of normal persons, but the growth area is smaller and the cell density is less, and moreover, the function of neutrophils is markedly diminished. From these results it has been proven that clinically the peripheral-leucocyte culture is sufficiently worthy of use for the differential diagnosis of various blood diseases.
en-copyright=
kn-copyright=

en-aut-name=SogawaTamotsu
en-aut-sei=Sogawa
en-aut-mei=Tamotsu
kn-aut-name=十川保
kn-aut-sei=十川
kn-aut-mei=保
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=3-2
article-no=
start-page=1537
end-page=1543
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590315
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental and Clinical Studies on Peripheral-Leucocyte Culture Part 2. The Function of Neutrophils in the Peripheral-Leucocyte Culture of Normal Persons
kn-title=末梢血白血球培養に関する基礎的並に臨床的研究 第2編 健康人末梢血白血球培養の好中球機能に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to observe the cell function of peripheral leucocytes of normal persons by means of tissue culture, the author studied the wandering velocity, carbon-particle phagocytosis and neutral-red vital staining of cells from various angles; and arrived at the following conclusions: 1. Neutrophils begin to wander about actively already after 3 hours' culture, and after 6 hours' their behavior becomes still more vigorous, showing the maximum wandering velocity at this stage; but thereafter it gradually decreases, and after 24 hours' the wandering becomes very sluggish. 2. In pursuing the carbon-particle phagocytosis of various leucocytes, neutrophils and monocytes reveal a marked phagocytosis; especially the phagocytosis of monocytes is highest, followed by that of neutrophils; but no phagocytosis can be recognized in eosinophils, basophilocytes, and lymphocytes. 3. The average phagocytic capacity of neutrophils after 6 hours' culture reaches its maximum, but thereafter it declines very slowly. 4. As for the degree of stain to neutral-red vital staining in various leucocytes, at first a few granules in neutrophils and monocytes are stained lightly, but with the laspe of time numbers of these granules gradually increase and these stained granules. swelling up by confluence, present a peculiar arrangement in a form of rosette. In eosinophils and lymphocytes all granules are stained light red, and in basophilocytes these are stained deep red. All these granules are stained almost simultaneously, but the confluence of stained granules are not so marked. 5. The neutral-red vital staining of neutrophils is quite high already at the early stage of culture, and reaching the maximum after 3 hours' culture, thereafter it gradually begins to fade and 9 hours' after it is about the same degree of stain as that immediately after the addition of neutral red. It is almost completely faded after 24 hours' culture.
en-copyright=
kn-copyright=

en-aut-name=SogawaTamotsu
en-aut-sei=Sogawa
en-aut-mei=Tamotsu
kn-aut-name=十川保
kn-aut-sei=十川
kn-aut-mei=保
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=3-2
article-no=
start-page=1529
end-page=1535
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590315
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental and Clinical Studies on Peripheral-Leucocyte Culture Part 1. Methodology and Cell multiplication in the Peripheral-Leucocyte Culture of Normal Persons
kn-title=末梢血白血球培養に関する基礎的並に臨床的研究 第1篇 方法論並に健康人末梢血白血球培養の細胞増生に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to maintain the cell function in a good condition for the peripheral-leucocyte culture the author compared various methods of tissue culture, and then investigated the tissue growth of peripheral leucocytes of normal persons in culture. The results are as follows: 1. For the preservation of blood to be cultured the use of the container whose inner surface coated with silicon is the best way to maintain the cell function as compared with the conventional method. 2. The expansion of growth area surrounding the original peripheral leucocyte mass in normal persons ceases after 6 hours' culture, and with the lapse of time the central zone of the growth area becomes less dense due to the emigration of wandering cells towards the peripheral zone, creating a space and thus this presents a corona-like appearance specific to this type of growth. 3. As for cells wandering out, the majority of them are neutrophils at the early stage of culture,and gradually numbers of eosinophils and lymphocytes increase; and after 12 hours' culture monocytes make their appearance. 4. As regards the distribution of cells in the growth area after 24 hours' culture, the peripheral zone is occupied mainly by neutrophils, and eosinopbils are scattered there, too; and the intermediate zone is chiefly occupied by lymphocytes and a few monocytes, but the central part is occupied by many monocytes.
en-copyright=
kn-copyright=

en-aut-name=SogawaTamotsu
en-aut-sei=Sogawa
en-aut-mei=Tamotsu
kn-aut-name=十川保
kn-aut-sei=十川
kn-aut-mei=保
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=11
article-no=
start-page=4011
end-page=4023
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Bone-Marrow Tissue Culture, the Simple Method of one Design Part 2. The Bone Marrow of Patients with Various Blood Diseases
kn-title=簡易骨髄組織培養法に関する研究 第二編 各種血液疾患々者骨髄に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By performing a series of bone-marrow tissue culture of patients with various blood diseases by simple method the author studied the tissue growht and the wandering velocity of mature neutrophils, and arrived at the following conclusions: 1. In the case of chronic myelogenous leukemia the tissue growth is excellent, but the wandering velocity of mature neutrophils is somewhat decreased. In the cases of acute myelogenous leukemia monocytic leukemia the rate of relative growht is somewhat lower than in the case of normal persons: and the wandering velocity of mature neutrophils shows a markedly low value. The boundary of the growth zone is sharply demarcated, and the cell density index is high, presenting the growth pattern chareteristic to leukemia. 2. In the case of hypoplastic anemia the tissue growth is extremely poor; and the wandering velocity of mature neutrophils is markedly low, showing an increase of fat cells in the explant. 3. In the case of Banti's disease the tissue growth is rather poor, and the wandering velocity of mature meutrophils is low. 4. In the case of essential hypochromic anemia both the tissue growth rate and the wandering velocity of mature neutrophils are identical with those of normal persons. 5. In the case of agranulocytosis the tissue growth is extremely poor, and showing no increase of fat cells in the explant, the treatment with ACTH, however, improves the bone marrow function strikingly. 6. In the case of pernicious anemia the wandering velociyt of mature neutrophils is accelerted by the presence of vitamin B(12) in the medium. These findings by this method of culture coincide almost exactly with those by the conventional method, and it is believed that this method can be widely used in clinics due to its simplicity manipulation.
en-copyright=
kn-copyright=

en-aut-name=OnoYasuzo
en-aut-sei=Ono
en-aut-mei=Yasuzo
kn-aut-name=小野安三
kn-aut-sei=小野
kn-aut-mei=安三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=11
article-no=
start-page=4003
end-page=4010
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studdies on the Bone-Marrow Tissue Culture, the Simple Method of our Design Part 1. The Methodology and the Bone Marrow of Healthy Persons
kn-title=簡易骨髄組織培養法に関する研究 第一編 方法論並びに健康人骨髄に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Although several noteworthy data have been obtained by clinical application of bonemarrow tissue culture, in view of the complexity of its procedures we have devised a simple method of bone-marrow tissue culture modified from the cover-slip method for the purpose of a still wider clincal application. By observing the bone marrow of the sternum of healthy persons by means of this method, the following results were obtained: 1. The medium was consisted of a drop each of normal human serum and vitamin B(12) solution placed on a tissue culture plate of our own device, and the best results were obtained by the vitamin B(12) solution (containing 100 r/cc) to be added to the serum; and the most suitable space-distance between the surface of the slideglass and the coverglass was in the range of 150-200 μ. 2. In the determination of the relative growth rate, cell-density index and the wandering velocity of mature neutrophils in the bonemarrow tissue culture of the sternum of healthy persons, within 24 hours of the culture the results obtained resemble quite closely to those obtained by the conventional method of tissue culture, proving that this method is sufficiently worthy of clinical application even though the shortening in the duration of arresttime in all cells can be observed.
en-copyright=
kn-copyright=

en-aut-name=OnoYasuzo
en-aut-sei=Ono
en-aut-mei=Yasuzo
kn-aut-name=小野安三
kn-aut-sei=小野
kn-aut-mei=安三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=10
article-no=
start-page=3889
end-page=3903
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581031
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Leucocyte Function in Pulmonary Tuberculosis Part 3. The Effect of Thoraco-Surgical Treatment on the Leucocyte Function in Pulmonary Tuberculosis
kn-title=肺結核患者の白血球機能に関する研究 第三編 肺結核患者の白血球機能に及ぼす胸部外科手術の影響に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to see the effects of thoraco-surgical treatment on leucocyte function in pulmonary tuberculosis, the author examined the picture, wandering velocity, and phagocytesis of leucocytes in the patients with pulmonary tuberculosis, starting before operation and continued for three months; and obtained the following results; Pulmonary Resection 1. The total leucocyte count: The effect of surgical treatment is most marked on the day of operation, namely the leucocyte count increases up to 10,000-20,000, but rapidly decreasing thereafter, it returns to the pre-operation level by the 20th day and it keeps its normal level thereon. And the increase or decrease in the leucocyte count more or less parallels with the extent of operation and the increase or decrease in the neutrophil count. 2. Fluctuations of various leucocytes: The change in the neutrophil count takes about the same course as that of the leucocyte total. The eosinophil count begins to disappear immediately after operation and appear on the third day; thereafter, it returns to the pre-opsration level. As for the basophil count, with the exception of a transient increase on the third day, it shows no fixed trend neither monocytes. As for the percentage of lymphocytes, a marked decrease can be observed on the day of operation, and thereafter, recovering gradually, it takes somewhat the opposite course to that taken by neutrophils, but the absolute count shows no marked change. 3. The leucocyte function: The wandering velocity of leucocytes is accelerated on the day of operation, and declining thereafter, shows its minimum on the third day after operation, but by the 10th day it returns to the preoperation level, thereon it surpasses that level. The phagocytosis of leucocytes is promoted on the day of operation, and falling down to its minimum on the second day after operation, it returns to the preoperation level on the fifth day, and later it surpasses that level. Namely, on the day of operation leucocytes show what Sugiyama calls "progressive left-shift of the nucleus", and the second day as the turning point their picture is transferred to "regressive left-shift of the nucleus", and it returns to that before operation on the 5th to 10th day. Thereafter the leucocyte function is accelerated, and also it is obvious that the phagocytosis of leucocytes is more responsive to reaction than the wandering velocity Thoracoplasty Leucocyte findings and their function by this treatment show rather similar tendency observed in the Pulmonary resection. The improvement up to the 20th day after the treatment in this case, however, is relatively more rapid than that in the Pulmonary resection, while after one month the improvement is a little inferior to that by the former method. Group Showing Postoperative Complication About the 5th day after operation the decrease in the leucocyte count of this group is less than that of the group with good postoperative improvement, and also the recovery of the leucocyte function is slower, showing no return to the preoperation level. Summarizing the above, the thoraco-surgical treatment essentailly acts as a stress on patient, and the response to the operation appearing most markedly on the day of operation, the direct influence of operation disappears by the 20th day. Moreover, the group with postoperative complication the recovery of leucocyte findings and their function is prolonged. It is believed that the follow-up examinations of the leucocytes and the leucocyte function offer some important clues for the determination of postoperative conditions.
en-copyright=
kn-copyright=

en-aut-name=HaraMasao
en-aut-sei=Hara
en-aut-mei=Masao
kn-aut-name=原正夫
kn-aut-sei=原
kn-aut-mei=正夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=10
article-no=
start-page=3879
end-page=3888
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=1958
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Leucocyte Function in Pulmonary Tuberculosis Part 2. The Effects of Combination Therapy of Various Medicaments on the Leucocyte Function in Pulmonary Tuberculosis
kn-title=肺結核患者の白血球機能に関する研究 第二編 肺結核患者の白血球機能に及ぼす各種薬剤併用療法の影響に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the successive administration of combination of such drugs as SM-PAS, PAS-INH, SM-PAS-INH, and PZA-INH to 23 patients with pulmonary tuberculosis, the author investigated the effects of these combination therapies on the leucocyte count and on the neutrophil function in the course of treatment, and obtained the following results: 1. Leucocyte count: The leucocyte count decreases up to the 5 th clay in the case of SM-PAS-INH combination; up to the 10 th day in the SM-PAS or PZA-INH combination; and up to the 20 th day in the PAS-INH combination, and thereafter it gradually increases in everyone of these combinations, until finally losing the effectiveness of the combination drug therapy by the end of one-month treatment. There on the leucocyte count is controlled by the conditions of disease. 2. Various Icucocytes: In every combination neutrophils decrease up to the 5 th-20 th day; monocytes up to the 10 th-20 th day; and lymphocytes up to the 5 th-10 th day, but in eosinophils only an increase has been observed, After one month various leucocytes change in number according to the changes in the condition of disease itself rather than to the effect of drugs. 3. Leucocyte function: The influence of each combination therapy appears most effectively between the 10 th and 20 th days; and in the combinations of Styl-PAS and PAS-INH marked acceleration of the function is brough about; while the combinations of SM-PAS-INH and PZA-INH acts inhibitorily on the function. One month after the initiation of the combination treatment and thereafter the function is influenced secondarily by changes in the conditions of a patient due to drugs administered rather than by the effect of drugs itself. 4. In other words, admitting marked clinical effects of the combination thbrapy of anti-tuberculous agents, it is important to bear in mind a possibility of some unfavorable effects of the combination therapy on the leucocyte function.
en-copyright=
kn-copyright=

en-aut-name=HaraMasao
en-aut-sei=Hara
en-aut-mei=Masao
kn-aut-name=原正夫
kn-aut-sei=原
kn-aut-mei=正夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=10
article-no=
start-page=3869
end-page=3878
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581031
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Leucocyte Function in Pulmonary Tuberculosis Part 1. The Leucocyte Function and Conditions of Disease in Pulmonary Tuberculosis
kn-title=肺結核患者の白血球機能に関する研究 第一編 肺結核患者の白血球機能と病状に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=After the general examination of blood and examinations of the leucocyte function and the pattern of their movement in 48 patients with pulmonary tuberculosis, the author studied the relationship between the results of examinations and conditions of disease; and obtained the following results: 1. The leucocyte count inceases along with aggravation of disease; and there can be observed a marked increase of lymphocytes in minimal cases; of monocytes, neutrophils and lymphocytes in moderately advanced cases; and of monocytes and neutrophils in far advanced cases. In other words, the increase in monocytes and neutrophils becomes marked as tuberculosis advances in severity. On the other hand, the percentage of lymphocytes indicates the advance of tuberculosis. Moreover, the farther advanced the disease, the more marked is the tendency of the left-shift of the nucleus in neutrophils, and also the ratio L: M decreases accordingly. Although a decrease in eosinophils can generally be observed, there is no di stinct correlation between this decrease and the condition of disease. 2. Leucocyte function: The leucocyte function in minimal cases generally is about the same as that of the normal persons, but as the disease advances to moderately advanced and to far advanced stages, it decreases along with the advance. The leucocyte function declines in proportion to the increase in erythrocyte sedimentaion rate, and those with over 25 mm erythrocyte sedimentation rate show a marked decline in the function. According to Oka's classification based on X-ray findings, the function in Type �Z is markedly lower than that in Types �Y, �W and �X. And according to the NTA classification as the extensiveness of lesions advances from the minimal to moderately advanced and far advanced, or according to the clinical classification as the inactive type moves to the arrest and to the active typse, the leucocyte function proportionately decreases more and more markedly. Relative to number of tuberculous bacilli, in those who are negative to bacillus culture the leucocyte function does not differ much from that of the normal, but as the culture, bacillus collection and smear specimens turn to positive, the leucocyte function gradually declines. As for the relationship with the body temperature and pulses, along with the rise in body temperature and increase in pulse-beats the leucocyte function diminishes, especially the decline is quite marked in the case with the body temperature of over 38℃ and pulses over 100. Moreover, in the case when the leucocyte count increases, the leucocyte function is enhanced up to the count of 8,000, but beyond 10,000 the function is extremely diminis hed. The greater the average number of nuclei, the greater in the acceleration of leucocyte function, and in those whose L/M ratio is 16-23, the leucocyte function is highest. Of those patients with pulmonary tuberculosis of moderately advanced stage or far advanced showing an increase in leucocyte count, a decrease in the average nucleus count, and a low L/M ratio; the ones with accelerated leucocyte function later improve, while ones with lowered leucocyte function still get worse and worse. 3. Movement pattern of leucocytes in pulmonary tuberculosis: As the disease aggravates, Types A(3), B(2), and D with low wandering velocity increase in number. In addition, even in those with the same movement pattern, as the disease aggravates, the wandering velocity decreases that much more.
en-copyright=
kn-copyright=

en-aut-name=HaraMasao
en-aut-sei=Hara
en-aut-mei=Masao
kn-aut-name=原正夫
kn-aut-sei=原
kn-aut-mei=正夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=8
article-no=
start-page=2773
end-page=2781
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Monocytic Leukemia by means of Bone-MarrowTissue Culture Part 2. Studies on Cell Function of Bone Marrow by Wandering Velocity, Carbon- Particle Phagocytosis, and Neutral- Red Vital Staining
kn-title=骨髄体外組織培養による単球性白血病の研究 第2編 遊走速度,墨粒貪喰能, 中性紅生体染色性による骨髄の細胞機能に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By means of borne-marrow tissue culture, the author has investigated the wandering velocity, phagocytosis of carbon particles and neutral-red vital staining of neutrophils and monocytes in both patients and normal persons, and obtained the following results. 1. The wandering velocity of the neutrophils and monocytes in patients was lower than that in normal persons, and the span of cell-wandering in th former was shortened. 2. Phagocytosis of carbon particles of the neutrophils and monocytes in patients was decreased in comparison with that of the control. The time required for the phagocytosis of neutrophils in tissue culture to reach its peak is longer than that in the case of the control. The phagocytosis of the monocytes in patients gradually increased, making a similar curve as that of the control but a lower one, and both reached their peak in 24 hours. The peak of the former, however, was markedly lower. In addition, promonocytes occasionally showed very slight phagocytosis of carbon particles in the cases whose cell functions were not so markedly decreased. 3. As for the neutral-red vital staining the nutrophils and monocytes in patients were stained much more rapidly and more deeply but they fade very quickly as compared with those of the control. 4. From the above mentioned results, the author has confirmed the decrease in the cell functions of the bone marrow in monocytic leukemia.
en-copyright=
kn-copyright=

en-aut-name=KawanoYoshihiko
en-aut-sei=Kawano
en-aut-mei=Yoshihiko
kn-aut-name=川野嘉彦
kn-aut-sei=川野
kn-aut-mei=嘉彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=7
article-no=
start-page=2649
end-page=2660
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Vital Staining in the Bone-Marrow Tissue Culture Part 3. Clinical Application of Vital Staining in Bone-Marrow Tissue Culture
kn-title=骨髄体外組織培養に於ける生体染色の研究 第3編 臨床的応用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By adding the serum of patients with varicus blocd diseases to the medium of bone-marrow tissue culture of rabbits, the author studied mainly its influence on the vital staining of pseudoeosinophils with neutral red. At the same time the author pursued vital staining of neutrophils in the bone marrow of the sternum from the same patients with neutral red; and as for the verification of the study, the cells that had been cultured for several hours were vitally stained. As the result the author arrived at the following conclusions: 1. When the cell functions (wandering velocity and carbon-particle phagocytosis) are low during the tissue culture, vital staining by neutral red takes place fast and highly but the stained color fades quickly. 2. Also when the vital staining is done after fixed intervals of culture, the longer the time of culture is, namely, at the time when the cell functions are further diminished, the more prompt and the higher is the staining and also the fading takes place more quickly. 3. Those patients showing lowered cell functions on the vital staining of bone marrow are aplastic anemia, Banti's disease, leukemia, acute bleeding anemia and kala-azar. 4. Likewise those who show a slight lowering in the cell functions are ancylostomiasis, essential hypochromic anemia and Werlhof's disease; and those whose functions are hardly lowered are those patients suffering from lung tuberculosis and malignant nephrosclerosis, namely, not from blood diseasses. 5. On vital staining the sera that possess factors of lowering the functions of bone marrow are those of aplastic anemia, Banti's disease, acute bleeding anemia, Werlhof's disease, and leukemia.
en-copyright=
kn-copyright=

en-aut-name=TamuraHajime
en-aut-sei=Tamura
en-aut-mei=Hajime
kn-aut-name=田村甫
kn-aut-sei=田村
kn-aut-mei=甫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=7
article-no=
start-page=2641
end-page=2647
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Vital Stainings in the Bone-Marrow Tissue Culture Part 2. On Vital Staining of Various Leucocytes in Bone-Marrow of Normal Rabbits and Normal Persons
kn-title=骨髄体外組織培養に於ける生体染色の研究 第2編 健康家兎並びに健康人骨髄内各種白血球の生体染色に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the bone-marrow tissue culture of normal rabbits and normal persons, by performing vital staining of tissues with neutral red and lithium carmine, the author in vestigated the degree of staining in various bone marrow cells appearing in the growth zone of explant, and obtained the following conclusions: 1. Neutral red stains much more markedly than lithium carmine, and it has been found that neutral red is quite suitable for vital staining in observing bone marrow cells. 2. The stainability of pseudoeosinophils in the bone marrow in normal rabbits is far clearer than that of human neutrophils. 3. The stainability of each of various bone marrow cells appearing in the growth zone in bone-marrow tissue culture of normal rabbit reveals such a peculiarity that it make the differentiation of cells quite easy. 4. Investigating the degrees of staining by neutral red of pseudoeosinophils in rabbit bone marrow from the standpoint of time element, it has been found that staining of cells commences immediately after addition of dyes to the medium, reaching the maximum staining after about 5 hours' culture, but thereafter the stained color begins to fade gradually, and within 24-30 hours it loses its color completely.
en-copyright=
kn-copyright=

en-aut-name=TamuraHajime
en-aut-sei=Tamura
en-aut-mei=Hajime
kn-aut-name=田村甫
kn-aut-sei=田村
kn-aut-mei=甫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=7
article-no=
start-page=2591
end-page=2598
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Anemia in Anchylosot. miasis by means of Bone Marrow Tissue Culture Part 3. Influences of an Addition of the Serum of Hook Worm Dogs on Bone Marrow Tissue Culture
kn-title=骨髄組織培養による鉤虫症貧血の本態に関する研究 第3編 鉤虫犬血清添加が同種骨髄組織培養に及ぼす影響 附 全編の総括
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By adding a drop of the serum of dogs with ancylostomiasis in the medium of bone marrow tissue culture of normal dogs, the author has examined its influences on the growth rate, white blood cell function (wandering velocity, phagocytosis of carbon particles and vital staining of neutral red) in hanging drop culture and increasing rate of the red blood cell count and hemoglobin content in fluid culture. 1) As for the growth rate, it was slightly increased in the later atage of tissue culture. 2) Function of the neutrophils was not impedented on wandering velocity, phagocytosis of carbon particles and vital staining of neutral red, but rather slightly accerelated in the early stage of tissue culture. 3) According to the above mentioned results, the author has presumed that some stimulative substances for the bone marrow activity and neutrophils function are contained in the serum of hook wormdog. 4) Wandering velocity of the eosinophils was slightly increased, so that the author has resumed that it had occured from the stimulation of hook worm toxin possessing positive taxis for the eosinophils. 5) Increasing rate of the red blood cell count was not suffered from by adding the serum of hook worm dog on fluid culture, but that of hemoglobin content was slightly declined, which did not come from the restrain of hook worm toxin for the formation of hemoglobin, but from iron deficiency in the serum.
en-copyright=
kn-copyright=

en-aut-name=YumotoMasaru
en-aut-sei=Yumoto
en-aut-mei=Masaru
kn-aut-name=柚本賢
kn-aut-sei=柚本
kn-aut-mei=賢
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=7
article-no=
start-page=2581
end-page=2590
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Anemia in Anchylostomiasis by means of Bone Marrow Tissue Culture Part 2. Bone Marrow Tissue Culture of Dogs with Anchylostomiasis
kn-title=骨髄組織培養による鉤虫症貧血の本態に関する研究 第2編 実験的鉤虫犬骨髄組織培養に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By means of bone marrow tissue culture, the author has investigated the hematopoietic activity and blood cell function in the bone marrow of the dogs experimentally infected with ca. 1000 hook worm larvae, which were classified into 3 groups of initial slight, moderate and severe anemia according to the level of anemia. 1) In a group of initial slight anemia, the growth rate was increased and the function of the neutrophils was accelerated in wandering velocity, phagocytosis of carbon particles and vital staining of neutral red. Wandering velocity of the eosinophils in the bone marrow was markedly accelerated. Increasing rate of the red blood cell count and hemoglobin content in fluid culture was slightly lowered. 2) In a group of moderate anemia with profuse intestinal bleeding, the growth rate was further increased, and wandering velocity was still kept in a high degree, but their function on phagocytosis of carbon particles and vital staining of neutral red was fairly lowered. Namely dissociation of the functions of neutrophils was observed in this group. Wandering velocity of the eosinophils was still elevated. Increasing rate of the red blood cell count was slightly elevated in comparison with that of the control one, but that of hemoglobin content being fairly lowered. 3) In a group of severe anemia with profuse intestinal bleeding and seriuos symptoms, the growth rate was most increased, wandering velocity being still kept nearly in the same level as that of the control one. But their function on phagocytosis of carbon particles and vital staining of neutral red was remarkably lowered. Wandering velocity of the eosinophils was reveresely lowered in proportion to the decrease of the eosinophils in peripheral blood. Increasing rate both of the red blood cell count and hemoglobin content was most lowered. 4) According to the above mentioned results, the author has confirmed that anemia in a group of initial slight anemia without intestinal bleeding was caused from blood cell arrest in the sinusoid of the bone marrow and disturbance of iron mobilization by hook worm toxin, but in groups of moderate and severe anemia with profuse intestinal bleeding, influences due to bleeding took a great role for the development of anemia besides the above mentioned mechanismus.
en-copyright=
kn-copyright=

en-aut-name=YumotoMasaru
en-aut-sei=Yumoto
en-aut-mei=Masaru
kn-aut-name=柚本賢
kn-aut-sei=柚本
kn-aut-mei=賢
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=7
article-no=
start-page=2569
end-page=2579
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Anemia in Anchylostomiasis by means of Bone Marrow Tissue Culture Part 1. Bone Marrow Tissue Culture of Patients of Anchylostomiasis
kn-title=骨髄組織培養による鉤虫症貧血の本態に関する研究 第1編 鉤虫症患者骨髄組織培養に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By means of bone marrow tissue culture devised in author's laboratory, the author has investigated the hematopoietic activity and white blood cell function (wandering velocity, phagocytosis of carbon particles and vital staining) in the bone marrow of 16 cases of patients of anchylostomiasis, which were classified into 3 groups according to the level of anemia. 1) Relative growth rate in hanging-drop culture was hardly lowered in most cases, but in a group of severe anemia with a slight decrease of the white blood cell count in peripheral blood, it being slightly lowered. 2) Wandering velocity of the neutrophils was hardly decreased in most cases, but in a group of severe anemia with a slight decrease of the whitebolod cell count, it being slightly decreased. 3) Phagocytosis of carbon particles of the neutrophils was hardly declined in a group of slight anemia, but along with a development of anemia in peripheral blood, it being fairly declined, especially in a group of severe anemia with a slight decrease of the white blood cell count. 4) As for the vital staining of neutral red, it was little altered in most cases, but in cases of severe anemia with a slight decrease of the white blood cell count, the neutrophils were stained relatively in the early stage and high degree, and discolored comparatively earlier than those of the control one. 5) Wandering velocity of the eosiuophils in the bone marrow was markedly accelerated in most cases with eosinophilia in peripheral blood, sometimes being more than 2 times those of the control one. 6) Increasing rate of the red blood cell count and hemoglobin content in fluid culture were slightly decreased, especially in groups of moderate and severe anemia. 7) According to the above-mentioned results, the author has confirmed that the hematopoietic activity and blood cell function in the bone marrow of anchylostomiasis were scarecely deteriorated and the development of anemia should be regarded as the results of blood cell arrest in the sinusoid of the bone marrow and deficiency of an iron supply to the hematopoietic tissues due to the disturbance of iron mobilization, and moreover the development of hypoplastic anemia in anchylostomiasis should be considered as a secondary occurrence following a long durated severe anemia.
en-copyright=
kn-copyright=

en-aut-name=YumotoMasaru
en-aut-sei=Yumoto
en-aut-mei=Masaru
kn-aut-name=柚本賢
kn-aut-sei=柚本
kn-aut-mei=賢
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=7
article-no=
start-page=2517
end-page=2524
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Eosinophils in the Bone-Marrow Tissue Culture Part 2. Observation on the Motility of Eosinophils in the Bone-Marrow Tissue Culture of the Sternum of various Patients with Eosinophilia
kn-title=骨髓体外組織培養による好酸球に関する研究 第2編 各種好酸球増多症患者の胸骨骨髓培養における好酸球運動の観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By performing bone-marrow tissue culture (simple method devised in our laboratory) of the sternum in normal person, patients wish anchylostomiasis and with bronchial asthma, the author observed the pattern of movement and wandering velocity of these eosinophils and obtained the following results: 1. Patterns of movement of eosinophils in the human bone marrow have been newly classified. 2. Among the eosinophils of human anchylostomiasis there are ones that show a peculiar pattern of movement, Type IB, which may be considered to be the acceleration in the ability of retraction of the exoplasm. In addition, a picture of scattered granules can often be recognized. The wandering velocity of eosinophils in this disease is markedly accelerated, showing the veloeity somewhat superior to that of neutrophils. 3. In the eosinophils of bronchial asthma there are cells suggesting an abnormally accelerated adhesive property, for example, possessing abnormally distended tail or filamentous tail with many branches; and also granule-movement is quite active. As can be seen from the above findings, there are eosinophils in anchylostomiasis and bronchial asthma, that show the pattern of movement that can not be observed in those of normal persons, and therefore, it has been clarified that those eosinophils of the bone marrow in patient not only multiply in number but also their pattern of movement undergoes morphological changes simultaneously.
en-copyright=
kn-copyright=

en-aut-name=InoueMasakatsu
en-aut-sei=Inoue
en-aut-mei=Masakatsu
kn-aut-name=井上正勝
kn-aut-sei=井上
kn-aut-mei=正勝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=10-12
article-no=
start-page=857
end-page=867
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1961
dt-pub=19611230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Treatment of Leukemia and Clinical Bone Marrow Tissue Culture Part 2. A Study on Cytomorphological Changes of Bone Marrow Cells by Vital Staining
kn-title=白血病の治療と臨床骨髄組織培養 第2編 生体染色による骨髄細胞の細胞学的変化に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The blood cells in the bone marrow of leukemia before and after antileukemic chemotherapy and those of hypoplastic anemia were observed morphologically by vital staining. 1. In acute myelogenous leukemia at remission, the mitochondria of the atypical promyelocytes displayed variation in size and those of the lymphocytes and the erythroblasts showed the same change. Some of neutral red granules of the young neutrophilic cells became larger in size. 2. In acute lymphocytic leuklemia at remission, the mitochondria of the lymphoblasts and the atypical lymphocytes exhibited a considerable variation in size and during the tissue culture, some of those mitochondria developed small vacuoles. 3. In monocytic leukemia at remission, the mitochondria of the promonocytes displayed a slight variation in size. 4. In chronic myelogenous at remission, the mitochondria of the young neutrophilic blood cells became varying in size and many neutral red vacuoles appeared in the cytoplasm of mature neutrophils. Appearance of many unstainable vacuoles around the nucleus of the myelocytes seemed to be characteristic in the chronic myelogenous leukemia at remission that showed the hypolastic pattern in bone marrow tissue culture. 5. In hypoplastic anemia, unstainable vacuoles which were observed in treated chronic mylogenous leukemia made no appearance. In conclusion, the morphologic changes observed after treatment were considered to be the pictures of cellular degeneration that were superimposed over the cytologic characteristics of leukemic cells and accordingly, by this feature leukemia was able to be cytologically diagnosed even at remission.
en-copyright=
kn-copyright=

en-aut-name=KawaharaToru
en-aut-sei=Kawahara
en-aut-mei=Toru
kn-aut-name=河原徹
kn-aut-sei=河原
kn-aut-mei=徹
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=4
article-no=
start-page=1315
end-page=1329
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Tissue Culture of the Bone Marrow in Various Animals Part 3. Vital Staining of Bone Marrow Cells
kn-title=各種動物の骨髄体外組織培養 第3編 骨髄細胞の生体染色に就て 附,全編の総括
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With bone-marrow tissue culture of various animals, namely, mammals (mice, albino rats, guinea pigs, cats and dogs), birds (chicken and pigeons), and cold blooded animals (frogs), the author studied neutral red vital staining of bone marrow cells and arrived at the following conclusions. 1) In the neutrophilic series, granules of young immature cells have a strong affinity to neutral red, and mature neutrophils are stained in intermediate degree. The stained granules tend to fuse with one another and grow larger alog with the lapse of time. 2) Practically all eosinophils are stained light red, while basophilocytes are all stained deep red. Eosinophils of birds show some reluctancy to be stained. Monocytes on the whole are stained in intermediate degree and they are usually arranged in the rosette formation, and those in birds and frogs are arranged in a typical fashion, Lymphocytes, megakaryocytss thrombocytes and spindle cells all clearly show neutral red granules. 3) As for the stainability of neutrophils and pseudo-eosinophils with respect to the passing of time in birds, it is generally highest in pseudo-eosinophils and also the average rate of staining is higher than that of mammals, but their color fades more quickly. In neutrophils of frogs the rate of this color-fading is rather slower than that of warm-blooded animals.
en-copyright=
kn-copyright=

en-aut-name=SuenagaTaizo
en-aut-sei=Suenaga
en-aut-mei=Taizo
kn-aut-name=末永泰三
kn-aut-sei=末永
kn-aut-mei=泰三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=4
article-no=
start-page=1303
end-page=1314
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Tissue Culture of the Bone Marrow in Various Animals Part 2.Carbon-Particle Phagocytosis of Bone Marrow Cells
kn-title=各種動物の骨髄体外組織培養 第2編 骨髄細胞の墨粒貪喰能に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With bone-marrow tissue culture of such various animals as mammals (mice, albino rats, guinea pigs, cats and dogs), birds (chicken and pigeons) and cold blooded animis (frogs), the author studied the phagocytosis of carbon particles by bone-marrow cells and obtained the following conclusions. 1) In the neutrophilic series myeloblasts, promyelocytes and myelocytes show no phagocytic power. Metamyelocytes of mammals show a sligh phagocytic capacily; but it is wanting in the species lower than birds. On the other hand, mature neutrophils show a considerable phagocytic power. 2) Eosinophils, basophilocytes and lymphocytes have no phagocytic capacity nor megakaryocytes, but thrombocytes possess adhesive and phagocytic abilities. A slight ingestion of carbon particles by spindle cells can be observed in the species lower than birds. 3) Comparing the average rate of phagocytosis of mature neutophils, it is generally higher in mammals, followed by that of frogs, and it is lowest in pseudo-eosinophils of birds. 4) The phagocytic ability of monocytes increases along with the lapse of time, surpassing that of neutrophils, and it does not decrease even with the passing of time. Moreover, the average rate of phagocytosis of monoytes shows no significant difference among various animals as observable in the case of neutrophils.
en-copyright=
kn-copyright=

en-aut-name=SuenagaTaizo
en-aut-sei=Suenaga
en-aut-mei=Taizo
kn-aut-name=末永泰三
kn-aut-sei=末永
kn-aut-mei=泰三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=4
article-no=
start-page=1291
end-page=1302
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Tissue Culture of the Bone Marrow in Various Animals Part 1. Observations on Tissue Growth and Wandering Cells
kn-title=各種動物の骨髄体外組織培養 第1編 組織増生並びに遊走細胞の観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By conducting a series of tissue culture with bone marrow obtained from mammals (mice, albino rats, guinea pigs, cats and dogs), birds (chicken and pigeons), and cold blooded animals (frogs) the author studies the rate of tissue growth and wandering cells, and obtained the following results. 1) As for the growth area, the explant in culture grows up to 72 hours in the cases of albino rats, guinea pigs and cats, while in the case of other animals the growth continues almost up to 48 hours. However, in the case of mature dogs the growth ceases within 24 hours, and the relative growth in the cases of mature dogs and frogs presents a marked decrease. 2) In comparing the index of the cell density at the 24-hour culture, it is markedly lower in the cases of dogs and frogs, whereas in other animals it is over 50. 3) Myeloblasts, promyelocytes and myelecytes of all these animals have no active wandering capacity, and at the stages of maturation later than metamyelocytes cells show the wandering capacity. Neutrophils and pseudoeosinophils show the most active pseudopoid movement. 4) As for the wandering capacity of basophilocytes, the lower the species of animals the poorer is their wandering capacity. However, as for the wandering capacity of eosinophils, lymphocytes and monocytes no marked difference in the wandering capacity can be observed among different animals. Motility of thrombocytes can be observed and moreover, even in species lower than birds, a slight pseudopoid movement of spindle cells can be recognized. 5) Comparing the wandering velocity of neutrophils and pseudo-eosinophils of various animals, with exception of dogs that show generally a decrease, no marked difference in the maximum wandering velocity among them. As for the duration of wandering, with one or two exceptions, generally the lower the species the shorter is the duration. 6) In comparing various plasma such as those of dog, man, rabbit and chicken as the medium for bone marrow culture of the dog, dog plasma gives the best relative-growth value as well as the highest wandering velocity of neutrophils.
en-copyright=
kn-copyright=

en-aut-name=SuenagaTaizo
en-aut-sei=Suenaga
en-aut-mei=Taizo
kn-aut-name=末永泰三
kn-aut-sei=末永
kn-aut-mei=泰三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=7-9
article-no=
start-page=589
end-page=593
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1961
dt-pub=19610930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Phagocytosis of Leucocytes, especially of Neutrophils, for Carbon Particles in Acatalasemia and Various Oto-rhino-laryngological Patients
kn-title=無カタラーゼ血液症及び諸種耳鼻咽喉科疾患々者に於ける白血球（特に好中球）の墨粒貪喰能に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Phagocytosis of leucocytes in the blood of acatalasemia for carbon particles was investigated by means of the slide method, using the blood of healthy people and various oto-rhino-laryngological patients as controls. Phagccytosis in neutrophils was classified into five degrees from negative to 3 positives (-, ±, +, ++, +++), giving them values of 0, 1, 2, 3 and 4 respectively, according to the degrees of phagocytosis. The mean values counted by this method were as follows; 1) Number of healthy people examined was seven and the mean value was 2.55. 2) Nineteen otorhinolaryngological patients were examined and their mean value proved to be 2.50. The mean value of thirteen out of these nineteen patients was 2.54, except six cancer patients. Three out of these six cancer patients had been treated with radium and the mean value did not differ from that of healthy people, while three X-ray treated patients showed the mean value of 2.31. 3) Number of acatalasemia patients was two and their mean value 2.53. As far as the present experiment is concerned, there appears no significant difference in the degree of phagocytosis in leucocytes for carbon particles in the groups examined except X-ray treated cancer patient group, who showed lower mean value.
en-copyright=
kn-copyright=

en-aut-name=OritaYozo
en-aut-sei=Orita
en-aut-mei=Yozo
kn-aut-name=折田洋造
kn-aut-sei=折田
kn-aut-mei=洋造
aut-affil-num=1
ORCID=

en-aut-name=TamuraMinoru
en-aut-sei=Tamura
en-aut-mei=Minoru
kn-aut-name=田村実
kn-aut-sei=田村
kn-aut-mei=実
aut-affil-num=2
ORCID=

en-aut-name=MoritaYoshio
en-aut-sei=Morita
en-aut-mei=Yoshio
kn-aut-name=森田善雄
kn-aut-sei=森田
kn-aut-mei=善雄
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部耳鼻咽喉科学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部耳鼻咽喉科学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部耳鼻咽喉科学教室
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=7-9
article-no=
start-page=553
end-page=578
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1961
dt-pub=19610930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental studies on the effect of exhaustive physical exercise against radiation injury. Part 1
kn-title=運動負荷が放射線障害に及ぼす影響に関する実験的研究 第1編 放射線照射後の連続的運動負荷が生存率,体重変化及び血液諸成分に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Guinea pigs were divided into the next four groups for the purpose of examining the effects of exhaustive physical exercise and fatigue after exposing to X-ray. (1) Non treated group (C) (2) Exercised control group (EC) (3) Radiated control group (RC) (4) Radiated exercised group (RE) The X-ray apparatus was operated at. 200 KV, 15 mA, 0.5 mm Copper and 0.5 mm Alminium filtration, 50 cm focus target distance. The dose was 200 r of. X-ray whole body irradiation in dose rate of 80.6 r/min (in the air). Subjects of this experiment were given 30 successive days of swimming at the rate of 30 minutes per day and 5 days per week. The results are as follows: (1) The 30 days survival rate of RE was lower than that of RC. (2) The decreasing rate of the body weight in RE was a little higher than that in RC. (3) The prolongation of the blood clotting time in RE was greater than that in RC. (4) The decreasing rate of red cell count in RE was a little higher than that in RC. In both groups the decreasing rate was the highest on the 14th day after the treatment. (5) The decreasing rate of hemoglobin content in RE was a little higher than that in RC. In both groups the decreasing rate was the highest on the 14th day after the treatment. (6) Reticulocyte count in RE and RC decreased in the beginning, but increased extremely on the 21st day after treatment. Its increasing and decrasing rate in RE was little different from that in RC. (7) The increasing rate of Heinz body in RE was higher than that in RC. (8) The decresing rate of white cell count in RE was not significantly different from that in RC. In both groups the white cell count was at minimum on the 14th day after treatment. (9) Concerning the classification of white cell, i.e. basophile., eosinophile-, neutrophile leucocyte, lymphocyte and monocyte, RE was not significantly different from RC. (10) About the changes of plasma proteins, a) The total protein in RC decreased a little in the beginning, but slightly increased in the latter period. That in RE showed little change in the beginning, and slightly increased in the latter period. Difference between RE and RC was not significant, b) A/G ratio did not change much in the beginning in RC as well as RE, but in the latter period it decreased in RC while increased in RE. c) Albumin didn't show much change in the beginning both in RC and RE, but it decreased a little in the latter period where as in RC it increased in RE. d) α-globulin in RC and RE increased a little after the treatment. Difference between RC and RE was not significant. e) β-globulin in RC decreased a little in the beginning, but increased in the latter period. On the contrary in RE, it decreased extremely in the latter period. f) Fibrinogen in RC and RE increased. Difference between RC and RE was not significant. g) γ-globulin n RC and RE decreased in the beginning. That in RC increased extremely, while in RE continued to decrease in the latter period.
en-copyright=
kn-copyright=

en-aut-name=OkahiraKazuma
en-aut-sei=Okahira
en-aut-mei=Kazuma
kn-aut-name=岡平和磨
kn-aut-sei=岡平
kn-aut-mei=和磨
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部衛生学教室
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=4-6
article-no=
start-page=387
end-page=398
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1961
dt-pub=19610630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Stndies on the Pneumoconiosis of Electric Welders
kn-title=電気熔接工のじん肺について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Sadatoshi, one of the authors, had reported previously that he had found 26% of electric welders at a certain heavy industrial plant suffered from pneumoconiosis by roentgenological examination. In this time investigation was done upon 78 electric welders carrying pneumoconiosis without pulmonary tuberculosis and the result was as follows: 1) Chest X-ray findings approved granulated type. No fibrosis, tendency of confluence, emphysema and eggshell calcification. Changes in hilus could not be found at the begining. 2) No visible changes in blood findings. Except, as the type advanced, a tendency of a slight decrease in neutrophil leukocytes and increase in lymphocytes could be seen. 3) No remarkable changes admitted in lung vital capacity and maximum ventulating volume. Expiratory impediment appeared in the third type. 4) Though welding dust contains SiO(2), welder's pneumoconiosis is innocent compared with silicosis. It also seems a little different from siderosis and so is suitable to classify it as "welder's Pneumoconiosis". 5) Occupational career should be cared when taking routine X-rayphotographs.
en-copyright=
kn-copyright=

en-aut-name=SadatoshiKurashi
en-aut-sei=Sadatoshi
en-aut-mei=Kurashi
kn-aut-name=貞利庫司
kn-aut-sei=貞利
kn-aut-mei=庫司
aut-affil-num=1
ORCID=

en-aut-name=AoyamaHideyasu
en-aut-sei=Aoyama
en-aut-mei=Hideyasu
kn-aut-name=青山英康
kn-aut-sei=青山
kn-aut-mei=英康
aut-affil-num=2
ORCID=

en-aut-name=ItamiYoshiaki
en-aut-sei=Itami
en-aut-mei=Yoshiaki
kn-aut-name=伊丹仁朗
kn-aut-sei=伊丹
kn-aut-mei=仁朗
aut-affil-num=3
ORCID=

en-aut-name=OnoMinoru
en-aut-sei=Ono
en-aut-mei=Minoru
kn-aut-name=大野稔
kn-aut-sei=大野
kn-aut-mei=稔
aut-affil-num=4
ORCID=

en-aut-name=OhnoKimiyoshi
en-aut-sei=Ohno
en-aut-mei=Kimiyoshi
kn-aut-name=小野公義
kn-aut-sei=小野
kn-aut-mei=公義
aut-affil-num=5
ORCID=

en-aut-name=OharaYasuro
en-aut-sei=Ohara
en-aut-mei=Yasuro
kn-aut-name=尾原安郎
kn-aut-sei=尾原
kn-aut-mei=安郎
aut-affil-num=6
ORCID=

en-aut-name=KatoShozi
en-aut-sei=Kato
en-aut-mei=Shozi
kn-aut-name=加藤尚司
kn-aut-sei=加藤
kn-aut-mei=尚司
aut-affil-num=7
ORCID=

en-aut-name=KanaiYoshiaki
en-aut-sei=Kanai
en-aut-mei=Yoshiaki
kn-aut-name=金井義明
kn-aut-sei=金井
kn-aut-mei=義明
aut-affil-num=8
ORCID=

en-aut-name=MitaniYasuo
en-aut-sei=Mitani
en-aut-mei=Yasuo
kn-aut-name=三谷恭夫
kn-aut-sei=三谷
kn-aut-mei=恭夫
aut-affil-num=9
ORCID=

en-aut-name=MotoiMakoto
en-aut-sei=Motoi
en-aut-mei=Makoto
kn-aut-name=元井信
kn-aut-sei=元井
kn-aut-mei=信
aut-affil-num=10
ORCID=

en-aut-name=MoriTakashi
en-aut-sei=Mori
en-aut-mei=Takashi
kn-aut-name=森巍
kn-aut-sei=森
kn-aut-mei=巍
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=玉野三井綜合病院

affil-num=2
en-affil=
kn-affil=岡山大学医学部衛生学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部

affil-num=4
en-affil=
kn-affil=岡山大学医学部

affil-num=5
en-affil=
kn-affil=岡山大学医学部

affil-num=6
en-affil=
kn-affil=岡山大学医学部

affil-num=7
en-affil=
kn-affil=岡山大学医学部

affil-num=8
en-affil=
kn-affil=岡山大学医学部

affil-num=9
en-affil=
kn-affil=岡山大学医学部

affil-num=10
en-affil=
kn-affil=岡山大学医学部

affil-num=11
en-affil=
kn-affil=岡山大学医学部
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=2
article-no=
start-page=499
end-page=511
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Studies on the Helminth Allergy Part �T. The Transitory Infiltration of the Lungs Caused by Ascaris Allergy
kn-title=寄生虫アレルギーに関する研究 第1編 蛔虫性アレルギーによる肺の一過性浸潤に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By oral administration of the eggs of ascaris to guinea pigs and rabbits, the animals were infected, or reinfected, or reinfested after sensitizing them with ascaris emulsion; and the blood picture. the roentgenograms of the lungs as well as histo-pathological findings of respective groups were comparatively studied. The results obtained are described below. 1) In blood picture ebsinophilia has been recognized in all the cases, whereas it is most marked in the cases where the animals are reinfested after the sensitization with ascaris emulsion. 2) When a large amount of ascaris eggs is given in the first administratios the shadows like pneumonia or nodular shadows are revealed in the roentgenograms of the lungs while in the cases of super infect or reinfection only, the appearance of these shadows is on the whole quicker and relatively more nodular shadows are observed as compared with the former. Of the four cases receiving the ascaris eggs after sensitization with the emulsion of ascaris, one case presented a picture of transitory infiltration in the lungs. 3) In the primary infection cases, hyperemia and hemorrhages are generally the main findings at early stage but later on the hypertrophy of alveolar septum can be recognized due to the infiltration of neutrophils, eosinophils, lymphocytes, and moncytes and the hypertrophy and proliferation of alveolar epithelium. In the cases reinfected or reinfected after the sensitization with the ascaris emulsion, the swelling and hypertrophy of blood-vessel wall and edemas in the perivascular space are more marked than the former, and likewise the nodular cell infiltration and the appeareance of giant cells are more striking than in the first infection cases. Grannloma formation can also be recognized in the latter. 4) From these findings it has been found that the ascaris allergy plays one of the pathogenetic roles in the transitory infiltration of the lungs.
en-copyright=
kn-copyright=

en-aut-name=IshidaToyosige
en-aut-sei=Ishida
en-aut-mei=Toyosige
kn-aut-name=石田豊重
kn-aut-sei=石田
kn-aut-mei=豊重
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=4-6
article-no=
start-page=339
end-page=351
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1961
dt-pub=19610630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Studies on the prophylactic Treatment of Cancer with Various Anticancer Agents Part �U. Influence of Anticancer Agents on the Inoculation of Brown-Pearce Carcinoma into Abdominal Wall of Rabbit, and Changes in Peripheral Blood Picture
kn-title=各種制癌剤による手術創の癌再発予防に関する実験的研究 第�U編 各種制癌剤のBrown-Pearce癌の腹壁筋内移植に及ぼす影響とその末梢血液像の変化について（実験的研究）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Immediately after inoculation of Brown-Pearce Carcinoma into the abdominal wall of rabbit, various anticancer agents (Nitromin, Carzinophilin, Azan, Tespamin and Mitomycin) were administered locally and their inhibitory effect for growth of tumor in loco with change in peripheral blood picture were observed. Results obtained were as follows: 1. Nitromin; no preventive effect on the inoculated carcinoma, Carzinophilin; most effective in agents tested, and inhibited the tumor growth in five out of ten animals, Azan; inhibited the tumor growth in only one out of ten, Tespamin; no effect like in Nitromin, Mitomycin; inhibited tumor growth in two out of ten. 2. In case of no tumor formation in the abdominal wall, leucemoid reaction, increases in monocytes and neutrophiles were not observed. In case of tumor formation, on the contrary, the reaction and the increases were remarkable.
en-copyright=
kn-copyright=

en-aut-name=SatoYasuo
en-aut-sei=Sato
en-aut-mei=Yasuo
kn-aut-name=佐藤泰雄
kn-aut-sei=佐藤
kn-aut-mei=泰雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=4-6
article-no=
start-page=307
end-page=325
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1961
dt-pub=19610630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Degenerative Changes in Human Leucocytes in Bone Marrow Tissue Culture �V. A Study on Degenerative Changes in Leucocytes of Various Leukemias and of Several Other Blood Diseases
kn-title=骨髄体外組織培養に於ける人白血球の退行性変化に関する研究 第3編 各種白血病並びに其の他二,三の血液疾患に於ける白血球の退行性変化について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the bone marrow tissue culture of various leukemias as well as of several blood diseases conducted with the purpose to see the degenerative changes of the leucocytes appearing in the growth area, the following results were obtained. 1. In the case of acute myelogenous leukemia the degenerative changes of myeloblasts proceed rapidly at an early stage of culture and there appear degeneration granules which can never be observed in normal myeloblasts. Vacuoles and abnormal processes do appear though small in number. 2. In the case of acute lymphocytic leukemia, the appearance of small degeneration granules is marked in the degenerative changes of lymphoblasts. The degenerative changes of lymphocytes proceed slightly slower than in the case of the blasts, and the nonhomogenous condensation or swelling of the nuclei can often be seen. 3. In monocytic leukemia, degenerative changes of monoblasts appear earlier than those of monocytes, and degeneration granules, small vacuoles and various abnormal processes appear most numerously as compared with other kinds of blasts. The changes of promynocytes are relatively rapid and also degeneration granules, small and intermediate vacuoles as well as various abnormal processes can be recognized. In the case of monocytes the changes occur relatively earlier than in the case of those in normal person, and appearance of numerous degeneration granules is typical, large and small vacuoles can also be observed. 4. In the case of chronic myelogenous leukemia, the degenerative changes of myeloblasts take place slower than in the case of acute myelogenous leukemia, but more rapidly than in normal person, In promyelocytes and myelocytes the changes proceed relatively slowly, and in metamyelocytes and neutrophils, the changes proceed somewhat rapidly. Generally, in the case of immature cells degeneration granules, vacuoles and abnormal processes are less in number, but in more mature cells the number of them increases. In the case of mature neutrophils spherical processes appear markedly many and eruption of nucleus is seen rather few. 5. In chronic lymphocytic leukemia degenerative changes of lymphocytes appear somewhat more rapidly than in the case of normal person but they appear slower than those in acute cases, and most of lymphocytes show homogeneous pyknosis. 6. In aplastic anemia the degenerative changes of neutrophils proceed rapidly, having large degeneration granules as well as many vacuoles. Vacolization can also be observed in a high degree. Many abnormal processes appear and the nuclei are also either swollen or erupted. 7. In essential hypochromic anemia the degenerative changes of neutrophils appear rather rapidly and degeneration grenules, small vacuoles, and spherical processes are relatively numerous. 8. The degenerative changes of neutrophils in Banti's disease progress rather rapidly and the disorder in the arrangement of specific granules can be recognized at an early stage. Degeneration granules appear rather rapidly and the nuclei are swollen relatively earlier. 9. idiopathic thrombocytopenic purpura the degenerative changes in neutrophils take place approximately in the same manner as in the case of normal person.
en-copyright=
kn-copyright=

en-aut-name=ShinagawaKoji
en-aut-sei=Shinagawa
en-aut-mei=Koji
kn-aut-name=品川晃二
kn-aut-sei=品川
kn-aut-mei=晃二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=4-6
article-no=
start-page=285
end-page=305
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1961
dt-pub=19610630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Degenerative Changes in Human Leucocytes in Bone Marrow Tissue Culture �U. Influences of Various Culture Conditions on Degenerative Changes in Neutrophils
kn-title=骨髄体外組織培養に於ける人白血球の退行性変化に関する研究 第2編 各種培養条件の好中球退行性変化に及ぼす影響について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The normal human bone marrow was cultured in vitro by the clinical tissue culture method devised in our Department in order to see the influences of such factors as the medium temperature, the hydrogen ion concentration, the osmotic pressure in the media, and some single media, on the degenerative changes of neutrophils appearing in the growth area. These results were compared with those in the case of neutrophils cultured in the normal medium. The following are the conclusions. 1. In general the degenerative changes of neutrophils proceed slowly under low temperature but more rapidly at higher temperature. At 15℃ the progress is most slow while on the contrary, at the temperature below 10℃ it is rapid, The number of degeneration granules, vacuoles, and abnormal processes decreases as the bone marrow is cultured at a lower or at a higher temperature centering around 37℃-39℃. More condensed nuclei can be observed at a high temperature, and also at a low temperature. The eruption of the nucleus appears more frequently at low temperature, however, there is almost no nuclear eruption at high temperature. 2. As for the influence of the hydrogen ion concentration, the further away is the pH from that of the normal medium the more rapid is the degenerative changes. In the acid medium the appearance of small degeneration granules is more rapid but the number of vacuoles and sbnormal processes is less, showing no tendency of fusion of nuclear lobes but a strong tendency of non-homogeneous pyknosis. In alkaline medium degeneration granules are less but vacuoles and blisters are numerous, and cytoplasm is swollen, and nucleus tends to swell up or to erupt. 3. As for the influence of the osmotic pressure, in the isotonic solution the degenerative changes appear most slowly, and in the hypotonic solution the swelling of specific granules is marked and degeneration granules are numerous and large with many vacuoles. Cytoplasm and nulcleus are swollen, especially the eruption of the nucleus is striking. In the hypertonic solution the swelling of specific granules is observed, but the number and the size of degeneration granules are small. Vacuoles and abnormal processes are less, while myelin forms can be recognized relatively many. The nucleus and cytoplasm are condensed at the initial stage of the culture but later both of them are swollen. 4. In the single medium such as physiological saline solution, Ringer's solution, serum, or in the isotonic glucose solution, the degenerative changes proceed radidly at an early stage in any of these media, and the changes progress most rapidly in the glucose solution followed by physiological saline solution and Ringer's solution. The progress is slowest in the serum medium but it is more rapid than in the normal medium.
en-copyright=
kn-copyright=

en-aut-name=ShinagawaKoji
en-aut-sei=Shinagawa
en-aut-mei=Koji
kn-aut-name=品川晃二
kn-aut-sei=品川
kn-aut-mei=晃二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=11-12
article-no=
start-page=2003
end-page=2016
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1960
dt-pub=19601231
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological Studies on Human Ascites by Means of Vital Staining and Carbon Particle Phagocytosis with Triphenyl Tetrazolium Chloride (T.P.T.) Reaction Part 2. A Study on Tumor Ascites
kn-title=生体染色並びに墨粒貪喰による人腹水の細胞学的研究 附T.P.T（Triphenyl Tetrazolium Chloride）反応 第2編 腫瘍性腹水に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vital observations were carried on with 33 examples of tumor ascites in tissue culture by means of vital staining and carbon particle phagocytosis and also cytological investigations were conducted with T.P.T reaction. With further studies on the nature and cell composition of ascites the following results were obtained. 1. The average specific gravity of ascites has been found to be 1008-1027, the average protein content 3.36 g/dl., the average cell count 584/?, and the composition of cells reveals the increase in the appearance of neutrophils, serous cells and signet ring cells among tumor cell, and these are characteristics of the ascites. By dividing these into two groups, namely one ascites group with the appearance of less than 5 per cent of tumor cells and other with the tumor cell appearance of over 20 per cent, the characteristic traits of ascites might be understood still better. 2. The confirmation of tumor cells and tumorous signet ring cells is taken as a direct indication and cytological peculiarities of other cells are considered to be an indirect indication. 3. As for the direct indication, tumor cells and tumorous signet ring cells are generally stained well with vital staining, and the stainability of the group 2 is better than the group 1. However, that of carbon particle phagocytosis is negative. 4. Cytological peculiarities of their own can be recognized in the vital observations of various cells such as those of adenoma, simple cancer, scirrhus, colloid cancer, hepatoma, coelothelioma. 5. As for the indirect indication, coarse and large granules of various cells can be recognized, and also the acceleration of neutral red staining, phagocytic ability of phagocytes and the appearance of Janus green specific granules as well as enhancement of the neutral red stainability of neutrophils and serous cells can be observed. 6. In T.P.T. reaction tumorous ascites is always positive whereas in other ascites they are negative to this test and therefore, this point proves to be a useful indirect indication. 7. For the distinction between tumorous signet ring cells and phagocytic signet ring cells, both possess their own characteristics of original cells and carbon particle phagocytosis of the former in negative, while the latter is positive.
en-copyright=
kn-copyright=

en-aut-name=KomatsubaraToshishige
en-aut-sei=Komatsubara
en-aut-mei=Toshishige
kn-aut-name=小松原利繁
kn-aut-sei=小松原
kn-aut-mei=利繁
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=7-9
article-no=
start-page=747
end-page=762
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1963
dt-pub=19630930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Leukemoid Reaction in Mouse Induced by MY Sarcom Transplantation Part �T. Studies on the tissue culture of bone marrow and spleen
kn-title=MY肉腫移植による実験的類白血病反応に関する研究 第1編 骨髄並びに脾組織培養
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hematological and histological investigations were performed in mice with myelogenous type of leukemoid reaction induced by transplantation of MY sarcom. Thereafter, tissue culture of bone marrow and spleen were carried out. The following results were obtained. 1) Tissue culture of bone marrow. The growth patterns in bone marrow culture were always normal and did not display the leukemic type throughout the entire course of leukemoid reaction. The growth ratio of the pattern increased as leukemoid reaction progressed, while the cell density was fairly constant through the course of leukemoid reaction. Wandering velocity of the neutrophils was low in the early and intermediate stages and did not show difference from the controls in the advanced stage. Cytological observations by phase contrast microscopy, vital staining and fluorescence microscopy did not revealed any difference from normal marrow cells, nor atypism which was usually seen in leukemic cells. 2) Tissue culture of spleen. The normal pattern was always observed as seen in bone marrow tissue culture through the entire course of leukemoid reaction. The growth ratio increased as the reaction progressed. However, the cell density did not show difference from the controls. In leukemoid reaction, the lymphocytic cells were gradtally replaced by the neutrophilic cells. Therefore, 70 to 80 % of the cells in the growth zone of the splenic tissue culture were occupied by neutrophilic cells. In fluorescence tissue culture, the growth zone seen in leukemoid reaction displayed diffuse reddish orange color as a result of a large number of neutrophilic cells present, while the zone observed in controls showed diffuse yellowish green color owing to abundant lymphocytes. These findings were very helpful for differentiation of the splenic tissue in leukemoid reaction from the controls. The findings described above seemed to demonstrate the development of extramedullary hematopoiesis in the spleen.
en-copyright=
kn-copyright=

en-aut-name=KaharaMasanori
en-aut-sei=Kahara
en-aut-mei=Masanori
kn-aut-name=加原雅教
kn-aut-sei=加原
kn-aut-mei=雅教
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=4
article-no=
start-page=1097
end-page=1106
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1960
dt-pub=19600330
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fluorescence-Microscopic Studies on Various Bone Marrow Cells by Tissue Culture Part 1. Fundamental Study on Fluorochrominized Bone Marrow Tissue Culture
kn-title=組織培養による骨髄諸細胞の螢光顕微鏡学的研究 第1編 螢光培養法（Fluorochrominized Bone Marrow Tissue Culture）に関する基礎的実験
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Of late many investigators have come to study blood cells with fluorescence microscope, but most of these works are carried out by supravital staining method only. Therefore, the author has attempted vital observations of bone marrow cells under the fluorescence microscope by adding fluorescent dye, acridine orange, to the medium of the simple tissue culture, the method devised in our department. For this study some fundamental studies have been conducted concerning the toxicity of dye, the selection of barrier filters, the secondary fluorescence of cells, and the influence of exciting rays on the cell growth; and obtained the following results. 1. After studying the relative growth rate, the cell density index, and the wandering velocity of neutrophils it has been found that. tissue culture is possible at low concentration, under 10(-4) of the medium with acridine orange. 2. Barrier filter, OG5 , is the most suitable one for the observation of the secondary fluorescence of the cell. 3. The concentration of the acridine orange medium at which the most distinct picture of fluorescence obtainable is at 10(-4) , and the concentration at 10(-5) is the minimum at which fluorescence can be recognized. 4. The ill effect on cells due to exciting rays may be eliminated by avoiding successive exposure to the rays. 5. The author has devised a medium for the bone marrow tissue culture, consisted of a drop of serum and a drop of physiological saline solution containing 80 γ / cc vitamin B(12) and 0.2 mg / cc acridine orange, and has designated this as the simple method of fluorochrominized bone marrow tissue culture.
en-copyright=
kn-copyright=

en-aut-name=WatanabeSusumu
en-aut-sei=Watanabe
en-aut-mei=Susumu
kn-aut-name=渡辺晋
kn-aut-sei=渡辺
kn-aut-mei=晋
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=1
article-no=
start-page=387
end-page=406
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Histamine Content of the Saliva
kn-title=唾液ヒスタミン含量に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A number of work on the saliva has been made by many authors, but chemical investigations particuarly on histamine of the saliva are few. Only those by Feldberg, Kamenowa, Sanders and Ungar et al. could be found. However, there has been no study on the histamine content of saliva at the onset of diseases in the oral cavity and its fluctuation at various kinds of surgery. Now quantiative determination was made on histamine content of the saliva in normal and pathological conditions of the oral cavity, and the relationship between the histamine content and the findings of exfoliative cytology was also investigated. One hundred and forty-five patients were examined: 46 of normal healthy control, 26 of acute inflammation, 37 of chronic inflammation, 6 of chronic non-inflammatory lesion, and 30 of minor surgery. Parotid saliva collected by Umemoto-Kakudo's Aspirator and mixed resting saliva were used as test materials. Assay of histamine was made by biological determination by means of Magnus' Apparatus after extraction of histamine by Code's Methode and then Mayeda's Method by neoantsrgan was adopted. In normal healthy controlshistamine contents of the parotid saliva and the mixed saliva were 0.015±0.002 μg/c.c. and 0.034±0.003 μg/c.c. respectively, which were almost equal to those in cases of slight degree of caries or edentulous jaws. In acute inflammation they were 0.036±0.002 μg/c.c. and 0.122±0.016 μg/c.c.. Histamine in the mixed saliva was markedly increased. In chronic inflammation they were 0.026±0.003 μg/c.c. and 0.119±0.019 μg/c.c.. Histaminee in the mixed saliva was increased especially in superficial and extensive lesions. In chronic non-inflammatory lesions they ware 0.018±0.004 μg/c.c., and 0.040±0.013 μg/c.c.. An increase of exfoliated epithelial cells, lescocytes, especially neutrophils, and bacilli were observed in acute inflammatory leasians with particulary high histamine content in the saliva. At the time of various procedures of oral surgery almost no change was observed in the parotid. saliva, while higher histamine content was noticed in the mixed saliva in major surgery than in minor one. Some considerations and discussions were made on the problems of histamine release and on the use of antihistamine preparations from the results obtained and the review of previous litrature.
en-copyright=
kn-copyright=

en-aut-name=KobayashiToshiyuki
en-aut-sei=Kobayashi
en-aut-mei=Toshiyuki
kn-aut-name=小林敏行
kn-aut-sei=小林
kn-aut-mei=敏行
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部歯科口腔外科学教室
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=10-12
article-no=
start-page=1495
end-page=1510
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19651230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies On the Effects of Cytochrome C On the Hematopoiesis of Bone Marrow Part 2 Effects of Cytochrome C On Leukopoietic Functions By Means of Bone Marrow Tissue Cuiture Supplement: Clinical Application of Cytochrome C to Some Hematological Disorder
kn-title=Cytochrome Cの骨髄造血機能に及ぼす影響に関する研究 第2編 骨髄組織培養による白血球系造血機能に及ぼす影響について 附 Cytochrome Cの2〜3の血液疾患への応用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By means of bone marrow tissue culture the author investigated the effects of cytochrome C on leukopoietic function and obtained the following results: 1) When cytochrome C was added directly to the bone marrow tissue culture of normal guinea pigs, normal persons and patients with various diseases the growthrate of outgrowth, the wandering velocity and carbon particle phagocytosis of neutrophils were increased as compared with those of the control added with saline. As the result, it has been seen that by adding an optimal amount of cytochrome C the leukopoietic functions of bone marrow were accelerated. 2) When cytochrome C was added with carcinostatic substances to the bone marrow tissue culture of normal persons, the growth rate of outgrowth, the wandering velocity and the carbon-particle phagocytosis of neutrophils were increased as compared with those of the control added with only carcinostatic substances. The similar results were obtained in the case of bone marrow tissue culture of guinea pigs of which bone marrow had been made hypoplastic by repeated administration of carcinostatic substauces and then injected with the repeated administration of cytochrome C. On the other hand, in its clinical applications, the repeated administration of cytochrome C were effective on some cases of leukopenia induced with carcinostatic substances. As it has been demonstrated that cytochrome C has some relieviug effects on the disturbance of leukopoietic functions of bone marrow successively injected with carcinostatic substances.
en-copyright=
kn-copyright=

en-aut-name=MakihataTadashi
en-aut-sei=Makihata
en-aut-mei=Tadashi
kn-aut-name=巻幡正
kn-aut-sei=巻幡
kn-aut-mei=正
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=10-12
article-no=
start-page=1355
end-page=1361
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19651230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Influences of a New Vitamin B Group on Bone Marrow Tissue Culture �T.　Influences on Bone Marrow Leukocytes
kn-title=新ビタミンB群の骨髄組織培養に及ぼす影響 第1編 骨髄内白血球系細胞に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author has investigated the influences of a so-called new vitamin B group comprising orotic acid, thioctic acid, pantothenic acid, adenine, and carnitine at the optimal medium concentrations on the outgrowth of bone marrow cells and motility of neutrophils of normal rat bone marrow cultured in vitro, and obtained the following results. 1. Stimulation of the outgrowth of bone marrow cells was seen with carnitine, adenine, pantothenic acid, thioctic acid, and orotic acid in this declining order. 2. Migrating velocity of bone marrow neutrophils was accelerated with pantothenic acid, adenine, thioctic acid, carnitine, and orotic acid in this declining order.
en-copyright=
kn-copyright=

en-aut-name=ShiraiKichiro
en-aut-sei=Shirai
en-aut-mei=Kichiro
kn-aut-name=白井吉郎
kn-aut-sei=白井
kn-aut-mei=吉郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=1-3
article-no=
start-page=295
end-page=306
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1962
dt-pub=19620330
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Influences of Salivary Gland Hormone on the Hematopoiesis of Bone Marrow Part 3. Influences of the Salivary Gland Hormone on the Bone-Marrow Tissue Culture of Patients with Hypoplastic Anemia and Banti's Disease
kn-title=唾液腺ホルモンの骨髄造血機能に及ぼす影響-骨髄体外組織培養法による- 第3編 再生不良性貧血及び所謂バンチ氏病患者骨髄培養に及ぼす影響に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to study the direct influences of the salivary gland hormone on the hematopoietic function, of bone marrow, especially on the leucocyte series of patients with hypoplastic anemia and Banti's disease by bone-marrow tissue culture (our simple method), the following results were obtained, 1. In the bone marrow tissue of the cases with hypolastic anemia type 1 (type of blood cell arrest in the bone marrow, according to the classification by Prof. Hiraki) by our simple method, the tissue growth and the wanderiug velocity of mature neutrophils were increased as compared with those of the control. On the other hand, no significant difference could be observed as compared with those of the control in type �U (type of maturation arrest) and type V (type of panmyelophtisis). 2. In the patients with Banti's disease, the leucocyte series of bone marrow was accelerated by addition of Parotin as compared with those of control, in cases with relatively normal count of nucleated myelogenous cells. On the other hand, in cases with lower count of nucleated myelogenous cells, no significant effect could be observed in the tissue culture by addition of Parotin.
en-copyright=
kn-copyright=

en-aut-name=UgakiMasaaki
en-aut-sei=Ugaki
en-aut-mei=Masaaki
kn-aut-name=宇垣公晟
kn-aut-sei=宇垣
kn-aut-mei=公晟
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=1-3
article-no=
start-page=281
end-page=287
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1962
dt-pub=19620330
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Influences of Salivary Gland Hormone on the Hematopoiesis of Bone Marrow (by means of bone-marrow tissue culture) Part 1. On the Bone-Marrow Tissue Culture of Normal Rabbits and Normal Persons
kn-title=唾液腺ホルモンの骨髄造血機能に及ばす影響―骨髄体外組織培養法による― 第1編 健康人及び家兎骨髄組織培養に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to clarify the influences of salivary gland hormone on the hematopoietic function of bone marrow, especially on the leucoyte series, Parotin was added directly in the medium of bone marrow tissue culture of normal rabbits and normal persons, and following results were obtained. 1. In the bone-marrow tissue culture of normal rabbits by hangingdrop method, the tissue growth, the wandering velocity and carbon-particle phagocytosis of pseudoeosinphils were increased as compared those of the control. 2. In the bone-marrow tissue culture of normal persons by our simple method, the tissue growth, the wandering verocity and carbon-particle phagocytosis of mature neutrophils were increased as compared with those of the control. 3. In the tissue culture added Parotin, the growth and function of leucocyte was accelerated most remarkably at the 6-hour culture.
en-copyright=
kn-copyright=

en-aut-name=UgakiMasaaki
en-aut-sei=Ugaki
en-aut-mei=Masaaki
kn-aut-name=宇垣公晟
kn-aut-sei=宇垣
kn-aut-mei=公晟
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=7
article-no=
start-page=1137
end-page=1146
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19650730
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Japanese B encephalitis virus by means of electoron microscopy and fluorescent antibody method. 3. A study on the virus antigen within cells in cerebrospinal fluid of patients with Japanese B cncephalitis
kn-title=日本脳炎ウイルスの電子顕微鏡及び螢光抗体法による研究 第3編 日本脳炎患者髄液細胞に於けるウイルス抗原の検索
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Smears of cells of cerebrospinal fluid obtained from patients with Japanese B encephalitis were examined by means of the fluorescent antibody method. Identification of cell types were maid in preparations stained with Giemsa solution after removal of fluorescein-conjugated antibodies. Specific fluorescence was observed in a part of phagocytes, which were the predominant cell type in cerebrospinal fluid in case of Japanese B encephalitis, but no fluorescence in neutrophils. A diffuse distribution of specific fluorescence was seen in the cytoplasm, and its glanular aggregation in a part of the perinuclear region of the phagocytes. Phagocytes schowing specific fluorescence were noticed in all of patients with Japanese B encephalitis and they ranged from 15% to 48% of whole cells seen in preparations. Demonstration of the viral antigen within phagocytes in cerebrospinal fluid by the fluorescent antibody method may contribute to the early diagnosis of Japanese B encephalitis.
en-copyright=
kn-copyright=

en-aut-name=TakahashiKenji
en-aut-sei=Takahashi
en-aut-mei=Kenji
kn-aut-name=高橋建次
kn-aut-sei=高橋
kn-aut-mei=建次
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=1-3
article-no=
start-page=77
end-page=90
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1962
dt-pub=19620330
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Etiology of Renal Anemia Part �V. Studies on Myelopoiesis and Thrombopoiesis by Means of Clinical Bone Marrow Tissue Culture
kn-title=腎性貧血の成因に関する研究 第3編 骨髄の臨床組織培養法による白血球系並びに栓球系造血に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The hematopoietic function of the bone marrow of renal patients and influences of azotemia on the normal bone marrow were studied by means of clinical bone marrow tissue culture. The results obtained are as follows. 1) The marrow myelopoietic function of nephrotic patients was increased, when normal human serum was added to the medium. 2) The myelopoietic function in acute glomerulonephritis showed no significant change in regard to the cell density index but the relative growth rate and cellular migratory velocity were slightly decreased, when the bone marrow was cultured in a medium containing normal human serum. 3) When the bone marrow from patients with chronic glomerulonephritis was cultured in a medium containing normal human serum, the marrow function became activated in a slightly elevated NPN group while it became dep. essed in a highly elevated NPN group. 4) The bone marrow from uremic patients cultured in a medium containing normal human serum showed a growth pattern simulating that of hypoplastic anemia. The relative growth rate was half the control value and the migratory velocity of neutrophilic cells and cell density index were markedly decreased. 5) The bone marrow from patients with chronic renal insufficiency was cultured, to investigate the megakaryocytic function, in a medium containing normal human serum. There was reduction in both megakaryocyte number and percentage of motile forms of megakaryocytes appearing in the growth zone. 6) The normal bone marrow showed a reduced relative growth rate and migratory velocity of neutrophilic cells, when cultured in a medium containing azotemic serum. 7) Similarly, the megakaryocytic function became depressed by an addition of azotemic serum to the medium. From the evifence obtained, it appears that azotemia accompanying chronic renal insufficiency exerts a depressing action on both myelopoietic and megakaryocytic functions. It is conceivable that the bone marrow under such an inhibitory action of a long standing will gradually show a transition to a hypoplastic state.
en-copyright=
kn-copyright=

en-aut-name=ArimoriShigeru
en-aut-sei=Arimori
en-aut-mei=Shigeru
kn-aut-name=有森茂
kn-aut-sei=有森
kn-aut-mei=茂
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=1-3
article-no=
start-page=13
end-page=27
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1962
dt-pub=19620330
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Nucleic Acids on Bone-Marrow Tissue Culture Part 2. Effect of nucleic acids on leucocyte and platelet series of normal and aplastic anemia human bone marrow
kn-title=核酸の骨髄体外組織培養に及ぼす影響 第2編 健康人並びに再生不良性貧血患者骨髄の白血球系及び栓球系に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=To the bone marrow of aplastic anemia and normal persons, being cultured by means of clinical tissue culture method, RNA and DNA at various concentrations were added directly to the media to see their effects on the leucoyte and platelet series. The following are the results. 1. As for the leucocyte series of normal human bone marrow, the addition of RNA as well as DNA at optimal concentration showed an increase in the growth area and cell density, while in the case of neutrophils their wandering velocity was accelerated. In the case of aplastic anemia human bone marrow the addition of both RNA and DNA at optimal concentration likewise showed a stimulating action on the leucocyte series, and this effect was further enhanced when VB12 was added concomitantly. 2. As regards the platelet series in culture, the addition of RNA and at optimal concentration acceraded the function of megakaryocytes in both bone marrow of normal persons. and aplastic anemia. 3 The stimulating effect of RNA on the platelet series was stronger than of DNA, but the effect of the two nucleic acids on the leucocyte series did not differ significantly.
en-copyright=
kn-copyright=

en-aut-name=YamasakiRyohei
en-aut-sei=Yamasaki
en-aut-mei=Ryohei
kn-aut-name=山崎良平
kn-aut-sei=山崎
kn-aut-mei=良平
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=10
article-no=
start-page=625
end-page=636
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=19641030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Leukemia in the C58 Strain of Mice Part 3. Diagnosis and Differentiation of Mouse Leukemia by the Simplified Tissue Culture Method
kn-title=C58マウス白血病の研究 第�V編 組織培養法による白血病の診断と鑑別
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The simplified tissue culture method and the fluorochrominized tissue culture method devised in our laboratory were applied to the bone marrow, spleen and lymph node of the leukemic mice of C58 strain for the purpose of diagnosis and differentiation of leukemia. And following results were obtained; 1. In the case of spontaneous leukemia, tissue culture of the lymph node and spleen showed sharply defined growth zone with increased cell density which is the characteric pattern of acute leukemia. The bone marrow showed rather diffuse border of growth zone with the appearances of many migrating neutrophiles, but cell density was remarkably increased and many leukemic cells appeared in the growth zone. 2. In the case of myelogenos leukemia, the bone marrow culture showed so-called double growth zone which is characteristic of chronic leukemia. On the other hand, the lymph node and the spleen showed diffuse border of the growth zone with increased cell density. Numerouse immature and mature neutrophilic cells appeared in the growth zone. 3. By the fluorochrominized tissue culture method, the lymph node and the spleen of lymphatic leukemia showed typical lekemic growth zone and gave diffuse yellowish-green color due to the cytoplasmic and nuclcar fluorescence of lymphoblasts and other lymphoid cells. On the other hand, the bone marrow, spleen and lymph node of myelogenous leukemia showed relatively diffuse pattern and the inner part of the growth zone gave greenish fluorescence due to the cytoplasm and nucleus of immature leukemic cell, but in the periphery of the growth zone, reddish-orange color was predominant due to the fluoresceuce of cytoplasmic granules of mature neutrophiles. In conclusion, the lymph node and spleen in lymphatic leukemia and the bone marrow in myelogenous leukemia showed typical leukemic growth zone respectively and these findings are useful for the differential diagnosis of mouse leukemia.
en-copyright=
kn-copyright=

en-aut-name=SuzukiShinya
en-aut-sei=Suzuki
en-aut-mei=Shinya
kn-aut-name=鈴木信也
kn-aut-sei=鈴木
kn-aut-mei=信也
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=10
article-no=
start-page=587
end-page=605
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=19641030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Leukemia in The C58 Strain of Mice Part 1. Hematological and histological studies of normal mice, spontaneous and transplanted leukemia mice
kn-title=C58マウス白血病の研究 第�T編 正常マウス，自然発生白血病及び移植白血病の血液学的組織学的研究並びに白血病ウイルスの電顕的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hematological and cytological studies, including peripheral blood counting, blood picture, myelogram, impressed preparation of spleen and lymph node, and phase contrast microscopic and electron microscopic observations of leukemic cells were performed on the high leukemic strain C58 mice. In the present observation, 61.4% of C58 mice developed leukemia spontaneously after the latent periods varing from 4 to 19 months. In non-leukemic mice, blood counting, peripheral-blood picture and myelogram did not show any significant change throughout the each age groups, but lymphocyt/neutrophile ratio decreased gradually after the age of 6 month. Old mice above 12 months of age showed a slight hypochromic anemia and relative neutrophilia. Most of the spontaneous leukemia occurred after the age of 6 month and 61.4% of them occurred between the age of 12 and 16 month. All of the spontaneous leukemias observed in this strain were lymphatic type. White cell count ranged from 4,500 to 225,000, averaging 109,600 with 47.2% lymphoblasts in average. Marked anemia and thrombocytopenia were present. Myelograms showed slight or moderate lymphoblastic infiltrations, but impressed preparation of spleen and lymph node always contained lymphoblasts over 90%. Morphological characteristics of the lymphoblast observed in spontaneous leukemia were narrow basophilic cytoplasm, large nucleus with a few deep indentations, coarse chromatin net work and nucleoles of vague chromatin borders. Phase contrast microscopy of lymphoblast showed nucleal indentations clearly and bright cytoplasm with a few rod-shaped mitocondrias in the Golgi area. Electron microscopic observations of lymphoblast also revealed the characteristic indentations of the nucleus. Spontaneous leukemia could be transplanted easely to the adult C58 mice by cell graft. A transplanted line named OHS-LL, No 1 is being maintained serially up to present. Serially transplanted leukemia mice often showed remarkable myeloid reaction and died after 7-8 days after cell graft. Blastic cells became to show atypical form suggesting of much more primitive and malignant character than that of the original leukemia by serial transplantations. Electron microscopic observations of lymph node and spleen of spontaneous leukemia revealed the presence of numerous virus particles located extracellularly. These particles were morphlolgically identical with the C particle observed in various other leukemic mice.
en-copyright=
kn-copyright=

en-aut-name=SuzukiShinya
en-aut-sei=Suzuki
en-aut-mei=Shinya
kn-aut-name=鈴木信也
kn-aut-sei=鈴木
kn-aut-mei=信也
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=5-6
article-no=
start-page=865
end-page=876
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19650630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Hematopoetic Functions in the Bone Marrow of the Patients with Silicosis Part II. Studies on the Leucopoetic Functions in the Bone Marrow
kn-title=珪肺患者の骨髄造血機能に関する研究 第2編 白血球系骨髄造血機能に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to pursuit the leucopoetic functions in the bone marrow of the patients with silicosis, grawth ability of the cultured bone marrow and functions of the cells in the bone marrow were observed on the clinical tissue culture method of the bone marrow in the silicosis patients. Following results were obtained. 1) The growth ability of the cultured bone marrow were demonstrated within normal limits in the grawth area and cell density in the patients with chest x-ray findings of I-II and III type, while somewhat depressed in IV type. Definite depression was obrerved in C of IV type. This tendency was mostly remarkable in the findings of the cell density. 2) Functions of the bone marrow cells such as wandering velosity and phagocytic function of the india ink of the neutrophilic leucocytes were normal in I-II anb III type of the chest x-ray, and almost normal in A and B of IV type, while inhibition of the function was observed in C of IV type,
en-copyright=
kn-copyright=

en-aut-name=NishimuraFumio
en-aut-sei=Nishimura
en-aut-mei=Fumio
kn-aut-name=西村文夫
kn-aut-sei=西村
kn-aut-mei=文夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=4-6
article-no=
start-page=259
end-page=266
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=19640630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological studies on the exudates formed with the application of Usguentum Vesicans Part �V. Studies on the exudate in patients with chronic leukemia
kn-title=発泡膏貼布による滲出液の細胞学的研究 第3編 慢性白血病患者の滲出液について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cellular reactions were studied through an investigation of cellular aspects of exudate formed with the application of Unguentum Vesicans to patients with chronic ieukemia.　Following are the results. 1. As compared with normal controls, some delay was noted in the onset of blister formation and numbers of cells were less, but the decrease was not so remarkable as in acute leukemia. 2. Usually, the cells consist mostly of polymorphonuclear neutrophils, and in addition a few lymphocytes, monocytes, staff neutrophils, eosinophils, and basophils were noted. In chronic myelogenous leukemia, basophils were noted in higher percentage. 3. The average numbers of nucleus segmentation of the neutrophils were about the same as in normal controls. 4. In chronic myelogenous leukemia, wandering velocity of the neutrophils was decreased as compared with normal controls, but the decrease was not so remarkable as in acute leukemia. In chronic lymphocytic leukemia, the velocity was about the same as in normal controls. 5. In chronic myelogenous leukemia, cell numbers and wandering velocity of the neutrophils were remarkably increased during remissions as compared with the pre-treatment period.
en-copyright=
kn-copyright=

en-aut-name=SakikawaYoshinobu
en-aut-sei=Sakikawa
en-aut-mei=Yoshinobu
kn-aut-name=咲川嘉信
kn-aut-sei=咲川
kn-aut-mei=嘉信
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=4-6
article-no=
start-page=249
end-page=258
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=19640630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological studies on the exudates formed with the application of Unguentum Vesicans Part �U. Studies on the exudate in patients with acute leukemia and monocytic leukemia
kn-title=発泡膏貼布による滲出液の細胞学的研究 第2編 急性白血病並びに単球性白血病患者の滲出液について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cellular reactions were studied through an investigation of cellular aspects of exudate formed with the application of Unguentum Vesicans to patients with acute leukemia and monocytic leukemia. Followings are the results. 1. As compared with normal controls, some delay was noted in the onset of blister formation and numbers of cells were less. The decrease was especially remarkable up to 6 hours following the onset of blister formation and in some cases, acellular exudate was noted. 2. The cells consist mostly of polymorphonuclear neutrophils, and in addition a few lymphocytes, monocytes, and staff neutrophils were noted. Apperance of immature cells was noted in exceptional cases. 3. The average numbers of nucleus segmentation of the neutrophils were less as compared with normal controls. 4. Wandering velocity of the neutrophils was remarkably decreased as compared with normal controls. 5. In acute lymphocytic leukemia and monocytic leukemia, numbers of cells were increased during remissions.
en-copyright=
kn-copyright=

en-aut-name=SakikawaYoshinobu
en-aut-sei=Sakikawa
en-aut-mei=Yoshinobu
kn-aut-name=咲川嘉信
kn-aut-sei=咲川
kn-aut-mei=嘉信
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=4-6
article-no=
start-page=239
end-page=247
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=19640630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological studies on the exudates formed with the application of Unguentum Vesicans. Part �T. In normal coutrols
kn-title=発泡膏貼布による滲出液の細胞学的研究 第1編 正常人について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to study the defense reaction of the body to a local infection, an investigation was made for cellular aspects of exudate formed with the application of Unguentum Vesicans. Followings are the results. 1. The onset of blister formation averaged 8,7 hours following the application of Unguentum Vesicans. 2. Apperance of cells was noted with an increase of the exudate following blister formation. Numbers of cells in the exudate in 12 cases 1,3,6 and 12 hours following the onset of blister formation averaged 1,037, 1,481, 1,245, and 2,083 respectively. 3. The cells conist mostly of polymorphonuclear neutrophils, and in addition a few lymphocytes, monocytes, eosinophils, and staff neutrophils were noted. 4. The average numbers of nucleus segmentation of the neutrophils in 12 cases 1,3,6, and 12 hours following the onset of blister formation were 2.85, 3-08, 3.14, and 3.32 respectively, showing a constant increase with passage of time. 5. Wandering velocity of the neutrophils in 12 cases 1,3,6, and 12 hours following the onset of blister formation averaged 10.04μ/min, 12.57μ/min, 10.76μ/min, and 11.06μ/min respectively, showing a relatively active movement constantly. 6. An investigation of the exudate formed with the application of Unguentum Vesicans has a merit in that calculation of cell numbers and measurement of the wandering velocity can be performed with ease.
en-copyright=
kn-copyright=

en-aut-name=SakikawaYoshinobu
en-aut-sei=Sakikawa
en-aut-mei=Yoshinobu
kn-aut-name=咲川嘉信
kn-aut-sei=咲川
kn-aut-mei=嘉信
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=1-3
article-no=
start-page=105
end-page=112
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=19640330
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Influences of ATP on Hematopoietic Functions of Bone Marrow 1. Influences of ATP on Leukopoietic Functions of Rabbit, Normal Human and Hypoplastic Marrow
kn-title=Adenosine triphosphate (ATP)の骨髄造血機能に及ぼす影響に関する研究 第1編 骨髄組織培養に於ける白血球系造血機能に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to observe the influences of ATP on the bone marrow leukopoiesis, clinical tissue culture of bone marrow from normal rabbits, normal persons and patients with hypoplastic anemia was conducted. ATP solutions at various concentrations were added directly to the culture media and observations were carried out to see effect of ATP on the functions of neutrophils of bone marrow. As a result, it has been demonstrated that by adding an optimal amount of ATP the ontgrowth, cell density and wandering velosity of neutrophils were increased.
en-copyright=
kn-copyright=

en-aut-name=OkamotoMichio
en-aut-sei=Okamoto
en-aut-mei=Michio
kn-aut-name=岡本迪男
kn-aut-sei=岡本
kn-aut-mei=迪男
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医学研究科
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=1
article-no=
start-page=59
end-page=69
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19650130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Studies on X-ray and Leukemia Part 1. A. Comparative Study of the Effect of X-Irradiation on the Peripheral Blood Picture in Various Strains of Mice
kn-title=放射線と白血病に関する実験的研究 第1編 X線照射の末梢血液像に及ぼす影響に関する各種系統マウスの比較研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=As one of the series of studies on radiation leukemogenesis, changes in the peripheral blood picture occurring with lapse of time were pursued with the Cb, D103, StA, and Zb strains of mice (30-50 days old) after a single whole body X-irradiation of 350 r. The results are summarized as follows. 1. Both erythrocyte counts and hemoglobin contents showed the minimum values 2 weeks after the irradiation, and after recovering once they tended to decrease again. Reticulocytes decreased to about 2-4‰ immediately after the irradiation but by the second week they increased to about twice the number in normal condition, and thereafter they returned to the normal count. This change was most marked in the D103 strain, but ultimately the changes in the erythrocyte series presented similar curves in all the four strains mentioned above, and these changes did not differ from those observed in RF strain. 2. The number of leukocytes decressed rapidly to as low as 2,000 immediately after the X-irradiation. The susceptibility to X-ray irradiation was higher in the leukocyte series than in the erythrocyte series. 3. Lymphopoiesis was more sensitive to X-irradiation than myelogenous hematopoiesis, and the decrease of lymphocytes was greater than that of neutrophils, showing a relative neutrophilia for several weeks after the irradiation. 4. After once recovering, the leukocyte counts in the Cb, D103, StA, and Zb strains of mice all did not show such a transient decrease as observed in the preleukemic stage of RF strain about 13 weeks after the irradiation. In addition, lymphocytic leukemia did not develop. 5, In the Cb, D103, StA, and Zb strains irradiated with X-ray there could be recognized no severe eosinophilia such as observed in the RF strain that received X-irradiation. From these findings it has been confirmed that there can be recognized no marked differences in the peripheral blood picture immediately after a single whole body X-irradiation of 350 r between the RF strain of mice that develop leukemia by X-ray, and the Cb, D103, StA. and Zb strains that do not develop leukemia by X-irradiation, but in the observations conducted thereafter along with lapse of time there can be seen several distinct differences in the changes of the leukocyte series between them.
en-copyright=
kn-copyright=

en-aut-name=IkejiriKoji
en-aut-sei=Ikejiri
en-aut-mei=Koji
kn-aut-name=池尻孝治
kn-aut-sei=池尻
kn-aut-mei=孝治
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=1
article-no=
start-page=25
end-page=48
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19650130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Leukemogenesis of Mouse Leukemia by X-irradiation. Part 2. Classification of Leukemia and Various Factors Involved in Leukemogenesis.
kn-title=X線照射によるマウス白血病の発生機構に関する研究 第2編 白血病の種類と白血病発生に及ぼす諸因子について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=For the elucidation of leukemogenesis of mouse leukemia induced by X-ray, a study was carried out on various factors influencing the development of leukemia. Namely, an effort was made to see the relationship between such factors as the age of mice receiving X-irradiation, the dose and the method of irradiation, the strain and sex of mice on one hand and the rate of leukemia induction on the other. Also a comparative study was made of the types of mouse leukemia elicited by X-ray. The results of the study are stated as follows. 1. In mice of the RF strain given one whole body X-irradiation of 350 r, and in both C(57)BL and C(3)Hf mice given four who1e body irradiations of 160 r each at the interval of 4 days to the total dosage of 600 r, the rate of leukemia induction was 77.3%, 88%, and 20% in the respective groups. 2. It has been demonstrated that the rate of leukemia induction was highest in mice of the RF strain 30-40 days old, and this rate decreased along with advancement in the age (several months old) of the mice. 3. As for the rate of leukemia induction in mice of the RF strain 30-40 days old, that received one whole body X-irradiation of 350 r, 22 (78.5%) out of 28 male mice developed leukemia while 19 (73.0%) out of 26 female mice, developed leukemia, showing no significant difference in sex. 4. It has been found that the rate of leukemia induction in the RF strain mice of 30-40 days old after one whole body X-irradiation increases with the dose of the irradiation, reaching its maximum at the dose of 350 r, but with the dose of 450 r the rate of leukemia induction rather decreases. 5. Of lymphocytic leukemia induced in the RF strain mice by X-irradiation, there were two types, that is, thymoma type and non-thymoma type, and there could be observed distinct differences in the peripheral blood pictures as well as in the patterns of cell infiltration into the liver between these two types. In addition, some of these lymphocytic leukemic mice presented a neutrophilic leukemoid reaction. There was only one mouse that developed myelogenous leukemia.
en-copyright=
kn-copyright=

en-aut-name=SotaSusumu
en-aut-sei=Sota
en-aut-mei=Susumu
kn-aut-name=宗田範
kn-aut-sei=宗田
kn-aut-mei=範
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=79
cd-vols=
no-issue=11-12
article-no=
start-page=957
end-page=968
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1967
dt-pub=19671230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Morphological Studies of Effects of Chloroquine　Diphosphate on Fibroblasts and Tumor Cells　�T. Phase Contrast Microscopic and Cytochemical Study
kn-title=燐酸クロロキンの線維芽細胞および腫瘍細胞に及ぼす影響の形態学的研究 第1編 位相差顕微鏡並びに細胞化学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effects of chloroquine diphosphate on fibroblasts, Yoshida sarcoma cells, Ehrlich ascites tumor cells and human neutrophils were investigated morphologically by phase contrast microscopy and cytochemical methods. The following results were obtained. 1) Fibroblasts: The fibroblasts obtained from mice previously administered with 25 mg of chloroquine diphosphate per kilogram body weight for five days showed increase of vacuoles and fat droplets in the cytoplasm. The cultured fibroblasts displayed increase of intracytoplasmic fatdroplets by addition of such small amount of chloroquine diphosphate as 2γ per 100 ml to the culture medium. As the concentration of chloroquine became high, increase in size and number of the intracytoplasmic lipid droplets and vacuoles and fragmentation of the mitochondria were observed. 2) The main morphological changes of Yoshida sarcoma cells and Ehrlich ascites tumor cells were increased of intracytoplasmic vacuoles and lipid droplets both in in vivo and in vitro chloroquine treatment. However, these changes of tumor cells were considerably mild when compated with those of fibroblasts which underwent the same treatment. 3) By the treatment of chloroquine, Yoshida sarcoma cells and Ehrlich ascites tumor cells disclosed a slight incrcase of the granules positive in PAS staining. 4) When treated with chloroquine, fat granules of Yoshida sarcoma cells and Ehrlich ascites tumor cells were observed to be increased in size and number in lipid staining. 5) Numerous granules were observed in neutrophils of patients who were administered with chloroquine disphosphate for a long period. However, patien's tumor cells showed no morphological changes. 6) In summary, chloroquine diphosphate can exert mosphological alteration on various cells which was considered to be non-specific cellular degeneration.
en-copyright=
kn-copyright=

en-aut-name=HiraokaToshinobu
en-aut-sei=Hiraoka
en-aut-mei=Toshinobu
kn-aut-name=平岡敏延
kn-aut-sei=平岡
kn-aut-mei=敏延
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=79
cd-vols=
no-issue=9-10
article-no=
start-page=743
end-page=750
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1967
dt-pub=19671030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Intracellular Crystals �T. New Crystals Arising in Bone Marrow Cells of Various Animals
kn-title=細胞内に生ずる結晶に関する研究 第1編 各種動物骨髄細胞内に生ずる新結晶
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hitherto unknown intracellular crystallizations were discovered in neutrophils and megakaryocytes of various animals. Animals used were normal C(57) and Cb mice, normal hybrid mice. these mice with leukemoid reaction induced by Saponin injection, C(58) mice with myelogenous leukemia (OHS ML, No. 1), C(58) mice with lymphocytic leukemia (OHS-LL, No. 1), normal rats, guinea-pigs and dogs. Cells of bone marrow or spleen of these animals were observed by means of tissue culture or supravital preparations. A few hours after the preparations were m de,   shape , nee e shaped, or: pindle-shaped, strongly refractile crystals were observed arising in neutrophils and later in megakaryocytes. Those crystals were positive in birefringence and do not seems to be of extracellular origin. The crystals I found have not been reported in the literature.
en-copyright=
kn-copyright=

en-aut-name=MatsumoriHiroshi
en-aut-sei=Matsumori
en-aut-mei=Hiroshi
kn-aut-name=松森宏
kn-aut-sei=松森
kn-aut-mei=宏
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=82
cd-vols=
no-issue=9-10
article-no=
start-page=373
end-page=378
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1970
dt-pub=19701030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Influence of Quinolin Derivatives on Hematopoietic Functions of Bone Marrow 1. Influences of Chlorcquine on Leukopoietic Functions by Clinical Tissue Culture
kn-title=キノリン誘導体の骨髄造血機能に及ぼす影響に関する研究 第1編 骨髄臨床組織培養法による白血球系造血機能に及ぼすクロロキンの影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to observe the influences of chloroquine on the bone marrow leukopoiesis, clinical tissue culture of bone marrow from normal rabbits was conducted. Chloroquine diphosphate solutions at various concentrations were added directly to the culture media and observations were carried out on the outgrowth, cell density, wandering velosity of neutrophils, carbon-particle phagocytosis and vital staining. As a result, it has been demonstrated that the leukopoietic functions of bone marrow were hardly inhibited in the medium containning 2γ/dl and 20γ/dl of chloroquine, but were markedly inhibited in the medium containning 200γ/dl and 2000γ/dl of chloroquine.
en-copyright=
kn-copyright=

en-aut-name=HayashiNobuhiro
en-aut-sei=Hayashi
en-aut-mei=Nobuhiro
kn-aut-name=林信広
kn-aut-sei=林
kn-aut-mei=信広
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=79
cd-vols=
no-issue=3-4
article-no=
start-page=153
end-page=166
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1967
dt-pub=19670430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Tissue Culture of Spleen Part 3. On the Clinical Application
kn-title=脾臓組織培養に関する研究 第3編 臨床応用について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author employed securely laparoscopy in performing direct vision splenic biopsy, cultured the biopsied human splenic tissue using the clinical tissue culture method of spleen, and obtained the following conclusions. 1) In the lighting observation of spleen using laparoscopy , about half the number of both leukemia and Banti's disease showed change in the character of surface , namely, color,
hardness, enlargement, atrophie, unevenness and adhesion. In the histological observations of biopsied splenic tissue, although all cases of lekemia showed the infiltration of leukemic cells, the determination of infiltrated cells in an acute lymphocytic leukemia and two cases of monocytic leukemia was difficult. In adout 70 per cents of Banti's disease the histological observations which suspected this disease were shown. In the splenogram of all cases of leukemia, the infiltration of various leukemic cells was seen respectively, and the determination of infiltrated cells was possible. While in the splenogram of Banti's disease no significant pathological findings were obtained. 2) In the splenic tissue culture of acute leukemia including monocytic leukemia, the boundaries of the growrh zone were well-defined and clear-cut, the cell density increased markedly and cells in the growth zone were mostly lymphoblasts and young lymphocytes in the case of acute lymphocytic leukemia, myeloblasts and immature granulocytic cells in the case of myelogenous leukemia, and monoblasts and promonocytes in the case of monocytic leukemia. 3) In chronic myelogenous leukemia double growth zone was not shown but the similar pattern to that of acute leukemia was seen. 4) In leukemoid reaction the pattern of splenic tissue growth was similar to that of normal human spleen, however in the growth zone numerous myelogenous cells, many of which were immature, were seen. 5) In Banti's disease the growth was somewhat reduced. In the terminal stage, splenic reticulum cells and fibroblasts tended to increase. 6) In aplastic anemia the growth pattern was similar to that of normal spleen, howevrer a few immature myelogenous cells were always found in the rowth zone. 7) In chronic Werlhof's disease the growth pattern gshowed that of normal spleen, except that many megakaryocytes with diminished function appeared in the growth zone. 8) In leukemic reticuloendotheliosis the growth pattern resembled that of acute lekemia. The growth zone was composed of the same lymphoblastoid cells as found in the peripheral blood and larger young cells. 9) In infection the growth pattern resembled the normal but its growth was remarkable and mature neutrophils were increased markedly. From these results the author thought that the clinical tissue culture method of spleen, when it was performed in company with the clinical tissue culture method of bone marrow, had important clinical significance for making clear various blood diseases and other diseases mit splenomegaly.
en-copyright=
kn-copyright=

en-aut-name=ShimazakiKoichi
en-aut-sei=Shimazaki
en-aut-mei=Koichi
kn-aut-name=島崎孝一
kn-aut-sei=島崎
kn-aut-mei=孝一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=81
cd-vols=
no-issue=7-8
article-no=
start-page=487
end-page=494
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1969
dt-pub=19690830
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cytological Studies on Human Pleural Effusion by Tissue Culture Part 2. A study on pathologic pleural effusion
kn-title=体外組織培養法による人胸水の細胞学的研究 第2編 人病的胸水に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the vital observations of 8 cases of cancer, 10 cases of tuberculosis and 2 cases of nephrosis by means of tissue cultures of pleural effusion of respective group, the author obtained the following results: (1) In the case of cancer, the average cell count is 720/m?. The characteristics of the cell composition are the appearance of tumor cells and tumorous signet-ring cells, a slight increase in the number of serous cells, a moderate increase of neutrophils and the appearance of phagocytic-ring cells. (2) In the case of tuberculous pleurisy, the average cell count is 1564/m?. There can be recognized such characteristics as a increase of small size phagocytics and lymphocytes. The movement of these cells are slightly disturbed compare with non-pathologic condition. (3) In the case of nephrosis, the average cell count is 180/m?. The characteristic of the cells is increase of small phagocyte and serous cells, but most of cells are degenerated in the early stage.
en-copyright=
kn-copyright=

en-aut-name=SonobeTakeshi
en-aut-sei=Sonobe
en-aut-mei=Takeshi
kn-aut-name=園部武
kn-aut-sei=園部
kn-aut-mei=武
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=86
cd-vols=
no-issue=3-4
article-no=
start-page=139
end-page=145
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1974
dt-pub=19740430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the exudates formed by the application of Uneuentum vesicans. Part �T. Cytological studies
kn-title=発泡膏貼布による滲出液の研究 第1編 細胞学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to study the difference of defense reactions to local stimulation in various diseases, an experiment was performed to examine cellular characteristics of exudates produced by the local application of Unguentum vesicans.
The results of the study are summarized as follows. (1) The average time to the onset of blister formation following the application of Unguentum vesicans was 13.3 hours, and had a tendency to be delayed by aging process, particularly in an old age group with lung tuberculosis. (2) The average volume of the exudates at 24 hours after the application was 0.09cc/c�u, and had a tendency to be decreased by aging process and in patients with consumptive diseases. (3) The average number of cells in the exudates at 24 hours after the application was 2410/m?, and had no relationship to the age or disease processes of patients. (4) The majority of the cells in the exudates consisted of polymorphonuclear neutrophils with a few non-segmented neutrophils, lymphocytes, monocytes and eosinophils. The cellular composition in the exudates was little altered by the age or disease processes of patients. (5) The investigation of the exudates produced by the application of Unguentum vesicans may be useful in the evaluation of local inflammatory responses in various disease states.
en-copyright=
kn-copyright=

en-aut-name=KuroseKenkichi
en-aut-sei=Kurose
en-aut-mei=Kenkichi
kn-aut-name=黒瀬健吉
kn-aut-sei=黒瀬
kn-aut-mei=健吉
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科
END

start-ver=1.4
cd-journal=joma
no-vol=83
cd-vols=
no-issue=9-10
article-no=
start-page=343
end-page=356
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1971
dt-pub=19711030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=CLINICAL STUDIES ON LEUKEMOID REACTION �V. Leukemoid reaction of the blood cells other than leukemic series in leukemia
kn-title=類白血病反応に関する臨床的研究 第3編 白血病における他種血球系統の類白血病反応
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=As a link in the series of studies on the leukemoid reaction, mainly the bone marrow tissue cultures were carried out together with the studies of clinical symptoms on the 162 leukemia patients admitted to our Clinic for the period of from January, 1954 to December, 1960. The following conclusions were drawn. Fifty-five cases (34.0%) out of the 162 patients of leukemia revealed leukemoid reaction. The incidence of the leukemoid reaction proved to be high in the patients with acute lymphocytic leukemia and chronic myelogenous leukemia, followed by monocytic leukemia, and some cases in acute myelogenous leukemia. The severity of the leukemoid reaction was relatively mild in the majority of cases, but some show a marked reaction making the type difficult to discern. In such instances, it was possible to identify a composing cell series by the tissue culture method. As for the cell constituents in the blood attributed to leukemoid reaction in acute myelogenous leukemia, it was either of the neutrophil, the eosinophil or the monocyte series. In Xacute lymphocytic and monocytic leukemias it was mostly of the neutrophil series and, in some cases, either of the eosinophil or the basophil series. In chronic myelogenous leukemia it consisted of both the eosinophil and basophil series. As can be seen from these findings, in leukemias, the leukemoid reaction often associated with other white cell series than their own leukemia cell types. Therefore, the diagnosis of a type of leukemoid reaction should be made carefully.
en-copyright=
kn-copyright=

en-aut-name=SaibaraTatsuo
en-aut-sei=Saibara
en-aut-mei=Tatsuo
kn-aut-name=西原達郎
kn-aut-sei=西原
kn-aut-mei=達郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END