result 11275 件
| JaLCDOI | 10.18926/11820 |
|---|---|
| Title Alternative | Photon background caused by the reduction of the electron beam energy - Materials of scattering foil - |
| FullText URL | 003_013_018.pdf |
| Author | Nakagiri, Yoshitada| Mimura, Seiiti| Inamura, Keiji| Tahara, Seiji| Miyake, Hideaki| Azuma, Yoshiharu| Egusa, Tomomi| Mikami, Yasutaka| Hiraki, Yoshio| Yamada, Toshiharu| Sugita, Katsuhiko| |
| Abstract | The total skin electron beam therapy has been one of the clinical treatment for peripherally T-cell lymphoma; Mycosis fungoides, adult T-cell lymphoma, and so on. The crucial points in this treatment are not only having an optimum energy level of electron beam for a target volume (a tissue) but also keeping the photon back ground low. It is not easy to regulate those points by the control panel, however, for the equipment that is conventinally used for electron beam, theoretically, is to exchange lead (Pb), which is ordinarily used, to a low atomic number material as a scattering foil. We examined several different kinds and / or various thickness as a scattering foil material that can make the electron beam lower without an increase of the contaminant as X-ray. We hereby reported the results, and strongly suggested the following two materials in use; acrylic plate, carbon board, and so on, which are easily available and worked, would be practically useful for the total skin electron beam therapy. |
| Keywords | 電子線エネルギー X線混入率 電子線全身照射法 スキャタリングホイル |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1993-01-31 |
| Volume | volume3 |
| Start Page | 13 |
| End Page | 18 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313375 |
| Author | 岡山大学医療技術短期大学部| |
|---|---|
| Published Date | 1994-01-31 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Volume | volume4 |
| Content Type | Others |
| Author | 岡山大学医療技術短期大学部| |
|---|---|
| Published Date | 1995-01-31 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Volume | volume5 |
| Content Type | Others |
| Author | 岡山大学医療技術短期大学部| |
|---|---|
| Published Date | 1997-01-31 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Volume | volume7 |
| Issue | issue2 |
| Content Type | Others |
| Author | 岡山大学医療技術短期大学部| |
|---|---|
| Published Date | 1993-01-31 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Volume | volume3 |
| Content Type | Others |
| Author | Katsuno, Gotaro| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | 岡山大学医療技術短期大学部| |
|---|---|
| Published Date | 1996-02-29 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Volume | volume6 |
| Content Type | Others |
| JaLCDOI | 10.18926/11789 |
|---|---|
| Title Alternative | Enzyme Immunoassay for Rabbit C-reactive Protein |
| FullText URL | 002_023_028.pdf |
| Author | Mori, Shuji| |
| Abstract | Sensitive enzyme Immunoassay method specific for rabbit C-reactive protein was established. This was based upon specific Ca(2+)-dependent binding profile of C-reactive protein for some compounds containing phosphorylcholine moiety intramolecularly. By this method, more than 0.001μg of rabbit C-reactive protein was detectable. Specific binding of C-reactive protein for p-aminophenylphosphorylcholine immobilized on solid phase was inhibited by either addition of EDTA or phophorylcholine analogues, effectively. It may be useful to study possible roles of C-reactive protein in inflammatory regions. |
| Keywords | C-reactive Protein Enzyme Immunoassay |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1992-02-05 |
| Volume | volume2 |
| Start Page | 23 |
| End Page | 28 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313528 |
| Author | 岡山大学医療技術短期大学部| |
|---|---|
| Published Date | 1992-02-05 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Volume | volume2 |
| Content Type | Others |
| Author | Matsumura, Takuhiro| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11778 |
|---|---|
| Title Alternative | Determination of Hematinic Acid Produced by Oxidative Cleavage of Hemoglobin Heme in Red Blood Cells |
| FullText URL | 001_077_082.pdf |
| Author | Hirota, Kazuhiro| Sasaki, Kenji| Hirota, Takashi| |
| Abstract | Our previous studies on the mechanism of phenylhydrazine-induced hemolytic anemia have shown that hematinic acid, one of oxidative cleavage products of heme, is formed by the reaction of hemoglobin with phenylhydrazine. Develoment of the determination of hematinic acid formed by this reaction in red blood cells (RBC) was required to study the mechanism of the hemolysis. Hemolysates prepared by lysis of fresh human RBC with water was mixed with standard hematinic acid. A solution consisting of hydrochloric acid, methanol, and acetone was added, and most of hemoglobin precipitated was removed by centrifugation. Hematinic acid was derived to the methyl ester by incubation with methanol containing sulfuric acid. The ester was passed to two type of silica gel column to remove interferences, and was analysed on a reversedphase high-performance liquid chromatographic column. Hematinic acid could be determined in the range 1.0-20.0μmol/ml RBC. Recovery from hemolysate was 65.0% ±3.5%. Standard compounds of hematinic acid and its methyl ester were prepared by the oxidation of hemin with hydrogen peroxide, and were comfirmed by elemental analyses and mass spectra. |
| Keywords | hematinic acid high-performance liquid chromatography red blood cells heme |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 77 |
| End Page | 82 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313412 |
| Author | Zhao, Ying| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Uchida, Akiko| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11763 |
|---|---|
| Title Alternative | Immunological Abnormalities of Alveolar Macrophages in Patients with Sarcoidosis |
| FullText URL | 001_039_050.pdf |
| Author | Nakata, Yasunari| |
| Abstract | Bronchoalveolar lavage is an invaluable means of accurately evaluating the inflammatory and immune processes in the human lung. Sarcoidosis is a multisystem disorder characterized by heightened immune processes at sites of disease activity. The lung is most commonly involved. Although granulomas are charactaristic pathologic features of this disease, a number of studies suggest that the initial lesion in the lung is a T-cell alveolitis (an accumulation of T-cellls in the lung). There are a lot of findings that show abnormal functions of alveolar macrophages obtained by bronchoalveolar lavage in the release of various monokines and arachidonic acid metabolites and metabolize oxygen. In this review, the abnormalities of alveolar macrophages implicated in pulmonary T-cell alveolitis and fibrosis are reviewed and their potential roles in the lungs are discussed. |
| Keywords | sarcoidosis alveolar macrophage monokine metabolize oxygen arachidonic acid metabolite |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 39 |
| End Page | 50 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313772 |
| Author | Harada, Daisuke| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11759 |
|---|---|
| Title Alternative | 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について― |
| FullText URL | 007_1_009_015.pdf |
| Author | Sakiyama, Junko| Ichimura, Mitsuko| Tohge, Hiroko| Endo, Hiroshi| Kawakami, Kaori| Kawatani, Yuki| |
| Abstract | Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues. |
| Keywords | PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 15 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313519 |
| JaLCDOI | 10.18926/11757 |
|---|---|
| Title Alternative | Lipid Peroxides (TBA reactive substances)and Fatty Acid Compositions in Mouse Serum with Whole-body Irradiation and in Tumor-bearing Mouse Serum with Local Irradiation |
| FullText URL | 001_029_037.pdf |
| Author | Yamamoto, Goki| |
| Abstract | Effects of irradiation on lipid peroxides of mouse serum, Ehrlich solid tumor-bearing mouse serum, which tomor cells were transplanted to the leg, and its tumor tissue were studied by a thiobarbituric acid (TBA) color reaction in the acetic acid condition with (Fe-TBA value) or without (TBA value) ferrous ammonium sulfate. TBA reactive substances were caluculated into the amount of malondialdehyde. Besides, fatty acid and lipid compositions were analyzed as a substrates. Irradiated samples were isolated at 3 days after irradiations. The TBA value of normal mouse serum was expressed 17nmoles/ml of serum, and Fe-TBA value gave 2.4-fold of the TBA value. Although an increase TBA value was not observed by whole-body irradiation, a significant increase of Fe-TBA value was measured, indicating 2.5-fold at 10Gy exposure compared with the normal Fe-TBA value, and 5.5-fold with the TBA value. The TBA value of tumor-bearing mouse serum was 14nmoles/ml as a low rate to that of normal serum, but the Fe-TBA value gave same magnification of that of normal one. The TBA value of tumor-bearing mouse serum was not changed by the local irradiation to the tumor region of leg, but the Fe-TBA value was increased 2.8 and 4.4 times at 10Gy and 20Gy exposures, respectively, comparing with the non-irradiated one, and 7.7 and 10.5 times with the TBA value, respectively. The TBA value of solid tumor homogenate exhibited 1.16nmoles/mg of protein, and the Fe-TBA value, pointed out 5 times as much as the TBA value. Both values slightly increased by the irradiation. These facts suggest that a shift of TBA value of serum hard to get, but Fe-TBA value of serum distinctly increases by the irradiation. The fatty acid composition of mouse serum lipids showed an increment of relative percentages of linoleic and arachidonic acids by the whole-body irradiation. The relative percentage of fatty acid compositon of lipids from solid tumor-bearing mouse serum was similar tendency to that of normal one, and the local exposure to the tumor part was not affected the each percentage. The percentage of high unsaturated fatty acid of tumor lipids similar to that of serum lipids, and a decrease of the percentage of arachidonic acid was accounted by the irradiation. In relative percentages of lipid compositions, the percentage of choleste rolester of serum lipids increased by the whole-body irradiation, and that of phospholipid fraction was not changed. In the case of tumor-bearing mouse serum lipids, an increment of the percentage of cholesterolester was obtained as compared with that of normal serum lipids, and this percentage decreased by the local irradiation to tumor part with an increment of the percentage of phospholipid. The percentage of phospholipid in tumor lipids had decreased by the irradiation. The facts suggest that a cause of the increased Fe-TBA value of tumor-bearing mouse serum by the irradiation to tumor is due to the release of peroxidizable phospholipid into serum from the damaged menbranes of tumor cells at large doses to exposure. |
| Keywords | Lipid peroxides Irradiation Mouse serum Tumor-bearing mouse serum Fatty acid composition |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 29 |
| End Page | 37 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313903 |
| JaLCDOI | 10.18926/11756 |
|---|---|
| Title Alternative | 大腸菌を用いたフォスファカン（コンドロイチン硫酸プロテオグリカン）の融合コア蛋白の発現条件の検討 |
| FullText URL | 006_063_072.pdf |
| Author | Ito, Sekiko| Okamoto, Motoi| |
| Abstract | Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA fragments inserted into the expression vector (pGEX-4T-1) were amplified by RT-PCR using mRNA purified from E18 rat brain as template. Primers attached with BamH I or EcoR I restriction site on 5' end were used to amplify first strand cDNA by PCR. Before ligation into the pGEX-4T-1 for GST fusion protein, PCR products were once cloned using T-A cloning system because they were not directly ligated into the pGEX-4T-1. E.coli strain BL21 was transformed by pGEX-4T-1 ligated with restriction DNA fragment cut out from pCR II plasmid vector of T-A clonig system. The growth of transformed BL21 was not different between the colony incubated at 37℃ for 24-48h and the colony stored at 4℃ for 7-10 days after 24h incubation at 37℃. The desirable OD(550) of culture medium for inducing the expression of fusion protein by isopropylthio-β-D-galactoside (IPTG) was from 0.6 to 1.0, because expression of native E.coli proteins per ml of culture medium was increased relatively when IPTG was added at OD(550) more than 1.0. The expression of fusion protein reached plateau around 6h after the induction. Relative expression of native E.coli proteins per ml of culture medium increased thereafter. Therefore, it may be desirable to purify the fusion protein around 6h after the induction. |
| Keywords | phosphacan (フォスファカン) glutathione S-transferase (グルタチオン-S-トランスフェラーゼ) BL21 IPTG fusion protein (融合蛋白) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 63 |
| End Page | 72 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313693 |
| JaLCDOI | 10.18926/11753 |
|---|---|
| Title Alternative | The effects of various fixating buffer soution in the electron microscopic observations |
| FullText URL | 006_055_061.pdf |
| Author | Akatsuka, Kazuya| |
| Abstract | 生物学的あるいは生化学的な緩衝液を使用して作製された電子顕微鏡試料と従来の電子顕微鏡用として使用されている緩衝液で作成した試料とで電子顕微鏡像から比較検討した。HEPES緩衝液で作製した試料の電子顕微鏡像は、従来の電子顕微鏡に用いられている緩衝液で作製した試料から得られた電子顕微鏡像と非常によく似た像を示し、このHEPES緩衝液は電子顕微鏡の固定用緩衝液として使用できることが認められたが、その他（PIPES、MOPS緩衝液）の緩衝液ではあまりにも細胞内の可溶性物質が溶出しすぎて使用に耐えなかった。電子顕微鏡学研究において使用されうる固定用緩衝液は細胞内のオルガネラの保持が余りにも良い場合には、その微細構造の解析が困難になり、程々に細胞内の可溶性物質が溶出した方が解析がし易くなると思われ、この様な観点から、今回使用したHEPES緩衝液は生物学的あるいは生化学的な研究から引き続き電子顕微鏡用の試料を作製する際、同じ緩衝液が使用できることが認められた。 |
| Keywords | 電子顕微鏡 (electron microscopy) 微細構造 (ultrastructure) 肝細胞 (liver cell) カコジル酸緩衝液 (cacodylate buffer) HEPES緩衝液 (HEPES buffer) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 55 |
| End Page | 61 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313640 |
| JaLCDOI | 10.18926/11750 |
|---|---|
| Title Alternative | Total Skin Electron Beam Therapy |
| FullText URL | 001_001_006.pdf |
| Author | Nakagiri, Yoshitada| Inamura, Keiji| Miyake, Hideaki| Tahara, Seiji| Mimura, Seiichi| Egusa, Tomomi| Mikami, Yasutaka| Yamada, Toshiharu| Sugita, Katsuhiko| Hiraki, Yoshio| |
| Abstract | The peripherally T-cell lymphoma; Mycosis fungoides etc, has the good radiation sensitivity, and has been adapted for total skin electron beam therapy. In this study the pendular irradiation method was used for the purpose of total skin electron beam therapy in Mycosis fungoides, and physical data on the radiation field and the electron beam energy were useful clinically. |
| Keywords | 電子線全身照射法 菌状息肉症 全身性皮膚疾患 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313887 |
