result 133 件
| Author | Kobayashi, J.| Morii, F.| Muramoto, S.| Nakashima, S.| |
|---|---|
| Published Date | 1976-02-28 |
| Publication Title | 農学研究 |
| Volume | volume55 |
| Issue | issue3 |
| Content Type | Departmental Bulletin Paper |
| Author | Nakashima, S.| Yagi, M.| Aoyama, I.| |
|---|---|
| Published Date | 1985-03 |
| Publication Title | 農学研究 |
| Volume | volume60 |
| Issue | issue4 |
| Content Type | Departmental Bulletin Paper |
| Author | Nisikado, Y.| Watanabe, K.| |
|---|---|
| Published Date | 1953 |
| Publication Title | Berichte des Ohara Instituts für landwirtschaftliche Forschungen |
| Volume | volume10 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| FullText URL | K0004670.pdf K0004670_honbun.pdf |
|---|---|
| Author | Fatmawati, Ni Nengah Dwi| |
| Published Date | 2012-12-31 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第4670号 |
| Granted Date | 2012-12-31 |
| Thesis Type | Doctor of Philosophy in Medical Science |
| Grantor | 岡山大学 |
| language | Japanese English |
| JaLCDOI | 10.18926/49526 |
|---|---|
| Title Alternative | Inference and Explanation: Textual Functions of Sentences with the Japanese modal marker ni chigai nai |
| FullText URL | hss_035_023_038.pdf |
| Author | Miyazaki, Kazuhito| |
| Publication Title | 岡山大学大学院社会文化科学研究科紀要 |
| Published Date | 2013-03-25 |
| Volume | volume35 |
| Start Page | 23 |
| End Page | 38 |
| ISSN | 1881-1671 |
| language | Japanese |
| Copyright Holders | Copyright © 2013 岡山大学大学院社会文化科学研究科 |
| File Version | publisher |
| NAID | 120005232464 |
| JaLCDOI | 10.18926/AMO/49252 |
|---|---|
| FullText URL | 67_1_9.pdf |
| Author | Fatmawati, Ni Nengah Dwi| Sakaguchi, Yoshihiko| Suzuki, Tomonori| Oda, Masataka| Shimizu, Kenta| Yamamoto, Yumiko| Sakurai, Jun| Matsushita, Osamu| Oguma, Keiji| |
| Abstract | Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival. |
| Keywords | botulinum phospholipase C botulinum toxin phospholipase C activity sphingomyelinase activity hemolytic activity |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-02 |
| Volume | volume67 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 9 |
| End Page | 18 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23439504 |
| Web of Science KeyUT | 000316829900002 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/49731 |
| Author | Gotoh, Kazuma| Kinumoto, Taro| Fujii, Eiji| Yamamoto, Aki| Hashimoto, Hideki| Ohkubo, Takahiro| Itadani, Atsushi| Kuroda, Yasushige| Ishida, Hiroyuki| |
|---|---|
| Published Date | 2011-04 |
| Publication Title | Carbon |
| Volume | volume49 |
| Issue | issue4 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/48565 |
|---|---|
| FullText URL | 66_3_253.pdf |
| Author | Zhang, Kai| Yamamoto, Yumiko| Suzuki, Tomonori| Yokota, Kenji| Ma, Shaobo| Nengah Dwi Fatmawati, Ni| Oguma, Keiji| |
| Abstract | Cultured Clostridium botulinum strains produce progenitor toxins designated as 12S, 16S, and 19S toxins. The 12S toxin consists of a neurotoxin (NTX, 7S) and a non-toxic non-hemagglutinin (NTNH). The 16S and 19S toxins are formed by conjugation of the 12S toxin with hemagglutinin (HA), and the 19S toxin is a dimer of the 16S toxin. Type A cultures produce all 3 of these progenitor toxins, while type E produces only the 12S toxin. The 7S toxin is cleaved into heavy (H) and light (L) chains by a protease(s) in some strains, and the H chain has 2 domains, the N-terminus (Hn) and C-terminus (Hc). It has been reported that type A toxins bind to the intestinal cells or cultured cells via either HA or Hc. In this study, we investigated the binding of type A and E toxins to Caco-2 cells using Western blot analysis. Both the type E 7S and 12S toxins bound to the cells, with the 7S toxin binding more strongly, whereas, in the type A strain, only the 16S/19S toxins showed obvious binding. Pre-incubation of the type E 7S toxin with IgG against recombinant type E Hc significantly inhibited the 7S toxin binding, indicating that Hc might be a main binding domain of the type E toxin. |
| Keywords | Clostridum botulinum neurotoxins Caco-2 binding Hc |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2012-06 |
| Volume | volume66 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 253 |
| End Page | 261 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 22729106 |
| Web of Science KeyUT | 000305669700009 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/48451 |
| Author | Kobayashi, Fumiaki| Aomine, Hiroki| Mizunashi, Wataru| Yu, Fujio| Tamura, Takashi| Inagaki, Kenji| |
|---|---|
| Published Date | 2012-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume101 |
| Content Type | Departmental Bulletin Paper |
| JaLCDOI | 10.18926/47181 |
|---|---|
| Title Alternative | Nouns in the Dative Case "ni" and the postposition "totte" in Japanese - Focusing on the combination with adjectives - |
| FullText URL | hss_032_197_212.pdf |
| Author | Yang, Jae Ran| |
| Keywords | ニ格名詞 後置詞 「とって」 形容詞 |
| Publication Title | 岡山大学大学院社会文化科学研究科紀要 |
| Published Date | 2011-11-25 |
| Volume | volume32 |
| Start Page | 197 |
| End Page | 212 |
| ISSN | 1881-1671 |
| language | Japanese |
| Copyright Holders | Copyright © 2011 岡山大学大学院社会文化科学研究科 |
| File Version | publisher |
| NAID | 40019151583 |
| Author | Hamada, Tojosuke| |
|---|---|
| Published Date | 1928-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume40 |
| Issue | issue9 |
| Content Type | Journal Article |
| Author | Uchida, Shigeo| |
|---|---|
| Published Date | 1930-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume42 |
| Issue | issue9 |
| Content Type | Journal Article |
| Author | Takahashi, Shinji| Shimizu, Akira| Kawashima, Takaharu| |
|---|---|
| Published Date | 2011-05 |
| Publication Title | 岡山実験動物研究会報 |
| Volume | volume27 |
| Content Type | Others |
| Author | Kaméyama, S.| Itano, S.| |
|---|---|
| Published Date | 1935-11-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume47 |
| Issue | issue11 |
| Content Type | Journal Article |
| Author | Miyasaki, Satoru| |
|---|---|
| Published Date | 1940-02-29 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume52 |
| Issue | issue2 |
| Content Type | Journal Article |
| Author | Sakida, Shinichi| Kuroda, Ryusuke| Benino, Yasuhiko| Nanba, Tokuro| |
|---|---|
| Published Date | 2009-12 |
| Publication Title | 環境制御 |
| Volume | volume31 |
| Content Type | Departmental Bulletin Paper |
| Author | Kitamura, Masahiko| |
|---|---|
| Published Date | 1959-06-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume71 |
| Issue | issue7-1 |
| Content Type | Journal Article |
| Author | Kitamura, Masahiko| |
|---|---|
| Published Date | 1959-06-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume71 |
| Issue | issue7-1 |
| Content Type | Journal Article |
| Author | Inada, Minoru| |
|---|---|
| Published Date | 1959-05-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume71 |
| Issue | issue6-1 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/40421 |
|---|---|
| Title Alternative | CHROMATOGRAPHIC ANALYSIS OF METALS BY ORGANIC REAGENTS (Ist Report) |
| FullText URL | pitsr_005_001_043.pdf |
| Author | Ashizawa, Takashi| |
| Abstract | The principle of this method is to combine the metals with organic reagents, then extract them with non-polar solvents, and determine them by making chromatograph with adequate adsorbents. Dithizone is used to determine Hg, Cd, Bi, Ag, Cu, Zn, Ni, Co, Tl, Pb, Pd and its isotopes, Pt group, Au, etc. Diphenylcarbazone is applicable to determine Hg, Cu, Zn, Ni, etc. Xanthate is useful to determine Mo, Cu, Ni, Co, etc. α-orβ- Nitrosoβ- orα-naphthol is used to determine Fe, Co, earth acids etc. Diethyldithiocarbamate is used to determine Mo, Cu, Ni, Co, Fe, etc. Oxine series are useful to determine Cu, Ni, Fe, V, rare earth etc. α-indolcarbonic acid is applied to determine Fe, Co, rare earth etc. Acethyl acetone is used to determine Th, Fe, etc. Some other organic reagents were used moreover. There are some elements of which necessary condition for quantitative determination is still not decided or the order of adsorption is indefmite. As adsorbents alkaline, neutral or acid alumina, diatom earth and aluminate, magnesium carbonate, calcium carbonate, urea formaline resin, heulandite, mordenite, desmine, chabazite etc· were used. With this method the author tryed severd determinations on rocks, minerals, mineral springs, organisms etc. The contento; of Cu, Zn, Ni and Co in rocks were 0.007, 0.008, 0.002 and 0.004% respectively. Cobalt content proved to be more than that of nickel. The mercury content of rocks was proved to be 10 times more to Clarke number, bismuth and cadmium content as same as or less than Clarke number. Silver in plants, soil and mineral waters, platinum in serpentine; mercury, cadmium, bismuth etc in mineral waters, trace heavy metals in organism, etc were determined. Radium B and Thorium B in hot spring waters were used as tracer. As organic solvents methyl-, ethyl-, butyl-, amy1-, octyl alcohol, dioxan, benzol, toluene, xylol, petroleum ether, ether, ethylacetate, chloloform, carbon tetrachloride, carbon disulfide etc were applied. |
| Publication Title | 岡山大学温泉研究所報告 |
| Published Date | 1951-08 |
| Volume | volume5 |
| Start Page | 1 |
| End Page | 43 |
| ISSN | 0369-7142 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/15296 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002509302 |
