| ID | 57901 |
| Sort Key | 3
|
| Title Alternative | Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
|
| FullText URL | |
| Author |
Kajiyama, Yuki
Graduate School of Environmental and Life Science, Okayama University
Mizobata, Satsuki
Graduate School of Environmental and Life Science, Okayama University
Akaji, Shusaku
Graduate School of Environmental and Life Science, Okayama University
Nemoto, Michiko
Graduate School of Environmental and Life Science, Okayama University
Tamura, Takashi
Graduate School of Environmental and Life Science, Okayama University
ORCID
Kaken ID
publons
researchmap
Inagaki, Kenji
Graduate School of Environmental and Life Science, Okayama University
Kaken ID
researchmap
|
| Abstract | Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
|
| 抄録（別言語） | , , ,
|
| Keywords | glycine oxidase
Marinomonas mediterranea
cysteine tryptophilquinone
recombinant expression
enzymatic glycine assay
|
| Note | 原著論文 (Original paper)
|
| Publication Title |
Scientific Reports of the Faculty of Agriculture, Okayama University
|
| Published Date | 2020-02-01
|
| Volume | volume109
|
| Publisher | 岡山大学農学部
|
| Publisher Alternative | The Faculty of Agriculture, Okayama University
|
| Start Page | 1
|
| End Page | 6
|
| ISSN | 2186-7755
|
| Content Type |
Departmental Bulletin Paper
|
| OAI-PMH Set |
岡山大学
|
| language |
Japanese
|
| File Version | publisher
|
| Eprints Journal Name | srfa
|
