Springer Science and Business Media LLC Acta Medica Okayama 0924-090X 114 8 2026 Basin boundary metamorphoses due to changes in accessible boundary orbits in passive dynamic walking 595 EN Kota Okamoto Department of Aeronautics and Astronautics, Graduate School of Engineering, Kyoto University Nozomi Akashi Graduate School of Informatics, Kyoto University Ippei Obayashi nterdisciplinary Education and Research Field, Okayama University Hiroshi Kokubu Department of Mathematics, Graduate School of Science, Kyoto University James A. Yorke Departments of Mathematics and Physics, Institute for Physical Science and Technology, University of Maryland Shinya Aoi Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, The University of Osaka Passive dynamic walking is a mechanical system that walks down a shallow slope without any input or control, and is a useful tool for understanding the dynamic properties of walking. This system has a wide variety of periodic solutions through bifurcations depending on the slope angle, resulting in chaotic attractors and fractal basin boundaries. In addition, basin boundary metamorphoses occur at certain slope angles, where the boundaries of the basin of attraction change abruptly, but the mechanism underlying this phenomenon remains largely unclear. A well-known dynamical system, the Hénon map, exhibits similar properties, and its basin boundary metamorphoses have been explained in terms of changes in accessible boundary orbits caused by intersections of manifolds associated with bifurcating solutions. Inspired by this framework, we propose a hypothesis for the mechanism of basin boundary metamorphoses in passive dynamic walking by introducing the concept of accessible boundary orbits and verify it numerically. Our results provide new insights into the governing dynamics of walking and contribute to a deeper understanding of nonlinear phenomena in locomotion systems. No potential conflict of interest relevant to this article was reported.

American Chemical Society (ACS) Acta Medica Okayama 1043-1802 37 3 2026 A Cysteine-Specific Cationization Strategy for Versatile Antibody Production against Intrinsically Disordered Proteins 580 589 EN Ryui Sakaguchi Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Ai Miyamoto Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Rikako Kutsuma Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Takeru Mori Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Daichi Nakashima Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Mirei Masui Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Tomoko Honjo Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Midori Futami Department of Bioscience, Faculty of Life Science, Okayama University of Science Mariko Morii Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Toshiyuki Oshiki Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University Junichiro Futami Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Several autoantigens relevant to the immune system, especially those targeted by autoantibodies induced by antitumor responses, tend to be rich in disordered regions and are prone to aggregation. This inherent instability presents significant challenges for the production, purification, and analysis of autoantigens in laboratory settings. Cysteine-specific cationization can effectively solubilize and purify these challenging proteins, allowing the isolation of full-length water-soluble antigens in their denatured state. The purified antigens enable accurate multiplex autoantibody assays using a suspension Luminex bead array platform. However, well-validated positive control antibodies are essential to ensuring precise clinical diagnosis. In this study, we prepared and characterized a panel of control antibodies by immunizing rabbits with cysteine-specific S-cationized antigens. The resulting antibodies predominantly recognized linear epitopes and were highly effective as quality control reagents in autoantibody array assays. Additionally, these antibodies maintained their ability to bind to their native, unmodified intracellular counterparts, highlighting the usefulness of this approach for producing antibodies against intrinsically disordered proteins. Although a modest immune response against the S-cationized modification site was observed, it remained minimal and did not affect the usefulness of the antibodies for assay validation. We propose this versatile cysteine-specific cationization platform for managing unstable proteins rich in disordered regions, supporting antigen production for diagnostics, and antibody development for research and validation purposes. No potential conflict of interest relevant to this article was reported.

Oxford University Press (OUP) Acta Medica Okayama 1467-5463 27 1 2026 SGCRNA: spectral clustering-guided co-expression network analysis without scale-free constraints for multi-omic data bbag021 EN Tatsunori Osone Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Tomoka Takao Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Shigeo Otake Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Takeshi Takarada Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Weighted gene co-expression network analysis (WGCNA) is among the most widely employed methods in bioinformatics. WGCNA enables the identification of gene clusters (modules) exhibiting correlated expression patterns, the association of these modules with traits, and the exploration of candidate biomarker genes by focusing on hub genes within the modules. WGCNA has been successfully applied in diverse biological contexts. However, conventional algorithms manifest three principal limitations: the assumption of scale-free topology, the requirement for parameter tuning, and the neglect of regression line slopes. These limitations are addressed by SGCRNA. SGCRNA provides Julia functions for the analysis of co-expression networks derived from various types of biological data, such as gene expression data. The Julia packages and their source code are freely available at https://github.com/C37H41N2O6/SGCRNAs.jl. No potential conflict of interest relevant to this article was reported.

岡山大学考古学研究室 Acta Medica Okayama 2026 岡山市向場・黒住丘陵の遺跡測量調査概要報告 EN 10.18926/70272 No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 1341-321X 32 3 2026 Tick-borne pathogens in ticks collected from Humans: A prospective clinical pilot study 102931 EN Shinnosuke Fukushima Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Takaomi Sumida Numakuma Hospital Osamu Kawamata Numakuma Hospital Yoshimi Hidani Numakuma Hospital Hideharu Hagiya Department of Infectious Diseases, Okayama University Hospital Tick-borne diseases (TBDs), particularly Japanese spotted fever, are an increasing public health concern in Japan. Data on pathogens carried by ticks removed directly from patients and their associated clinical outcomes remain limited. This prospective study investigated pathogen carriage in patient-derived ticks and evaluated the clinical risk of TBDs. Between April and October 2025, ticks were collected from patients presenting with tick bites at two medical institutions in Western Japan. Ticks were morphologically identified and spotted fever group rickettsiae (SFGR) was detected by nested PCR targeting the 17-kDa antigen gene, followed by sequence analysis. Clinical data, including patient background, antibiotic prescriptions, and outcomes, were reviewed. Clinical information was available for 70 patients (median age; 75 years), of whom 88.6% were prescribed prophylactic antibiotics. Ticks were collected from 60 patients (85.7%), and seven adults without antibiotic prophylaxis were followed for disease onset. Sixty-two ticks, predominantly Amblyomma testudinarium (88.7%), were analyzed. SFGR was detected in eight ticks (12.9%), including seven A. testudinarium and one Ixodes nipponensis, collected from seven patients. Two patients bitten by Rickettsia tamurae–carrying ticks were observed for one month without antibiotics and remained asymptomatic. In this prospective analysis, no clinically apparent rickettsiosis was observed following bites from R. tamurae–positive ticks without antibiotic prophylaxis; however, subclinical infection could not be excluded. Despite the small sample size, our findings suggest that the clinical risk associated with R. tamurae infection may be low. Direct analysis of removed ticks from patients may help characterize pathogen reservoirs and inform targeted approaches to TBDs. No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 2211-1247 45 1 2026 Immunopeptidomics combined with full-length transcriptomics uncovers diverse neoantigens 116781 EN Takamasa Ishino Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Tomofumi Watanabe Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Serina Tokita Division of Cancer Immunology, Graduate School of Medical and Dental Sciences, Niigata University Youki Ueda Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Katsushige Kawase Division of Cell Therapy, Chiba Cancer Center Research Institute Yuka Takano Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Yin Min Thu Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Yuta Suzuki Department of Computational Biology and Medical Sciences, The University of Tokyo Chie Owa Department of Computational Biology and Medical Sciences, The University of Tokyo Takashi Inozume Department of Dermatology, Chiba University Graduate School of Medicine Wenhao Zhou Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Joji Nagasaki Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Vitaly Kochin Department of Immunology, Nagoya University Graduate School of Medicine Toshihide Ueno Division of Cellular Signaling, National Cancer Center Research Institute Shinya Kojima Division of Cellular Signaling, National Cancer Center Research Institute Akiko Honobe-Tabuchi Department of Dermatology, University of Yamanashi Tatsuyoshi Kawamura Department of Dermatology, University of Yamanashi Takehiro Ohnuma Department of Dermatology, Kumamoto Kenhoku Hospital Takamitsu Matsuzawa Department of Dermatology, Chiba University Graduate School of Medicine Yu Kawahara Department of Dermatology, Chiba University Graduate School of Medicine Kazuo Yamashita KOTAI Biotechnologies, Inc Jason Lin Division of Cell Therapy, Chiba Cancer Center Research Institute Jun Koseki Division of Systems Biology, Nagoya University Graduate School of Medicine Hiroyoshi Nishikawa Department of Immunology, Nagoya University Graduate School of Medicine Motoo Araki Department of Urology, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Naoya Kato Department of Gastroenterology, Graduate School of Medicine, Chiba University Teppei Shimamura Division of Systems Biology, Nagoya University Graduate School of Medicine Shinichi Morishita Department of Computational Biology and Medical Sciences, The University of Tokyo Yutaka Suzuki Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo Hiroyuki Mano Division of Cellular Signaling, National Cancer Center Research Institute Toshihiko Torigoe Takayuki Kanaseki Division of Cancer Immunology, Graduate School of Medical and Dental Sciences, Niigata University Masahito Kawazu Division of Cell Therapy, Chiba Cancer Center Research Institute Yosuke Togashi Department of Tumor Microenvironment, Okayama University, Graduate School of Medicine Dentistry and Pharmaceutical Sciences Neoantigens are crucial for antitumor immunity and immune checkpoint inhibitor (ICI) efficacy by triggering strong immune responses. However, conventional methods for identifying neoantigens, such as whole-exon sequencing and short-read RNA sequencing (RNA-seq), appear to be insufficient, and the tumor mutational burden cannot sufficiently predict ICI efficacy. In this study, we employed a proteogenomic approach using long-read RNA-seq with Pacific Biosciences Single-Molecule Real-Time Sequencing technology to analyze full-length transcripts in combination with the human leukocyte antigen ligandome. As a result, many neoantigen candidates were identified, which were unregistered in a comprehensive database, including those from non-coding regions. Additionally, we validated the responses of specific T cell receptors (TCRs) to these candidates and identified several pairs of TCRs and neoantigens. These findings highlight the presence of more diverse neoantigens than expected that cannot be identified by conventional methods. No potential conflict of interest relevant to this article was reported.

SAGE Publications Acta Medica Okayama 0963-6897 35 2026 Addition of human platelet lysate to islet culture medium suppresses islet loss and improves transplantation outcomes EN Hirofumi Noguchi Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus Chika Miyagi-Shiohira Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus Takuya Sadahira Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Masami Watanabe Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Issei Saitoh Department of Pediatric Dentistry, Asahi University School of Dentistry No potential conflict of interest relevant to this article was reported.

Elsevier BV Acta Medica Okayama 0010-9452 194 2026 Increasing visual uncertainty modulates multisensory decision-making 50 62 EN Xiangfu Yang Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Weiping Yang Department of Psychology, Faculty of Education, Hubei University Yinghua Yu Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Yoshimichi Ejima Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Jiajia Yang Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University The brain integrates and transforms information from multiple senses to make optimal decisions, a process that is critical for navigating complex environments with perceptual uncertainty. Despite a growing consensus that individuals adapt flexibly to uncertain sensory input, whether increasing visual uncertainty influences the decision process itself or other, non-decision sensory processes during multisensory decision-making are unclear. Here, an audiovisual categorization task was used to examine the responses of human participants (N = 30) to visual and audiovisual stimuli under low-, medium-, and high-uncertainty conditions. Modeling the behavioral data using a drift‒diffusion model indicated that increased visual uncertainty in the audiovisual context decreased the evidence accumulation rate but had no effect on non-decision processes. Electrophysiological recordings confirmed and expanded upon these results: increased visual uncertainty in the audiovisual context reduced the amplitude during the late decision-making stage (300–380 msec) but had no effect on the amplitude during the early sensory encoding stage (140–220 msec). More importantly, electroencephalography analyses revealed that audiovisual integration in the early sensory encoding stage occurred robustly across all visual uncertainty conditions, whereas audiovisual integration in the late stage occurred only under medium and high visual uncertainty conditions. This study demonstrated that increased visual uncertainty modulates the decision process itself rather than early sensory encoding during multisensory decision-making. Moreover, multisensory integration strategies dynamically adapt to increasing visual uncertainty by engaging different mechanisms to maintain effective decision-making. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 LRP4 and Agrin Are Modulated by Cartilage Degeneration and Involved in β-Catenin Signaling in Human Articular Chondrocytes EN Shuichi NANIWA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Therapeutic effects of intracerebral transplantation of human modified bone marrow-derived stromal cells (SB623) with voluntary and forced exercise in a rat model of ischemic stroke EN Takayuki NAGASE Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 2731-0590 4 9 2025 Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC–CXCL1–CXCR2 axis 1135 1151 EN Jin Komuro Department of Cardiology, Keio University School of Medicine Hisayuki Hashimoto Department of Cardiology, Keio University School of Medicine Toshiomi Katsuki Department of Cardiology, Keio University School of Medicine Dai Kusumoto Department of Cardiology, Keio University School of Medicine Manami Katoh Department of Frontier Cardiovascular Science, Graduate School of Medicine Toshiyuki Ko Department of Frontier Cardiovascular Science, Graduate School of Medicine Masamichi Ito Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo Mikako Katagiri Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo Masayuki Kubota Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo Shintaro Yamada Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo Takahiro Nakamura Department of Cardiology, Keio University School of Medicine Yohei Akiba Department of Cardiology, Keio University School of Medicine Thukaa Kouka Department of Cardiology, Keio University School of Medicine Kaoruko Komuro Department of Cardiology, Keio University School of Medicine Mai Kimura Department of Cardiology, Keio University School of Medicine Shogo Ito Department of Cardiology, Keio University School of Medicine Seitaro Nomura Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo Issei Komuro Department of Frontier Cardiovascular Science, Graduate School of Medicine Keiichi Fukuda Department of Cardiology, Keio University School of Medicine Shinsuke Yuasa Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Masaki Ieda Department of Cardiology, Keio University School of Medicine Heart failure (HF) is a growing global health issue. While most studies focus on cardiomyocytes, here we highlight the role of cardiac fibroblasts (CFs) in HF. Single-cell RNA sequencing of mouse hearts under pressure overload identified six CF subclusters, with one specific to the HF stage. This HF-specific CF population highly expresses the transcription factor Myc. Deleting Myc in CFs improves cardiac function without reducing fibrosis. MYC directly regulates the expression of the chemokine CXCL1, which is elevated in HF-specific CFs and downregulated in Myc-deficient CFs. The CXCL1 receptor, CXCR2, is expressed in cardiomyocytes, and blocking the CXCL1–CXCR2 axis mitigates HF. CXCL1 impairs contractility in neonatal rat and human iPSC-derived cardiomyocytes. Human CFs from failing hearts also express MYC and CXCL1, unlike those from controls. These findings reveal that HF-specific CFs contribute to HF via the MYC–CXCL1–CXCR2 pathway, offering a promising therapeutic target beyond cardiomyocytes. No potential conflict of interest relevant to this article was reported.

Wiley Acta Medica Okayama 1752-8054 18 8 2025 Cardiotoxicity Assessment of EGFR Tyrosine Kinase Inhibitors Using Human iPS Cell‐Derived Cardiomyocytes and FDA Adverse Events Reporting System e70325 EN Shota Yanagida Division of Pharmacology, National Institute of Health Sciences (NIHS) Hiroyuki Kawagishi Division of Pharmacology, National Institute of Health Sciences (NIHS) Mitsuo Saito Japan Pharmaceutical Information Center (JAPIC) Hirofumi Hamano Department of Pharmacy, Okayama University Hospital Yoshito Zamami Department of Pharmacy, Okayama University Hospital Yasunari Kanda Division of Pharmacology, National Institute of Health Sciences (NIHS) Recent advances in the development of anti-cancer drugs have contributed to prolonged survival of cancer patients. In contrast, drug-induced cardiotoxicity, particularly cardiac contractile dysfunction, is of growing concern in cancer treatment. Therefore, it is important to understand the risks of anti-cancer drug-induced cardiac contractile dysfunction in drug development. We have previously developed image-based motion analysis using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to assess the effect of drugs on contractility. However, the utility and predictive potential of image-based motion analysis using hiPSC-CMs for anti-cancer drug-induced cardiac contractile dysfunction have not been well understood. Here we focused on epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) and investigated the correlation between the hiPSC-CMs data and clinical signals of adverse events related to cardiac contractile dysfunction. We examined the effects of the four EGFR-TKIs, osimertinib, gefitinib, afatinib, and erlotinib, on the contractility of hiPSC-CMs using image-based motion analysis. We found that osimertinib decreased contraction velocity and deformation distance in a dose- and time-dependent manner, whereas gefitinib, afatinib, and erlotinib had little effect on these parameters. Next, we examined the real-world data of the EGFR-TKIs using FDA Adverse Event Reporting System (FAERS; JAPIC AERS). Only osimertinib showed significant clinical signals of adverse events related to cardiac contractile dysfunction. These data suggest that hiPSC-CM data correlate with clinical signals in FAERS analysis for four EGFR-TKIs. Thus, image-based motion analysis using hiPSC-CMs can be a useful platform for predicting the risk of anti-cancer drug-induced cardiac contractile dysfunction in patients. No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 2041-1723 15 1 2024 Neurotransmitter recognition by human vesicular monoamine transporter 2 7661 EN Dohyun Im Department of Cell Biology, Graduate School of Medicine, Kyoto University Mika Jormakka Department of Cell Biology, Graduate School of Medicine, Kyoto University Narinobu Juge Department of Genomics and Proteomics, Advanced Science Research Center, Okayama University Jun-ichi Kishikawa Department of Applied Biology, Kyoto Institute of Technology Takayuki Kato Institute for Protein Research, Osaka University Yukihiko Sugita Laboratory of Ultrastructural Virology, Institute for Life and Medical Sciences, Kyoto University Takeshi Noda Laboratory of Ultrastructural Virology, Institute for Life and Medical Sciences, Kyoto University Tomoko Uemura Department of Cell Biology, Graduate School of Medicine, Kyoto University Yuki Shiimura Department of Cell Biology, Graduate School of Medicine, Kyoto University Takaaki Miyaji Department of Genomics and Proteomics, Advanced Science Research Center, Okayama University Hidetsugu Asada Department of Cell Biology, Graduate School of Medicine, Kyoto University So Iwata Department of Cell Biology, Graduate School of Medicine, Kyoto University Human vesicular monoamine transporter 2 (VMAT2), a member of the SLC18 family, plays a crucial role in regulating neurotransmitters in the brain by facilitating their uptake and storage within vesicles, preparing them for exocytotic release. Because of its central role in neurotransmitter signalling and neuroprotection, VMAT2 is a target for neurodegenerative diseases and movement disorders, with its inhibitor being used as therapeutics. Despite the importance of VMAT2 in pharmacophysiology, the molecular basis of VMAT2-mediated neurotransmitter transport and its inhibition remains unclear. Here we show the cryo-electron microscopy structure of VMAT2 in the substrate-free state, in complex with the neurotransmitter dopamine, and in complex with the inhibitor tetrabenazine. In addition to these structural determinations, monoamine uptake assays, mutational studies, and pKa value predictions were performed to characterize the dynamic changes in VMAT2 structure. These results provide a structural basis for understanding VMAT2-mediated vesicular transport of neurotransmitters and a platform for modulation of current inhibitor design. No potential conflict of interest relevant to this article was reported.

Wiley Acta Medica Okayama 2045-7634 14 15 2025 Real‐World Data of Comprehensive Cancer Genomic Profiling Tests Performed in the Routine Clinical Setting in Sarcoma e71098 EN Eiji Nakata Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Daisuke Ennishi Center for Comprehensive Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Tatsunori Osone Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Kiichiro Ninomiya Center for Comprehensive Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Shuta Tomida Center for Comprehensive Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Takuto Itano Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Tomohiro Fujiwara Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Toshiyuki Kunisada Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Naoyuki Ida Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Hideki Yamamoto Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Mashu Futagawa Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Tatsunori Shimoi Department of Medical Oncology, National Cancer Center Hospital Hiroyuki Yanai Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Akira Hirasawa Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Shinichi Toyooka Center for Comprehensive Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Masahiro Tabata Center for Clinical Oncology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Toshifumi Ozaki Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Introduction: Next-generation sequencing-based comprehensive cancer genomic profiling (CGP) tests are beneficial for refining diagnosis and personalized treatment of various cancers. However, the clinical impact of CGP, as covered by public health insurance in the management of sarcomas, remains unknown. Especially, the data on the utility of the newly emerging dual DNA–RNA panel compared to the conventional DNA-only panel in clinical settings is lacking. Therefore, we evaluated the utility of CGP in routine clinical practice for sarcoma treatment.<br> Patients and Methods: In this study, three types of DNA panel and one DNA–RNA panel, reimbursed by Japanese public health insurance, were utilized. We detected oncogenic and druggable gene mutations and genotype-matched therapies.<br> Results: One hundred and thirty-six patients were included in this study. Based on the detection of highly histology-specific translocations in the sequencing results, 2.2% of patients were re-classified. In patients with translocation-related sarcomas, a DNA–RNA panel identified more histology-specific fusion genes than DNA panels (p = 0.0035). Specifically, 86.8% and 39.0% of patients had oncogenic and druggable genomic alterations, respectively. Of these, 9.6% underwent genotype-matched therapy, with a 36.3% response rate and an 81.8% disease control rate. Patients who were administered genomically matched therapy had better overall survival (OS) than those who did not in patients with metastatic or advanced sarcoma with no prior chemotherapy (3-year OS: 83.3% vs. 48.0%, p = 0.42). Patients with TP53 and RB1 mutations had worse OS than those without. Germline findings were detected in 11.0% of the patients, one of whom had a truly germline origin.<br> Conclusions: This study suggests that publicly reimbursed CGP tests, particularly the dual DNA–RNA panel, could be beneficial for refining diagnostic precision in selected sarcoma subtypes, treatment decisions, detecting the germline findings, and prognosis prediction in routine clinical settings for sarcoma. The implementation of genotype-matched therapies showed favorable clinical outcomes and improved the prognosis. No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 0028-0836 638 8049 2025 Immune evasion through mitochondrial transfer in the tumour microenvironment 225 236 EN Hideki Ikeda Division of Cell Therapy, Chiba Cancer Center Research Institute Katsushige Kawase Division of Cell Therapy, Chiba Cancer Center Research Institute Tatsuya Nishi Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Tomofumi Watanabe Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Keizo Takenaga Division of Innovative Cancer Therapeutics, Chiba Cancer Center Research Institute Takashi Inozume Division of Cell Therapy, Chiba Cancer Center Research Institute Takamasa Ishino Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Sho Aki Division of Nutriomics and Oncology, RCAST, The University of Tokyo Jason Lin Division of Cell Therapy, Chiba Cancer Center Research Institute Shusuke Kawashima Division of Cell Therapy, Chiba Cancer Center Research Institute, Chiba, Japan Department of Dermatology, Graduate School of Medicine, Chiba University Joji Nagasaki Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Youki Ueda Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Shinichiro Suzuki Department of Medical Oncology, Kindai University Faculty of Medicine Hideki Makinoshima Tsuruoka Metabolomics Laboratory, National Cancer Center Makiko Itami Department of Surgical Pathology, Chiba Cancer Center Yuki Nakamura Division of Cell Therapy, Chiba Cancer Center Research Institute Yasutoshi Tatsumi Division of Cell Therapy, Chiba Cancer Center Research Institute Yusuke Suenaga Laboratory of Evolutionary Oncology, Chiba Cancer Center Research Institute Takao Morinaga Division of Cell Therapy, Chiba Cancer Center Research Institute Akiko Honobe-Tabuchi Department of Dermatology, Faculty of Medicine, University of Yamanashi Takehiro Ohnuma Department of Dermatology, Faculty of Medicine, University of Yamanashi Tatsuyoshi Kawamura Department of Dermatology, Faculty of Medicine, University of Yamanashi Yoshiyasu Umeda Department of Skin Oncology/Dermatology, Saitama Medical University International Medical Center Yasuhiro Nakamura Department of Skin Oncology/Dermatology, Saitama Medical University International Medical Center Yukiko Kiniwa Department of Dermatology, Shinshu University School of Medicine Eiki Ichihara Department of Allergy and Respiratory Medicine, Okayama University Hospital Hidetoshi Hayashi Department of Medical Oncology, Kindai University Faculty of Medicine Jun-ichiro Ikeda Department of Diagnostic Pathology, Graduate School of Medicine, Chiba University Toyoyuki Hanazawa Department of Otorhinolaryngology/Head and Neck Surgery, Chiba University Graduate School of Medicine Shinichi Toyooka Department of General Thoracic Surgery and Endocrinological Surgery, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Hiroyuki Mano Division of Cellular Signalling, National Cancer Center Research Institute Takuji Suzuki Department of Respirology, Graduate School of Medicine, Chiba University Tsuyoshi Osawa Division of Nutriomics and Oncology, RCAST, The University of Tokyo Masahito Kawazu Division of Cell Therapy, Chiba Cancer Center Research Institute Yosuke Togashi Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Cancer cells in the tumour microenvironment use various mechanisms to evade the immune system, particularly T cell attack1. For example, metabolic reprogramming in the tumour microenvironment and mitochondrial dysfunction in tumour-infiltrating lymphocytes (TILs) impair antitumour immune responses2,3,4. However, detailed mechanisms of such processes remain unclear. Here we analyse clinical specimens and identify mitochondrial DNA (mtDNA) mutations in TILs that are shared with cancer cells. Moreover, mitochondria with mtDNA mutations from cancer cells are able to transfer to TILs. Typically, mitochondria in TILs readily undergo mitophagy through reactive oxygen species. However, mitochondria transferred from cancer cells do not undergo mitophagy, which we find is due to mitophagy-inhibitory molecules. These molecules attach to mitochondria and together are transferred to TILs, which results in homoplasmic replacement. T cells that acquire mtDNA mutations from cancer cells exhibit metabolic abnormalities and senescence, with defects in effector functions and memory formation. This in turn leads to impaired antitumour immunity both in vitro and in vivo. Accordingly, the presence of an mtDNA mutation in tumour tissue is a poor prognostic factor for immune checkpoint inhibitors in patients with melanoma or non-small-cell lung cancer. These findings reveal a previously unknown mechanism of cancer immune evasion through mitochondrial transfer and can contribute to the development of future cancer immunotherapies. No potential conflict of interest relevant to this article was reported.

Springer Science and Business Media LLC Acta Medica Okayama 2045-2322 15 1 2025 Autoantibody spark response predicts treatment outcome in patients receiving chemoradiation followed by durvalumab therapy 27502 EN Takeru Mori Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Mio Kitagawa Department of Radiology, Sapporo Medical University School of Medicine Tomokazu Hasegawa Department of Radiology, Sapporo Medical University School of Medicine Masanori Someya Department of Radiology, Sapporo Medical University School of Medicine Takaaki Tsuchiya Department of Radiology, Sapporo Medical University School of Medicine Toshio Gocho Department of Radiology, Sapporo Medical University School of Medicine Tomoko Honjo Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Mirei Date Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Mariko Morii Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Ai Miyamoto Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Junichiro Futami Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University The PACIFIC regimen, comprising chemoradiotherapy (CRT) followed by maintenance with the immune checkpoint inhibitor (ICI) durvalumab, has become the standard of care for patients with unresectable non-small cell lung cancer (NSCLC). Although ICI is used to prevent recurrence by targeting residual microtumors, biomarkers capable of monitoring immune activity during this phase remain lacking. Here, we evaluated whether temporal changes in serum autoantibody levels can predict treatment efficacy. This retrospective study included 20 patients with unresectable stage II or III NSCLC who received the PACIFIC regimen. Serum autoantibodies against 130 antigens were quantified before CRT, after CRT, and two weeks after the first ICI dose. The primary outcome was progression-free survival (PFS), and its association with autoantibody dynamics was examined. We observed an immediate and strong autoantibody response (spark response [SR]) after ICI initiation in patients with favorable treatment outcomes. Patients with SR and programmed death ligand 1 (PD-L1) expression ≥ 50% showed better PFS (two-year PFS; 72.9% vs. 18.2%, p = 0.0021). These findings suggest that serial monitoring of serum autoantibodies can provide a noninvasive approach to assess immune activity and predict treatment outcomes in patients receiving CRT or ICI therapy. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Study of the Brain Topological Processing Mechanisms in the Human Sensorimotor System EN CHENYU WANG Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Human heart‑on‑a‑chip microphysiological system comprising endothelial cells, fibroblasts, and iPSC‑derived cardiomyocytes EN YUN LIU Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Inhibitory Effect of a Tankyrase Inhibitor on Mechanical Stress-Induced Protease Expression in Human Articular Chondrocytes EN Yoshifumi HOTTA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Photoinitiators Induce Histamine Production in Human Mast Cells EN Taro MIURA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2025 Human Cord Blood–Endothelial Progenitor Cells Alleviate Intimal Hyperplasia of Arterial Damage in a Rat Stroke Model EN HONGMING SUN Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 ジェネリック医薬品の効率的開発に資するヒト経口投与後血漿中濃度推移予測システムDissolution-Absorption Prediction (DAP) workflowの構築 EN Motoki ONISHI Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 Near-infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Patient-Derived Xenografts Using a Humanized Anti-Fibroblast Activation Protein Antibody EN Teruki KOBAYASHI Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 ヒト未成熟GV期卵の有用性に関する研究 EN Hayato ASAMA Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 ヒトにおける安静時唾液の性状と味覚感受性との関連 EN Aiko HYODO Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 ヒト長鎖ノンコーディングRNAの破骨細胞分化・機能への影響 EN Yuki NAMBA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism EN Yurika SHIMIZU Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 Interaction of Orexin and Bone Morphogenetic Proteins in Steroidogenesis by Human Adrenocortical Cells EN Yoshiaki SOEJIMA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2024 Aggregated chromosomes/chromatin transfer: a novel approach for mitochondrial replacement with minimal mitochondrial carryover: the implications of mouse experiments for human aggregated chromosome transfer EN Ryota OKAMOTO Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Synergistic therapeutic effects of intracerebral transplantation of human modified bone marrow-derived stromal cells (SB623) and voluntary exercise with running wheel in a rat model of ischemic stroke EN Satoru YABUNO Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Transplantation of modified human bone marrow-derived stromal cells affords therapeutic effects on cerebral ischemia in rats EN Satoshi KAWAUCHI Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2005 Development of a novel DDS vector : specific delivery of proteins to human hepatocytes with engineered bio-nanocapsule EN Dongwei Yu No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2005 ヒトヘルペスウイルス7型のDNAポリメラーゼサブユニットに対する単クローン抗体の性状と有用性 EN No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2005 Emergence of nuclear heparanase induces differentiation of human mammary cancer cells EN Tetsuji Nobuhisa No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 ヒトiPS細胞由来心筋細胞を用いた慢性収縮毒性評価法の開発 EN Shota YANAGIDA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Clinicopathologic Analysis of Sinonasal Inverted Papilloma, with Focus on Human Papillomavirus Infection Status EN Munechika TSUMURA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2023 Prevalence of Gender Dysphoria by Gender and Age in Japan: A Population-Based Internet Survey Using the Utrecht Gender Dysphoria Scale EN Yoshitaka OSHIMA Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 Meta-Analysis-Assisted Detection of Gravity-Sensitive Genes in Human Vascular Endothelial Cells EN YIN LIANG Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 A study on monomial ideals and Specht ideals EN Kosuke Shibata Graduate School of Natural Science and Technology, Okayama university No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 Anti-high mobility group box 1 monoclonal antibody suppressed hyper-permeability and cytokine production in human pulmonary endothelial cells infected with influenza A virus EN Takahiro Nanba Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 The resolution of immunofluorescent pathological images affects diagnosis for not only artificial intelligence but also human EN Kensaku Takahashi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 Adipose-Derived Extract Suppresses IL-1β-Induced Inflammatory Signaling Pathways in Human Chondrocytes and Ameliorates the Cartilage Destruction of Experimental Osteoarthritis in Rats EN Hideki Ohashi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2022 Overexpression of Adenovirus E1A Reverses Transforming Growth Factor-β-induced Epithelial-mesenchymal Transition in Human Esophageal Cancer Cells EN Tomoya Masuda Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 133 3 2021 間葉系幹細胞の細胞生物学的特徴づけと，ヒト多能性幹細胞研究への応用 158 165 EN Takeshi Takarada Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Lack of collagen α6(IV) chain in mice does not cause severe-to-profound hearing loss or cochlear malformation, a distinct phenotype from nonsyndromic hearing loss with COL4A6 missense mutation EN Shaoying Tang Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 EG-VEGF induces Invasion of a Human Trophoblast Cell Line via PROKR2 EN Kazumasa Tani Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 133 2 2021 頭頸部扁平上皮癌のgenomics 99 103 EN Mizuo Ando Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 In vitroデータに基づく代謝消失型化合物のヒト体内動態予測法に関する研究：生理学的薬物速度論におけるアルブミン媒介性肝移行の意義及びヒトiPS細胞由来小腸細胞の透過特性の応用 EN Kei Mayumi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Comparison of the Hybrid Capture II Method with a PCR-Based Screening Method Using a Carboxyfluorescein-Labeled Primer for Detecting Human Papillomavirus in Cervicovaginal Liquid-Based Cytology EN Yusuke Saiki Graduate School of Health Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Porphyromonas gulae proteases influence not only bacterial growth, coaggregation, and hemagglutination but also the maintenance of human protein EN Urmi Saki Alam Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 PRRX1 promotes malignant properties in human osteosarcoma EN Ryoji Joko Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2021 Histidine-rich glycoprotein stimulates human neutrophil phagocytosis and prolongs survival through CLEC1A EN Yohei Takahashi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 ヒト培養細胞におけるタンパク質発現限界を体系的に測定する実験方法の開発 EN Graduate School of Natural Science and Technology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 KCNJ13Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells EN Yuki Kanzaki Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 Oncolytic Virus-Mediated Targeting of the ERK Signaling Pathway Inhibits Invasive Propensity in Human Pancreatic Cancer EN Takeshi Koujima Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学 資源植物科学研究所 野生植物グループ Acta Medica Okayama ヒトタバメヒシバ EN No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 長鎖（約6 kb）lssODNおよびCRISPR / Cas9を用いたヒト科霊長類特異的lncRNAのマウス受精卵へのエレクトロポレーションによるノックイン EN Hoshi Kondo Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 機械的伸展刺激がヒト歯肉線維芽細胞と共培養したヒトiPS細胞由来心筋細胞の成熟に与える影響 EN Harumi Idei Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2020 DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation EN Yu Nomura Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 アルツハイマー型認知症患者の腸内細菌叢によるヒト型モデルマウスの構築とこの構築マウスの認知行動に腸内環境が及ぼす影響 EN Yusuke Fujii Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells EN Yuri Kunitomo Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学文学部 Acta Medica Okayama 2019 ヒトによる道具製作とジェンダー : 『線具』としてのヒモへの注目から見えること 26 35 EN Ayami Nakatani No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 周期的伸展刺激と静水圧刺激に対するヒト歯根膜細胞の形態と配向の変化 EN Ayano Fujita Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2019 歯周病原細菌を原因とするヒトと伴侶動物の犬における人獣共通感染症検査に関する研究 EN Masako Tai Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Synergistic antiproliferative effects of benzyl isothiocyanate in combination with methyl-β-cyclodextrin and MK571 in human colorectal cancer cells EN Qifu Yang Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Conclusive Evidence for OCT4 Transcription in Human Cancer Cell Lines: Possible Role of a Small OCT4-Positive Cancer Cell Population EN Tomoyuki Miyamoto Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Human Risk Assessment for Rat Liver Tumors Induced by a Constitutive Androstane Receptor Activator Momfluorothrin EN Yu Okuda Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 ヒト歯肉線維芽細胞との共培養がiPS細胞の心筋細胞への成熟に与える影響 EN Yusuke Matsuda Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 Physiological role of urothelial cancer-associated 1 long noncoding RNA in human skeletogenic cell differentiation EN Takanori Ishikawa Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2018 真菌二次代謝産物(+)-terreinがAggregatibacter actinomycetemcomitans刺激時にヒト歯肉上皮細胞に及ぼす影響の解明 EN Arisa Nakamura Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Inhibitory effect of JAK inhibitor on mechanical stress-induced protease expression by human articular chondrocytes EN Takahiro Machida Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 107 2018 アルツハイマー病の背景と発症 : ヒトマイクロバイオーム（細菌叢）との関連の視点から 5 10 EN Yusuke Fujii Hidetoshi Morita Alzheimer’s disease (AD) is a progressive, neurodegenerative disease characterized by memory and language disorder. The accumulation of senile plaques called β-amyloid and neurofibrillary tangles involving protein tau in the brains of AD patients have been considered as two hallmarks of AD. In AD, it is reported that accumulation of β-amyloid may be observed 25 years before onset, supporting early diagnosis and treatment by brain image analysis, because several techniques have recently been developed to detect β-amyloid and tau protein in brains of persons diagnosed with AD. AD patients are usually suffering from other diseases such as diabetes or periodontal disease, and there is accumulating data to show that these diseases associate with the human microbiome, such as gut and oral microbiota. In this report, the relation ship between AD and the human microbiome is reviewed. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Involvement of phosphatidylinositide 3-kinase pathway in the resistant mechanisms against benzyl isothiocyanate in human colorectal cancer cells EN Xiaoyang Liu Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Low prevalence of human mammary tumor virus (HMTV) in breast cancer patients from Myanmar EN Thar Htet San Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Immunohistochemistry of Vasohibin-2 in Human Kidney Disease: Implication in Impaired Glucose Tolerance and Reduced Renal Function EN Yuka Arata Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Overexpression of REIC/Dkk-3 suppresses the expression of CD147 and inhibits the proliferation of human bladder cancer cells EN Yuhei Horikawa Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 ヒト体外受精における単一凍結融解胚盤胞移植に関する研究 EN Satoshi Ueno Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 fMRI Studies on the Human Visual Object Perception in a Wide Visual Field EN Graduate School of Natural Science and Tchnology, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 ブタおよびヒト小卵胞由来卵母細胞の体外成熟能改善に関する研究 EN Yuichi Okudaira Graduate School of Environmental and Life Science, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Tachykinin Receptor 3 Distribution in Human Oral Squamous Cell Carcinoma EN Kyoichi Obata Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 真菌二次代謝産物である(+)-terrein がヒト歯肉線維芽細胞におけるinterleukin-6 誘導性タンパク質の産生に及ぼす影響とその標的分子の解明 EN Satoshi Yamamoto Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 Monitoring of Human Herpesviruses-6 and -7 DNA in Saliva Samples During the Acute and Convalescent Phases of Exanthem Subitum EN Yuki Miyazaki Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2017 The Downregulation of the Expression of CD147 by Tumor Suppressor REIC/Dkk-3, and its Implication in Human Prostate Cancer Cell Growth Inhibition EN Akihiro Mori Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 Human RAD 17 Polymorphism at Codon 546 Is Associated with the Risk of Colorectal Cancer EN Yukiko Yasuda No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 Ablation of MCL1 expression by virally induced microRNA-29 reverses chemoresistance in human osteosarcomas EN Shuhei Osaki No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 Iron depletion-induced downregulation of N-cadherin expression inhibits invasive malignant phenotypes in human esophageal cancer EN Seishi Nishitani No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 薬物トランスポーターヒトMATE1の機能と分子機構に関する研究 EN Tatsuya Kawasaki No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2016 Mechanical Stretch on Human Skin Equivalents Increases the Epidermal Thickness and Develops the Basement Membrane EN Eijiro Tokuyama No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 Human collagen XV is a prominent histopathological component of sinusoidal capillarization in hepatocellular carcinogenesis EN Koji Kimura No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 ヒト黄体化顆粒層細胞における細胞生存性に及ぼすヒト絨毛性ゴナドトロピンの影響に関する研究 EN Rei Hirata No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 Inhibitory Effects of Edaravone, a Free Radical Scavenger, on Cytokine-induced　Hyperpermeability of Human Pulmonary Microvascular Endothelial Cells: A Comparison with Dexamethasone and Nitric Oxide Synthase Inhibitor EN Yukie Saito No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 Hyaluronan suppresses mechanical stress-induced expression of catabolic enzymes by human chondrocytes via inhibition of IL-1β production and subsequent NF-κB activation EN Masatsugu Ozawa No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 Surrounding Cells Affect Gene Expression Pattern of Human Beta-defensins in Squamous Cell Carcinoma In Vitro EN Saori Takaoka No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2015 ヒト歯肉線維芽細胞のリソソーム酵素カテプシンB，L分泌に対するIL-6が及ぼす影響に関する研究 EN Ayaka Goto No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2014 Human Rho guanine nucleotide exchange factor 11 gene is associated with schizophrenia in a Japanese population EN Yutaka Mizuki No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2014 Maintenance of Glucose-sensitive Insulin Secretion of Cryopreserved Human Islets with University of Wisconsin Solution and Ascorbic Acid-2 Glucoside EN Takashi Arata No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2014 Studies on Human Mechanism of Audiovisual Temporal Integration by Event-related Potential EN No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2014 Quantitation and Human Monocyte Cytotoxicity of the Polymerization Agent 1-Hydroxycyclohexyl Phenyl Ketone (Irgacure 184) from Three Brands of Aqueous Injection Solution EN Kazuhiko Yamaji No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2014 DNA methylation status of REIC/Dkk-3 gene in human malignancies EN Tatsuro Hayashi No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 fMRI Studies on the Human Brain Mechanism of Visual Object Perception EN Bin Wang No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Dual Programmed Cell Death Pathways Induced by p53 Transactivation Overcome Resistance to Oncolytic Adenovirus in Human Osteosarcoma Cells EN Jo Hasei No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Simple vitrification for small numbers of human spermatozoa involving clinical outcomes EN Yuji Endo No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 口唇腺を用いたシェーグレン症候群におけるヒトβディフェンシンの発現パターン分析 EN Seiko Takeda No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 ヒト歯肉線維芽細胞におけるIL-6および可溶型IL-6受容体誘導性angiogenin産生に血管新生阻害物質terreinが及ぼす効果に関する研究 EN Daisuke Yamamoto No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Methanobrevibacter oralis およびヒトのグループIIシャペロニンに対する免疫応答の解析 EN Kimito Hirai No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Histone deacetylase inhibitors suppress mechanical stress-induced expression of RUNX-2 and ADAMTS-5 through the inhibition of the MAPK signaling pathway in cultured human chondrocytes EN Taichi Saito No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2013 Improvement of the Efficacy of 5-aminolevulinic Acid-mediated Photodynamic Treatment in Human Oral Squamous Cell Carcinoma HSC-4 EN Masanao Yamamoto No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 Mechanism of resistance to trastuzumab and molecular sensitization via ADCC activation by exogenous expression of HER2-extracellular domain in human cancer cells EN Ryosuke Yoshida No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 124 3 2012 高分化型ヒト肝癌由来細胞株“HuH-7” 231 238 EN Hidekazu Nakabayashi Kazuhisa Taketa 高分化型ヒト肝癌由来細胞株“HuH-7”は，1982年にCancer Researchにその樹立を報告した．HuH-7は，当時の岡山大学医学部附属癌源研究施設病理部門（故佐藤二郎教授）の下で樹立し，これまで多くの研究分野で利用され，世界的に有名な肝癌細胞株となっている．本稿では，有用性の高い分化機能を有するヒト肝癌細胞株HuH-7について，肝細胞癌の腫瘍マーカーであるα-fetoprotein（AFP）を中心に，この細胞株を用いた研究分野に関する詳細を紹介する． No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 124 2 2012 テロメラーゼ依存性腫瘍融解ウイルス療法の骨・軟部肉腫に対する前臨床的検討 105 110 EN Tsuyoshi Sasaki Hiroshi Tazawa Jo Hasei Toshiyuki Kunisada Aki Yoshida Yuuri Hashimoto Shuya Yano Ryosuke Yoshida Futoshi Uno Shunsuke Kagawa Yuki Morimoto Yasuo Urata Toshiyoshi Fujiwara Toshifumi Ozaki 骨・軟部肉腫は, 一部に治療抵抗性で予後の悪い症例が存在するため, 新たな治療法の確立が重要な課題である.　我々は, 5型アデノウイルスを基本骨格として, テロメラーゼ活性に依存して増殖する腫瘍融解ウイルス（OBP-301）や, coxsackie and adenovirus receptor（CAR）陰性の腫瘍細胞に感染するファイバー改変型ウイルス（OBP-405）を用い, 骨・軟部肉腫細胞に対する抗腫瘍効果を検討した.　 　14種類の骨・軟部肉腫細胞株に対してOBP-301の細胞障害活性を検討し, 12種類の細胞株でOBP-301に感受性を認めた.　また, OBP-301の細胞障害活性はCARの発現と相関していた.　さらに, テロメラーゼ活性の低い細胞に対しても, 5型アデノウイルスの複製に必須のE1Aによりテロメラーゼ活性の増強効果がおこり, 強い抗腫瘍活性を示すことを明らかにした.　次に, 骨肉腫脛骨同所性移植動物モデルを作成しOBP-301を投与したところ, OBP-301投与群では対象群と比べて有意に腫瘍増殖を抑制した.　最後に, OBP-301に感受性を認めなかったCAR陰性細胞株に対してOBP-405を用いて検討し, OBP-405が有効に作用することを確認した.　 　OBP-301やOBP-405を用いたウイルス療法は, 骨・軟部肉腫に対する新たな治療法となる可能性がある.　 No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 ヒトUDP-グルクロン酸転移ヒトUDP-グルクロン酸転移酵素UGT2B7の触媒機能におけるN-結合型糖鎖修飾の役割 EN Kenjiro Nagaoka No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 化学物質の光毒性・光遺伝毒性試験における培養ヒトケラチノサイトの有用性 EN Mayumi Horinouchi No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 Stage-specific embryonic antigen-4を用いたヒト乳歯歯根膜幹細胞の同定 EN Hiroaki Fukushima No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 Eosinophil Cationic Proteinが正常ヒト線維芽細胞に及ぼす影響に関する研究 EN Takamaro Sato No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 活性型Matrix metalloproteinase-3がヒト単球系細胞株THP-1における可溶型インターロイキン-6受容体の産生に及ぼす影響に関する研究 EN Hiroya Kobayashi No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 Mechanical stretch increases CCN2/CTGF expression in anterior cruciate ligament-derived cells EN Yoshiaki Miyake No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 Different responses to 5-fluoraouracil in mutagenicity and gene expression between two human lymphoblastoid cell lines with or without TP53 mutation EN Hiroaki Oka No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2012 Novel animal glioma models that separately exhibit two different invasive and angiogenic phenotypes of human glioblastomas EN Satoshi Inoue No potential conflict of interest relevant to this article was reported.

社団法人日本腎臓学会 Acta Medica Okayama 0385-2385 32 4 1990 抗ヒト腎モノクローナル抗体を用いた尿中及び血中のGBM抗原の測定 第2編 365 372 EN Yasuaki Mino Using a monoclonal antibody (Mab-G3) recognizing glomerular basement membrane (GBM), we assayed GBM antigen (G3-Ag) in the urine and serum of renal disease patients by sandwich ELISA. The subjects included normal control (NOR), minimal change nephrotic syndrome (MCNS), IgA nephropathy (IgA), membranous nephropathy (MN), membranoproliferative glomerulonephritis (MPGN) and chronic renal failure (CRF). The urine and serum was used as the material. With urinary G3-Ag, there were no statistically significant differences among the NOR, MCNS, IgA, MN, MPGN and CRF groups. Although no correlation was observed with proteinuria, hematuria, serum creatinine, serum β2 microglobulin and urinary NAG, urinary G3-Ag showed a significant (p<0.05) increase in excretion in the group of progressive CRF patients with s-Cr more than 1.0 mg/dl/month as compared to the stationary CRF group with s-Cr<1.0 mg/dl/month. Serum G3-Ag showed lower values in almost all cases, and there were no significant differences among the renal disease groups. The above findings led us to believe that the assay of urinary G3-Ag was useful in determining the degree of GBM disorder. It was also presumed that assay of renal antigens in urine and serum with the respective anti-human renal monoclonal antibodies could be a new tool in diagnosing renal diseases. No potential conflict of interest relevant to this article was reported.

社団法人日本腎臓学会 Acta Medica Okayama 0385-2385 32 4 1990 抗ヒト腎モノクローナノレ抗体を用いた疾患腎における抗原性の変化の検討 第1編 353 364 EN Yasuaki Mino We produced 22 different kinds of monoclonal antibody (Mab) by immunizing mice with human GBM antigens. In these Mabs, Mab-G1 to G5 recognized only GBM in the glomerulas, Mab-E1 and E2 recognized only glomerular epithelial cells, and Mab-M1 to M4 recognized mainly mesangium. The reactions of these Mabs with known GBM antigens such as type IV collagen, fibronectin and laminin were negative by immunoblotting. Using Mab-G1, Mab-E1 and Mab-M1, changes in the antigenicity of antigens recognized by Mabs were examined on kidney sections from the patients with various renal diseases by the indirect immunofluorescence test. When Mab-G1 recognizing GBM was used, there was no particular change of anti-genicity in minimal change nephrotic syndrome (MCNS) and IgA nephropathy (IgA), whereas in membranous nephropathy (MN) thickened GBM was found to maintain anti-genicity and the region of deposits was observed as negative punched-out region. In type I and III of membranoproliferative glomerulonephritis (MPGN), GBM was observed only outside of subendothlial deposits without showing double contour. In type II MPGN, GBM showed a double linear pattern and antigenicity of GBM in regions of dense deposits was not detected. When Mab-E1 recognizing glomerular epithelial cells was used, there was no change of antigenicity in the renal diseases. Further, in crescentic glomerulone-phritis, the region of the cellular crescents was not stained, When Mab-M1 recognizing mesangium was used, extensive staining was observed in the increased mesangium in IgA, MPGN, and diabetic nephropathy. We feel that it is of significance in elucidating the pathogenesis of renal diseases to study the changes of glomerular antigenicity in diseased kidneys by using anti-human renal monoclonal antibodies. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2011 Gene Expression and Localization of High-mobility Group Box Chromosomal Protein-1 (HMGB-1) in Human Osteoarthritic Cartilage EN Chuji Terada No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2011 Dendritic cell subsets and immunological milieu in inflammatory human papilloma virus-related skin lesions EN Yumi Nakayama No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2011 Telomerase-Dependent Oncolytic Adenovirus Sensitizes Human Cancer Cells to Ionizing Radiation via Inhibition of DNA Repair Machinery EN Shinji Kuroda No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2011 Characteristics of CD133(+) human colon cancer SW620 cells EN Hironobu Kawamoto No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2011 Histological evaluation of human alveolar sockets treated with artificial bone substitute material(MASTERGRAFTⓇ Granules).-A preliminary study- EN Mari Wakimoto No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2011 Studies on the Human Visual Mechanism of Wide-field by Functional Magnetic Resonance Imaging EN No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2010 Preclinical Evaluation of Differentially Targeting Dual Virotherapy for Human Solid Cancer EN Ryo Sakai No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2010 Histamine inhibits adhesion molecule expression in human monocytes, induced by advanced glycation end products, during the mixed lymphocyte reaction EN Jiyong Zhang No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 6-2 1959 脳の遊離アミノ酸について(X) 成熟ヒト脳およびヒト胎児脳各部位における遊離アミノ酸およびその関連物質について 3187 3191 EN Nobuhiro Fukai イオン交換クロマトグラフイーをもちいて,妊娠5ケ月および8ケ月胎児脳の各部位ならびに18才結節性硬化症患者脳の前頭葉,腫瘍周辺の非結節性組織について遊離アミノ酸およびその関連物質を各々分離定量した. 1) 妊娠5ケ月なうびに8ケ月胎児脳と成熟ヒト脳を比較すると,胎児脳と生後ヒト脳との間にはその遊離アミノ酸量にかなり著明な差異を認める. 2) 胎児脳では,脳各部位ともに多少の例外はあるが,生育の段階につれてタウリン,ホスホエタノラミン,グリシン,アラニン等は減少し,グルタミン酸,アスパラギン酸, γ-アミノ酪酸等は増加する傾向を示している. 3) γ-アミノ酪酸は胎児脳のいずれでも,視床下部で最高値を示した. 4) 生後のヒト脳で多量に測定され得るシスタチオニンは胎児脳にはほとんど存在しない. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 4-1 1959 脳の遊離アミノ酸について (VII) イオン交換クロマトグラフイーによるヒト脳遊離アミノ酸の定量 1591 1594 EN Shigeru Yunoue By means of the ion-exchange chromatography free amino acids and their related compounds in the fresh brain tissue have been estimated. As for the test material, pieces of the cerebral cortex sectioned at the time of the surgical operation in the patients with cerebral tumor or with epilepsy are used. As the result we have obtained the following notworthy differences when compared with the results previously reported by us concerning several species of mammalians. They are: (1) In the human brain there is a conciderable amount of cystathionine. Namely, 19.4 to 43.2mg/100g wet weight, and this substance is far smaller or not recognizable in other animals; and (2) the amounts of γ-aminobutric acid and taurine are a good deal smaller than animals. Moreover, in general the pattern of the free amino acids in the human cerebral cortex may be said to resemble closely that in dog. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 7-8 1989 ヒト培養肝癌細胞の産生するHBs抗原の精製方法とその物理化学的特性について 687 698 EN Munehiro Oda HBs antigen was purified from the culture fluid of hepatoma huGK-14 cell line and its physico-chemical properties were studied. The purification consists of following steps: concentration of culture fluid by membrane filtration, affinity column chromatography (anti-HBs monoclonal antibody column and anti-human serum albumin antibody column), and ultracentrifugation (isopycnic centrifugation in CsCl density gradient and rate zonal centrifugation on sucrose gradient). Highly purified (purity>99%) HBs antigen was isolated with an overall yield of about 40%. The HBs antigen showed uniform spherical particles (diameter: 23.2±2.9nm) and had a specific gravity of 1.20g/cm3. The purified HBs antigen yielded, in SDS-PAGE (under reducing conditions), four protein bands with apparent molecular weights of 22,000 and 26,000 (the two major bands), and 44,000 and 47,000. The two proteins of molecular weights of 26,000 and 47,000 are likely to be glycosylated, as these were several fold reduced when the cells were cultured in the presence of Tunicamycin. Amino acid analysis, Edman degradation, carboxypeptidase digestion, and ultraviolet absorption spectrum indicated that the HBs antigen from hepatoma cells is very similar to that derived from human plasma. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 5-6 1989 ヒト骨・軟部腫瘍における優性および劣性癌遺伝子の変異に関する研究 589 602 EN Toshifumi Ozaki Osteosarcoma is a malignant bone tumor which usually occurs in the metaphysis of long bones in adolescents or young adults, but the mechanism of disease occurrence is unknown. To examine alterations in the dominant oncogenes in the osteosarcoma and other bone and soft tissue tumors, DNA extracted from 12 bone tumors, 12 soft part tumors and 2 cells lines was hybridized with c-oncogene (c-myc, c-sis, c-raf-1, c-fos, K-ras2, c-erbB, c-fms, c-fos). DNA extracted from the same samples was examined with Rb cDNA (p0.9R, p3.8R) probes, the retinoblastoma gene (Rb gene) localized at 13q14, to analize whether a recessive mutation is a target in osteosarcoma, and other bone and soft tissue tumors. Amplification of c-myc was observed in 3 cases from 7 osteosarcomas and abnormalities in structure of the Rb gene were found in 4 from 7 osteosarcomas, 2 from 4 MFH, and 1 from 2 Ewing's sarcomas. Both amplification of the c-myc and abnormalities of the Rb gene were observed in 3 osteosarcoma cases. The results indicated that both the Rb gene and c-myc oncogene may be involved in the initiation of osteosarcoma. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 5-6 1989 肺扁平上皮癌の治療に関する研究 第2編 ヒト肺扁平上皮癌細胞株を用いた温熱化学療法に関する検討 581 588 EN Takumi Seto The effectiveness of hyperthermia in combination with anticancer drugs on EBC-1 cell line established from a patient with pulmonary epidermoid carcinoma was investigated. Anticancer drugs tested in the present study were adriamycin, bleomycin, cisdichlorodiammineplatinum (II) and mitomycin C. EBC-1 cells were incubated with the drug for one hour at 37°C or at elevated temperature (41°C, 42°C and 43°C). The enhancement of cytotoxicity by hyperthermia was found in combination with all of the drugs. The degree of enhanced cytotoxicity was positively elated to the temperature in combinations with adriamycin and bleomycin. Especially, a synergic enhancement of cytotoxicity was found with the combination of hyperthermia and bleomycin. The present study showed that the combination of hyperthermia and anticancer drugs produced potential cytotoxicity on EBC-1 cells in vitro, and may provide useful information for the clinical trials in the future. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 5-6 1989 肺扁平上皮癌の治療に関する研究 第1編 ハムスター移植ヒト肺扁平上皮癌細胞株に対する各種制癌剤の感受性について 573 580 EN Takumi Seto Chemotherapy is the only therapeutic modality applicable to patients with advanced pulmonary epidermoid carcinoma (PEC). However, the results of chemotherapy to PEC remain unsatisfactory. It is very important to have an accurate knowledge of the sensitivity of anticancer drugs against PEC in order to establish a successful chemotherapy. The in vivo sensitivity of 12 anticancer drugs was investigated using PEC cell line (EBC-1) xenografts in hamsters. In the present study, adriamycin, ifosphamide, mitomycin C, methotrexate and cisdichlorodiammineplatinum (II) showed antitumor activity against EBC-1 cells, but the other 6 drugs (ACNU, bleomycin, 5-fluorouracil, neocarzinostatin, procarbazine and vincristine) have no antitumor activity against EBC-1 cells. These results suggest that combination chemotherapy with 3 or 4 drugs with antitumor activity in the present study may be effective to PEC. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 5-6 1989 リスザルレトロウイルスLTR関連ヒト遺伝子の分子クローニングと構造解析 557 571 EN Kazutaka Nagao Sequences related to the squirrel monkey retrovirus (SMRV) LTR were isolated from a genomic library of human DNA by screening under conditions of relaxed stringency. The probe contains several transcriptional signals and its regulatory sequences in SMRV-H LTR, such as the enhancer sequence, CAT box, TATA box, and polyadenylation signal. More than 50 positive signals were detected in 4.4×105 recombinant phages. The cloned human sequences named SRH strongly hybridized with SMRV-H LTR and some of them weakly hybridized with SMRV-H structural genes. In SRH-1, the region related to SMRV-H prt-pol-env was flanked by the two separately located sequences related to the LTR. SRH-3 has a solitary LTR-related region, whereas, in SRH-5, the LTR-related region was adjacent to the pol-env-related sequence. The reliability of these hybridization experiments was confirmed by reciprocal hybridization. The restriction enzyme cleavage maps of these clones were different from those of known human endogenous retroviruses. Alu sequences, a human highly repetitive sequences, were inserted to the LTR-and pol-env-related regions in SRH-5. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 5-6 1989 ヒト免疫不全ウイルス抗原の免疫電子顕微鏡的研究 459 471 EN Satoru Endo Immunoelectron microscopic observations of human immunodeficiency virus (HIV) antigens were made using the indirect peroxidase-labeled antibody method with monoclonal antibodies (MoAb) to the envelope glycoprotein (gp160/120), matrix protein (MA, p17), and capsid protein (CA, p24). Antigens in the virions and HIV-infected cells were detected in frozen-sectioned specimens. Positive immunoreaction of MoAb to gp160/120 was located on the viral envelope, plasma membrane, endoplasmic reticulum, and nuclear membrane. The reaction of MoAb to p17 was at the inner leaflet of the viral envelope and the nucleoid. The reaction of MoAb to p24 was localized on and around the viral core. In cell suspension, however, positive immunostaining was observed only on the surface of the viral envelope and plasma membrane with MoAb to gp160/120, and no positive staining was observed with MoAb to p17 and MoAb to p24. The reaction of HIV-infected cells with normal control serum and all of the reactions in uninfected cells with these MoAbs were negative. It appears that antibodies have difficulty penetrating into virions and cells in cell suspension. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 5-6 1989 ヒトゲノムDNA中のリスザルレトロウイルス関連遺伝子の検出 423 435 EN Hirohiko Asonuma Squirrel monkey retrovirus (SMRV) is an endogenous type D retrovirus of the squirrel monkey, a New World primate. Southern hybridization with cloned SMRV-H revealed that 3040 copies of SMRV proviral DNA are present in the squirrel monkey genome and the majority have almost the same physical map as that of the cloned SMRV-H. SMRV-related sequences in the human genome were sought using the same method with various cloned SMRV-H DNA fragments under conditions of relaxed stringency. The discrete restriction fragments were frequently detected in the DNA from normal humans with the LTR and parts of gag and env as probes. Since SMRV LTR has very little homology with the LTRs of other retroviruses, the fragments detected with the LTR probe were characterized as SMRV-related human sequences. SMRV LTR-related sequences were also detected in the African green monkey and chicken, but not in the salmon, mouse, or dog. In conclusion, SMRV-related sequences are present in human DNA, and some of them might represent endogenous retroviral sequences of human DNA. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 3-4 1989 ヒト骨・軟部腫瘍組織における癌遺伝子の解析 285 293 EN Hiroshi Sumii Hajime Inoue Shiro Ito Gozo Tanabe Hideo Takechi Shogo Ikeda Takuzo Oda The amplification and rearrangement of five cellular oncogenes (c-myc, c-K-ras, c-fos, c-raf-1, and N-myc) were studied by Southern hybridization in fourteen human bone and soft tissue tumors obtained at surgery. Amplification of c-myc was detected in the two of four osteosarcomas and one of two malignant fibrous histiocytomas. The c-myc genes in these tissues were amplified 4- to 8-fold in comparision with the placenta DNA. One of these osteosarcomas had 16- to 32-fold amplification of c-raf-1 gene without rearrangement. The clinical course of osteosarcoma and malignant fibrous histiocytoma with the amplified c-myc or c-raf-1 gene showed a rapid malignant progress with lung or bone metastasis. There appears to be a correlation between clinical prognosis and oncogene amplification. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 1-2 1989 肝動脈塞栓術施行或は非施行ヒト肝癌組織の初代培養と株化 131 139 EN Yasunori Kusaka Takayoshi Tokiwa Jiro Sato Hiroshi Tsuge Keisuke Hamasaki Hisashi Mimura Kunzo Orita Primary culture of human primary liver cancer tissues treated with or without trancatheter arterial embolization (TAE) was perfomed with the following results. 1. The yield and viability were very low in cells from primary liver cancer tissues dissociated with enzymes. 2. Epithelial-like cells were found from TAE-treated cancer tissues at a ratio of 1/8 in both monolayer and explant culture and from TAE-nontreated tissues at a ratio of 4/12 in monolayer culture and 3/12 in explant culture. The AFP-producing capahity of these epithelial-like cells has been maintained from one week to one month in culture. 3. Cells derived from two TAE-nontreated cancer tissues were subculturable. One was established as a cholangiocellular carcinoma cell line. 4. No heterotransplantability of primary cultured cancer cells into nude mice was found. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 101 1-2 1989 ラットDAB肝癌の免疫組織学的研究―レクチンおよびヒト由来の肝細胞膜抗体の反応性について― 97 105 EN Osamu Tomida Membrane changes of DAB hepatic carcinoma cells were examined immunohistochemically, using the binding activity of Arachis hypogaea agglutinin (PNA) and anti-liver membrane antibody (anti-LM) from patients with HBsAg-negative chronic active hepatitis. The hepatic carcinoma in rats was produced through DAB feedings for 3-5 months. As controls, non-cancerous liver tissues from rats fed DAB, and normal rat liver were used. In isolated calls of the hepatic carcinoma and control tissues, FITC-PNA binded in 10 of 11 cases of DAB hepatic carcinoma and in none of control cases (11 rats fed DAB and 2 normal rats). In tissue sections, similar results were obtained. Lens culnaris agglutinin did not show any difference in binding among the 3 groups. Two anti-LMs did not bind to the membranes of isolated calls in 3 of 10 DAB hepatic carcinomas, although the anti-LMs were bound in all of controls. These facts indicate that the transformation, DAB hepatic carcinoma cells is accompanied by changes in the cell membrane, and that PNA may be useful in the analysis of membrane changes in carcinogenesis. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 102 11-12 1990 Subrenal capsule assay法による制癌剤感受性試験に関する研究 第1編 Subrenal capsule assay法の基礎的検討 1287 1297 EN Atsuhiko Tada Experimental evaluation of a new chemosensitivity test using a subrenal capsule assays (SRC) was performed. The effects of various immunosuppressants-cyclosporin A (CSA), cyclophosphamide, whole body irradiation, and Bredinin were studied using human small cell lung cancer cell line (SBC-3) serially transplanted in nude mice. A significant degree of host cell infiltration was seen in tumor fragments implanted under the renal capsule of immunocompetent mice. However, treatment with immunosuppressants effectively suppressed the host immune reaction. The most effective immunosuppressant was CSA at 60mg/kg. We compared the antitumor activities of CDDP, MMC, VP-16, ADM, CPA, and VCR against SBC-3 using SRC and clonogenic assays. SRC was performed using mice administered CSA 60mg/kg. Chemotherapeutic agents (1/2 LD(10)) were administered on day 1 and antitumor activities were evaluated on day 6 after implantation. The results of the assays were well-correlated except with VCR. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 102 11-12 1990 緑膿菌に対するヒト型モノクローナル抗体の作成とその臨床応用の可能性 1267 1273 EN Youji Kobayashi A human monoclonal antibody (IgG) against Pseudomonas aeruginosa was established within 3 weeks after fusion of patient lymphocytes and human myeloma WIL2-86 cells. This method is useful in establishing human monoclonal antibodies for infectious bacteria of a variety of antigenicities and allows for use of such antibodies as immunotherapeutic drugs in chronically infected patients. The opsonic effect of the monoclonal antibody we established was 1.8-fold higher than that of commercially available immunoglobulin drugs. Since the established human monoclonal antibody recognizes serotype M, which has not yet been produced, the antibody may be used as a part of immunotherapeutic drug “cocktail”. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 102 9-10 1990 好塩基球の反応性に関する研究 第1編 抗原，抗ヒトIgE刺激時の好塩基球の経時的形態的変化とヒスタミン遊離 1119 1126 EN Saburo Nakagawa Morphological changes in human basophils from atopic asthmatics were observed in whole blood smears with respect to histamine release after stimulation with antigen or anti-IgE. Histamine was rapidly released and was accompanied by morphological changes in basophils upon stimulation with antigen and anti-IgE. A decreased number of basophils and morphological changes including increased cell diameters, increased ratios of the short to long axis diameters, and decreased intracellular granule counts were observed upon the release of histamine after antigen and anti-IgE stimulation. Antigen stimulus induced both more rapid histamine release and morphological changes than anti-IgE stimulation. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 102 9-10 1990 ヒト正常脳および脳腫瘍におけるS-100蛋白サブユニット(α鎖，β鎖)の局在に関する免疫組織化学的研究 1061 1074 EN Takashi Tamiya Immunohistochemical localization of α and β subunits of the S-100 protein in normal human brains and brain tumors was studied by the ABC method using mouse monoclonal antibodies against each subunit. In cerebral tissues, glial cells showed positive staining for both subunits, with some neurons positive only for the α subunit. In cerebellum, Purkinje cells and other neurons were negative for the α or β subunit, and Bergmann's cells were positive only for the β subunit. Low grade astrocytomas were strongly positive for the α and β subunits. In oligodendrogliomas and choroid plexus papillomas, the immunoreactivity of the β subunit was stronger than that of the α subunit. Medulloblastomas showed no positive staining for either subunit. Schwannomas were positive only for the β subunit. High grade astrocytomas, ependymomas and meningiomas manifested marked variability in the immunoreactivity for the α and β subunits of the S-100 protein. The evidence suggests that immunohistochemical examination of the α and β subunits of the S-100 protein in normal human brains and brain tumors may be useful in determining the histogenetic origin of brain tumors. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 102 5-6 1990 非上皮細胞様形態を呈するヒト肝細胞癌由来細胞株の樹立 593 601 EN Akira Endo A cell line, HuH-33, was established in vitro from a patient with hepatocellular carcinoma (HCC), who had been treated with some chemotherapeutic agents. The chromosome number was widely distributed in 6888. This cell line has grown slowly, exhibiting a doubling time of approximately 150h, was intransplantable into nude mice, and secreted alpha-fetoprotein, albumin, β2-microglobulin, ferritin, tissue peptide antigen and extracellular matrix materials. HuH-33 was polygonal or spindle shaped, but it was keratin-positive and desmosome-like structures were observed with transmission electron microscopy, suggesting an epithelial origin of HuH-33. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 102 3-4 1990 肺癌組織内ヒト成長ホルモンの免疫組織学的および臨床的検討 357 364 EN Eiji Kunikata The human growth hormone (hGH) in lung cancer was studied immunohistochemically in 160 patients by the PAP method. Thirty-four neoplasm specimens (21.3%) contained hGH-positive tumor cells. Positive reaction for hGH was shown in 19 of 64 squamous cell carcinomas, 13 of 48 adenocarcinomas, 2 of 5 carcinods, and none of 26 small cell carcinomas and 17 large cell carcinomas. The sensitivity for hilar type lung cancer was higher than that for peripheral type (p<0.05). Positive rates for hGH were not affected by age, sex, tumor size, lymph node involvement or postsurgical stage. The survival rate of hGH positive patients was not worse than that of hGH negative. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2010 Quality control and biological potency management of therapeutic recombinant human erythropoietin EN Shigehiro Yanagihara No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2010 Analysis of Human Oral Bacterial Communities in Health and Disease EN Amgad Refaat Ahmed Hassan Wael No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2010 Advanced glycation end products enhance monocyte activation during human mixed lymphocyte reaction EN Katsuhisa Ohashi No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2010 A simple biological imaging system for detecting viable human circulating tumor cells EN Toru Kojima No potential conflict of interest relevant to this article was reported.

岡山大学温泉研究所 Acta Medica Okayama 0369-7142 53 1983 Studies on the release of histamine from basophils 2. Histamine release induced by house dust extract and anti-IgE 29 33 EN Yoshiro Tanizaki Haruki Komagoe Michiyasu Sudo Masaaki Mifune Hiroshi Morinaga Hikaru Kitani Yoshinori Goda Shinya Tada Ikuro Kimura 10.18926/21119 IgE-mediated release of histamine from whole blood was examined in two healthy and four asthmatic subjects by dose-dependent fashion. The significantly increased amount of histamine was released from basophils of both healthy and asthmatic subjects by a limited concentration of anti-IgE. Antigen (house dust) caused histamine release only from basophils of asthmatics who are sensitive to house dust. Basophils from one patients with asthma released no significant amount of histamine by anti-IgE. No potential conflict of interest relevant to this article was reported.

岡山大学温泉研究所 Acta Medica Okayama 0369-7142 53 1983 Studies on the release of histamine from basophils 1. Determination of histamine from whole blood by an automated fluorometric histamine analysis system 23 28 EN Yoshiro Tanizaki Haruki Komagoe Michiyasu Sudo Masaaki Mifune Hiroshi Morinaga Jun Ohtani Hikaru Kitani Yoshinori Goda Shinya Tada Ikuro Kimura 10.18926/21118 Histamine released from whole blood was determined by an automated fiuorometric histamine analysis system. The increased release of histamine from basophils by anti-IgE was observed in ten healthy subjects and 12 extrinsic asthma patients, while the release in 11 intrinsic asthma patients was significantly less as compared to that in healthy and extrinsic asthma subjects. House dust extract caused a significant increase in the histamine release from basophils of the extrinsic asthma patients who are sensitive to house dust. It was concluded from this study that histamine released from basophils could be easily determined by an automated analysis system and that the method is useful for the diagnosis and study of allergy. No potential conflict of interest relevant to this article was reported.

岡山大学温泉研究所 Acta Medica Okayama 0369-7142 54 1984 Studies on the release of histamine from basophils. 3. Correlation between basophil reactivity to anti-IgE and blood eosinophilia 35 38 EN Yoshiro Tanizaki Haruki Komagoe Michiyasu Sudo Masaaki Mifune Hiroshi Morinaga Saburo Nakagawa Hikaru Kitani Ikuro Kimura 10.18926/21106 Histamine release from basophils induced by anti-IgE was examined in 14 asthmatic subjects with blood eosinophilia. The subjects were divided into two groups; group I (with blood eosinophilia, positive skin test and positive RAST to house dust) and group II (with blood eosinophilia, negative skin test to various allergens and negative RAST to house dust). 1. Serum IgE levels in group I were much higher than those in group II. 2. Maximum percent histamine release induced by anti-IgE was much higher in group I than in group II. 3. Dose-response curve of anti-IgE-induced histamine release in group I showed consistent increase as anti-IgE concentrations increased. while, dose-response curve in group II was very low. These findings suggested that blood eosinophilia in group I might be elicited relating to IgE-mediated reaction. However, mechanism causing eosinophilia in group II was not clear in the present study. No potential conflict of interest relevant to this article was reported.

岡山大学温泉研究所 Acta Medica Okayama 0369-7142 55 1984 Studies on the release of histamine from basophils 4. Difference between house dust- and Candida-induced secretion 7 10 EN Yoshiro Tanizaki Haruki Komagoe Michiyasu Sudo Hiroshi Morinaga Hikaru Kitani Saburo Nakagawa Ikuro Kimura 10.18926/21095 Histamine releasse from basophils induced by house dust and C. albicans was examined in 30 patients with bronchial asthma. House dust and C. albicans caused a significant amount of histamine release in subjects with a RAST score to corresponding allergen. A close correlation was found between house dust- and anti-IgE-induced histamine release. However, histamine release induced by C. albicans was considerably different from the release induced by anti-IgE. No potential conflict of interest relevant to this article was reported.

岡山大学温泉研究所 Acta Medica Okayama 0369-7142 56 1985 Studies on the release of histamine from basophils 5. Clinical evaluation 17 21 EN Yoshiro Tanizaki Haruki Komagoe Michiyasu Sudo Hiroshi Morinaga 10.18926/21083 過去3年間にわたり,気管支喘息患者末梢血好塩基球からのヒスタミン遊離について,ヒスタミン自動分析装置を用いて全血法により検討を加えてきた. 1. 抗ヒトIgE によるヒスタミン遊離は,症例間で著しい差がみられた. この際血清IgE値が高値を示す症例では全般的に高度なヒスタシソ遊離がみられたが,血清IgE値が正常かまたはむしろ低値を示す症例のヒスタミン遊離は,かなり高度なものから全くみられないものまでさまざまであった. 2.ハウスダストやカンジダなどの抗原物質によるヒスタミン遊離は,特異的IgE抗体依存性であり,抗体価が上昇するにつれヒスタミン遊離は高度となる傾向がみられた. しかし,ハウスダストとカンジダによるヒスタミン遊離には若干の差がみられた. すなわち,ハウスダストと抗ヒトIgE によるヒスタミン遊離の間には密接な関連がみられたが,カンジダと抗ヒトIgEの間には全く関連がみられなかった. またカンジダによるヒスタミン遊離においては,IgE系反応以外の反応が関与する可能性が一部示唆された. No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 10 1993 環境の発ガン物質に対するヒト曝露の研究 2 4 EN Hikoya Hayatsu No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 15 1998 癌の遺伝子治療 12 16 EN Noriaki Tanaka 癌抑制遺伝子p53の突然変異や欠損による機能的異常は､多くのヒト癌で普遍的かつ高頻度に認められている｡p53タンパク質の機能の一つとしては､細胞増殖に関するいろいろな遺伝子の発現制御を介した細胞周期の調節が考えられているが､その他に最近アポトーシスの誘導分子としても注目を浴びてきている｡正常型p53遺伝子を有する胃癌､大腸癌では､術前化学療法や放射線療法で癌細胞のアポトーシスが誘導されたが､変異型p53を発現する腫瘍 ではアポトーシスに陥った細胞はほとんど認められなかった｡ヌードマウスの皮下に移植したp53遺伝子に異常を持つヒト肺癌腫瘍にリコンビナント･アデノウイルスベクターを用いて正常型p53遺伝子を導入すると､抗癌剤に対する感受性が劇的に増強し､シスプラチンの腹腔内投与により腫瘍内にアポトーシスによる広範囲な組織破壊が認められた｡この正常型p53発現アデノウイルスベクターとDNA障害性抗癌剤を併用した遺伝子治療は､臨床的にヒト悪性腫癌に応用可能と考えられる｡ No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 21 2004 高脂肪･高カロリー飼料がMeg1/Grb10遺伝子導入マウスの糖尿病発症に及ぼす影響 35 37 EN Yoshie Yamamoto Osamu Suzuki Kozue Yamada-Uchio Tomoko Ishino-Kaneko Junichirou Matsuda Katsunori Sato Meg1/Grb10遺伝子導入マウスはインスリンのシグナル伝達阻害による高インスリン血症を呈することから､II型糖尿病モデルと考えられている｡そこで､本モデルマウスを用いてII型糖尿病発症に及ぼす飼料の影響について検討した結果､ヒトII型糖尿病モデルとして有用性を確認することが出来た｡ No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 24 2007 2型糖尿病モデル動物Meg1/Grb10遺伝子導入マウスの有用性の検討 - 血漿成分及び糖尿病関連遺伝子の発現と発症の解析 - 19 21 EN Yoshie Yamamoto Osamu Suzuki Kozue Yamada-Uchio Tomoko Ishino-Kaneko Junichirou Matsuda Katsunori Sato Meg1/Grb10遺伝子導入マウス（Meg1マウス）はインスリンのシグナル伝達阻害による高インスリン血症を呈することから2型糖尿病モデルと考えられている。Meg1マウスは肥満を伴なわずに高血糖を発症するが､脂肪･カロリーの過剰摂取によっても糖尿病の発症が著しく増加する｡本研究はMeg1マウスの2型糖尿病モデルとしての有用性を検討するために､Meg1マウスと対照マウスを高脂肪･高カロリー飼料（HFD）及び対照飼料（NFD）で飼育した時の血漿アディポネクチン量とBMI値を比較するとともに糖尿病関連遺伝子の発現量について他の糖尿病モデルマウスと比較検討した。血漿アディポネクチン量はMeg1マウスのHFDが最も高く､対照マウスのNFDが最も低い値を示した。一方､BMI値は対照マウスのHFDが最も高い値を示し､血漿アディポネクチン量とBMI値は逆相関が認められ､ヒト2型糖尿病と類似することが認められた。また､Grb10､Glut4遺伝子の発現量はMeg1マウスと他の糖尿病モデルマウスでは異なる値を示し､Meg1マウスでのGrb10遺伝子の発現量は高く､Glut4遺伝子の発現量は低かった。以上のことから､Meg1マウスには他の糖尿病モデルと異なる発症機構の存在が示唆され､Meg1マウスは2型糖尿病モデルとしての有用性が考えられた。 No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 68 9 1956 パラチオン分解酵素に関する研究 �U. ヒト血清のパラチオン分解能及びパラチオン分解酵素の諸臓器（イヌ）内分布に就て 1305 1308 EN Teiji Shiigi Human blood serum and various organs of dogs were determined for its content of parathion-splitting enzyme by the procedure presented in the preceding paper. Ten per cent saline suspension of the relevant homogenized tissues was used for the estimation of parathion-splitting enzyme of the organs. (1) The activity of the enzyme in blood serum of normal individuals was 134 ± 44.6 γ/ml for men and 180 ± 62 γ/ml for women. (2) Of the various organs of dogs the blood serum was the most abundant in the enzyme, and the liver, the lung, the bone marrow and the kidney followed in the order given. The distribution of the enzyme was not appreciable in other organs. (3) The blood, the liver, the lung and the bone marrow were thought to play a major part in the detoxication of parathion, because the afore-mentioned decomposition of this poison to the less toxic paranitrophenol was believed to imply detoxication. The blood will constitute the most important and the liver the next-important organ of detoxication, if volume of the organs is taken into account. No potential conflict of interest relevant to this article was reported.

岡山大学医学部附属環境病態研究施設, 岡山大学医学部附属病院三朝分院 Acta Medica Okayama 09133771 59 1988 Basophil histamine release by anti-IgE in Subjects of chronic bronchitis and bronchial asthma 31 36 EN Yoshiro Tanizaki Michiyasu Sudo Hikaru Kitani Hiroyuki Araki Mitsuaki Tsuji Kiyoshi Takahashi Ikuro Kimura 10.18926/19755 Histamine release from basophils induced by anti-IgE was studied in 8 patients with chronic bronchitis and 50 patients with bronchial asthma by analyzing doseresponse curves. As the result, there were no significant differences in maximum percent histamine release from basophils among three groups of healthy subjects (24.7± 14.2%), patients with chronic bronchitis (27.7±22.1%) and those with bronchial asthma (28.4±17.0%). In the patients with bronchial asthma, the maximum percent histamine release was higher in accordance with higher serum IgE levels, and low maximum percent release was observed in patients with intrinsic asthma (14.1±7.2%). Study of dose-response curves of anti-IgE-induced histamine release showed that a negative slope from E(2) to E(1) was observed in both healthy subjects and patients with chronic bronchitis. The majority of asthmatics with serum IgE levels of 501IU/ml or over showed a positive slope from E(2) to E(1). No potential conflict of interest relevant to this article was reported.

岡山大学医学部附属環境病態研究施設, 岡山大学医学部附属病院三朝分院 Acta Medica Okayama 09133771 58 1987 Terfenadineの抗アレルギー作用について 5 11 EN Yoshiro Tanizaki Haruki Komagoe Michiyasu Sudo Hikaru Kitani 10.18926/19710 選択的H(1)受容体拮抗薬であるTerfenadineの抗アレルギー作用，特にIgE系反応によるヒスタミン，およびCa ionophore A23187による好中球からのleukotrienes遊離に対する抑制作用を，ヒト末梢血白血球を用いて検討した。１．Terfenadineは，抗ヒトIgE刺激による健康人好塩基球および特異抗原刺激による気管支喘息患者好塩基球からのヒスタミン遊離に対して，全血法でのin vitro添加実験では有意の抑制作用を示さなかった。 2．洗浄白血球法によるin vitro添加実験では，Terfenadineは特異抗原および抗ヒトIgE刺激時の気管支喘息患者好塩基球からのヒスタミン，Ca ionophore　A23187刺激時のleukotriene B(4)遊離に対して有意の抑制効果を示した。3．Terfenadineの内服によるin vivoの抑制実験では，IgE系反応による健康人および気管支喘息患者好塩基球からのヒスタミン遊離に対して有意の抑制効果は見られなかったが，特異抗原による皮膚反応に対しては明らかな抑制作用が観察された。以上より，Terfenadineは，組織肥満細胞および組織へと遊走してきた好塩基球に対してはある程度の抑制作用を有しているものと考えられた。 No potential conflict of interest relevant to this article was reported.

岡山大学医学部附属環境病態研究施設, 岡山大学医学部附属病院三朝分院 Acta Medica Okayama 09133771 57 1986 Tranilast(Rizaben®)の抗アレルギー作用について 42 45 EN Yoshiro Tanizaki Haruki Komagoe Michiyasu Sudo Hiroshi Morinaga Jun Ohtani Ikuro Kimura 10.18926/19683 抗アレルギー剤（脱顆粒抑制剤）の1つであるtranilastの肥満細胞の遊離機序に対する抑制作用について検討を加えた。1．抗原刺激時の肥満細胞の(45)Ca uptakeおよびヒスタミン遊離に対して，tranilastは有意の抑制作用を示したが，comp.48/80刺激時にはtranilastの(45)Ca uptake，ヒスタミン遊離に対する抑制作用はほとんどみられなかった。2．phosphatidylserine添加時には，(45)Ca uptakeに対するtranilastの抑制作用は減弱傾向を示し，この傾向はヒスタミン遊離に対する作用に比べより高度であった。3．抗原および抗ヒトIgEによる好塩基球からのヒスタミン遊離に対して，tranilastは明らかな抑制作用を示さなかった。 No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 5-1 1959 ヒト胎児血清グロブリン中に含有する特異蛋白に関する研究 2293 2301 EN Yoshinori Miyoshi Yoshiyuki Ochiai 1) When rabbits are immunized by human fetal serum, high titer precipitin reaction on human serum can be obtained by injecting ten times. 2) When rabbits are immunized by human fetal serum with Globulin and Albumin precipitin productions can be obtained easier than that of human fetal serum. As for each antigenity, the remarkable difference can not be seen and the antigenity of fetal serum Albumin is by no means inferior to that of Globulin. 3) In case of fetal serum Albumin as antigen, precipitin quantity is lower than that of Globulin, and appearance of reaction is less. 4) Specific protein contained in fetal serum, exists in serum Globulin, not in Albumin. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 2-1 1959 ヒト胎便中に含有する特異蛋白の血清免疫学的研究 555 565 EN Yoshinori Miyoshi Author intended the sero-immunologic studies of human meconium as the series of studies concerning fetal serum, placenta and so on, and then obtained the results as follows; 1) When rabbits are injected repeatedly with the extract of human meconium, the antiserum can be obtained high titer precipitin reaction on human serum. 2) Researching production of precipitin about rabbit's serum immunized with human meconium of the 7, 8, 9 and 10th pregnant months separatedly, author cannot find striking differences on antigenity in each months. 3) Rabbit's serum immunized with human meconium has very strong species specifity. 4) In human meconium exist specific protein fractions which are not contained in the adult human serum and these fractions are considered to coincide with those exist in the fetal serum and there were very small quantities. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 70 7 1958 ヒト胎盤ならびに胎児血清蛋白による妊娠ウサギ臓器のアレルギー性組織変化について 第2編 ヒト胎児血清,胎盤ならびに羊水蛋白による妊娠ウサギ臓器のアレルギー性組織変化について 2415 2426 EN Yasumasa Okamura No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 70 1 1958 ヒト胎盤ならびに胎児血清蛋白による妊娠ウサギ臓器のアレルギー性組織変化について 97 103 EN Yasumasa Okamura It has been discovered that there exists, within the fetus serum, as well as human placenta, a similar specific protein that one cannot find out in any human serum; which has been considered to have been formulated in placenta; further, one can probe out the abovesaid unique protein part in a show alike. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 電気刺戟（in vitro）の脳物質代謝におよぼす影響 第3編 電気刺戟（in vitro）の大脳トランスアミネーションにおよぼす影響 1463 1469 EN Hisashi Kumashiro 1) ダイコクネズミ,タイワンザル,ヒト脳の大脳ホモジネートおよび大脳皮質切片を用いて電気刺戟(in vitro)のトランスアミネーションにおよぼす影響をみた. 2) ダイコクネズミ大脳においてはAsGT活性は電気刺戟によつてホモジネートでは14.6%切片では9.6%阻害された.すなわちホモジネートの方が切片より阻害率は著明なようであつた.またAIGT活性は電気刺戟によつてホモジネート,切片とも著変なく,γAGT活性は電気刺戟によつてホモジネート,切片とも阻害傾向を示した.そこでAIGTは酵素系が異なるためではないかと推論した.また反応率はいづれの場合も切片の方がホモジネートより減少していた. 3) タイワンザル大脳皮質ホモジネートにおいてはAsGT活性は電気刺戟によつて平均約11%阻害された.またヒト健常大脳皮質切片においてはAsGT活性は電気刺戟によつて平均約11.3%の促進をみ,ヒト萎縮大脳皮質ホモジネートにおいては殆ど変化をみなかつた.また反応率を比較するとヒト健常大脳皮質切片ではダイコクネズミの切片の場合に比し対照時,刺戟時ともに約20%高く,ヒト萎縮大脳皮質ホモジネートではダイコクネズミのそれに比しやや低い値を示した.すなわち萎縮脳のAsGT活性の低下が考えられる. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 75 10 1963 脾組織培養法による脾内巨核球に関する研究 第3編 ヒト脾内巨核球について 903 912 EN Atsumasa Hanzawa Followings are the results of studies by our clinical tissue culture method mainly on motility and ability of platelet formation of megakaryocytes in the spleen of human embryo and a patient with osteomyelofibrosis. 1) Megakaryocytes are always found in the spleen of human embryo, and no significant morphological differences are noted from those in the bone marrow. 2) Deformated movement, pseudopodial movement, and tentacle formation for the platelet formation are noted on megakaryocytes in the spleen of human embryos. As for functions of megakaryocytes, motility and ability of the platelet formation are significantly found at 4 month of age, but are remarkably decreased after 5 month of age. 3) A correlation is noted in human embryo between the functions of megakaryocytes and erythropoiesis in the spleen, a rise and fall of the one being accompanied by the other. 4) In a patient with osteomyelofibrosis, megakaryocytes found in the spleen show a remarkable decrease in the functions. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 脳の遊離アミノ酸について(XIII) ヒト脳各部位の遊離アミノ酸およびその関連物質 1299 1306 EN Hirosuke Nishioka By means of ion exchange column chromatography the author carried out quantitative analyses of 14 kinds of free amino acids including the related compounds using the brain of the person who died of acute loss of blood. The parts of the brain used for the analysis were frontal cortex, corpus callosum, caudate nucleus, globus pallidus, thalamus, hypothalamus and medulla oblongata. The results are as follows. 1. In corpus callosum which contains little cellular components extremely minute quantities of phosphoethanolamine, aspartic acid, glutamic acid and γ-aminobutyric acid could be detected. 2. In globus pallidus a surprisingly large quantity of γ-aminobutyric acid could be found and it was far greater than that contained in hypothalamus. 3. In medulla oblongata only small quantities of phosphoethanolamine, aspartic acid, and glutamic acid could be detected. Likewise γ-aminobutyric acid was found not so abundant. This seems to be due to the fact that the present experiment was conducted with medulla oblongata including white matter. 4. Although only in a small quantity, cystathionine could be assayed in all these parts except globus pallidus and thalamus. 5. Even from the comparative biochemistry the present quantitative analyses gave an interesting contrast to the values obtainable in the brains of lower animals. 6. Although it was difficult to recognize any distinct difference in the pattern of amino acids between the adult brain and the infant brain, there was a clear-cut difference in the amino acid pattern of the adult brain and that of the fetal brain. Namely, in the adult human brain there exist far greater quantities of aspartic acid, glutamic acid, γ-aminobutyrie acid, and N-acetylaspartic acid than those in the fetal brain and conversely far less quantities of phosphoethanolamine and taurine than in the latter. Likewise tyrosine detected in the fetal brain could not be recognized in the adult human brain. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 2 1960 脳のトランスアミナーゼ 第1編 タイワンザル脳およびヒト脳におけるトランスアミナーゼ活性について 507 513 EN Masaya Ono タイワンザル脳，事故死ヒト脳の大脳皮質，大脳白質および小脳皮質，さらに高度の脳萎縮を伴い老人性痴呆にて死亡したヒト脳の大脳皮質を用い， 11種のα-アミノ酸， 3種のω-アミノ酸， 3種のヂアミノ酸，システイン酸，タウリンについて，組織のトランスアミナーゼ活性を測定した. 1. タイワンザル脳では，大脳皮質においてアラニンとバリンの活性が他部より低く，γ-アミノ酪酸とγ-アミノ-β-オキシ酪酸が高い. 2. 健常人脳では，アスパラギン酸，セリンが大脳皮質に，ヒスチジン，ロイシン，バリンが小脳皮質にて他部より高く，アラニン，フエニールアラニン，システイン酸は大脳白質において低い活性を示した. 3. タイワンザルおよび健常ヒト脳の大脳皮質では，いずれもアスパラギン酸，グリシン，フエニールアラニン，オルニチン，システイン酸の高い活性，およびアラニンの低い活性の点で他の下等動物脳と異なる. 4. 健常ヒト大脳皮質は，バリン，ロイシンおよび3種のω-アミノ酸の低い活性を以つてサルおよび下等動物と異なる. 5. 萎縮したヒト大脳皮質は，アラニン，ロイシン，バリン，γ-アミノ-β-オキシ酪酸の活性が健常ヒト大脳皮質より高く，むしろ下等動物のそれに近い.ヒスチジン，プロリン，オルニチンのグルタミン酸生成の著しい増加は健常ヒト脳および下等動物脳のいずれとも異なるが，ヒスチジンとプロリンはトランスアミナーゼ活性増加のみによるものかどうか疑わしい. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 80 7-8 1968 小脳の酵素組織化学―特にプルキンエ細胞のα-グリセロ燐酸脱水酵素と「固定の問題」をめぐつて― 665 676 EN Iwao Tokunaga (1) ダイコクネズミ及びヒト,ネコの小脳について,乳酸脱水素酵素(LDH),コハク酸脱水素酵素(SDH),α-グリセロ燐酸脱水素酵素(α-GPDH)活性を組織化学的に検索した. SDH,α-GPDHの検出にあたつてはintermediatorとしてmenadione (MD)を反応液に添加した. 新鮮凍結クリオスタット切片にて,LDHは顆粒層の“gloneruli cerebellosi”に相当して最も強く,分子層ではformazan顆粒はびまん性に形成されて反応は顆粒層よりやや弱くPurkinje細胞(P-細胞)は核を除いて細胞体に強度〜中等度の活性を示し,白質には活性はほとんど証明されなかつた. SDHは全体としてLDHの反応態度に似た像を示し,顆粒層に最も強く,ついでP-細胞層,分子層の順で活性が認められ,白質にはほとんど証明されなかつた. α-GPDH反応は,顆粒層,分子層においてはLDH, SDHとほぼ同じであるが,P-細胞層にはformazanの形成がほとんどなく,あたかも顆粒層と分子層の間に空隙がある様な像を示した.白質には活性はほとんど証明されなかつた,これらの所見は,これまでの報告にほぼ一致するものである. (2) 上記酵素活性の検出にあたり,新鮮凍結クリオスタット切片とともに,同一材料について組織塊をまず10%冷フォルマリンで固定した後に作製した凍結切片をあわせて使用した. SDH,α-GPDH, LDHともに反応は全般に減弱されるが,LDHの反応の減弱の度合は他の2者にくらべて少ないようであつた. ここで注目すべきことは,非固定切片のP-細胞層において,α-GPDHの活性がほとんど認められなかつたにもかかわらず固定切片のP-細胞には反応が認められ,しかもformazan形成は他の部よりもむしろ強度であつた点である.この事実は酵素組織化学における方法の選定や結果の判定にあたつては常に慎重な配慮が要求されることを示す現象であるとみなされ,この点に着目し特に固定の問題をめぐつて諸種の条件を実験的に設定してしらべた. (3) フォルマリン固定切片における反応には, i) 固定時間の長さによる著明な差異がみられない. ii) pHの変化による影響が少ない. iii) 特異的抑制剤による影響がみられない. iv) MDの存在が不可欠である. v) SH-基の関与がある. vi) Nitro-BTの組織への吸着性の増強が関連している. ということを認めた. このことからフォルマリン固定切片に見られる反応は,Nitro-BTの組織蛋白との結合性を前提とて,SH-groupとMDとが関与している非酵素的作用の結果であることを推論した. (4) 小脳組織でP-細胞が他の細胞要素と異なり,非固定切片においてα-GPDH活性をほとんど示さないこと,およびフォルマリン固定によりP-細胞に非酵素的なNitro-BT還元が著明にあらわれることは,P-細胞の細胞化学的特異性の一面を示すものであろう. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 3-4 1967 リンゴ酸脱水素酵素のアイソザイムに関する研究 第2編 ヒト血清及び発育過程におけるラット臓器のリンゴ酸脱水素酵素アイソザイムについて 271 280 EN Masao Takayasu Malic dehydrogenese (MDH) activities in human serum of various diseases were studied. Elevation of MDH activities were observed in myocardial infarction, acute hepatitis, and some malignant tumors. Characteristic MDH isozyme patterns were obtained from serum of patients with myocardial infarction and acute hepatitis showing marked elevation of MDH activities. During the tetrazolium procedure for staining of electrophoreticaly separated serum malic or lactic dehydrogenese isozymes on agargel, without substrate and co-enzyme, two peaks were noticed and named serum "non-specific factor". These peaks were considered a kind of non specific reactions which resulted from a reduction of the tetrazolium salt electrostaticaly adsorbed on Alb. 〜 α(1), Glb. by SH-groups of these serum proteins. These reactions were accelerated by the elavation of PH or the temperature, exposure during staining and the prolonged staining time, as well as the rise of concentrations of NTB, PMS and CN- in the staining medium. Considering of optimal conditions of enzyme reaction, the minimum use of NTB, PMS and Na CN, washing stained agargels with PH 4.5 acetic acid SoL, and complete shading during procedure could minimize the influence of this factor. Two MDH isozymes were demonstrated in agargel isozymograms of rat organs, one migrating towards the cathod and the other toward the anode. The cathodal fraction was considered mitochondrial MDH (m-MDH), and the anodal one cytoplasmic MDH (c-MDH), respectively. During development, m-MDH showed marked increase in heart muscle. In contrast to this, cMDH of liver and gastric mucosa increased in procedure of development. On the other hand the ratio of m-MDH and c-MDH in kidney remained constant through development. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 3-4 1967 リンゴ酸脱水素酵素のアイソザイムに関する研究 第1編 ヒト各種臓器のリンゴ酸脱水素酵素活性及びアイソザイムについて 259 270 EN Masao Takayasu Malic dehydrogenese (MDH) activities and isozymes of various human organs were measured. The assays of isozyme were carried out by means of agargel electrophoresis. The highest MDH activity was demonstrated in heart muscle. Liver, skeletal muscle, kidney and brain showed considerably high MDH activities. Six fractions were distinguished in many MDH isozymograms. In order of movility towards the cathod, each MDH isozyme was named MD(1), MD(2)…MD(6). Intracellular distribution of each MDH isozyme was investigated by means of cell fractionation, and MD(1)〜MD(5) were confirmed mitochondrial, MD(6) cytoplasmic, in origin. MDH isozyme-patterns of human organs were divided into 3 groups according to the ratio of mitochondrial MDH (m-MDH) and cytoplasmic MDH (c-MDH). Group �T (m-MDH>c-MDH): heart muscle, kidney, skeletal muscle, white blood cell, red blood cell and lung-tissues. Group �U (m-MDH≒c-MDH): liver, pancreas. Group �V (This group situates between Group �T and Group �U): brain, gastric mucosa, and spleen. Studies on inhibitory effect of p-chlor-mercuri-benzoate (PCMB) on each MDH isozyme and heat stability of each MDH isozyme demonstrated that m-MDH was not inhibited by PCMB and stable to a heat test at 50℃ for 30,' when 1-malate was used as substrate. It was also observed that m-MDH was activated by a high concentration of 1-malate. According to the standard deviation of MD(6) and MD(3) activites, c-MDH considered regulatory enzyme and m-MDH constitutive enzyme, respectively. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 82 5-6 1970 ヒトおよびネコの視床核電気刺激による皮質誘発反応に関する実験的研究 295 317 EN Shimpei Namba Cortical evoked responses after single or repetitive stimulations of the nucleus ventralis lateralis (VL), n. centrum medianum (CM) and n. ventralis anterior (VA) were observed in 61 cases of parkinsonism, 7 cases of intractable pain and a case of dystonia musculorum deformans from February, 1968 to December, 1969. Experimental studies of the thalamic stimulations with 15 cats were performed as well and the results were compared with that of the human. Evoked responses were analysed with averaging computer technique. Results were as followings. 1) Cortical evoked potentials after single stimulation of the VL nucleus of the human thalamus. a) Evoked potentials appeared on the ipsilateral centro-frontal regions with weaker stimulation in EEG records. However, the evoked potentials showed clear bilateral distribution except the temporal region, predominantly on both centro-frontal regions, with stronger stimulation electroencephalogaphically. b) The evoked potentials of the central site showed clear three negative waves within 200 msec after the stimulation, that is, the first negative wave (�T-N) with the peak time of about 10 msec, the second (positive-) negative wave (�U-PN) with the peak time of about 50 msec and the third negative wave (�V-N) with that of 80-100 msec with averaging computer records. The peak time of the �T-N wave and the negative phase of the �U-PN wave were nearly constant, but that of the �V-N wave considerably varied according to the background activities of EEG. The evoked potentials showed no significant discrepancy between right and left patterns, and moreover, they maintained a similar pattern when the stimulating voltages were changed. c) Bilateral evoked potentials were clearly seen with averaging computer technique even when evoked potentials were small and hardly identified on the contralateral scalp with EEG records. When the stimulating voltages were gradually decreased, the ipsilateral and contralateral evoked potentials disappeared approximately at the same time. d) The latency of the �T-N wave was measured to be 5-6 msec with averaging computer records, and the amplitude of the �T-N wave increased proportionately with the increase of stimulating voltages. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 84 11-12 1972 ヒト胆嚢粘膜及び胆嚢コレステローシスの電子顕微鏡的研究 575 593 EN Kimio Takahashi Human gall bladders specimens obtained soon after cholecystectomy from 50 patients were observed under electron microscopy. Four types of cells were distinguished by light and electron microscopy in the human gall bladder epithelium, i.e.,"Ordinary epithelial cell", "Clear cell", "Dark cell", and" Basal cell". The Clear cell have been subdivided at least two types by electron microscopy. One was degenerative type and another was immatured one. It was also observed that the mechanism of discharge of secretory granules was the" Merocrine mode". In the cholesterosis, it was assemed that cholesterol was absorbed into the epithelial cells from the bile and accumlated in their basal area, then discharged through their basis. It was taken by phagocytes which gradually transformed into the foamy cells. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 84 11-12 1972 低カタラーゼ血液症の簡易検出法に関する研究 第2編 ヒト血液凍結保存によるカタラーゼ活性度の変動について 395 396 EN No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 84 11-12 1972 低カタラーゼ血液症の簡易検出法に関する研究 第1編 ヒト血液保存によるカタラーゼ活性度の変動について 391 393 EN No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 84 9-10 1972 Thalamo-cortical Relationに関する電気生理学的研究-ヒト視床腹外側核刺激による皮質誘発反応の分析- 247 266 EN Toshihiko Miyamoto Electrophysiological studies on evoked cortical potentials following ventrolateral (VL) thalamic stimulation of man were performed with averaging computer technique for 41 patients of parkinsonism and other involuntary movement disorders during stereotaxic surgery for last 2 years. 1. Single stimulation of the VL nucleus demonstrated bilateral cortical activity with I-PN (first positive negative), �U-PN (second positive negative), �V-PN (third positive negative) and �W-PN (fourth positive negative) waves. �V-P wave was frequently superimposed on negative response between �U-N and �V-N wave, and �W-PN waves were frequently feeble in single stimulation of the VL nucleus. 2. Peak latencies of these waves were estimated 3.4±1.2 msec in �T-P, 10.6±1.8 msec in �T-N, 29±5 msec in �U-P, 55±6 msec in �U-N, 71±11 msec in �V-P, 90±11 msec in �V-N, 115±19 msec in �W-P and 160±24 msec in �W-N wave in ipsilateral central lead, and 4.0±1.5 msec in �T-P, 11.1±2.1 msec in �T-N, 31±4 msec in �U-P, 57±6 msec in �U-N, 74±13 msec in �V-P, 92±12 msec in �V-N, 118±24 msec in �W-P and 156±29 msec in �W-N wave in contralateral central lead. 3. The first deflection time of �T-P wave, which meant beginning of the evoked response, was 1.5-1.8 msec in stimulated side of central cortex and 2.2-2.9 msec in contralateral central lead, which were obtained in 5 cases precisely measured. 4. The impulse, which provoked �T-P N waves, was thought to be conducted from stimulated VL nucleus to the contralateral cortex directly via corpus callosum with 36-43m/sec of velocity. 5. Cortical evoked responses following suprathreshold low frequency stimulation (5-12Hz) of the VL nucleus showed invariably augmenting response and recruiting-like augmenting response. Augmenting response, which consisted of a train of growth in both. positive and negative components, was evoked when each stimulus was given on the descending phase from the peak of �V-N or augmented negativiy to the bottom of following positive wave of the preceding response. Analysis of augmentation suggested that synchronization of preceding evoked �W-P and present �U-P wave would be occured in augmented positivity and synchronization of preceding evoked �W-N and present �U-N or �V-N wave would be occured in augmented　negativity. Recruiting-like augmenting response was obtained when each stimulus was given on the ascending phase from the bottom of deep �W-P to the following negative wave of the preceding response. Recruiting-like augmentation was shown to be a similar response as augmenting response in the fundamental pattern of averaged evoked activity, although development of negativity and attenuation of positivity caused recruiting-like pattern. Component analysis of recruiting-like augmenting response revealed that predominant development of negativity was thought to be the result of the synchronization of preceding evoked �W-N and present �U-N or �V-N wave. And attenuation of �U-P wave was thought to be the result of the desynchronization of preceding �W-N and present �U-P wave. Responses following lower frequency stimulation with 4Hz or less were similar to responses following single stimulation. Subthreshold stimulation were thought to be difficult to induce any growth of negative cortical response. 6. It was clarified that cortical evoked response following stimulation of the VL nucleus was influenced by components and phases of the preceding cortical response, suggesting that human specific thalamic system would play a possible important role in modulation upon cortical electrical activity. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 86 7-8 1974 マウス腹腔内な挿入されたdiffusion chamber内でのヒトリンパ芽球様株細胞の培養 第2編 ヒトリンパ芽球様株細胞へのRauscher白血病ウィルスの感染 403 408 EN Shuko Yoshimoto Diffusion chambers containing EBV-bearing human lymphoblastoid cells were placed temporarily within the peritoneal cavity of BALB/c mice with Rauscher leukemia. Reestablished human leukocyte cultures became dually infected with C type and EBV particles and have continuously produced abundant C type particles. These virus particles were nonleukemogenic when inoculated into BALB/c mice but afforded some protection against challenge infection with spleen-derived leukemogenic RLV. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 86 7-8 1974 マウス腹腔内に挿入されたdiffusion chamber内でのヒトリンパ芽球様株細胞の培養 第1編 ヒトリンパ芽球様株細胞の形態学的変化 393 401 EN Shuko Yoshimoto A clonal lymphoblastoid cell line derived from lymph node of a patient with Hodgkin's diseasewas cultivated in diffusion chambers implanted in the abdominal cavity of mice and cellular morphologic changes were sequentially studied for up to 8 weeks. The majority of cells before intraperitoneal implantation were lymphoblasts with approximately 16% mature lymphocytes. However, after 3 weeks of in vivo cultivation in diffusion chambers, macrophage-like cells began to increase and occupied 80-90% of cells after 4 and 5 weeks. These macrophage-like cells, when tested for phagocytic activity after 4 weeks, exhibited bacterial phagocytosis. Labeling studies with (3)H-thymidine indicated that active DNA synthesis continued for up to 2 weeks of in vivo diffusion chamber cultivation, during which mitotic figures were also observed morphologically. Further, it was attempted to recultivate in vitro cells that were taken out of diffusion chambers after 2, 3, and 4 weeks of in vivo implantation. These 3 separate attempts led to the development of lymphoblastoid cells that were morphologically and cytogenetically similar to those prior to diffusion chamber implantation. From these findings, it is suggested that human lymphoid cells undergo morphologic alteration to macrophages under certain circumstances. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 11-12 1971 ヒト末梢血由来リンパ芽球様株細胞に関する研究 第2編 蛍光抗体法による研究 569 578 EN Harumi Hasegawa Three types of investigations by the immunofluorescence method were performed. Antibody to EB virus (EBV) in various sera and EBV antigen in the nine lymphoblastoid cell lines were tested by the indirect method and production of immunoglobulin in these cell lines was by the direct method. 1) It has been shown that the virus is widely disseminated among normal persons in Japan. 2) Newborn infants possess EBV antibody on delivery. This antibody is probably transmitted from mother to fetus via the placenta. 3) There is no difference in EBV antibody titers between normal individuals and donors from whom the lymphoblastoid cell lines were established. 4) EBV antigen appears in a small number of lymphocytes and macrophages within a few weeks after initiation of peripheral leukocyte cultures. 5) All the established cell lines persistly demonstrate a few percentage of EBV antigen-positive cells. 6 ) All the cell lines produce two or three classes of immunoglobulin but the proportion of the stained cells varies considerably during long-term culture. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 11-12 1971 ヒト末梢血由来リンパ芽球様株細胞に関する研究 第1編 リンパ芽球様株細胞の樹立 553 567 EN Harumi Hasegawa Nine new lymphoblastoid cell lines have been established from peripheral blood leukocytes of patients with infectious mononucleosis, subacute myelo-optico-neuropathy (SMON), acute myelogenous leukemia and chronic lymphatic leukemia. All the cell lines grow in suspension and mostly consist of lymphoblastoid cells regardless of the cell source and clinical status of the donors. A few percentage of Epstein-Barr virus (EBV) antigen-positive cells were detected by the immunofluorescence method in all the cell lines. These cell lines have maintained karyotypes of predominantly normal diploid modes. It is suggested that the origin of these cell lines may be certain lymphocytes in peripheral leukocytes. From the present study, a following hypothesis may be offered on the establishment of the lymphoblastoid cell lines. It is conceivable that EBV which is harbored in vivo in lymphocytes might be activated and replicate in the early stage of leukocyte culture. The virus would, thereafter, exert an antigenic stimulation on some of the lymphocytes and consequently they would undergo blastoid transformation, yielding self-sustaining cell lines. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 ヒトの胃の内分泌細胞の細胞学的研究 第�U編 病的状態の胃前庭部粘膜における内分泌細胞の組織化学的・電子顕微鏡的研究 247 258 EN Akira Nabeyama Normal and pathological antral mucosa (gastric ulcer, gastroduodenal ulcer, duodenal ulcer, gastric cancer, giant rugae, gastritis chronica and gastric polyp) were examined histochemically and electron microscopically. 1) It was clarified that the Ec-cells are observed only in the mucosa with intestinal metaplasia, and never in the normal mucosa. We tentatively classified the Ec-cells into two types; Ec-�T-cell and Ec-�U-cell. The specific granules in the Ec-�T-cells are polymorphous and contain a dense core enclosed by a limiting membrane which fits either tightly or loosely. The Ec-�U-cells have electron dense and polymorphous granules but do not contain a dense core in the limiting membrane. The ratio of Ec-�T-cells to Ec-�U-cells in the antral mucosa with intestinal metaplasia seems to be almost equal in gastric ulcer but smaller in gastric cancer. 2) Cell type �W-cell in the normal antral mucosa has three kinds of granules; electron dense granules (100-200 mμ in diameter), granules (about 400 mμ in diameter) which have cloud-like or bubble-like substances in the limiting membrane, and granules which are empty-looking in the limiting membrane. In gastric ulcer cases, the �W-type cells were apt to be granulated but there were no increse in the number. On the contrary, in atrophic gastritis, the �W-type cells had low electron dense granules which were empty-looking in the limiting membrane. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 ヒトの胃の内分泌細胞の細胞学的研究 第�T編 ヒトの正常胃前庭部粘膜にみられる内分泌細胞の電子顕微鏡的分類 235 245 EN Akira Nabeyama The endocrine cells in te normal antral mucosa of the human stomach were examined by the electron microscope. According to their fine structural characteristics, they were classified into the following five types. 1) Cell-type �U is triangular in shape. The granules are round and vary in diameter (300-600mμ) and in electron density. 2) Cell-type �V is triangular in form and has many grnules (130mμ in diameter) with high electron density. These granules are characterized by their uniform size and homogeneous appearance. 3) Cell-type �W contains a prominent endoplasmic reticulum in the supranuclear region. These kinds of granules are recognizable in the basal part: smaller ones (100-200mμ), which are dense, larger ones (400mμ) which are pale and another larger ones (400mμ) which are empty-looking. 4) Cell-type �Xis contact with the glandular lumen and contains numerous secretory granules with various densities. 5) Cell-type �Y is oval in shape and characterized by small granules (100-150mμ) with moderate density, localized in the basal region. This cell type seems to have some features of an immature endocrine cells. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 7-8 1973 大脳皮質電気活動の同期化における視床腹外側核の関与について-ヒトとネコにおける電気生理学的研究- 373 385 EN Hiroichi Beck Spindle-like afterdischarges evoked by stimulation of ventrolateral thalamic nucleus and spontaneous spindle waves were analized to investigate the electrophysiological mechanism of cortical synchronized activities in man as well as in cat. In man, three positive and three negative waves, namely �T-P, �T-N, �U-P, �U-N, �V-P and �V-N, were observed as cortical evoked responses after single stimulation of the ventrolateral thalamic nucleus. And �W-P and �W-N were observed after the stronger stimulation which could provoke augmenting response with the repetitive volley. Later components of evoked cortical responses were investigated in cat immobilized under local anesthasia. Recording electrole was located on the anterior sigmoid gyrus. As the intensity of the thalamic stimulation was increased, afterdischarges of the negative waves, which appeared with long latency (about 200 msec.), increased in number and developed into spindle-like pattern with notches on afterdischarges. The long latencied negative wave in cat and �W-N in man were considered to correspond to Lehtinen's "precursor of the evoked spindle activity". It has been already reported by Miyamoto that �W-N was supposed to play an important role in augmenting response in man. Close relationship was indicated between augmenting response and spindle-like afterdischarges. Futhermore, the similarities of the pattern between spindle-like afterdischarges following thalamic stimulation and spontaneous spindle waves in cat, and attenuation of ipsilateral spontaneous spindle waves after destruction of the ventrolateral thalamic nucleus in cat as well as in man were also observed. From these facts these cortical synchronized activities, namely AR, spindle-like afterdischarges and spontaneous spindle waves were considered to be brought by similar neuronal mechanism with some varieties of synchronization ascribed to arousal levels under influences of activities of the ventrolateral thalamic nucleus. On the other hand, spindle-like afterdischarges and spontaneous spindle waves in cat were analized for investigating their waxing and waning phenomena. Notches were observed on each waves. When the wave grew with maximal amplilude in a train of spindle, the notch of the wave became obscure. The phenomenon was considered that the notch was synchronized with the wave, forming maximal amplilude of the wave in a train of spindle. In other words, mechanism of this phenomenon was supposed to be explained as a beat of two rhythmic activities slightly different in frequency. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 5-6 1973 SV40ウイルス及びSV40誘発腫瘍細胞により感作されたリンパ球のSV40変異ヒト胎児細胞に対する細胞障碍作用 223 229 EN Kuniyuki Egusa Keiko Sato Takuzo Oda Sensitized lymphocytes have been demonstrated to inhibit tumor growth in vivo systems. In SV40-viral oncogenesis, some specified immunization with virns and with virus-induced tumors to the animals results in rejection of further transplantation of the tumor cells. To analyze these phenomena by our specially devised cytotoxicity test, the authors have studied cell mediated immunity for tumor specific transplantation antigen (TSTA) in SV40 viral oncogenesis. The results suggest that sensitized lymphocytes inhibit the growth of heterogenic SV40-transformed target cells as well as syngenic ones. And the lymphocytes from tumor-bearing animals have lower ability of the inhibition on the target cells, which may be explained that the lymphocytes in tumor bearing animals are under the condition of immunologically insufficient state. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 88 1-2 1976 ヒトの胸管壁の構造について 97 100 EN Kiichi Suwa In the human thoracic duct wall the direction and the ascending angle of the smooth muscle cells are not necessarily fixed according to the site, but as a rule the smooth muscle cells of the interior layer of the tunica media ascend longitudinally at about 80° with their origin at the internal elastic membrane and connect to the circular muscle of tunica media that ascends at angle of about 10° and terminates at the external elastic membrane. At the exterior side of tunica media it becomes often longitudinal and sometimes it forms the exterior layer. The smooth muscle cells which connect the middle layer and exterior layer from the interior layer run in a specific arc line. In the case where a pair of valves cover the thoracic wall, at the site between the two valves there can be observed no longitudinal muscle of the interior layer. Generally the internal elastic membrane is incomplete, and often it forms longitudinal networks, but there is sometimes the case where morphologically it forms a well arranged internal elastic membrane. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 87 11-12 1975 実験的アレルギー性肝炎の研究 第2編 同種肝抽出液感作リンパ球の移入による肝の組織変化 1023 1032 EN Hiroaki Hirata ヒトの肝炎の進展,慢性化の過程において,肝のグリソン鞘に浸潤している単核細胞がいかなる役割を果しているかを究明する一つの試みとして, complete Freund's adjuvant添加同種肝抗原でC57Blマウスを長期感作して実験的アレルギー性肝炎を作成し,感作C57Blマウスの脾およびリンパ節より感作リンパ球を分離し,レントゲン全身照射後の同系マウスに移入し,受動転嫁実験を実施した.その結果,対照群に比して,39例中15例に肝の組織変化を認めた.そのうち,早期すなわち移入後2〜3日目に発生した広範な凝固壊死を14例中3例に,やゝ遅れた時期すなわち移入後5〜8日目に発生したpiecemeal necrosisを主体とする変化を25例中2例に認め,それらの変化は感作リンパ球による肝細胞性壊死であることを強く示唆した.以上の肝の組織変化はヒトのウイルス性肝炎の組織所見に類似しており, B型肝炎発症における主要因の1つ,すなわちHB抗原感作リンパ球の果す意義にも関連して重要な実験成績であると考えた. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 87 11-12 1975 実験的アレルギー性肝炎の研究 第1編 AKRマウス肝抽出液によるC57Blマウス長期感作後の組織学的変化 1009 1021 EN Hiroaki Hirata ヒトのウイルス性肝炎の発症および慢性化のアレルギー性機序を解明するための一手段として, AKR肝抽出液を抗原としてC57Blに長期感作を施行し,肝グリソン氏鞘内に著明な円形細胞浸潤,piecemeal necrosis,肝細胞壊死,星細胞の反応を作成しえたが,リンパ沪胞の形成,肝グリソン氏鞘の結合織の増生は認められなかった.以上の組織変化は肝炎B抗原,すなわち,　hetero抗原とその抗体とのアレルギー機序によって生じると考えられるヒトのウイルス性肝炎の組織表現にかなり類似している.しかし,長期感作を中止し放置すると,組織変化は消褪した. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 87 9-10 1975 マウス白血病ウイルスのヒト培養白血球への感染に関する研究 第2編EBウイルスとC型ウイルスの重複持続感染系ヒトリンパ芽球様株細胞の異種移植 893 899 EN Takehiko Hayashi An Epstein-Barr virus (EBV) -positive human lymphoblastoid cell line chronically infected with Rauscher murine leukemia virus (RLV) was transplanted into antilymphocyte serum (ALS)- treated newborn hamsters. Four of 10 hamsters transplanted developed nonlethal regressive tumors which continued to shed C-type virus. Lymphoblastoid cell cultures reestablished from the heterotransplanted tumor cells were positive for both EBV capsid and murine gs-1 antigens. In contrast, all the hamsters transplanted with its parent cell line without RLV infection succumbed to lethal progressive tumors. The RLV-infected cells were shown to have cell surface antigen that was not found in the noninfected cells. It is postulated that new membrane antigen associated with C-type virus infection provoked more immunologic reaction even in ALS-treated hamsters, accounting for the reduced tumorigenicity of the RLV-infected human leukocytes. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 87 5-6 1975 マウス白血病ウィルスのヒト培養白血球への感染に関する研究 第1編 ヒトリンパ芽球様株細胞におけるEBウイルスとC型ウイルスの重複持続感染の成立 549 556 EN Takehiko Hayashi An EB virus-carrying lymphoblastoid cell line established from peripheral blood of a patient with SMON was super-infected in vitro with murine Rauscher leukemia virus. Since then, the cell line has been dually infected with both viruses for more than 1.5 years, as repeatedly demonstrated by the presence of herpes type virus particles and C type virus particles. The C type virus particles liberated from the human cultured leukocytes were no longer leukemogenic when inoculated into otherwise susceptible BALB/c mice, but this procedure rendered them considerably resistant to challenge infection with leukemogenic Rauscher leukemia virus that had been maintained by passage through BALB/c mice. The infectivity of the human cell-derived C type virus to other lymphoblastoid cell lines established from different patients and to primary BALB/c mouse embryo cells could not be demonstrated by the method employed. The implication of these findings is discussed in the light of possible viral etiology of human malignancy. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2009 フコース除去抗体のヒト末梢血中における抗体依存性細胞傷害（ADCC）活性誘導作用及びその機序に関する研究 EN Shigeru Iida No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 5-6 1978 ヒト胎児肝細胞の培養 第2報 629 639 EN Hiromichi Imai In the previous report, we dealt with the method and the culture of human embryonic liver cells, and its difficulties in establishing epithelial cell lines from normal human liver. HuL-4 cell strain was published as an epithelial cell strain in the previous paper. We examined the cell morphologically, histochemically, and the cellular localisation of albumin and AFP (α-fetoprotein) immunofluorescently. Chromosome of the cells and uptake of Indian ink into the cell were also undertaken. We also calculated the growth curve of the strain and analyzed the liver specific enzymes. The results are summarized as follows. 1). HuL-4 cell strain derived from human embryonic liver disclosed dominant epithelial cell growth than fibroblastic cells, and it continued to cultivate up to 24 passages in 758 days. 2). Direct immunofluorescence method for albumin and AFP failed to show any positive stain. 3). PAS staining of the cell revealed weak positive, and it was digested by diastase. PAP silver staining was negative, and the uptake of Indian ink into the cells was negative for up to 12 hours. 4). Chromosome analysis of the cell was normal (46 numbers, diploid) and no marker chromosomes were detected. 5). Hul-4 cell strain was epithelial morphologically, but it contained no liver specific enzymes. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2009 Studies of Starfish Protein Kinase C Isoforms and Cdc25 Protein Phosphatase EN Yasuo Miyake No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2009 Bone Repair by Transplantation of hTERT-Immortalized Human Mesenchymal Stem Cells in Mice EN Hiroyuki Nakahara No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 89 11-12 1977 乳癌の酵素活性に関する研究 第2編 ヒト乳腺組織の酵素活性ならびにヒト乳癌組織の酵素活性に及ぼすホルモンの影響 1597 1607 EN Makoto Inoue Glycolytic enzyme activities of human breast cancer was studied on age, menopausal status, T, N, Stage and pathology in benign and malignant tumor. Influences on incubation with additional hormones also were studied. Mastopathy, benign tumor and breast cancer showed varied enzymic activity. High enzymic activity was observed in breast cancer tissue, but the enzymic activity was not paralleled with tumor size. Enzymic activity changed by incubation with additional hormones. Pre menopausal state, T(1), negative lymph node metastasis, Stage I tissues were elevated enzymic activity. Post menopausal state, T(3), positive lymph node metastasis, and Stage III tissues were decreased enzymic activity. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 3-4 1978 ヒトの慢性胃炎胃粘膜の電子顕微鏡的走査型電子顕微鏡的観察 265 294 EN Tamotsu Imajo Thirty-six cases of human atrophic chronic gastritis were observed by transmission and scanning electron microscope. Generally, in the surface and neck mucous cells, vacuole formations and swelling of mitochondria were occasionally observed. Although, increase of young parietal cells were frequently noted, ultrastructural changes were hardly seen in the cytoplasm and the nucleus of both the parietal cells and the chief cells. Intestinal metaplastic mucosa was found in almost all cases of antral mucosa and in half cases of corporal mucosa of chronic gastritis. In the SEM observation, the gastric mucosa and the intestinal metaplastic mucosa were clearly bordered. In TEM observations, all types of normal small intestinal cells were found in the intestinal metaplastic mucosa, and there were no ultrastructural differences between normal intestinal cells and intestinal metaplastic mucosa cells. It was interesting that enterochromaffin cells, which were scarce in normal gastric mucosa and numerous in small intestine, were frequently observed in intestinal metaplastic mucosa. In the three cases of chronic gastritis, the peculiar type cells which had both endocrine cells granules and mucous granules were found and their origins were discussed. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 89 9-10 1977 ヒト消化管からのHorseradish Peroxidaseの吸収の細胞化学的研究 1329 1357 EN Teruo Matsumoto Using horseradish peroxidase (HRP) which serves as its own tracers, the absorption of intact macromolecules through the human gastrointestinal tracts was observed. In healthy volunteers, HRP was poured into the stomach, the duodenum, and the ileum through the gastric, duodenal and colonofiberscopes at several days interval. Twenty minutes after the injection, small samples of mucosa were removed. The tissues thus obtained were treated in the manner of Karnovsky and embedded in Epon and observed under an electron microscope. The absorption of HRP through the human small intestine and the intestinal metaplastic epithelia of the stomach was clearly demonstrated. Moreover, in intestinalized epithelium of the stomach, HRP was absorbed and transferred into the capillary lumen. These results indicated the posibility of absorption of macromolecules through the human gastrointestinal tract, which may be a cause of food-allergy and food poisoning. At the same time, it showed the effectiveness of oral administration of some enzyme-containing medicines. The absorption through the intestinal metaplastic epithelium was considered to be especially significant, because eaten proteins are promptly absorbed before digestion occurred. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 89 9-10 1977 ラットおよびヒトにおけるcaveolated cellの細胞学的研究 1221 1243 EN Takehiko Okuda Firstly, the ultrastructure of the caveolated cells on the rat bile duct were examined by a transmission and a scanning electron microscope. Next, the author seeked whether caveolated cells were present or not in the human digestive organs. Lastly, functional study of the caveolated cells on the rat bile duct were investigated using horseradish peroxidase and pilocarpine. Then the facts mentioned below were clarified. 1) Caveolated cells in the rat bile duct were found most densely at the divelticulum-like recesses but on the luminal surface except this recesses caveolated cells were found partly densely and partly sparsely. 2) On the rat, the straight filaments of the caveolated cells of the bile duct were less developed than that of the digestive tract, but circular filaments were more developed than that of the digestive tract. 3) The apical vesicles of the caveolated cells were revealed to have no communication to outer lumen of these cells using lanthanum as a tracer. 4) The caveolated cell-like cells were found in the mucosa of a human colon. But these cells could not be identified clearly with caveolated cells. Because the vesicles were scanty and small in size and the inner substance was relatively electron opaque. 5) After intraluminal injection of peroxidase into ligated segments of bile duct, the marker was found within the apical vesicles. But it was not uncertain whether pre-existing apical vesicles had taken up the marker or vesicles containing the marker were formed newly by pinocytosis. 6) After intraperitoneal injection of pilocarpine, the supranuclear vesicles began to expand and the vesicles with granules moved toward the free surface suggesting eruption or diacrine secretion. And frequently apocrine secretion was also observed. In conclusion, the author supposes that caveolated cell may have both absorptive and secretory functions and this type cells may present in human digestive tract. No potential conflict of interest relevant to this article was reported.