Aggregate-forming ability of liver cell lines derived from DAB-fed rats in rotation culture

For the purpose of elucidating more exact relationship between the process of carcinogenesis and aggregate-forming ability, we performed rotation cultures of a series of five liver cell lines derived from rats fed DAB for various period of d:l ys. As a result we found a tendency of the cells obtained from rats fed DAB for a longer period to form larger aggregates. The differences of the aggregate.forming ability among these cell lines were demonstrated well within one day, and more prominently after three days in rotaion culture. Histologically, the aggregates of all cell lines were composed of cuboidal epithelial cells, especially in some cell lines showing gland-like structures.