Identification of mouse H-2 antigens by mixed lymphocyte culture in the presence of PHA. 3. Blastformation of mouse lymphocytes according to the difference in non- H-2 antigens

It has been demonstrated that by the mixed cultures in the presence of PHA the combination of those cells whose H-2 antigens differ from each other shows a higher rate and more significant difference of blastformation than in the combination where non-H-2 antigens differ (Table 1). The blastformation observable in the combinations where non-H-2 histocompatibility antigens or sex.linked antigens are weaker, is not, so marked as the difference seen of the blastformation in the case with H-2 isoantigens. This in vitro lymphocyte stimulation test can be applied to the histocompatibility test in the combinations of strong H-2 antigens.