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ID 18124
Eprint ID
18124
FullText URL
80_625.pdf 291 KB
Title Alternative
Studies on the Measurement of Human Blood Catalase Activity and the Screening Method of Hypocatalasemia by Spectrophotometry Part 3. Evaluation of UV method supplemented with basic experiments on the measurement of human blood catalase activity
Author
Nakagawa, Yoshito
Abstract
The present communication deals with the principle of measuring and calculating the catalase activity of human blood by UV method. Under the conditions previously described, it has been clarified that the reaction of catalase-hydrogen peroxide practically follows the first order kinetics within 30-120 seconds. The velocity constant, k, seems to have no concern with the substrate concentration within the range of 0.005-0.025 mole. Catalase values, Kcat, measured under room temperature, are about 10% lower than those taken at 37℃. It has been found that measurements of the catalase activity have to be taken within 5 minutes after hemolysis
Published Date
1968-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume80
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
625
End Page
630
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/80/5-6/80_5-6_625/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
Japanese
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma