start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=
article-no=
start-page=e5
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2026
dt-pub=2026
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of sagging correction calibration error on radiation therapy equipment using image analysis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Purpose: This study investigates the effect of sagging correction errors on image quality and geometric coordinate accuracy.<br>
Methods: This study utilised the Elekta radiotherapy system, ball bearing (BB), Catphan phantom and MultiMet-WL phantom. Ten distinct flex maps (FMs) were acquired by positioning the BB at the accuracy isocentre and introducing shifts of 0.2, 0.4 and 0.6 mm in the left, table and up directions, respectively. Cone-beam computed tomography images of the Catphan phantom were acquired using 10 FMs. The images were analysed for modulation transfer function (MTF) values and geometric coordinates. Additionally, the Winston–Lutz (W-L) test was conducted under reference couch positions and with a 0.3 mm couch shift.<br>
Results: For the Catphan phantom analysis, the standard deviations of MTF10% across FMs were 0.19. The centre-of-gravity coordinates of the insert exhibited shifts of approximately 0.2, 0.4 and 0.6 mm when comparing reference images to those acquired with the shifted FMs. The results of the W-L test with a 0.3 mm couch shift showed radiation isocentre deviations exceeding 1 mm compared to the reference couch positions.<br>
Conclusions: Minor sagging correction calibration errors did not remarkably impact image quality; however, they altered the geometric coordinates of the image isocentre. These calibration errors decreased the accuracy of off-isocentre positioning.
en-copyright=
kn-copyright=

en-aut-name=FujiiYasushi
en-aut-sei=Fujii
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NakayamaTakahiro
en-aut-sei=Nakayama
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OshitaJunki
en-aut-sei=Oshita
en-aut-mei=Junki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TsunodaAyaka
en-aut-sei=Tsunoda
en-aut-mei=Ayaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SaekiYusuke
en-aut-sei=Saeki
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanabeYoshinori
en-aut-sei=Tanabe
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Radiology, Chugoku Central Hospital of the Mutual Aid Association of Public School Teachers
kn-affil=

affil-num=2
en-affil=Department of Radiology, Chugoku Central Hospital of the Mutual Aid Association of Public School Teachers
kn-affil=

affil-num=3
en-affil=Department of Radiology, Chugoku Central Hospital of the Mutual Aid Association of Public School Teachers
kn-affil=

affil-num=4
en-affil=Department of Radiology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Radiological Technology, Kawasaki Medical School Hospital
kn-affil=

affil-num=6
en-affil= Faculty of Medicine, Graduate School of Health Sciences, Okayama University
kn-affil=
en-keyword=flex map
kn-keyword=flex map
en-keyword=sagging
kn-keyword=sagging
en-keyword=Winston–Lutz test
kn-keyword=Winston–Lutz test
END

start-ver=1.4
cd-journal=joma
no-vol=237
cd-vols=
no-issue=
article-no=
start-page=113001
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202512
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Impact of different X-ray tube positions on actual dose measurements during CT examinations -An effect of patient physique-
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Dose management of patients is very important during X-ray Computed Tomography (CT) examinations, but because the patient's surface dose is inhomogeneous, it is difficult to measure the most probable value using a small passive-type dosimeter, lent to the patient. To solve this problem, our research group developed a precise dose analysis procedure in which a systematic uncertainty related to the X-ray incident direction (θin) is reduced. θin information was analyzed from CT images. However, the applicability of our procedure to actual patients with various physiques has not been examined. This study aims to propose a dose analysis procedure that can be applied to patients with various physiques, and to show its impact on dose measurement. Clinical data of 198 patients with Body Mass Index (BMI) values between 15 and 40 kg/m2 (mean value: 23.1 ± 3.8 kg/m2) who underwent chest CT scans were analyzed after dividing them into three groups based on BMI values. The absorbed dose was measured with a small-type Optically Stimulated Luminescence (OSL) dosimeter. To derive correction factors related to θin, the dependence of the actually-measured dose values of various patients on θin was analyzed. The correction coefficients were determined independently for the three groups classified by BMI values. By correcting the effect of θin, the systematic uncertainty element could be reduced, resulting in 30 % reduction of the uncertainty. Furthermore, it was found that our analysis procedure makes it possible to visualize outliers. In comparison with the expected dose values based on Computed Tomography Dose Index (CTDI) values, most of the data fell within the range of ±1.34 mGy (=1σ). However, 7 % of the data showed large deviations larger than 2σ. In conclusion, our research group has developed a procedure for measuring patient surface doses that can be applied to patients having various physiques, in which the effects of X-ray incident direction were accurately corrected. The procedure could be one solution to the problems with actual dose measurements during CT examinations, and will be useful for dose management based on the small-type dosimeter.
en-copyright=
kn-copyright=

en-aut-name=HayashiHiroaki
en-aut-sei=Hayashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MaedaTatsuya
en-aut-sei=Maeda
en-aut-mei=Tatsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakegamiKazuki
en-aut-sei=Takegami
en-aut-mei=Kazuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=GotoSota
en-aut-sei=Goto
en-aut-mei=Sota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=AsaharaTakashi
en-aut-sei=Asahara
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KimotoNatsumi
en-aut-sei=Kimoto
en-aut-mei=Natsumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NishigamiRina
en-aut-sei=Nishigami
en-aut-mei=Rina
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KobayashiDaiki
en-aut-sei=Kobayashi
en-aut-mei=Daiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KanazawaYuki
en-aut-sei=Kanazawa
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=YamashitaKazuta
en-aut-sei=Yamashita
en-aut-mei=Kazuta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KonishiTakeshi
en-aut-sei=Konishi
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=MakiMotochika
en-aut-sei=Maki
en-aut-mei=Motochika
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

affil-num=1
en-affil=College of Transdisciplinary Sciences for Innovation, Kanazawa University
kn-affil=

affil-num=2
en-affil=Graduate School of Medical Sciences, Kanazawa University
kn-affil=

affil-num=3
en-affil=Department of Radiological Technology, Yamaguchi University Hospital
kn-affil=

affil-num=4
en-affil=Faculty of Health Sciences, Kobe Tokiwa University
kn-affil=

affil-num=5
en-affil=Department of Radiological Technology, Faculty of Health Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Radiological Science, Faculty of Health Sciences, Junshin Gakuen University
kn-affil=

affil-num=7
en-affil=Graduate School of Medical Sciences, Kanazawa University
kn-affil=

affil-num=8
en-affil=Graduate School of Medical Sciences, Kanazawa University
kn-affil=

affil-num=9
en-affil=Faculty of Life Science, Kumamoto University
kn-affil=

affil-num=10
en-affil=Department of Orthopedics, School of Medicine, Tokushima University
kn-affil=

affil-num=11
en-affil=MEDITEC JAPAN Co., Ltd.
kn-affil=

affil-num=12
en-affil=MEDITEC JAPAN Co., Ltd.
kn-affil=
en-keyword=Patient dosimetry
kn-keyword=Patient dosimetry
en-keyword=Medical diagnosis
kn-keyword=Medical diagnosis
en-keyword=OSL dosimeter
kn-keyword=OSL dosimeter
en-keyword=X-ray CT
kn-keyword=X-ray CT
en-keyword=Passive type radiation dosimeter
kn-keyword=Passive type radiation dosimeter
en-keyword=BMI
kn-keyword=BMI
END

start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=2
article-no=
start-page=25-00212
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=2025
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=DNS analysis on the correlation between local burning velocity and flame displacement speed of turbulent premixed flames
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The local burning velocity and flame displacement speed are the major properties of premixed flames. The local burning velocity, which is the instantaneous quantity based on the local consumption rate of the unburnt mixture, is considered to be the most appropriate burning velocity in terms of the definition. The local burning velocity can be evaluated theoretically and numerically; however, it is almost impossible to obtain it experimentally using the current technology of measurement. The flame displacement speed can be evaluated more easily than the local burning velocity and compared with the flame displacement speed obtained from experiments. However, the local burning velocity and flame displacement speed have been discussed separately in turbulent premixed flames. In this study, to clarify the relation between the local burning velocity and the flame displacement speed, numerical analyses were performed using the DNS database of statistically steady and fully developed turbulent premixed flames with different density ratios of the unburnt mixture to the burnt product and with different Lewis numbers. It was found that for different density ratios, the local burning velocity was little sensitive to the flame displacement speed in any case under the unity Lewis number. This means the correlation between the local burning velocity and the flame displacement speed is little affected by the dilation of a flame. For different Lewis numbers, the correlation between the local burning velocity and the flame displacement speed was negative in Le = 0.8, and positive in Le = 1.2. This can be explained by the effect of the Lewis number on the local burning velocity, and the flame displacement speed was little affected by the Lewis number in the correlation between the local burning velocity and the flame displacement speed.
en-copyright=
kn-copyright=

en-aut-name=TSUBOIKazuya
en-aut-sei=TSUBOI
en-aut-mei=Kazuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Faculty of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Direct Numerical Simulation (DNS)
kn-keyword=Direct Numerical Simulation (DNS)
en-keyword=Turbulent premixed flame
kn-keyword=Turbulent premixed flame
en-keyword=Local burning velocity
kn-keyword=Local burning velocity
en-keyword=Flame displacement speed
kn-keyword=Flame displacement speed
en-keyword=Density ratio
kn-keyword=Density ratio
en-keyword=Dilation
kn-keyword=Dilation
en-keyword=Lewis number
kn-keyword=Lewis number
END

start-ver=1.4
cd-journal=joma
no-vol=15
cd-vols=
no-issue=2
article-no=
start-page=191
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250219
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Palladium-Catalyzed Decarbonylative Nucleophilic Halogenation of Acid Anhydrides
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In this study, we developed a palladium-catalyzed decarbonylative nucleophilic halogenation reaction using inexpensive and readily available acid anhydrides as substrates. This approach effectively circumvents the instability of acyl chlorides and the low reactivity of acyl fluorides. The Pd/Xantphos catalyst system exhibited excellent compatibility with the thermodynamically and kinetically challenging reductive elimination of C–X bonds (X = I, Br, and Cl) from Pd(II) intermediates. Notably, for electron-donating substrates, adopting an open system significantly improved the reaction efficiency. The positive effect of the open system may be due to the reversible nature of CO insertion and deinsertion, which helps direct the reaction toward the desired pathway by allowing the generated CO to exit the reaction system. Mechanistic studies suggest that the reaction proceeds through a highly reactive acyl halide intermediate, followed by a unimolecular fragment coupling (UFC) pathway via decarbonylation or an alternative pathway involving the formation of an activated anionic palladate complex in the presence of lithium halide.
en-copyright=
kn-copyright=

en-aut-name=TianTian
en-aut-sei=Tian
en-aut-mei=Tian
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UeiShuhei
en-aut-sei=Uei
en-aut-mei=Shuhei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YanWeidan
en-aut-sei=Yan
en-aut-mei=Weidan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NishiharaYasushi
en-aut-sei=Nishihara
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Chemistry, Faculty of Science, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=4
en-affil=Research Institute for Interdisciplinary Science (RIIS), Okayama University
kn-affil=
en-keyword=reductive elimination of C–X bond
kn-keyword=reductive elimination of C–X bond
en-keyword=nucleophilic halogenation
kn-keyword=nucleophilic halogenation
en-keyword=unimolecular fragment coupling (UFC)
kn-keyword=unimolecular fragment coupling (UFC)
en-keyword=acid anhydrides
kn-keyword=acid anhydrides
en-keyword=aryl halides
kn-keyword=aryl halides
END

start-ver=1.4
cd-journal=joma
no-vol=82
cd-vols=
no-issue=2
article-no=
start-page=26-1566
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2026
dt-pub=2026
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=放射線治療装置の回転座標系誤差が軸外targetの照射精度に及ぼす影響とTG142のトレランスの評価
en-subtitle=
kn-subtitle=
en-abstract=Purpose: The aim of this study was to quantitatively evaluate the impact of gantry, collimator, and couch rotational errors in a linear accelerator on the irradiation accuracy of off-isocenter targets, and to assess the validity of the rotational error tolerance (±1.0°) specified in American Association of Physicists in Medicine TG142. Methods: Using an Elekta linear accelerator (Elekta, Stockholm, Sweden) and the MultiMet-WL QA phantom (Sun Nuclear, Melbourne, FL, USA), an off-isocenter Winston–Lutz test was performed on six targets. In addition to baseline measurements, six conditions were evaluated by intentionally introducing rotational errors of +0.5° and +1.0° in the collimator, gantry, and couch. The vector distance (S value) between the field center and the target center, as well as positional deviations in each direction (gantry-target: GT, left-right: LR, anterior-posterior: AP), were analyzed. Results: Targets located farther from the isocenter exhibited more significant positional deviations. The collimator rotation had the greatest impact; at 7 cm from the isocenter, even a 0.5° error resulted in a maximum S value of 1.24 mm. Couch rotation had the next largest effect, while gantry rotation had relatively smaller effects, likely because most targets were located near the gantry’s rotational axis. The rotational errors mainly caused geometric deviations with direction-dependent positional shifts. Conclusion: The effects of the collimator and couch were substantial, with positional deviations exceeding 1 mm even for a 0.5° rotation error. The influence of the gantry was relatively small and dependent on the target configuration. For irradiation of off-axis targets, the TG142 tolerance of ±1.0° should be regarded as a minimum standard that must be strictly observed regardless of the type of linear accelerator. However, depending on the target arrangement, clinically adequate margins may not be ensured. These findings suggest the necessity of applying stricter criteria according to target configuration and emphasize the importance of regular quality assurance.
kn-abstract=【目的】放射線治療装置の回転座標系の誤差が軸外targetの照射精度に及ぼす影響を定量的に評価し，TG142における回転座標系誤差（±1.0°）のトレランスの妥当性を検討する．【方法】Elekta社製放射線治療装置（Elekta, Stockholm, Sweden）とMultiMet-WL QAファントム（Sun Nuclear, Melbourne, FL, USA）を用いて，6個のtargetに対してoff isocenterのWinston–Lutz test（WL test）を実施した．Baselineの測定に加え，意図的にcollimator，gantry，couchに+0.5°, +1.0°回転誤差を加えた6条件で測定を行い，照射野中心とtarget中心のベクトル距離（S値）および各方向（gantry-target: GT, left-right: LR, anterior-posterior: AP）の位置ずれを解析した．【結果】Isocenterからの距離が大きいtargetほど位置ずれが顕著であった．特にcollimator回転誤差の影響が最も大きく，isocenterから7 cm離れたtargetでは0.5°の回転誤差でもS値が最大1.24 mmに達した．次に影響が大きかったのはcouch回転であり，gantry回転はtargetの配置が回転軸に近いものが多く相対的に影響が少なかった．回転座標系の誤差は幾何学的誤差の影響が強く，位置ずれに方向依存性があった．【結語】Collimatorやcouchの影響が大きく，0.5°の誤差でも1 mm以上の位置ずれが生じることがあった．Gantryの影響はtargetの配置依存があり，相対的に小さかった．軸外targetの照射において，TG142の±1.0°のトレランスは放射線治療装置の種類にかかわらず最低限遵守するべき基準であり，targetの配置次第では臨床的に十分なマージンを保証できない可能性が示された．Target配置に応じたより厳格な基準と定期的quality assurance（QA）の重要性が示唆された．
en-copyright=
kn-copyright=

en-aut-name=NakayamaTakahiro
en-aut-sei=Nakayama
en-aut-mei=Takahiro
kn-aut-name=中山貴裕
kn-aut-sei=中山
kn-aut-mei=貴裕
aut-affil-num=1
ORCID=

en-aut-name=TanabeYoshinori
en-aut-sei=Tanabe
en-aut-mei=Yoshinori
kn-aut-name=田辺悦章
kn-aut-sei=田辺
kn-aut-mei=悦章
aut-affil-num=2
ORCID=

en-aut-name=FujiiYasushi
en-aut-sei=Fujii
en-aut-mei=Yasushi
kn-aut-name=藤井康志
kn-aut-sei=藤井
kn-aut-mei=康志
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Department of Radiology, Public Mutual Aid Association Chugoku Central Hospital
kn-affil=公立学校共済組合中国中央病院放射線科

affil-num=2
en-affil=Department of Radiological Technology, Graduate School of Health Sciences, Okayama University
kn-affil=岡山大学学術研究院保健学域放射線技術科学専攻

affil-num=3
en-affil=Department of Radiology, Public Mutual Aid Association Chugoku Central Hospital
kn-affil=公立学校共済組合中国中央病院放射線科
en-keyword=off-isocenter Winston–Lutz test
kn-keyword=off-isocenter Winston–Lutz test
en-keyword=rotation error
kn-keyword=rotation error
en-keyword=off-axis targets
kn-keyword=off-axis targets
en-keyword=Elekta
kn-keyword=Elekta
en-keyword=TG142
kn-keyword=TG142
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=1
article-no=
start-page=20
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20251021
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Natural Effects and Separable Effects: Insights into Mediation Analysis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Purpose of Review We compare natural effects and separable effects under nonparametric structural equation models with independent errors, highlighting their similarities and differences. By examining their required properties and sufficient conditions for identification, we aim to provide deeper insights into mediation analysis.<br>
Recent Findings If certain assumptions about confounding, positivity, and consistency are met, we can identify natural direct and indirect effects under nonparametric structural equation models with independent errors. However, these effects have been criticized because they rely on a specific cross-world quantity, and the so-called cross-world independence assumption cannot be empirically verified. Furthermore, interventions on the mediator may sometimes be challenging to even conceive. As an alternative approach, separable effects have recently been proposed and applied in mediation analysis, often under finest fully randomized causally interpretable structured tree graph models. These effects are defined without relying on any cross-world quantities and are claimed to be identifiable under assumptions that are testable in principle, thereby addressing some of the challenges associated with natural direct and indirect effects.<br>
Summary To conduct meaningful mediation analysis, it is crucial to clearly define the research question of interest, and the choice of methods should align with the nature of the question and the assumptions researchers are willing to make. Examining the underlying philosophical perspectives on causation and manipulation can provide valuable insights.
en-copyright=
kn-copyright=

en-aut-name=SuzukiEtsuji
en-aut-sei=Suzuki
en-aut-mei=Etsuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShinozakiTomohiro
en-aut-sei=Shinozaki
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamamotoEiji
en-aut-sei=Yamamoto
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Department of Epidemiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Interfaculty Initiative in Information Studies, the University of Tokyo
kn-affil=

affil-num=3
en-affil=Okayama University of Science
kn-affil=
en-keyword=Causality
kn-keyword=Causality
en-keyword=Counterfactuals
kn-keyword=Counterfactuals
en-keyword=Cross-world independence assumption
kn-keyword=Cross-world independence assumption
en-keyword=Directed acyclic graphs
kn-keyword=Directed acyclic graphs
en-keyword=Mediation analysis
kn-keyword=Mediation analysis
en-keyword=Nonparametric structural equation models with independent errors
kn-keyword=Nonparametric structural equation models with independent errors
END

start-ver=1.4
cd-journal=joma
no-vol=79
cd-vols=
no-issue=5
article-no=
start-page=359
end-page=368
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202510
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Advantages of Single-Position Surgery over Posterior Fusion for Single-Level Degenerative Lumbar Diseases
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Single-position surgery with lateral lumbar interbody fusion (LLIF) and percutaneous pedicle screws (PPSs) is gaining attention for its reduced invasiveness. We developed SPAPS, a technique allowing two surgeons to perform anterior LLIF and posterior PPS insertion simultaneously in a single lateral decubitus position. This retrospective study compared SPAPS (SPAPS-LLIF, Group SL) and minimally invasive posterior/transforaminal lumbar interbody fusion (MIS-PLIF/TLIF, Group PT) in patients treated between 2016 and 2019 with a two-year follow-up. Operative time, estimated blood loss (EBL), length of hospital stay (LOS), JOABPEQ and VAS scores, segmental lordotic angle, lumbar lordotic angle, segmental Cobb’s angle, PPS misplacement, PPS loosening, fusion status, and muscle cross-sectional areas were compared. Fifty-two patients were analyzed (Group SL: 25; Group PT: 27). SPAPS significantly reduced operative time (118.0 vs. 165.3 min, p <0.01) and estimated blood loss (8.6 vs. 164.1 mL, p<0.01). While clinical outcomes and hospital stay were comparable, Group SL had significantly lower PPS loosening (0% vs. 13%, p<0.01) and non-union rates (0% vs. 22.2%, p=0.02). Multifidus muscle atrophy was also less in Group SL (−14.3 vs. −121.5 mm2, p<0.01). SPAPS demonstrated advantages in reducing surgical invasiveness without compromising clinical efficacy, offering a promising alternative to conventional posterior fusion surgery.
en-copyright=
kn-copyright=

en-aut-name=HiroseTomohiko
en-aut-sei=Hirose
en-aut-mei=Tomohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IkumaHisanori
en-aut-sei=Ikuma
en-aut-mei=Hisanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OtsukaKazutoshi
en-aut-sei=Otsuka
en-aut-mei=Kazutoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KawasakiKeisuke
en-aut-sei=Kawasaki
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Orthopedic Surgery, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=2
en-affil=Department of Orthopedic Surgery, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=3
en-affil=Otsuka Orthopedic Clinic
kn-affil=

affil-num=4
en-affil=Department of Orthopedic Surgery, Kagawa Prefectural Central Hospital
kn-affil=
en-keyword=single-position surgery
kn-keyword=single-position surgery
en-keyword=simultaneous
kn-keyword=simultaneous
en-keyword=lateral decubitus positioning
kn-keyword=lateral decubitus positioning
en-keyword=lateral lumbar interbody fusion
kn-keyword=lateral lumbar interbody fusion
en-keyword=posterior lumbar interbody fusion
kn-keyword=posterior lumbar interbody fusion
END

start-ver=1.4
cd-journal=joma
no-vol=79
cd-vols=
no-issue=4
article-no=
start-page=269
end-page=278
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202508
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Femoral and Global Femoral Offset, but not Anteroposterior Offset, to Improve Postoperative Outcomes Following Total Hip Arthroplasty: Considerations Independent of the Contralateral Side
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The global femoral offset (the sum of the acetabular and femoral offsets) influences outcomes after total hip arthroplasty (THA). The optimal offset using plain radiographs has been reported, but internal and external rotations of the hip affect the offset value, producing unclear results when the nonsurgical side is not intact. We investigated the relationship between a functional hip score, i.e., the Harris Hip Score (HHS) and its effect on the post-THA anteroposterior and lateral offsets, and we sought to identify the optimal offset value. The cases of 158 patients with hemilateral hip osteoarthritis who underwent THA at a single center were retrospectively analyzed in this cross-sectional study. Three-dimensional pelvic and femoral models generated from computed tomography were used to examine several parameters, and the results revealed a significant binomial correlation among the modified HHS and femoral and global femoral offsets, with maximum values of 21.3 mm and 40 mm/100 cm body height, respectively. Pelvic and femoral parameters were measured and evaluated via alignment with a specific coordinate system. Our findings indicate that preoperative planning using these parameters may improve postoperative hip function, even when the nonoperative side is unsuitable for use as a reference, as in bilateral hip osteoarthritis cases.
en-copyright=
kn-copyright=

en-aut-name=ImaiNorio
en-aut-sei=Imai
en-aut-mei=Norio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HiranoYuki
en-aut-sei=Hirano
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HommaDaisuke
en-aut-sei=Homma
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=EndoYuki
en-aut-sei=Endo
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HorigomeYoji
en-aut-sei=Horigome
en-aut-mei=Yoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SuzukiHayato
en-aut-sei=Suzuki
en-aut-mei=Hayato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KawashimaHiroyuki
en-aut-sei=Kawashima
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Division of Comprehensive Musculoskeletal Medicine, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=2
en-affil=Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=3
en-affil=Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=4
en-affil=Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=5
en-affil=Division of Comprehensive Musculoskeletal Medicine, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=6
en-affil=Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=7
en-affil=Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=
en-keyword=total hip arthroplasty
kn-keyword=total hip arthroplasty
en-keyword=global femoral offset
kn-keyword=global femoral offset
en-keyword=postoperative outcome
kn-keyword=postoperative outcome
en-keyword=three-dimensional analysis
kn-keyword=three-dimensional analysis
en-keyword=anteroposterior offset
kn-keyword=anteroposterior offset
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250220
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Supplement-induced acute kidney injury reproduced in kidney organoids
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Introduction: Acute kidney injury associated with the consumption of Beni-koji CholesteHelp supplements, which contain red yeast rice (Beni-Koji), has become a significant public health concern in Japan. While renal biopsy findings from several case reports have suggested tubular damage, no definitive causal relationship has been established, and the underlying mechanisms of kidney injury remain poorly understood. The complexity of identifying toxic substances in supplements containing various bioactive compounds makes conventional investigative approaches both time-consuming and challenging. This highlights an urgent need to establish a reliable platform for assessing organ-specific toxicity in such supplements. In this study, we utilized a kidney organoid model derived from adult rat kidney stem cells (KS cells) to assess the potential tubular toxicity of these supplements. Methods: KS cell clusters were cultured in three-dimensional system supplemented with growth factors to promote kidney organoids. The organoids were subsequently exposed to Beni-koji CholesteHelp supplements or cisplatin, followed by histological and molecular analyses to evaluate structural impacts. Results: Established organoids had the kidney-like structures including tubular-like structures and glomerulus-like structures at the tips of multiple tubules. Treatment with Beni-koji CholesteHelp supplements induced significant tubular damage in the organoids, characterized by epithelial cell thinning, structural disruption, and increase in cleaved-caspase 3-positive apoptotic tubular cells, similar to the organoids treated with cisplatin. Conclusion: These findings provide the first evidence suggesting that certain toxicants in specific batches of Beni-koji CholesteHelp supplements cause direct renal tubular injury. This KS cell-based organoid system represents a cost-effective, reproducible, and technically simple platform for nephrotoxicity screening.
en-copyright=
kn-copyright=

en-aut-name=NakanohHiroyuki
en-aut-sei=Nakanoh
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsujiKenji
en-aut-sei=Tsuji
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FukushimaKazuhiko
en-aut-sei=Fukushima
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HaraguchiSoichiro
en-aut-sei=Haraguchi
en-aut-mei=Soichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KitamuraShinji
en-aut-sei=Kitamura
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Acute kidney injury
kn-keyword=Acute kidney injury
en-keyword=Drug-induced nephrotoxicity
kn-keyword=Drug-induced nephrotoxicity
en-keyword=Kidney organoid
kn-keyword=Kidney organoid
en-keyword=Kidney stem cell
kn-keyword=Kidney stem cell
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=3
article-no=
start-page=96
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250204
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cancer-associated fibroblasts promote pro-tumor functions of neutrophils in pancreatic cancer via IL-8: potential suppression by pirfenidone
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background The mechanisms by which neutrophils acquire pro-tumor properties remain poorly understood. In pancreatic cancer, cancer-associated fibroblasts (CAFs) may interact with neutrophils, directing them to promote tumor progression.<br>
Methods To validate the association between CAFs and neutrophils, the localization of neutrophils was examined in clinically resected pancreatic cancer specimens. CAFs were produced by culturing in cancer-conditioned media, and the effects of these CAFs on neutrophils were examined. In vitro migration and invasion assays assess the effect of CAF-activated neutrophils on cancer cells. The factors secreted by the activated neutrophils were also explored. Finally, pirfenidone (PFD) was tested to determine whether it could suppress the pro-tumor functions of activated neutrophils.<br>
Results In pancreatic cancer specimens, neutrophils tended to co-localize with IL-6-positive CAFs. Neutrophils co-cultured with CAFs increased migratory capacity and prolonged life span. CAF-affected neutrophils enhance the migratory and invasive activities of pancreatic cancer cells. IL-8 is the most upregulated cytokine secreted by the neutrophils. PFD suppresses IL-8 secretion from CAF-stimulated neutrophils and mitigates the malignant traits of pancreatic cancer cells.<br>
Conclusion CAFs activate neutrophils and enhance the malignant phenotype of pancreatic cancer. The interactions between cancer cells, CAFs, and neutrophils can be disrupted by PFD, highlighting a potential therapeutic approach.
en-copyright=
kn-copyright=

en-aut-name=YagiTomohiko
en-aut-sei=Yagi
en-aut-mei=Tomohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KagawaShunsuke
en-aut-sei=Kagawa
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NogiShohei
en-aut-sei=Nogi
en-aut-mei=Shohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TaniguchiAtsuki
en-aut-sei=Taniguchi
en-aut-mei=Atsuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshimotoMasashi
en-aut-sei=Yoshimoto
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SuemoriKanto
en-aut-sei=Suemori
en-aut-mei=Kanto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NagaiYasuo
en-aut-sei=Nagai
en-aut-mei=Yasuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=FujitaShuto
en-aut-sei=Fujita
en-aut-mei=Shuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KurodaShinji
en-aut-sei=Kuroda
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KikuchiSatoru
en-aut-sei=Kikuchi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KakiuchiYoshihiko
en-aut-sei=Kakiuchi
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=TeraishiFuminori
en-aut-sei=Teraishi
en-aut-mei=Fuminori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=TakagiKosei
en-aut-sei=Takagi
en-aut-mei=Kosei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=OharaToshiaki
en-aut-sei=Ohara
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=TazawaHiroshi
en-aut-sei=Tazawa
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

affil-num=1
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Departments of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Cancer-associated fibroblasts
kn-keyword=Cancer-associated fibroblasts
en-keyword=Neutrophil
kn-keyword=Neutrophil
en-keyword=Anti-fibrotic agent
kn-keyword=Anti-fibrotic agent
en-keyword=Pirfenidone
kn-keyword=Pirfenidone
END

start-ver=1.4
cd-journal=joma
no-vol=24
cd-vols=
no-issue=1
article-no=
start-page=1099
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240916
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Histological differences related to autophagy in the minor salivary gland between primary and secondary types of Sjögren's syndrome
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Some forms of Sjögren’s syndrome (SS) follow a clinical course accompanied by systemic symptoms caused by lymphocyte infiltration and proliferation in the liver, kidneys, and other organs. To better understand the clinical outcomes of SS, here we used minor salivary gland tissues from patients and examine their molecular, biological, and pathological characteristics. A retrospective study was performed, combining clinical data and formalin-fixed paraffin-embedded (FFPE) samples from female patients over 60 years of age who underwent biopsies at Okayama University Hospital. We employed direct digital RNA counting with nCounter® and multiplex immunofluorescence analysis with a PhenoCycler™ on the labial gland biopsies. We compared FFPE samples from SS patients who presented with other connective tissue diseases (secondary SS) with those from stable SS patients with symptoms restricted to the exocrine glands (primary SS). Secondary SS tissues showed enhanced epithelial damage and lymphocytic infiltration accompanied by elevated expression of autophagy marker genes in the immune cells of the labial glands. The close intercellular distance between helper T cells and B cells positive for autophagy-associated molecules suggests accelerated autophagy in these lymphocytes and potential B cell activation by helper T cells. These findings indicate that examination of FFPE samples from labial gland biopsies can be an effective tool for evaluating molecular histological differences between secondary and primary SS through multiplexed analysis of gene expression and tissue imaging.
en-copyright=
kn-copyright=

en-aut-name=Ono-MinagiHitomi
en-aut-sei=Ono-Minagi
en-aut-mei=Hitomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NohnoTsutomu
en-aut-sei=Nohno
en-aut-mei=Tsutomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakabatakeKiyofumi
en-aut-sei=Takabatake
en-aut-mei=Kiyofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TanakaTakehiro
en-aut-sei=Tanaka
en-aut-mei=Takehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KatsuyamaTakayuki
en-aut-sei=Katsuyama
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MiyawakiKohta
en-aut-sei=Miyawaki
en-aut-mei=Kohta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IbaragiSoichiro
en-aut-sei=Ibaragi
en-aut-mei=Soichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=IidaSeiji
en-aut-sei=Iida
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=YoshinoTadashi
en-aut-sei=Yoshino
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NagatsukaHitoshi
en-aut-sei=Nagatsuka
en-aut-mei=Hitoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=SakaiTakayoshi
en-aut-sei=Sakai
en-aut-mei=Takayoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=OhuchiHideyo
en-aut-sei=Ohuchi
en-aut-mei=Hideyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=Department of Cytology and Histology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
kn-affil=

affil-num=2
en-affil=Department of Cytology and Histology, Okayama University Medical School
kn-affil=

affil-num=3
en-affil=Department of Oral Pathology and Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Pathology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Hospital
kn-affil=

affil-num=6
en-affil=Division of Precision Medicine, Kyushu University School of Medicine
kn-affil=

affil-num=7
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=9
en-affil=Department of Oral and Maxillofacial Reconstructive Surgery, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=10
en-affil=Department of Pathology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
kn-affil=

affil-num=11
en-affil=Department of Oral Pathology and Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=12
en-affil=Department of Rehabilitation for Orofacial Disorders, Osaka University Graduate School of Dentistry
kn-affil=

affil-num=13
en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=Autoimmune disease
kn-keyword=Autoimmune disease
en-keyword=Xerostomia
kn-keyword=Xerostomia
en-keyword=Multiplex immunostaining
kn-keyword=Multiplex immunostaining
en-keyword=Spatial analysis
kn-keyword=Spatial analysis
en-keyword=Autophagy
kn-keyword=Autophagy
END

start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=10
article-no=
start-page=807
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240509
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Exploring the Regulators of Keratinization: Role of BMP-2 in Oral Mucosa
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The oral mucosa functions as a physico-chemical and immune barrier to external stimuli, and an adequate width of the keratinized mucosa around the teeth or implants is crucial to maintaining them in a healthy and stable condition. In this study, for the first time, bulk RNA-seq analysis was performed to explore the gene expression of laser microdissected epithelium and lamina propria from mice, aiming to investigate the differences between keratinized and non-keratinized oral mucosa. Based on the differentially expressed genes (DEGs) and Gene Ontology (GO) Enrichment Analysis, bone morphogenetic protein 2 (BMP-2) was identified to be a potential regulator of oral mucosal keratinization. Monoculture and epithelial-mesenchymal cell co-culture models in the air-liquid interface (ALI) indicated that BMP-2 has direct and positive effects on epithelial keratinization and proliferation. We further performed bulk RNA-seq of the ALI monoculture stimulated with BMP-2 in an attempt to identify the downstream factors promoting epithelial keratinization and proliferation. Analysis of the DEGs identified, among others, IGF2, ID1, LTBP1, LOX, SERPINE1, IL24, and MMP1 as key factors. In summary, these results revealed the involvement of a well-known growth factor responsible for bone development, BMP-2, in the mechanism of oral mucosal keratinization and proliferation, and pointed out the possible downstream genes involved in this mechanism.
en-copyright=
kn-copyright=

en-aut-name=MuXindi
en-aut-sei=Mu
en-aut-mei=Xindi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OnoMitsuaki
en-aut-sei=Ono
en-aut-mei=Mitsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NguyenHa Thi Thu
en-aut-sei=Nguyen
en-aut-mei=Ha Thi Thu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WangZiyi
en-aut-sei=Wang
en-aut-mei=Ziyi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=ZhaoKun
en-aut-sei=Zhao
en-aut-mei=Kun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KomoriTaishi
en-aut-sei=Komori
en-aut-mei=Taishi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YonezawaTomoko
en-aut-sei=Yonezawa
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KubokiTakuo
en-aut-sei=Kuboki
en-aut-mei=Takuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OohashiToshitaka
en-aut-sei=Oohashi
en-aut-mei=Toshitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=
kn-affil=Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=7
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Oral Rehabilitation and Implantology, Okayama University Hospital
kn-affil=

affil-num=9
en-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=cell differentiation
kn-keyword=cell differentiation
en-keyword=epithelia
kn-keyword=epithelia
en-keyword=growth factor(s)
kn-keyword=growth factor(s)
en-keyword=bioinformatics
kn-keyword=bioinformatics
en-keyword=extracellular matrix (ECM)
kn-keyword=extracellular matrix (ECM)
en-keyword=mucocutaneous disorders
kn-keyword=mucocutaneous disorders
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=11
article-no=
start-page=3787
end-page=3802
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230905
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=PD-L1-expressing cancer-associated fibroblasts induce tumor immunosuppression and contribute to poor clinical outcome in esophageal cancer
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The programmed cell death 1 protein (PD-1)/programmed cell death ligand 1 (PD-L1) axis plays a crucial role in tumor immunosuppression, while the cancer-associated fibroblasts (CAFs) have various tumor-promoting functions. To determine the advantage of immunotherapy, the relationship between the cancer cells and the CAFs was evaluated in terms of the PD-1/PD-L1 axis. Overall, 140 cases of esophageal cancer underwent an immunohistochemical analysis of the PD-L1 expression and its association with the expression of the α smooth muscle actin, fibroblast activation protein, CD8, and forkhead box P3 (FoxP3) positive cells. The relationship between the cancer cells and the CAFs was evaluated in vitro, and the effect of the anti-PD-L1 antibody was evaluated using a syngeneic mouse model. A survival analysis showed that the PD-L1+ CAF group had worse survival than the PD-L1- group. In vitro and in vivo, direct interaction between the cancer cells and the CAFs showed a mutually upregulated PD-L1 expression. In vivo, the anti-PD-L1 antibody increased the number of dead CAFs and cancer cells, resulting in increased CD8+ T cells and decreased FoxP3+ regulatory T cells. We demonstrated that the PD-L1-expressing CAFs lead to poor outcomes in patients with esophageal cancer. The cancer cells and the CAFs mutually enhanced the PD-L1 expression and induced tumor immunosuppression. Therefore, the PD-L1-expressing CAFs may be good targets for cancer therapy, inhibiting tumor progression and improving host tumor immunity.
en-copyright=
kn-copyright=

en-aut-name=KawasakiKento
en-aut-sei=Kawasaki
en-aut-mei=Kento
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NomaKazuhiro
en-aut-sei=Noma
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatoTakuya
en-aut-sei=Kato
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OharaToshiaki
en-aut-sei=Ohara
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TanabeShunsuke
en-aut-sei=Tanabe
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakedaYasushige
en-aut-sei=Takeda
en-aut-mei=Yasushige
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MatsumotoHijiri
en-aut-sei=Matsumoto
en-aut-mei=Hijiri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NishimuraSeitaro
en-aut-sei=Nishimura
en-aut-mei=Seitaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KunitomoTomoyoshi
en-aut-sei=Kunitomo
en-aut-mei=Tomoyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=AkaiMasaaki
en-aut-sei=Akai
en-aut-mei=Masaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KobayashiTeruki
en-aut-sei=Kobayashi
en-aut-mei=Teruki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=NishiwakiNoriyuki
en-aut-sei=Nishiwaki
en-aut-mei=Noriyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KashimaHajime
en-aut-sei=Kashima
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=MaedaNaoaki
en-aut-sei=Maeda
en-aut-mei=Naoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=KikuchiSatoru
en-aut-sei=Kikuchi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=TazawaHiroshi
en-aut-sei=Tazawa
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=ShirakawaYasuhiro
en-aut-sei=Shirakawa
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

affil-num=1
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=17
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=18
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Esophageal cancer
kn-keyword=Esophageal cancer
en-keyword=Cancer-associated fibroblasts
kn-keyword=Cancer-associated fibroblasts
en-keyword=Programmed cell death 1
kn-keyword=Programmed cell death 1
en-keyword=Program cell death ligand 1
kn-keyword=Program cell death ligand 1
en-keyword=Immune checkpoint inhibitors
kn-keyword=Immune checkpoint inhibitors
END

start-ver=1.4
cd-journal=joma
no-vol=78
cd-vols=
no-issue=2
article-no=
start-page=107
end-page=113
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=202404
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Evaluation of the Efficacy and Safety of Tenofovir Disoproxil Fumarate in Intercepting Mother-to-Child Transmission of Hepatitis B Virus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vertical transmission of hepatitis B virus (HBV), especially in Asia, is a key target in the global elimination of HBV. This study assessed the effects of tenofovir disoproxil fumarate (TDF) in pregnant women for mother-to-infant transmission of HBV. A total of 122 pregnant women at our hospital met the inclusion criteria for high HBV DNA viral loads. They were randomly divided into TDF-treatment (n=70) and placebo (n=52) groups. Maternal liver function and serum HBV DNA load were tested before and after treatment. Clinical and laboratory data of infants were assayed at delivery and 7-months post-partum visit and compared between the two groups. There was no difference in clinical characteristics of participants between the two groups. There were no significant differences in liver function markers, including alanine aminotransferase, total bilirubin, blood creatinine, and blood urea nitrogen levels before and after TDF treatment. The serum HBV DNA viral load of the TDF-treated group became significantly lower than those of the control group and their own pre-medication levels. Infants showed no significant difference in body growth, including weight, height, head size, and five-min Apgar score. At 7 months after birth, 94.29% of infants in the TDF group and 86.54% of control-group infants had protective HBsAb levels ≥ 10 mIU/ml (p>0.05). The HBV infection rate of infants in the TDF-treated group was lower than that in the non-treated group. In high-HBV-DNA-load pregnant women, TDF administered from 28 weeks gestational age to delivery was associated with a lower risk of mother-to-infant transmission of HBV.
en-copyright=
kn-copyright=

en-aut-name=HanDongxiang
en-aut-sei=Han
en-aut-mei=Dongxiang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=DuJianxiu
en-aut-sei=Du
en-aut-mei=Jianxiu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WangWei
en-aut-sei=Wang
en-aut-mei=Wei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WangCui
en-aut-sei=Wang
en-aut-mei=Cui
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Obstetrics, Shijiazhuang Maternity & Child Healthcare Hospital
kn-affil=

affil-num=2
en-affil=Department of Laboratory Medicine, Shijiazhuang Maternity & Child Healthcare Hospital
kn-affil=

affil-num=3
en-affil=Department of Obstetrics, Shijiazhuang Maternity & Child Healthcare Hospital
kn-affil=

affil-num=4
en-affil=Department of Functional, Shijiazhuang Maternity & Child Healthcare Hospital
kn-affil=
en-keyword=mother-to-infant transmission
kn-keyword=mother-to-infant transmission
en-keyword=tenofovir disoproxil fumarate
kn-keyword=tenofovir disoproxil fumarate
en-keyword=hepatitis B virus
kn-keyword=hepatitis B virus
END

start-ver=1.4
cd-journal=joma
no-vol=47
cd-vols=
no-issue=2
article-no=
start-page=589
end-page=596
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240219
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Evaluation of the effect of sagging correction calibration errors in radiotherapy software on image matching
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=To investigate the impact of sagging correction calibration errors in radiotherapy software on image matching. Three software applications were used, with and without a polymethyl methacrylate rod supporting the ball bearings (BB). The calibration error for sagging correction across nine flex maps (FMs) was determined by shifting the BB positions along the Left–Right (LR), Gun–Target (GT), and Up–Down (UD) directions from the reference point. Lucy and pelvic phantom cone-beam computed tomography (CBCT) images underwent auto-matching after modifying each FM. Image deformation was assessed in orthogonal CBCT planes, and the correlations among BB shift magnitude, deformation vector value, and differences in auto-matching were analyzed. The average difference in analysis results among the three softwares for the Winston–Lutz test was within 0.1 mm. The determination coefficients (R2) between the BB shift amount and Lucy phantom matching error in each FM were 0.99, 0.99, and 1.00 in the LR-, GT-, and UD-directions, respectively. The pelvis phantom demonstrated no cross-correlation in the GT direction during auto-matching error evaluation using each FM. The correlation coefficient (r) between the BB shift and the deformation vector value was 0.95 on average for all image planes. Slight differences were observed among software in the evaluation of the Winston–Lutz test. The sagging correction calibration error in the radiotherapy imaging system was caused by an auto-matching error of the phantom and deformation of CBCT images.
en-copyright=
kn-copyright=

en-aut-name=YamazawaYumi
en-aut-sei=Yamazawa
en-aut-mei=Yumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OsakaAkitane
en-aut-sei=Osaka
en-aut-mei=Akitane
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FujiiYasushi
en-aut-sei=Fujii
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NakayamaTakahiro
en-aut-sei=Nakayama
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NishiokaKunio
en-aut-sei=Nishioka
en-aut-mei=Kunio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanabeYoshinori
en-aut-sei=Tanabe
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Radiology, Niigata Prefectural Central Hospital
kn-affil=

affil-num=2
en-affil=Department of Radiology, Niigata Prefectural Central Hospital
kn-affil=

affil-num=3
en-affil=Department of Radiology, Chugoku Central Hospital of the Mutual Aid Association of Public School Teachers
kn-affil=

affil-num=4
en-affil=Department of Radiology, Chugoku Central Hospital of the Mutual Aid Association of Public School Teachers
kn-affil=

affil-num=5
en-affil=Department of Radiology, Tokuyama Central Hospital
kn-affil=

affil-num=6
en-affil=Faculty of Medicine, Graduate School of Health Sciences, Okayama University
kn-affil=
en-keyword=Radiotherapy
kn-keyword=Radiotherapy
en-keyword=Sagging correction
kn-keyword=Sagging correction
en-keyword=Image matching
kn-keyword=Image matching
en-keyword=Winston-Lutz test
kn-keyword=Winston-Lutz test
en-keyword=Deformable registration
kn-keyword=Deformable registration
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=6
article-no=
start-page=723
end-page=730
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=202212
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Serum miR-377 Can Be Used as a Diagnostic Marker for Acute Coronary Syndrome and Can Regulate Proinflammatory Factors and Endothelial Injury Markers
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The diagnostic value of microRNA-377 (miR-377) in patients with acute coronary syndrome (ACS) and explored miR-377’s potential mechanisms. We performed an qRT-PCR to assess serum miR-377 levels in ACS patients and coronary artery ligation rat models. The diagnostic value of miR-377 was evaluated by determining the ROC curve. An ELISA assay was conducted to detect the model rat endothelial damage markers von Willebrand factor (vWF) and heart-type fatty acid binding protein (H-FABP), and proinflammatory cytokines TNF-α, IL-6, and IL-1β. The serum miR-377 level was elevated in the ACS patients and significantly increased in the ACS rats. MiR-377 has a high diagnostic value in ACS patients, with a 0.844 ROC, 76.47% specificity, and 87.10% sensitivity. MiR-377 was positively correlated with the expressions of vWF, H-FABP, cTnI, TNF-α, IL-6, and IL-1β. In ACS rats, reducing the expression of miR-377 significantly inhibited the increases in vWF, H-FABP, TNF-α, IL-6, and IL-1β. An elevated miR-377 level can be used as a diagnostic marker in patients with ACS. A reduction of miR-377 may alleviate ACS by improving myocardial damage such as endothelial injury and the inflammatory response.
en-copyright=
kn-copyright=

en-aut-name=ZhangQuan
en-aut-sei=Zhang
en-aut-mei=Quan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YangLixia
en-aut-sei=Yang
en-aut-mei=Lixia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WanGuozhen
en-aut-sei=Wan
en-aut-mei=Guozhen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ZhangXiaoqiang
en-aut-sei=Zhang
en-aut-mei=Xiaoqiang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WangYing
en-aut-sei=Wang
en-aut-mei=Ying
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ZhaoGuannan
en-aut-sei=Zhao
en-aut-mei=Guannan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Affiliated Hospital of Gansu Medical College
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Medicine, Affiliated Hospital of Gansu Medical College
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular Medicine, Affiliated Hospital of Gansu Medical College
kn-affil=

affil-num=4
en-affil=Department of Cardiovascular Medicine, Affiliated Hospital of Gansu Medical College
kn-affil=

affil-num=5
en-affil=Department of Cardiovascular Medicine, Affiliated Hospital of Gansu Medical College
kn-affil=

affil-num=6
en-affil=Department of Dermatological, Pingliang Traditional Chinese Medicine Hospital
kn-affil=
en-keyword=microRNA-377
kn-keyword=microRNA-377
en-keyword=acute coronary syndrome
kn-keyword=acute coronary syndrome
en-keyword=diagnosis
kn-keyword=diagnosis
en-keyword=endothelial injury
kn-keyword=endothelial injury
en-keyword=inflammatory
kn-keyword=inflammatory
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=5
article-no=
start-page=489
end-page=502
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=202210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Current Insights into Mesenchymal Signatures in Glioblastoma
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Glioblastoma (GBM) is a fatal primary malignant brain tumor in adults. Despite decades of research, the prognosis for GBM patients is still disappointing. One major reason for the intense therapeutic resistance of GBM is inter- and intra-tumor heterogeneity. GBM-intrinsic transcriptional profiling has suggested the presence of at least three subtypes of GBM: the proneural, classic, and mesenchymal subtypes. The mesenchymal subtype is the most aggressive, and patients with the mesenchymal subtype of primary and recurrent tumors tend to have a worse prognosis compared with patients with the other subtypes. Furthermore, GBM can shift from other subtypes to the mesenchymal subtype over the course of disease progression or recurrence. This phenotypic transition is driven by diverse tumor-intrinsic molecular mechanisms or microenvironmental factors. Thus, better understanding of the plastic nature of mesenchymal transition in GBM is pivotal to developing new therapeutic strategies. In this review, we provide a comprehensive overview of the current understanding of the elements involved in the mesenchymal transition of GBM and discuss future perspectives.
en-copyright=
kn-copyright=

en-aut-name=MatsumotoYuji
en-aut-sei=Matsumoto
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IchikawaTomotsugu
en-aut-sei=Ichikawa
en-aut-mei=Tomotsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurozumiKazuhiko
en-aut-sei=Kurozumi
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=DateIsao
en-aut-sei=Date
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Neurological Surgery, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=3
en-affil=Department of Neurosurgery, Hamamatsu University Hospital
kn-affil=

affil-num=4
en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=glioma
kn-keyword=glioma
en-keyword=glioblastoma
kn-keyword=glioblastoma
en-keyword=mesenchymal subtype
kn-keyword=mesenchymal subtype
en-keyword=mesenchymal transition
kn-keyword=mesenchymal transition
en-keyword=heterogeneity
kn-keyword=heterogeneity
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=22
article-no=
start-page=13917
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=2022
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Droplet motion on a wrinkled PDMS surface with a gradient structural length scale shorter than the droplet diameter
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Droplet transportation using a wettability gradient surface has attracted much attention owing to applications such as in microfluidic devices. A surface with a spatial structural gradient was prepared through a simple and cost-effective process even though understanding of droplet behavior on the structure was still limited. Here, we report impinging droplet motion on a gradient wrinkled surface. Surfaces were prepared through hard film deposition on soft pre-strained polydimethylsiloxane (PDMS) with a mask installed with a slit to control the amount of deposition, which is related to the wavelength of the wrinkles. Droplets were impinged with varying position with respect to the structure, and the droplet motion was observed in the direction away from the region under the slit. We found an asymmetric contact angle and alternate motion on both sides of the three-phase contact line during the motion according to the gradient of the wrinkle wavelength. These results may help not only to understand the behavior of droplet impingement on a gradient structural surface but also to further develop applications using directional droplet transfer.
en-copyright=
kn-copyright=

en-aut-name=YamadaYutaka
en-aut-sei=Yamada
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IsobeKazuma
en-aut-sei=Isobe
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HoribeAkihiko
en-aut-sei=Horibe
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=12
article-no=
start-page=3286
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210614
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Biological Effects of Bioresorbable Materials in Alveolar Ridge Augmentation: Comparison of Early and Slow Resorbing Osteosynthesis Materials
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The purpose of this study was to investigate the bone healing properties and histological environment of a u-HA/PLLA/PGA (u-HA-uncalcined and unsintered hydroxyapatite, PLLA-Poly L-lactic acid, PGA-polyglycolic acid) composite device in humans, and to understand the histological dynamics of using this device for maxillofacial treatments. Twenty-one subjects underwent pre-implant maxillary alveolar ridge augmentation with mandibular cortical bone blocks using u-HA/PLLA or u-HA/PLLA/PGA screws for fixation. Six months later, specimens of these screws and their adjacent tissue were retrieved. A histological and immunohistochemical evaluation of these samples was performed using collagen 1a, ALP (alkaline phosphatase), and osteocalcin. We observed that alveolar bone augmentation was successful for all of the subjects. Upon histological evaluation, the u-HA/PLLA screws had merged with the bone components, and the bone was directly connected to the biomaterial. In contrast, direct bone connection was not observed for the u-HA/PLLA/PGA screw. Immunohistological findings showed that in the u-HA/PLLA group, collagen 1a was positive for fibers that penetrated vertically into the bone. Alkaline phosphatase was positive only in the u-HA/PLLA stroma, and the stroma was negative for osteocalcin. In this study, u-HA/PLLA showed a greater bioactive bone conductivity than u-HA/PLLA/PGA and a higher biocompatibility for direct bone attachment. Furthermore, u-HA/PLLA was shown to have the potential for bone formation in the stroma.
en-copyright=
kn-copyright=

en-aut-name=KawaiHotaka
en-aut-sei=Kawai
en-aut-mei=Hotaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SukegawaShintaro
en-aut-sei=Sukegawa
en-aut-mei=Shintaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakanoKeisuke
en-aut-sei=Nakano
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakabatakeKiyofumi
en-aut-sei=Takabatake
en-aut-mei=Kiyofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OnoSawako
en-aut-sei=Ono
en-aut-mei=Sawako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NagatsukaHitoshi
en-aut-sei=Nagatsuka
en-aut-mei=Hitoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=FurukiYoshihiko
en-aut-sei=Furuki
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Pathology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=6
en-affil=Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Oral and Maxillofacial Surgery, Kagawa Prefectural Central Hospital
kn-affil=
en-keyword=poly L-lactic acid
kn-keyword=poly L-lactic acid
en-keyword=uncalcined and unsintered hydroxyapatite
kn-keyword=uncalcined and unsintered hydroxyapatite
en-keyword=polyglycolic acid
kn-keyword=polyglycolic acid
en-keyword=alveolar ridge augmentation
kn-keyword=alveolar ridge augmentation
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210107
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=VEGFR2 blockade augments the effects of tyrosine kinase inhibitors by inhibiting angiogenesis and oncogenic signaling in oncogene-driven non-small-cell lung cancers
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Molecular agents targeting the epidermal growth factor receptor (EGFR)-, anaplastic lymphoma kinase (ALK)- or c-ros oncogene 1 (ROS1) alterations have revolutionized the treatment of oncogene-driven non-small-cell lung cancer (NSCLC). However, the emergence of acquired resistance remains a significant challenge, limiting the wider clinical success of these molecular targeted therapies. In this study, we investigated the efficacy of various molecular targeted agents, including erlotinib, alectinib, and crizotinib, combined with anti-vascular endothelial growth factor receptor (VEGFR) 2 therapy. The combination of VEGFR2 blockade with molecular targeted agents enhanced the anti-tumor effects of these agents in xenograft mouse models of EGFR-, ALK-, or ROS1-altered NSCLC. The numbers of CD31-positive blood vessels were significantly lower in the tumors of mice treated with an anti-VEGFR2 antibody combined with molecular targeted agents compared with in those of mice treated with molecular targeted agents alone, implying the antiangiogenic effects of VEGFR2 blockade. Additionally, the combination therapies exerted more potent antiproliferative effects in vitro in EGFR-, ALK-, or ROS1-altered NSCLC cells, implying that VEGFR2 inhibition also has direct anti-tumor effects on cancer cells. Furthermore, VEGFR2 expression was induced following exposure to molecular targeted agents, implying the importance of VEGFR2 signaling in NSCLC patients undergoing molecular targeted therapy. In conclusion, VEGFR2 inhibition enhanced the anti-tumor effects of molecular targeted agents in various oncogene-driven NSCLC models, not only by inhibiting tumor angiogenesis but also by exerting direct antiproliferative effects on cancer cells. Hence, combination therapy with anti-VEGFR2 antibodies and molecular targeted agents could serve as a promising treatment strategy for oncogene-driven NSCLC.
en-copyright=
kn-copyright=

en-aut-name=WatanabeHiromi
en-aut-sei=Watanabe
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IchiharaEiki
en-aut-sei=Ichihara
en-aut-mei=Eiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KayataniHiroe
en-aut-sei=Kayatani
en-aut-mei=Hiroe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MakimotoGo
en-aut-sei=Makimoto
en-aut-mei=Go
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NinomiyaKiichiro
en-aut-sei=Ninomiya
en-aut-mei=Kiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NishiiKazuya
en-aut-sei=Nishii
en-aut-mei=Kazuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HigoHisao
en-aut-sei=Higo
en-aut-mei=Hisao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=AndoChihiro
en-aut-sei=Ando
en-aut-mei=Chihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OkawaSachi
en-aut-sei=Okawa
en-aut-mei=Sachi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=NakasukaTakamasa
en-aut-sei=Nakasuka
en-aut-mei=Takamasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KanoHirohisa
en-aut-sei=Kano
en-aut-mei=Hirohisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=HaraNaofumi
en-aut-sei=Hara
en-aut-mei=Naofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=HirabaeAtsuko
en-aut-sei=Hirabae
en-aut-mei=Atsuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=KatoYuka
en-aut-sei=Kato
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=NinomiyaTakashi
en-aut-sei=Ninomiya
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=KuboToshio
en-aut-sei=Kubo
en-aut-mei=Toshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=RaiKammei
en-aut-sei=Rai
en-aut-mei=Kammei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=OhashiKadoaki
en-aut-sei=Ohashi
en-aut-mei=Kadoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=HottaKatsuyuki
en-aut-sei=Hotta
en-aut-mei=Katsuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=TabataMasahiro
en-aut-sei=Tabata
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=MaedaYoshinobu
en-aut-sei=Maeda
en-aut-mei=Yoshinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=KiuraKatsuyuki
en-aut-sei=Kiura
en-aut-mei=Katsuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

affil-num=1
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Allergy and Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=15
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Center for Clinical Oncology, Okayama University Hospital
kn-affil=

affil-num=17
en-affil=Department of Allergy and Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=18
en-affil=Department of Allergy and Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=19
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=20
en-affil=Center for Clinical Oncology, Okayama University Hospital
kn-affil=

affil-num=21
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=22
en-affil=Department of Allergy and Respiratory Medicine, Okayama University Hospital
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=559
cd-vols=
no-issue=
article-no=
start-page=119928
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210105
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Experimental variable effects on laser heating of inclusions during Raman spectroscopic analysis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Raman spectroscopy for fluid, melt, and mineral inclusions provides direct insight into the physicochemical conditions of the environment surrounding the host mineral at the time of trapping. However, the obtained Raman spectral characteristics such as peak position are modified because of local temperature enhancement of the inclusions by the excitation laser, which might engender systematic errors and incorrect conclusions if the effect is not corrected. Despite the potentially non-negligible effects of laser heating, the laser heating coefficient (B) (°C/mW) of inclusions has remained unsolved. For this study, we found B from experiments and heat transport simulation to evaluate how various parameters such as experimental conditions, mineral properties, and inclusion geometry affect B of inclusions. To assess the parameters influencing laser heating, we measured B of a total of 19 CO2-rich fluid inclusions hosted in olivine, orthopyroxene, clinopyroxene, spinel, and quartz. Our results revealed that the measured B of fluid inclusions in spinel is highest (approx. 6 °C/mW) and that of quartz is lowest (approx. 1 × 10−2 °C/mW), consistent with earlier inferences. Our simulation results show that the absorption coefficient of the host mineral is correlated linearly with B. It is the most influential parameter when the absorption coefficient of the host mineral (αh) is larger than that of an inclusion (αinc). Furthermore, although our results indicate that both the inclusion size and depth have little effect on B if αh > αinc, the thickness and radius of the host mineral slightly influence B. These results suggest that the choice of inclusion size and depth to be analyzed in a given sample do not cause any systematic error in the Raman data because of laser heating, but the host radius and thickness, which can be adjusted to some degree at the time of sample preparation, can cause systematic errors between samples.Our results demonstrate that, even with laser power of 10 mW, which is typical for inclusion analysis, the inclusion temperature rises to tens or hundreds of degrees during the analysis, depending especially on the host mineral geometry and optical properties. Therefore, correction of the heating effects will be necessary to obtain reliable data from Raman spectroscopic analysis of inclusions. This paper presents some correction methods for non-negligible effects of laser heating.
en-copyright=
kn-copyright=

en-aut-name=HagiwaraYuuki
en-aut-sei=Hagiwara
en-aut-mei=Yuuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YoshidaKenta
en-aut-sei=Yoshida
en-aut-mei=Kenta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YonedaAkira
en-aut-sei=Yoneda
en-aut-mei=Akira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TorimotoJunji
en-aut-sei=Torimoto
en-aut-mei=Junji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YamamotoJunji
en-aut-sei=Yamamoto
en-aut-mei=Junji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Graduate School of Science, Hokkaido University
kn-affil=

affil-num=2
en-affil=Research Institute for Marine Geodynamics, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
kn-affil=

affil-num=3
en-affil=Institute for Planetary Materials, Okayama University
kn-affil=

affil-num=4
en-affil=Ore Genesis Research Unit, Project Team for Development of New-Generation Research Protocol for Submarine Resources, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
kn-affil=

affil-num=5
en-affil=The Hokkaido University Museum
kn-affil=
en-keyword=Finite element method
kn-keyword=Finite element method
en-keyword=Inclusions
kn-keyword=Inclusions
en-keyword=Laser heating
kn-keyword=Laser heating
en-keyword=Raman spectroscopy
kn-keyword=Raman spectroscopy
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=1
article-no=
start-page=53
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201031
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effectiveness of impedance parameters for muscle quality evaluation in healthy men
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We investigated the relationship between impedance parameters and skeletal muscle function in the lower extremities, as well as the effectiveness of impedance parameters in evaluating muscle quality. Lower extremity impedance of 19 healthy men (aged 23–31 years) measured using the direct segmental multi-frequency bioelectrical impedance analysis were arc-optimized using the Cole–Cole model, following which phase angle (PA), Ri/Re, and β were estimated. Skeletal muscle function was assessed by muscle thickness, muscle intensity, and isometric knee extension force (IKEF). IKEF was positively correlated with PA (r = 0.58, p < 0.01) and β (r = 0.34, p < 0.05) was negatively correlated with Ri/Re (r = − 0.43, p < 0.01). Stepwise multiple regression analysis results revealed that PA, β, and Ri/Re were correlated with IKEF independently of muscle thickness. This study suggests that arc-optimized impedance parameters are effective for evaluating muscle quality and prediction of muscle strength.
en-copyright=
kn-copyright=

en-aut-name=SatoHiroki
en-aut-sei=Sato
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NakamuraTakao
en-aut-sei=Nakamura
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KusuharaToshimasa
en-aut-sei=Kusuhara
en-aut-mei=Toshimasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KenichiKobara
en-aut-sei=Kenichi
en-aut-mei=Kobara
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KuniyasuKatsushi
en-aut-sei=Kuniyasu
en-aut-mei=Katsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KawashimaTakaki
en-aut-sei=Kawashima
en-aut-mei=Takaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HanayamaKozo
en-aut-sei=Hanayama
en-aut-mei=Kozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of Radiological Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Radiological Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Radiological Technology, Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Physical Therapist, Faculty of Rehabilitation, Kawasaki University of Medical Welfare
kn-affil=

affil-num=5
en-affil=Department of Physical Therapist, Faculty of Rehabilitation, Kawasaki University of Medical Welfare
kn-affil=

affil-num=6
en-affil=Department of Physical Therapist, Kawasaki Junior College of Rehabilitation
kn-affil=

affil-num=7
en-affil=Department of Rehabilitation Medicine, Kawasaki Medical School, 577, Matsushim
kn-affil=
en-keyword=Phase angle
kn-keyword=Phase angle
en-keyword=Bioelectrical impedance analysis
kn-keyword=Bioelectrical impedance analysis
en-keyword=Cole–Cole model
kn-keyword=Cole–Cole model
en-keyword=Muscle quality
kn-keyword=Muscle quality
END

start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=1
article-no=
start-page=427
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201023
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Release and extraction of retained subfoveal perfluorocarbon liquid facilitated by subretinal BSS, vibration, and gravity: a case report
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background</br>
Perfluorocarbon liquid (PFCL) is an effective surgical adjuvant in performing vitrectomy for severe vitreoretinal pathologies such as proliferative vitreoretinopathy and giant retinal tears. However, subretinal retention of PFCL can occur postoperatively and retained PFCL causes severe visual disorders, particularly when PFCL was retained under the fovea. Although several procedures have been proposed for subfoveal PFCL removal, such as direct aspiration or submacular injection of balanced salt solution (BSS) to dislodge the subfoveal PFCL, the retinal damage associated with these procedures has been a major problem. Here, we report a case of subfoveal retention of PFCL for which we performed a novel surgical technique that attempts to minimize retinal damage.</br>
Case presentation</br>
A 69-year-old man presented with subfoveal retained PFCL after surgery for retinal detachment. To remove the retained PFCL, the internal limiting membrane overlying the subretinal injection site is first peeled to allow low-pressure (8 psi) transretinal BSS infusion, using a 41-gauge cannula, to slowly detach the macula. A small drainage retinotomy is created with the diathermy tip at the inferior position of the macular bleb, sized to be slightly wider than that of the PFCL droplet. The head of the bed is then raised, and the surgeon gently vibrates the patient’s head to release the PFCL droplet to allow it to migrate inferiorly towards the drainage retinotomy. The bed is returned to the horizontal position, and the PFCL, now on the retinal surface, can be aspirated. The subfoveal PFCL is removed while minimizing iatrogenic foveal and macular damage. One month after PFCL removal, the foveal structure showed partial recovery on optical coherence tomography, and BCVA improved to 20/40.</br>
Conclusion</br>
Creating a macular bleb with low infusion pressure and using vibrational forces and gravity to migrate the PFCL towards a retinotomy can be considered as a relatively atraumatic technique to remove subfoveal retained PFCL.
en-copyright=
kn-copyright=

en-aut-name=TakahashiKosuke
en-aut-sei=Takahashi
en-aut-mei=Kosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KimuraShuhei
en-aut-sei=Kimura
en-aut-mei=Shuhei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HosokawaMio Morizane
en-aut-sei=Hosokawa
en-aut-mei=Mio Morizane
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ShiodeYusuke
en-aut-sei=Shiode
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=DoiShinichiro
en-aut-sei=Doi
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MatobaRyo
en-aut-sei=Matoba
en-aut-mei=Ryo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KanzakiYuki
en-aut-sei=Kanzaki
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YonekawaYoshihiro
en-aut-sei=Yonekawa
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=MorizaneYuki
en-aut-sei=Morizane
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Wills Eye Hospital, Mid Atlantic Retina, Thomas Jefferson University
kn-affil=

affil-num=9
en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Case report
kn-keyword=Case report
en-keyword=Perfluorocarbon
kn-keyword=Perfluorocarbon
en-keyword=Retinal detachment
kn-keyword=Retinal detachment
en-keyword=Subretinal injection
kn-keyword=Subretinal injection
en-keyword=Vitreoretinal surgery
kn-keyword=Vitreoretinal surgery
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=5
article-no=
start-page=391
end-page=399
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=202010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Three-dimensional Evaluation of Abnormal Gait in Patients with Hip Osteoarthritis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Indexes for objectively evaluating abnormal gait in hip osteoarthritis (OA) patients and determining effective interventions are unclear. We analyzed the abnormal gait of hip OA patients by focusing on movements of the trunk and pelvis to establish an effective evaluation index for each direction of motion. We studied 28 patients with secondary hip OA due to developmental dysplasia of the hip and 16 controls. The trunk and pelvic movements during gait were measured in the medial-lateral (x), vertical (y), and back-and-forth (z) directions by a triaxial angular accelerometer. Gait speed, steps, step length, muscle strength, range of motion, and timed up-and-go (TUG) test performance were measured. We determined the correlations between physical function and the index of abnormal gait in the hip OA patients. Movements other than trunk and pelvic motions in the y-direction indicated abnormal gait in the patients. Significant correlations were found between abnormal gait and range of motions (extension, internal rotation), TUG score, stride length, and steps. The TUG test, stride length and steps were important for evaluating abnormal gait in hip OA patients. Individual interventions for each movement direction are required.
en-copyright=
kn-copyright=

en-aut-name=HommaDaisuke
en-aut-sei=Homma
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MinatoIzumi
en-aut-sei=Minato
en-aut-mei=Izumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ImaiNorio
en-aut-sei=Imai
en-aut-mei=Norio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MiyasakaDai
en-aut-sei=Miyasaka
en-aut-mei=Dai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SakaifYoshinori
en-aut-sei=Sakaif
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HorigomeYoji
en-aut-sei=Horigome
en-aut-mei=Yoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SuzukiHayato
en-aut-sei=Suzuki
en-aut-mei=Hayato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ShimadaHayato
en-aut-sei=Shimada
en-aut-mei=Hayato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=DohmaeYoichiro
en-aut-sei=Dohmae
en-aut-mei=Yoichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=EndoNaoto
en-aut-sei=Endo
en-aut-mei=Naoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=2
en-affil=Division of Orthopedic Surgery, Niigata Rinko Hospital
kn-affil=

affil-num=3
en-affil=Division of Comprehensive Geriatrics in Community, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=4
en-affil=Division of Orthopedic Surgery, Niigata Bandai Hospital
kn-affil=

affil-num=5
en-affil=Division of Orthopedic Surgery, Niigata General Hospital
kn-affil=

affil-num=6
en-affil=Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=7
en-affil=Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=

affil-num=8
en-affil=Division of Orthopedic Surgery, Mito Saiseikai General Hospital
kn-affil=

affil-num=9
en-affil=Division of Orthopedic Surgery, Niigata Bandai Hospital
kn-affil=

affil-num=10
en-affil=Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences
kn-affil=
en-keyword=hip osteoarthritis
kn-keyword=hip osteoarthritis
en-keyword=three-dimensional gait analysis
kn-keyword=three-dimensional gait analysis
en-keyword=abnormal gait
kn-keyword=abnormal gait
en-keyword=timed up and go test
kn-keyword=timed up and go test
en-keyword=range of motion
kn-keyword=range of motion
END

start-ver=1.4
cd-journal=joma
no-vol=138
cd-vols=
no-issue=
article-no=
start-page=111235
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200303
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of maternal bisphenol A diglycidyl ether exposure during gestation and lactation on behavior and brain development of the offspring
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bisphenol A diglycidyl ether (BADGE) is an epoxy resin used for the inner coating of canned food and beverages. BADGE can easily migrate from the containers and become a contaminant. In this study, we examined the effects of BADGE exposure to the dams on the behavioral, structural, and developmental abnormalities in the offspring. Female pregnant mice were fed with a diet containing BADGE (0.15 or 1.5 mg/kg/day) during gestation and lactation periods. In an open field test, the time spent in the corner area significantly increases in male mice of high-dose BADGE group at 5 weeks old. The histological analysis using offspring brain at postnatal day 1 delivered from BADGE (1.5 mg/kg/day)-treated dams demonstrates that positive signals of Forkhead box P2- and COUP-TF interacting protein 2 are restricted in each cortical layer, but not in the control brain. In addition, the maternal BADGE exposure reduces nestin-positive fibers of the radial glia and T-box transcription factor 2-positive intermediate progenitors in the inner subventricular zone. Furthermore, a direct BADGE exposure promotes neurite outgrowth and neuronal connection in the primary cultured cortical neurons. These data suggest that maternal BADGE exposure can accelerate neuronal differentiation in fetuses and induce anxiety-like behavior in juvenile mice.
en-copyright=
kn-copyright=

en-aut-name=MiyazakiIkuko
en-aut-sei=Miyazaki
en-aut-mei=Ikuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KikuokaRyo
en-aut-sei=Kikuoka
en-aut-mei=Ryo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=IsookaNami
en-aut-sei=Isooka
en-aut-mei=Nami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakeshimaMika
en-aut-sei=Takeshima
en-aut-mei=Mika
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SonobeKanau
en-aut-sei=Sonobe
en-aut-mei=Kanau
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AraiRei
en-aut-sei=Arai
en-aut-mei=Rei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=FunakoshiHidemaru
en-aut-sei=Funakoshi
en-aut-mei=Hidemaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=QuinKyle E.
en-aut-sei=Quin
en-aut-mei=Kyle E.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=Smart Smart
en-aut-sei=Smart
en-aut-mei= Smart
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ZenshoKazumasa
en-aut-sei=Zensho
en-aut-mei=Kazumasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=AsanumaMasato
en-aut-sei=Asanuma
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=Bisphenol A diglycidyl ether
kn-keyword=Bisphenol A diglycidyl ether
en-keyword=Epoxy resin
kn-keyword=Epoxy resin
en-keyword=Brain development
kn-keyword=Brain development
en-keyword=Neuronal differentiation
kn-keyword=Neuronal differentiation
en-keyword=Anxiety behavior
kn-keyword=Anxiety behavior
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=2
article-no=
start-page=115
end-page=122
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=202004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Increased Plasma Levels of Platelet Factor 4 and β-thromboglobulin in Women with Recurrent Pregnancy Loss
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Thrombosis in decidual vessels is one of the mechanisms of pregnancy loss. However, few studies have assessed the relation between platelet activation, which is known to cause of thrombosis, and recurrent pregnancy loss (RPL). We investigated platelet activation in women with RPL compared to controls by measuring plasma levels of platelet factor 4 (PF4) and β-thromboglobulin (βTG), and assessed correlations between PF4/βTG and coagulative risk factors associated with RPL. The study group included 135 women who had experienced two or more consecutive pregnancy losses. The control group included 28 age-matched healthy women who had never experienced pregnancy loss. PF4 and βTG plasma levels were significantly higher in the women with RPL than controls (PF4: 14.0 [8.0-20.0] vs. 9.0 [6.0-12.0] ng/ml, p=0.043; βTG: 42.0 [24.3-59.8] vs. 31.5 [26.6-36.4] ng/ml, p=0.002). There was a significant association between βTG and anti-phosphatidylethanolamine antibody immunoglobulin M (aPE IgM) (p=0.048). Among the women with RPL, 18 of those who were positive for PF4 (45%) and 18 of those who were positive for βTG (37%) were negative for all known coagulative risk factors associated with RPL. Measurements of PF4 and βTG may be important because they help identify women who are at risk of RPL.
en-copyright=
kn-copyright=

en-aut-name=KotaniSayoko
en-aut-sei=Kotani
en-aut-mei=Sayoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KamadaYasuhiko
en-aut-sei=Kamada
en-aut-mei=Yasuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ShimizuKeiko
en-aut-sei=Shimizu
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SakamotoAi
en-aut-sei=Sakamoto
en-aut-mei=Ai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NakatsukaMikiya
en-aut-sei=Nakatsuka
en-aut-mei=Mikiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HiramatsuYuji
en-aut-sei=Hiramatsu
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MasuyamaHisashi
en-aut-sei=Masuyama
en-aut-mei=Hisashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Okayama Rosai Hospital
kn-affil=

affil-num=4
en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Okayama City Hospital
kn-affil=

affil-num=7
en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=recurrent pregnancy loss
kn-keyword=recurrent pregnancy loss
en-keyword=platelet factor 4
kn-keyword=platelet factor 4
en-keyword=β-thromboglobulin
kn-keyword=β-thromboglobulin
en-keyword=platelet activation
kn-keyword=platelet activation
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=3
article-no=
start-page=849
end-page=856
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191219
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=YES1 activation induces acquired resistance to neratinib in HER2-amplified breast and lung cancers
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Molecular-targeted therapies directed against human epidermal growth factor receptor 2 (HER2) are evolving for various cancers. Neratinib is an irreversible pan-HER tyrosine kinase inhibitor and has been approved by the FDA as an effective drug for HER2-positive breast cancer. However, acquired resistance of various cancers to molecular-targeted drugs is an issue of clinical concern, and emergence of resistance to neratinib is also considered inevitable. In this study, we established various types of neratinib-resistant cell lines from HER2-amplified breast and lung cancer cell lines using several drug exposure conditions. We analyzed the mechanisms of emergence of the resistance in these cell lines and explored effective strategies to overcome the resistance. Our results revealed that amplification of YES1, which is a member of the SRC family, was amplified in two neratinib-resistant breast cancer cell lines and one lung cancer cell line. Knockdown of YES1 by siRNA and pharmacological inhibition of YES1 by dasatinib restored the sensitivity of the YES1-amplified cell lines to neratinib in vitro. Combined treatment with dasatinib and neratinib inhibited tumor growth in vivo. This combination also induced downregulation of signaling molecules such as HER2, AKT and MAPK. Our current results indicate that YES1 plays an important role in the emergence of resistance to HER2-targeted drugs, and that dasatinib enables such acquired resistance to neratinib to be overcome.
en-copyright=
kn-copyright=

en-aut-name=TakedaTatsuaki
en-aut-sei=Takeda
en-aut-mei=Tatsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamamotoHiromasa
en-aut-sei=Yamamoto
en-aut-mei=Hiromasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SuzawaKen
en-aut-sei=Suzawa
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TomidaShuta
en-aut-sei=Tomida
en-aut-mei=Shuta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MiyauchiShunsaku
en-aut-sei=Miyauchi
en-aut-mei=Shunsaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ArakiKota
en-aut-sei=Araki
en-aut-mei=Kota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NakataKentaro
en-aut-sei=Nakata
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MiuraAkihiro
en-aut-sei=Miura
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NambaKei
en-aut-sei=Namba
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ShienKazuhiko
en-aut-sei=Shien
en-aut-mei=Kazuhiko
kn-aut-name=\
kn-aut-sei=
kn-aut-mei=\
aut-affil-num=10
ORCID=

en-aut-name=SohJunichi
en-aut-sei=Soh
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=ShienTadahiko
en-aut-sei=Shien
en-aut-mei=Tadahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KitamuraYoshihisa
en-aut-sei=Kitamura
en-aut-mei=Yoshihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=SendoToshiaki
en-aut-sei=Sendo
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=ToyookaShinichi
en-aut-sei=Toyooka
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

affil-num=1
en-affil=Department of Clinical Pharmacy, Okayama University Graduate School of Medicine,Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Center for Comprehensive Genomic Medicine, Okayama University Hospital
kn-affil=

affil-num=5
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=13
en-affil=Department of Clinical Pharmacy, Okayama University Graduate School of Medicine,Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=14
en-affil=Department of Clinical Pharmacy, Okayama University Graduate School of Medicine,Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of General Thoracic Surgery and Breast and Endocrinological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=breast cancer
kn-keyword=breast cancer
en-keyword=drug resistance
kn-keyword=drug resistance
en-keyword=lung cancer
kn-keyword=lung cancer
en-keyword=neratinib
kn-keyword=neratinib
en-keyword=YES1
kn-keyword=YES1
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=7
article-no=
start-page=783
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190712
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of Lubrication and Forging Load on Surface Roughness, Residual Stress, and Deformation of Cold Forging Tools
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cold forging is a metal forming that which uses localized compressive force at room temperature. During the cold forging process, the tool is subjected to extremely high loads and abrasive wear. Lubrication plays an important role in cold forging to improve product quality and tool life by preventing direct metallic contact. Surface roughness and residual stress also greatly affects the service life of a tool. In this study, variations in surface roughness, residual stress, and specimen deformation with the number of cold forging cycles were investigated under different forging conditions. Specimens that were made of heat-treated SKH51 (59-61 HRC), a high-speed tool steel with a polished working surface, were used. The specimens were subjected to an upsetting process. Compressive residual stress, surface roughness, and specimen deformation showed a positive relationship with the number of forging cycles up to a certain limit and became almost constant in most of the forging conditions. A larger change in residual stress and surface roughness was observed at the center of the specimens in all the forging conditions. The effect of the magnitude of the forging load on the above discussed parameters is large when compared to the effect of the lubrication conditions.
en-copyright=
kn-copyright=

en-aut-name=KarunathilakaNuwan
en-aut-sei=Karunathilaka
en-aut-mei=Nuwan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TadaNaoya
en-aut-sei=Tada
en-aut-mei=Naoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UemoriTakeshi
en-aut-sei=Uemori
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HanamitsuRyota
en-aut-sei=Hanamitsu
en-aut-mei=Ryota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=FujiiMasahiro
en-aut-sei=Fujii
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OmiyaYuya
en-aut-sei=Omiya
en-aut-mei=Yuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KawanoMasahiro
en-aut-sei=Kawano
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=7
en-affil=Zeno Tech Co., Ltd
kn-affil=
en-keyword=cold forging
kn-keyword=cold forging
en-keyword=high-speed tool steel
kn-keyword=high-speed tool steel
en-keyword=lubrication
kn-keyword=lubrication
en-keyword=residual stress
kn-keyword=residual stress
en-keyword=surface roughness
kn-keyword=surface roughness
en-keyword=tool deformation
kn-keyword=tool deformation
END

start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=5
article-no=
start-page=1042
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190227
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Targeting Ovarian Cancer Cells Overexpressing CD44 with Immunoliposomes Encapsulating Glycosylated Paclitaxel
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Paclitaxel (PTX) is one of the front-line drugs approved for the treatment of ovarian cancer. However, the application of PTX is limited due to the significant hydrophobicity and poor pharmacokinetics. We previously reported target-directed liposomes carrying tumor-selective conjugated antibody and encapsulated glycosylated PTX (gPTX-L) which successfully overcome the PTX limitation. The tubulin stabilizing activity of gPTX was equivalent to that of PTX while the cytotoxic activity of gPTX was reduced. In human ovarian cancer cell lines, SK-OV-3 and OVK18, the concentration at which cell growth was inhibited by 50% (IC50) for gPTX range from 15⁻20 nM, which was sensitive enough to address gPTX-L with tumor-selective antibody coupling for ovarian cancer therapy. The cell membrane receptor CD44 is associated with cancer progression and has been recognized as a cancer stem cell marker including ovarian cancer, becoming a suitable candidate to be targeted by gPTX-L therapy. In this study, gPTX-loading liposomes conjugated with anti-CD44 antibody (gPTX-IL) were assessed for the efficacy of targeting CD44-positive ovarian cancer cells. We successfully encapsulated gPTX into liposomes with the loading efficiency (LE) more than 80% in both of gPTX-L and gPTX-IL with a diameter of approximately 100 nm with efficacy of enhanced cytotoxicity in vitro and of convenient treatment in vivo. As the result, gPTX-IL efficiently suppressed tumor growth in vivo. Therefore gPTX-IL could be a promising formulation for effective ovarian cancer therapies.
en-copyright=
kn-copyright=

en-aut-name=Apriliana Cahya Khayrani
en-aut-sei=Apriliana Cahya Khayrani
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MahmudHafizah
en-aut-sei=Mahmud
en-aut-mei=Hafizah
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ZahraMaram H.
en-aut-sei=Zahra
en-aut-mei=Maram H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Aung Ko Ko Oo
en-aut-sei=Aung Ko Ko Oo
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OzeMiharu
en-aut-sei=Oze
en-aut-mei=Miharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=DuJuan
en-aut-sei=Du
en-aut-mei=Juan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=AlamMd Jahangir
en-aut-sei=Alam
en-aut-mei=Md Jahangir
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=AfifySaid M.
en-aut-sei=Afify
en-aut-mei=Said M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=Hagar A. Abu Quora
en-aut-sei=Hagar A. Abu Quora
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ShigehiroTsukasa
en-aut-sei=Shigehiro
en-aut-mei=Tsukasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=Anna Sanchez Calle
en-aut-sei=Anna Sanchez Calle
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=OkadaNobuhiro
en-aut-sei=Okada
en-aut-mei=Nobuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=SenoAkimasa
en-aut-sei=Seno
en-aut-mei=Akimasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=FujitaKoki
en-aut-sei=Fujita
en-aut-mei=Koki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=HamadaHiroki
en-aut-sei=Hamada
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=SenoYuhki
en-aut-sei=Seno
en-aut-mei=Yuhki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=MandaiTadakatsu
en-aut-sei=Mandai
en-aut-mei=Tadakatsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=SenoMasaharu
en-aut-sei=Seno
en-aut-mei=Masaharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

affil-num=1
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=4
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=5
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=6
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=7
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=8
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=9
en-affil=Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=10
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=11
en-affil= Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
kn-affil=

affil-num=12
en-affil= Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=13
en-affil=Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=14
en-affil=Ensuiko Sugar Refining Co., Ltd.
kn-affil=

affil-num=15
en-affil=Faculty of Science, Okayama University of Science
kn-affil=

affil-num=16
en-affil= Graduate School of Pharmaceutical Science, Tokushima University
kn-affil=

affil-num=17
en-affil= Faculty of Life Science, Kurashiki University of Science and the Arts
kn-affil=

affil-num=18
en-affil= Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=CD44
kn-keyword=CD44
en-keyword=glycosylated paclitaxel
kn-keyword=glycosylated paclitaxel
en-keyword=liposome
kn-keyword=liposome
en-keyword=modified paclitaxel
kn-keyword=modified paclitaxel
en-keyword=ovarian cancer
kn-keyword=ovarian cancer
en-keyword=specific targeting
kn-keyword=specific targeting
END

start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=
article-no=
start-page=100204
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of intravenous immunoglobulin therapy on anti-NT5C1A antibody-positive inclusion body myositis after successful treatment of hepatitis C: A case report
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Inclusion body myositis (IBM) is the commonest idiopathic inflammatory myopathy of older persons. Pathophysiological mechanism of IBM remains unknown; however, an association of IBM with chronic hepatitis C virus (HCV) infection and serum autoantibodies against skeletal muscle protein 5′-nucleotidase 1A (NT5C1A) has recently been reported. No effective treatment for IBM has yet been developed. We here present a 70-year-old man who was anti-NT5C1A antibody-positive in association with IBM and chronic hepatitis C. The initial treatment of ombitasvir/paritaprevir/ritonavir for his chronic hepatitis C was successful; however, his symptoms of IBM did not improve. On the contrary, his quadriplegic paralysis became more severe and he developed dysphagia. Next, steroid pulse therapy was initiated for IBM and, although his hyper-creatine phosphokinase-emia improved, his symptoms did not; indeed, they worsened. Subsequent intravenous immunoglobulin therapy (IVIg) resulted in obvious improvement in his dysphagia. Thereafter IVIg therapy was repeated at approximately 2-monthly intervals. His dysphagia remained improved for more than 1 year; however, his quadriplegia continued to progress slowly. Although IBM can reportedly be associated with hepatitis C, we inferred that there was no direct relationship between these conditions in our patient because his IBM did not improve after treatment of his hepatitis C. Although his IBM-associated quadriplegia did not improve, IVIg therapy did result in improvement in his dysphagia.
en-copyright=
kn-copyright=

en-aut-name=Takamiya Motonori
en-aut-sei=Takamiya
en-aut-mei= Motonori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Takahashi Yoshiaki
en-aut-sei=Takahashi
en-aut-mei= Yoshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Morimoto Mizuki
en-aut-sei=Morimoto
en-aut-mei= Mizuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Morimoto Nobutoshi
en-aut-sei=Morimoto
en-aut-mei= Nobutoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=Yamashita Satoshi
en-aut-sei=Yamashita
en-aut-mei= Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=Abe Koji
en-aut-sei=Abe
en-aut-mei= Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=2
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=3
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=4
en-affil=Department of Neurology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=5
en-affil=Department of Neurology, Graduate School of Medical Sciences, Kumamoto University
kn-affil=

affil-num=6
en-affil=Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
kn-affil=
en-keyword=Anti-skeletal muscle protein 5′-nucleotidase 1A antibody
kn-keyword=Anti-skeletal muscle protein 5′-nucleotidase 1A antibody
en-keyword= Chronic hepatitis C
kn-keyword= Chronic hepatitis C
en-keyword=Dysphagia
kn-keyword=Dysphagia
en-keyword=Inclusion body myositis
kn-keyword=Inclusion body myositis
en-keyword=Intravenous immunoglobulin therapy
kn-keyword=Intravenous immunoglobulin therapy
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=11
article-no=
start-page=2009
end-page=2018
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190730
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Rapid Acquisition of Alectinib Resistance in ALK-Positive Lung Cancer With High Tumor Mutation Burden
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Introduction<br/>
The highly selective ALK receptor tyrosine kinase (ALK) inhibitor alectinib is standard therapy for ALK-positive lung cancers; however, some tumors quickly develop resistance. Here, we investigated the mechanism associated with rapid acquisition of resistance using clinical samples. <br/>
Methods<br/>
Autopsied samples were obtained from lung, liver, and renal tumors from a 51-year-old male patient with advanced ALK-positive lung cancer who had acquired resistance to alectinib in only 3 months. We established an alectinib-resistant cell line (ABC-14) from pleural effusion and an alectinib/crizotinib-resistant cell line (ABC-17) and patient-derived xenograft (PDX) model from liver tumors. Additionally, we performed next-generation sequencing, direct DNA sequencing, and quantitative real-time reverse transcription polymerase chain reaction. <br/>Results<br/>
ABC-14 cells harbored no ALK mutations and were sensitive to crizotinib while also exhibiting MNNG HOS transforming gene (MET) gene amplification and amphiregulin overexpression. Additionally, combined treatment with crizotinib/erlotinib inhibited cell growth. ABC-17 and PDX tumors harbored ALK G1202R, and PDX tumors metastasized to multiple organs in vivo, whereas the third-generation ALK-inhibitor, lorlatinib, diminished tumor growth in vitro and in vivo. Next-generation sequencing indicated high tumor mutation burden and heterogeneous tumor evolution. The autopsied lung tumors harbored ALK G1202R (c. 3604 G>A) and the right renal metastasis harbored ALK G1202R (c. 3604 G>C); the mutation thus comprised different codon changes. <br/>Conclusions<br/>
High tumor mutation burden and heterogeneous tumor evolution might be responsible for rapid acquisition of alectinib resistance. Timely lorlatinib administration or combined therapy with an ALK inhibitor and other receptor tyrosine-kinase inhibitors might constitute a potent strategy.
en-copyright=
kn-copyright=

en-aut-name=MakimotoGo
en-aut-sei=Makimoto
en-aut-mei=Go
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OhashiKadoaki
en-aut-sei=Ohashi
en-aut-mei=Kadoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TomidaShuta
en-aut-sei=Tomida
en-aut-mei=Shuta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NishiiKazuya
en-aut-sei=Nishii
en-aut-mei=Kazuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MatsubaraTakehiro
en-aut-sei=Matsubara
en-aut-mei=Takehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KayataniHiroe
en-aut-sei=Kayatani
en-aut-mei=Hiroe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HigoHisao
en-aut-sei=Higo
en-aut-mei=Hisao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NinomiyaKiichiro
en-aut-sei=Ninomiya
en-aut-mei=Kiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SatoAkiko
en-aut-sei=Sato
en-aut-mei=Akiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=WatanabeHiromi
en-aut-sei=Watanabe
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KanoHirohisa
en-aut-sei=Kano
en-aut-mei=Hirohisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=NinomiyaTakashi
en-aut-sei=Ninomiya
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KuboToshio
en-aut-sei=Kubo
en-aut-mei=Toshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=RaiKammei
en-aut-sei=Rai
en-aut-mei=Kammei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=IchiharaEiki
en-aut-sei=Ichihara
en-aut-mei=Eiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=HottaKatsuyuki
en-aut-sei=Hotta
en-aut-mei=Katsuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=TabataMasahiro
en-aut-sei=Tabata
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=ToyookaShinichi
en-aut-sei=Toyooka
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=TakataMinoru
en-aut-sei=Takata
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=MaedaYoshinobu
en-aut-sei=Maeda
en-aut-mei=Yoshinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=KiuraKatsuyuki
en-aut-sei=Kiura
en-aut-mei=Katsuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

affil-num=1
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Okayama University Hospital Biobank, Okayama University Hospital
kn-affil=

affil-num=4
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Okayama University Hospital Biobank, Okayama University Hospital
kn-affil=

affil-num=6
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=10
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=12
en-affil=Department of Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=13
en-affil=Center for Clinical Oncology, Okayama University Hospital
kn-affil=

affil-num=14
en-affil=Department of Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=15
en-affil=Department of Respiratory Medicine, Okayama University Hospital
kn-affil=

affil-num=16
en-affil=Center of Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=17
en-affil=Center for Clinical Oncology, Okayama University Hospital
kn-affil=

affil-num=18
en-affil=Okayama University Hospital Biobank, Okayama University Hospital
kn-affil=

affil-num=19
en-affil=Laboratory of DNA Damage Signaling, Department of Late Effects Studies, Graduate School of Biostudies, Radiation Biology Center, Kyoto University
kn-affil=

affil-num=20
en-affil=Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=21
en-affil=Department of Respiratory Medicine, Okayama University Hospital
kn-affil=
en-keyword=ALK G1202R
kn-keyword=ALK G1202R
en-keyword=Alectinib
kn-keyword=Alectinib
en-keyword=Amphiregulin
kn-keyword=Amphiregulin
en-keyword=MET
kn-keyword=MET
en-keyword=NSCLC
kn-keyword=NSCLC
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=4
article-no=
start-page=285
end-page=297
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=201908
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Dynamic Reorganization of Microtubule and Glioma Invasion
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Gliomas are characterized as highly diffuse infiltrating tumors, and currently available treatments such as surgery, radiation and chemotherapy are unfeasible or show limited efficacy against these tumors. Recent genetic and epigenetic analyses of glioma have revealed increasing evidence of the role of driver genetic alterations in glioma development and led to the identification of prognostic factors. Despite these findings, the survival rates of glioma patients remain low, and alternative treatments and novel targets are needed. Recent studies identified neural stem cells as the possible origin of gliomas, and some evidence has revealed shared functions and mechanisms between glioma cells and neurons, also supporting their similarity. The cytoskeleton plays important roles in the migration of normal cells as well as cancer cells. Recent reports have described a role for microtubules, a component of the cytoskeleton, in glioma invasion. Notably, several factors that regulate microtubule functions, such as microtubule-associated proteins, plus-end tracking proteins, or motor proteins, are upregulated in glioma tissues compared with normal tissue, and upregulation of these factors is associated with high invasiveness of glioma cells. In this review, we describe the mechanism of microtubules in glioma invasion and discuss the possibility of microtubule-targeted therapy to inhibit glioma invasion.
en-copyright=
kn-copyright=

en-aut-name=OtaniYoshihiro
en-aut-sei=Otani
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IchikawaTomotsugu
en-aut-sei=Ichikawa
en-aut-mei=Tomotsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurozumiKazuhiko
en-aut-sei=Kurozumi
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=DateIsao
en-aut-sei=Date
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Neurosurgery, The University of Texas Health Science Center at Houston
kn-affil=

affil-num=2
en-affil=Department of Neurosurgery, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=3
en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=glioma
kn-keyword=glioma
en-keyword=cytoskeletons
kn-keyword=cytoskeletons
en-keyword=invasion
kn-keyword=invasion
en-keyword=microtubules
kn-keyword=microtubules
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=2
article-no=
start-page=117
end-page=125
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=201904
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Unintentional Injury Deaths among Children: A Descriptive Study Using Medico-legal Documents in Okayama Prefecture, Japan (2001−2015)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= According to the World Health Organization’s World Report, approx. 950,000 children and young people < 18 years old die from an injury each year, and unintentional injury deaths account for a large portion of these cases. Here we used medico-legal documents to epidemiologically analyze the cases of unintentional injury deaths among children < 5 years old in Okayama Prefecture, Japan from 2001 to 2015. Age, sex, manner/cause of death, and various circumstances of the incident were investigated. There were 73 unintentional injury deaths during the study period. Drowning (n=29), suffocation (n=24), and transport accidents (n=13) were the major categories of unintentional injury deaths. Twenty-two cases (30.1%) were autopsied. Differences in the characteristics of the unintentional injury deaths by age were observed. Information which cannot be obtained from Vital Statistics was available from medico-legal documents, and detailed characteristics of unintentional injury deaths among children < 5 years old were elucidated. Investigating medico-legal information is one of the meaningful measures for the prevention of unintentional injury deaths among children in Japan.
en-copyright=
kn-copyright=

en-aut-name=YamasakiYukie
en-aut-sei=Yamasaki
en-aut-mei=Yukie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TamiyaNanako
en-aut-sei=Tamiya
en-aut-mei=Nanako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamamotoHideki
en-aut-sei=Yamamoto
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MiyaishiSatoru
en-aut-sei=Miyaishi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Health Services Research, Faculty of Medicine, University of Tsukuba,
kn-affil=

affil-num=3
en-affil=Graduate School of Public Health, Teikyo University
kn-affil=

affil-num=4
en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=child death
kn-keyword=child death
en-keyword=unintentional injury
kn-keyword=unintentional injury
en-keyword=prevention
kn-keyword=prevention
en-keyword=medico-legal document
kn-keyword=medico-legal document
END

start-ver=1.4
cd-journal=joma
no-vol=37
cd-vols=
no-issue=6
article-no=
start-page=1258
end-page=1266
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=201512
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Exploring autistic-like traits relating to empathic attitude and psychological distress in hospital pharmacists
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=BACKGROUND:
Pharmacists are expected to play a key role in modern cancer care. Research suggests that an empathic approach and attitude in medical staff improves the quality of patient care. An empathic attitude and psychological distress are thought to be associated with autistic-like traits, but little is known about such traits.
OBJECTIVE:
In this study, we aimed to clarify the associations among autistic-like traits, empathic attitude in a medical context, and psychological health in hospital pharmacists.
SETTING:
Eligibility criteria for inclusion were certified pharmacists working at hospitals for patient care who returned their questionnaires.
METHOD:
Eight hundred and twenty-three hospital pharmacists completed a number of self-administered questionnaires anonymously by mail.
MAIN OUTCOME MEASURES:
Scores were obtained on the Autism-Spectrum Quotient, the Jefferson Scale of Empathy, the General Health Questionnaire-12, and subscales of the Interpersonal Reactivity Index (Perspective Taking, IRI-Empathic Concern, IRIPersonal Distress). We performed correlation and mediation analyses to confirm that the empathy and general health questionnaires were associated with autism-spectrum quotient scores, and with each IRI subscale.
RESULTS:
Complete responses were obtained from 379 pharmacists comprising 151 males (39.8 %) with a mean age of 37.7 ± 10.8 years (missing data, n = 13) and a median of 11 years after qualification as a pharmacist. Autism-Spectrum Quotient scores were inversely correlated with empathy (r = -0.22, p < 0.001) and positively correlated with general health scores (r = 0.40, p < 0.001). In the models with mediation, the inverse correlation between autism-spectrum quotient and empathy scores was mediated indirectly by IRI-Perspective Taking and IRI-Empathic Concern, and the positive correlation between autism-spectrum quotient and general health was mediated indirectly by IRI-Personal Distress. There were also direct effects, with significant effects of autism-spectrum quotient on empathy and general health scores.
CONCLUSION:
Our findings suggest that autistic-like traits affect both empathic attitude in a medical context and the psychological health of pharmacists. We recommend that to improve empathy in those with high levels of autistic-like traits, we may need to develop specialized interventions, such as improving communication skills training.
en-copyright=
kn-copyright=

en-aut-name=HiguchiYuji 
en-aut-sei=Higuchi
en-aut-mei=Yuji 
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UchitomiYosuke
en-aut-sei=Uchitomi
en-aut-mei=Yosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FujimoriMaiko
en-aut-sei=Fujimori
en-aut-mei=Maiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KoyamaToshihiro
en-aut-sei=Koyama
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KataokaHitomi
en-aut-sei=Kataoka
en-aut-mei=Hitomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KitamuraYoshihisa
en-aut-sei=Kitamura
en-aut-mei=Yoshihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SendoToshiaki
en-aut-sei=Sendo
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=InagakiMasatoshi
en-aut-sei=Inagaki
en-aut-mei=Masatoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of NeuropsychiatryOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Innovation Center for Supportive, Palliative and Psychosocial CareNational Cancer Center
kn-affil=

affil-num=3
en-affil=Center for Suicide Prevention, National Institute of Mental HealthNational Center for Neurology and Psychiatry
kn-affil=

affil-num=4
en-affil=Department of Clinical PharmacyOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Primary Care and Medical EducationOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Clinical PharmacyOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Clinical PharmacyOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of NeuropsychiatryOkayama University Hospital
kn-affil=
en-keyword=Empathy
kn-keyword=Empathy
en-keyword= Hospital pharmacist
kn-keyword= Hospital pharmacist
en-keyword= Japan
kn-keyword= Japan
en-keyword= Pharmaceutical care 
kn-keyword= Pharmaceutical care 
END

start-ver=1.4
cd-journal=joma
no-vol=127
cd-vols=
no-issue=3
article-no=
start-page=213
end-page=218
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20151201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A case report of giant ectopic pheochromocytoma conversion therapy with radioisotope therapy and chemotherapy followed by curative resection
kn-title=術前内照射および化学療法が著効し,根治切除し得た巨大異所性褐色細胞腫の1例
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A 46-year-old man was found to be positive for occult blood at a medical checkup and was revealed to have a 14-cm tumor on the right side of abdominal aorta by a subsequent abdominal CT scan. The endocrinology laboratory data showed elevations in the levels of serum noradrenaline, and ectopic pheochromocytoma was suspected.
 The tumor was compressing the inferior vena cava and portal vein, the superior mesenteric artery and the pancreas. Since it would be difficult to cure by operation, neoadjuvant therapy was started using radioisotope therapy by I-131 metaiodobenzylguanidine (131I-MIBG) and chemotherapy (CVD therapy ; cyclophosphamide, vincristine, dacarbazine). He was treated with three courses of radioisotope therapy and 16 courses of chemotherapy, which significantly reduced the tumor size. This made radical resection possible ; we were able to avoid the merger excision of great vessels and other organs.
 On pathological and immunopathological findings, the tumor was diagnosed as ectopic　pheochromocytoma. Regarding the safety and curability of the treatment, neoadjuvant therapy may be useful in treating very large tumors that show invasion of other organs.
en-copyright=
kn-copyright=

en-aut-name=YasuiKazuya
en-aut-sei=Yasui
en-aut-mei=Kazuya
kn-aut-name=安井和也
kn-aut-sei=安井
kn-aut-mei=和也
aut-affil-num=1
ORCID=

en-aut-name=UmedaYuzo
en-aut-sei=Umeda
en-aut-mei=Yuzo
kn-aut-name=楳田祐三
kn-aut-sei=楳田
kn-aut-mei=祐三
aut-affil-num=2
ORCID=

en-aut-name=KumanoKenjiro
en-aut-sei=Kumano
en-aut-mei=Kenjiro
kn-aut-name=熊野健二郎
kn-aut-sei=熊野
kn-aut-mei=健二郎
aut-affil-num=3
ORCID=

en-aut-name=TabataMasahiro
en-aut-sei=Tabata
en-aut-mei=Masahiro
kn-aut-name=田端雅弘
kn-aut-sei=田端
kn-aut-mei=雅弘
aut-affil-num=4
ORCID=

en-aut-name=OtsukaFumio
en-aut-sei=Otsuka
en-aut-mei=Fumio
kn-aut-name=大塚文男
kn-aut-sei=大塚
kn-aut-mei=文男
aut-affil-num=5
ORCID=

en-aut-name=YagiTakahito
en-aut-sei=Yagi
en-aut-mei=Takahito
kn-aut-name=八木孝仁
kn-aut-sei=八木
kn-aut-mei=孝仁
aut-affil-num=6
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=藤原俊義
kn-aut-sei=藤原
kn-aut-mei=俊義
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科

affil-num=5
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科

affil-num=6
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科

affil-num=7
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科
en-keyword=異所性褐色細胞腫（ectopic pheochromocytoma）
kn-keyword=異所性褐色細胞腫（ectopic pheochromocytoma）
en-keyword=化学療法（chemo therapy）
kn-keyword=化学療法（chemo therapy）
en-keyword=131I-MIBG
kn-keyword=131I-MIBG
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=1
article-no=
start-page=51
end-page=58
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=201502
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Trend of Human Papillomavirus Genotypes in Cervical Neoplasia Observed in a Newly Developing Township in Yangon, Myanmar
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Persistent infection with oncogenic types of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer. This study detected the oncogenic HPV genotypes in cervical neoplasia in relation to clinicopathological findings using a cross-sectional descriptive method in 2011 and 2012. Cervical swabs and colposcopy-directed cervical biopsy tissues were collected from 108 women (median age 45 years;range 20-78) showing cervical cytological changes at Sanpya General Hospital, Yangon, Myanmar. HPV DNA testing and genotyping were performed by polymerase chain reaction and restriction fragment length polymorphism. HPV was identified in women with cervical intraepithelial neoplasia (CIN) 1 (44.4%), CIN2 (63.2%), CIN3 (70.6%), and squamous cell carcinoma (SCC) (74.1%). The association between cervical neoplasia and HPV positivity was highly significant (p＝0.008). Most patients infected with HPV were between 40-49 years of age, and the youngest were in the 20- to 29-year-old age group. The most common genotype was HPV 16 (65.6%) with the following distribution:70% in CIN1, 41.7% in CIN2, 91.7% in CIN3, and 60% in SCC. HPV-31 was the second-most frequent (21.9%):30% in CIN1, 33.3% in CIN2, 8.3% in CIN3, and 15% in SCC. The third-most frequent-genotype was HPV-18 (7.8%):8.3% in CIN1, and 20% in SCC. Another genotype was HPV-58 (4.7%):16.7% in CIN1 and 5% in SCC. The majority of CIN/SCC cases were associated with HPV genotypes 16, 31, 18, and 58. If oncogenic HPV genotypes are positive, the possibility of cervical neoplasia can be predicted. Knowledge of the HPV genotypes distribution can predict the effectiveness of the currently used HPV vaccine.
en-copyright=
kn-copyright=

en-aut-name=Mu Mu Shwe
en-aut-sei=Mu Mu Shwe
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Kyi Kyi Nyunt
en-aut-sei=Kyi Kyi Nyunt
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OkadaShigeru
en-aut-sei=Okada
en-aut-mei=Shigeru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HaranoTeruo
en-aut-sei=Harano
en-aut-mei=Teruo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=Hlaing Myat Thu
en-aut-sei=Hlaing Myat Thu
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=Hla Myat Mo Mo
en-aut-sei=Hla Myat Mo Mo
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=Mo Mo Win
en-aut-sei=Mo Mo Win
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=Khin Khin Oo
en-aut-sei=Khin Khin Oo
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KhinThet Wai
en-aut-sei=KhinThet Wai
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=Khin Saw Aye
en-aut-sei=Khin Saw Aye
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=Myo Khin
en-aut-sei=Myo Khin
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Medical Research (Lower Myanmar), Minstry of Health

affil-num=2
en-affil=
kn-affil=Sanpya General Hospital

affil-num=3
en-affil=
kn-affil=Professor Emeritus, Okayama University

affil-num=4
en-affil=
kn-affil=Department of General Medicine, Okayama University Hospital

affil-num=5
en-affil=
kn-affil=Department of Medical Research (Lower Myanmar), Minstry of Health

affil-num=6
en-affil=
kn-affil=Sanpya General Hospital

affil-num=7
en-affil=
kn-affil=Department of Medical Research (Lower Myanmar), Minstry of Health

affil-num=8
en-affil=
kn-affil=Department of Medical Research (Lower Myanmar), Minstry of Health

affil-num=9
en-affil=
kn-affil=Department of Medical Research (Lower Myanmar), Minstry of Health

affil-num=10
en-affil=
kn-affil=Department of Medical Research (Lower Myanmar), Minstry of Health

affil-num=11
en-affil=
kn-affil=Department of Medical Research (Lower Myanmar), Minstry of Health
en-keyword=human papillomavirus
kn-keyword=human papillomavirus
en-keyword=genotyping
kn-keyword=genotyping
en-keyword=Myanmar
kn-keyword=Myanmar
END

start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20141120
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Nuclear factor-kappaB sensitizes to benzyl isothiocyanate-induced antiproliferation in p53-deficient colorectal cancer cells
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Benzyl isothiocyanate (BITC), a dietary isothiocyanate derived from cruciferous vegetables, inhibits the proliferation of colorectal cancer cells, most of which overexpress β-catenin as a result of mutations in the genes for adenomatous polyposis coli or mutations in β-catenin itself. Because nuclear factor-κB (NF-κB) is a plausible target of BITC signaling in inflammatory cell models, we hypothesized that it is also involved in BITC-inhibited proliferation of colorectal cancer cells. siRNA-mediated knockdown of the NF-κB p65 subunit significantly decreased the BITC sensitivity of human colorectal cancer HT-29 cells with mutated p53 tumor suppressor protein. Treating HT-29 cells with BITC induced the phosphorylation of IκB kinase, IκB-α and p65, the degradation of IκB-α, the translocation of p65 to the nucleus and the upregulation of NF-κB transcriptional activity. BITC also decreased β-catenin binding to a positive cis element of the cyclin D1 promoter and thus inhibited β-catenin-dependent cyclin D1 transcription, possibly through a direct interaction between p65 and β-catenin. siRNA-mediated knockdown of p65 confirmed that p65 negatively affects cyclin D1 expression. On the other hand, when human colorectal cancer HCT-116 cells with wild-type p53 were treated with BITC, translocation of p65 to the nucleus was inhibited rather than enhanced. p53 knockout increased the BITC sensitivity of HCT-116 cells in a p65-dependent manner, suggesting that p53 negatively regulates p65-dependent effects. Together, these results identify BITC as a novel type of antiproliferative agent that regulates the NF-κB pathway in p53-deficient colorectal cancer cells.
en-copyright=
kn-copyright=

en-aut-name=AbeN
en-aut-sei=Abe
en-aut-mei=N
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HouD-X
en-aut-sei=Hou
en-aut-mei=D-X
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MunemasaS
en-aut-sei=Munemasa
en-aut-mei=S
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MurataY
en-aut-sei=Murata
en-aut-mei=Y
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NakamuraY
en-aut-sei=Nakamura
en-aut-mei=Y
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University

affil-num=2
en-affil=
kn-affil=Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

affil-num=3
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University

affil-num=4
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University

affil-num=5
en-affil=
kn-affil=Graduate School of Environmental and Life Science, Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=124
cd-vols=
no-issue=3
article-no=
start-page=231
end-page=238
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20121203
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=HuH-7 cell line established from a highly differentiated human hepatocellular carcinoma
kn-title=高分化型ヒト肝癌由来細胞株“HuH-7”
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=高分化型ヒト肝癌由来細胞株“HuH-7”は，1982年にCancer Researchにその樹立を報告した．HuH-7は，当時の岡山大学医学部附属癌源研究施設病理部門（故佐藤二郎教授）の下で樹立し，これまで多くの研究分野で利用され，世界的に有名な肝癌細胞株となっている．本稿では，有用性の高い分化機能を有するヒト肝癌細胞株HuH-7について，肝細胞癌の腫瘍マーカーであるα-fetoprotein（AFP）を中心に，この細胞株を用いた研究分野に関する詳細を紹介する．
en-copyright=
kn-copyright=

en-aut-name=NakabayashiHidekazu
en-aut-sei=Nakabayashi
en-aut-mei=Hidekazu
kn-aut-name=中林秀和
kn-aut-sei=中林
kn-aut-mei=秀和
aut-affil-num=1
ORCID=

en-aut-name=TaketaKazuhisa
en-aut-sei=Taketa
en-aut-mei=Kazuhisa
kn-aut-name=武田和久
kn-aut-sei=武田
kn-aut-mei=和久
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=北海道情報大学　医療情報学科

affil-num=2
en-affil=
kn-affil=介護老人保健施設　仁和の里
en-keyword=肝細胞癌
kn-keyword=肝細胞癌
en-keyword=培養細胞
kn-keyword=培養細胞
en-keyword=α-フェトプロテイン
kn-keyword=α-フェトプロテイン
en-keyword=HuH-7
kn-keyword=HuH-7
END

start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=9-10
article-no=
start-page=1311
end-page=1330
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=2011
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Miniature Pneumatic Curling Rubber Actuator Generating Bidirectional Motion with One Air-Supply Tube
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Soft actuators driven by pneumatic pressure are promising actuators for mechanical systems in medical, biological, agriculture, welfare fields and so on, because they can ensure high safety for fragile objects from their low mechanical impedance. In this study, a new rubber pneumatic actuator made from silicone rubber was developed. Composed of one chamber and one air-supply tube, it can generate curling motion in two directions by using positive and negative pneumatic pressure. The rubber actuator, for generating bidirectional motion, was designed to achieve an efficient shape by nonlinear finite element method analysis, and was fabricated by a molding and rubber bonding process using excimer light. The fabricated actuator was able to generate curling motion in two directions successfully. The displacement and force characteristics of the actuator were measured by using a motion capture system and a load cell. As an example application of the actuator, a robotic soft hand with three actuators was constructed and its effectiveness was confirmed by experiments.
en-copyright=
kn-copyright=

en-aut-name=WakimotoShuichi
en-aut-sei=Wakimoto
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SuzumoriKoichi
en-aut-sei=Suzumori
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OguraKeiko
en-aut-sei=Ogura
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
en-keyword=Soft actuator
kn-keyword=Soft actuator
en-keyword=pneumatic actuator
kn-keyword=pneumatic actuator
en-keyword=bidirectional motion
kn-keyword=bidirectional motion
en-keyword=nonlinear analysis
kn-keyword=nonlinear analysis
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-2
article-no=
start-page=7783
end-page=7802
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Oxidation-Reduction Potential and the Bilirubin Deposition of Skin in Jaundiced Patients Part I Studies on the Oxidation-Reduction Potential of Skin in Jaundiced Patients
kn-title=黄疸患者の皮膚の酸化還元電位とbilirubinの沈着に関する研究 第1編 黄疸患者の皮膚の酸化還元電位に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to clarify the condition of oxidation-reduction potential in the skin of jaundiced patients, the author made the preliminary experiment by the injection of 2. 3. 5. triphenyl tetrazolium chloride, neo tetrazolium chloride and blue tetrazolium into dogs and rabbits, employing the Arakawa's method, and it was recognized the T. T. C. test by Arakawa was the best method to apply to human body. Therefore, the test was attempted on 20 cases of healthy human, 110 cases of various liver and bile ducts diseases (75 cases of them showed hyperbilirubinemia over 1 mg/dl) and 21 cases of other diseases, and the above results was compared with various clinical symptoms and clinical courses. And the results were as follows. 1. Using the above agents as a oxidation-reduction. dicator of skin in living body, the sensitivity was in order of N. T C., T. T. C. and B. T., but the side effect was the most scanty in T. T. C., Therefore, the T. T. C. test by Arakawa was the best for the application to human body, and N. T. C. had the utility value on the animal experiment since the stability and sensitivity to light, but B. T. could not use. 2. The difference of oxidation-reduction potential among healty human, dog and rabbit was clearly observed, and this test was the most in rabbit, and then in dog, and the most low in human. 3. The T. T. C. test was almost negative in healthy human, but in was positive in all cases of mechanical jaundice, and it showed positive by the stadium or symptom in acute hepatitis and livercirrhosis, and it was seen at the early period of acute stadium in the former and it was seen in the cases of the latter with continuous jaundice over 60 days. In and it was negative or slight positive in chronic hepatitis. It was negative in hemolytic jaundice and others. 4. Comparing T. T. C. test with various clinical symptoms and various clinical tests, it had relation to serum total bilirubin especially direct bilirubin, alkaline phosphatase and skin itching, and it had considerable relation to serum colloidal reaction in acute hepatitis and BSP-test. 5. Therefore, it was thought that there were close correlation between the oxidationreduction potential of skin and the mechanism in which Verdinikterus was occured from the change of disposed bilirubin in skin to biliverdin.
en-copyright=
kn-copyright=

en-aut-name=IshimitsuTetsusaburo
en-aut-sei=Ishimitsu
en-aut-mei=Tetsusaburo
kn-aut-name=石光鉄三郎
kn-aut-sei=石光
kn-aut-mei=鉄三郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7179
end-page=7184
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Interface Activating Reagents on the Diazo Reaction of Bilirubin Part 1. Effect of Interface Activating Reagents on Bilirubin
kn-title=Bilirubinのdiazo反応に及ぼす界面活性剤の影響 第2篇 Bilirubin dimethylesterへの影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Selecting solvent that dissolves interface activating reagents and bilirubin dimethylester relatively well and dissolving each of them the author prepared an interface active bilirubin-dimethylester aqueous solution. Then evaqorating this solution at 80°C under a lolw pressure, the auther studied the chemical proqerties of the vaqor dissolved in water and compare with the proqerties of bilirubin. The following are the results of this comparative study. 1. Each interface activating reagent possesses the specific capacity to dissolve bilirubin-dimethlyester in water, and there is a direct-proportion relationship between the quantity of bilirubin dimethylester dissolved in water and quantiy of activating reagent required for the dissolution. The bilirubindimethylester dissolving capacity of each reagent is 1.5 to 4.0 times the bilirubin dissolving capacity. 2. The absorption curve of the bilirubin-dimethylester aquoeus solution has its maximum at 420 mμ, and with the increase in the quantity of the activation reagent added, the maximum absorption curve moves 5-10 mμ towards the long side of short wave. However, in the case of non-ionic activating reagent hardly any such change occurs. 3. The bilirubin-dimethylester aqueous solution is always positive to the direct diazo reaction, and its sensitivity to diazo is not affected by the quantity of activating reagent but is fixed. 4. About 50-75 per cent diazo reaction of the bilirubidimethylester aqueous solution is completed within 5 minutes, but it requires about 40 minutes for its completion. 5. Spectroscopically there is no great difference between bilirubin-dimethylester azo and bilirubin azo, but the optical desnity at 490 mμ on the long side of short wave is higher in the former.
en-copyright=
kn-copyright=

en-aut-name=InouéToshio
en-aut-sei=Inoué
en-aut-mei=Toshio
kn-aut-name=井上敏雄
kn-aut-sei=井上
kn-aut-mei=敏雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7171
end-page=7177
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Interface Activating Reagents on the Diazo Reaction of Bilirubin Part 1. Effect of Interface Activating Reagents on Bilirubin
kn-title=Bilirubinのdiazo反応に及ぼす界面活性剤の影響 第1篇 Bilirubinへの影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By selecting the solvent that dissolves interface activating reagents and bilirubin relatively well and after dissolving each the author prepared an interface activ bilirubin aqueous solution. This solution was evaporated at 80°C under a low pressure and the solubility in water and the chemical properties of the vapor were studied; and the following ressults were obtained. 1. Each interface activation reagent possesses its own specific capacity to dissolve bilirubin in water, and there is almost a direct-proportion relation between the quantity of bilirubin dissolved in water and the quantity of activating reagent required for such a dissolution. 2. The absorption curve of the bilirubin aqueous solution shows its maximum at 450 mμ, and with the increase in the quantity of the loaded activating reagent the absorption maximum moves 5-10 mμ towards the long side of the short wave; but in the case using non-ionic reagent the absorption maximum remains almost unchanged. 3. The bilirubin aqueous solution is positive to the direct diazo reaction, and its sensitivity to the diazo reaction is proportional to the quantity of the activating reagent added, making the maximum sensitvity to the diazo reaction stable beyond a given quantity. Therefore, the dissolution of bilirubin in water by the aid of the interface activating reagent and the positivity to the diazo reaction are two different phenomena. Thus it is impossible to say that the dissolution of bilirubin in water make it positive to the direct diazo reaction. 4. The bilirubin aqueous solution by the aid of interface activating reagent required 40 to 60 minutes for the completion of diazo reaction and the production of azo dye is about directly proportional to the time required for its production. 5. The absorption maximum of azo dye produced the diazo reaction always at 530 mμ even when pH of the solvent is changed, and the optical density increases proportionately to the strength of acidity. At the same pH the optical density at 530 mμ of azo dye produced by ionic activating reagent is higher than that of the azo dye produced by non-ionic activating reagent; whereas the optical density of 470 mμ on the long side of short wave, on the contrary, of the azo dye produced by non-ionic activating reagent is higher.
en-copyright=
kn-copyright=

en-aut-name=InouéToshio
en-aut-sei=Inoué
en-aut-mei=Toshio
kn-aut-name=井上敏雄
kn-aut-sei=井上
kn-aut-mei=敏雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=10-1
article-no=
start-page=6223
end-page=6230
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Pharmacological Studies on Dimethoxyisoquinolin Derivatives
kn-title=Dimethoxyisoquinoline誘導体についての二，三薬理学的検索
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Twelve members of a new series of 1-trialkylmethyl-1-6, 7-dimethoxy-3, 4-dihydroisoquinoline (D type) and eleven analogs of 1-trialkylmethyl-6, 7-dimethoxy isoquinoline (I type) were investigated for their pharmacological properties, with special reference to the toxicity and spasmolytic activity, comparing with papaverine. Along these isoquinoline, 1-methylisoquinoline, 6, 7-dimethoxyisoquinoline and their respective 3, 4-hydrogenated derivatives, were also examined for the elucidation of structure-activity relationship. All the compounds of D type produced a marked inhibitory action on the isolated rabbit intestine. The most potent members were n-tripropyl, ethyl-n-propyl-n-butyl and methyl-ethyl-n-butyl derivatives. Their spasmolytic actions on both barium and histamine contraction of the intestine exceeded those of papaverine, though the effect on acetylcholine spasm was slightly inferior. They were less toxic in mice than in the latter. The intestine inhibitory activity and toxicity of I Type compounds were both smaller than those of D type analogs. The differences between the two types seemed to be related to the solubility. Other smaller components of the papaverine structure, namely, the primitive isoquinoline derivatives were more toxic, but much less spasmolytic than papaverine. In mice toxic doses of all the compounds caused tremors, excitability and convulsions followed by a generalized central depression. Respiratory paralysis may be direct cause of death. Toxic symptoms were generally similar to those in frogs. The stimulant properties were increased by the saturation of the 3, 4-position on the isoquinoline nucleus.
en-copyright=
kn-copyright=

en-aut-name=KurodaAkio
en-aut-sei=Kuroda
en-aut-mei=Akio
kn-aut-name=黒田章夫
kn-aut-sei=黒田
kn-aut-mei=章夫
aut-affil-num=1
ORCID=

en-aut-name=JinzenjiKei
en-aut-sei=Jinzenji
en-aut-mei=Kei
kn-aut-name=秦泉寺圭
kn-aut-sei=秦泉寺
kn-aut-mei=圭
aut-affil-num=2
ORCID=

en-aut-name=YamamotoTakashi
en-aut-sei=Yamamoto
en-aut-mei=Takashi
kn-aut-name=山本孝
kn-aut-sei=山本
kn-aut-mei=孝
aut-affil-num=3
ORCID=

en-aut-name=KondoKazuji
en-aut-sei=Kondo
en-aut-mei=Kazuji
kn-aut-name=近藤和二
kn-aut-sei=近藤
kn-aut-mei=和二
aut-affil-num=4
ORCID=

en-aut-name=ShodaJunzo
en-aut-sei=Shoda
en-aut-mei=Junzo
kn-aut-name=正田順蔵
kn-aut-sei=正田
kn-aut-mei=順蔵
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部薬理学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部薬理学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部薬理学教室

affil-num=4
en-affil=
kn-affil=岡山大学医学部薬理学教室

affil-num=5
en-affil=
kn-affil=岡山大学医学部薬理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=2
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2006
dt-pub=20068
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Thermal fluctuations of vortex clusters in quasi-two-dimensional bose-einstein condensates
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We study the thermal fluctuations of vortex positions in small vortex clusters in a harmonically trapped rotating Bose-Einstein condensate. It is shown that the order-disorder transition of two-shell clusters occurs via the decoupling of shells with respect to each other. The corresponding "melting" temperature depends strongly on the commensurability between numbers of vortices in shells. We show that melting can be achieved at experimentally attainable parameters and very low temperatures. Also studied is the effect of thermal fluctuations on vortices in an anisotropic trap with small quadrupole deformation. We show that thermal fluctuations lead to the decoupling of a vortex cluster from the pinning potential produced by this deformation. The decoupling temperatures are estimated and strong commensurability effects are revealed.</p>
en-copyright=
kn-copyright=

en-aut-name=PogosovW V
en-aut-sei=Pogosov
en-aut-mei=W V
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MachidaK
en-aut-sei=Machida
en-aut-mei=K
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University
en-keyword=scissors mode
kn-keyword=scissors mode
en-keyword=gas
kn-keyword=gas
en-keyword=superfluidity
kn-keyword=superfluidity
en-keyword=transition
kn-keyword=transition
en-keyword=stability
kn-keyword=stability
en-keyword=phase
kn-keyword=phase
END

start-ver=1.4
cd-journal=joma
no-vol=123
cd-vols=
no-issue=11
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20059
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Infrared spectra of seeded hydrogen clusters: (para-H2)N-N2O and (ortho-H2)N-N2O, N=2-13
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>High-resolution infrared spectra of clusters containing para-H2 and/or ortho-H2 and a single nitrous oxide molecule are studied in the 2225-cm–1 region of the 1 fundamental band of N2O. The clusters are formed in pulsed supersonic jet expansions from a cooled nozzle and probed using a tunable infrared diode laser spectrometer. The simple symmetric rotor-type spectra generally show no resolved K structure, with prominent Q-branch features for ortho-H2 but not para-H2 clusters. The observed vibrational shifts and rotational constants are reported. There is no obvious indication of superfluid effects for para-H2 clusters up to N=13. Sharp transitions due to even larger clusters are observed, but no definite assignments are possible. Mixed (para-H2)N–(ortho-H2)M–N2O cluster line positions can be well predicted by linear interpolation between the corresponding transitions of the pure clusters.</p>
en-copyright=
kn-copyright=

en-aut-name=TangJian
en-aut-sei=Tang
en-aut-mei=Jian
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=McKellarRobert A
en-aut-sei=McKellar
en-aut-mei=Robert A
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Steacie Institute for Molecular Science
en-keyword=vibrational
kn-keyword=vibrational
en-keyword=rotational
kn-keyword=rotational
en-keyword=infrared spectroscopy
kn-keyword=infrared spectroscopy
en-keyword=gas phase
kn-keyword=gas phase
en-keyword=superfluidity
kn-keyword=superfluidity
END

start-ver=1.4
cd-journal=joma
no-vol=401
cd-vols=
no-issue=
article-no=
start-page=218
end-page=221
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080629
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Formation of low-resistivity region in p-Si substrate of SiGe/Si episystem by remote-hydrogen plasma treatment
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We have studied effects of hydrogen treatment on the resistivity profile of the SiGe/Si episystem by spreading resistance (SR) method. In this paper, we present experimental findings that hydrogen treatment reduces the resistivity at a specific part in the Si substrate region. This position was confirmed to be under the interface between SiGe and Si that emerged on the bevel surface during hydrogen treatment. We investigated the depth of resistivity-reduced regions which was formed by various hydrogenating conditions and found that the region was extended to the same depth as the penetration depth of hydrogen. We concluded that the low-resistivity region was formed under the influence of hydrogen introduced from bevel surface. We attributed this resistivity reduction to formation of some defects which originally existed at the interface and diffused into Si substrate with hydrogen.</p>
en-copyright=
kn-copyright=

en-aut-name=YamashitaYoshifumi
en-aut-sei=Yamashita
en-aut-mei=Yoshifumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SakamotoYoshifumi
en-aut-sei=Sakamoto
en-aut-mei=Yoshifumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KamiuraYoichi
en-aut-sei=Kamiura
en-aut-mei=Yoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IshiyamaTakeshi
en-aut-sei=Ishiyama
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=4
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University
en-keyword=SiGe/Si
kn-keyword=SiGe/Si
en-keyword=Hydroge. Resistivity reduction
kn-keyword=Hydroge. Resistivity reduction
en-keyword=Interface
kn-keyword=Interface
END

start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=6
article-no=
start-page=923
end-page=938
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2006
dt-pub=20066
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A glycosylation island is a genetic region required for glycosylation. The glycosylation island of flagellin in Pseudomonas syringae pv. tabaci 6605 consists of three orfs: orf1, orf2 and orf3. Orf1 and orf2 encode putative glycosyltransferases, and their deletion mutants, Delta orf1 and Delta orf2, exhibit deficient flagellin glycosylation or produce partially glycosylated flagellin respectively. Digestion of glycosylated flagellin from wild-type bacteria and non-glycosylated flagellin from Delta orf1 mutant using aspartic N-peptidase and subsequent HPLC analysis revealed candidate glycosylated amino acids. By generation of site-directed Ser/Ala-substituted mutants, all glycosylated amino acid residues were identified at positions 143, 164, 176, 183, 193 and 201. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) analysis revealed that each glycan was about 540 Da. While all glycosylation-defective mutants retained swimming ability, swarming ability was reduced in the Delta orf1, Delta orf2 and Ser/Ala-substituted mutants. All glycosylation mutants were also found to be impaired in the ability to adhere to a polystyrene surface and in the ability to cause disease in tobacco. Based on the predicted tertiary structure of flagellin, S176 and S183 are expected to be located on most external surface of the flagellum. Thus the effect of Ala-substitution of these serines is stronger than that of other serines. These results suggest that glycosylation of flagellin in P. syringae pv. tabaci 6605 is required for bacterial virulence. It is also possible that glycosylation of flagellin may mask elicitor function of flagellin molecule.
en-copyright=
kn-copyright=

en-aut-name=TaguchiFumiko
en-aut-sei=Taguchi
en-aut-mei=Fumiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakeuchiKasumi
en-aut-sei=Takeuchi
en-aut-mei=Kasumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatohEtsuko
en-aut-sei=Katoh
en-aut-mei=Etsuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MurataKatsuyoshi
en-aut-sei=Murata
en-aut-mei=Katsuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SuzukiTomoko
en-aut-sei=Suzuki
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MarutaniMizuri
en-aut-sei=Marutani
en-aut-mei=Mizuri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KawasakiTakayuki
en-aut-sei=Kawasaki
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=EguchiMinako
en-aut-sei=Eguchi
en-aut-mei=Minako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KatohShizue
en-aut-sei=Katoh
en-aut-mei=Shizue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=kakuHanae
en-aut-sei=kaku
en-aut-mei=Hanae
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=YasudaChihiro
en-aut-sei=Yasuda
en-aut-mei=Chihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=InagakiYoshishige
en-aut-sei=Inagaki
en-aut-mei=Yoshishige
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=ShiraishiTomonori
en-aut-sei=Shiraishi
en-aut-mei=Tomonori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=IchinoseYuki
en-aut-sei=Ichinose
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

affil-num=1
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=National Institute of Agrobiological Sciences

affil-num=4
en-affil=
kn-affil=National Institute of Agrobiological Sciences

affil-num=5
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=6
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=7
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=8
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=9
en-affil=
kn-affil=National Institute of Agrobiological Sciences

affil-num=10
en-affil=
kn-affil=National Institute of Agrobiological Sciences

affil-num=11
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=12
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=13
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=14
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University

affil-num=15
en-affil=
kn-affil=The Graduate School of Natural Science and Technology, Okayama University
en-keyword=Gram-Negative bacteria
kn-keyword=Gram-Negative bacteria
en-keyword=Posttranslational modification
kn-keyword=Posttranslational modification
en-keyword=Protein Glycosylation
kn-keyword=Protein Glycosylation
en-keyword=Perception
kn-keyword=Perception
en-keyword=Aeruginosa
kn-keyword=Aeruginosa
en-keyword=Cells
kn-keyword=Cells
en-keyword=Specificity
kn-keyword=Specificity
en-keyword=Expression
kn-keyword=Expression
en-keyword=Plasmids
kn-keyword=Plasmids
en-keyword=Pathways
kn-keyword=Pathways
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=3
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20059
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Structure of spherical Yukawa clusters: A model for dust particles in dusty plasmas in an isotropic environment
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The structure of spherical clusters composed of Yukawa particles is analyzed by molecular dynamics simulations and theoretical approaches as a model for dust particles in dusty plasmas in the isotropic environment. The latter condition is expected to be realized under microgravity or by active cancellation of the effect of gravity on the ground. It is found that, at low temperatures, Yukawa particles form spherical shells and, when scaled by the mean distance, the structure is almost independent of the strength of screening including the case of the Coulomb interaction. The positions and populations of shells and the conditions for the change of the number of shells are expressed by simple interpolation formulas. Shells have an approximately equal spacing close to that of triangular lattice planes in the bulk close-packed structures. It is shown that, when the cohesive energy in each shell is properly taken into account, the shell model reproduces the structure of spherical Yukawa clusters to a good accuracy.</p>
en-copyright=
kn-copyright=

en-aut-name=TotsujiHiroo
en-aut-sei=Totsuji
en-aut-mei=Hiroo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OgawaTakafumi
en-aut-sei=Ogawa
en-aut-mei=Takafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TotsujiChieko
en-aut-sei=Totsuji
en-aut-mei=Chieko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TsurutaKenji
en-aut-sei=Tsuruta
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University
en-keyword=coulomb crystals
kn-keyword=coulomb crystals
en-keyword=simulation
kn-keyword=simulation
en-keyword=energy
kn-keyword=energy
en-keyword=charge
kn-keyword=charge
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=5
article-no=
start-page=1725
end-page=1736
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19580531
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the Active Estrogen in the Urine of Uterine Cancer Patient
kn-title=子宮癌患者の尿中活性Estrogenに就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=For a quite long time a considerable attention has been focused on the relationship between the etiology of cancer and estrogen, and experimental and clinical studies have been conducted from various angles. However, studies on this problem by a direct quantitative analysis of estrogen are still meager. The methods of quantitative analysis of estrogen, on the other hand, namely, both the biological and the chemical determinations, have recently been so improved that it is now possible to conduct microdetection of estrogen. Moreover, by chemical determination estrogne can now be estimated so that the actual estrogen metabolism in vivo is being clarified. Therefore, an attempt has been made to explore the relationship between cancer and estrogen. First women over three years past menopause were selected so as to avoid the effect of variations in menstrual period as well as the effect of fluctuations in estrogen at menopause. Active estrogen in urine was then determined in order to estimate indirectly the active estrogen in vivo. These procedures were undertaken for both the non-cancer and the cancer cases for the comparison: and at the same time the active estrogen in cases with cervical cancer were studied in detail by dividing the duration of cancer progress into various stages. The purpose of this paper is to present the findings obtained from the foregoing study. 1) As for the sensitivity of the biological microdetection method devised by us and applied in experimental animals that proved to be over 60 per cent positive, it has been possible to detect 0.00075 γ estradiol. Moreover, in the application of this method for comparative study of estrogen excreted into urine, it has been found that the use of urine during 12 hours at night is simpler and more adequate. 2) As for the average estrogen quantity, cases with uterine cancer tend to show an increase as compared with that in non-cancer cases; in comparing solely the cases with cervical cancer the quantity likewise tends to show an increase in contrast with non-cancer cases; and in the cases with cervical cancer at various progressive stages it has been observed that the further advanced the cancer the greater tends to be the estrogen excreted in urine. 3) Among the patients with cervical cancer, only 17.6 per cent showed a marked abnormality in estrogen as compared with non-cancer cases; and the majority of them showed estrogen distribution within the range of that in non-cancer cases. 4) Excepting these abnormally increased cases, and designating those that clearly surpassed the average of estrogen content over the control (non-cancer group) as a relatively increased group, the rate of incidence of this relatively-increased group is higher in the cases with cervical cancer than that in the control; and the rate of such incidence is higher in a more advanced case. 5) However, even in the cases of cervical cancers in stage Ⅱ and stage Ⅲ, there were some cases showing about the same proportion of relatively low quantity of estrogen as in the control; and these results rather coincided accidentally with those of disturbances observable in the liver function in the cervical cancer as already reported in the literature. In addition, in a single case in which the quantity of estrogen has been possible to compare with histological findings of ovarian lesions, as no appreciable decrease in estrogen quantity can be observed in comparison with the findings of ovarian lesions, it is assumed that the causative factor for estrogen increment exists outside the ovarian lesions. As for the causative factor the functions of the liver and of the adrenal cortex seem to play important role. However, as can be assumed from the results of experiments so far described, the disturbances in the liver functions appear to play a principal role in elevating the quantity of active estrogen in urine of cervical cancer patient. 6) As regards the relationship between the pattern of cancer growth and the size of visible part of carcinogenic focus on one hand and the quantity of estrogen on the other, there is nothing especially noteworthy to mention.
en-copyright=
kn-copyright=

en-aut-name=MoriKumehiko
en-aut-sei=Mori
en-aut-mei=Kumehiko
kn-aut-name=森久米彦
kn-aut-sei=森
kn-aut-mei=久米彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部産婦人科教室
END

start-ver=1.4
cd-journal=joma
no-vol=86
cd-vols=
no-issue=9-10
article-no=
start-page=415
end-page=444
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1974
dt-pub=19741030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinical studies on application of the speech aid to speech therapy of cleft palate
kn-title=口蓋裂患者の言語治療における発音補助装置（Speech aid）の応用に関する臨床的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=I conducted clinical studies on application of the speech aid for the purpose of improving nasopharyngeal function in those patients who still had speech disturbance due to nasopharyngeal insufficiency even after palatal repair. For the treatment of speech disturbance accompanying cleft palate, it is necessary to perform palatal repair to restore nasopharyngeal function to its normal level as early as possible and also to give systematic speech therapy. However, we encounter not infrequently cases with residual speech difficulty due to nasopharyngeal insufficiency even after such palatal repair. For this reason, I carried out clinical studies on 64 cases by applying the speech aid in order to improve their nasopharyngeal function and giving speech therapy. As a result I have drawn the conclusions as follows. 1. For the improvement of nasopharyngeal function and normalization of speech, the speech aid seemed to be indicated for those those patients with expiration loss rate of over 2.1 at the time of blowing with Taguchi's manometer. 2. When the speech aid applied was classified into four types according to the morphology and function of its palatomaxillar section, there were 36 cases (56.2% ) for whom the palatomaxillar section was used for the sole purpose of supporting the pharyngeal section, but in the cases of bilateral and unilateral cleft lip and palate patients there were 63.4% of them for whom speech aid was used for restore the tooth defect and hard palate perforation. 3. Among 29 cases whose cephalometric x-ray films were taken at the time of pronouncing the vowel "a", the cases showing Passavant's bar amounted to 66.5% , where the upper margin of Passavant's bar was in contact with the posterior inferior edge of the pharyngeal section, which coincided with the produced under the direct view of mirror, but in those cases not revealing Passavant's bar there could be observed no definite position-relationship. 4. With growth of the jaw the speech aid applied in 3-5 year old children had to be reconstructed in the majority of cases, and the duration of time from the first speech aid insertion to the first reconstruction set ranged 19 months to 23 months. 5. The speech aid proved to bo effective on nasopharyngeal function in 85.9% of the cases applied, bringing up the respiration loss to O point at the blowing time, which in the majority of them had been attained within 4 months after the application of speech aid. Especially marked was such an effect in those cases whose palato-pharyngeal sphincter function before the application was good, some even showing an immediate effect. 6. As to the speech improvement after the application of speech aid, there were 41.7% , of the cases recovering to normal speech level (degree one), and 22.9% of them who could carry on normal speech (degree two), revealing the application to be most effective in the age range of 3 to 6 years old. 7. As for the relation of speech improvement to repiration loss rate, in the cases whose respiration loss rate recovered to O after the application of speech aid there were 48.8% whose speech improvement reached degree one and 26.8% of them degree two, but in all those whose respiration loss rate did not recover to O, there was recognized a residual cleft palate speech (degree 3) that existed prior to the application of speech aid. 8. After the application of speech aid it required a speech therapy suitable to each individual in a narrow sence for a certain length of time, and most of the patients over one year old but under two years had to be given speech therapy. 9. There were some cases of auditory disturbance with slight hearing difficulty that seemed to affect the speech recovery, hence cases with auditory disturbance require oto-rhinologic therapy. 10. It had been shown that the application of speech aid inhibits speech recovery effect in the cases of markedly low intelligence so that the speech aid was preferably indicated for those with intelligence of over (IQ=80). 11. Factors contributing to the interference of speech recovery were mental disturbance, auditory disturbance and poor family environments, and it is essential to discover the most suitable methods to eradicate these factors or to develop speech therapy most suitable for individual cases. 12. In the cases of unoperated submucous cleft palate, speech aid proved to be effective, for those cases of relatively young age with a good palato-pharyngeal sphineter function. 13. We tried adjustments of speech such as reduction of pharyngeal section by scraping on 20 cases whose speech had recovered to normal, and we found 5 cases whose speech aid could be removed, 8 cases whose pharyngeal section had shrunken, and 7 cases whose one could not be reduced in size. 14. The cases removed of speech aid were lower aged-children and had the good function of the palato-pharyngeal sphincter and the sufficient length of soft palate, and by the age of 7 years both the anterior to posterior diameter and the left-to-right diameter of the pharyngeal section had shrunken to 5 or 6mm and they showed the shrinkage rate of less than 1/2 in comparing that before and after scraping. Macroscopic examinations of these cases revealed the enhancement of the palato-pharyngeal sphincter function, especially marked was the functional improvement of the pharyngeal lateral wall in the majority of cases. In addition, the cephalometric x-ray films showed an improved movement of soft palate, a marked Passavant's bar, and the shortening of the minimum distance between the soft palate and Passavant's bar. 15. From these findings it seemed that in order to achieve the most ideal result with speech aid we should plant to restore speech level to its normal at first with speech aid, then to scrape the pharyngeal section gradually while observing patient's speech manner, and finally to try to remove the speech aid as soon as feasible.
en-copyright=
kn-copyright=

en-aut-name=FujiwaraHiroaki
en-aut-sei=Fujiwara
en-aut-mei=Hiroaki
kn-aut-name=藤原弘旦
kn-aut-sei=藤原
kn-aut-mei=弘旦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部口腔外科学教室
END

start-ver=1.4
cd-journal=joma
no-vol=121
cd-vols=
no-issue=3
article-no=
start-page=173
end-page=175
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=20091201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Relief of cancer pain in a non small cell lung cancer patient by a polyhedral approach
kn-title=多角的アプローチにより癌性疼痛のコントロールを得ることができた非小細胞肺癌の一例
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We report a case of cancer-related pain relieved by a polyhedral approach. A woman in her late 30s with advanced non small cell lung cancer suffered from back pain caused by the cancer invasion to a thoracic vertebra. She could not take a sufficient dose of opioid due to its adverse effects. A supplementary analgesic was not found to be effective. Palliative radiation was considered desirable, but she could not maintain a dorsal position for irradiation due to back pain. Continuous epidural anesthesia was then introduced. Epidural anesthesia allowed her to lie in a spine position for radiation therapy. After completion of radiation therapy, her back pain was relieved with a low dose of transdermal fentanyl without epidural anesthesia.
en-copyright=
kn-copyright=

en-aut-name=IchiharaEiki
en-aut-sei=Ichihara
en-aut-mei=Eiki
kn-aut-name=市原英基
kn-aut-sei=市原
kn-aut-mei=英基
aut-affil-num=1
ORCID=

en-aut-name=MatsuokaJunji
en-aut-sei=Matsuoka
en-aut-mei=Junji
kn-aut-name=松岡順治
kn-aut-sei=松岡
kn-aut-mei=順治
aut-affil-num=2
ORCID=

en-aut-name=TakigawaNagio
en-aut-sei=Takigawa
en-aut-mei=Nagio
kn-aut-name=瀧川奈義夫
kn-aut-sei=瀧川
kn-aut-mei=奈義夫
aut-affil-num=3
ORCID=

en-aut-name=MatsuzakiTakashi
en-aut-sei=Matsuzaki
en-aut-mei=Takashi
kn-aut-name=松崎孝
kn-aut-sei=松崎
kn-aut-mei=孝
aut-affil-num=4
ORCID=

en-aut-name=KatsuiKuniaki
en-aut-sei=Katsui
en-aut-mei=Kuniaki
kn-aut-name=勝井邦彰
kn-aut-sei=勝井
kn-aut-mei=邦彰
aut-affil-num=5
ORCID=

en-aut-name=KiuraKatsuyuki
en-aut-sei=Kiura
en-aut-mei=Katsuyuki
kn-aut-name=木浦勝行
kn-aut-sei=木浦
kn-aut-mei=勝行
aut-affil-num=6
ORCID=

en-aut-name=TanimotoMitsune
en-aut-sei=Tanimoto
en-aut-mei=Mitsune
kn-aut-name=谷本光音
kn-aut-sei=谷本
kn-aut-mei=光音
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　緩和医療学講座

affil-num=2
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　緩和医療学講座

affil-num=3
en-affil=
kn-affil=岡山大学病院　血液・腫瘍・呼吸器・アレルギー内科

affil-num=4
en-affil=
kn-affil=岡山大学病院　麻酔科蘇生科

affil-num=5
en-affil=
kn-affil=岡山大学病院　放射線科

affil-num=6
en-affil=
kn-affil=岡山大学病院　血液・腫瘍・呼吸器・アレルギー内科

affil-num=7
en-affil=
kn-affil=岡山大学病院　血液・腫瘍・呼吸器・アレルギー内科
en-keyword=癌性疼痛 (cancer-related pain)
kn-keyword=癌性疼痛 (cancer-related pain)
en-keyword=硬膜外ブロック　(epidural anesthesia)
kn-keyword=硬膜外ブロック　(epidural anesthesia)
en-keyword=放射線療法 (radiation therapy)
kn-keyword=放射線療法 (radiation therapy)
END

start-ver=1.4
cd-journal=joma
no-vol=93
cd-vols=
no-issue=3-4
article-no=
start-page=327
end-page=341
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1981
dt-pub=19810430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A study on the position of chest electrodes for the Frank lead system Part II. A clinical study
kn-title=Frank誘導法の胸部電極の位置に関する研究 第2編 臨床的検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vectorcardiograms with the Frank lead system were recorded in 122 healthy subjects (65 men, 57 women) and the effect of horizontal displacement of chest electrodes C, A and I on the vectorcardiogram was studied. The results obtained were as follows: 1) With electrode C displaced 2 cm to the right of the original position, Rz, Sz, and Tz increased, the azimuthal angle of QRSmax deviated backward and the QRS-T angle increased. Almost contrary changes were observed with displacement 2 cm to the left. 2) Rx, Tx, QRSmax and Tmax increased with simultaneous forward dislocation of electrodes A and I, but decreased with backward displacement of the electrodes. A greater influence on the direction of vectors was caused by shifting the electrodes backward. However, the elevation of QRSmax, the azimuthal angle of Tmax and spatial QRS-T angle changed slightly. 3) Changes greater than 1.0 mV and 10 % in one of the parameters (Rx, Rz, Sx, QRSmax, Tx, Tz, Tmax) were observed in 35-45 % of the subjects with a 2 cm horizontal displacement of electrode C, while an identical displacement of electrodes A and/or I caused the changes in more than 80 % of the subjects. 4) Therefore, in clinical use, it is acceptable to vary the position of electrode C horizontally within 1 cm from the original position in man and within 2 cm in women.
en-copyright=
kn-copyright=

en-aut-name=MiyasakaMinoru
en-aut-sei=Miyasaka
en-aut-mei=Minoru
kn-aut-name=宮阪実
kn-aut-sei=宮阪
kn-aut-mei=実
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
en-keyword=Frank誘導法
kn-keyword=Frank誘導法
en-keyword=胸部電極
kn-keyword=胸部電極
en-keyword=ベクトル心電図
kn-keyword=ベクトル心電図
en-keyword=QRS環
kn-keyword=QRS環
en-keyword=T環
kn-keyword=T環
END

start-ver=1.4
cd-journal=joma
no-vol=1
cd-vols=
no-issue=1
article-no=
start-page=13
end-page=22
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1966
dt-pub=19660331
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=State of Efflux of Scavenging Air through the Scavenging Ports
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to make clear how the efflux angle changes in a small crankcase scavenged engine cylinder and in a large loop scavenged engine cylinder, the inlet flow pattern in the single cycle model cylinder has been observed by a high speed motion camera. In general, the scavenging air stream is not effluent in the designed direction of the scavenging port at comparatively slight opening, and the efflux angle changes in proportion to port opening advance. In a small crankcase scavenged engine cylinder, to keep the scavenging air stream in the direction of the scavenging port it is effective to incline the scavenging air passage between the crankcase and the cylinder, and to make thicker the cylinder wall where located scavenging port. In a large loop scavenged engine cylinder to coincide the scavenging air stream with the direction of the scavenging port in the early stage of the scavenging, it has an effect to set the guide plate the position of 1/2 at port height.
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=KashiwadaYukio
kn-aut-sei=Kashiwada
kn-aut-mei=Yukio
aut-affil-num=1
ORCID=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=AkiyamaKoichi
kn-aut-sei=Akiyama
kn-aut-mei=Koichi
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Mechanical Engineering School of Engineering, Okayama University

affil-num=2
en-affil=
kn-affil=Department of Mechanical Engineering School of Engineering, Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=26
cd-vols=
no-issue=2
article-no=
start-page=77
end-page=92
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=19920328
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Beam Focusing Antenna for the TE(0)n Mode High-Power Millimeter Wave
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This paper describes a method to design an antenna to focus millimeter-wave beam generated by a gyrotron. The antenna, which has been proposed by the authors, consists of a stair-cut circular waveguide and two cylindrical reflectors; one is elliptic and the other is parabolic. Its principle is based on the geometrical optics though slightly modified to consider the diffraction effect. Results of low-power experiments agree well with the design on beam direction, beam width and the position of the focal point. At 35.5 GHz using TE(01) mode, a focused beam with half-power thickness of 13 mm x 10 mm was obtained. This type of antennas find applications to millimeter-wave scattering measurement in fusion plasma research and high- energy-density source for material heating.
en-copyright=
kn-copyright=

en-aut-name=WadaOsami
en-aut-sei=Wada
en-aut-mei=Osami
kn-aut-name=和田修己
kn-aut-sei=和田
kn-aut-mei=修己
aut-affil-num=1
ORCID=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=NakajimaMasamitsu
kn-aut-sei=Nakajima
kn-aut-mei=Masamitsu
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Electrical and Electronic Engineering

affil-num=2
en-affil=
kn-affil=Department of Electronics, Kyoto University
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=5-6
article-no=
start-page=601
end-page=608
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=1992
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Renal hemodynamics in improvement of diabetic nephropathy in stage Ⅲ and Ⅳ
kn-title=Ⅲ, Ⅳ期糖尿病性腎症の改善における腎血行動態に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Diabetic nephropathy is characterized by specific morphological changes and impairment of renal hemodynamics. To determine whether diabetic nephripathy could be improved by improvement of renal hemodynamics, 80μg/day of prostaglandin E(1) (PGE(1)) was administered daily for 4 weeks to 19 non-insulin-dependent diabetic inpatients with overt proteinuria. In 6 patients who initialy had daily urinary protein (UP) values above 3.5g/day, the UP value was sinificantly reduced by PGE(1) administration (p<0.05). However, in 6 patients who initially had UP values above 200mg/day and below 3.5g/day, the UP value was unchanged. In the former group, creatinine clearance was significantly reduced during PGE(1) administration (p<0.05), but was restored after termination of PGE1 adminstration (ns). Serum creatinine, serum total protein and serum albumin values were unchanged by PGE(1) administration in both groups. One petient who had a UP level above 200mg/day showed marked improvement on his renogram during PGE(1) administration and in 7 patiests who had UP values below 200mg/day, the ratio of plasma renin activity 120 minutes after furosemide injection in an upright posture to that of basal condition (PRA120/0) was significantly reduced. These findings inducate that PGE(1) increased renal blood flow, and suggest that improvement of renal hemodynamics is an important method of therapy for diabetic nephropathy with overt proteinuria.
en-copyright=
kn-copyright=

en-aut-name=IchikiKen
en-aut-sei=Ichiki
en-aut-mei=Ken
kn-aut-name=市木研
kn-aut-sei=市木
kn-aut-mei=研
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科学教室
en-keyword=糖尿病性腎症
kn-keyword=糖尿病性腎症
en-keyword=蛋白尿
kn-keyword=蛋白尿
en-keyword=腎血行動態
kn-keyword=腎血行動態
en-keyword=プロスタグランディンE(1)
kn-keyword=プロスタグランディンE(1)
en-keyword=糸球体過剰濾過
kn-keyword=糸球体過剰濾過
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=1-2
article-no=
start-page=147
end-page=159
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1991
dt-pub=1991
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Neurophysiological study of deep brain stimulation for the treatment of deafferentation pain Part 2. Usefulness of deep cerebral sensory evoked potentials (SEP) for assessment of traget points in the lemniscal system
kn-title=Deafferentation pain に対する脳深部刺激療法の研究 第2編― 内側毛帯系刺激における除痛点の脳深部知覚誘発反応（SEP）による評価について―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=To relieve deafferentation pain, electrophysiological assessment of target points was performed using deep cerebral SEPs. As a preliminary experiment, the relationship between the waveform of the SEP and the anatomical structure of the brain was examined in cats. The infraorbital nerve was stimulated and the SEP was recorede around the VPM. From the rostral edge of the medial leminiscus (ML), a high voltage positive wave was recorded, and from the ventral side of the VPM a wide negative wave with positive wavelets was recorded. For the treatment of three cases of thalamic pain, the deep cerebral SEP was recorded during the insertion of a chronic electrode for deep brain stimulation. By contralateral median nerve stimulation, the high voltage posotive waves (peak latency ; 13-15msec) from the ML and the subsequent unerven waves from the VPL were recorded along the trajectory. The later uneven waves were composed of wide negative waves and positive wavelets, which were thought to be derived from the synaptic activity of the VPL and thalamocortical rediation, respectively. Effective pain  relief was obtained at the border zone of the ventral VPL and the ML, where the characteristic waves of the VPL changed to those of the ML. The deep cerebral SEP can be used for the identification of target points electrophysiologically.
en-copyright=
kn-copyright=

en-aut-name=TomitaSusumu
en-aut-sei=Tomita
en-aut-mei=Susumu
kn-aut-name=富田享
kn-aut-sei=富田
kn-aut-mei=享
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部脳神経外科学教室
en-keyword=deafferentation pain
kn-keyword=deafferentation pain
en-keyword=deep brain stimulation
kn-keyword=deep brain stimulation
en-keyword=sensory evoked potential
kn-keyword=sensory evoked potential
en-keyword=leminiscus medialis
kn-keyword=leminiscus medialis
en-keyword=nucleus ventrocaudalis
kn-keyword=nucleus ventrocaudalis
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=9-10
article-no=
start-page=897
end-page=904
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=199210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on cell biology and chemotherapy of lung cancer using tissue culture techniques Part 1. Drug sensitivity test in lung cancer using human tumor clonogenic assay
kn-title=組織培養法を用いた肺癌の細胞生物学並びに治療に関する研究 第1編 Human Tumor Clonogenic Assay を用いた制癌剤感受性試験の検討―肺癌を中心として―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The selection of a series of effective drugs for individual patients in advance of drug therapy should increase the success of cancer chemotherapy. The human tumor clonogenic assay was evaluated as a drug sensitivity test mainly in patients with lung cancer. Tumor cells from malignant pleural effusion, tumor-positive bone marrow aspirates, and tumor tissues from the primary or metastases were used as sepcimens. Prior to plating, tumor cells were exposed to 4-hydroperoxy ifosfamide, adriamycin, mitomycin C, methotrerxate, and cisplatin for one hour at graded concentrations which were achievable in man. Of 151 specimens tested, 93(62%) yielded at least 5 colonies in the control plates containing no durgs. Colony growth (≧5/plate) was seen in 80% of squamous cell carcinoma, in 73% of small cell carcinoma, in 62% of adenocarcinoma, and in 40% of large cell carcinoma. Among the 93 specimens with colony growth, 62 yielded more than 30 colonies in the control plates and were put in force for drug sensitivity testing. Of 37 instances in which the clinical response to a certain drug was examined, 34(92%) showed an in vitro-in vivo correlation, showing a true positive rate of 57% and a true negative rate of 100%. In summary, the human tumor clonogenic assay would be an excellent technique for testing the drug sensitivity of the tumor in individual patients tumor.
en-copyright=
kn-copyright=

en-aut-name=KishimotoNobuyasu
en-aut-sei=Kishimoto
en-aut-mei=Nobuyasu
kn-aut-name=岸本信康
kn-aut-sei=岸本
kn-aut-mei=信康
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=human tumor clonogenic assay
kn-keyword=human tumor clonogenic assay
en-keyword=drug sensitivity
kn-keyword=drug sensitivity
en-keyword=lung cancer
kn-keyword=lung cancer
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=1-2
article-no=
start-page=51
end-page=56
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=1992
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinicopathological studies in patients with colorectal cancers without lymphnode metastasis
kn-title=リンパ節転移を認めない大腸癌の臨床病理学的検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The clinicopathologic findines and the prognosis of 154 patients without regional lymph nodes metastasis or distant metastasis (n(-)group) were compared with those in 134 patients with regional lymph nodes metastasis and without distant netastasis (n(+)group). The mean age of the n(-) group was significantly younger than that of the n(+) group (p<0.05) and there was no significant sexual preponderance. The proportion of the cases without lymphatic vessel invasion in the n(-) group was significantly higher than in the n(+) group (p<0.01). The proportion of the cases without venous invasion was significantly higher in the n(-) group than in the n(-) group (p<0.05). There was no difference in cumulative surval rate of patients in then(-) group than in the n(+) group (p<0.05). There was no difference in cumulative survial rate of patients in the n(-) group in respect to the background factors such as sex, tumor site, and venous invasion. However, the survial rate of patients whose cancer invasion was limited within the mucosa (m), submucosa (sm) and musclar propria (pm) was significantly better than that of patients who had subserosal or subadventitial penetration (ss(al)), serosal or adeventitial penetration (s(a2)) and cancer invasion adjacent organs (si(ai))(p<0.05). The survial rate of patients with lymphatic vessel invasion was significantly higher than that of patients without lymphatic vessel invasion (p<0.05). That the poor prognostic factors in n(-) colorectal cancer patients are cancer penetration to/beyond ss(al), or positive lymphatic vessal invasion.
en-copyright=
kn-copyright=

en-aut-name=ShiikiShigeo
en-aut-sei=Shiiki
en-aut-mei=Shigeo
kn-aut-name=椎木滋雄
kn-aut-sei=椎木
kn-aut-mei=滋雄
aut-affil-num=1
ORCID=

en-aut-name=FuchimotoSadanori
en-aut-sei=Fuchimoto
en-aut-mei=Sadanori
kn-aut-name=淵本定儀
kn-aut-sei=淵本
kn-aut-mei=定儀
aut-affil-num=2
ORCID=

en-aut-name=IwagakiHiromi
en-aut-sei=Iwagaki
en-aut-mei=Hiromi
kn-aut-name=岩垣博巳
kn-aut-sei=岩垣
kn-aut-mei=博巳
aut-affil-num=3
ORCID=

en-aut-name=HamadaHiroshi
en-aut-sei=Hamada
en-aut-mei=Hiroshi
kn-aut-name=浜田史洋
kn-aut-sei=浜田
kn-aut-mei=史洋
aut-affil-num=4
ORCID=

en-aut-name=HizutaAkio
en-aut-sei=Hizuta
en-aut-mei=Akio
kn-aut-name=日伝晶夫
kn-aut-sei=日伝
kn-aut-mei=晶夫
aut-affil-num=5
ORCID=

en-aut-name=OiritaKunzo
en-aut-sei=Oirita
en-aut-mei=Kunzo
kn-aut-name=折田薫三
kn-aut-sei=折田
kn-aut-mei=薫三
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部第一外科学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部第一外科学教室

affil-num=4
en-affil=
kn-affil=岡山大学医学部第一外科学教室

affil-num=5
en-affil=
kn-affil=岡山大学医学部第一外科学教室

affil-num=6
en-affil=
kn-affil=岡山大学医学部第一外科学教室
en-keyword=大腸癌
kn-keyword=大腸癌
en-keyword=リンパ節転移
kn-keyword=リンパ節転移
en-keyword=n(0)
kn-keyword=n(0)
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=3-4
article-no=
start-page=275
end-page=285
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=1994
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on human bone marrow stromal cells Part 2. Growth and composition of human bone marrow stromal cells in chronic myelogenous leukemia (CML), myelodysplastic syndrome (MDS) and aplastic anemia (AA)
kn-title=ヒト骨髄間質細胞に関する研究　第2編 慢性骨髄性白血病, 骨髄異形成症候群, 再生不良性貧血におけるヒト骨髄間質細胞の増殖動態並びに細胞構成の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Growth and composition of human bone marrow stromal cells (HBMSC) in CML, MDS and AA were studied by using modified Dexter's culture system. Growth was experssed by the percent of HBMSC at the bottom of the culture dish(% confluence) at the 1st, 2nd, 3rd and 4th week. Cells harvested at the 4th week, were identified by monoclonal antibodies (anti-fibronectin, anti-vWF, anti-CD14, anti-CD7, anti=CD4 and anti-CD8 antibodies) and oil red 0 staininig method.
 In normal healty individuals (NC), % confluence at the 1st, 2nd, 3rd and 4th week were 74±4%, 82±13%, 100% and 100% respectively (n=10). HBMSC were composed of 64±4% fibro-blastoid cells, 15±3% endothelial cells, 9±4% monocyte/macrophage, 3±2% fat cells, 7±3% T lymphocyte, 4±1% helper/inducer T cells and 4±1% suppressor/cytotoxic T cells, respec-tively (n=8). In CML (n=6), % confluence at each week were almost on the decrease in comparison with those in NC and the cells tended to be detached from the bottom at the 3rd and 4th weeks. The composition of the HBMSC were not different in comparison with those in NC. In MDS (n=4), % confluence at each week and composition of HBMSC were not different in comparison with those in NC. In 3 of the 4 AA, % confluence at each week were not different in comparison with those in NC. Fat cells were markedly increased in one of 4 cases, which clinically belonged to the severe type of AA. In the other 3 AA, the percentage of endothelial cells was increased up to 35%, 32% and 21%, respectively. The CD4/CD8 rations were not different from those in NC.
 These finding indicate that the pathophysiological analysis of hematological disorders should be done not only on hematopoietic stem cells but also on the hematopoietic microenvi-ronment including bone marrow stromal cells.
en-copyright=
kn-copyright=

en-aut-name=MatsuzakiToshiaki
en-aut-sei=Matsuzaki
en-aut-mei=Toshiaki
kn-aut-name=松﨑敏朗
kn-aut-sei=松﨑
kn-aut-mei=敏朗
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=Human bone marrow stromal cells
kn-keyword=Human bone marrow stromal cells
en-keyword=Long-term bone marrow culture
kn-keyword=Long-term bone marrow culture
en-keyword=Chronic myelogenous leukemia
kn-keyword=Chronic myelogenous leukemia
en-keyword=Myelodysplastic syndrome
kn-keyword=Myelodysplastic syndrome
en-keyword=Aplastic anemia
kn-keyword=Aplastic anemia
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=11-12
article-no=
start-page=1105
end-page=1116
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=199412
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of fentanyl anesthesia on the canine excised cross-circulated heart preparation system
kn-title=イヌ摘出交叉灌流心臓標本実験系におけるフェンタニール麻酔の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Sodium pentobarbital has been used as a representative anesthetic in the experimental system using the canine excised cross-circulated heart preparaton. In the present study, fentanyl was established as a new anesthetic in this experimental system. The mechanoenergetic effects of fentanyl on the excised heart were investigated by using the framework of Emax (the slope of the end-systolic pressure-volume relation), PVA (Pressure-volume area) and VO2 (left ventricle oxygen consumption per beat). To determine the effects of fentanyl on the support dog, the systemic arterial blood pressure, heart rate, and plasma catecholamine concentration were measured.
In the excised heart, fentanyl did not change the slope of the PVA-VO2 relation. This indicated that the cardiac mechanoenergetics was not qualitatively changed by fentanyl. In the support dog, fentanyl decreased the heart rate, but did not change the systemic arterial blood pressure. Fentanyl anesthesia is considered to be useful as an anesthetic for the experimantal system using the canine excised cross-circulated heart preparation.
en-copyright=
kn-copyright=

en-aut-name=IshiokaKazunari
en-aut-sei=Ishioka
en-aut-mei=Kazunari
kn-aut-name=石岡一成
kn-aut-sei=石岡
kn-aut-mei=一成
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部麻酔・蘇生学教室
en-keyword=麻薬
kn-keyword=麻薬
en-keyword=フェンタニール
kn-keyword=フェンタニール
en-keyword=Emax
kn-keyword=Emax
en-keyword=PVA
kn-keyword=PVA
en-keyword=酸素消費量
kn-keyword=酸素消費量
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=7-8
article-no=
start-page=715
end-page=731
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1993
dt-pub=199308
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=HLA-DNA-DQ typing by PCR-RFLP method in renal transplantation
kn-title=PCR-RFLP 法による HLA-DNA-DQ タイピングと腎移植
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Two methods of HLA-DNA typing (PCR-RFLP method and PCR-SSO method) were performed on HLA-DQ in living related, living unrelated and cadaveric renal transplants. These two DNA typing methods allowed more accurate and more detailed typing than the conventional typing method. The effect of DNA histocompatibility of DQA 1 and DQB 1, both typed by the PCR-RFLP method, on clinical outcome and surviving graft rate of renal transplant patients was analyzed. There was no correlation found between the number of mismatches between donor and recipient of DQA 1 typing in cadaveric renal transplants, of DQB 1 typing in cadaveric, living unrelated and living related transplants and the clinical outcome nor the surviving graft rate of renal transplant patients. Both the clinical outcome and the surviving graft rate in the group in which DQ 5 mismatch between donor and recipient was positive were statistically poorer than that in the group in which DQ 5 mismatch was negative. This result suggests the existance of DQ 5 mismatch between donor and recipient greatly influences graft survival after renal transplantation.
en-copyright=
kn-copyright=

en-aut-name=HirakawaKeiichi
en-aut-sei=Hirakawa
en-aut-mei=Keiichi
kn-aut-name=平川恵一
kn-aut-sei=平川
kn-aut-mei=恵一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科学教室
en-keyword=HLA-DNA タイピング
kn-keyword=HLA-DNA タイピング
en-keyword=PCR-RFLP 法
kn-keyword=PCR-RFLP 法
en-keyword=PCR-SSO 法
kn-keyword=PCR-SSO 法
en-keyword=腎移植
kn-keyword=腎移植
en-keyword=DQ 抗原
kn-keyword=DQ 抗原
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=1-2
article-no=
start-page=71
end-page=81
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=199402
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the soluble interleukin-2 receptor in patients with bronchial asthma
kn-title=気管支喘息におけるInterleukin-2 receptorに関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cytokine-mediated interactions among blood cells, especially T-lymphoctes, may be impor-tant in the regulation of airway inflammation in asthma. To investigate whether T-lymphocytes are related to disease activity in asthma, serum soluble interleukin-2 receptor (sIL-2R) levels were measured in 77 bronchial asthmatics and 19 healthy volunteers. The serum sIL-2R level was higher in asthmatics than in healthy controls (mean±SD ；449.8±225.5 vs. 323.2±123.9 U/ml ; p<0.01). However, sIL-2R levels were similar between atopic and non-atopic asthmatics (451.9±235.9 vs. 416.7±147.2 U/ml), and were also similar between severe and mild to moderate asthmatics (437.4±209.9 vs. 469.8±276.1 U.ml). Steroid-dependent intractable asthmatics treated with prednisolone st doses of 5-10 mg/day showd higher sIL-2R levels (470.1±257.9 U/ml) than asthmatics treated with the drug at doses below 5 mg/day (357.2±102.6 U/ml) and intractable asthmatics treated with the drug at doses above 10 mg/day (282.5±194.5 U/ml). There was a strong correlation (r=0.915 ; p<0.01) between percentage at IL-2R-positive helper T cells among peripheral white blood cells and the serum concentration of sIL-2R.
 The type Ⅳ cell-mediated immune response is considered to be related to the pathogenesis of asthman. Serum sIL-2R levels are thought to reflect the extent of T-lymphocyte activation in non-malignant disease, but the level is aiso influenced by steroid therapy.
en-copyright=
kn-copyright=

en-aut-name=KozukaAkiko
en-aut-sei=Kozuka
en-aut-mei=Akiko
kn-aut-name=小塚明子
kn-aut-sei=小塚
kn-aut-mei=明子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=難治性喘息
kn-keyword=難治性喘息
en-keyword=T細胞
kn-keyword=T細胞
en-keyword=IL-2R
kn-keyword=IL-2R
en-keyword=細胞反応型アレルギー
kn-keyword=細胞反応型アレルギー
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=1-2
article-no=
start-page=13
end-page=29
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19960229
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The expansion and antitumoral activity of recombinant IL-2 activated tumor infiltrating lymphocytes from human cancers
kn-title=腫瘍内浸潤リンパ球の rIL-2 添加培養と細胞傷害活性の研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Tumor infiltrating lymohocytes (TIL) were isolated from various human cancers and cultured. The expansion, cytotoxicity, phenotype, and perforin, which is considered the cytotoxic factor of killer cells, of TIL were examinel. TIL cultured in 1000u/ml recombinant interleukin-2 (rIL-2) with 20% conditioned medium (CoM) exhibited far better expansion capability than that of TIL cultured in rIL-2 1000u/ml alone, and continued to expand up to 14 weeks. The maximal expansion index (EI) was 23912. When TIL arrested expansion during the culture, the addition of PHA restored the ability to expand. The CoM was composed of spent medium from a 3-day culture of peripheral blood mononuclear cells from cancer patients. The lymphokine-activated killer (LAK) cells induced by the culture was utilized for clinical adoptive immunotherapy in our department. Cytotoxicity against K562 and Daudi target cells was highest at 2 to 3 weeks, and disappeared between 4 and 7 weeks of culture. The phenotype of fresh TIL was mainly CD3(+) T cells, predominantly CD4(+) cells, but CD8(+) cells became predominant in long term culture. The frequency of IL-2R(+) and HLA-DR+ cells was correlated with the expansiveness of TIL. The appearance and intensity of perforin positive cells were associated with the level of cytotoxicity of cultured TIL.
en-copyright=
kn-copyright=

en-aut-name=MatsumotoMitsuaki
en-aut-sei=Matsumoto
en-aut-mei=Mitsuaki
kn-aut-name=松本三明
kn-aut-sei=松本
kn-aut-mei=三明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科学教室
en-keyword=腫瘍内浸潤リンパ球
kn-keyword=腫瘍内浸潤リンパ球
en-keyword=recombinant interleukin-2
kn-keyword=recombinant interleukin-2
en-keyword=細胞傷害活性
kn-keyword=細胞傷害活性
en-keyword=リンパ球表面抗原
kn-keyword=リンパ球表面抗原
en-keyword=パーフォリン
kn-keyword=パーフォリン
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=2
article-no=
start-page=153
end-page=158
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=20020930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Flowcytometric analysis of HBcAg-specific lymphocyte reaction in acute hepatitis B
kn-title=B型急性肝炎における HBc 抗原特異的リンパ球反応の解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Antigen specific T-cell responeses are important for the eradication of Hepatitis B virus (HBV) in acute infection. Flow cytometric analysis of intracellular cytokines is a simple method for studying the T-cell response at the single-cell level in a short period pf time. In the present syudy, we invesfisated the hepatitis B virus (HBV)-specific T-cell response using flow cytometry. Eight patients with acute hepatitis B and 2 patients with fulminant hepatitis B were studied. Heparinized peripheral blood was incubated with recombinant HB core antigen (HBcAg), and intracytoplasmic cytokines were analyzed by flow cytometry. HBcAg-specific interferon-gamma positive CD4 T-cella were positive in 4 of 10 patients while HBcAg-specific interferon-gamma positive CD8 T-cells and interleukin-4 positive T-cells were negatiive in all patients. No intracytoplasmic cytokines were demonstrated in healthy subjects or with recombinant hepatitis C virus (HCV) antigens. HBcAg-specific CD4 T-cells could be detected by a sensitive flow cytometric analysis of the peripherad blood and the cytokine profile of these cells was Th1. These results suggest that HBcAg-specific Th1-type CD4 T-cells may play an important role in controlling viral clearance during acute HBV infection.
en-copyright=
kn-copyright=

en-aut-name=SuzukiTakahiro
en-aut-sei=Suzuki
en-aut-mei=Takahiro
kn-aut-name=鈴木貴博
kn-aut-sei=鈴木
kn-aut-mei=貴博
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
en-keyword=B型急性肝炎
kn-keyword=B型急性肝炎
en-keyword=B型劇症肝炎
kn-keyword=B型劇症肝炎
en-keyword=細胞内サイトカイン
kn-keyword=細胞内サイトカイン
en-keyword=フローサイトメトリー
kn-keyword=フローサイトメトリー
en-keyword=HBcAg
kn-keyword=HBcAg
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=1
article-no=
start-page=27
end-page=37
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=20020530
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The efficacy and toxicity resulting from recombinant HSV-tk adenoviral vector under the transcriptional control of caveolin-1 promoter for prostate cancer
kn-title=Caveolin-1プロモーターを用いたHerpes Simplex Virus-thymidine kinase遺伝子発現アデノウィルスによる前立腺癌遺伝子治療における有効性と安全性の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The efficacy and toxicity of adenoviral vectors that express Herpes Simplex Virus-thymidine kinase (HSV-tk) gene under the transcriptional control of caveolin-1 promoter  (cav.-1-tk) was compared to Cytomegalovirus (CMV) promoter or Rous sarcoma virus (RSV) promoter HSV-tk for prostate cancer. The cav.-1-tk demonstrated the strong cytotoxicity in vitro for mouse human prostate cancer cell line (178BMA) and human prostate cancer cell line (PC-3), which overxpress caveolin-1 levels. On the other hand, the cytotoxicity was weak for mouse (178PA) and human (LNCaP) prostate cancer cell line, which express low caveolin-1 levels. The antitumor efficacy was observed in each vector in vivo orthotopic model using 178BMA prostate cancer cell line. The immunohistochemical analysis results indicated that not only cancer cells but also tumor vasculature were destroyed by cav.-1-tk. The serum AST levels of cav.-1-tk was minimal change following intravenous injection. Taken together, adenovirus-mediated HSV-tk in situ gene therapy under the transcriptional control of caveolin-1 promoter may be effective and safe for prostate cancer compared to CMV or RSV promoter HSV-tk adenoviral vectors.
en-copyright=
kn-copyright=

en-aut-name=EbaraShin
en-aut-sei=Ebara
en-aut-mei=Shin
kn-aut-name=江原伸
kn-aut-sei=江原
kn-aut-mei=伸
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯学総合研究科泌尿器病態学
en-keyword=caveolin-1 promoter
kn-keyword=caveolin-1 promoter
en-keyword=prostate cancer cell line
kn-keyword=prostate cancer cell line
en-keyword=orthotopic model
kn-keyword=orthotopic model
END

start-ver=1.4
cd-journal=joma
no-vol=115
cd-vols=
no-issue=2
article-no=
start-page=101
end-page=108
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20050930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The role of overhead in the treatment of developmental dislocation of the hip:The evaluation of the position of the femoral head and the limbus shape
kn-title=先天性股関節脱臼に対する overhead traction 法の効果―大腿骨頭に位置および関節唇形態の変化―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The role of overhead traction in the treatment of development al dislocation of the hip, using two-directional srthrography before and after overhead traction,in 56 hips (47 patients) was investigated. There were 5 males (6 hips) and 42 females (50 hips). Of the 56 hips, 41 were treated before walking age and 15 after walking age. The age at the time of overhead traction ranged from 5 to 22 months (average 9 months). Mau'soriginal traction method was used in 38 hips from 1974 to 1988 and Ishida's modified method in 18 hips from 1989 to 2000, but the results were poorer with the former method. Reduction of the femoral head was considered to be obtained when it moved to the entrance of the acetabulum after overhead traction (19 of 41 hips). The shapes of the anterior and posterior portions of the limbus were corrected in 8 of 52 hips, especially in the cases treated before walking age. The superior portion of the linbus was uncharnged. According to the appearance on images, modifind overhead traction appeared to be effective in achieving reduction, but could not comoletely correct the limbus shape.
en-copyright=
kn-copyright=

en-aut-name=MatsudaKazumi
en-aut-sei=Matsuda
en-aut-mei=Kazumi
kn-aut-name=松田和実
kn-aut-sei=松田
kn-aut-mei=和実
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Study of Biofunctional Recovery and Reconstruction Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine and Dentistry
en-keyword=先天性股関節脱臼
kn-keyword=先天性股関節脱臼
en-keyword=overhead牽引
kn-keyword=overhead牽引
en-keyword=関節造影
kn-keyword=関節造影
en-keyword=整復
kn-keyword=整復
END

start-ver=1.4
cd-journal=joma
no-vol=117
cd-vols=
no-issue=2
article-no=
start-page=119
end-page=125
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2005
dt-pub=20050901
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=In vivo, Real Time Visualization of the Interaction between Perialveolar Microcirculation and Individual Alveolar Respiration　by CCD Videomicroscopy in Rats
kn-title=ペンシル型CCD 生体顕微鏡システムを用いたin vivo肺微小循環と肺胞呼吸の同時可視化
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We succeeded in visualizing in vivo perialveolar microcirculation and individual alveolar respiration in rats,by our high resolution intravital charge-coupled device videomicroscopy system. To elucidate the relevance and usefullness of our methods, we investigated their behavior 1) under control conditions, 2) during increased tidal volumes (TV), 3)during positive-end expiratory pressure (PEEP) application, and 4)during exposure to hypoxia. We recognized a sheet-like flow pattern in capillaries, and observed semi-collapsed capillaries at end-inspiration while flow continued.The latter indicate existence of“vascular waterfall phenomenon”.When TV was increased from 2.5 to 5ml, the alveolar size was increased from 30±10 to 65±18μm (n＝21, p＜0.05), and the red blood cell velocities in perialveolar capillary were significantly decreased from 910±210 to 290±140μm/sec (n＝21,p＜0.05). Following PEEP application with TV of 5ml,the alveolar diameter was increased even more to 80±20μm (n＝12,p＜0.05)and the flows of microvessels stopped temporarily at end-inspiration.We also visualized that precapillary arterioles clearly constricted from 34±6 to 28±6μm in response to hypoxia (n＝9, p＜0.05). In conclusion, the intravital pencil lens-probe videomicroscopy can be a powerful tool for in vivo observation of perialveolar microcirculation and alveolar respiration under important physiological conditions such as changing TV, application of PEEP, and exposure to hypoxia.
en-copyright=
kn-copyright=

en-aut-name=MorimotoTaro
en-aut-sei=Morimoto
en-aut-mei=Taro
kn-aut-name=森本太郎
kn-aut-sei=森本
kn-aut-mei=太郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯学総合研究科システム循環生理学
en-keyword=肺微小循環 (pulmonary microcirculation)
kn-keyword=肺微小循環 (pulmonary microcirculation)
en-keyword=Waterfall 現象 (waterfall phenomenon)
kn-keyword=Waterfall 現象 (waterfall phenomenon)
en-keyword=低酸素性肺血管収縮 (pulmonary hypoxic vasoconstriction)
kn-keyword=低酸素性肺血管収縮 (pulmonary hypoxic vasoconstriction)
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=20070323
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ホルムアルデヒドガスに曝露された医学生における臨床症状と皮膚試験反応についての前向き研究
kn-title=A prospective study of clinical symptoms and skin test reactions in medical students exposed to formaldehyde gas
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Previous investigators have reported the occurrence of both allergic and non-allergic systemic complications due to exposure to formaldehyde gas. However, little is known about the pathogenic link between formaldehyde-induced clinical symptoms and patch test results, or about the long-term effects of formaldehyde exposure. In the present study, a questionnaire was administered to 143 medical students, and 60 of them were tested by patch test for formaldehyde at the beginning and end of a human anatomy laboratory course. Another group of 76 students who had finished the course 2-4 years previously were administered another questionnaire, and the patch test was carried out on 58 of them. The frequencies of skin irritation, eye soreness, lacrimation, eye fatigue, rhinorrhea, throat irritation, general fatigue and mood swings increased after repeated exposure. Two (3.3%) of 60 students became positive to 1% formaldehyde at the end of the anatomy course (one male with allergic hand dermatitis due to direct contact with formaldehyde, and one female with an atopic background with unbearable physical symptoms) while the remaining 58 showed a negative reaction throughout the study period. The vast majority of students complained of various non-allergic, physical symptoms, and recovered from such symptoms without subsequent complications. No progression to multiple chemical sensitivity was found. Students with an episode of atopic dermatitis and allergic rhinitis were susceptible to formaldehyde exposure, and developed mucocutaneous symptoms, probably due to the impaired barrier function and remodeling of the skin and mucosa.
en-copyright=
kn-copyright=

en-aut-name=TakahashiSachiko
en-aut-sei=Takahashi
en-aut-mei=Sachiko
kn-aut-name=髙橋祥子
kn-aut-sei=髙橋
kn-aut-mei=祥子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=barrier function
kn-keyword=barrier function
en-keyword=multiple chemical sensitivity
kn-keyword=multiple chemical sensitivity
en-keyword=formaldehyde
kn-keyword=formaldehyde
en-keyword=patch test
kn-keyword=patch test
en-keyword=prospective and follow-up study
kn-keyword=prospective and follow-up study
END