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Title Alternative ニンジン懸濁培養細胞の培養液に分泌される細胞外多糖成分
FullText URL 001_002_091_103.pdf
Author Konno, Haruyoshi| Yamasaki, Yoshiki| Katoh, Kenji|
Abstract The extracellular polysaccharides have been fractionated from the culture medium of carrot(Daucus carota)cell culrures by precipitation with ethanol and by chromatography on DEAE-Sepharose CL-6B DEAE-Trisacryl M ion-exchange and Bio-Gel A-1.5m gel-permeation.The sugar composition and molecular mass of purified neutral and acidic polymers were determined. The neutral and acidic polymers were treated with purified endo-Β-glucanase from Trichoderma viride and pectic depolymerases,such as endo-pectate lyase from Erwinia carotovora Er. and endo-polygalacturonase from Kluyveromyces fragilis, respectively. The "hairly"(ramified)regions of acidic polymer were sequentially treated with purified α-L-arabinofuranosidase and β-galactosidase from carrot cell cultures, and were further hydrolyzed with 50mM trifluoroacetic for 1 hr at 100℃. From these results, the extracellular polysaccharides secreted from carrot cell cultures are charactarized.
Keywords Carrot Cell suspension culture Cell wall Extracellular polysaccharides Pectic polymer
Publication Title Bulletin of the Research Institute For Bioresources, Okayama University
Published Date 1993
Volume volume1
Issue issue2
Start Page 91
End Page 103
ISSN 0916-930X
language English
File Version publisher
Author Murata, Yoshiyuki|
Published Date 1995-02-01
Publication Title 岡山大学農学部学術報告
Volume volume84
Issue issue1
Content Type Departmental Bulletin Paper
Author Yasui, Koichi|
Published Date 1995-02-01
Publication Title 岡山大学農学部学術報告
Volume volume84
Issue issue1
Content Type Departmental Bulletin Paper
Author Matsubara, Sachiko| Ino, Masato| Murakami, Kenji| Kamada, Mayumi| Ishihara, Izumi|
Published Date 1996-02-01
Publication Title 岡山大学農学部学術報告
Volume volume85
Issue issue1
Content Type Departmental Bulletin Paper
Author Yuhara, Masataka|
Published Date 1995-02-01
Publication Title 岡山大学農学部学術報告
Volume volume84
Issue issue1
Content Type Departmental Bulletin Paper
Author Nakamura, Reinosuke|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Takagi, Shigeaki|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Ning, Bo| Kubo, Yasutaka| Inaba, Akitsugu| Nakamura, Reinosuke|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Asaoka, Katsuhiro| Imai, Shunji| Okamoto, Goro| Hirano, Ken|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Goto, Tanjuro| Kageyama, Yoshihiro| Konishi, Kuniyoshi|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Goto, Tanjuro| Masaoka, Keishi| Kageyama, Yoshihiro| Konishi, Kuniyoshi|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Kawahara, Tomoharu| Toyoda, Kazuhiro| Kiba, Akinori| Ichinose, Yuki| Yamada, Tetsuji| Shiraishi, Tomonori|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Furukawa, Hirotaka| Sugio, Tsuyoshi| Nagasawa, Toru|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Murakami, Kenji| Nishioka, Junko| Matsubara, Sachiko|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Kawazu, Kazuyoshi| Ismail, Intan S.| Takahata, Kyouya| Ali, Abdul Manaf| Kanzaki, Hiroshi|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Matsubara, Sachiko| Yamamoto, Masatoyo| Jo, Man Hyun| Murakami, Kenji|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Toyoda, Kazuhiro| Koyama, Masashi| Mizukoshi, Rumi| Ichinose, Yuki| Yamada, Tetsuji| Shiraishi, Tomonori|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Nakauma, Makoto| Arami, Shin-ichiro| Hada, Megumi| Nakagawa, Ichiro| Murata, Yoshiyuki| Tada, Mikiro|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Takahata, Kyoya| Inouchi, saori| Monobe, Keiichi| Tada, Mikiro|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Inoue, Hiroyuki| Tamura, Takashi| Inagaki, Kenji| Tanaka, Hidehiko|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper