Basophil histamine release by anti-IgE in Subjects of chronic bronchitis and bronchial asthma

Histamine release from basophils induced by anti-IgE was studied in 8 patients with chronic bronchitis and 50 patients with bronchial asthma by analyzing doseresponse curves. As the result, there were no significant differences in maximum percent histamine release from basophils among three groups of healthy subjects (24.7± 14.2%), patients with chronic bronchitis (27.7±22.1%) and those with bronchial asthma (28.4±17.0%). In the patients with bronchial asthma, the maximum percent histamine release was higher in accordance with higher serum IgE levels, and low maximum percent release was observed in patients with intrinsic asthma (14.1±7.2%). Study of dose-response curves of anti-IgE-induced histamine release showed that a negative slope from E(2) to E(1) was observed in both healthy subjects and patients with chronic bronchitis. The majority of asthmatics with serum IgE levels of 501IU/ml or over showed a positive slope from E(2) to E(1).

気管支喘息50例，慢性気管支炎8例を対象に，抗ヒトIgE添加時の好塩基球からのヒスタミン遊離を全血法により行ない，その臨床的評価について検討を加えた。抗ヒトIgE海底時のMax　％　histamine releaseの平均は，健康人24.7±14.2％，慢性気管支炎27.7±22.1％，気管支喘息28.4±17.0％であり，3者間に有意の差はみられなかった。すなわち，抗ヒトIgE添加により健康人や慢性気管支炎患者の好塩基球からも有意のヒスタミン遊離が見られた。気管支喘息のなかでは，内因性喘息症例においてヒスタミン遊離（14.1±7.2％）の低い傾向が見られた。Dose-response curveの検討では，健康人，慢性気管支炎症例では全例E（2）からE（1）へかけてのnegative slopeを示した。気管支喘息症例では，血清IgE値500IU/ml以下の症例ではnegative slopeを示す症例が多く，一方501IU/ml以上の症例ではpositive slopeを示す症例がより多く見られた。