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Title Alternative Sexuality in plants, unveiled from genome evolution
FullText URL srfa_109_037_040.pdf
Author Akagi, Takashi|
Abstract Sexual polymorphism, a main strategy to maintain genetic diversity within a species, has long been a major focus in biology. Notwithstanding, in plants, evolution of sexual systems and mechanisms underlying these transitions have been little unveiled. We have elucidated the molecular mechanism of sex determination in persimmons (Diospyros spp.), where the Y-encoded smRNA gene OGI can repress the female-determining gene MeGI, and also in kiwifruits (Actinidia spp.), where the Y-encoded two sex determinants, Shy Girl and Friendly Boy, control gynoecium and androecium development, respectively. Although the molecular functions of these determinants are distinct, they have common evolutionary scenarios involving transitions of sexual systems. In persimmon, a recent genome triplication (hexaploidization) in cultivated persimmon (D. kaki) derived “flexible” sexuality via establishing epigenetic layers on the two sex determinants. On the other hand, an ancient Diospyros-specific paleo-genome duplication (paleo-tetraploidization) enabled neofunctionalization in the proto-MeGI, via positive selection, to establish a new function as a sex determinant. In kiwifruit, one of the two sex determinants, Shy Girl, was derived from neofunctionalization via Actinidia-specific duplication event. These findings exemplify how plant-specific numerous duplication events can drive flexible genetic material whose variation can be selected for development of new sexual systems.
Keywords Sex determination Sex chromosome Polyploidization Genome evolution Tree crops
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 37
End Page 40
ISSN 2186-7755
language Japanese
File Version publisher
Title Alternative Development of Effective Biological Treatment Process for Acid Mine Drainage
FullText URL srfa_109_029_036.pdf
Author Kamimura, Kazuo| Kanao, Tadayoshi|
Abstract Acid mine drainage (AMD) is acidic and generally enriched with iron, aluminum, sulfate and heavy metals, such as lead and cadmium. AMD is a growing problem of emerging concern that cause detrimental effects to the environment and living organisms. Yanahara mine in Misaki Town, Okayama, Japan, had mainly produced pyrite for sulfuric acid manufacture. Although it was closed in 1991, AMD is being generated from the mine now. A passive treatment based on the biological oxidation of ferrous iron is a promising strategy for AMD remediation. AMD from Yanahara mine is treated in a plant using iron-oxidizing bacteria, Acidithiobacillus ferrooxidans and Ferrovum spp. The AMD generation continues for several centuries with dramatic consequences on the receiving environments. Therefore, the development of sustainable and cost effective treatment process is required. A development of the effective biological treatment process with an iron oxidation reactor operated at pH 3.5 is described in this report. Economic aspects are also discussed.
Keywords acid mine drainage bioremediation iron-oxidizing bacteria microbial community Acidithiobacillus ferrooxidans
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 29
End Page 36
ISSN 2186-7755
language Japanese
File Version publisher
FullText URL JACS7_1_90.pdf
Author Hayakawa, Satoshi| Okamoto, Keigo| Yoshioka, Tomohiko|
Keywords Titanium substrate apatite deposition simulated body fluid parallel alignment titania layer
Published Date 2019-02-11
Publication Title Journal of Asian Ceramic Societies
Volume volume7
Issue issue1
Publisher Taylor & Francis
Start Page 90
End Page 100
ISSN 2187-0764
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s).
File Version publisher
DOI 10.1080/21870764.2019.1572690
Web of Science KeyUT 000459883500009
Related Url isVersionOf https://doi.org/10.1080/21870764.2019.1572690
FullText URL JACS20191699230.pdf
Author Liu, Xingzhu| Yoshioka, Tomohiko| Hayakawa, Satoshi|
Keywords Rutile nanorod arrays apatite rod density
Published Date 2019-12-02
Publication Title Journal of Asian Ceramic Societies
Publisher Taylor & Francis
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s).
File Version publisher
DOI 10.1080/21870764.2019.1699230
Web of Science KeyUT 000500730700001
Related Url isVersionOf https://doi.org/10.1080/21870764.2019.1699230
FullText URL AEZ54_4_389.pdf Fig.pdf
Author Sakakibara, Akira| Takebe, So| Ide, Toru| Hayakawa, Tohru|
Keywords Bacillus thuringiensis Cry46Ab toxin Planar lipid bilayer Single-channel analysis Calcein release assay
Published Date 2019-08-16
Publication Title Applied Entomology and Zoology
Volume volume54
Issue issue4
Publisher Springer
Start Page 389
End Page 398
ISSN 0003-6862
NCID AA00543238
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
DOI 10.1007/s13355-019-00635-z
Web of Science KeyUT 000503219100007
Related Url isVersionOf https://doi.org/10.1007/s13355-019-00635-z
Title Alternative Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells
FullText URL srfa_109_007_011.pdf
Author Saeki, Nozomu| Moriya, Hisao|
Abstract  Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency (fitness) produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy.
Keywords yeast 2 μ plasmid mathematical model
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 7
End Page 11
ISSN 2186-7755
language Japanese
File Version publisher
Title Alternative Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
FullText URL srfa_109_001_006.pdf
Author Kajiyama, Yuki| Mizobata, Satsuki| Akaji, Shusaku| Nemoto, Michiko| Tamura, Takashi| Inagaki, Kenji|
Abstract  Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
Keywords glycine oxidase Marinomonas mediterranea cysteine tryptophilquinone recombinant expression enzymatic glycine assay
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 1
End Page 6
ISSN 2186-7755
language Japanese
File Version publisher
FullText URL fulltext.pdf Graphical_Abstract.pptx Figure1-6.pdf Supp_Info.pdf
Author Kanaya, Nobuhiko| Kuroda, Shinji| Kakiuchi, Yoshihiko| Kumon, Kento| Tsumura, Tomoko| Hashimoto, Masashi| Morihiro, Toshiaki| Kubota, Tetsushi| Aoyama, Katsuyuki| Kikuchi, Satoru| Nishizaki, Masahiko| Kagawa, Shunsuke| Tazawa, Hiroshi| Mizuguchi, Hiroyuki| Urata, Yasuo| Fujiwara, Toshiyoshi|
Keywords immune checkpoint programmed death-1 oncolytic adenovirus combined immunotherapy immunogenic cell death tumor infiltrating lymphocytes CD8 abscopal effect
Published Date 2020-01-10
Publication Title Molecular Therapy
Volume volume28
Issue issue3
Publisher Cell Press
Start Page 794
End Page 804
ISSN 15250016
NCID AA11475060
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 31991110
DOI 10.1016/j.ymthe.2020.01.003
Web of Science KeyUT 000518887200012
Related Url isVersionOf https://doi.org/10.1016/j.ymthe.2020.01.003
FullText URL fulltext.pdf
Author Ueki, Yushi| Saito, Ken| Iioka, Hidekazu| Sakamoto, Izumi| Kanda, Yasuhiro| Sakaguchi, Masakiyo| Horii, Arata| Kondo, Eisaku|
Keywords Cancer Molecular Biology
Published Date 2020-01-18
Publication Title iScience
Volume volume23
Issue issue2
Publisher Cell Press
Start Page 100850
ISSN 25890042
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2020 The Author(s).
File Version publisher
PubMed ID 32058962
DOI 10.1016/j.isci.2020.100850
Web of Science KeyUT 000518637100047
Related Url isVersionOf https://doi.org/10.1016/j.isci.2020.100850
FullText URL fulltext.pdf
Author Suga, Michihiro| Shimada, Atsuhiro| Akita, Fusamichi| Shen, Jian-Ren| Tosha, Takehiko| Sugimoto, Hiroshi|
Keywords Heme Metalloproteins Proton pump Radiation damage Serial femtosecond crystallography X-ray free-electron laser
Published Date 2020-02-29
Publication Title Biochimica et Biophysica Acta (BBA) - General Subjects
Volume volume1864
Issue issue2
Publisher SI
Start Page 129466
ISSN 03044165
NCID AA00564679
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s). Published by Elsevier B.V.
File Version publisher
PubMed ID 31678142
DOI 10.1016/j.bbagen.2019.129466
Web of Science KeyUT 000508740100002
Related Url isVersionOf https://doi.org/10.1016/j.bbagen.2019.129466
FullText URL srfa_109_contents.pdf
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
ISSN 2186-7755
language Japanese
File Version publisher
FullText URL srfa_109_cover.pdf
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
ISSN 2186-7755
language Japanese
File Version publisher
FullText URL PO14_5_217517.pdf
Author Ryuno, Hiroki| Nigo, Fuki| Naguro, Isao| Sekimizu, Kazuhisa| Kaito, Chikara|
Published Date 2019-05-30
Publication Title PLoS ONE
Volume volume14
Issue issue5
Publisher Public Library of Science
Start Page e0217517
ISSN 1932-6203
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders Copyright: © 2019 Ryuno et al.
File Version publisher
PubMed ID 31145754
DOI 10.1371/journal.pone.0217517
Web of Science KeyUT 000469425500020
Related Url isVersionOf https://doi.org/10.1371/journal.pone.0217517
FullText URL JBR_2019_1.pdf Table.pdf Fig.pdf Fig_Supple.xps
Author Sekiguchi, Manabu| Inoue, Kotaro| Yang, Tian| Luo, Dong-Gen| Yoshii, Taishi|
Keywords Drosophila clock neuron split-GAL4
Published Date 2019-12-19
Publication Title Journal of Biological Rhythms
Publisher SAGE Publications
ISSN 0748-7304
NCID AA10667882
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 31856635
DOI 10.1177/0748730419895154
Web of Science KeyUT 000504544500001
Related Url isVersionOf https://doi.org/10.1177/0748730419895154
FullText URL psychogeriatrics19_6_566.pdf table.pdf
Author Takenoshita, Shintaro| Terada, Seishi| Oshima, Etsuko| Yamaguchi, Megumi| Hayashi, Satoshi| Hinotsu, Kenji| Esumi, Satoru| Shinya, Takayoshi| Yamada, Norihito|
Keywords depression elderly Lewy body disease metaiodobenzylguanidine somatic anxiety
Published Date 2019-02-27
Publication Title Psychogeriatrics
Volume volume19
Issue issue6
Publisher Wiley
Start Page 566
End Page 573
ISSN 1346-3500
NCID AA11919302
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 30809892
DOI 10.1111/psyg.12439
Web of Science KeyUT 000495852300005
Related Url isVersionOf https://doi.org/10.1111/psyg.12439
FullText URL NMB43_6_372.pdf
Author Matsuura, Yuki| Ueda, Masashi| Higaki, Yusuke| Watanabe, Keiko| Habara, Shogo| Kamino, Shinichiro| Saji, Hideo| Enomoto, Shuichi|
Keywords 5-[(123)I]iodo-3-(2(S)-azetidinylmethoxy)pyridine ([(123)I]5IA) Alzheimer's disease Mouse Nicotinic acetylcholine receptor (nAChR) Single-photon emission computed tomography (SPECT) Tg2576
Published Date 2016-06
Publication Title Nuclear Medicine and Biology
Volume volume43
Issue issue6
Publisher Elsevier Science
Start Page 372
End Page 378
ISSN 0969-8051
NCID AA10864894
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 27260778
DOI 10.1016/j.nucmedbio.2016.02.007
Web of Science KeyUT 000378011300007
Related Url isVersionOf https://doi.org/10.1016/j.nucmedbio.2016.02.007
FullText URL MIB21_3_519.pdf
Author Matsuura, Yuki| Ueda, Masashi| Higaki, Yusuke| Sano, Kohei| Saji, Hideo| Enomoto, Shuichi|
Keywords Alzheimer's disease Nicotinic acetylcholine receptors 2-Deoxy-2-[F-18]fluoro-D-glucose ([F-18]FDG) 5-[I-123]Iodo-3-[2(S)-azetidinylmethoxy]pyridine ([I-123]5IA) APP PS2 mice
Published Date 2018-07-25
Publication Title Molecular Imaging and Biology
Volume volume21
Issue issue3
Publisher Springer
Start Page 519
End Page 528
ISSN 1536-1632
NCID AA1170000X
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 30047036
DOI 10.1007/s11307-018-1253-4
Web of Science KeyUT 000468363800015
Related Url isVersionOf https://doi.org/10.1007/s11307-018-1253-4
FullText URL CC55_27_3923.pdf
Author Niwa, Yutaro| Miyake, Mayu| Hayakawa, Ichiro| Sakakura, Akira|
Published Date 2019-04-07
Publication Title Chemical Communications
Volume volume55
Issue issue27
Publisher Royal Society of Chemistry
Start Page 3923
End Page 3926
ISSN 1359-7345
NCID AA11071130
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 30919859
DOI 10.1039/c9cc01159e
Web of Science KeyUT 000463952600013
Related Url isVersionOf https://doi.org/10.1039/c9cc01159e
FullText URL SR9_1_4722.pdf
Author Nakatani, Takahiro| Yasui, Norihisa| Tamura, Issei| Yamashita, Atsuko|
Published Date 2019-3-18
Publication Title Scientific Reports
Volume volume9
Publisher Nature Publishing Group
Start Page 4722
ISSN 2045-2322
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version publisher
PubMed ID 30886277
DOI 10.1038/s41598-019-41309-8
Web of Science KeyUT 000461417500005
Related Url isVersionOf https://doi.org/10.1038/s41598-019-41309-8
FullText URL SR9_1_8041.pdf
Author Yoshioka, Yohsuke| Yamachika, Eiki| Nakanishi, Makoto| Ninomiya, Tadashi| Akashi, Sho| Kondo, Sei| Moritani, Norifumi| Kobayashi, Yasuhiro| Fujii, Tatsuo| Iida, Seiji|
Published Date 2019-5-29
Publication Title Scientific Reports
Volume volume9
Publisher Nature Publishing Group
Start Page 8041
ISSN 2045-2322
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2019
File Version publisher
PubMed ID 31142769
DOI 10.1038/s41598-019-44389-8
Web of Science KeyUT 000469319300003
Related Url isVersionOf https://doi.org/10.1038/s41598-019-44389-8