start-ver=1.4 cd-journal=joma no-vol=234 cd-vols= no-issue= article-no= start-page=125 end-page=132 dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20250301 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mitochondrial content and mtDNA copy number in spermatozoa and penetrability into oocytes en-subtitle= kn-subtitle= en-abstract= kn-abstract=The current narrative review aims to summarize the relation of mitochondrial content (MC) and mitochondrial DNA copy number (MDCN) in spermatozoa with sperm penetrability, and to discuss the various determining factors during the process of spermatogenesis in mammals. There are many potential factors associated with the quantitative alteration of MC and MDCN in male gametes from spermatogenesis to ejaculation. Particularly, spermatogenesis may be the first step to jointly contribute to an incomplete reduction of MC and MDCN in spermatozoon. It appears to be now quite clear that some abnormalities during spermatogenesis and oxidative stress are the main factors highly associated with the quantitative change of MC and MDCN in spermatozoa, consequently affecting sperm quality and their penetrability into oocytes. Currently, a series of proteins contributing to form sperm midpiece during spermatogenesis and cytoplasmic elimination during spermiation have been currently identified. The present review provides insight into how these factors interact with sperm MC and MDCN, and handholds to gain a better understanding of their roles. This review also highlights the uniqueness of normal fertile spermatozoa which have relatively lower MC and MDCN, but have mitochondria that function completely in multiple pivotal physiological pathways. en-copyright= kn-copyright= en-aut-name=NguyenHai Thanh en-aut-sei=Nguyen en-aut-mei=Hai Thanh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DoSon Quang en-aut-sei=Do en-aut-mei=Son Quang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WakaiTakuya en-aut-sei=Wakai en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FunahashiHiroaki en-aut-sei=Funahashi en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Animal Science, Okayama University kn-affil= affil-num=2 en-affil=Department of Animal Science, Okayama University kn-affil= affil-num=3 en-affil=Department of Animal Science, Okayama University kn-affil= affil-num=4 en-affil=Department of Animal Science, Okayama University kn-affil= en-keyword=Spermatozoa kn-keyword=Spermatozoa en-keyword=Mitochondria kn-keyword=Mitochondria en-keyword=Mitochondrial DNA kn-keyword=Mitochondrial DNA en-keyword=Penetrability kn-keyword=Penetrability en-keyword=Spermatogenesis kn-keyword=Spermatogenesis END start-ver=1.4 cd-journal=joma no-vol=228 cd-vols= no-issue= article-no= start-page=30 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20241015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Exogenous expression of PGC-1α during in vitro maturation impairs the developmental competence of porcine oocytes en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objectives of the current study were to examine the effects of exogenous expression of PGC-1α, which is a transcription factor responsive for controlling mitochondrial DNA (mtDNA) replication, mitochondria quantity control, mitochondrial biogenesis, and reactive oxygen species (ROS) maintenance, in porcine oocytes during in-vitro maturation (IVM) on the developmental competence, as well as mitochondrial quantity and function. Exogenous over-expression of PGC-1α by injection of the mRNA construct into oocytes 20 h after the start of IVM culture significantly increased the copy number of mtDNA in the oocytes, but reduced the incidences of oocytes matured to the metaphase-II stage after the IVM culture for totally 44 h and completely suppressed the early development in vitro to the blastocyst stage following parthenogenetic activation. The exogenous expression of PGC-1α also significantly induced spindle defects and chromosome misalignments. Furthermore, markedly higher ROS levels were observed in the PGC-1α-overexpressed mature oocytes, whereas mRNA level of SOD1, encoded for a ROS scavenging enzyme, was decreased. These results conclude that forced expression of PGC-1α successfully increase mtDNA copy number but led to increased ROS production, evidently by downregulation of SOD1 gene expression, inducement of spindle aberration/chromosomal misalignment, and consequently reduction in the meiotic and developmental competences of porcine oocytes. en-copyright= kn-copyright= en-aut-name=DoSon Quang en-aut-sei=Do en-aut-mei=Son Quang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NguyenHai Thanh en-aut-sei=Nguyen en-aut-mei=Hai Thanh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WakaiTakuya en-aut-sei=Wakai en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FunahashiHiroaki en-aut-sei=Funahashi en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=Porcine kn-keyword=Porcine en-keyword=Mitochondria kn-keyword=Mitochondria en-keyword=Oocytes kn-keyword=Oocytes en-keyword=PGC-1 alpha kn-keyword=PGC-1 alpha en-keyword=In vitro maturation kn-keyword=In vitro maturation END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue=1 article-no= start-page=42 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20241126 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Genotypes and phenotypes of neurofibromatosis type 1 patients in Japan: A Hereditary Tumor Cohort Study en-subtitle= kn-subtitle= en-abstract= kn-abstract=Neurofibromatosis type 1 (NF1) presents with a broad spectrum of clinical manifestations, including an increased risk of tumor development and hypertension. Comprehensive data on genotype‒phenotype correlations in patients with NF1 are limited. Therefore, in this study, we aimed to elucidate the detailed genetic and clinical characteristics of NF1 in a hereditary tumor cohort. We performed sequencing and copy number assays in a clinical laboratory and analyzed the clinical data of 44 patients with suspected NF1. Germline pathogenic variants were detected in 36 patients (81.8%), and 20.7% of the variants were novel. Notably, 40.0% of adult patients presented with malignancies; female breast cancer occurred in 20.0% of patients, which was a higher rate than that previously reported. Hypertension was observed in 30.6% of the adult patients, with one patient experiencing sudden death and another developing pheochromocytoma. Three patients with large deletions in NF1 exhibited prominent cutaneous, skeletal, and neurological manifestations. These results highlight the importance of regular surveillance, particularly for patients with malignancies and hypertension. Our findings provide valuable insights for genetic counseling and clinical management, highlighting the multiple health risks associated with NF1 and the need for comprehensive and multidisciplinary care. en-copyright= kn-copyright= en-aut-name=FutagawaMashu en-aut-sei=Futagawa en-aut-mei=Mashu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkazakiTetsuya en-aut-sei=Okazaki en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakataEiji en-aut-sei=Nakata en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FukanoChika en-aut-sei=Fukano en-aut-mei=Chika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OsumiRisa en-aut-sei=Osumi en-aut-mei=Risa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KatoFumino en-aut-sei=Kato en-aut-mei=Fumino kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=UrakawaYusaku en-aut-sei=Urakawa en-aut-mei=Yusaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=YamamotoHideki en-aut-sei=Yamamoto en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=HirasawaAkira en-aut-sei=Hirasawa en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Orthopedic Surgery, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Clinical Genetics and Genomic Medicine, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Clinical Genetics and Genomic Medicine, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Genetic Medicine, School of Medicine, Fujita Health University kn-affil= affil-num=8 en-affil=Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Orthopedic Surgery, Okayama University Hospital kn-affil= affil-num=10 en-affil=Department of Clinical Genomic Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=免疫不全/調節異常に起因する古典的ホジキンリンパ腫における9p24.1のコピー数解析 kn-title=Copy Number Analysis of 9p24.1 in Classic Hodgkin Lymphoma Arising in Immune Deficiency/Dysregulation en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OHSAWAKumiko en-aut-sei=OHSAWA en-aut-mei=Kumiko kn-aut-name=大澤久美子 kn-aut-sei=大澤 kn-aut-mei=久美子 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Health Sciences, Okayama University kn-affil=岡山大学大学院保健学研究科 END start-ver=1.4 cd-journal=joma no-vol=78 cd-vols= no-issue=5 article-no= start-page=371 end-page=376 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=202410 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Phenotypic and Genetic Characteristics of Carbapenemase-Producing Enterobacterales Isolates at Okayama University Hospital en-subtitle= kn-subtitle= en-abstract= kn-abstract=Spread of carbapenemase-producing Enterobacterales (CPE) is an ongoing public health issue worldwide, including in Japan. In this study, we investigated the phenotypic and genetic characteristics of CPE isolates at Okayama University Hospital over the 5 years (2013-2018) prior to the outbreak of the 2019 coronavirus pandemic. Of 24 carbapenem-resistant Enterobacterales isolated during the study period, we identified 8 CPE isolates harboring blaIMP-1 (5 isolates) and blaIMP-6 genes (3 isolates). Bacterial species and carbapenem susceptibility patterns exhibited diversity. Minimum inhibitory concentrations (MICs) of meropenem were generally higher than those of imipenem and biapenem. Results of pulsed-field gel electrophoresis demonstrated that neither clonal nor plasmid-mediated outbreaks of blaIMP-harboring CPE isolates have developed at our hospital. One Klebsiella oxytoca isolate showed a high MIC (128 μg/mL) of meropenem, which could be explained by the high plasmid copy number. Subsequent analysis of this isolate may elucidate the intricacies of carbapenem resistance profiles among CPE isolates. Collectively, our findings underscore the necessity for ongoing genetic surveillance of CPE, complemented by tailored approaches for infection prevention and control. en-copyright= kn-copyright= en-aut-name=GotohKazuyoshi en-aut-sei=Gotoh en-aut-mei=Kazuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyoshiMakoto en-aut-sei=Miyoshi en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=I Putu Bayu Mayura en-aut-sei=I Putu Bayu Mayura en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TsujiShuma en-aut-sei=Tsuji en-aut-mei=Shuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IioKoji en-aut-sei=Iio en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=FukushimaShinnosuke en-aut-sei=Fukushima en-aut-mei=Shinnosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MatsushitaOsamu en-aut-sei=Matsushita en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HagiyaHideharu en-aut-sei=Hagiya en-aut-mei=Hideharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Medical Laboratory Science, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=3 en-affil=Department of Clinical Microbiology, Faculty of Medicine, Udayana University kn-affil= affil-num=4 en-affil=Department of Medical Laboratory Science, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=5 en-affil=Microbiology Division, Clinical Laboratory, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Infectious Diseases, Okayama University Hospital kn-affil= en-keyword=antimicrobial resistance kn-keyword=antimicrobial resistance en-keyword=carbapenemase-producing enterobacterales kn-keyword=carbapenemase-producing enterobacterales en-keyword=carbapenemase-resistant enterobacterales kn-keyword=carbapenemase-resistant enterobacterales en-keyword=Silent pandemic kn-keyword=Silent pandemic en-keyword=whole genome sequence kn-keyword=whole genome sequence END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue= article-no= start-page=1339958 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240829 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Illumina-based transcriptomic analysis of the fast-growing leguminous tree Acacia crassicarpa: functional gene annotation and identification of novel SSR-markers en-subtitle= kn-subtitle= en-abstract= kn-abstract=Acacia crassicarpa is a fast-growing leguminous tree that is widely cultivated in tropical areas such as Indonesia, Malaysia, Australia, and southern China. This tree has versatile utility in timber, furniture, and pulp production. Illumina sequencing of A. crassicarpa was conducted, and the raw data of 124,410,892 reads were filtered and assembled de novo into 93,317 unigenes, with a total of 84,411,793 bases. Blast2GO annotation, Benchmark Universal Single-Copy Ortholog evaluation, and GO-term classification produced a catalogue of unigenes for studying primary metabolism, phytohormone signaling, and transcription factors. Massive transcriptomic analysis has identified microsatellites composed of simple sequence repeat (SSR) loci representing di-, tri-, and tetranucleotide repeat units in the predicted open reading frames. Polymorphism was induced by PCR amplification of microsatellite loci located in several genes encoding auxin response factors and other transcription factors, which successfully distinguished 16 local trees of A. crassicarpa tested, representing potentially exploitable molecular markers for efficient tree breeding for plantation and biomass exploitation. en-copyright= kn-copyright= en-aut-name=IshioShougo en-aut-sei=Ishio en-aut-mei=Shougo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KusunokiKazutaka en-aut-sei=Kusunoki en-aut-mei=Kazutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NemotoMichiko en-aut-sei=Nemoto en-aut-mei=Michiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KanaoTadayoshi en-aut-sei=Kanao en-aut-mei=Tadayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Tsukuba Research Institute, Sumitomo Forestry Co. Ltd. kn-affil= affil-num=2 en-affil=Tsukuba Research Institute, Sumitomo Forestry Co. Ltd. kn-affil= affil-num=3 en-affil=Graduate School of Environment, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Environment, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=5 en-affil=Institute of Global Human Resource Development, Okayama University kn-affil= en-keyword=Acacia crassicarpa kn-keyword=Acacia crassicarpa en-keyword= illumina sequencing kn-keyword= illumina sequencing en-keyword= polymorphism kn-keyword= polymorphism en-keyword= auxin response factor kn-keyword= auxin response factor en-keyword= lignin kn-keyword= lignin END start-ver=1.4 cd-journal=joma no-vol=16 cd-vols= no-issue=7 article-no= start-page=1298 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Copy Number Analysis of 9p24.1 in Classic Hodgkin Lymphoma Arising in Immune Deficiency/Dysregulation en-subtitle= kn-subtitle= en-abstract= kn-abstract=A subset of patients with rheumatoid arthritis receiving methotrexate develop immune deficiencies and dysregulation-associated lymphoproliferative disorders. Patients with these disorders often exhibit spontaneous regression after MTX withdrawal; however, chemotherapeutic intervention is frequently required in patients with classic Hodgkin lymphoma arising in immune deficiency/dysregulation. In this study, we examined PD-L1 expression levels and 9p24.1 copy number alterations in 27 patients with classic Hodgkin lymphoma arising from immune deficiency/dysregulation. All patients demonstrated PD-L1 protein expression and harbored 9p24.1 copy number alterations on the tumor cells. When comparing clinicopathological data and associations with 9p24.1 copy number features, the copy gain group showed a significantly higher incidence of extranodal lesions and clinical stages than the amplification group. Notably, all cases in the amplification group had latency type II, while 6/8 (75%) in the copy gain group had latency type II, and 2/8 (25%) had latency type I. Thus, a subset of the copy-gain group demonstrated more extensive extranodal lesions and higher clinical stages. This finding speculates the presence of a genetically distinct subgroup within the group of patients who develop immune deficiencies and dysregulation-associated lymphoproliferative disorders, which may explain certain characteristic features. en-copyright= kn-copyright= en-aut-name=OhsawaKumiko en-aut-sei=Ohsawa en-aut-mei=Kumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MomoseShuji en-aut-sei=Momose en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishikoriAsami en-aut-sei=Nishikori en-aut-mei=Asami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishimuraMidori Filiz en-aut-sei=Nishimura en-aut-mei=Midori Filiz kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=GionYuka en-aut-sei=Gion en-aut-mei=Yuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SawadaKeisuke en-aut-sei=Sawada en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HigashiMorihiro en-aut-sei=Higashi en-aut-mei=Morihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TokuhiraMichihide en-aut-sei=Tokuhira en-aut-mei=Michihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TamaruJun-Ichi en-aut-sei=Tamaru en-aut-mei=Jun-Ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SatoYasuharu en-aut-sei=Sato en-aut-mei=Yasuharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Molecular Hematopathology, Okayama University Graduate School of Health Sciences kn-affil= affil-num=2 en-affil=Department of Pathology, Saitama Medical Center, Saitama Medical University kn-affil= affil-num=3 en-affil=Department of Molecular Hematopathology, Okayama University Graduate School of Health Sciences kn-affil= affil-num=4 en-affil=Department of Molecular Hematopathology, Okayama University Graduate School of Health Sciences kn-affil= affil-num=5 en-affil=Department of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences kn-affil= affil-num=6 en-affil=Department of Pathology, Saitama Medical Center, Saitama Medical University kn-affil= affil-num=7 en-affil=Department of Pathology, Saitama Medical Center, Saitama Medical University kn-affil= affil-num=8 en-affil=Department of Hematology, Japan Community Health Care Organization Saitama Medical Center kn-affil= affil-num=9 en-affil=Department of Pathology, Saitama Medical Center, Saitama Medical University kn-affil= affil-num=10 en-affil=Department of Molecular Hematopathology, Okayama University Graduate School of Health Sciences kn-affil= en-keyword=classic Hodgkin lymphoma kn-keyword=classic Hodgkin lymphoma en-keyword=methotrexate kn-keyword=methotrexate en-keyword=immunodeficiency kn-keyword=immunodeficiency en-keyword=programmed cell death-ligand 1 kn-keyword=programmed cell death-ligand 1 en-keyword=rheumatoid arthritis kn-keyword=rheumatoid arthritis END start-ver=1.4 cd-journal=joma no-vol=14 cd-vols= no-issue=1 article-no= start-page=4953 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240229 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Long-term monitoring of gastric mucosa-associated lymphoid tissue lymphoma in patients with extra copies of the MALT1 gene en-subtitle= kn-subtitle= en-abstract= kn-abstract=The objective of this study was to clarify the long-term prognosis of patients with gastric mucosa-associated lymphoid tissue (MALT) lymphoma with additional copies of MALT1. In this multicenter retrospective study, we enrolled 145 patients with gastric MALT lymphoma who underwent fluorescence in situ hybridization (FISH) analysis to detect t(11;18) translocation. The patient cohort was divided into three groups: Group A (n = 87), comprising individuals devoid of the t(11;18) translocation or extra MALT1 copies; Group B (n = 27), encompassing patients characterized by the presence of the t(11;18) translocation; and Group C (n = 31), including patients with extra MALT1 copies. The clinical outcomes in each cohort were collected. Over the course of a mean follow-up of 8.5 ± 4.2 years, one patient died of progressive MALT lymphoma, while 15 patients died due to etiologies unrelated to lymphoma. The progression or relapse of MALT lymphoma was observed in 11 patients: three in Group A, two in Group B, and six in Group C. In Groups A, B, and C, the 10-year overall survival rates were 82.5%, 93.8%, and 86.4%, respectively, and the 10-year event-free survival rates were 96.1%, 96.0%, and 82.9%, respectively. The event-free survival rate in Group C was significantly lower than that in Group A. However, no differences were observed in the 10-year event-free survival rates among individuals limited to stage I or II1 disease (equivalent to excluding patients with stage IV disease in this study, as there were no patients with stage II2), with rates of 98.6%, 95.8%, and 92.3% for Groups A, B, and C, respectively. In conclusion, the presence of extra copies of MALT1 was identified as an inferior prognostic determinant of event-free survival. Consequently, trisomy/tetrasomy 18 may serve as an indicator of progression and refractoriness to therapeutic intervention in patients with gastric MALT lymphoma, particularly stage IV gastric MALT lymphoma. en-copyright= kn-copyright= en-aut-name=IwamuroMasaya en-aut-sei=Iwamuro en-aut-mei=Masaya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakenakaRyuta en-aut-sei=Takenaka en-aut-mei=Ryuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyaharaKoji en-aut-sei=Miyahara en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkanoueShotaro en-aut-sei=Okanoue en-aut-mei=Shotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YoshiokaMasao en-aut-sei=Yoshioka en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SakaguchiChihiro en-aut-sei=Sakaguchi en-aut-mei=Chihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YamamotoKumiko en-aut-sei=Yamamoto en-aut-mei=Kumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KawaiYoshinari en-aut-sei=Kawai en-aut-mei=Yoshinari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ToyokawaTatsuya en-aut-sei=Toyokawa en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TanakaTakehiro en-aut-sei=Tanaka en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=OtsukaMotoyuki en-aut-sei=Otsuka en-aut-mei=Motoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil= Department of Internal Medicine, Tsuyama Chuo Hospital kn-affil= affil-num=3 en-affil=Department of Internal Medicine, Hiroshima City Hospital kn-affil= affil-num=4 en-affil=Department of Gastroenterology, Mitoyo General Hospital kn-affil= affil-num=5 en-affil=Department of Internal Medicine, Okayama Saiseikai General Hospital kn-affil= affil-num=6 en-affil=Department of Gastroenterology, Shikoku Cancer Center kn-affil= affil-num=7 en-affil=Department of Gastroenterology, Kagawa Prefectural Central Hospital kn-affil= affil-num=8 en-affil=Department of Gastroenterology, Onomichi Municipal Hospital kn-affil= affil-num=9 en-affil=Department of Gastroenterology, Fukuyama Medical Center kn-affil= affil-num=10 en-affil=Department of Pathology, Okayama University Hospital kn-affil= affil-num=11 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue kn-keyword=Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue en-keyword=Gastric neoplasms kn-keyword=Gastric neoplasms en-keyword=Esophagogastroduodenoscopy kn-keyword=Esophagogastroduodenoscopy en-keyword=t(11;18) translocation, kn-keyword=t(11;18) translocation, en-keyword=Trisomy 18 kn-keyword=Trisomy 18 END start-ver=1.4 cd-journal=joma no-vol=24 cd-vols= no-issue=18 article-no= start-page=14128 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20230915 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders en-subtitle= kn-subtitle= en-abstract= kn-abstract=The mammalian cerebral cortex undergoes a strictly regulated developmental process. Detailed in situ visualizations, imaging of these dynamic processes, and in vivo functional gene studies significantly enhance our understanding of brain development and related disorders. This review introduces basic techniques and recent advancements in in vivo electroporation for investigating the molecular mechanisms underlying cerebral diseases. In utero electroporation (IUE) is extensively used to visualize and modify these processes, including the forced expression of pathological mutants in human diseases; thus, this method can be used to establish animal disease models. The advent of advanced techniques, such as genome editing, including de novo knockout, knock-in, epigenetic editing, and spatiotemporal gene regulation, has further expanded our list of investigative tools. These tools include the iON expression switch for the precise control of timing and copy numbers of exogenous genes and TEMPO for investigating the temporal effects of genes. We also introduce the iGONAD method, an improved genome editing via oviductal nucleic acid delivery approach, as a novel genome-editing technique that has accelerated brain development exploration. These advanced in vivo electroporation methods are expected to provide valuable insights into pathological conditions associated with human brain disorders. en-copyright= kn-copyright= en-aut-name=YangChen en-aut-sei=Yang en-aut-mei=Chen kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShitamukaiAtsunori en-aut-sei=Shitamukai en-aut-mei=Atsunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YangShucai en-aut-sei=Yang en-aut-mei=Shucai kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KawaguchiAyano en-aut-sei=Kawaguchi en-aut-mei=Ayano kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Human Anatomy and Histology and Embryology, School of Basic Medicine, Harbin Medical University kn-affil= affil-num=4 en-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences kn-affil= en-keyword=in vivo electroporation kn-keyword=in vivo electroporation en-keyword=in utero electroporation kn-keyword=in utero electroporation en-keyword=genome editing kn-keyword=genome editing en-keyword=IUE kn-keyword=IUE en-keyword=iON kn-keyword=iON en-keyword=TEMPO kn-keyword=TEMPO en-keyword=iGONAD kn-keyword=iGONAD END start-ver=1.4 cd-journal=joma no-vol=210 cd-vols= no-issue= article-no= start-page=154 end-page=161 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20231015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Negative correlations of mitochondrial DNA copy number in commercial frozen bull spermatozoa with the motility parameters after thawing en-subtitle= kn-subtitle= en-abstract= kn-abstract=The purpose of the current study was to investigate the relationship between mitochondrial content of commercial frozen-thawed bull spermatozoa and motility. Firstly, mitochondrial DNA copy number per spermatozoon (MDCN), mitochondrial content (MC), the percentage of spermatozoa with high mitochondrial membrane potential (HMMP), intracellular reactive oxygen species (ROS) and motility parameters of frozen-thawed spermatozoa derived from five bulls were determined by using qPCR, flow cytometry and CASA, respectively, and analyzed the relationships. Results showed that all parameters examined, including MDCN, MC, HMMP, ROS and motility indicators, significantly differed among frozen spermatozoa from different bulls. Both MDCN and MC were negatively correlated with HMMP and motility indicators, but positively with ROS, of course, whereas there was a highly positive relationship between MDCN and MC. Secondly, when MDCN and MC were examined in frozen spermatozoa prepared at different points in the lives of four bulls, those did not correlate overall throughout their lives (1.3–14.3 years old), but did correlate significantly in two sires. From these results, we conclude that MDCN and MC of frozen spermatozoa differ among sires, and are negatively correlated with HMMP and sperm motility parameters, probably due to mitochondrial oxidative stress resulted in the presence of ROS, demonstrating that these appear to be useful markers to assess sires’ spermatozoa. It should be noted that the MDCN and MC of bull spermatozoa may not vary overall with the age of the sire, whereas those changes with age in some individuals and may affect sperm motility. en-copyright= kn-copyright= en-aut-name=NguyenHai Thanh en-aut-sei=Nguyen en-aut-mei=Hai Thanh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DoSon Quang en-aut-sei=Do en-aut-mei=Son Quang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiHiroshi en-aut-sei=Kobayashi en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WakaiTakuya en-aut-sei=Wakai en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FunahashiHiroaki en-aut-sei=Funahashi en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Okayama Prefectural Center for Animal Husbandry and Research kn-affil= affil-num=4 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=Spermatozoa kn-keyword=Spermatozoa en-keyword=Bulls kn-keyword=Bulls en-keyword=Mitochondrial content kn-keyword=Mitochondrial content en-keyword=Motility kn-keyword=Motility en-keyword=Frozen semen kn-keyword=Frozen semen END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=5 article-no= start-page=545 end-page=552 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202310 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Endoscopic Manifestations and Clinical Characteristics of Localized Gastric Light-Chain Amyloidosis en-subtitle= kn-subtitle= en-abstract= kn-abstract=To determine the endoscopic and clinical features of localized gastric amyloid light-chain (AL) amyloidosis, we retrospectively examined the characteristics of nine patients (eight men and one woman) encountered by the hospitals in our network. Lesions were predominantly flat and depressed with surface vascular dilatation (n=5); others were characterized by subepithelial lesions (n=2), mucosal color change (n=1), and a mass-like morphology with swollen mucosal folds (n=1). Colonoscopy (n=7), video capsule enteroscopy (n=2), serum (n=5) and urine immunoelectrophoresis (n=4), and bone marrow examination (n=3) were performed to exclude involvement of organs other than the stomach. As treatment for gastric lesions of AL amyloidosis, one patient each underwent endoscopic submucosal dissection (n=1) and argon plasma coagulation (n=1), while the remaining seven patients underwent no specific treatment. During a mean follow-up of 4.2 years, one patient died 3.2 years after diagnosis, but the cause of death, which occurred in another hospital, was unknown. The remaining eight patients were alive at the last visit. In conclusion, although localized gastric AL amyloidosis can show various macroscopic features on esophagogastroduodenoscopy, flat, depressed lesions with vascular dilatation on the surface are predominant. en-copyright= kn-copyright= en-aut-name=IwamuroMasaya en-aut-sei=Iwamuro en-aut-mei=Masaya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaShouichi en-aut-sei=Tanaka en-aut-mei=Shouichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ToyokawaTatsuya en-aut-sei=Toyokawa en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishimuraMamoru en-aut-sei=Nishimura en-aut-mei=Mamoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsuzukiTakao en-aut-sei=Tsuzuki en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyaharaKoji en-aut-sei=Miyahara en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NegishiShin en-aut-sei=Negishi en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhyaShogen en-aut-sei=Ohya en-aut-mei=Shogen kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TanakaTakehiro en-aut-sei=Tanaka en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OtsukaMotoyuki en-aut-sei=Otsuka en-aut-mei=Motoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterology, National Hospital Organization Iwakuni Clinical Center kn-affil= affil-num=3 en-affil=Department of Gastroenterology, National Hospital Organization Fukuyama Medical Center kn-affil= affil-num=4 en-affil=Department of Internal Medicine, Okayama City Hospital kn-affil= affil-num=5 en-affil=Department of Internal Medicine, Japanese Red Cross Society Himeji Hospital kn-affil= affil-num=6 en-affil=Department of Internal Medicine, Hiroshima City Hiroshima Citizens Hospital kn-affil= affil-num=7 en-affil=Department of Gastroenterology, Kagawa Prefectural Central Hospital kn-affil= affil-num=8 en-affil=Kawaguchi Medical Clinic kn-affil= affil-num=9 en-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=esophagogastroduodenoscopy kn-keyword=esophagogastroduodenoscopy en-keyword=gastric lesion kn-keyword=gastric lesion en-keyword=amyloidosis kn-keyword=amyloidosis en-keyword=light chain kn-keyword=light chain END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20230324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=金重陶陽の表現様式と影響：写し、偶然性、不完全さに関する考察を通じて kn-title=Kaneshige Tōyō's Style and Influences: With Consideration of the Elements of Copying, Chance, and Imperfection en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WELLS John Thomas en-aut-sei=WELLS John Thomas en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Humanities and Social Sciences, Okayama University kn-affil=岡山大学大学院社会文化科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=235 end-page=246 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20221214 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=An Electron Tomographic Analysis of Giantin-Deficient Golgi Proposes a New Function of the Golgin Protein Family en-subtitle= kn-subtitle= en-abstract= kn-abstract=The Golgi apparatus is an organelle that mediates modifications, sorting, and transport of proteins and lipids. Golgins are a group of proteins with coiled-coil structures that localize to the Golgi and are thought to function as tethers to facilitate the docking of vesicles, Rab GTPases, and cytoskeleton components to the Golgi stack. Giantin is the longest golgin and has been thought to function as a tether for COPI vesicles along with other golgins, such as p115 and GM130. Contrary to our expectation that the loss of the tether will result in an increase in untethered COPI vesicles in the cytoplasm, our electron microscopy observations showed that the fenestrae normally present in Golgi cisternae were reduced upon Giantin knockdown. We also found that this structural change is accompanied by altered secretion of cargo proteins and cell surface glycosylation. These results indicate that there exists a correlation between Golgi structural changes caused by the loss of Giantin and Golgi function. Here, we describe electron tomography methods for the detection of structural changes in the Golgi. en-copyright= kn-copyright= en-aut-name=SatohAyano en-aut-sei=Satoh en-aut-mei=Ayano kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Hayashi-NishinoMitsuko en-aut-sei=Hayashi-Nishino en-aut-mei=Mitsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishinoKunihiko en-aut-sei=Nishino en-aut-mei=Kunihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University kn-affil= affil-num=2 en-affil=Institute of Scientific and Industrial Research, Osaka University kn-affil= affil-num=3 en-affil=Institute of Scientific and Industrial Research, Osaka University kn-affil= en-keyword=Golgi kn-keyword=Golgi en-keyword=Golgin kn-keyword=Golgin en-keyword=Giantin kn-keyword=Giantin en-keyword=Electron tomography kn-keyword=Electron tomography en-keyword=3D modeling kn-keyword=3D modeling en-keyword=Vesicles kn-keyword=Vesicles END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=5 article-no= start-page=503 end-page=510 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Viral Sequences Are Repurposed for Controlling Antiviral Responses as Non-Retroviral Endogenous Viral Elements en-subtitle= kn-subtitle= en-abstract= kn-abstract=Eukaryotic genomes contain numerous copies of endogenous viral elements (EVEs), most of which are considered endogenous retrovirus (ERV) sequences. Over the past decade, non-retroviral endogenous viral elements (nrEVEs) derived from ancient RNA viruses have been discovered. Several functions have been proposed for these elements, including antiviral defense. This review summarizes the current understanding of nrEVEs derived from RNA viruses, particularly endogenous bornavirus-like elements (EBLs) and endogenous filovirus-like elements (EFLs). EBLs are one of the most extensively studied nrEVEs. The EBL derived from bornavirus nucleoprotein (EBLN) is thought to function as a non-coding RNA or protein that regulates host gene expression or inhibits virus propagation. Ebolavirus and marburgvirus, which are filoviruses, induce severe hemorrhagic fever in humans and nonhuman primates. Although the ecology of filoviruses remains unclear, bats are believed to be potential reservoirs. Based on the knowledge from EBLs, it is postulated that EFLs in the bat genome help to maintain the balance between filovirus infection and the bat’s defense system, which may partially explain why bats act as potential reservoirs. Further research into the functions of nrEVEs could reveal novel antiviral systems and inspire novel antiviral approaches. en-copyright= kn-copyright= en-aut-name=OgawaHirohito en-aut-sei=Ogawa en-aut-mei=Hirohito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HondaTomoyuki en-aut-sei=Honda en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Virology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Department of Virology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=EVE kn-keyword=EVE en-keyword=nrEVE kn-keyword=nrEVE en-keyword=bornavirus kn-keyword=bornavirus en-keyword=filovirus kn-keyword=filovirus en-keyword=antiviral kn-keyword=antiviral END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=4 article-no= start-page=439 end-page=446 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202208 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Social Capital and Post-traumatic Stress Disorder among Heavy Rainfall and Flood Victims in Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=This study examined the relationship between cognitive/structural social capital and post-traumatic stress disorder (PTSD) among victims of heavy rain and flood. Participants were individuals aged≥18 years affected by the July 2018 heavy rainfall in the cities of Kurashiki and Soja, Japan, and living in temporary housing. We distributed five copies of a questionnaire to 1,991 households and received responses from 1,927 individuals (907 men, 1,008 women, 12 respondents of unspecified sex) in 1,029 households (51.7%). We estimated odds ratios (ORs) and 95% confidence intervals (CIs) for associations between high (vs. low) social capital and PTSD or other outcomes. After covariate adjustment, the odds of having PTSD were lower in participants with high cognitive social capital than those with low cognitive social capital (OR=0.346, 95%CI: 0.263-0.456). Elderly women with higher structural social capital tended to have lower PTSD odds than those with lower structural social capital (OR=0.671, 95%CI: 0.431-1.046). The opposite pattern was observed for elderly men (OR=1.315, 95%CI: 0.792-2.183). Cognitive social capital is a protective factor that may reduce PTSD or promote a favorable PTSD prognosis after heavy rainfall and flood events. The associations between structural social capital and PTSD differ by age and sex. en-copyright= kn-copyright= en-aut-name=MiyajiChikara en-aut-sei=Miyaji en-aut-mei=Chikara kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakaoSoshi en-aut-sei=Takao en-aut-mei=Soshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NoguchiMasayuki en-aut-sei=Noguchi en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkazakiTsubasa en-aut-sei=Okazaki en-aut-mei=Tsubasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SatoShunsuke en-aut-sei=Sato en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YorifujiTakashi en-aut-sei=Yorifuji en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Okayama Prefectural Mental Health and Welfare Center kn-affil= affil-num=4 en-affil=Okayama Prefectural Mental Health and Welfare Center kn-affil= affil-num=5 en-affil=Okayama Prefectural Mental Health and Welfare Center kn-affil= affil-num=6 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=social capital kn-keyword=social capital en-keyword=post-traumatic stress disorder kn-keyword=post-traumatic stress disorder en-keyword=disaster kn-keyword=disaster en-keyword=flooding kn-keyword=flooding END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page=59 end-page=73 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=保育施設における幼児の描画活動に見るキャラクター表現と図形模写能力の関連性 en-subtitle= kn-subtitle= en-abstract=This study discusses the relationship between the act of drawing characters and the shapes copying ability of preschoolers observed in drawing activities. For the purpose of elucidating the effects of drawing characters on the children’s shapes copying ability, preschoolers were divided into the following three groups and their shapes copying ability was comparatively examined: 1) those who draw characters, 2) those who do not draw characters but draw in a general way, 3) those who do not participate in drawing activities on their own initiative. As a result, it was elucidated that children who draw characters and children who draw in a general way both had the same level of shapes copying ability. Accordingly, as with the act of drawing in a general way, the act of drawing characters may be regarded as one of the expression forms for building drawing experience. kn-abstract=本論は，幼児の描画活動におけるキャラクター表現と図形模写能力の関連性について考察する。そこで，キャラクター表現の有無が，図形模写能力に与える影響を明らかにするため，①キャラクター表現を行う幼児，②キャラクターは描かないが一般的な描画を行う幼児，③自発的に描画活動を行わない幼児，の３群に分け図形模写能力を比較検討した。その結果，キャラクター表現をする幼児も，一般的な描画をする幼児と同水準の図形模写能力を有していることが，明らかになった。このことから，キャラクター表現は，一般の描画表現と同様に，描画経験の蓄積となる表現形式の１つになり得ると考えられる。 en-copyright= kn-copyright= en-aut-name=Shiota(Seiyo)Yui en-aut-sei=Shiota(Seiyo) en-aut-mei=Yui kn-aut-name=塩田(青陽)結 kn-aut-sei=塩田(青陽) kn-aut-mei=結 aut-affil-num=1 ORCID= en-aut-name=TakahashiKei en-aut-sei=Takahashi en-aut-mei=Kei kn-aut-name=髙橋慧 kn-aut-sei=髙橋 kn-aut-mei=慧 aut-affil-num=2 ORCID= en-aut-name=KatayamaMika en-aut-sei=Katayama en-aut-mei=Mika kn-aut-name=片山美香 kn-aut-sei=片山 kn-aut-mei=美香 aut-affil-num=3 ORCID= affil-num=1 en-affil=Fund-contributed medical corporation Ryutenkai Katagi Neurosurgery Sigma Daycare Center kn-affil=基金拠出型医療法人隆典会片木脳神経外科内シグマ託児所 affil-num=2 en-affil=The Faculty of Ch ildhood Education, Kurashiki Sakuyo University kn-affil=くらしき作陽大学子ども教育学部 affil-num=3 en-affil=Graduate School of Education, Okayama University kn-affil=岡山大学学術研究院教育学域 en-keyword=保育施設(preschool facility) kn-keyword=保育施設(preschool facility) en-keyword=幼児(preschoolers) kn-keyword=幼児(preschoolers) en-keyword=描画活動(drawing activities) kn-keyword=描画活動(drawing activities) en-keyword=キャラクター表現(drawing characters) kn-keyword=キャラクター表現(drawing characters) en-keyword=図形模写能力(shapes copying ability) kn-keyword=図形模写能力(shapes copying ability) END start-ver=1.4 cd-journal=joma no-vol=14 cd-vols= no-issue=1 article-no= start-page=237 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210623 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Development of a method to rapidly assess resistance/susceptibility of Micro-Tom tomatoes to Tomato yellow leaf curl virus via agroinoculation of cotyledons en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objective: Tomato yellow leaf curl virus (TYLCV) is one of the pathogens severely damaging tomato crops. Therefore, methods to treat or prevent TYLCV infection need to be developed. For this purpose, a method to conveniently and quickly assess infection of tomatoes by TYLCV is desired. In the present study, we established a quick method to evaluate TYLCV infection using cotyledons of Micro-Tom, a miniature tomato cultivar.
Results: First, we constructed a binary plasmid harboring 1.5 copies of the TYLCV genome and transformed Agrobacterium with the plasmid. By injecting agroinoculum from the resulting transformant into the branches of Micro-Tom, we confirmed the susceptibility of Micro-Tom to TYLCV. To shorten the evaluation process of TYLCV infection further, we agroinoculated cotyledons of Micro-Tom 10 days after sowing seeds. We consistently observed typical symptoms of TYLCV infection on true leaves 10 days after agroinoculation. Molecular analysis detected TYLCV progeny DNA in all leaves demonstrating symptoms 6 days after agroinoculation. Therefore, our new protocol enabled assessment of TYLCV infection within 20 days after sowing seeds. Thus, agroinoculation of Micro-Tom cotyledons will accelerate the process of screening TYLCV-resistant Micro-Toms and enable screening of larger numbers of plants more quickly, contributing to the development of TYLCV-resistant tomatoes. en-copyright= kn-copyright= en-aut-name=MoriTomoaki en-aut-sei=Mori en-aut-mei=Tomoaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakenakaKosuke en-aut-sei=Takenaka en-aut-mei=Kosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=DomotoFumiya en-aut-sei=Domoto en-aut-mei=Fumiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AoyamaYasuhiro en-aut-sei=Aoyama en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SeraTakashi en-aut-sei=Sera en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Applied Chemistry and Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University kn-affil= affil-num=2 en-affil=Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University kn-affil= affil-num=3 en-affil=Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University kn-affil= affil-num=4 en-affil=Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University kn-affil= affil-num=5 en-affil=Department of Applied Chemistry and Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University kn-affil= en-keyword=Agrobacterium kn-keyword=Agrobacterium en-keyword=Agroinoculation kn-keyword=Agroinoculation en-keyword=Cotyledon kn-keyword=Cotyledon en-keyword=Micro-Tom kn-keyword=Micro-Tom en-keyword=Tomato yellow leaf curl virus kn-keyword=Tomato yellow leaf curl virus END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210121 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Profile of down syndrome–associated malignancies: Epidemiology, clinical features and therapeutic aspects en-subtitle= kn-subtitle= en-abstract= kn-abstract=Down syndrome (DS) is a congenital chromosomal abnormality caused by the presence of all or part of a third copy of chromosome 21 (+21). DS is frequently complicated by congenital heart or digestive tract diseases at birth. DS patients are prone to infections and have mental retardation, with dementia such as Alzheimer's disease showing in later life. Furthermore, malignancies with specific characteristics are also highly reported in DS patients compared with non-DS patients. Therefore, DS is believed to be a cancer predisposition syndrome due to the chromosomal instability. Acute myeloid leukemia (AML) and especially acute megakaryoblastic leukemia (AMKL) by French-American-British (FAB) classification are the most frequent hematological malignancies in DS patients, occurring at a rate that is 500 times higher than that in non-DS patients. Interestingly, transient abnormal myelopoiesis (TAM) is observed in approximately 10% of DS neonates with GATA1 mutations, and most TAM patients are asymptomatic and show spontaneous regression; however, about 10%–20% of TAM cases are fatal because of complications such as fetal effusion, liver fibrosis, and other complications.Acute lymphoblastic leukemia (ALL) is also associated with DS, occurring at a rate that is 20 times higher than that in non-DS patients. Furthermore, the prognosis of DS-ALL patients is poorer than that of non-DS-ALL patients. A recent genetic analysis revealed that more than half of DS-ALL cases have a mutation in the CRLF2–JAK pathway, indicating that JAK inhibitors might have a limited effect for DS-ALL patients.Notably, solid tumors such as neuroblastoma, Wilms tumor, and brain tumor, which are frequently observed in non-DS children, are rarely reported in DS children. The reason remains unknown, but it may be because of the triplication of the Down syndrome critical region 1 (DSCR1) gene on chromosome 21. In adult patients with DS, the expected age-adjusted incidence rates of solid tumors are low compared with age-matched euploid cohorts for most cancers except for testicular cancer. Although the average life expectancy of patients with DS will increase with advances in healthcare, the detailed health problems including cancer rates in older DS patients remain unknown. Therefore, these issues will be needed to be addressed in future studies. en-copyright= kn-copyright= en-aut-name=ShimadaAkira en-aut-sei=Shimada en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Pediatric Hematology, Okayama University Hospital kn-affil= en-keyword=Down syndrome kn-keyword=Down syndrome en-keyword=Acute myeloid leukemia kn-keyword=Acute myeloid leukemia en-keyword=Acute megakaryoblastic leukemia kn-keyword=Acute megakaryoblastic leukemia en-keyword=Transient abnormal myelopoiesis kn-keyword=Transient abnormal myelopoiesis en-keyword=Acute lymphoblastic leukemia kn-keyword=Acute lymphoblastic leukemia en-keyword=Solid tumor kn-keyword=Solid tumor en-keyword=Cancer predisposition syndrome kn-keyword=Cancer predisposition syndrome en-keyword=GATA1 kn-keyword=GATA1 en-keyword=Down syndrome critical region 1 kn-keyword=Down syndrome critical region 1 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=117 end-page=33 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200818 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Insights into the evolution of regulated actin dynamics via characterization of primitive gelsolin/cofilin proteins from Asgard archaea en-subtitle= kn-subtitle= en-abstract= kn-abstract=Asgard archaea genomes contain potential eukaryotic-like genes that provide intriguing insight for the evolution of eukaryotes. The eukaryotic actin polymerization/depolymerization cycle is critical for providing force and structure in many processes, including membrane remodeling. In general, Asgard genomes encode two classes of actin-regulating proteins from sequence analysis, profilins and gelsolins. Asgard profilins were demonstrated to regulate actin filament nucleation. Here, we identify actin filament severing, capping, annealing and bundling, and monomer sequestration activities by gelsolin proteins from Thorarchaeota (Thor), which complete a eukaryotic-like actin depolymerization cycle, and indicate complex actin cytoskeleton regulation in Asgard organisms. Thor gelsolins have homologs in other Asgard archaea and comprise one or two copies of the prototypical gelsolin domain. This appears to be a record of an initial preeukaryotic gene duplication event, since eukaryotic gelsolins are generally comprise three to six domains. X-ray structures of these proteins in complex with mammalian actin revealed similar interactions to the first domain of human gelsolin or cofilin with actin. Asgard two-domain, but not one-domain, gelsolins contain calcium-binding sites, which is manifested in calcium-controlled activities. Expression of two-domain gelsolins in mammalian cells enhanced actin filament disassembly on ionomycin-triggered calcium release. This functional demonstration, at the cellular level, provides evidence for a calcium-controlled Asgard actin cytoskeleton, indicating that the calcium-regulated actin cytoskeleton predates eukaryotes. In eukaryotes, dynamic bundled actin filaments are responsible for shaping filopodia and microvilli. By correlation, we hypothesize that the formation of the protrusions observed from Lokiarchaeota cell bodies may involve the gelsolin-regulated actin structures. en-copyright= kn-copyright= en-aut-name=AkılCaner en-aut-sei=Akıl en-aut-mei=Caner kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TranLinh T. en-aut-sei=Tran en-aut-mei=Linh T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Orhant-PriouxMagali en-aut-sei=Orhant-Prioux en-aut-mei=Magali kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=BaskaranYohendran en-aut-sei=Baskaran en-aut-mei=Yohendran kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ManserEdward en-aut-sei=Manser en-aut-mei=Edward kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=BlanchoinLaurent en-aut-sei=Blanchoin en-aut-mei=Laurent kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=RobinsonRobert C. en-aut-sei=Robinson en-aut-mei=Robert C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Institute of Molecular and Cell Biology, Agency for Science, Technology and Research kn-affil= affil-num=2 en-affil=Research Institute for Interdisciplinary Science, Okayama University kn-affil= affil-num=3 en-affil=CytomorphoLab, Interdisciplinary Research Institute of Grenoble kn-affil= affil-num=4 en-affil=aInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research kn-affil= affil-num=5 en-affil=aInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research kn-affil= affil-num=6 en-affil=CytomorphoLab, Interdisciplinary Research Institute of Grenoble kn-affil= affil-num=7 en-affil=cResearch Institute for Interdisciplinary Science, Okayama University kn-affil= en-keyword=actin kn-keyword=actin en-keyword=gelsolin kn-keyword=gelsolin en-keyword=Asgard archaea kn-keyword=Asgard archaea en-keyword=eukaryogenesis kn-keyword=eukaryogenesis en-keyword=X-ray crystallography kn-keyword=X-ray crystallography END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=4798 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Development of an experimental method of systematically estimating protein expression limits in HEK293 cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=Protein overexpression sometimes causes cellular defects, although the underlying mechanism is still unknown. A protein's expression limit, which triggers cellular defects, is a useful indication of the underlying mechanism. In this study, we developed an experimental method of estimating the expression limits of target proteins in the human embryonic kidney cell line HEK293 by measuring the proteins' expression levels in cells that survived after the high-copy introduction of plasmid DNA by which the proteins were expressed under a strong cytomegalovirus promoter. The expression limits of nonfluorescent target proteins were indirectly estimated by measuring the levels of green fluorescent protein (GFP) connected to the target proteins with the self-cleaving sequence P2A. The expression limit of a model GFP was similar to 5.0% of the total protein, and sustained GFP overexpression caused cell death. The expression limits of GFPs with mitochondria-targeting signals and endoplasmic reticulum localization signals were 1.6% and 0.38%, respectively. The expression limits of four proteins involved in vesicular trafficking were far lower compared to a red fluorescent protein. The protein expression limit estimation method developed will be valuable for defining toxic proteins and consequences of protein overexpression. en-copyright= kn-copyright= en-aut-name=MoriYoshihiro en-aut-sei=Mori en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshidaYuki en-aut-sei=Yoshida en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatohAyano en-aut-sei=Satoh en-aut-mei=Ayano kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriyaHisao en-aut-sei=Moriya en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Sony Computer Science Laboratories kn-affil= affil-num=3 en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University kn-affil= affil-num=4 en-affil=Research Core for Interdisciplinary Sciences, Okayama University kn-affil= en-keyword=Biological techniques kn-keyword=Biological techniques en-keyword=Cell biology kn-keyword=Cell biology en-keyword=Gene expression analysis kn-keyword=Gene expression analysis en-keyword=Molecular biology kn-keyword=Molecular biology en-keyword=Protein translocation kn-keyword=Protein translocation en-keyword=Protein transport kn-keyword=Protein transport END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=12581 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200728 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing en-subtitle= kn-subtitle= en-abstract= kn-abstract=The use of primary patient-derived organoids for drug sensitivity and resistance testing could play an important role in precision cancer medicine. We developed expandable ovarian cancer organoids in<3 weeks; these organoids captured the characteristics of histological cancer subtypes and replicated the mutational landscape of the primary tumours. Seven pairs of organoids (3 high-grade serous, 1 clear cell, 3 endometrioid) and original tumours shared 59.5% (36.1-73.1%) of the variants identified. Copy number variations were also similar among organoids and primary tumours. The organoid that harboured the BRCA1 pathogenic variant (p.L63*) showed a higher sensitivity to PARP inhibitor, olaparib, as well as to platinum drugs compared to the other organoids, whereas an organoid derived from clear cell ovarian cancer was resistant to conventional drugs for ovarian cancer, namely platinum drugs, paclitaxel, and olaparib. The overall success rate of primary organoid culture, including those of various histological subtypes, was 80% (28/35). Our data show that patient-derived organoids are suitable physiological ex vivo cancer models that can be used to screen effective personalised ovarian cancer drugs. en-copyright= kn-copyright= en-aut-name=NankiYoshiko en-aut-sei=Nanki en-aut-mei=Yoshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ChiyodaTatsuyuki en-aut-sei=Chiyoda en-aut-mei=Tatsuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HirasawaAkira en-aut-sei=Hirasawa en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OokuboAki en-aut-sei=Ookubo en-aut-mei=Aki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ItohManabu en-aut-sei=Itoh en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=UenoMasaru en-aut-sei=Ueno en-aut-mei=Masaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AkahaneTomoko en-aut-sei=Akahane en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KameyamaKaori en-aut-sei=Kameyama en-aut-mei=Kaori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamagamiWataru en-aut-sei=Yamagami en-aut-mei=Wataru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KataokaFumio en-aut-sei=Kataoka en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=AokiDaisuke en-aut-sei=Aoki en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Department of Obstetrics and Gynecology, Keio University School of Medicine kn-affil= affil-num=2 en-affil=Department of Obstetrics and Gynecology, Keio University School of Medicine kn-affil= affil-num=3 en-affil=Department of Clinical Genomic Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=JSR‑Keio University Medical and Chemical Innovation Center (JKiC), JSR Corp. kn-affil= affil-num=5 en-affil=JSR‑Keio University Medical and Chemical Innovation Center (JKiC), JSR Corp. kn-affil= affil-num=6 en-affil=JSR‑Keio University Medical and Chemical Innovation Center (JKiC), JSR Corp. kn-affil= affil-num=7 en-affil=JSR‑Keio University Medical and Chemical Innovation Center (JKiC), Keio University School of Medicine kn-affil= affil-num=8 en-affil=Department of Pathology, Keio University School of Medicine kn-affil= affil-num=9 en-affil=Department of Obstetrics and Gynecology, Keio University School of Medicine kn-affil= affil-num=10 en-affil=Department of Obstetrics and Gynecology, Keio University School of Medicine kn-affil= affil-num=11 en-affil=Department of Obstetrics and Gynecology, Keio University School of Medicine kn-affil= END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=2 article-no= start-page=121 end-page=132 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200514 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The expression level and cytotoxicity of green fluorescent protein are modulated by an additional N-terminal sequence en-subtitle= kn-subtitle= en-abstract= kn-abstract=Nucleotide and amino acid sequences at the N-terminus affect the expression level and cytotoxicity of proteins; however, their effects are not fully understood yet. Here, N-terminal 30 nucleotide/10 amino acid (N10) sequences that affect the expression level and cytotoxicity of a green fluorescent protein were systematically isolated in the budding yeast Saccharomyces cerevisiae. The expression per gene (EPG) and gene copy number limit (CNL) relationships were examined to assess the effects of the N10 sequence. The isolated N10 nucleotide sequences suggested that codon optimality is the major determinant of the protein expression level. A higher number of hydrophobic or cysteine residues in the N10 sequence seemed to increase the cytotoxicity of the protein. Therefore, a high frequency of specific amino acid residues in the outside of the main tertiary structure of proteins might not be preferable. en-copyright= kn-copyright= en-aut-name=MoriyaHisao en-aut-sei=Moriya en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Sciences, Research Core for Interdisciplinary Sciences, Okayama University kn-affil= en-keyword=green fluorescent protein kn-keyword=green fluorescent protein en-keyword=overexpression kn-keyword=overexpression en-keyword=expression limit kn-keyword=expression limit en-keyword=expression level kn-keyword=expression level en-keyword=protein cytotoxicity kn-keyword=protein cytotoxicity END start-ver=1.4 cd-journal=joma no-vol=40 cd-vols= no-issue=6 article-no= start-page=3981 end-page=3995 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Axisymmetric traveling fronts in balanced bistable reaction-diffusion equations en-subtitle= kn-subtitle= en-abstract= kn-abstract=For a balanced bistable reaction-diffusion equation, the existence of axisymmetric traveling fronts has been studied by Chen, Guo, Ninomiya, Hamel and Roquejoffre [4]. This paper gives another proof of the existence of axisymmetric traveling fronts. Our method is as follows. We use pyramidal traveling fronts for unbalanced reaction-diffusion equations, and take the balanced limit. Then we obtain axisymmetric traveling fronts in a balanced bistable reaction-diffusion equation. Since pyramidal traveling fronts have been studied in many equations or systems, our method might be applicable to study axisymmetric traveling fronts in these equations or systems. en-copyright= kn-copyright= en-aut-name=TaniguchiMasaharu en-aut-sei=Taniguchi en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= Research Institute for Interdisciplinary Science, Okayama University kn-affil= en-keyword= Traveling front kn-keyword= Traveling front en-keyword= reaction-diffusion equation kn-keyword= reaction-diffusion equation en-keyword=axisymmetric kn-keyword=axisymmetric en-keyword=balanced kn-keyword=balanced END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue=5 article-no= start-page=1564 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Retrotransposons Manipulating Mammalian Skeletal Development in Chondrocytes en-subtitle= kn-subtitle= en-abstract= kn-abstract=Retrotransposons are genetic elements that copy and paste themselves in the host genome through transcription, reverse-transcription, and integration processes. Along with their proliferation in the genome, retrotransposons inevitably modify host genes around the integration sites, and occasionally create novel genes. Even now, a number of retrotransposons are still actively editing our genomes. As such, their profound role in the evolution of mammalian genomes is obvious; thus, their contribution to mammalian skeletal evolution and development is also unquestionable. In mammals, most of the skeletal parts are formed and grown through a process entitled endochondral ossification, in which chondrocytes play central roles. In this review, current knowledge on the evolutional, physiological, and pathological roles of retrotransposons in mammalian chondrocyte differentiation and cartilage development is summarized. The possible biological impact of these mobile genetic elements in the future is also discussed. en-copyright= kn-copyright= en-aut-name=KubotaSatoshi en-aut-sei=Kubota en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshikawaTakanori en-aut-sei=Ishikawa en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawataKazumi en-aut-sei=Kawata en-aut-mei=Kazumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HattoriTakako en-aut-sei=Hattori en-aut-mei=Takako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishidaTakashi en-aut-sei=Nishida en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=retrotransposon kn-keyword=retrotransposon en-keyword=endogenous retrovirus kn-keyword=endogenous retrovirus en-keyword=chondrocyte kn-keyword=chondrocyte en-keyword=cartilage kn-keyword=cartilage en-keyword=skeletal development kn-keyword=skeletal development END start-ver=1.4 cd-journal=joma no-vol=16 cd-vols= no-issue=2 article-no= start-page=e1008566 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200218 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The persimmon genome reveals clues to the evolution of a lineage-specific sex determination system in plants en-subtitle= kn-subtitle= en-abstract= kn-abstract=Most angiosperms bear hermaphroditic flowers, but a few species have evolved outcrossing strategies, such as dioecy, the presence of separate male and female individuals. We previously investigated the mechanisms underlying dioecy in diploid persimmon (D. lotus) and found that male flowers are specified by repression of the autosomal gene MeGI by its paralog, the Y-encoded pseudo-gene OGI. This mechanism is thought to be lineage-specific, but its evolutionary path remains unknown. Here, we developed a full draft of the diploid persimmon genome (D. lotus), which revealed a lineage-specific whole-genome duplication event and provided information on the architecture of the Y chromosome. We also identified three paralogs, MeGI, OGI and newly identified Sister of MeGI (SiMeGI). Evolutionary analysis suggested that MeGI underwent adaptive evolution after the whole-genome duplication event. Transformation of tobacco plants with MeGI and SiMeGI revealed that MeGI specifically acquired a new function as a repressor of male organ development, while SiMeGI presumably maintained the original function. Later, a segmental duplication event spawned MeGI's regulator OGI on the Y-chromosome, completing the path leading to dioecy, and probably initiating the formation of the Y-chromosome. These findings exemplify how duplication events can provide flexible genetic material available to help respond to varying environments and provide interesting parallels for our understanding of the mechanisms underlying the transition into dieocy in plants. Author summary Plant sexuality has fascinated scientists for decades. Most plants can self-reproduce but not all. For example, a small subset of species have evolved a system called dioecy, with separate male and female individuals. Dioecy has evolved multiple times independently and, while we do not understand the molecular mechanisms underlying dioecy in many of these species yet, a picture is starting to emerge with recent progress in several dioecious species. Here, we focused on the evolutionary events leading to dioecy in persimmon. Our previous work had identified a pair of genes regulating sex in this species, called OGI and MeGI. We drafted the whole genome sequence of diploid persimmon to investigate their evolutionary history. We discovered a lineage-specific whole-genome duplication event, and observed that MeGI underwent adaptive evolution after this event. Transgenic analyses validated that MeGI newly acquired a male-suppressor function, while the other copy of this gene, SiMeGI, did not. The regulator of MeGI, OGI, resulted from a second smaller-scale segmental duplication event, finalizing the system. This study sheds light on the role of duplication as a mechanism that promote flexible genes functions, and how it can affect important biological functions, such as the establishment of a new sexual system. en-copyright= kn-copyright= en-aut-name=AkagiTakashi en-aut-sei=Akagi en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShirasawaKenta en-aut-sei=Shirasawa en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagasakiHideki en-aut-sei=Nagasaki en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HirakawaHideki en-aut-sei=Hirakawa en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaoRyutaro en-aut-sei=Tao en-aut-mei=Ryutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ComaiLuca en-aut-sei=Comai en-aut-mei=Luca kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HenryIsabelle M. en-aut-sei=Henry en-aut-mei=Isabelle M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Kazusa DNA Research Institute kn-affil= affil-num=3 en-affil=Kazusa DNA Research Institute kn-affil= affil-num=4 en-affil=Kazusa DNA Research Institute kn-affil= affil-num=5 en-affil=Graduate School of Agriculture, Kyoto University kn-affil= affil-num=6 en-affil=Genome Center and Department of Plant Biology, University of California Davis kn-affil= affil-num=7 en-affil=Genome Center and Department of Plant Biology, University of California Davis kn-affil= END start-ver=1.4 cd-journal=joma no-vol=109 cd-vols= no-issue= article-no= start-page=7 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells kn-title=酵母2μプラスミドのコピー数変動の数理的解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract= 　Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency （fitness） produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy. en-copyright= kn-copyright= en-aut-name=SaekiNozomu en-aut-sei=Saeki en-aut-mei=Nozomu kn-aut-name=佐伯望 kn-aut-sei=佐伯 kn-aut-mei=望 aut-affil-num=1 ORCID= en-aut-name=MoriyaHisao en-aut-sei=Moriya en-aut-mei=Hisao kn-aut-name=守屋央朗 kn-aut-sei=守屋 kn-aut-mei=央朗 aut-affil-num=2 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University) kn-affil=岡山大学大学院　環境生命科学研究科 affil-num=2 en-affil= Research Core for Interdisciplinary Sciences, Okayama University kn-affil=異分野融合先端研究コア en-keyword=yeast kn-keyword=yeast en-keyword=2 μ plasmid kn-keyword=2 μ plasmid en-keyword=mathematical model kn-keyword=mathematical model END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue= article-no= start-page=8866 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=2019620 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Benzyl isothiocyanate (BITC) is a naturally-occurring isothiocyanate derived from cruciferous vegetables. BITC has been reported to inhibit the proliferation of various cancer cells, which is believed to be important for the inhibition of tumorigenesis. However, the detailed mechanisms of action remain unclear. In this study, we employed a budding yeast Saccharomyces cerevisiae as a model organism for screening. Twelve genes including MTW1 were identified as the overexpression suppressors for the antiproliferative effect of BITC using the genome-wide multi-copy plasmid collection for S. cerevisiae. Overexpression of the kinetochore protein Mtw1 counteracts the antiproliferative effect of BITC in yeast. The inhibitory effect of BITC on the proliferation of human colon cancer HCT-116 cells was consistently suppressed by the overexpression of Mis12, a human orthologue of Mtw1, and enhanced by the knockdown of Mis12. We also found that BITC increased the phosphorylated and ubiquitinated Mis12 level with consequent reduction of Mis12, suggesting that BITC degrades Mis12 through an ubiquitin-proteasome system. Furthermore, cell cycle analysis showed that the change in the Mis12 level affected the cell cycle distribution and the sensitivity to the BITC-induced apoptosis. These results provide evidence that BITC suppresses cell proliferation through the post-transcriptional regulation of the kinetochore protein Mis12. en-copyright= kn-copyright= en-aut-name=Abe-KanohNaomi en-aut-sei=Abe-Kanoh en-aut-mei=Naomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KunisueNarumi en-aut-sei=Kunisue en-aut-mei=Narumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MyojinTakumi en-aut-sei=Myojin en-aut-mei=Takumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ChinoAyako en-aut-sei=Chino en-aut-mei=Ayako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MunemasaShintaro en-aut-sei=Munemasa en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurataYoshiyuki en-aut-sei=Murata en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SatohAyano en-aut-sei=Satoh en-aut-mei=Ayano kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MoriyaHisao en-aut-sei=Moriya en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NakamuraYoshimasa en-aut-sei=Nakamura en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Research Core for Interdisciplinary Sciences, Okayama University kn-affil= affil-num=5 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=6 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=7 en-affil= Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=8 en-affil=Research Core for Interdisciplinary Sciences, Okayama University kn-affil= affil-num=9 en-affil=Graduate School of Environmental and Life Science, Okayama University, Okayama kn-affil= END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=11 article-no= start-page=1001 end-page=1007 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20191005 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Yoga-plus exercise mix promotes cognitive, affective, and physical functions in elderly people en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objectives:
Increased attention is being paid to Asian medicine in balanced total health care. We investigated the effects of mixed exercise including yoga ('Yoga-plus') among elderly individuals.
Methods:
A total of 385 subjects (72 males and 313 females, 75.5 ± 8.7 years old) participated in a 12-month (M) exercise program at a health and welfare center, a day service center, and a nursing home. Cognitive, affective, and physical functions, and activities of daily living (ADL), were compared at baseline (0M), 6M and 12M of exercise intervention.
Results:
Mean scores on the frontal assessment battery, clock drawing test, cube copying test, letter fluency, and category fluency significantly improved after the Yoga-plus intervention, while mini-mental state examination, Hasegawa dementia score-revised, and trail-making test performance were relatively stable. Affective scores on the geriatric depression scale (GDS), apathy scale (AS) and Abe's behavioral and psychological symptoms of dementia were not significantly affected by exercise therapy, but subgroups with higher baseline GDS (GDS ≥ 5) and AS (AS ≥ 16) scores showed a significant improvement after intervention. One-leg standing time and 3-m timed up and go test performance significantly improved after 12M intervention.
Discussion:
Yoga-plus improved cognitive, affective, ADL, and physical functions in a local elderly population, particularly among below-baseline individuals, indicating the benefits of dementia prevention among elderly individuals. en-copyright= kn-copyright= en-aut-name=HishikawaNozomi en-aut-sei=Hishikawa en-aut-mei=Nozomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakahashiYoriko en-aut-sei=Takahashi en-aut-mei=Yoriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FukuiYusuke en-aut-sei=Fukui en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TokuchiRyo en-aut-sei=Tokuchi en-aut-mei=Ryo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FurusawaJunichi en-aut-sei=Furusawa en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakemotoMami en-aut-sei=Takemoto en-aut-mei=Mami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SatoKota en-aut-sei=Sato en-aut-mei=Kota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=YamashitaToru en-aut-sei=Yamashita en-aut-mei=Toru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OhtaYasuyuki en-aut-sei=Ohta en-aut-mei=Yasuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=AbeKoji en-aut-sei=Abe en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Education, Nippon Ayurveda School kn-affil= affil-num=3 en-affil= Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Occupational Therapy, Okayama Institute for Medical and Technical Sciences kn-affil= affil-num=5 en-affil=Department of Occupational Therapy, Mizunaga Rehabilitation Hospital kn-affil= affil-num=6 en-affil= Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil= Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil= Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil= Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil= Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Affective function kn-keyword=Affective function en-keyword=cognitive function kn-keyword=cognitive function en-keyword=elderly population kn-keyword=elderly population en-keyword=physical function kn-keyword=physical function en-keyword=yoga exercise kn-keyword=yoga exercise END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue= article-no= start-page=46 end-page=58 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Contents of the Proceedings of the Okayama Association for Laboratory Animal Science : From No.1 to 35 kn-title=岡山実験動物研究会報第1 号から第３５号までの掲載内容 en-subtitle= kn-subtitle= en-abstract= kn-abstract= The Proceedings of the Okayama Association for Laboratory Animal Science were initially issued in 1983, the year following the establishment of the association, and No.35 is the latest.Scince issue No.2, the proceedings have consisted of the following content: , , , , , and , in this order. Additionally, the publication of references and advertisements by supporting members recently started. Number 19 (issued in 2002) was the first to present a table of contents in English on its back cover, and this style has been maintained to date. On March 24, the president of the association and director of the Okayama University Libraries exchanged copies of an agreement regarding the registration of these contents with the Okayama University Scientific Achievement Repository (OUSAR) and other databases, providing a basis for their current registration and publication. Literature-based information is also being collected and published in databases organized by the Non-profit Organization Japan Medical Abstracts Society (Ichushi) and Japan Science & Technology Agency (JST). en-copyright= kn-copyright= en-aut-name=SatoKatsunori en-aut-sei=Sato en-aut-mei=Katsunori kn-aut-name=佐藤勝紀 kn-aut-sei=佐藤 kn-aut-mei=勝紀 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Animal Resources, Tsushima South Laboratory, Advanced Science Research Center, Okayama University kn-affil=岡山大学自然生命科学研究支援センター・動物資源部門・津島南施設 END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue= article-no= start-page=1250 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160809 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Phenotypic and Genetic Heterogeneity in Vibrio cholerae O139 Isolated from Cholera Cases in Delhi, India during 2001-2006 en-subtitle= kn-subtitle= en-abstract= kn-abstract= Incidence of epidemic Vibrio cholerae serogroup O139 has declined in cholera endemic countries. However, sporadic cholera caused by V. cholerae O139 with notable genetic changes is still reported from many regions. In the present study, 42 V. cholerae O139 strains isolated from 2001 to 2006 in Delhi, India, were retrospectively analyzed to understand their phenotype and molecular characteristics. The majority of isolates were resistant to ampicillin, furazolidone and nalidixic acid. Though the integrative conjugative element was detected in all the O139 isolates, the 2004–2006 isolates remained susceptible to co-trimoxazole, chloramphenicol, and streptomycin. Cholera toxin genotype 1 was present in the majority of the O139 isolates while few had type 3 or a novel type 4. In the cholera toxin encoding gene (ctx) restriction fragment length polymorphism, the majority of the isolates harbored three copies of CTX element, of which one was truncated. In this study, the ctx was detected for the first time in the small chromosome of V. cholerae O139 and one isolate harbored 5 copies of CTX element, of which 3 were truncated. The ribotype BII pattern was found in most of the O139 isolates. Three V. cholerae O139 isolated in 2001 had a new ribotype BVIII. Pulsed-field gel electrophoresis analysis revealed clonal variation in 2001 isolates compared to the 2004–2006 isolates. Molecular changes in V. cholerae O139 have to be closely monitored as this information may help in understanding the changing genetic features of this pathogen in relation to the epidemiology of cholera. en-copyright= kn-copyright= en-aut-name=GhoshRaikamal en-aut-sei=Ghosh en-aut-mei=Raikamal kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SharmaNaresh C. en-aut-sei=Sharma en-aut-mei=Naresh C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HalderKalpataru en-aut-sei=Halder en-aut-mei=Kalpataru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=BhadraRupak K. en-aut-sei=Bhadra en-aut-mei=Rupak K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ChowdhuryGoutam en-aut-sei=Chowdhury en-aut-mei=Goutam kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=PazhaniGururaja P. en-aut-sei=Pazhani en-aut-mei=Gururaja P. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShinodaSumio en-aut-sei=Shinoda en-aut-mei=Sumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MukhopadhyayAsish K. en-aut-sei=Mukhopadhyay en-aut-mei=Asish K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NairG. Balakrish en-aut-sei=Nair en-aut-mei=G. Balakrish kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=RamamurthyThadavarayan en-aut-sei=Ramamurthy en-aut-mei=Thadavarayan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases kn-affil= affil-num=2 en-affil=Maharishi Valmiki Infectious Diseases Hospital kn-affil= affil-num=3 en-affil=Infectious Diseases and Immunology Division, Council of Scientific and Industrial Research-Indian Institute of Chemical Biology kn-affil= affil-num=4 en-affil=Infectious Diseases and Immunology Division, Council of Scientific and Industrial Research-Indian Institute of Chemical Biology kn-affil= affil-num=5 en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases kn-affil= affil-num=6 en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases kn-affil= affil-num=7 en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases kn-affil= affil-num=8 en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases kn-affil= affil-num=9 en-affil=Center for Human Microbial Ecology, Translational Health Science and Technology Institute kn-affil= affil-num=10 en-affil=Center for Human Microbial Ecology, Translational Health Science and Technology Institute kn-affil= en-keyword=V.cholerae O139 kn-keyword=V.cholerae O139 en-keyword=ribotypes kn-keyword=ribotypes en-keyword=CT genotype kn-keyword=CT genotype en-keyword=CTX prophage kn-keyword=CTX prophage en-keyword=PFGE kn-keyword=PFGE END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue= article-no= start-page=1044 end-page=1055 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Organelle DNA degradation contributes to the efficient use of phosphate in seed plants en-subtitle= kn-subtitle= en-abstract= kn-abstract= Mitochondria and chloroplasts (plastids) both harbour extranuclear DNA that originates from the ancestral endosymbiotic bacteria. These organelle DNAs (orgDNAs) encode limited genetic information but are highly abundant, with multiple copies in vegetative tissues, such as mature leaves. Abundant orgDNA constitutes a substantial pool of organic phosphate along with RNA in chloroplasts, which could potentially contribute to phosphate recycling when it is degraded and relocated. However, whether orgDNA is degraded nucleolytically in leaves remains unclear. In this study, we revealed the prevailing mechanism in which organelle exonuclease DPD1 degrades abundant orgDNA during leaf senescence. The DPD1 degradation system is conserved in seed plants and, more remarkably, we found that it was correlated with the efficient use of phosphate when plants were exposed to nutrient-deficient conditions. The loss of DPD1 compromised both the relocation of phosphorus to upper tissues and the response to phosphate starvation, resulting in reduced plant fitness. Our findings highlighted that DNA is also an internal phosphate-rich reservoir retained in organelles since their endosymbiotic origin. en-copyright= kn-copyright= en-aut-name=TakamiTsuneaki en-aut-sei=Takami en-aut-mei=Tsuneaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhnishiNorikazu en-aut-sei=Ohnishi en-aut-mei=Norikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KuritaYuko en-aut-sei=Kurita en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IwamuraShoko en-aut-sei=Iwamura en-aut-mei=Shoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhnishiMiwa en-aut-sei=Ohnishi en-aut-mei=Miwa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KusabaMakoto en-aut-sei=Kusaba en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MimuraTetsuro en-aut-sei=Mimura en-aut-mei=Tetsuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SakamotoWataru en-aut-sei=Sakamoto en-aut-mei=Wataru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil= Faculty of Agriculture, Ryukoku University kn-affil= affil-num=4 en-affil=Department of Biology, Graduate School of Science, Kobe University kn-affil= affil-num=5 en-affil=Department of Biology, Graduate School of Science, Kobe University kn-affil= affil-num=6 en-affil=Graduate School of Science, Hiroshima University kn-affil= affil-num=7 en-affil=Department of Biology, Graduate School of Science, Kobe University kn-affil= affil-num=8 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=18577 end-page=18577 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=201512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The molecular mechanism of photochemical internalization of cell penetrating peptide-cargo-photosensitizer conjugates. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In many drug delivery strategies, an inefficient transfer of macromolecules such as proteins and nucleic acids to the cytosol often occurs because of their endosomal entrapment. One of the methods to overcome this problem is photochemical internalization, which is achieved using a photosensitizer and light to facilitate the endosomal escape of the macromolecule. In this study, we examined the molecular mechanism of photochemical internalization of cell penetrating peptide-cargo (macromolecule)-photosensitizer conjugates. We measured the photophysical properties of eight dyes (photosensitizer candidates) and determined the respective endosomal escape efficiencies using these dyes. Correlation plots between these factors indicated that the photogenerated (1)O2 molecules from photosensitizers were highly related to the endosomal escape efficiencies. The contribution of (1)O2 was confirmed using (1)O2 quenchers. In addition, time-lapse fluorescence imaging showed that the photoinduced endosomal escape occurred at a few seconds to a few minutes after irradiation (much longer than (1)O2 lifetime), and that the pH increased in the endosome prior to the endosomal escape of the macromolecule. en-copyright= kn-copyright= en-aut-name=OhtsukiTakashi en-aut-sei=Ohtsuki en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MikiShunya en-aut-sei=Miki en-aut-mei=Shunya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiShouhei en-aut-sei=Kobayashi en-aut-mei=Shouhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HaraguchiTokuko en-aut-sei=Haraguchi en-aut-mei=Tokuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakataEiji en-aut-sei=Nakata en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HirakawaKazutaka en-aut-sei=Hirakawa en-aut-mei=Kazutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SumitaKensuke en-aut-sei=Sumita en-aut-mei=Kensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=WatanabeKazunori en-aut-sei=Watanabe en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OkazakiShigetoshi en-aut-sei=Okazaki en-aut-mei=Shigetoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Medical Bioengineering, Okayama University kn-affil= affil-num=2 en-affil=Department of Medical Bioengineering, Okayama University kn-affil= affil-num=3 en-affil=Advanced ICT Research Institute Kobe, NICT kn-affil= affil-num=4 en-affil=Advanced ICT Research Institute Kobe, NICT kn-affil= affil-num=5 en-affil=Institute of Advanced Energy, Kyoto University kn-affil= affil-num=6 en-affil=Department of Applied Chemistry and Biochemical Engineering, Graduate School of Engineering, Shizuoka University kn-affil= affil-num=7 en-affil=Department of Medical Bioengineering, Okayama University kn-affil= affil-num=8 en-affil=Department of Medical Bioengineering, Okayama University kn-affil=岡山大学大学院自然科学研究科 affil-num=9 en-affil=Department of Medical Spectroscopy, Hamamatsu University School of Medicine kn-affil= END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue= article-no= start-page=12501 end-page=12501 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=201608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Phototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs en-subtitle= kn-subtitle= en-abstract= kn-abstract=The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells. en-copyright= kn-copyright= en-aut-name=OhtsukiTakashi en-aut-sei=Ohtsuki en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KanzakiShigeto en-aut-sei=Kanzaki en-aut-mei=Shigeto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishimuraSae en-aut-sei=Nishimura en-aut-mei=Sae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KunihiroYoshio en-aut-sei=Kunihiro en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SisidoMasahiko en-aut-sei=Sisido en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WatanabeKazunori en-aut-sei=Watanabe en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Biomedical Engineering, Okayama University kn-affil= affil-num=2 en-affil=Department of Biomedical Engineering, Okayama University kn-affil= affil-num=3 en-affil=Department of Biomedical Engineering, Okayama University kn-affil= affil-num=4 en-affil=Department of Biomedical Engineering, Okayama University kn-affil= affil-num=5 en-affil=Department of Biomedical Engineering, Okayama University kn-affil= affil-num=6 en-affil=Department of Biomedical Engineering, Okayama University kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue= article-no= start-page=21487 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160216 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Chemical control of xylem differentiation by thermospermine, xylemin, and auxin en-subtitle= kn-subtitle= en-abstract= kn-abstract=The xylem conducts water and minerals from the root to the shoot and provides mechanical strength to the plant body. The vascular precursor cells of the procambium differentiate to form continuous vascular strands, from which xylem and phloem cells are generated in the proper spatiotemporal pattern. Procambium formation and xylem differentiation are directed by auxin. In angiosperms, thermospermine, a structural isomer of spermine, suppresses xylem differentiation by limiting auxin signalling. However, the process of auxin-inducible xylem differentiation has not been fully elucidated and remains difficult to manipulate. Here, we found that an antagonist of spermidine can act as an inhibitor of thermospermine biosynthesis and results in excessive xylem differentiation, which is a phenocopy of a thermospermine-deficient mutant acaulis5 in Arabidopsis thaliana. We named this compound xylemin owing to its xylem-inducing effect. Application of a combination of xylemin and thermospermine to wild-type seedlings negates the effect of xylemin, whereas co-treatment with xylemin and a synthetic proauxin, which undergoes hydrolysis to release active auxin, has a synergistic inductive effect on xylem differentiation. Thus, xylemin may serve as a useful transformative chemical tool not only for the study of thermospermine function in various plant species but also for the control of xylem induction and woody biomass production. en-copyright= kn-copyright= en-aut-name=YoshimotoKaori en-aut-sei=Yoshimoto en-aut-mei=Kaori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakamuraHiroyoshi en-aut-sei=Takamura en-aut-mei=Hiroyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KadotaIsao en-aut-sei=Kadota en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MotoseHiroyasu en-aut-sei=Motose en-aut-mei=Hiroyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakahashiTaku en-aut-sei=Takahashi en-aut-mei=Taku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil=Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=5 en-affil= kn-affil=Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=1 article-no= start-page=1 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=201602 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Entecavir Reduces Hepatocarcinogenesis in Chronic Hepatitis B Patients en-subtitle= kn-subtitle= en-abstract= kn-abstract=Chronic hepatitis B (CHB) leads to cirrhosis and hepatocellular carcinoma (HCC). With a cohort of 1,206 CHB patients who visited Okayama University Hospital and related hospitals in 2011 and 2012, we compared the incidence rates of HCC among the patients grouped by age, hepatitis B virus (HBV) DNA, hepatitis B e antigen (HBeAg), and treatment. HCCs were observed in 115 patients with the median observation period of 1,687 days. Among the HCC patients aged ≥ 35 years, HBV DNA ≥ 4 log copies/mL and positive HBeAg at diagnosis (n=184), the HCC incidence rate was 8.4% at 5 years in the entecavir (ETV)-treated patients, 21.8% in the lamivudine (LVD)-treated patients, and 26.4% among the patients not treated with drugs. The cumulative HCC incidence was significantly reduced in the ETV-treated patients compared to those treated with LVD or not treated (p=0.013). Among the patients aged ≥ 35 years with HBV DNA ≥ 4 log copies/mL and negative HBeAg (n=237), the cumulative HCC incidence was 14.6% in 5 years in ETV group and 13.9% among those not treated with a drug (p>0.05). Only small numbers of HCCs occurred in other patients. In CHB patients aged≥35 years with HBV DNA ≥4 log copies/mL and positive HBeAg, ETV treatment is recommended for the suppression of HCC development. en-copyright= kn-copyright= en-aut-name=YasunakaTetsuya en-aut-sei=Yasunaka en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IkedaFusao en-aut-sei=Ikeda en-aut-mei=Fusao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WadaNozomu en-aut-sei=Wada en-aut-mei=Nozomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MorimotoYuki en-aut-sei=Morimoto en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FujiokaShin-ichi en-aut-sei=Fujioka en-aut-mei=Shin-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ToshimoriJunichi en-aut-sei=Toshimori en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KobashiHaruhiko en-aut-sei=Kobashi en-aut-mei=Haruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KariyamaKazuya en-aut-sei=Kariyama en-aut-mei=Kazuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MorimotoYoichi en-aut-sei=Morimoto en-aut-mei=Yoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TakayamaHiroki en-aut-sei=Takayama en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=SenoTomonori en-aut-sei=Seno en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TakaguchiKoichi en-aut-sei=Takaguchi en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=MoriyaAkio en-aut-sei=Moriya en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=MiyatakeHirokazu en-aut-sei=Miyatake en-aut-mei=Hirokazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=OkamotoRyoichi en-aut-sei=Okamoto en-aut-mei=Ryoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=YabushitaKazuhisa en-aut-sei=Yabushita en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=TakakiAkinobu en-aut-sei=Takaki en-aut-mei=Akinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=YamamotoKazuhide en-aut-sei=Yamamoto en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= affil-num=1 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Internal Medicine, Okayama Saiseikai General hospital affil-num=6 en-affil= kn-affil=Department of Gastroenterology, Okayama Red Cross Hospital affil-num=7 en-affil= kn-affil=Department of Gastroenterology, Okayama Red Cross Hospital affil-num=8 en-affil= kn-affil=Department of Liver Disease Center, Okayama City Hospital affil-num=9 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Kurashiki Central Hospital affil-num=10 en-affil= kn-affil=Department of Gastroenterology, Tsuyama Central Hospital affil-num=11 en-affil= kn-affil=Department of Hepatology, Kagawa Prefectural Central Hospital affil-num=12 en-affil= kn-affil=Department of Hepatology, Kagawa Prefectural Central Hospital affil-num=13 en-affil= kn-affil=Department of Medicine, Mitoyo Central Hospital affil-num=14 en-affil= kn-affil=Department of Internal Medicine, Hiroshima City Hospital affil-num=15 en-affil= kn-affil=Department of Internal Medicine, Hiroshima City Hospital affil-num=16 en-affil= kn-affil=Department of Internal Medicine, Fukuyama City Hospital affil-num=17 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=18 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=entecavir kn-keyword=entecavir en-keyword=hepatitis B virus kn-keyword=hepatitis B virus en-keyword=lamivudine kn-keyword=lamivudine en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue= article-no= start-page=21721 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160217 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis en-subtitle= kn-subtitle= en-abstract= kn-abstract=　Although obesity is undoubtedly major risk for non-alcoholic steatohepatitis (NASH), the presence of lean NASH patients with normal body mass index has been recognized. Here, we report that the insufficiency of phosphatidylethanolamine N-methyltransferase (PEMT) is a risk for the lean NASH. The Pemt−/− mice fed high fat-high sucrose (HFHS) diet were protected from diet-induced obesity and diabetes, while they demonstrated prominent steatohepatitis and developed multiple liver tumors. Pemt exerted inhibitory effects on p53-driven transcription by forming the complex with clathrin heavy chain and p53, and Pemt−/− mice fed HFHS diet demonstrated prominent apoptosis of hepatocytes. Furthermore, hypermethylation and suppressed mRNA expression of F-box protein 31 and hepatocyte nuclear factor 4α resulted in the prominent activation of cyclin D1. PEMT mRNA expression in liver tissues of NASH patients was significantly lower than those with simple steatosis and we postulated the distinct clinical entity of lean NASH with insufficiency of PEMT activities. en-copyright= kn-copyright= en-aut-name=NakatsukaAtsuko en-aut-sei=Nakatsuka en-aut-mei=Atsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuyamaMakoto en-aut-sei=Matsuyama en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamaguchiSatoshi en-aut-sei=Yamaguchi en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KatayamaAkihiro en-aut-sei=Katayama en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=EguchiJun en-aut-sei=Eguchi en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurakamiKazutoshi en-aut-sei=Murakami en-aut-mei=Kazutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TeshigawaraSanae en-aut-sei=Teshigawara en-aut-mei=Sanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OgawaDaisuke en-aut-sei=Ogawa en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=WadaNozomu en-aut-sei=Wada en-aut-mei=Nozomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YasunakaTetsuya en-aut-sei=Yasunaka en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IkedaFusao en-aut-sei=Ikeda en-aut-mei=Fusao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TakakiAkinobu en-aut-sei=Takaki en-aut-mei=Akinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=WatanabeEijiro en-aut-sei=Watanabe en-aut-mei=Eijiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Shigei Medical Research Institute affil-num=3 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Diabetic Nephropathy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=10 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=11 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=12 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=13 en-affil= kn-affil=Dainippon Sumitomo Pharma affil-num=14 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=112 cd-vols= no-issue=39 article-no= start-page=E5401 end-page=E5410 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150929 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification of the VERNALIZATION 4 gene reveals the origin of spring growth habit in ancient wheats from South Asia en-subtitle= kn-subtitle= en-abstract= kn-abstract=Wheat varieties with a winter growth habit require long exposures to low temperatures (vernalization) to accelerate flowering. Natural variation in four vernalization genes regulating this requirement has favored wheat adaptation to different environments. The first three genes (VRN1–VRN3) have been cloned and characterized before. Here we show that the fourth gene, VRN-D4, originated by the insertion of a ∼290-kb region from chromosome arm 5AL into the proximal region of chromosome arm 5DS. The inserted 5AL region includes a copy of VRN-A1 that carries distinctive mutations in its coding and regulatory regions. Three lines of evidence confirmed that this gene is VRN-D4: it cosegregated with VRN-D4 in a high-density mapping population; it was expressed earlier than other VRN1 genes in the absence of vernalization; and induced mutations in this gene resulted in delayed flowering. VRN-D4 was found in most accessions of the ancient subspecies Triticum aestivum ssp. sphaerococcum from South Asia. This subspecies showed a significant reduction of genetic diversity and increased genetic differentiation in the centromeric region of chromosome 5D, suggesting that VRN-D4 likely contributed to local adaptation and was favored by positive selection. Three adjacent SNPs in a regulatory region of the VRN-D4 first intron disrupt the binding of GLYCINE-RICH RNA-BINDING PROTEIN 2 (TaGRP2), a known repressor of VRN1 expression. The same SNPs were identified in VRN-A1 alleles previously associated with reduced vernalization requirement. These alleles can be used to modulate vernalization requirements and to develop wheat varieties better adapted to different or changing environments. en-copyright= kn-copyright= en-aut-name=KippesNestor en-aut-sei=Kippes en-aut-mei=Nestor kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DebernardiJuan M. en-aut-sei=Debernardi en-aut-mei=Juan M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Vasquez-GrossHans A. en-aut-sei=Vasquez-Gross en-aut-mei=Hans A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkpinarBala A. en-aut-sei=Akpinar en-aut-mei=Bala A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=BudakHikment en-aut-sei=Budak en-aut-mei=Hikment kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KatoKenji en-aut-sei=Kato en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ChaoShiaoman en-aut-sei=Chao en-aut-mei=Shiaoman kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=AkhunovEduard en-aut-sei=Akhunov en-aut-mei=Eduard kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=DubcovskyJorge en-aut-sei=Dubcovsky en-aut-mei=Jorge kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Plant Sciences, University of California affil-num=2 en-affil= kn-affil=Department of Plant Sciences, University of California affil-num=3 en-affil= kn-affil=Department of Plant Sciences, University of California affil-num=4 en-affil= kn-affil=Faculty of Engineering and Natural Sciences, Sabanci University affil-num=5 en-affil= kn-affil=Faculty of Engineering and Natural Sciences, Sabanci University affil-num=6 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=7 en-affil= kn-affil=Biosciences Research Lab, US Department of Agriculture–Agricultural Research Service affil-num=8 en-affil= kn-affil=Department of Plant Pathology, Kansas State University affil-num=9 en-affil= kn-affil=Department of Plant Sciences, University of California en-keyword=wheat kn-keyword=wheat en-keyword=flowering kn-keyword=flowering en-keyword=vernalization kn-keyword=vernalization en-keyword=VRN1 kn-keyword=VRN1 en-keyword=Triticum aestivum ssp. sphaerococcum kn-keyword=Triticum aestivum ssp. sphaerococcum END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue= article-no= start-page=19742 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160128 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Molecular evolution of gas cavity in [NiFeSe] hydrogenases resurrected in silico en-subtitle= kn-subtitle= en-abstract= kn-abstract=Oxygen tolerance of selenium-containing [NiFeSe] hydrogenases (Hases) is attributable to the high reducing power of the selenocysteine residue, which sustains the bimetallic Ni–Fe catalytic center in the large subunit. Genes encoding [NiFeSe] Hases are inherited by few sulphate-reducing δ-proteobacteria globally distributed under various anoxic conditions. Ancestral sequences of [NiFeSe] Hases were elucidated and their three-dimensional structures were recreated in silico using homology modelling and molecular dynamic simulation, which suggested that deep gas channels gradually developed in [NiFeSe] Hases under absolute anaerobic conditions, whereas the enzyme remained as a sealed edifice under environmental conditions of a higher oxygen exposure risk. The development of a gas cavity appears to be driven by non-synonymous mutations, which cause subtle conformational changes locally and distantly, even including highly conserved sequence regions. en-copyright= kn-copyright= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsunekawaNaoki en-aut-sei=Tsunekawa en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NemotoMichiko en-aut-sei=Nemoto en-aut-mei=Michiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HiranoToshiyuki en-aut-sei=Hirano en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SatoFumitoshi en-aut-sei=Sato en-aut-mei=Fumitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=2 en-affil= kn-affil=Institute of Industrial Science, the University of Tokyo affil-num=3 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=5 en-affil= kn-affil=Institute of Industrial Science, the University of Tokyo affil-num=6 en-affil= kn-affil=Institute of Industrial Science, the University of Tokyo END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=16920 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=2015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Beneficial impact of Gpnmb and its significance as a biomarker in nonalcoholic steatohepatitis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease worldwide. Gpnmb is classified as a type 1 membrane protein and its soluble form is secreted by ADAM10-mediated cleavage. Gpnmb mRNA was found in the Kupffer cells and white adipose tissues (WATs) and its upregulation in obesity was recently found. Here, we generated aP2 promoter-driven Gpnmb transgenic (Tg) mice and the overexpression of Gpnmb ameliorated the fat accumulation and fibrosis of the liver in diet-induced obesity model. Soluble form of Gpnmb in sera was elevated in Gpnmb Tg mice and Gpnmb concentrated in hepatic macrophages and stellate cells interacted with calnexin, which resulted in the reduction of oxidative stress. In the patients with non-alcoholic steatohepatitis, serum soluble GPNMB concentrations were higher compared with the patients with simple steatosis. The GPNMB is a promising biomarker and therapeutic target for the development and progression of NAFLD in obesity. en-copyright= kn-copyright= en-aut-name=KatayamaAkihiro en-aut-sei=Katayama en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakatsukaAtsuko en-aut-sei=Nakatsuka en-aut-mei=Atsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=EguchiJun en-aut-sei=Eguchi en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiKazutoshi en-aut-sei=Murakami en-aut-mei=Kazutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TeshigawaraSanae en-aut-sei=Teshigawara en-aut-mei=Sanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KanzakiMotoko en-aut-sei=Kanzaki en-aut-mei=Motoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NunoueTomokazu en-aut-sei=Nunoue en-aut-mei=Tomokazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HidaKazuyuki en-aut-sei=Hida en-aut-mei=Kazuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=WadaNozomu en-aut-sei=Wada en-aut-mei=Nozomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YasunakaTetsuya en-aut-sei=Yasunaka en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IkedaFusao en-aut-sei=Ikeda en-aut-mei=Fusao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TakakiAkinobu en-aut-sei=Takaki en-aut-mei=Akinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=YamamotoKazuhide en-aut-sei=Yamamoto en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=KiyonariHiroshi en-aut-sei=Kiyonari en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=MakinoHirofumi en-aut-sei=Makino en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= affil-num=1 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Diabetes and Metabolism, National Hospital Organization Okayama Medical center affil-num=9 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=10 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=11 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=12 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=13 en-affil= kn-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=14 en-affil= kn-affil=Animal Resource Development Unit affil-num=15 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=16 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=12570 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=2015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Metamaterial Absorbers for Infrared Detection of Molecular Self-Assembled Monolayers en-subtitle= kn-subtitle= en-abstract= kn-abstract=The emerging field of plasmonic metamaterials has introduced new degree of freedom to manipulate optical field from nano to macroscopic scale, offering an attractive platform for sensing applications. So far, metamaterial sensor concepts, however, have focused on hot-spot engineering to improve the near-field enhancement, rather than fully exploiting tailored material properties. Here, we present a novel spectroscopic technique based on the metamaterial infrared (IR) absorber allowing for a low-background detection scheme as well as significant plasmonic enhancement. Specifically, we experimentally demonstrate the resonant coupling of plasmonic modes of a metamaterial absorber and IR vibrational modes of a molecular self-assembled monolayer. The metamaterial consisting of an array of Au/MgF2/Au structures exhibits an anomalous absorption at ~3000 cm−1, which spectrally overlaps with C-H stretching vibrational modes. Symmetric/asymmetric C-H stretching modes of a 16-Mercaptohexadecanoic acid monolayer are clearly observed as Fano-like anti-resonance peaks within a broad plasmonic absorption of the metamaterial. Spectral analysis using Fano line-shape fitting reveals the underlying resonant interference in plasmon-molecular coupled systems. Our metamaterial approach achieves the attomole sensitivity with a large signal-to-noise ratio in the far-field measurement, thus may open up new avenues for realizing ultrasensitive IR inspection technologies. en-copyright= kn-copyright= en-aut-name=IshikawaAtsushi en-aut-sei=Ishikawa en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaTakuo en-aut-sei=Tanaka en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Electrical and Electronic Engineering, Okayama University affil-num=2 en-affil= kn-affil=Metamaterials Laboratory, RIKEN END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=11468 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=2015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro en-subtitle= kn-subtitle= en-abstract= kn-abstract=In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication. en-copyright= kn-copyright= en-aut-name=TaketaHiroaki en-aut-sei=Taketa en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=GulsanAra Sathi en-aut-sei=Gulsan en-aut-mei=Ara Sathi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MahmoudFarahat en-aut-sei=Mahmoud en-aut-mei=Farahat kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KaziAnisur Rahman en-aut-sei=Kazi en-aut-mei=Anisur Rahman kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SakaiTakayoshi en-aut-sei=Sakai en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HiranoYoshiaki en-aut-sei=Hirano en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ToriiYasuhiro en-aut-sei=Torii en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MatsumotoTakuya en-aut-sei=Matsumoto en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=2 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=3 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=4 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=5 en-affil= kn-affil=Department of Oral-Facial Disorders, Osaka University affil-num=6 en-affil= kn-affil=Department of Chemical Engineering, Kansai University affil-num=7 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=8 en-affil= kn-affil=Department of Biomaterials, Okayama University affil-num=9 en-affil= kn-affil=Department of Biomaterials, Okayama University END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=3932 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140529 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=TRPV2 is critical for the maintenance of cardiac structure and function in mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=The heart has a dynamic compensatory mechanism for haemodynamic stress. However, the molecular details of how mechanical forces are transduced in the heart are unclear. Here we show that the transient receptor potential, vanilloid family type 2 (TRPV2) cation channel is critical for the maintenance of cardiac structure and function. Within 4 days of eliminating TRPV2 from hearts of the adult mice, cardiac function declines severely, with disorganization of the intercalated discs that support mechanical coupling with neighbouring myocytes and myocardial conduction defects. After 9 days, cell shortening and Ca2+ handling by single myocytes are impaired in TRPV2-deficient hearts. TRPV2-deficient neonatal cardiomyocytes form no intercalated discs and show no extracellular Ca2+-dependent intracellular Ca2+ increase and insulin-like growth factor (IGF-1) secretion in response to stretch stimulation. We further demonstrate that IGF-1 receptor/PI3K/Akt pathway signalling is significantly downregulated in TRPV2-deficient hearts, and that IGF-1 administration partially prevents chamber dilation and impairment in cardiac pump function in these hearts. Our results improve our understanding of the molecular processes underlying the maintenance of cardiac structure and function. en-copyright= kn-copyright= en-aut-name=KatanosakaYuki en-aut-sei=Katanosaka en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IwasakiKeiichiro en-aut-sei=Iwasaki en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UjiharaYoshihiro en-aut-sei=Ujihara en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakatsuSatomi en-aut-sei=Takatsu en-aut-mei=Satomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishitsujiKoki en-aut-sei=Nishitsuji en-aut-mei=Koki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KanagawaMotoi en-aut-sei=Kanagawa en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SudoAtsushi en-aut-sei=Sudo en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TodaTatsushi en-aut-sei=Toda en-aut-mei=Tatsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KatanosakaKimiaki en-aut-sei=Katanosaka en-aut-mei=Kimiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=MohriSatoshi en-aut-sei=Mohri en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=NaruseKeiji en-aut-sei=Naruse en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=4 en-affil= kn-affil=Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=5 en-affil= kn-affil=Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=6 en-affil= kn-affil=Division of Neurology/Molecular Brain Science, Kobe University Graduate School of Medicine affil-num=7 en-affil= kn-affil=Division of Neurology/Molecular Brain Science, Kobe University Graduate School of Medicine affil-num=8 en-affil= kn-affil=Division of Neurology/Molecular Brain Science, Kobe University Graduate School of Medicine affil-num=9 en-affil= kn-affil=Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University affil-num=10 en-affil= kn-affil=Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=11 en-affil= kn-affil=Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=13539 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=2015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A subset of ocular adnexal marginal zone lymphomas may arise in association with IgG4-related disease en-subtitle= kn-subtitle= en-abstract= kn-abstract=We previously suggested a relationship between ocular immunoglobulin (Ig)G4-related disease (IgG4-RD) and marginal zone lymphomas (MZLs). However, the cytokine background associated with these disorders and whether it differs between ocular adnexal MZLs with (IgG4-associated MZL) and without (IgG4-negative MZL) numerous IgG4+ plasma cells are unknown. In this study, we identified the mRNA expression pattern of Th2 and regulatory T-cell (Treg) cytokines in IgG4-RD and in IgG4-associated MZL and IgG4-negative MZL using real-time polymerase chain reaction analysis. Ocular IgG4-RD and IgG4-associated MZL exhibited significantly higher expression ratios of interleukin (IL)-4/β-actin, IL-10/β-actin, IL-13/β-actin, transforming growth factor (TGF) β1/β-actin, and FOXP3/β-actin than did IgG4-negative MZL (p < 0.05). This finding further supports our prior observations that a significant subset of ocular MZLs arises in the setting of IgG4-RD. Furthermore, the presence of a different inflammatory background in IgG4-negative MZLs suggests that IgG4-associated MZLs may have a different pathogenesis. en-copyright= kn-copyright= en-aut-name=KyotaroOhno en-aut-sei=Kyotaro en-aut-mei=Ohno kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YasuharuSato en-aut-sei=Yasuharu en-aut-mei=Sato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Koh-ichiOhshima en-aut-sei=Koh-ichi en-aut-mei=Ohshima kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KatsuyoshiTakata en-aut-sei=Katsuyoshi en-aut-mei=Takata kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TomokoMiyata-Takata en-aut-sei=Tomoko en-aut-mei=Miyata-Takata kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MaiTakeuchi en-aut-sei=Mai en-aut-mei=Takeuchi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YukaGion en-aut-sei=Yuka en-aut-mei=Gion kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TomoyasuTachibana en-aut-sei=Tomoyasu en-aut-mei=Tachibana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YorihisaOrita en-aut-sei=Yorihisa en-aut-mei=Orita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ToshihiroIto en-aut-sei=Toshihiro en-aut-mei=Ito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=StevenH. Swerdlow en-aut-sei=Steven en-aut-mei=H. Swerdlow kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TadashiYoshino en-aut-sei=Tadashi en-aut-mei=Yoshino kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil= kn-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Ophthalmology, National Hospital Organization Okayama Medical Center affil-num=4 en-affil= kn-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Otolaryngology, Himeji Red Cross Hospital affil-num=9 en-affil= kn-affil=Department of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=10 en-affil= kn-affil=Department of Immunology, Nara Medical University affil-num=11 en-affil= kn-affil=Department of Pathology, Division of Hematopathology, University of Pittsburgh School of Medicine affil-num=12 en-affil= kn-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=14812 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=2015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Regulation of the unfolded protein response via S-nitrosylation of sensors of endoplasmic reticulum stress en-subtitle= kn-subtitle= en-abstract= kn-abstract=Protein S-nitrosylation modulates important cellular processes, including neurotransmission, vasodilation, proliferation, and apoptosis in various cell types. We have previously reported that protein disulfide isomerase (PDI) is S-nitrosylated in brains of patients with sporadic neurodegenerative diseases. This modification inhibits PDI enzymatic activity and consequently leads to the accumulation of unfolded/misfolded proteins in the endoplasmic reticulum (ER) lumen. Here, we describe S-nitrosylation of additional ER pathways that affect the unfolded protein response (UPR) in cell-based models of Parkinson's disease (PD). We demonstrate that nitric oxide (NO) can S-nitrosylate the ER stress sensors IRE1α and PERK. While S-nitrosylation of IRE1α inhibited its ribonuclease activity, S-nitrosylation of PERK activated its kinase activity and downstream phosphorylation/inactivation or eIF2α. Site-directed mutagenesis of IRE1α(Cys931) prevented S-nitrosylation and inhibition of its ribonuclease activity, indicating that Cys931 is the predominant site of S-nitrosylation. Importantly, cells overexpressing mutant IRE1α(C931S) were resistant to NO-induced damage. Our findings show that nitrosative stress leads to dysfunctional ER stress signaling, thus contributing to neuronal cell death. en-copyright= kn-copyright= en-aut-name=RyosukeNakato en-aut-sei=Ryosuke en-aut-mei=Nakato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YuOhkubo en-aut-sei=Yu en-aut-mei=Ohkubo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkariKonishi en-aut-sei=Akari en-aut-mei=Konishi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MariShibata en-aut-sei=Mari en-aut-mei=Shibata kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YukiKaneko en-aut-sei=Yuki en-aut-mei=Kaneko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakaoIwawaki en-aut-sei=Takao en-aut-mei=Iwawaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TomohiroNakamura en-aut-sei=Tomohiro en-aut-mei=Nakamura kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=Stuart A.Lipton en-aut-sei=Stuart A. en-aut-mei=Lipton kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TakashiUehara en-aut-sei=Takashi en-aut-mei=Uehara kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Medicinal Pharmacology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Department of Medicinal Pharmacology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Department of Medicinal Pharmacology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University affil-num=4 en-affil= kn-affil=Department of Medicinal Pharmacology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University affil-num=5 en-affil= kn-affil=Department of Medicinal Pharmacology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University affil-num=6 en-affil= kn-affil=Iwawaki laboratory, Education and Research Support Center, Graduate School of Medicine, Gunma University affil-num=7 en-affil= kn-affil=Neuroscience and Aging Research Center, Sanford-Burnham-Prebys Medical Discovery Institute affil-num=8 en-affil= kn-affil=Neuroscience and Aging Research Center, Sanford-Burnham-Prebys Medical Discovery Institute affil-num=9 en-affil= kn-affil=Department of Medicinal Pharmacology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150105 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress. en-copyright= kn-copyright= en-aut-name=MiyajiTakaaki en-aut-sei=Miyaji en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KuromoriTakashi en-aut-sei=Kuromori en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakeuchiYu en-aut-sei=Takeuchi en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamajiNaoki en-aut-sei=Yamaji en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YokoshoKengo en-aut-sei=Yokosho en-aut-mei=Kengo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShimazawaAtsushi en-aut-sei=Shimazawa en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SugimotoEriko en-aut-sei=Sugimoto en-aut-mei=Eriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OmoteHiroshi en-aut-sei=Omote en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MaJian Feng en-aut-sei=Ma en-aut-mei=Jian Feng kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ShinozakiKazuo en-aut-sei=Shinozaki en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=MoriyamaYoshinori en-aut-sei=Moriyama en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Advanced Science Research Center, Okayama University affil-num=2 en-affil= kn-affil=Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science affil-num=3 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=5 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=6 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science affil-num=8 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=10 en-affil= kn-affil=Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science affil-num=11 en-affil= kn-affil=Advanced Science Research Center, Okayama University END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering en-subtitle= kn-subtitle= en-abstract= kn-abstract=Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37 degrees C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury. en-copyright= kn-copyright= en-aut-name=MazakiTetsuro en-aut-sei=Mazaki en-aut-mei=Tetsuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShiozakiYasuyuki en-aut-sei=Shiozaki en-aut-mei=Yasuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamaneKentaro en-aut-sei=Yamane en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshidaAki en-aut-sei=Yoshida en-aut-mei=Aki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakamuraMariko en-aut-sei=Nakamura en-aut-mei=Mariko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YoshidaYasuhiro en-aut-sei=Yoshida en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ZhouDi en-aut-sei=Zhou en-aut-mei=Di kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KitajimaTakashi en-aut-sei=Kitajima en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TanakaMasato en-aut-sei=Tanaka en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ItoYoshihiro en-aut-sei=Ito en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=MatsukawaAkihiro en-aut-sei=Matsukawa en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg affil-num=2 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg affil-num=3 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg affil-num=4 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg affil-num=5 en-affil= kn-affil=Kibi Int Univ Jr Coll, Dept Hlth & Welf Program affil-num=6 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biomat affil-num=7 en-affil= kn-affil=RIKEN, Nano Med Engn Lab affil-num=8 en-affil= kn-affil=RIKEN, Nano Med Engn Lab affil-num=9 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg affil-num=10 en-affil= kn-affil=RIKEN, Nano Med Engn Lab affil-num=11 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg affil-num=12 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pathol & Expt Med END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140813 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy en-subtitle= kn-subtitle= en-abstract= kn-abstract=Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy. en-copyright= kn-copyright= en-aut-name=OshimaMasamitsu en-aut-sei=Oshima en-aut-mei=Masamitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InoueKaoru en-aut-sei=Inoue en-aut-mei=Kaoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakajimaKei en-aut-sei=Nakajima en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TachikawaTetsuhiko en-aut-sei=Tachikawa en-aut-mei=Tetsuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamazakiHiromichi en-aut-sei=Yamazaki en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IsobeTomohide en-aut-sei=Isobe en-aut-mei=Tomohide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SugawaraAyaka en-aut-sei=Sugawara en-aut-mei=Ayaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OgawaMiho en-aut-sei=Ogawa en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TanakaChie en-aut-sei=Tanaka en-aut-mei=Chie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SaitoMasahiro en-aut-sei=Saito en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KasugaiShohei en-aut-sei=Kasugai en-aut-mei=Shohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=Takano-YamamotoTeruko en-aut-sei=Takano-Yamamoto en-aut-mei=Teruko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=InoueTakashi en-aut-sei=Inoue en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=TezukaKatsunari en-aut-sei=Tezuka en-aut-mei=Katsunari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=KubokiTakuo en-aut-sei=Kuboki en-aut-mei=Takuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=YamaguchiAkira en-aut-sei=Yamaguchi en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=TsujiTakashi en-aut-sei=Tsuji en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= affil-num=1 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol affil-num=2 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=3 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=4 en-affil= kn-affil=Showa Univ, Sch Dent, Dept Oral Pathol affil-num=5 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=6 en-affil= kn-affil=Showa Univ, Sch Dent, Dept Oral Pathol affil-num=7 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=8 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol affil-num=9 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=10 en-affil= kn-affil=Tokyo Univ Sci, Grad Sch Ind Sci & Technol, Dept Biol Sci & Technol affil-num=11 en-affil= kn-affil=Tokyo Med & Dent Univ, Grad Sch, Sect Oral Implantol & Regenerat Dent Med affil-num=12 en-affil= kn-affil=Tohoku Univ, Grad Sch Dent, Div Orthodont & Dentofacial Orthoped affil-num=13 en-affil= kn-affil=Tokyo Dent Coll, Dept Clin Pathophysiol affil-num=14 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol affil-num=15 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Oral Rehabil & Regenerat Med affil-num=16 en-affil= kn-affil=Tokyo Med & Dent Univ, Grad Sch, Dept Oral Restitut, Sect Oral Pathol affil-num=17 en-affil= kn-affil=Tokyo Univ Sci, Res Inst Sci & Technol END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20141030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification of a mammalian vesicular polyamine transporter en-subtitle= kn-subtitle= en-abstract= kn-abstract=Spermine and spermidine act as neuromodulators upon binding to the extracellular site(s) of various ionotropic receptors, such as N-methyl-d-aspartate receptors. To gain access to the receptors, polyamines synthesized in neurons and astrocytes are stored in secretory vesicles and released upon depolarization. Although vesicular storage is mediated in an ATP-dependent, reserpine-sensitive fashion, the transporter responsible for this process remains unknown. SLC18B1 is the fourth member of the SLC18 transporter family, which includes vesicular monoamine transporters and vesicular acetylcholine transporter. Proteoliposomes containing purified human SLC18B1 protein actively transport spermine and spermidine by exchange of H+. SLC18B1 protein is predominantly expressed in the hippocampus and is associated with vesicles in astrocytes. SLC18B1 gene knockdown decreased both SLC18B1 protein and spermine/spermidine contents in astrocytes. These results indicated that SLC18B1 encodes a vesicular polyamine transporter (VPAT). en-copyright= kn-copyright= en-aut-name=HiasaMiki en-aut-sei=Hiasa en-aut-mei=Miki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyajiTakaaki en-aut-sei=Miyaji en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HarunaYuka en-aut-sei=Haruna en-aut-mei=Yuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakeuchiTomoya en-aut-sei=Takeuchi en-aut-mei=Tomoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HaradaYuika en-aut-sei=Harada en-aut-mei=Yuika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriyamaSawako en-aut-sei=Moriyama en-aut-mei=Sawako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YamamotoAkitsugu en-aut-sei=Yamamoto en-aut-mei=Akitsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OmoteHiroshi en-aut-sei=Omote en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MoriyamaYoshinori en-aut-sei=Moriyama en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Advanced Science Research Center, Okayama University affil-num=3 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Faculty of Bioscience, Nagahama Institute of Bio-science and Technology affil-num=8 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140616 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Efficient DNA Fingerprinting Based on the Targeted Sequencing of Active Retrotransposon Insertion Sites Using a Bench-Top High-Throughput Sequencing Platform en-subtitle= kn-subtitle= en-abstract= kn-abstract=In many crop species, DNA fingerprinting is required for the precise identification of cultivars to protect the rights of breeders. Many families of retrotransposons have multiple copies throughout the eukaryotic genome and their integrated copies are inherited genetically. Thus, their insertion polymorphisms among cultivars are useful for DNA fingerprinting. In this study, we conducted a DNA fingerprinting based on the insertion polymorphisms of active retrotransposon families (Rtsp-1 and LIb) in sweet potato. Using 38 cultivars, we identified 2024 insertion sites in the two families with an Illumina MiSeq sequencing platform. Of these insertion sites, 91.4% appeared to be polymorphic among the cultivars and 376 cultivar-specific insertion sites were identified, which were converted directly into cultivar-specific sequence-characterized amplified region (SCAR) markers. A phylogenetic tree was constructed using these insertion sites, which corresponded well with known pedigree information, thereby indicating their suitability for genetic diversity studies. Thus, the genome-wide comparative analysis of active retrotransposon insertion sites using the bench-top MiSeq sequencing platform is highly effective for DNA fingerprinting without any requirement for whole genome sequence information. This approach may facilitate the development of practical polymerase chain reaction-based cultivar diagnostic system and could also be applied to the determination of genetic relationships. en-copyright= kn-copyright= en-aut-name=MondenYuki en-aut-sei=Monden en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoAyaka en-aut-sei=Yamamoto en-aut-mei=Ayaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShindoAkiko en-aut-sei=Shindo en-aut-mei=Akiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TaharaMakoto en-aut-sei=Tahara en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=2 en-affil= kn-affil=Faculty of Agriculture, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University en-keyword=DNA fingerprinting kn-keyword=DNA fingerprinting en-keyword=high-throughput sequencing kn-keyword=high-throughput sequencing en-keyword=molecular marker kn-keyword=molecular marker en-keyword=retrotransposon kn-keyword=retrotransposon en-keyword=sweet potato kn-keyword=sweet potato END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=5 article-no= start-page=245 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140625 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Efficient screening of long terminal repeat retrotransposons that show high insertion polymorphism via high-throughput sequencing of the primer binding site en-subtitle= kn-subtitle= en-abstract= kn-abstract=Retrotransposons have been used frequently for the development of molecular markers by using their insertion polymorphisms among cultivars, because multiple copies of these elements are dispersed throughout the genome and inserted copies are inherited genetically. Although a large number of long terminal repeat (LTR) retrotransposon families exist in the higher eukaryotic genomes, the identification of families that show high insertion polymorphism has been challenging. Here, we performed an efficient screening of these retrotransposon families using an Illumina HiSeq2000 sequencing platform with comprehensive LTR library construction based on the primer binding site (PBS), which is located adjacent to the 5′ LTR and has a motif that is universal and conserved among LTR retrotransposon families. The paired-end sequencing library of the fragments containing a large number of LTR sequences and their insertion sites was sequenced for seven strawberry (Fragaria × ananassa Duchesne) cultivars and one diploid wild species (Fragaria vesca L.). Among them, we screened 24 families with a “unique” insertion site that appeared only in one cultivar and not in any others, assuming that this type of insertion should have occurred quite recently. Finally, we confirmed experimentally the selected LTR families showed high insertion polymorphisms among closely related cultivars. en-copyright= kn-copyright= en-aut-name=MondenYuki en-aut-sei=Monden en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujiiNobuyuki en-aut-sei=Fujii en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamaguchiKentaro en-aut-sei=Yamaguchi en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IkeoKazuho en-aut-sei=Ikeo en-aut-mei=Kazuho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakazawaYoshiko en-aut-sei=Nakazawa en-aut-mei=Yoshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WakiTakamitsu en-aut-sei=Waki en-aut-mei=Takamitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HirashimaKeita en-aut-sei=Hirashima en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UchimuraYosuke en-aut-sei=Uchimura en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TaharaMakoto en-aut-sei=Tahara en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=2 en-affil= kn-affil=Center for Information Biology, National Institute of Genetics Research Organization of Information and Systems affil-num=3 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University affil-num=4 en-affil= kn-affil=Center for Information Biology, National Institute of Genetics Research Organization of Information and Systems affil-num=5 en-affil= kn-affil=Biotechology Division, Tochigi Prefectural Agricultural Experiment Station affil-num=6 en-affil= kn-affil=Biotechology Division, Tochigi Prefectural Agricultural Experiment Station affil-num=7 en-affil= kn-affil=Department of Research Plan and Strategy, Fukuoka Agricultural Research Center affil-num=8 en-affil= kn-affil=Department of Research Plan and Strategy, Fukuoka Agricultural Research Center affil-num=9 en-affil= kn-affil=Graduate School of Environmental and Life Science, Okayama University en-keyword=retrotransposon kn-keyword=retrotransposon en-keyword=primer binding site kn-keyword=primer binding site en-keyword=high-throughput sequencing kn-keyword=high-throughput sequencing en-keyword=polymorphism kn-keyword=polymorphism en-keyword=molecular markers kn-keyword=molecular markers END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of hydrogen-rich water on aging periodontal tissues in rats en-subtitle= kn-subtitle= en-abstract= kn-abstract=Oxidative damage is involved in age-related inflammatory reactions. The anti-oxidative effects of hydrogen-rich water suppress oxidative damage, which may aid in inhibiting age-related inflammatory reactions. We investigated the effects of drinking hydrogen-rich water on aging periodontal tissues in healthy rats. Four-month-old male Fischer 344 rats (n = 12) were divided into two groups: the experimental group (hydrogen-rich water treatment) and the control group (distilled water treatment). The rats consumed hydrogen-rich water or distilled water until 16 months of age. The experimental group exhibited lower periodontal oxidative damage at 16 months of age than the control group. Although protein expression of interleukin-1 beta did not differ, gene expression of Nod-like receptor protein 3 inflammasomes was activated in periodontal tissues from the experimental group as compared with the control group. Drinking hydrogen-rich water is proposed to have anti-aging effects on periodontal oxidative damage, but not on inflammatory reactions in healthy rats. en-copyright= kn-copyright= en-aut-name=TomofujiTakaaki en-aut-sei=Tomofuji en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawabataYuya en-aut-sei=Kawabata en-aut-mei=Yuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KasuyamaKenta en-aut-sei=Kasuyama en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=EndoYasumasa en-aut-sei=Endo en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YonedaToshiki en-aut-sei=Yoneda en-aut-mei=Toshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YamaneMayu en-aut-sei=Yamane en-aut-mei=Mayu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AzumaTetsuji en-aut-sei=Azuma en-aut-mei=Tetsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=EkuniDaisuke en-aut-sei=Ekuni en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MoritaManabu en-aut-sei=Morita en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=2 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=3 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=4 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=5 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=6 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=7 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=8 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent affil-num=9 en-affil= kn-affil=Okayama Univ, Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent END start-ver=1.4 cd-journal=joma no-vol=20 cd-vols= no-issue=19 article-no= start-page=4205 end-page=4215 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20091001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=mTrs130 Is a Component of a Mammalian TRAPPII Complex, a Rab1 GEF That Binds to COPI-coated Vesicles en-subtitle= kn-subtitle= en-abstract= kn-abstract=The GTPase Rab1 regulates endoplasmic reticulum-Golgi and early Golgi traffic. The guanine nucleotide exchange factor (GEF) or factors that activate Rab1 at these stages of the secretory pathway are currently unknown. Trs130p is a subunit of the yeast TRAPPII (transport protein particle II) complex, a multisubunit tethering complex that is a GEF for the Rab1 homologue Ypt1p. Here, we show that mammalian Trs130 (mTrs130) is a component of an analogous TRAPP complex in mammalian cells, and we describe for the first time the role that this complex plays in membrane traffic. mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not Golgi cisternae, and it specifically activates Rab1. In addition, we find that mTRAPPII binds to γ1COP, a COPI coat adaptor subunit. The depletion of mTrs130 by short hairpin RNA leads to an increase of vesicles in the vicinity of the Golgi and the accumulation of cargo in an early Golgi compartment. We propose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes. en-copyright= kn-copyright= en-aut-name=YamasakiAkinori en-aut-sei=Yamasaki en-aut-mei=Akinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MenonShekar en-aut-sei=Menon en-aut-mei=Shekar kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YuSidney en-aut-sei=Yu en-aut-mei=Sidney kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=BarrowmanJemima en-aut-sei=Barrowman en-aut-mei=Jemima kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MeerlooTimo en-aut-sei=Meerloo en-aut-mei=Timo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OorschotViola en-aut-sei=Oorschot en-aut-mei=Viola kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KlumpermanJudith en-aut-sei=Klumperman en-aut-mei=Judith kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SatohAyano en-aut-sei=Satoh en-aut-mei=Ayano kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=Ferro-NovickSusan en-aut-sei=Ferro-Novick en-aut-mei=Susan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine affil-num=2 en-affil= kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine affil-num=3 en-affil= kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine affil-num=4 en-affil= kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine affil-num=5 en-affil= kn-affil=Department of Cellular and Molecular Medicine, University of California at San Diego affil-num=6 en-affil= kn-affil=Cell Microscopy Center, Department of Cell Biology, University Medical Center Utrecht affil-num=7 en-affil= kn-affil=Cell Microscopy Center, Department of Cell Biology, University Medical Center Utrecht affil-num=8 en-affil= kn-affil=Department of Cell Biology, Yale University School of Medicine affil-num=9 en-affil= kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=1 article-no= start-page=65 end-page=76 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ON A GENERALIZATION OF CQF-3′ MODULES AND COHEREDITARY TORSION THEORIES en-subtitle= kn-subtitle= en-abstract= kn-abstract=Throughout this paper we assume that R is a right perfect ring with identity and let Mod-R be the category of right R-modules. Let M be a right R-module. We denote by 0 → K(M) → P(M) → M → 0 the projective cover of M. M is called a CQF-3′ module, if P(M) is M-generated, that is, P(M) is isomorphic to a homomorphic image of a direct sum ⊕M of some copies of M. A subfunctor of the identity functor of Mod-R is called a preradical. For a preradical σ, Tσ := {M ∈ Mod-R : σ(M) = M} is called the class of σ-torsion right R-modules, and Fσ := {M ∈ Mod-R : σ(M) = 0} is called the class of σ-torsionfree right R-modules. A right R-module M is called σ-projective if the functor HomR(M,−) preserves the exactness for any exact sequence 0 → A → B → C → 0 with A ∈ Fσ. We put Pσ(M) = P(M)/σ(K(M)) for a module M. We call a right R-module M a σ-CQF-3′ module if Pσ(M) is M-generated. In this paper, we characterize σ-CQF-3′ modules and give some related facts. en-copyright= kn-copyright= en-aut-name=TakehanaYasuhiko en-aut-sei=Takehana en-aut-mei=Yasuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=General Education Hakodate National College of Technology en-keyword=QF-3′ kn-keyword=QF-3′ en-keyword=cohereditary kn-keyword=cohereditary END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=1 article-no= start-page=53 end-page=63 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ON A GENERALIZATION OF QF-3′ MODULES AND HEREDITARY TORSION THEORIES en-subtitle= kn-subtitle= en-abstract= kn-abstract=Let R be a ring with identity, and let Mod-R be the category of right R-modules. Let M be a right R-module. We denote by E(M) the injective hull of M. M is called QF-3′ module, if E(M) is M-torsionless, that is, E(M) is isomorphic to a submodule of a direct product ΠM of some copies of M. A subfunctor of the identity functor of Mod-R is called a preradical. For a preradical σ, Tσ := {M ∈ Mod-R : σ(M) = M} is the class of σ-torsion right R-modules, and Fσ := {M ∈ Mod-R : σ(M) = 0} is the class of σ-torsionfree right R-modules. A right R-module M is called σ-injective if the functor HomR(−,M) preserves the exactness for any exact sequence 0 → A → B → C → 0 with C ∈ Tσ. A right R-module M is called σ-QF-3′ module if Eσ(M) is M-torsionless, where Eσ(M) is defined by Eσ(M)/M := σ(E(M)/M). In this paper, we characterize σ-QF-3′ modules and give some related facts. en-copyright= kn-copyright= en-aut-name=TakehanaYasuhiko en-aut-sei=Takehana en-aut-mei=Yasuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=General Education Hakodate National College of Technology en-keyword=QF-3′ kn-keyword=QF-3′ en-keyword=hereditary kn-keyword=hereditary END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=3 article-no= start-page=1348 end-page=1356 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110315 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A MAC-address Relaying NAT Router for Host Identification from Outside of Internal Network kn-title=内部ネットワーク上のホストを外部から識別するためのMACアドレス中継型NATルータ en-subtitle= kn-subtitle= en-abstract=As an alleviation method against IPv4 address exhaustion problem, NAT (Network Address Translation) has been commonly used. Since NAT allows many internal hosts to share one single global IP address, it can save the number of required global IP addresses. However, with NAT, each internal host cannot be identified from the external network. Consequently, if access control system on external network would permit network access from one internal host, it automatically would permit all network access from any other internal hosts as well, for example. In this paper, we propose a NAT router with MAC address relaying function that copies the source MAC address of receiving frames sent by internal hosts into frames sent to the external network since source MAC addresses, which are the sender identifiers in data link layer, are basically unused except for MAC address learning function of layer 2 switches. According to the results of experiments, we confirmed that the prototype NAT router with MAC address relaying function allows access to external networks by internal hosts to be controlled individually based on MAC address and obtains high throughput as well. kn-abstract=IPv4アドレスの枯渇問題の軽減策の1つとして，NAT（Network Address Translation）がある．NATは複数の内部ホストが1つのグローバルIPアドレスを共用できるため，必要なグローバルIPアドレスの数を節約できる．しかし，外部ネットワーク側では個々の内部ホストを識別できないため，たとえば外部ネットワーク側でアクセス制御を行うと，1台の内部ホストが外部ネットワークに対するアクセス許可を受けただけで他の内部ホストまで外部ネットワークにアクセス可能な状態になるなどの問題が生じる．そこで，本論文ではデータリンク層での送信元識別子である送信元MACアドレスが基本的にはレイヤ2機器のMACアドレス学習機能にしか使われていない点に着目し，内部ホストから送信されたフレームに含まれる送信元MACアドレスをそのまま外部ネットワーク側に中継する機能を持つNATルータを提案する．本提案に基づいて試作したNATルータを評価した結果，MACアドレスに基づいて内部ホストを個別にアクセス制御でき，また十分なスループットが得られることを確認した． en-copyright= kn-copyright= en-aut-name=YamaiNariyoshi en-aut-sei=Yamai en-aut-mei=Nariyoshi kn-aut-name=山井成良 kn-aut-sei=山井 kn-aut-mei=成良 aut-affil-num=1 ORCID= en-aut-name=MurakamiRyo en-aut-sei=Murakami en-aut-mei=Ryo kn-aut-name=村上亮 kn-aut-sei=村上 kn-aut-mei=亮 aut-affil-num=2 ORCID= en-aut-name=OkayamaKiyohiko en-aut-sei=Okayama en-aut-mei=Kiyohiko kn-aut-name=岡山聖彦 kn-aut-sei=岡山 kn-aut-mei=聖彦 aut-affil-num=3 ORCID= en-aut-name=NakamuraMotonori en-aut-sei=Nakamura en-aut-mei=Motonori kn-aut-name=中村素典 kn-aut-sei=中村 kn-aut-mei=素典 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学情報統括センター affil-num=2 en-affil= kn-affil=岡山大学大学院自然科学研究科 affil-num=3 en-affil= kn-affil=岡山大学情報統括センター affil-num=4 en-affil= kn-affil=国立情報学研究所 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=423 end-page=435 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Detection of human sequence related to the squirrel monkey retrovirus kn-title=ヒトゲノムDNA中のリスザルレトロウイルス関連遺伝子の検出 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Squirrel monkey retrovirus (SMRV) is an endogenous type D retrovirus of the squirrel monkey, a New World primate. Southern hybridization with cloned SMRV-H revealed that 3040 copies of SMRV proviral DNA are present in the squirrel monkey genome and the majority have almost the same physical map as that of the cloned SMRV-H. SMRV-related sequences in the human genome were sought using the same method with various cloned SMRV-H DNA fragments under conditions of relaxed stringency. The discrete restriction fragments were frequently detected in the DNA from normal humans with the LTR and parts of gag and env as probes. Since SMRV LTR has very little homology with the LTRs of other retroviruses, the fragments detected with the LTR probe were characterized as SMRV-related human sequences. SMRV LTR-related sequences were also detected in the African green monkey and chicken, but not in the salmon, mouse, or dog. In conclusion, SMRV-related sequences are present in human DNA, and some of them might represent endogenous retroviral sequences of human DNA. en-copyright= kn-copyright= en-aut-name=AsonumaHirohiko en-aut-sei=Asonuma en-aut-mei=Hirohiko kn-aut-name=阿曽沼裕彦 kn-aut-sei=阿曽沼 kn-aut-mei=裕彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=ヒトDNA kn-keyword=ヒトDNA en-keyword=内在性レトロウイルス kn-keyword=内在性レトロウイルス en-keyword=リスザルレトロウイルス kn-keyword=リスザルレトロウイルス en-keyword=サザンハイブリダイゼーション kn-keyword=サザンハイブリダイゼーション END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=1-2 article-no= start-page=155 end-page=166 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Detection of gene expression in cells trasfected with recombinant DNA by in situ hybridization kn-title=遺伝子導入細胞における遺伝子発現のin situハイブリダイゼイションによる検出 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Expression of mRNA in monolayer cells transfected with recombinant DNA was demonstrated by in situ hybridization with biotin- or (32)P-labeled probes. The biotin-labeled probe and hybrid detection system used in this study could detect 1 pg of target DNA and a single copy gene in mammalian cells using filter hybridization techniques. Several conditions of in situ hybridization were optimized with neomycin resistant (neo(r)) cells, which constitutively expressed neo(r) mRNA derived from pSV2neo. The proteolytic digestion was found to be the most critical procedure. The in situ hybridization demonstrated that neo(r) mRNA was localized in the cytoplasm of the neo(r) cells. Cf2Th cells derived from a canine fetal thymus cell were transfected with the cloned provirus genome of a retrovirus produced in a human lymphoblastoid cell line, and transient expressions of viral RNA and antigens were monitored by in situ hybridization and immunoperoxidase staining, respectively. About 48 hours after transfection, several percent of the transfected cells were positive for expression of both viral RNA and antigens by these cytochemical methods. These observations indicate that in situ hybridization is a useful method for detecting the expression of the gene introduced into mammalian cells. en-copyright= kn-copyright= en-aut-name=MurakamiMasashi en-aut-sei=Murakami en-aut-mei=Masashi kn-aut-name=村上雅 kn-aut-sei=村上 kn-aut-mei=雅 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=in situハイブリダイゼイション kn-keyword=in situハイブリダイゼイション en-keyword=ビオチン標識プローブDNAトランスフェクション kn-keyword=ビオチン標識プローブDNAトランスフェクション en-keyword=遺伝子発現 kn-keyword=遺伝子発現 END start-ver=1.4 cd-journal=joma no-vol=552 cd-vols= no-issue=3 article-no= start-page=292 end-page=328 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200511 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Design and performance of the ABCD3TA ASIC for readout of silicon strip detectors in the ATLAS semiconductor tracke en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The ABCD3TA is a 128-channel ASIC with binary architecture for the readout of silicon strip particle detectors in the Semiconductor Tracker of the ATLAS experiment at the Large Hadron Collider (LHC). The chip comprises fast front-end and amplitude discriminator circuits using bipolar devices, a binary pipeline for first level trigger latency, a second level derandomising buffer and data compression circuitry based on CMOS devices. It has been designed and fabricated in a BiCMOS radiation resistant process. Extensive testing of the ABCD3TA chips assembled into detector modules show that the design meets the specifications and maintains the required performance after irradiation up to a total ionising dose of 10 Mrad and a 1-MeV neutron equivalent fluence of 2×1014 n/cm2, corresponding to 10 years of operation of the LHC at its design luminosity. Wafer screening and quality assurance procedures have been developed and implemented in large volume production to ensure that the chips assembled into modules meet the rigorous acceptance criteria.

en-copyright= kn-copyright= en-aut-name=CampabadalF en-aut-sei=Campabadal en-aut-mei=F kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FletaC en-aut-sei=Fleta en-aut-mei=C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KeyM en-aut-sei=Key en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=LozanoM en-aut-sei=Lozano en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MartinezC en-aut-sei=Martinez en-aut-mei=C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=PellegriniG en-aut-sei=Pellegrini en-aut-mei=G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=RafiJ M en-aut-sei=Rafi en-aut-mei=J M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UllanM en-aut-sei=Ullan en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=JohansenL G en-aut-sei=Johansen en-aut-mei=L G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=MohnB en-aut-sei=Mohn en-aut-mei=B kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=OyeO en-aut-sei=Oye en-aut-mei=O kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=SolbergA O en-aut-sei=Solberg en-aut-mei=A O kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=StuguB en-aut-sei=Stugu en-aut-mei=B kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=CiocioA en-aut-sei=Ciocio en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=ElyR en-aut-sei=Ely en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=FadeyevV en-aut-sei=Fadeyev en-aut-mei=V kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=GilchrieseM en-aut-sei=Gilchriese en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=HaberC en-aut-sei=Haber en-aut-mei=C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= en-aut-name=SiegristJ en-aut-sei=Siegrist en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=19 ORCID= en-aut-name=SpielerH en-aut-sei=Spieler en-aut-mei=H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=20 ORCID= en-aut-name=VuC en-aut-sei=Vu en-aut-mei=C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=21 ORCID= en-aut-name=BellP J en-aut-sei=Bell en-aut-mei=P J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=22 ORCID= en-aut-name=CharltonD G en-aut-sei=Charlton en-aut-mei=D G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=23 ORCID= en-aut-name=DowellJ D en-aut-sei=Dowell en-aut-mei=J D kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=24 ORCID= en-aut-name=GallopB J en-aut-sei=Gallop en-aut-mei=B J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=25 ORCID= en-aut-name=HomerR J en-aut-sei=Homer en-aut-mei=R J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=26 ORCID= en-aut-name=JovanovicP en-aut-sei=Jovanovic en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=27 ORCID= en-aut-name=MahoutG en-aut-sei=Mahout en-aut-mei=G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=28 ORCID= en-aut-name=McMahonT J en-aut-sei=McMahon en-aut-mei=T J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=29 ORCID= en-aut-name=WilsonJ A en-aut-sei=Wilson en-aut-mei=J A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=30 ORCID= en-aut-name=BarrA J en-aut-sei=Barr en-aut-mei=A J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=31 ORCID= en-aut-name=CarterJ R en-aut-sei=Carter en-aut-mei=J R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=32 ORCID= en-aut-name=GoodrickM J en-aut-sei=Goodrick en-aut-mei=M J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=33 ORCID= en-aut-name=HillJ C en-aut-sei=Hill en-aut-mei=J C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=34 ORCID= en-aut-name=LesterC G en-aut-sei=Lester en-aut-mei=C G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=35 ORCID= en-aut-name=ParkerM A en-aut-sei=Parker en-aut-mei=M A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=36 ORCID= en-aut-name=RobinsonD en-aut-sei=Robinson en-aut-mei=D kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=37 ORCID= en-aut-name=AnghinolfiF en-aut-sei=Anghinolfi en-aut-mei=F kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=38 ORCID= en-aut-name=ChesiE en-aut-sei=Chesi en-aut-mei=E kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=39 ORCID= en-aut-name=JarronP en-aut-sei=Jarron en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=40 ORCID= en-aut-name=KaplonJ en-aut-sei=Kaplon en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=41 ORCID= en-aut-name=MacphersonA en-aut-sei=Macpherson en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=42 ORCID= en-aut-name=PerneggerH en-aut-sei=Pernegger en-aut-mei=H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=43 ORCID= en-aut-name=PritchardT en-aut-sei=Pritchard en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=44 ORCID= en-aut-name=RoeS en-aut-sei=Roe en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=45 ORCID= en-aut-name=RudgeA en-aut-sei=Rudge en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=46 ORCID= en-aut-name=WeilhammerP en-aut-sei=Weilhammer en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=47 ORCID= en-aut-name=BialasW en-aut-sei=Bialas en-aut-mei=W kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=48 ORCID= en-aut-name=DabrowskiW en-aut-sei=Dabrowski en-aut-mei=W kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=49 ORCID= en-aut-name=DwuznikM en-aut-sei=Dwuznik en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=50 ORCID= en-aut-name=ToczekB en-aut-sei=Toczek en-aut-mei=B kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=51 ORCID= en-aut-name=KopernyS en-aut-sei=Koperny en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=52 ORCID= en-aut-name=BruckmanP en-aut-sei=Bruckman en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=53 ORCID= en-aut-name=GadomskiS en-aut-sei=Gadomski en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=54 ORCID= en-aut-name=GornickiE en-aut-sei=Gornicki en-aut-mei=E kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=55 ORCID= en-aut-name=MaleckiP en-aut-sei=Malecki en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=56 ORCID= en-aut-name=MoszczynskiA en-aut-sei=Moszczynski en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=57 ORCID= en-aut-name=StaneckaE en-aut-sei=Stanecka en-aut-mei=E kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=58 ORCID= en-aut-name=SzczygielR en-aut-sei=Szczygiel en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=59 ORCID= en-aut-name=TuralaM en-aut-sei=Turala en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=60 ORCID= en-aut-name=WolterW en-aut-sei=Wolter en-aut-mei=W kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=61 ORCID= en-aut-name=AndricekL en-aut-sei=Andricek en-aut-mei=L kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=62 ORCID= en-aut-name=BethkeS en-aut-sei=Bethke en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=63 ORCID= en-aut-name=HauffD en-aut-sei=Hauff en-aut-mei=D kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=64 ORCID= en-aut-name=KudlatyJ en-aut-sei=Kudlaty en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=65 ORCID= en-aut-name=LutzG en-aut-sei=Lutz en-aut-mei=G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=66 ORCID= en-aut-name=MoserH -G en-aut-sei=Moser en-aut-mei=H -G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=67 ORCID= en-aut-name=NisiusR en-aut-sei=Nisius en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=68 ORCID= en-aut-name=RichterR en-aut-sei=Richter en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=69 ORCID= en-aut-name=SchieckJ en-aut-sei=Schieck en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=70 ORCID= en-aut-name=ColijnA-P en-aut-sei=Colijn en-aut-mei=A-P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=71 ORCID= en-aut-name=CornelissenT en-aut-sei=Cornelissen en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=72 ORCID= en-aut-name=GorfineG W en-aut-sei=Gorfine en-aut-mei=G W kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=73 ORCID= en-aut-name=HartjesF G en-aut-sei=Hartjes en-aut-mei=F G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=74 ORCID= en-aut-name=HesseyN P en-aut-sei=Hessey en-aut-mei=N P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=75 ORCID= en-aut-name=JongP de en-aut-sei=Jong en-aut-mei=P de kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=76 ORCID= en-aut-name=KluitR en-aut-sei=Kluit en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=77 ORCID= en-aut-name=KoffemanE en-aut-sei=Koffeman en-aut-mei=E kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=78 ORCID= en-aut-name=MuijsA J. M en-aut-sei=Muijs en-aut-mei=A J. M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=79 ORCID= en-aut-name=PeetersS J. M en-aut-sei=Peeters en-aut-mei=S J. M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=80 ORCID= en-aut-name=EijkB van en-aut-sei=Eijk en-aut-mei=B van kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=81 ORCID= en-aut-name=NakanoI en-aut-sei=Nakano en-aut-mei=I kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=82 ORCID= en-aut-name=TanakaR en-aut-sei=Tanaka en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=83 ORCID= en-aut-name=DorholtO en-aut-sei=Dorholt en-aut-mei=O kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=84 ORCID= en-aut-name=DanielsenK M en-aut-sei=Danielsen en-aut-mei=K M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=85 ORCID= en-aut-name=HuseT en-aut-sei=Huse en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=86 ORCID= en-aut-name=SandakerH en-aut-sei=Sandaker en-aut-mei=H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=87 ORCID= en-aut-name=StapnesS en-aut-sei=Stapnes en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=88 ORCID= en-aut-name=KunduN en-aut-sei=Kundu en-aut-mei=N kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=89 ORCID= en-aut-name=NickersonR B en-aut-sei=Nickerson en-aut-mei=R B kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=90 ORCID= en-aut-name=WeidbergA en-aut-sei=Weidberg en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=91 ORCID= en-aut-name=BohmJ en-aut-sei=Bohm en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=92 ORCID= en-aut-name=MikestikovaM en-aut-sei=Mikestikova en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=93 ORCID= en-aut-name=StastnyJ en-aut-sei=Stastny en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=94 ORCID= en-aut-name=BroklovaZ en-aut-sei=Broklova en-aut-mei=Z kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=95 ORCID= en-aut-name=BrozJ en-aut-sei=Broz en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=96 ORCID= en-aut-name=DolezalZ en-aut-sei=Dolezal en-aut-mei=Z kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=97 ORCID= en-aut-name=KodysP en-aut-sei=Kodys en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=98 ORCID= en-aut-name=KubikP en-aut-sei=Kubik en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=99 ORCID= en-aut-name=ReznicekP en-aut-sei=Reznicek en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=100 ORCID= en-aut-name=VorobelV en-aut-sei=Vorobel en-aut-mei=V kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=101 ORCID= en-aut-name=WilhelmI en-aut-sei=Wilhelm en-aut-mei=I kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=102 ORCID= en-aut-name=CermakP en-aut-sei=Cermak en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=103 ORCID= en-aut-name=ChrenD en-aut-sei=Chren en-aut-mei=D kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=104 ORCID= en-aut-name=HorazdovskyT en-aut-sei=Horazdovsky en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=105 ORCID= en-aut-name=LinhartV en-aut-sei=Linhart en-aut-mei=V kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=106 ORCID= en-aut-name=PospisilS en-aut-sei=Pospisil en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=107 ORCID= en-aut-name=SinorM en-aut-sei=Sinor en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=108 ORCID= en-aut-name=SolarM en-aut-sei=Solar en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=109 ORCID= en-aut-name=SopkoB en-aut-sei=Sopko en-aut-mei=B kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=110 ORCID= en-aut-name=SteklI en-aut-sei=Stekl en-aut-mei=I kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=111 ORCID= en-aut-name=ApsimonR J en-aut-sei=Apsimon en-aut-mei=R J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=112 ORCID= en-aut-name=BatchelorL E en-aut-sei=Batchelor en-aut-mei=L E kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=113 ORCID= en-aut-name=BizzellJ P en-aut-sei=Bizzell en-aut-mei=J P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=114 ORCID= en-aut-name=FalconerN G en-aut-sei=Falconer en-aut-mei=N G kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=115 ORCID= en-aut-name=FrenchM J en-aut-sei=French en-aut-mei=M J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=116 ORCID= en-aut-name=GibsonM D en-aut-sei=Gibson en-aut-mei=M D kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=117 ORCID= en-aut-name=HaywoodS J en-aut-sei=Haywood en-aut-mei=S J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=118 ORCID= en-aut-name=MatsonR M en-aut-sei=Matson en-aut-mei=R M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=119 ORCID= en-aut-name=McMahonS J en-aut-sei=McMahon en-aut-mei=S J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=120 ORCID= en-aut-name=MorrisseyM en-aut-sei=Morrissey en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=121 ORCID= en-aut-name=MurrayW J en-aut-sei=Murray en-aut-mei=W J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=122 ORCID= en-aut-name=PhillipsP W en-aut-sei=Phillips 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aut-affil-num=131 ORCID= en-aut-name=KachiguineS en-aut-sei=Kachiguine en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=132 ORCID= en-aut-name=RosenbaumF en-aut-sei=Rosenbaum en-aut-mei=F kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=133 ORCID= en-aut-name=SadrozinskiH F. -W en-aut-sei=Sadrozinski en-aut-mei=H F. -W kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=134 ORCID= en-aut-name=SeidenA en-aut-sei=Seiden en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=135 ORCID= en-aut-name=SpencerE en-aut-sei=Spencer en-aut-mei=E kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=136 ORCID= en-aut-name=WilderM en-aut-sei=Wilder en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=137 ORCID= en-aut-name=AkimotoT en-aut-sei=Akimoto en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=138 ORCID= en-aut-name=HaraK en-aut-sei=Hara en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=139 ORCID= en-aut-name=TanizakiK 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kn-affil=Instituto de Microelectronica de Barcelona, IMB-CNM, CSIC, Barcelona, Spain affil-num=9 en-affil= kn-affil=University of Bergen, Bergen, Norway affil-num=10 en-affil= kn-affil=University of Bergen, Bergen, Norway affil-num=11 en-affil= kn-affil=University of Bergen, Bergen, Norway affil-num=12 en-affil= kn-affil=University of Bergen, Bergen, Norway affil-num=13 en-affil= kn-affil=University of Bergen, Bergen, Norway affil-num=14 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=15 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=16 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=17 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=18 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=19 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=20 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=21 en-affil= kn-affil=Lawrence Berkeley Laboratory and University of California, Berkeley, California, USA affil-num=22 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=23 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=24 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=25 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=26 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=27 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=28 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=29 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=30 en-affil= kn-affil=School of Physics and Astronomy, The University of Birmingham, Birmingham, UK affil-num=31 en-affil= kn-affil=Cavendish Laboratory, Cambridge University, Cambridge, UK affil-num=32 en-affil= kn-affil=Cavendish Laboratory, Cambridge University, Cambridge, UK affil-num=33 en-affil= kn-affil=Cavendish Laboratory, Cambridge University, Cambridge, UK affil-num=34 en-affil= kn-affil=Cavendish Laboratory, Cambridge University, Cambridge, UK affil-num=35 en-affil= kn-affil=Cavendish Laboratory, Cambridge University, Cambridge, UK affil-num=36 en-affil= kn-affil=Cavendish Laboratory, Cambridge University, Cambridge, UK affil-num=37 en-affil= kn-affil=Cavendish Laboratory, Cambridge University, Cambridge, UK affil-num=38 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=39 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=40 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=41 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=42 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=43 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=44 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=45 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=46 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=47 en-affil= kn-affil=CERN, European Organization for Nuclear Research, Geneva, Switzerland affil-num=48 en-affil= kn-affil=Faculty Physics and Applied Computer Science, AGH University Science Technology, Krakow, Poland affil-num=49 en-affil= kn-affil=Faculty Physics and Applied Computer Science, AGH University Science Technology, Krakow, Poland affil-num=50 en-affil= kn-affil=Faculty Physics and Applied Computer Science, AGH University Science Technology, Krakow, Poland affil-num=51 en-affil= kn-affil=Faculty Physics and Applied Computer Science, AGH University Science Technology, Krakow, Poland affil-num=52 en-affil= kn-affil=Faculty Physics and Applied Computer Science, AGH University Science Technology, Krakow, Poland affil-num=53 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=54 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=55 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=56 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=57 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=58 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=59 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=60 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=61 en-affil= kn-affil=The Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland affil-num=62 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=63 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=64 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=65 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=66 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=67 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=68 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=69 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=70 en-affil= kn-affil=Max-Planck-Institut fur Physik, Munchen, Germany affil-num=71 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=72 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=73 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=74 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=75 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=76 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=77 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=78 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=79 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=80 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=81 en-affil= kn-affil=NIKHEF, Amsterdam, The Netherlands affil-num=82 en-affil= kn-affil=Physics Department, Okayama University, Okayama, Japan affil-num=83 en-affil= kn-affil=Physics Department, Okayama University, Okayama, Japan affil-num=84 en-affil= kn-affil=University of Oslo, Oslo, Norway affil-num=85 en-affil= kn-affil=University of Oslo, Oslo, Norway affil-num=86 en-affil= kn-affil=University of Oslo, Oslo, Norway affil-num=87 en-affil= kn-affil=University of Oslo, Oslo, Norway affil-num=88 en-affil= kn-affil=University of Oslo, Oslo, Norway affil-num=89 en-affil= kn-affil=Department of Physics, Oxford University, Oxford, UK affil-num=90 en-affil= kn-affil=Department of Physics, Oxford University, Oxford, UK affil-num=91 en-affil= kn-affil=Department of Physics, Oxford University, Oxford, UK affil-num=92 en-affil= kn-affil=Academy of Sciences of the Czech Republic (ASCR), Prague, Czech Republic affil-num=93 en-affil= kn-affil=Academy of Sciences of the Czech Republic (ASCR), Prague, Czech Republic affil-num=94 en-affil= kn-affil=Academy of Sciences of the Czech Republic (ASCR), Prague, Czech Republic affil-num=95 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=96 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=97 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=98 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=99 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=100 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=101 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=102 en-affil= kn-affil=Charles University, Prague, Czech Republic affil-num=103 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=104 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=105 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=106 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=107 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=108 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=109 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=110 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=111 en-affil= kn-affil=Czech Technical University, Prague, Czech Republic affil-num=112 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=113 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=114 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=115 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=116 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=117 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=118 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=119 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=120 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=121 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=122 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=123 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=124 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=125 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=126 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=127 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=128 en-affil= kn-affil=Rutherford Appleton Laboratory, Chilton, Didcot, UK affil-num=129 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=130 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=131 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=132 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=133 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=134 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=135 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=136 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=137 en-affil= kn-affil=Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, California, USA affil-num=138 en-affil= kn-affil=Institute of Physics, University of Tsukuba, Tsukuba, Japan affil-num=139 en-affil= kn-affil=Institute of Physics, University of Tsukuba, Tsukuba, Japan affil-num=140 en-affil= kn-affil=Institute of Physics, University of Tsukuba, Tsukuba, Japan affil-num=141 en-affil= kn-affil=Department of Radiation Sciences, Uppsala Universit, Uppsala, Sweden affil-num=142 en-affil= kn-affil=Department of Radiation Sciences, Uppsala Universit, Uppsala, Sweden affil-num=143 en-affil= kn-affil=Department of Radiation Sciences, Uppsala Universit, Uppsala, Sweden affil-num=144 en-affil= kn-affil=Department of Radiation Sciences, Uppsala Universit, Uppsala, Sweden affil-num=145 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=146 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=147 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=148 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=149 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=150 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=151 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=152 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=153 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=154 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=155 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=156 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=157 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain affil-num=158 en-affil= kn-affil=Instituto de Fisica Corpuscular (IFIC), Universidad de Valencia-CSIC, Valencia, Spain en-keyword=Front-end electronics kn-keyword=Front-end electronics en-keyword=Binary readout kn-keyword=Binary readout en-keyword=Silicon strip detectors kn-keyword=Silicon strip detectors en-keyword=Tracking detectors kn-keyword=Tracking detectors en-keyword=Radiation damage kn-keyword=Radiation damage END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=3 article-no= start-page=252 end-page=256 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20049 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Assessment of MRI and dynamic contrast-enhanced MRI in the differential diagnosis of adenomatoid odontogenic tumor en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The radiographical differentiation of adenomatoid odontogenic tumor (AOT) from dentigerous cysts, calcifying odontogenic cysts, calcifying epithelial odontogenic tumors, odontogenic keratocysts, and amelobastomas is sometimes difficult. We attempted to differentiate AOT from other lesions similar to AOT in radiographic findings using MRI. The MRI features of AOT in our 3 cases included homogeneous low SI in the cystic portion and homogeneous intermediate SI in the solid portion on T1WI, homogeneous high SI in the cystic portion and intermediate to slightly high SI in the solid portion on T2WI, and enhancement of only the solid portion on CE-T1WI although non of the sequences included SI of calcifications. The contrast index curves in the 3 cases of AOT showed a gradual increase to 300 s, which signified a benign tumor. These MRI features were characteristic features of AOT and might be a basis for differentiating AOT from the above possible lesions in radiographic examinations.

en-copyright= kn-copyright= en-aut-name=AsaumiJun-ichi en-aut-sei=Asaumi en-aut-mei=Jun-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YanagiYoshinobu en-aut-sei=Yanagi en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KonouchiHironobu en-aut-sei=Konouchi en-aut-mei=Hironobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HisatomiMiki en-aut-sei=Hisatomi en-aut-mei=Miki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsuzakiHidenobu en-aut-sei=Matsuzaki en-aut-mei=Hidenobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShigeharaHiroshi en-aut-sei=Shigehara en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KishiKanji en-aut-sei=Kishi en-aut-mei=Kanji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=AOT kn-keyword=AOT en-keyword=odontogenic tumor kn-keyword=odontogenic tumor en-keyword=odontogenic cyst kn-keyword=odontogenic cyst en-keyword=MRI kn-keyword=MRI en-keyword=DCE-MRI kn-keyword=DCE-MRI END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200311 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Multiple roles of TNF super family members in corpus luteum function en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The main function of the corpus luteum (CL) is the production of progesterone. Adequate luteal progesterone is crucial for determining the physiological duration of the estrous cycle and for achieving a successful pregnancy. The CL is regulated not only by hypophyseal gonadotropin, but also by a number of cytokines that are locally produced. Tumor necrosis factor-α (TNF) and its specific receptors (TNFR) are present in the CL of many species. TNF plays multiple and likely important roles in CL function throughout the estrous cycle. TNF appears to have luteotropic and luteolytic roles in the CLs. In contrast, Fas ligand (Fas L), another member of TNF super family(TNF-SF), is primarily recognized for its apoptotic actions. Presumably, Fas L binds its cognate receptor (Fas) to induce structural luteolysis. This review is designed to focus on recent studies documenting the expression of TNF and Fas L, their receptors, and intracellular signaling mechanisms in the CL.

en-copyright= kn-copyright= en-aut-name=OkudaKiyoshi en-aut-sei=Okuda en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SakumotoRyosuke en-aut-sei=Sakumoto en-aut-mei=Ryosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=68 cd-vols= no-issue=11 article-no= start-page=1530 end-page=1536 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20076 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Serine racemases from barley, Hordeum vulgare L., and other plant species represent a distinct eukaryotic group: gene cloning and recombinant protein characterization en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Several D-amino acids have been identified in plants. However, the biosynthetic pathway to them is unclear. In this study, we cloned and sequenced a cDNA encoding a serine racemase from barley which contained an open reading frame encoding 337 amino acid residues. The deduced amino acid sequence showed significant identity to plant and mammalian serine racemases and contained conserved pyridoxal 5-phosphate (PLP)-binding lysine and PLP–interacting amino acid residues. The purified gene product catalyzed not only racemization of serine but also dehydration of serine to pyruvate. The enzyme requires PLP and divalent cations such as Ca2+, Mg2+, or Mn2+, but not ATP, whereas mammalian serine racemase activity is increased by ATP. In addition to the results regarding the effect of ATP on enzyme activity and the phylogenetic analysis of eukaryotic serine racemases, the antiserum against Arabidopsis serine racemase did not form a precipitate with barley and rice serine racemases. This suggests that plant serine racemases represent a distinct group in the eukaryotic serine racemase family and can be clustered into monocot and dicot types.

en-copyright= kn-copyright= en-aut-name=FujitaniYoshiyuki en-aut-sei=Fujitani en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HoriuchiTerumi en-aut-sei=Horiuchi en-aut-mei=Terumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ItoKazutoshi en-aut-sei=Ito en-aut-mei=Kazutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SugimotoManabu en-aut-sei=Sugimoto en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd. affil-num=4 en-affil= kn-affil=Okayama University en-keyword=Hordeum vulgare L. kn-keyword=Hordeum vulgare L. en-keyword=Oryza sativa kn-keyword=Oryza sativa en-keyword=Gramineae kn-keyword=Gramineae en-keyword=Pyridoxal 5-phosphate kn-keyword=Pyridoxal 5-phosphate en-keyword=Serine racemase kn-keyword=Serine racemase en-keyword=Serine dehydratase kn-keyword=Serine dehydratase en-keyword=d-Amino acid kn-keyword=d-Amino acid END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=15 article-no= start-page=5683 end-page=5686 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification of a vesicular nucleotide transporter en-subtitle= kn-subtitle= en-abstract= kn-abstract=

ATP is a major chemical transmitter in purinergic signal transmission. Before secretion, ATP is stored in secretory vesicles found in purinergic cells. Although the presence of active transport mechanisms for ATP has been postulated for a long time, the proteins responsible for its vesicular accumulation remains unknown. The transporter encoded by the human and mouse SLC17A9 gene, a novel member of an anion transporter family, was predominantly expressed in the brain and adrenal gland. The mouse and bovine counterparts were associated with adrenal chromaffin granules. Proteoliposomes containing purified transporter actively took up ATP, ADP, and GTP by using membrane potential as the driving force. The uptake properties of the reconstituted transporter were similar to that of the ATP uptake by synaptic vesicles and chromaffin granules. Suppression of endogenous SLC17A9 expression in PC12 cells decreased exocytosis of ATP. These findings strongly suggest that SLC17A9 protein is a vesicular nucleotide transporter and should lead to the elucidation of the molecular mechanism of ATP secretion in purinergic signal transmission.

en-copyright= kn-copyright= en-aut-name=SawadaKeisuke en-aut-sei=Sawada en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EchigoNoriko en-aut-sei=Echigo en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=JugeNarinobu en-aut-sei=Juge en-aut-mei=Narinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyajiTakaaki en-aut-sei=Miyaji en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OtsukaMasato en-aut-sei=Otsuka en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OmoteHiroshi en-aut-sei=Omote en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YamamotoAkitsugu en-aut-sei=Yamamoto en-aut-mei=Akitsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MoriyamaYoshinori en-aut-sei=Moriyama en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Nagahama Institute of Technology affil-num=8 en-affil= kn-affil=Okayama University en-keyword=chromaffin granule kn-keyword=chromaffin granule en-keyword=synaptic vesicle kn-keyword=synaptic vesicle en-keyword=ATP kn-keyword=ATP en-keyword=storage and exocytosis kn-keyword=storage and exocytosis en-keyword=purinergic signaling. kn-keyword=purinergic signaling. END start-ver=1.4 cd-journal=joma no-vol=366 cd-vols= no-issue=1 article-no= start-page=110 end-page=116 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Inhibition of tumor-stromal interaction through HGF/Met signaling by valproic acid en-subtitle= kn-subtitle= en-abstract= kn-abstract=Hepatocyte growth factor (HGF), which is produced by surrounding stromal cells, including fibroblasts and endothelial cells, has been shown to be a significant factor responsible for cancer cell invasion mediated by tumor-stromal interactions. We found in this study that the anti-tumor agent valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, strongly inhibited tumor-stromal interaction. VPA inhibited HGF production in fibroblasts induced by epidermal growth factor (EGF), platelet-derived growth factor, basic fibroblast growth factor, phorbol 12-myristate 13-acetate (PMA) and prostaglandin E-2 without any appreciable cytotoxic effect. Other HDAC inhibitors, including butyric acid and trichostatin A (TSA), showed similar inhibitory effects on HGF production stimulated by various inducers. Up-regulations of HGF gene expression induced by PMA and EGF were also suppressed by VPA and TSA. Furthermore, VPA significantly inhibited HGF-induced invasion of HepG2 hepatocellular carcinoma cells. VPA, however, did not affect the increases in phosphorylation of MAPK and Akt in HGF-treated HepG2 cells. These results demonstrated that VPA inhibited two critical processes of tumor-stromal interaction, induction of fibroblastic HGF production and HGF-induced invasion of HepG2 cells, and suggest that those activities serve for other anti-tumor mechanisms of VPA besides causing proliferation arrest, differentiation, and/or apoptosis of tumor cells. en-copyright= kn-copyright= en-aut-name=MatsumotoYohsuke en-aut-sei=Matsumoto en-aut-mei=Yohsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MotokiTakahiro en-aut-sei=Motoki en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KubotaSatoshi en-aut-sei=Kubota en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakigawaMasaharu en-aut-sei=Takigawa en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsubouchiHirohito en-aut-sei=Tsubouchi en-aut-mei=Hirohito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GohdaEiichi en-aut-sei=Gohda en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Department of Immunochemistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Immunochemistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Biochemistry and Molecular Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Biochemistry and Molecular Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Digestive Disease and Life-style Related Disease, Health Research Human and Environmental Sciences, Kagoshima University, Graduate School of Medicine and Dental Sciences affil-num=6 en-affil= kn-affil=Department of Immunochemistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=hepatocyte growth factor kn-keyword=hepatocyte growth factor en-keyword=valproic acid kn-keyword=valproic acid en-keyword=histone deacetylase inhibitor kn-keyword=histone deacetylase inhibitor en-keyword=butyric acid kn-keyword=butyric acid en-keyword=trichostatin A kn-keyword=trichostatin A en-keyword=induction kn-keyword=induction en-keyword=tumor invasion kn-keyword=tumor invasion en-keyword=dermal fibroblast kn-keyword=dermal fibroblast END start-ver=1.4 cd-journal=joma no-vol=272 cd-vols= no-issue=1 article-no= start-page=116 end-page=127 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20048 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Isolation of a transcriptionally active element of high copy number retrotransposons in sweetpotato genome en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Many plant retrotransposons have been characterized, but only three families (Tnt1, Tto1 and Tos17) have been demonstrated to be transpositionally competent. We followed a novel approach that enabled us to identify an active element of the Ty1-copia retrotransposon family with estimated 400 copies in the sweetpotato genome. DNA sequences of Ty1 -copia reverse transcriptase (RTase) from the sweetpotato genome were analyzed, and a group of retrotransposon copies probably formed by recent transposition events was further analyzed. 3’RACE on callus cDNA amplified transcripts containing long terminal repeats (LTR) of this group. The sequence -specific amplification polymorphism (S-SAP) patterns of the LTR sequence in the genomic DNA were compared between a normal plant and callus lines derived from it. A callus -specific S-SAP product was found into which the retrotransposon detected by the 3’RACE had been transposed apparently during cell culture. We conclude that our approach provides an effective way to identify active elements of retrotransposons with high copy numbers.

en-copyright= kn-copyright= en-aut-name=TaharaMakoto en-aut-sei=Tahara en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AokiTakahiro en-aut-sei=Aoki en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuzukaShinya en-aut-sei=Suzuka en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamashitaHiroki en-aut-sei=Yamashita en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TanakaMasaru en-aut-sei=Tanaka en-aut-mei=Masaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MatsunagaSachi en-aut-sei=Matsunaga en-aut-mei=Sachi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KokumaiShuhei en-aut-sei=Kokumai en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=JEOL Limited affil-num=3 en-affil= kn-affil=SK Foods Company, Limited affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=National Agricultural Research Center affil-num=6 en-affil= kn-affil=Kabaya Foods Corporation affil-num=7 en-affil= kn-affil=Okayama University en-keyword=retrotransposon kn-keyword=retrotransposon en-keyword=Ipomoea batatas kn-keyword=Ipomoea batatas en-keyword=S-SAP kn-keyword=S-SAP en-keyword=transposition kn-keyword=transposition END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=1-2 article-no= start-page=59 end-page=66 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200311 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Alternative methods to evaluate the protective ability of sunscreen against photo-genotoxicity en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Numerous epidemiological investigations show that sunlight is carcinogenic to humans and that the use of sunscreen may be effective in decreasing the risk of skin cancer. The biological activity of a sunscreen is evaluated by its ability to protect human skin from erythema as represented by a Sun Protection Factor (SPF). We propose that the sunscreen's protective effect against sunlight-induced genotoxicity, including mutation, should also be taken into account. In this study we examined the protective ability of sunscreens against natural sunlight and UV-induced genotoxicity in Drosophila somatic cells. We prepared three kinds of sunscreen samples, each with an SPF value of 20, 40 or 60 and compared their protective activities with commercial sunscreens. When a sunscreen of SPF 20, 40 or 60 was pasted on the plastic cover of a petri dish in which Drosophila larvae were exposed to the sun or UV lamps, genotoxicity decreased as the SPF of the sunscreen increased, relative to levels of genotoxicity observed in samples without sunscreen. However, the protective abilities of sunscreens were unexpectedly not so different from each other. To reveal the relationship between the protective activity of sunscreen and the wavelength of light with which larvae were irradiated through the sunscreen, we measured the transmittance of light through the petri dish cover on which the sunscreen was pasted. Effective protection was demonstrated by removing components of light whose wavelengths were below 315 nm. We suggest, that the measurement of anti-genotoxic activity and the determination of the wavelengths of light transmitted through the sunscreen should be an alternative method for evaluating the effectiveness of a sunscreen.

en-copyright= kn-copyright= en-aut-name=ToyoshimaMegumi en-aut-sei=Toyoshima en-aut-mei=Megumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HosodaKanako en-aut-sei=Hosoda en-aut-mei=Kanako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HanamuraMiho en-aut-sei=Hanamura en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkamotoKeinosuke en-aut-sei=Okamoto en-aut-mei=Keinosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KobayashiHiroshi en-aut-sei=Kobayashi en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NegishiTomoe en-aut-sei=Negishi en-aut-mei=Tomoe kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Shiseido Research Center affil-num=6 en-affil= kn-affil=Okayama University en-keyword=sunscreen kn-keyword=sunscreen en-keyword=sunlight kn-keyword=sunlight en-keyword=UVB kn-keyword=UVB en-keyword=somatic cell mutation kn-keyword=somatic cell mutation en-keyword=genotoxicity kn-keyword=genotoxicity en-keyword=transmittance kn-keyword=transmittance en-keyword=Drosophila melanogaster kn-keyword=Drosophila melanogaster END start-ver=1.4 cd-journal=joma no-vol=143 cd-vols= no-issue=4 article-no= start-page=467 end-page=473 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080626 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Selenite Assimilation into Formate Dehydrogenase H Depends on Thioredoxin Reductase in Escherichia coli en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Escherichia coli growing under anaerobic conditions produce H-2 and CO2 by the enzymatic cleavage of formate that is produced from pyruvate at the end of glycolysis. Selenium is an integral part of formate dehydrogenase H (FDHH), which catalyses the first step in the formate hydrogen lyase (FHL) system. The genes of FHL system are transcribed only under anaerobic conditions, in the presence of a sigma(54)-dependent transcriptional activator Fh1A that binds formate as an effector molecule. Although the formate addition to the nutrient media has been an established procedure for inducing high FDHH activity, we have identified a low-salt nutrient medium containing <0.1% NaCl enabled constitutive, high expression of FDHH even without formate and D-glucose added to the medium. The novel conditions allowed us to study the effects of disrupting genes like trxB (thioredoxin reductase) or gor (glutathione reductase) on the production of FDHH activity and also reductive assimilation of selenite (SeO32-) into the selenoprotein. Despite the widely accepted hypothesis that selenite is reduced by glutathione reductase-dependent system, it was demonstrated that trxB gene was essential for FDHH production and for labelling the FDHH polypeptide with Se-75-selenite. Our present study reports for the first time the physiological involvement of thioredoxin reductase in the reductive assimilation of selenite in E. coli.

en-copyright= kn-copyright= en-aut-name=TakahataMuneaki en-aut-sei=Takahata en-aut-mei=Muneaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AbeKatsumasa en-aut-sei=Abe en-aut-mei=Katsumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiharaHisaaki en-aut-sei=Mihara en-aut-mei=Hisaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KurokawaSuguru en-aut-sei=Kurokawa en-aut-mei=Suguru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YamamotoYoshihiro en-aut-sei=Yamamoto en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NakanoRyuhei en-aut-sei=Nakano en-aut-mei=Ryuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=EsakiNobuyoshi en-aut-sei=Esaki en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University affil-num=4 en-affil= kn-affil=Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University affil-num=5 en-affil= kn-affil=Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University affil-num=6 en-affil= kn-affil=Department of Genetics, Hyogo College of Medicine affil-num=7 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=8 en-affil= kn-affil=Department of Genetics, Hyogo College of Medicine affil-num=9 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University en-keyword=formate dehydrogenase H kn-keyword=formate dehydrogenase H en-keyword=selenite assimilation kn-keyword=selenite assimilation en-keyword=thioredoxin reductase kn-keyword=thioredoxin reductase END start-ver=1.4 cd-journal=joma no-vol=579 cd-vols= no-issue=19 article-no= start-page=4069 end-page=4075 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ZD1839 (Gefitinib, 'Iressa'), an epidermal growth factor receptor-tyrosine kinase inhibitor, enhances the anti-cancer effects of TRAIL in human esophageal squamous cell carcinoma en-subtitle= kn-subtitle= en-abstract= kn-abstract=The EGF (epidermal growth factor) receptor-tyrosine kinase inhibitor ZD1839 (Gefitinib, 'Iressa') blocks the cell signaling pathways involved in cell proliferation, survival, and angiogenesis in various cancer cells. TNF-related death apoptosis inducing ligand (TRAIL) acts as an anticancer agent. We investigated the antitumor effects of ZD1839 alone or in combination with TRAIL against human esophageal squamous cell cancer (ESCC) lines. Although all ESCC cells expressed EGF receptor at a protein level, the effect of ZD1839 on cell growth did not correlate with the level of EGFR expression and phosphorylation of EGF receptor protein in ESCC lines. ZD1839 caused a dose-dependent growth arrest at G0–G1 phase associated with increased p27 expression. As TE8 cells are resistant to TRAIL, we tested whether ZD1839 combined with TRAIL induced apoptosis of TE8 cells via the inhibition of EGF receptor signaling by ZD1839. ZD1839 inhibited the phosphorylation of Akt, and enhanced TRAIL-induced apoptosis via activation of caspase-3 and caspase-9, and inactivation of Bcl-xL. Our results indicated that ZD1839 has anti-cancer properties against human esophageal cancer cells. ZD1839 also augmented the anti-cancer activity of TRAIL, even in TRAIL-resistant tumors. These results suggest that treatment with ZD1839 and TRAIL may have potential in the treatment of ESCC patients. en-copyright= kn-copyright= en-aut-name=TeraishiFuminori en-aut-sei=Teraishi en-aut-mei=Fuminori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KagawaShunsuke en-aut-sei=Kagawa en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeTakanori en-aut-sei=Watanabe en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TangoYasuhisa en-aut-sei=Tango en-aut-mei=Yasuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawashimaTakeshi en-aut-sei=Kawashima en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=UmeokaTatsuo en-aut-sei=Umeoka en-aut-mei=Tatsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NisizakiMasahiko en-aut-sei=Nisizaki en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=2 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=3 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=4 en-affil= kn-affil=Department of Surgery, Shiga University of Medical Science affil-num=5 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=6 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=7 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=8 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry affil-num=9 en-affil= kn-affil=Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine and Dentistry en-keyword=epidermal growth factor receptor kn-keyword=epidermal growth factor receptor en-keyword=ZD1839 kn-keyword=ZD1839 en-keyword=akt kn-keyword=akt en-keyword=esophageal squamous cell cancer kn-keyword=esophageal squamous cell cancer en-keyword=tumor necrosis factor-related apoptosis inducing ligand kn-keyword=tumor necrosis factor-related apoptosis inducing ligand END start-ver=1.4 cd-journal=joma no-vol=27 cd-vols= no-issue=24 article-no= start-page=8739 end-page=8747 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080706 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-fl. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.

en-copyright= kn-copyright= en-aut-name=AgawaYasuo en-aut-sei=Agawa en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SarhanMoustafa en-aut-sei=Sarhan en-aut-mei=Moustafa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KageyamaYuji en-aut-sei=Kageyama en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkagiKazutaka en-aut-sei=Akagi en-aut-mei=Kazutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakaiMasayoshi en-aut-sei=Takai en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HashiyamaKazuya en-aut-sei=Hashiyama en-aut-mei=Kazuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=WadaTadashi en-aut-sei=Wada en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HandaHiroshi en-aut-sei=Handa en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IwamatsuAkihiro en-aut-sei=Iwamatsu en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=HiroseSusumu en-aut-sei=Hirose en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=UedaHitoshi en-aut-sei=Ueda en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=2 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=4 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University affil-num=5 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University affil-num=6 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=7 en-affil= kn-affil=Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology affil-num=8 en-affil= kn-affil=Integrated Research Institute, Tokyo Institute of Technology affil-num=9 en-affil= kn-affil=Protein Research Network, Inc. affil-num=10 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=11 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University en-keyword=SEQUENTIAL GENE ACTIVATION kn-keyword=SEQUENTIAL GENE ACTIVATION en-keyword=MATURATION PROTEIN-1 BLIMP-1 kn-keyword=MATURATION PROTEIN-1 BLIMP-1 en-keyword=STEROID-RECEPTOR SUPERFAMILY kn-keyword=STEROID-RECEPTOR SUPERFAMILY en-keyword=POLYTENE CHROMOSOMES kn-keyword=POLYTENE CHROMOSOMES en-keyword=SALIVARY-GLAND kn-keyword=SALIVARY-GLAND en-keyword=FUSHI-TARAZU kn-keyword=FUSHI-TARAZU en-keyword=EMBRYONIC-DEVELOPMENT kn-keyword=EMBRYONIC-DEVELOPMENT en-keyword=MOLECULAR-MECHANISM kn-keyword=MOLECULAR-MECHANISM en-keyword=TEMPORAL REGULATION kn-keyword=TEMPORAL REGULATION en-keyword=STAGE SPECIFICITY kn-keyword=STAGE SPECIFICITY END start-ver=1.4 cd-journal=joma no-vol=25 cd-vols= no-issue=18 article-no= start-page=4175 end-page=4186 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20060920 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Secretion of L-glutamate from osteoclasts through transcytosis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Osteoclasts are involved in the catabolism of the bone matrix and eliminate the resulting degradation products through transcytosis, but the molecular mechanism and regulation of transcytosis remain poorly understood. Upon differentiation, osteoclasts express vesicular glutamate transporter 1 (VGLUT1), which is essential for vesicular storage and subsequent exocytosis of glutamate in neurons. VGLUT1 is localized in transcytotic vesicles and accumulates L-glutamate. Osteoclasts secrete L-glutamate and the bone degradation products upon stimulation with KCl or ATP in a Ca2+-dependent manner. KCl- and ATP-dependent secretion of L-glutamate was absent in osteoclasts prepared from VGLUT1-/- knockout mice. Osteoclasts express mGluR8, a class III metabotropic glutamate receptor. Its stimulation by a specific agonist inhibits secretion of L-glutamate and bone degradation products, whereas its suppression by a specific antagonist stimulates bone resorption. Finally, it was found that VGLUT1-/- mice develop osteoporosis. Thus, in bone-resorbing osteoclasts, L-glutamate and bone degradation products are secreted through transcytosis and the released L-glutamate is involved in autoregulation of transcytosis. Glutamate signaling may play an important role in the bone homeostasis. en-copyright= kn-copyright= en-aut-name=MorimotoRiyo en-aut-sei=Morimoto en-aut-mei=Riyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UeharaShunsuke en-aut-sei=Uehara en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YatsushiroShouki en-aut-sei=Yatsushiro en-aut-mei=Shouki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=JugeNarinobu en-aut-sei=Juge en-aut-mei=Narinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HuaZhaolin en-aut-sei=Hua en-aut-mei=Zhaolin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SenohShigenori en-aut-sei=Senoh en-aut-mei=Shigenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=EchigoNoriko en-aut-sei=Echigo en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HayashiMitsuko en-aut-sei=Hayashi en-aut-mei=Mitsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MizoguchiToshihide en-aut-sei=Mizoguchi en-aut-mei=Toshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=NinomiyaTadashi en-aut-sei=Ninomiya en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=UdagawaNobuyuki en-aut-sei=Udagawa en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=OmoteHiroshi en-aut-sei=Omote en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=YamamotoAkitsugu en-aut-sei=Yamamoto en-aut-mei=Akitsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=EdwardsRobert H en-aut-sei=Edwards en-aut-mei=Robert H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=MoriyamaYoshinori en-aut-sei=Moriyama en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= affil-num=1 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Departments of Neurology and Physiology, Graduate Programs in Neuroscience and Cell Biology, University of California San Francisco School of Medicine affil-num=6 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Institute for Oral Science, Matsumoto Dental University affil-num=10 en-affil= kn-affil=Institute for Oral Science, Matsumoto Dental University affil-num=11 en-affil= kn-affil=Department of Biochemistry, Matsumoto Dental University affil-num=12 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=13 en-affil= kn-affil=Department of Cell Biology, Nagahama Institute of Bioscience and Technology affil-num=14 en-affil= kn-affil=Departments of Neurology and Physiology, Graduate Programs in Neuroscience and Cell Biology, University of California San Francisco School of Medicine affil-num=15 en-affil= kn-affil=Laboratory of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences en-keyword=osteoclast kn-keyword=osteoclast en-keyword=vesicular glutamate transporter kn-keyword=vesicular glutamate transporter en-keyword=transcytosis kn-keyword=transcytosis en-keyword=bone resorption kn-keyword=bone resorption END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=50 article-no= start-page=17923 end-page=17928 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A human transporter protein that mediates the final excretion step for toxic organic cations en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In mammals, toxic electrolytes of endogenous and exogenous origin are excreted through the urine and bile. Before excretion, these compounds cross numerous cellular membranes in a transporter-mediated manner. However, the protein transporters involved in the final excretion step are poorly understood. Here, we show that MATE1, a human and mouse orthologue of the multidrug and toxin extrusion (MATE) family conferring multidrug resistance on bacteria, is primarily expressed in the kidney and liver, where it is localized to the luminal membranes of the urinary tubules and bile canaliculi. When expressed in HEK293 cells, MATE1 mediates H+-coupled electroneutral exchange of tetraethylammonium (TEA) and 1-methyl-4-phenylpyridinium (MPP). Its substrate specificity is similar to those of renal and hepatic H+-coupled organic cations (OCs) export. Thus, MATE1 appears to be the long searched for polyspecific OC exporter that directly transports toxic OCs into urine and bile.

en-copyright= kn-copyright= en-aut-name=OtsukaMasato en-aut-sei=Otsuka en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsumotoTakuya en-aut-sei=Matsumoto en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MorimotoRiyo en-aut-sei=Morimoto en-aut-mei=Riyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AriokaShigeo en-aut-sei=Arioka en-aut-mei=Shigeo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OmoteHiroshi en-aut-sei=Omote en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriyamaYoshinori en-aut-sei=Moriyama en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=MATE kn-keyword=MATE en-keyword=multidrug export kn-keyword=multidrug export en-keyword=excretion kn-keyword=excretion en-keyword=toxin kn-keyword=toxin en-keyword=urinary tubule kn-keyword=urinary tubule en-keyword=bile canaliculus kn-keyword=bile canaliculus en-keyword=organic cation kn-keyword=organic cation en-keyword=H+/cation antiport. kn-keyword=H+/cation antiport. END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=3 article-no= start-page=134 end-page=139 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=200802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Association of elevated plasma B-type natriuretic peptide levels with paroxysmal atrial fibrillation in patients with nonobstructive hypertrophic cardiomyopathy en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Objectives: To investigate the relationship between the plasma B-type natriuretic peptide (BNP) level and the occurrence of atrial fibrillation (AF) in nonobstructive hypertrophic cardiomyopathy (HCM) patients.
Methods: Patients (n=97) were classified into chronic AF (CAF; n=14), paroxysmal AF (PAF; n=18) and normal sinus rhythm (NSR; n=65) groups. The plasma BNP values were analyzed with logarithmic transformation.
Results: The PAF group showed significantly higher plasma BNP levels than the NSR group [mean (range; -1 SD and +1 SD); 248.3 (143.5, 429.5) vs. 78.2 (27.9, 218.8 ng/L), p<0.0001]. The CAF group also showed significantly higher plasma BNP levels than the NSR group [291.1 (161.4, 524.8 ng/L), p<0.0001]. Multivariate analysis with other clinical factors selected association of PAF as one of the factors that increased the plasma BNP level.
Conclusions: The present study indicated that plasma BNP level is clinically useful for identification of nonobstructive HCM patients who have a risk of PAF.

en-copyright= kn-copyright= en-aut-name=MatsuuraHiroko en-aut-sei=Matsuura en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiTakashi en-aut-sei=Murakami en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HinaKazuyoshi en-aut-sei=Hina en-aut-mei=Kazuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoKeizo en-aut-sei=Yamamoto en-aut-mei=Keizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawamuraHiroshi en-aut-sei=Kawamura en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SogoTaiji en-aut-sei=Sogo en-aut-mei=Taiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShinohataRyoko en-aut-sei=Shinohata en-aut-mei=Ryoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UsuiShinichi en-aut-sei=Usui en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NinomiyaYoshifumi en-aut-sei=Ninomiya en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KusachiShozo en-aut-sei=Kusachi en-aut-mei=Shozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Department of Medicine and Medical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Cardiology, Cardiovascular Center, Sakakibara Hospital affil-num=3 en-affil= kn-affil=Department of Cardiology, Cardiovascular Center, Sakakibara Hospital affil-num=4 en-affil= kn-affil=Department of Cardiology, Cardiovascular Center, Sakakibara Hospital affil-num=5 en-affil= kn-affil=Department of Cardiology, Cardiovascular Center, Sakakibara Hospital affil-num=6 en-affil= kn-affil=Department of Cardiovascular Medicine, Takamatsu Red Cross Hospital affil-num=7 en-affil= kn-affil=Department of Medical Technology, Okayama University Graduate School of Health Sciences affil-num=8 en-affil= kn-affil=Department of Medical Technology, Okayama University Graduate School of Health Sciences affil-num=9 en-affil= kn-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=10 en-affil= kn-affil=Department of Medical Technology, Okayama University Graduate School of Health Sciences en-keyword=clinical study kn-keyword=clinical study en-keyword=cardiomyopathy kn-keyword=cardiomyopathy en-keyword=tachyarrhythmia kn-keyword=tachyarrhythmia en-keyword=enzyme immunoassay kn-keyword=enzyme immunoassay en-keyword=peptide kn-keyword=peptide en-keyword=sensitivity and specificity kn-keyword=sensitivity and specificity END start-ver=1.4 cd-journal=joma no-vol=131 cd-vols= no-issue=3 article-no= start-page=445 end-page=457 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200111 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Integral cohomology and chern classes of the special linear group over the ring of integers en-subtitle= kn-subtitle= en-abstract= kn-abstract=

This paper is devoted to the complete calculation of the additive structure of the 2-torsion of the integral cohomology of the innite special linear group SL(Z) over the ring of integers Z. This enables us to determine the best upper bound for the order of the Chern classes of all integral and rational representations of discrete groups.

en-copyright= kn-copyright= en-aut-name=ArlettazDominique en-aut-sei=Arlettaz en-aut-mei=Dominique kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AusoniChristian en-aut-sei=Ausoni en-aut-mei=Christian kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MimuraMamoru en-aut-sei=Mimura en-aut-mei=Mamoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YagitaNobuaki en-aut-sei=Yagita en-aut-mei=Nobuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Universite de Lausanne affil-num=2 en-affil= kn-affil=HG, ETH-Zentrum affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Ibaraki University END start-ver=1.4 cd-journal=joma no-vol=126 cd-vols= no-issue=3 article-no= start-page=427 end-page=429 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080622 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Expression of monocyte chemoattractant protein-1 in idiopathic dilated cardiomyopathy en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Immunological factors have been involved in the pathogenesis of dilated cardiomyopathy (DCM). The cytotoxic action of macrophages is one of the main factors causing cardiac myocyte damage. Monocyte chemoattractant protein-1 (MCP-1) is a major signal for the accumulation of monocytes/macrophages. We examined whether MCP-1 was expressed in the myocardium of DCM patients and whether the expression level was correlated with the degree of impairment of cardiac function. The expression of MCP-1 in the myocardium was determined by immunohistochemistry in endomyocardial biopsy samples from 13 patients. The expression of MCP-1 was found in all myocardial samples from DCM patients but not in those from control subjects. Positive staining for MCP-1 was distinct in cardiac myocytes, interstitium and infiltrating cells. Semi-quantitative analysis revealed that the expression of MCP-1 was inversely correlated with left ventricular ejection fraction. In conclusion, the expression level of MCP-1 in the myocardium was correlated with the degree of impairment of cardiac function in patients with DCM.

en-copyright= kn-copyright= en-aut-name=KobayashiMakoto en-aut-sei=Kobayashi en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamuraKazufumi en-aut-sei=Nakamura en-aut-mei=Kazufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KusanoKengo Fukushima en-aut-sei=Kusano en-aut-mei=Kengo Fukushima kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakamuraYoichi en-aut-sei=Nakamura en-aut-mei=Yoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=Ohta-OgoKeiko en-aut-sei=Ohta-Ogo en-aut-mei=Keiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NagaseSatoshi en-aut-sei=Nagase en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SakuragiSatoru en-aut-sei=Sakuragi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OheTohru en-aut-sei=Ohe en-aut-mei=Tohru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=aDepartment of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=aDepartment of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=cardiomyopathy kn-keyword=cardiomyopathy en-keyword=immunohistochemistry kn-keyword=immunohistochemistry en-keyword=monocyte kn-keyword=monocyte END start-ver=1.4 cd-journal=joma no-vol=66 cd-vols= no-issue=5 article-no= start-page=648 end-page=652 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=200805 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Radioactivity and radon emanation fraction of the granites sampled at Misasa and Badgastein en-subtitle= kn-subtitle= en-abstract= kn-abstract=The chemical composition was analyzed and the radioactivity, radon exhalation rate and emanation fraction were measured to investigate the characteristics of the granites sampled at Misasa and Badgastein, world famous for radon therapy. The Misasa granite was probably composed of quartz, albite and microcline. The Badgastein granite was probably composed of quartz and muscovite. The radon exhalation rates and emanation fractions of the Misasa granite were much higher than those of the Badgastein granite, regardless of the Ra-226 activity concentrations. en-copyright= kn-copyright= en-aut-name=SakodaAkihiro en-aut-sei=Sakoda en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HanamotoKatsumi en-aut-sei=Hanamoto en-aut-mei=Katsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IshimoriYu en-aut-sei=Ishimori en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagamatsuTomohiro en-aut-sei=Nagamatsu en-aut-mei=Tomohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamaokaKiyonori en-aut-sei=Yamaoka en-aut-mei=Kiyonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Health Sciences, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Health Sciences, Okayama University affil-num=3 en-affil= kn-affil=Ningyo-toge Environmental Engineering Center, Japan Atomic Energy Agency affil-num=4 en-affil= kn-affil=Graduate School of Health Sciences, Okayama University affil-num=5 en-affil= kn-affil=Graduate School of Health Sciences, Okayama University en-keyword=radioactivity kn-keyword=radioactivity en-keyword=radon emanation fraction kn-keyword=radon emanation fraction en-keyword=granite kn-keyword=granite en-keyword=Misasa kn-keyword=Misasa en-keyword=Badgastein kn-keyword=Badgastein END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=6 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Vortex structure in spinor f=2 bose-einstein condensates en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Extended Gross-Pitaevskii equations for the rotating F=2 condensate in a harmonic trap are solved both numerically and variationally using trial functions for each component of the wave function. Axially symmetric vortex solutions are analyzed and energies of polar and cyclic states are calculated. The equilibrium transitions between different phases with changing of the magnetization are studied. We show that at high magnetization the ground state of the system is determined by interaction in "density" channel, and at low magnetization spin interactions play a dominant role. Although there are five hyperfine states, all the particles are always condensed in one, two, or three states. Two interesting types of vortex structures are also discussed.

en-copyright= kn-copyright= en-aut-name=PogosovW V en-aut-sei=Pogosov en-aut-mei=W V kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawateR en-aut-sei=Kawate en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MizushimaT en-aut-sei=Mizushima en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MachidaK en-aut-sei=Machida en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=vortices kn-keyword=vortices END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20072 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Theory of tunneling spectroscopy in the larkin-ovchinnikov state en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We present a theory of tunneling spectroscopy for normal metal/Larkin-Ovchinnikov state junctions in which the spatial periodic modulation in the pair potential amplitude is taken into account. The tunneling spectra show the characteristic line shapes reflecting the minigap structures under the periodic pair potentials depending on the boundary condition of the pair potentials at the junction interface. These features are qualitatively different from the tunneling spectra of the Fulde-Ferrell state. We propose an experimental setup which identifies the superconducting state of CeCoIn5.

en-copyright= kn-copyright= en-aut-name=TanakaYukio en-aut-sei=Tanaka en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AsanoYasuhiro en-aut-sei=Asano en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IchikawaMasanori en-aut-sei=Ichikawa en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KashiwayaSatoshi en-aut-sei=Kashiwaya en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Nagoya University affil-num=2 en-affil= kn-affil=Japan Science and Technology Corporation affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=NeRI of National Institute of Advanced Industrial Science and Technology END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=1 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20061 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Sound velocity and multibranch bogoliubov spectrum of an elongated fermi superfluid in the BEC-BCS crossover en-subtitle= kn-subtitle= en-abstract= kn-abstract=We study properties of excited states of an elongated Fermi superfluid along the Bose-Einstein-condensate-BCS crossover including the unitarity limit. Analytic expressions for the sound velocity in an inhomogeneous as well as homogeneous Fermi superfluid along the crossover are obtained on the basis of the hydrodynamic theory. The complete excitation spectrum of axial quasiparticles with various discrete radial nodes is presented. We discuss the feasibility of measuring the sound velocity and the multibranch Bogoliubov spectrum experimentally. en-copyright= kn-copyright= en-aut-name=GhoshTarun Kanti en-aut-sei=Ghosh en-aut-mei=Tarun Kanti kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MachidaKazushige en-aut-sei=Machida en-aut-mei=Kazushige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=bose-einstein condensate kn-keyword=bose-einstein condensate en-keyword=gas kn-keyword=gas en-keyword=propagation kn-keyword=propagation en-keyword=resonance kn-keyword=resonance END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=5 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200511 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Multibranch Bogoliubov-Bloch spectrum of a cigar-shaped Bose condensate in an optical lattice en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We study properties of excited states of an array of weakly coupled quasi-two-dimensional Bose condensates by using the hydrodynamic theory. The spectrum of the axial excited states strongly depends on the coupling among the various discrete radial modes in a given symmetry. By including mode coupling within a given symmetry, the complete excitation spectrum of axial quasiparticles with various discrete radial nodes are presented. A single parameter which determines the strength of the mode coupling is identified. The excitation spectrum in the zero angular momentum sector can be observed by using the Bragg scattering experiments.

en-copyright= kn-copyright= en-aut-name=GhoshTarun Kanti en-aut-sei=Ghosh en-aut-mei=Tarun Kanti kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MachidaK. en-aut-sei=Machida en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=einstein kn-keyword=einstein en-keyword=condensate kn-keyword=condensate en-keyword=superfluid kn-keyword=superfluid en-keyword=arrays kn-keyword=arrays en-keyword=atoms kn-keyword=atoms en-keyword=wave kn-keyword=wave en-keyword=gas kn-keyword=gas END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20071 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Observation of a superconducting gap in boron-doped diamond by laser-excited photoemission spectroscopy en-subtitle= kn-subtitle= en-abstract= kn-abstract=

<p>We investigate the temperature (T)-dependent low-energy electronic structure of a boron-doped diamond thin film using ultrahigh resolution laser-excited photoemission spectroscopy. We observe a clear shift of the leading edge below T=11 K, indicative of a superconducting gap opening (Delta~0.78 meV at T=4.5 K). The gap feature is significantly broad and a well-defined quasiparticle peak is lacking even at the lowest temperature of measurement (=4.5 K). We discuss our results in terms of disorder effects on the normal state transport and superconductivity in this system.</p>

en-copyright= kn-copyright= en-aut-name=IshizakaK. en-aut-sei=Ishizaka en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EguchiR. en-aut-sei=Eguchi en-aut-mei=R. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsudaS. en-aut-sei=Tsuda en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YokotaT. en-aut-sei=Yokota en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ChainaniA. en-aut-sei=Chainani en-aut-mei=A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KissT. en-aut-sei=Kiss en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShimojimaT. en-aut-sei=Shimojima en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TogashiT. en-aut-sei=Togashi en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=WatanabeS. en-aut-sei=Watanabe en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ChenC. T. en-aut-sei=Chen en-aut-mei=C. T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=ZhangC. Q. en-aut-sei=Zhang en-aut-mei=C. Q. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TakanoY. en-aut-sei=Takano en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=NagaoM. en-aut-sei=Nagao en-aut-mei=M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=SakuguchiI. en-aut-sei=Sakuguchi en-aut-mei=I. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=TakenouchiT. en-aut-sei=Takenouchi en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=ShinS. en-aut-sei=Shin en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= affil-num=1 en-affil= kn-affil=University of Tokyo affil-num=2 en-affil= kn-affil=The Institute of Physical and Chemical Research (RIKEN) affil-num=3 en-affil= kn-affil=University of Tokyo affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=The Institute of Physical and Chemical Research (RIKEN) affil-num=6 en-affil= kn-affil=The Institute of Physical and Chemical Research (RIKEN) affil-num=7 en-affil= kn-affil=University of Tokyo affil-num=8 en-affil= kn-affil=The Institute of Physical and Chemical Research (RIKEN) affil-num=9 en-affil= kn-affil=University of Tokyo affil-num=10 en-affil= kn-affil=Chinese Academy of Science affil-num=11 en-affil= kn-affil=Chinese Academy of Science affil-num=12 en-affil= kn-affil=National Institute for Materials Science affil-num=13 en-affil= kn-affil=National Institute for Materials Science affil-num=14 en-affil= kn-affil=National Institute for Materials Science affil-num=15 en-affil= kn-affil=Waseda University affil-num=16 en-affil= kn-affil=University of Tokyo END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=2 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20068 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cyclic phase in f=2 spinor condensate:long-range order, kinks, and roughening transition en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We study the effect of thermal fluctuations on homogeneous infinite Bose-Einstein condensate with spin F=2 in the cyclic state, when atoms occupy three hyperfine states with m(F)=0,+/- 2. We use both the approach of small-amplitude oscillations and mapping of our model on the sine-Gordon model. We show that thermal fluctuations lead to the existence of the rough phase in one- and two-dimensional systems, when the presence of kinks is favorable. The structure and energy of a single kink are found. We also discuss the effect of thermal fluctuations on spin degrees of freedom in F=1 condensate.

en-copyright= kn-copyright= en-aut-name=PogosovW V en-aut-sei=Pogosov en-aut-mei=W V kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MachidaK en-aut-sei=Machida en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=bose-einstein condensate kn-keyword=bose-einstein condensate en-keyword=dynamics kn-keyword=dynamics en-keyword=gases kn-keyword=gases END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=2 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20068 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Thermal fluctuations of vortex clusters in quasi-two-dimensional bose-einstein condensates en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We study the thermal fluctuations of vortex positions in small vortex clusters in a harmonically trapped rotating Bose-Einstein condensate. It is shown that the order-disorder transition of two-shell clusters occurs via the decoupling of shells with respect to each other. The corresponding "melting" temperature depends strongly on the commensurability between numbers of vortices in shells. We show that melting can be achieved at experimentally attainable parameters and very low temperatures. Also studied is the effect of thermal fluctuations on vortices in an anisotropic trap with small quadrupole deformation. We show that thermal fluctuations lead to the decoupling of a vortex cluster from the pinning potential produced by this deformation. The decoupling temperatures are estimated and strong commensurability effects are revealed.

en-copyright= kn-copyright= en-aut-name=PogosovW V en-aut-sei=Pogosov en-aut-mei=W V kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MachidaK en-aut-sei=Machida en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=scissors mode kn-keyword=scissors mode en-keyword=gas kn-keyword=gas en-keyword=superfluidity kn-keyword=superfluidity en-keyword=transition kn-keyword=transition en-keyword=stability kn-keyword=stability en-keyword=phase kn-keyword=phase END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=8 article-no= start-page=1343 end-page=1349 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20068 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Using assessment of higher brain functions of children with GJB2-associated deafness and cochlear implants as a procedure to evaluate language development en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Objective: While investigators have reported that patients with GJB2-associated deafness and cochlear implants have preferable language development, the mechanisms of this phenomenon remains unknown. The goat of the present study was to assess higher brain functions of patients with GJB2-retated and GJB2-unrelated deafness as a method of evaluating language development.
Methods: Eight children with cochlear implants were subjected to genetic testing for GJB2 and underwent the Raven colored progressive matrices test, Rey's auditory verbal learning test, Rey's complex figure test, the standardized Language test for aphasia, the picture vocabulary test, and the standardized comprehension test for abstract words.
Results:Three children were diagnosed with GJB2-related deafness, and five children were diagnosed with GJB2-unrelated deafness. All three GJB2-related cases demonstrated normal range higher brain functions and fair language development. By contrast, one GJB2-unrelated case showed a semantic disorder, another demonstrated a visual cognitive disorder with dyslexia, and another had attention deficit-hyperactivity disorder.
Conclusions:Children with GJB2-unrelated deafness showed a high frequency of heterogeneous disorders that can affect proper language development. This difference between children with GJB2-retated and GJB2-unrelated deafness may account for the improved language development in children with GJB2-related deafness and cochlear implants. Further, genetic diagnosis of the non-syndromic hearing toss represents a useful tool for the preoperative prediction of outcomes following a cochlear implant procedure.

en-copyright= kn-copyright= en-aut-name=KawasakiAkihiro en-aut-sei=Kawasaki en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FukushimaKunihiro en-aut-sei=Fukushima en-aut-mei=Kunihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KataokaYuko en-aut-sei=Kataoka en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FukudaShoichiro en-aut-sei=Fukuda en-aut-mei=Shoichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishizakiKazunori en-aut-sei=Nishizaki en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=cochlear implant kn-keyword=cochlear implant en-keyword=higher brain function kn-keyword=higher brain function en-keyword=GJB2 kn-keyword=GJB2 en-keyword=language development kn-keyword=language development en-keyword=non-syndromic hearing loss kn-keyword=non-syndromic hearing loss en-keyword=learninng disability kn-keyword=learninng disability END start-ver=1.4 cd-journal=joma no-vol=115 cd-vols= no-issue=10 article-no= start-page=842 end-page=844 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200110 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=False-positive magnetic resonance image in the diagnosis of small acoustic neuroma en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A patient presented with sudden hearing loss on her Žrst visit to our department. Gadolinium-DTPA-enhanced magnetic resonance imaging (MRI) of the posterior cranial fossa portrayed an intracanalicular tumour image (2–3 mm), and the pure tone average (PTA) and speech discrimination score (SDS) values were 65 dB and 60 per cent, respectively. Surgical intervention to remove the suspected tumour was scheduled by the translabyrinthine approach. Intracanalicular observations by the retrolabyrinthine approach revealed limited oedema on the inferior vestibular nerve with vascular dilation. The tumour image disappeared two years after the operation. Surgical Žndings and the post-operative course advocate that gadolinium-DTPA-enriched MRI image of an intracanalicular lesion such as arachnoiditis might produce a false-positive result.

en-copyright= kn-copyright= en-aut-name=MaetaManabu en-aut-sei=Maeta en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SaitoRyusuke en-aut-sei=Saito en-aut-mei=Ryusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NamekiHideo en-aut-sei=Nameki en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama Saiseikai General Hospital affil-num=3 en-affil= kn-affil=Shizuoka Red Cross Hospital en-keyword=Acoustic Neuroma kn-keyword=Acoustic Neuroma en-keyword=Magnetic Resonance Imaging kn-keyword=Magnetic Resonance Imaging END start-ver=1.4 cd-journal=joma no-vol=118 cd-vols= no-issue=11 article-no= start-page=902 end-page=905 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=200411 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Abnormal direction of internal auditory canal and vestibulocochlear nerve en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Several internal auditory canal (IAC) anomalies have been reported.To our knowledge, only one case with anabnormal direction of the IAC has been reported in an infant with Pierre Robin syndrome. In this paper, wepresent the first report of two non-syndromic cases with abnormal IAC direction.

en-copyright= kn-copyright= en-aut-name=KariyaShin en-aut-sei=Kariya en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Nishizakikazunori en-aut-sei=Nishizaki en-aut-mei=kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=PaparellaMichael M. en-aut-sei=Paparella en-aut-mei=Michael M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=National Minami-Okayama Hospital affil-num=4 en-affil= kn-affil=Minnesota Ear Head and Neck Clinic en-keyword=Vestibulocochlear Nerve; Anatomy; Abnormalities; Nervous System Malformations kn-keyword=Vestibulocochlear Nerve; Anatomy; Abnormalities; Nervous System Malformations END start-ver=1.4 cd-journal=joma no-vol=117 cd-vols= no-issue=5 article-no= start-page=344 end-page=348 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20035 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Surgical intervention in middle-ear cholesterol granuloma en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Eleven patients who had been surgically treated from 1988 to 1999 were retrospectively reviewed in order to evaluate the efŽcacy of ventilation tube insertion and mastoidectomy with, or without, mastoid obliteration for intractable middle-ear cholesterol granuloma. The mean age registered was 17.2 years at the time of surgical treatment. All cases were unilaterally affected. Five ears were treated with simple mastoidectomy coupled with the insertion of a ventilation tube, while six others had additional mastoid obliteration. The hearing prognosis was excellent with an improved post-operative hearing level of 16.5.dB (cf. pre-operative 37.7.dB). However, morphological prognosis revealed two ears had a residual perforated tympanic membrane without otorrhoea after displacement of the ventilation tube. Of the remaining nine ears with intact placement of the ventilation tube, Žve had dry ears while four had occasional otorrhoea. Although the morphological prognosis was incomplete, treatments involving at least an insertion of a ventilation tube with thorough mastoidectomy were thought to be necessary.

en-copyright= kn-copyright= en-aut-name=MaetaManabu en-aut-sei=Maeta en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SaitoRyusuke en-aut-sei=Saito en-aut-mei=Ryusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakagawaFumio en-aut-sei=Nakagawa en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyaharaTakakazu en-aut-sei=Miyahara en-aut-mei=Takakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama Saiseikai General Hospital affil-num=3 en-affil= kn-affil=Okayama Saiseikai General Hospital affil-num=4 en-affil= kn-affil=Okayama Saiseikai General Hospital en-keyword=Granuloma kn-keyword=Granuloma en-keyword=Foreign-Body; Middle Ear Ventilation; Mastoid kn-keyword=Foreign-Body; Middle Ear Ventilation; Mastoid en-keyword=surgery kn-keyword=surgery END start-ver=1.4 cd-journal=joma no-vol=63 cd-vols= no-issue=1 article-no= start-page=o17 end-page=o20 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200701 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hydrogen bonding in two solid phases of phenazine-chloranilic acid (1/1) determined at 170 and 93 K en-subtitle= kn-subtitle= en-abstract= kn-abstract=The crystal structures in two solid phases, i.e. phase II stable between 146 and 253 K and phase IV below 136 K, of the title compound [phenazine-chloranilic acid (1/1), C12H8N2 center dot C6H2Cl2O4, in phase II, and phenazinium hydrogen chloranilate, C12H9N2+center dot C6HCl2O4-, in phase IV], have been determined. Both phases crystallize in P2(1), and each structure was refined as an inversion twin. In phase II, the phenazine and chloranilic acid molecules are arranged alternately through two kinds of O-H center dot center dot center dot N hydrogen bonds. In phase IV, salt formation occurs by donation of one H atom from the chloranilic acid molecule to the phenazine molecule; the resulting monocation and monoanion are linked by N-H center dot center dot center dot O and O-H center dot center dot center dot N hydrogen bonds. en-copyright= kn-copyright= en-aut-name=GotohKazuma en-aut-sei=Gotoh en-aut-mei=Kazuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AsajiTetsuo en-aut-sei=Asaji en-aut-mei=Tetsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IshidaHiroyuki en-aut-sei=Ishida en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Graduate School of Integrated Basic Sciences, College of Humanities and Sciences, Nihon University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=24 article-no= start-page=5800 end-page=5807 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080623 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=RhCl3/amine-catalyzed [2+2+2] cyclization of alkynes en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The RhCl3-3H(2)O/i-Pr2NEt-catalyzed [2+2+2] cyclotrimerization of alkynes has been achieved. The reaction can be widely used for various alkynes and provides tri- OF hexa-substituted benzenes regioselectively in high yields. The [2+2+2] cycloaddition of diynes and alkynes is also developed, and it affords benzene derivatives in moderate to high yields.

en-copyright= kn-copyright= en-aut-name=YoshidaKenta en-aut-sei=Yoshida en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MorimotoIchiro en-aut-sei=Morimoto en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MitsudoKoichi en-aut-sei=Mitsudo en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanakaHideo en-aut-sei=Tanaka en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University, affil-num=2 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University en-keyword=Rh/amine catalyst kn-keyword=Rh/amine catalyst en-keyword=cyclotrimerization kn-keyword=cyclotrimerization en-keyword=hexa-substituted benzene kn-keyword=hexa-substituted benzene END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue=24 article-no= start-page=4167 end-page=4179 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080710 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis and regioselective N- and O-alkylation of 1H- or 3H-[1,2,3]triazolo[4,5-d]pyrimidine-5,7(4H,6H)-diones (8-azaxanthines) and transformation of their 3-alkyl derivatives into 1-alkyl isomers en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Several alkylating agents, for example alkyl halides and dimethyl sulfate, were employed in aprotic Solvents Under a variety of conditions for the alkylation of mono- and disubstituted 1H- or 3H-[1,2,3]triazolo[4,5-d]pyrimidine-5,7(4H,6H)-diones, which were prepared by cyclization of the appropriate 5,6-diaminouracils with nitrous acid. The alkylation on the triazole ring in the presence of anhydrous potassium carbonate took place simultaneously at the 1- and 2-positions, with alkylation at the 2-position taking priority. Similar alkylation on the pyrimidine ring with an equivalent alkylating reagent took place only at the 4-position. The alkylation of 3,6-disubstituted derivatives at room temperature led to 5-O-alkylation accompanied by 4-N-alkylation, but at high temperature only 4-N-alkylation took place. Reaction of 3,4,6-trisubstituted derivatives with excess alkylating agent at high temperature leads to the formation of 1,4,6-trisubstituted derivatives with elimination of the 3-substituent.

en-copyright= kn-copyright= en-aut-name=IslamRafiqul en-aut-sei=Islam en-aut-mei=Rafiqul kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NagamatsuTomohisa en-aut-sei=Nagamatsu en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry, and Pharmaceutica­l Sciences, Okayama University affil-num=2 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry, and Pharmaceutica­l Sciences, Okayama University en-keyword=8-azaxanthines kn-keyword=8-azaxanthines en-keyword=triazolopyrimidines kn-keyword=triazolopyrimidines en-keyword=regioselectivity kn-keyword=regioselectivity en-keyword=alkylations kn-keyword=alkylations en-keyword=heterocycles kn-keyword=heterocycles END start-ver=1.4 cd-journal=joma no-vol=45 cd-vols= no-issue=11 article-no= start-page=2327 end-page=2329 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20043 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Enantioselective construction of biaryl part in the synthesis of stegane related compounds en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A Pd-mediated intramolecular aryl-aryl coupling reaction of phenyl benzoate derivatives were examined to form benzo[c]chromen-6-ones, and then enantioselective lactone-opening reaction with a borane-oxazaborolidine combination was carried out. The resulting biphenyl was transformed into a key intermediate for the stegane related compounds. The absolute configuration of the biphenyl is also discussed. Stegane and related compounds are important because of their interesting biological activities such as antileukemic properties.1 One of the most outstanding features of their chemical structures is an unsymmetrical 2,2’-disubstituted biphenyl moiety with an axial chirality (Figure 1). For the formation of such a biphenyl part in the syntheses of the stegane families, several approaches have been attempted such as photocyclization,2 Suzuki coupling,3 oxidative biaryl coupling,4 the SNAr reaction,5 Ullmann coupling,6 and the [2+2+2] three-component cyclization reaction.7

en-copyright= kn-copyright= en-aut-name=AbeHitoshi en-aut-sei=Abe en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakedaShigemitsu en-aut-sei=Takeda en-aut-mei=Shigemitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujitaTakuro en-aut-sei=Fujita en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishiokaKeisuke en-aut-sei=Nishioka en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakeuchiYasuo en-aut-sei=Takeuchi en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HarayamaTakashi en-aut-sei=Harayama en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=steganone kn-keyword=steganone en-keyword=palladium kn-keyword=palladium en-keyword=phenyl benzoate kn-keyword=phenyl benzoate en-keyword=lactone concept kn-keyword=lactone concept en-keyword=biary kn-keyword=biary END start-ver=1.4 cd-journal=joma no-vol=16 cd-vols= no-issue=2 article-no= start-page=922 end-page=940 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080115 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor studies. Part 4: Design, synthesis, antitumor activity, and molecular docking study of novel 2-substituted 2-deoxoflavin-5-oxides, 2-deoxoalloxazine-5-oxides, and their 5-deaza analogs en-subtitle= kn-subtitle= en-abstract= kn-abstract=Various novel 10-alkyl-2-deoxo-2-methylthioflavin-5-oxides and their 2-alkylamino derivatives were prepared by facile nitrosative cyclization of 6-(N-alkylanilino)-2-methylthiopyrimidin-4(3H)-ones followed by nucleophilic replacement of the 2-methylthio moiety by different amines, and acidic hydrolysis of the 2-methylthio moiety afforded the corresponding flavin derivatives. 2-Deoxo-2-methylthio-5-deazaalloxazines and 2-deoxo-2-methylthioalloxazine-5-oxides were also prepared by Vilsmeier reaction and by nitrosation of 6-anilino-2-methylthiopyrimidin-4(3H)-ones, respectively. Then, they were subjected to nucleophilic replacement with appropriate amines to produce the corresponding 2-alkylamino derivatives. Regiospecific N-3-alkylation of 2-deoxo-2-methylthioalloxazine-5-oxides was carried out with various alkylating agents in the usual way, The antitumor activities against CCRF-HSB-2 and KB tumor cells have been investigated in vitro, and many compounds showed promising antitumor activities. Furthermore, AutoDock molecular docking into PTK (PDB: 1t46) has been done for lead optimization of the aforementioned compounds as potential PTK inhibitors. en-copyright= kn-copyright= en-aut-name=AliHamed I. en-aut-sei=Ali en-aut-mei=Hamed I. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AshidaNoriyuki en-aut-sei=Ashida en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagamatsuTomohisa en-aut-sei=Nagamatsu en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=3 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=antitumor activity kn-keyword=antitumor activity en-keyword=flavin-5-oxide kn-keyword=flavin-5-oxide en-keyword=alloxazine-5-oxide kn-keyword=alloxazine-5-oxide en-keyword=AutoDock kn-keyword=AutoDock END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue= article-no= start-page=3643 end-page=3647 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080619 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A cross-metathesis approach to the stereocontrolled synthesis of the AB ring segment of ciguatoxin en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Synthesis of the AB ring segments of ciguatoxin is described. The present synthesis includes a Lewis acid mediated cyclization of allylstannane with aldehyde, cross-metathesis reaction introducing the side chain, and Grieco-Nishizawa dehydration on the A ring.

en-copyright= kn-copyright= en-aut-name=KadotaIsao en-aut-sei=Kadota en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AbeTakashi en-aut-sei=Abe en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UniMiyuki en-aut-sei=Uni en-aut-mei=Miyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakamuraHiroyoshi en-aut-sei=Takamura en-aut-mei=Hiroyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamamotoYoshinori en-aut-sei=Yamamoto en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Graduate School of Science, Tohoku University affil-num=3 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Department of Chemistry, Graduate School of Science, Tohoku University en-keyword=POLYCYCLIC ETHERS kn-keyword=POLYCYCLIC ETHERS en-keyword=ABSOLUTE-CONFIGURATION kn-keyword=ABSOLUTE-CONFIGURATION en-keyword=HYDROXY GROUP kn-keyword=HYDROXY GROUP en-keyword=MOIETY kn-keyword=MOIETY en-keyword=FRAGMENT kn-keyword=FRAGMENT en-keyword=SYSTEM kn-keyword=SYSTEM en-keyword=ROUTE kn-keyword=ROUTE en-keyword=CTX3C kn-keyword=CTX3C END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=51 article-no= start-page=11969 end-page=11975 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=200412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Reactivity of TEMPO anion as a nucleophile and its applications for selective transformations of haloalkanes or acyl halides to aldehydes en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Sodium 2,2,6,6-tetramethylpiperidine-N-oxide (TEMPO−Na+), generated by reduction of TEMPO· with sodium naphthalenide in THF, reacted with alkyl halides or acyl halides to produce O-alkylated or acylated TEMPOs, which were in turn oxidized with mCPBA or reduced with DIBAL-H to afford the corresponding aldehydes, thus accomplishing a new protocol for the halides-carbonyls conversion.

en-copyright= kn-copyright= en-aut-name=InokuchiTsutomu en-aut-sei=Inokuchi en-aut-mei=Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawafuchiHiroyuki en-aut-sei=Kawafuchi en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Toyama National College of Technology en-keyword=TEMPO and compounds kn-keyword=TEMPO and compounds en-keyword=Oxidation kn-keyword=Oxidation en-keyword=Reduction kn-keyword=Reduction en-keyword=mCPBA kn-keyword=mCPBA en-keyword=DIBAL-H kn-keyword=DIBAL-H en-keyword=Aldehyde kn-keyword=Aldehyde END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=15 article-no= start-page=2363 end-page=2365 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Facile synthetic procedure for and electrochemical properties of hexa(2-thienyl)benzenes directed toward electroactive materials en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In the presence of RhCl3 center dot 3H(2)O and i-Pr2NEt, the cyclotrimerization of di(2-thienyl)acetylenes proceeded smoothly to afford hexa(2-thienyl)benzenes. CV analysis of the hexa(2-thienyl)benzenes showed that they may be useful as electroactive materials.

en-copyright= kn-copyright= en-aut-name=YoshidaKenta en-aut-sei=Yoshida en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MorimotoIchiro en-aut-sei=Morimoto en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MitsudoKoichi en-aut-sei=Mitsudo en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanakaHideo en-aut-sei=Tanaka en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University en-keyword=Rh/amine catalyst kn-keyword=Rh/amine catalyst en-keyword=cyclotrimerization kn-keyword=cyclotrimerization en-keyword=hexa(2-thienyl)benzene kn-keyword=hexa(2-thienyl)benzene en-keyword=electroactive material kn-keyword=electroactive material END start-ver=1.4 cd-journal=joma no-vol=46 cd-vols= no-issue=18 article-no= start-page=3197 end-page=3200 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20055 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis of graphislactones A-D through a palladium-mediated biaryl coupling en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The chemical synthesis of graphislactones A-D was achieved through the Pd-mediated intramolecular biaryl coupling reaction of phenyl benzoate derivatives.

en-copyright= kn-copyright= en-aut-name=AbeHitoshi en-aut-sei=Abe en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishiokaKeisuke en-aut-sei=Nishioka en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakedaShigemitsu en-aut-sei=Takeda en-aut-mei=Shigemitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AraiMasatsugu en-aut-sei=Arai en-aut-mei=Masatsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakeuchiYasuo en-aut-sei=Takeuchi en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HarayamaTakashi en-aut-sei=Harayama en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=Palladium kn-keyword=Palladium en-keyword=Phenyl benzoate kn-keyword=Phenyl benzoate en-keyword=Graphislactone kn-keyword=Graphislactone en-keyword=Biaryl coupling kn-keyword=Biaryl coupling END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue=1 article-no= start-page=242 end-page=256 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor studies. Part 1: Design, synthesis, antitumor activity, and AutoDock study of 2-deoxo-2-phenyl-5-deazaflavins and 2-deoxo-2-phenylflavin-5-oxides as a new class of antitumor agents en-subtitle= kn-subtitle= en-abstract= kn-abstract=Novel 2-deoxo-2-phenyl-5-deazaflavins and 2-deoxo-2-phenylflavin-5-oxides were prepared as a new class of antitumor agents and showed significant antitumor activities against NCI-H 460, HCT 116, A 431, CCRF-HSB-2, and KB cell lines. In vivo investigation, 2-deoxo-10-methyl-2-phenyl-5-deazaflavin exhibited the effective antitumor activity against A 431 human adenocarcinoma cells transplanted subcutaneously into nude mouse. Furthermore, AutoDock study has been done by binding of the flavin analogs into PTK pp60(c-src), where a good correlation between their IC50 and AutoDock binding free energy was exhibited. In particular, 2-deoxo-2-phenylflavin-5-oxides exhibited the highest potential binding affinity within the binding pocket of PTK. en-copyright= kn-copyright= en-aut-name=AliHamed I.. en-aut-sei=Ali en-aut-mei=Hamed I.. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TomitaKeiichiro en-aut-sei=Tomita en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkahoEiichi en-aut-sei=Akaho en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KambaraHiroto en-aut-sei=Kambara en-aut-mei=Hiroto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiuraShinji en-aut-sei=Miura en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HayakawaHiroyuki en-aut-sei=Hayakawa en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AshidaNoriyuki en-aut-sei=Ashida en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KawashimaYutaka en-aut-sei=Kawashima en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamagishiTakehiro en-aut-sei=Yamagishi en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IkeyaHisao en-aut-sei=Ikeya en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=YonedaFumio en-aut-sei=Yoneda en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=NagamatsuTomohisa en-aut-sei=Nagamatsu en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil= kn-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Faculty of Pharmaceutical Sciences, High Technology Research Center (Life Science Center), and Center for Area Research and Development, Kobe Gakuin University affil-num=4 en-affil= kn-affil=Faculty of Pharmaceutical Sciences, High Technology Research Center (Life Science Center), and Center for Area Research and Development, Kobe Gakuin University affil-num=5 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=6 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=7 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=8 en-affil= kn-affil=Medicinal Research Laboratories, Taisho Pharmaceutical Co. affil-num=9 en-affil= kn-affil=Medicinal Research Laboratories, Taisho Pharmaceutical Co. affil-num=10 en-affil= kn-affil=Medicinal Research Laboratories, Taisho Pharmaceutical Co. affil-num=11 en-affil= kn-affil=Faculty of Pharmaceutical Sciences, Kyoto University affil-num=12 en-affil= kn-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=antitumor activity kn-keyword=antitumor activity en-keyword=Flavin analog kn-keyword=Flavin analog en-keyword=AutoDock kn-keyword=AutoDock en-keyword=protein tyrosine kinase kn-keyword=protein tyrosine kinase END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue= article-no= start-page=595 end-page=605 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=New dimeric flavans from gambir, an extract of Uncaria gambir en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Three new dimeric flavans, catechin-(4 alpha -> 8)-ent-epicatechin (7), gambirflavan D1 (8), and gambirflavan D2 (9), were isolated from gambir (an extract from the leaves and young twigs of Uncaria gambir), and their structures were determined based on spectroscopic and chemical data.

en-copyright= kn-copyright= en-aut-name=TaniguchiShoko en-aut-sei=Taniguchi en-aut-mei=Shoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KurodaKayo en-aut-sei=Kuroda en-aut-mei=Kayo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YoshikadoNaomi en-aut-sei=Yoshikado en-aut-mei=Naomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DoiKou-ichi en-aut-sei=Doi en-aut-mei=Kou-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TanabeMasahiro en-aut-sei=Tanabe en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShibataTakashi en-aut-sei=Shibata en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YoshidaTakashi en-aut-sei=Yoshida en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HatanoTsutomu en-aut-sei=Hatano en-aut-mei=Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University affil-num=2 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University affil-num=3 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University affil-num=4 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University affil-num=5 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University affil-num=6 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University affil-num=7 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University affil-num=8 en-affil= kn-affil=Faculty of Pharmaceutical Science, Okayama University en-keyword=PROCYANIDINS kn-keyword=PROCYANIDINS END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue=19 article-no= start-page=6336 end-page=6352 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20071001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor studies. Part 3: Design, synthesis, antitumor activity, and molecular docking study of novel 2-methylthio-, 2-amino-, and 2-(N-substituted amino)-10-alkyl-2-deoxo-5-deazaflavins en-subtitle= kn-subtitle= en-abstract= kn-abstract=Various novel 10-alkyl-2-deoxo-2-methylthio-5-deazaflavins have been synthesized by reaction of 6-(N-alkylanilino)-2-methylthiopyrimidin-4(3H)-ones with Vilsmeier reagent. The similar 2-(N-substituted amino) derivatives were prepared by nucleophilic replacement reaction of the 2-methylthio moiety by appropriate amines. The 2-oxo derivatives (i.e., 5-deazaflavins) were obtained by acidic hydrolysis of the 2-methylthio derivatives. The antitumor activities against CCRF-HSB-2 and KB cells and the antiviral activities against HSV-1 and HSV-2 have been investigated in vitro, and many compounds showed promising antitumor activities. Furthermore, AutoDock molecular docking into PTK has been done for lead optimization of these compounds as potential PTK inhibitors. Whereas, the designed 2-deoxo-5-deazaflavins connected with amino acids at the 2-position exhibited the good binding affinities into PTK with more hydrogen bonds. en-copyright= kn-copyright= en-aut-name=AliHamed I. en-aut-sei=Ali en-aut-mei=Hamed I. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AshidaNoriyuki en-aut-sei=Ashida en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagamatsuTomohisa en-aut-sei=Nagamatsu en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=3 en-affil= kn-affil=aDepartment of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=antitumor activity kn-keyword=antitumor activity en-keyword=5-deazaflavin kn-keyword=5-deazaflavin en-keyword=AutoDock kn-keyword=AutoDock en-keyword=protein tyrosine kn-keyword=protein tyrosine en-keyword=kinase kn-keyword=kinase END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=6 article-no= start-page=1021 end-page=1025 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Alkaline hydrothermal treatment of brominated high impact polystyrene (HIPS-Br) for bromine and bromine-free plastic recovery en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A method to recover both Br and Br-free plastic from brominated flame retardant high impact polystyrene (HIPS-Br) was proposed. HIPS-Br containing 15% Br was treated in autoclave at 280℃ using water or KOH solution of various amounts and concentrations. Hydrothermal treatment (30 ml water) leads to 90% debromination of 1 g HIPS-Br but plastic is strongly degraded and could not be recovered. previous termAlkalinenext term hydrothermal treatment (45 ml or 60 ml KOH 1 M) showed similar debromination for up to 12 g HIPS-Br and plastic was recovered as pellets with molecular weight distribution close to that of the initial material. Debromination occurs at melt plastic/KOH solution interface when liquid/vapour equilibrium is attained inside autoclave (280℃ and 7 MPa in our experimental conditions) and depends on the plastic amount/KOH volume ratio. The antimony oxide synergist from HIPS-Br remains in recovered plastic during treatment. A pictorial imagination of the proposed debromination process is presented.

en-copyright= kn-copyright= en-aut-name=BrebuMihai en-aut-sei=Brebu en-aut-mei=Mihai kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BhaskarThallada en-aut-sei=Bhaskar en-aut-mei=Thallada kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MutoAkinori en-aut-sei=Muto en-aut-mei=Akinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakataYusaku en-aut-sei=Sakata en-aut-mei=Yusaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Petru Poni Institute of Macromolecular Chemistry affil-num=2 en-affil= kn-affil=Department of Applied Chemistry, Faculty of Engineering, Okayama University affil-num=3 en-affil= kn-affil=Department of Applied Chemistry, Faculty of Engineering, Okayama University affil-num=4 en-affil= kn-affil=Department of Applied Chemistry, Faculty of Engineering, Okayama University en-keyword=Debromination kn-keyword=Debromination en-keyword=Alkaline hydrotreatment kn-keyword=Alkaline hydrotreatment en-keyword=HIPS-Br kn-keyword=HIPS-Br en-keyword=Bromine recovery kn-keyword=Bromine recovery END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=20020402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L. f. cv. Golden Leaves en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Background: The tropical plant Ficus microcarpa L. f. cv. Golden Leaves (GL) is a high-light sensitive tropical fig tree in which sun-leaves are yellow and shade-leaves are green. We compared the response of photosynthetic activities to strong light between GL and its wild-type (WT, Ficus microcarpa L. f.).

Results: Field measurements of maximum photosystem II (PSII) efficiency (Fv/Fm) of intact sunleaves in GL showed that photo synthetic activity was severely photoinhibited during the daytime (Fv/Fm = 0.46) and subsequently recovered in the evening (Fv/Fm = 0.76). In contrast, WT did not show any substantial changes of Fv/Fm values throughout the day (between 0.82 and 0.78). Light dependency of the CO2 assimilation rate in detached shade-leaves of GL showed a response similar to that in WT, suggesting no substantial difference in photosynthetic performance between them.Several indicators of photoinhibition, including declines in PSII reaction center protein (D1)content, Fv/Fm value, and O2 evolution and CO2 assimilation rates, all indicated that GL is much more susceptible to photoinhibition than WT. Kinetics of PAM chlorophyll a fluorescence revealed that nonphotochemical quenching (NPQ) capacity of GL was lower than that of WT.

Conclusion: We conclude that the photosynthetic apparatus of GL is more highly susceptible to photoinhibition than that of WT.

en-copyright= kn-copyright= en-aut-name=TakahashiShunichi en-aut-sei=Takahashi en-aut-mei=Shunichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TamashiroAyumu en-aut-sei=Tamashiro en-aut-mei=Ayumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SakihamaYasuko en-aut-sei=Sakihama en-aut-mei=Yasuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoYasusi en-aut-sei=Yamamoto en-aut-mei=Yasusi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawamitsuYoshinobu en-aut-sei=Kawamitsu en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YamasakiHideo en-aut-sei=Yamasaki en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus affil-num=2 en-affil= kn-affil=Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus affil-num=3 en-affil= kn-affil=Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus affil-num=4 en-affil= kn-affil=Department of Biology, Faculty of Science, Okayama University affil-num=5 en-affil= kn-affil=Laboratory of Crop Science, Faculty of Agriculture, University of the Ryukyus affil-num=6 en-affil= kn-affil=Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus en-keyword=Carbon dioxide kn-keyword=Carbon dioxide en-keyword=Chlorophyll kn-keyword=Chlorophyll en-keyword=Ficus kn-keyword=Ficus en-keyword=Genotype kn-keyword=Genotype en-keyword=Oxygen kn-keyword=Oxygen en-keyword=Photosynthesis kn-keyword=Photosynthesis en-keyword=Photosystem II protein complex kn-keyword=Photosystem II protein complex en-keyword=Plant leaves kn-keyword=Plant leaves END start-ver=1.4 cd-journal=joma no-vol=149 cd-vols= no-issue=6 article-no= start-page=2816 end-page=2825 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080625 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Aldosterone breakthrough caused by chronic blockage of angiotensin II type 1 receptors in human adrenocortical cells: Possible involvement of bone morphogenetic protein-6 actions en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Circulating aldosterone concentrations occasionally increase after initial suppression with angiotensin II (Ang II) converting enzyme inhibitors or Ang II type 1 receptor blockers (ARBs), a phenomenon referred to as aldosterone breakthrough. However, the underlying mechanism causing the aldosterone breakthrough remains unknown. Here we investigated whether aldosterone breakthrough occurs in human adrenocortical H295R cells in vitro. We recently reported that bone morphogenetic protein (BMP)-6, which is expressed in adrenocortical cells, enhances Ang II-but not potassium-induced aldosterone production in human adrenocortical cells. Accordingly, we examined the roles of BMP-6 in aldosterone breakthrough induced by long-term treatment with ARB. Ang II stimulated aldosterone production by adrenocortical cells. This Ang II stimulation was blocked by an ARB, candesartan. Interestingly, the candesartan effects on Ang II-induced aldosterone synthesis and CYP11B2 expression were attenuated in a course of candesartan treatment for 15 d. The impairment of candesartan effects on Ang II-induced aldosterone production was also observed in Ang II- or candesartanpretreated cells. Levels of Ang II type 1 receptor mRNA were not changed by chronic candesartan treatment. However, BMP-6 enhancement of Ang II- induced ERK1/2 signaling was resistant to candesartan. The BMP-6-induced Smad1, -5, and -8 phosphorylation, and BRE-Luc activity was augmented in the presence of Ang II and candesartan in the chronic phase. Chronic Ang II exposure decreased cellular expression levels of BMP-6 and its receptors activin receptor-like kinase-2 and activin type II receptor mRNAs. Cotreatment with candesartan reversed the inhibitory effects of Ang II on the expression levels of these mRNAs. The breakthrough phenomenon was attenuated by neutralization of endogenous BMP-6 and activin receptor-like kinase-2. Collectively, these data suggest that changes in BMP-6 availability and response may be involved in the occurrence of cellular escape from aldosterone suppression under chronic treatment with ARB.

en-copyright= kn-copyright= en-aut-name=OtaniHiroyuki en-aut-sei=Otani en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OtsukaFumio en-aut-sei=Otsuka en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InagakiKenichi en-aut-sei=Inagaki en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SuzukiJiro en-aut-sei=Suzuki en-aut-mei=Jiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyoshiTomoko en-aut-sei=Miyoshi en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KanoYoshihiro en-aut-sei=Kano en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=GotoJunko en-aut-sei=Goto en-aut-mei=Junko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OguraToshio en-aut-sei=Ogura en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MakinoHirofumi en-aut-sei=Makino en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences en-keyword=ENZYME-INHIBITOR THERAPY kn-keyword=ENZYME-INHIBITOR THERAPY en-keyword=CHRONIC HEART-FAILURE kn-keyword=CHRONIC HEART-FAILURE en-keyword=CONVERTING ENZYME kn-keyword=CONVERTING ENZYME en-keyword=HYPERTENSIVE PATIENTS kn-keyword=HYPERTENSIVE PATIENTS en-keyword=PLASMA-ALDOSTERONE kn-keyword=PLASMA-ALDOSTERONE en-keyword=GRANULOSA-CELLS kn-keyword=GRANULOSA-CELLS en-keyword=SYSTEM kn-keyword=SYSTEM en-keyword=SECRETION kn-keyword=SECRETION en-keyword=NEPHROPATHY kn-keyword=NEPHROPATHY en-keyword=HYPERTROPHY kn-keyword=HYPERTROPHY END start-ver=1.4 cd-journal=joma no-vol=152 cd-vols= no-issue=11 article-no= start-page=173 end-page=177 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200509 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=High-aspect-ratio copper-via-filling for three-dimensional chip stacking - II. Reduced electrodeposition process time en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Through-chip electrodes for three-dimensional packaging can offer short interconnection and reduced signal delay. Formation of suitable vias by electrodeposition into cavities presents a filling problem similar to that encountered in the damascene process. Because via dimensions for through-chip filling are larger and have a higher aspect ratio relative to features in damascene, process optimization requires modification of existing superconformal plating baths and plating parameters. In this study, copper filling of high-aspect-ratio through-chip vias was investigated and optimized with respect to plating bath composition and applied current wavetrain. Void-free vias 70 mu m deep and 10 mu m wide were formed in 60 min using additives in combination with pulse-reverse current and dissolved-oxygen enrichment. The effects of reverse current and dissolved oxygen on the performance of superfilling additives is discussed in terms of their effects on formation, destruction, and distribution of a Cu(I) thiolate accelerant. (c) 2005 The Electrochemical Society. All rights reserved.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YonezawaToshihiro en-aut-sei=Yonezawa en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MikamiDaisuke en-aut-sei=Mikami en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkuboToshikazu en-aut-sei=Okubo en-aut-mei=Toshikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaguchiYuichi en-aut-sei=Taguchi en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakahashiKenji en-aut-sei=Takahashi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=BarkeyDale P en-aut-sei=Barkey en-aut-mei=Dale P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Osaka Prefecture University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Toppan Printing Company, Limited affil-num=5 en-affil= kn-affil=Tsukuba Center Incorporated affil-num=6 en-affil= kn-affil=Tsukuba Center Incorporated affil-num=7 en-affil= kn-affil=University of New Hampshire en-keyword=plating baths kn-keyword=plating baths en-keyword=additives kn-keyword=additives END start-ver=1.4 cd-journal=joma no-vol=152 cd-vols= no-issue=10 article-no= start-page=688 end-page=691 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of annealing and hydrogen on properties of electrodeposited platinum electrode and lead-zirconate-titanate films for ferroelectric random access memory applications en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The selection of capacitor electrode materials for the nonvolatile ferroelectric random access memory is one of the most important issues because capacitor electrical characteristics are strongly influenced by the electrode materials. The lower Pt electrode was electrodeposited on the Ti seed/Pt seed layer. Two different thicknesses of Ti seed layer (5 and 15 nm) were adopted, and lead-zirconate-titanate (PZT) was deposited on the electrodeposited Pt. The Pt crystal orientation with a 5 nm Ti seed layer is much better than that with a 15 nm Ti seed layer, and the deposited PZT shows much better crystal orientation. Due to better crystal orientation of the PZT layer in the case of a 5 nm Ti seed layer, a Pt/PZT/Pt capacitor well-saturated D-V hysteresis loop was obtained whereas little current was observed in the large electric field. With the 15 nm Ti seed layer, numerous several-mu m-sized voids formed on the lower Pt electrode surface. With the 5 nm Ti seed layer, fewer voids formed on the lower Pt electrode surface. Glow discharge spectrometry measurement with a 15 nm Ti seed layer shows much higher H intensity than that with a 5 nm Ti seed layer, and the H peak coincides with the Ti peak. The H existing in the Ti seed layer must have transmitted into the PZT layer and deteriorated the PZT crystal orientation. (c) 2005 The Electrochemical Society.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeKeiji en-aut-sei=Watanabe en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AbeYuji en-aut-sei=Abe en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HajiJunko en-aut-sei=Haji en-aut-mei=Junko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShimizuMasaru en-aut-sei=Shimizu en-aut-mei=Masaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Osaka Prefecture University affil-num=2 en-affil= kn-affil=Osaka Prefecture University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=University of Hyogo en-keyword=chemical-vapor-deposition kn-keyword=chemical-vapor-deposition en-keyword=electrical-properties kn-keyword=electrical-properties END start-ver=1.4 cd-journal=joma no-vol=34 cd-vols= no-issue=10 article-no= start-page=1109 end-page=1119 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Polar and hydrogen-bonding effects of alcohols on the emission spectrum of styrene-triethylamine system en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The emission spectra of styrene (ST)-triethylamine (TEA) systems were measured under steady-state illumination conditions in some THF-protic solvent mixtures. The fluorescence spectrum of the ST-TEA system in THF consists of two bands (band A at 304 nm (fluorescence of ST) and band B at 460 nm (emission from an exciplex)). The intensity of band A increased and that of band B decreased with increasing amounts of protic solvents in THF-protic solvent mixtures. The increase in the intensity of band A was explained by the decrease in the concentration of free amine owing to the hydrogen-bonding interaction (or protonation) between TEA and protic solvents. The decrease in the intensity of band B was considered to be caused by the decrease in the concentration of free amine on the addition of protic solvents and the enhanced conversion of the exciplex to an ion pair with increasing solvent polarity. The polar effect was expressed as a function of the relative permittivity of the solution.

en-copyright= kn-copyright= en-aut-name=YamamotoShunzo en-aut-sei=Yamamoto en-aut-mei=Shunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=Solvent effect; Hydrogen-bonding; Polar effect; Exciplex; Fluorescence kn-keyword=Solvent effect; Hydrogen-bonding; Polar effect; Exciplex; Fluorescence END start-ver=1.4 cd-journal=joma no-vol=132 cd-vols= no-issue=12 article-no= start-page=2828 end-page=2834 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19858 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On a homogeneous electrochemical reaction of prussian blue/everitt's salt system en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Voltammetric, chronopotentiometric, and spectroelectrochemical studies qn the homogeneous-phase (single phase) reaction of Prussian blue (PB)/Everitt's salt (ES) system in KC1 aqueous solution were carried out as a model for understanding the homogeneous electrochemical reaction of manganese dioxide. Analytical results of voltammetric and chronopotentiometric studies on PB/ES system indicated that the electrode potential was represented by the empirical formula.

en-copyright= kn-copyright= en-aut-name=OhzukuTsutomu en-aut-sei=Ohzuku en-aut-mei=Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SawaiKeijiro en-aut-sei=Sawai en-aut-mei=Keijiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HiraiTaketsugu en-aut-sei=Hirai en-aut-mei=Taketsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Osaka City University en-keyword=manganese compounds kn-keyword=manganese compounds en-keyword=oxygen compounds kn-keyword=oxygen compounds en-keyword=electrochemical electrodes kn-keyword=electrochemical electrodes en-keyword=spectrochemical analysis kn-keyword=spectrochemical analysis en-keyword=electrochemistry kn-keyword=electrochemistry END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=8 article-no= start-page=2120 end-page=2124 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=200907 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The use of graphite oxide to produce mesoporous carbon supporting Pt, Ru, or Pd nanoparticles en-subtitle= kn-subtitle= en-abstract= kn-abstract=Mesoporous carbon having platinum, ruthenium or palladium nanoparticles on exfoliated graphene sheets were produced from graphite oxide (GO) and metal complexes. The Pt included carbon was made by heating of the intercalation compound including tetraammineplatinum (II) chloride monohydrate. Samples having Ru or Pd are producible by heating in nitrogen gas atmosphere using hexaammineruthenium (III) chloride or tetraamminepalladium (II) chloride monohydrate instead of Pt complex. The particle sizes of platinum, ruthenium, and palladium were, respectively, 1–3, 1–2, and 3–7 nm. The platinum- or palladium-containing sample showed catalytic activity for oxygen reduction. en-copyright= kn-copyright= en-aut-name=GotohKazuma en-aut-sei=Gotoh en-aut-mei=Kazuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawabataKoji en-aut-sei=Kawabata en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiiEiji en-aut-sei=Fujii en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MorishigeKunimitsu en-aut-sei=Morishige en-aut-mei=Kunimitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KinumotoTaro en-aut-sei=Kinumoto en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyazakiYuki en-aut-sei=Miyazaki en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IshidaHiroyuki en-aut-sei=Ishida en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Industrial Technology Center of Okayama Prefecture affil-num=3 en-affil= kn-affil=Industrial Technology Center of Okayama Prefecture affil-num=4 en-affil= kn-affil=Okayama University of Science affil-num=5 en-affil= kn-affil=Faculty of Engineering, Oita University affil-num=6 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue=22-24 article-no= start-page=3469 end-page=3477 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20061001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Line adsorption in a mean-field density functional model en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Recent ideas about the analog for a three-phase contact line of the Gibbs adsorption equation for interfaces are illustrated in a mean-field density-functional model. With $d¥tau$ the infinitesimal change in the line tension $¥tau$ that accompanies the infinitesimal changes $d¥mu_i$ in the thermodynamic field variables $¥mu_i$ and with $¥Lambda_i$ the line adsorptions, the sum $d¥tau + ¥Sigma ¥Lambda_i d¥mu_i$, unlike its surface analog, is not 0. An equivalent of this sum in the model system is evaluated numerically and analytically. A general line adsorption equation, which the model results illustrate, is derived.

en-copyright= kn-copyright= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WidomBenjamin en-aut-sei=Widom en-aut-mei=Benjamin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Cornell University en-keyword=line tension kn-keyword=line tension en-keyword=line adsorption kn-keyword=line adsorption en-keyword=adsorption equation kn-keyword=adsorption equation en-keyword=three-phase equilibria kn-keyword=three-phase equilibria en-keyword=partial wetting kn-keyword=partial wetting END start-ver=1.4 cd-journal=joma no-vol=145 cd-vols= no-issue=9 article-no= start-page=3007 end-page=3010 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=19989 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Shape evolution of electrodeposited bumps with deep cavity en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Electrodeposited bumps are the indispensable microconnectors for high-density interconnection in the latest microelectronics applications. The deep cavities are especially important for the solder bumps for ball grid arrays. This investigation discusses the relation between cavity shapes and current distributions of deep cavities. The role of convection and diffusion within the cavities is calculated at diffusion-limited overpotentials with numerical fluid dynamics computations. The current distributions become symmetric and peak profiles become sharper for the deeper cavities of large aspect ratios and of negative photoresist angles, theta. For 30 mu m cathode length, the current at the center is larger than that at the edges for photoresist angles of theta less than or equal to 0. For these deep cavities, the convection outside the cavity is not related to the current distribution and the current distribution is determined by the cavity shape. The mass transport within the deep cavities is controlled mainly by diffusion. This is because the convection outside the cavities is not effectively stirring inside the deep cavities.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FukuiKeisuke en-aut-sei=Fukui en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Himeji Institute of Technology en-keyword=copper bumps kn-keyword=copper bumps END start-ver=1.4 cd-journal=joma no-vol=123 cd-vols= no-issue=9 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050901 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Formation of ice nanotube with hydrophobic guests inside carbon nanotube en-subtitle= kn-subtitle= en-abstract= kn-abstract=A composite ice nanotube inside a carbon nanotube has been explored by molecular dynamics and grandcanonical Monte Carlo simulations. It is made from an octagonal ice nanotube whose hollow space contains hydrophobic guest molecules such as neon, argon, and methane. It is shown that the attractive interaction of the guest molecules stabilizes the ice nanotube. The guest occupancy of the hollow space is calculated by the same method as applied to clathrate hydrates. en-copyright= kn-copyright= en-aut-name=TanakaHideki en-aut-sei=Tanaka en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=ice nanotubes kn-keyword=ice nanotubes en-keyword=carbon nanotubes kn-keyword=carbon nanotubes END start-ver=1.4 cd-journal=joma no-vol=151 cd-vols= no-issue=7 article-no= start-page=514 end-page=518 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20046 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Crystal growth of electrolytic cu foil en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Electrolytic copper deposited on (100)(Cu) single crystals forms pyramidal crystals that grow parallel to the substrate at low current density without stirring. With stirring and higher current density, slanting platelet crystals with (111)(Cu) orientation are formed. Chloride and gelatin are typical additives for electrolytic copper foils and the electrolytic copper foils are formed on a titanium substrate with a stirring rate and high current density. With gelatin, triangular pyramidal crystals form with (111)(Cu) orientation. The triangle-shaped side planes of the crystals are the (100)(Cu), and platelet crystals exist along the (100)(Cu). With chloride and gelatin, triangular columnar crystals with the (110)(Cu) orientation are formed. The triangle-shaped side planes of the crystals are the (100)(Cu) and the platelet crystals again exist along the (100)(Cu). These platelet crystals are bound by macrosteps, and they are the growth sites. The morphology of electrolytic copper foils on the titanium substrate does not change with higher current densities and stirring rates. It was also found that chloride changes deposit orientations in the concentration range of less than 10 ppm. (C) 2004 The Electrochemical Society.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiHaruo en-aut-sei=Murakami en-aut-mei=Haruo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=sulfate kn-keyword=sulfate en-keyword=copper kn-keyword=copper en-keyword=baths kn-keyword=baths END start-ver=1.4 cd-journal=joma no-vol=162 cd-vols= no-issue=2 article-no= start-page=1322 end-page=1328 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200610 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Properties of a novel hard-carbon optimized to large size Lion secondary battery studied by 7Li NMR en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The state of lithium in a novel hard-carbon optimized to the anode of large size Li ion secondary battery, which has been recently commercialized, was investigated and compared with other existing hard-carbon samples by 7Li NMR method. The new carbon material showed a peak at 85 ppm with a shoulder signal at 7 ppm at room temperature in static NMR spectrum, and the former shifted to 210 ppm at 180 K. The latter at room temperature was attributed to Li doped in small particles contained in the sample. The new carbon sample showed weaker intensity of cluster-lithium signal than the other hard-carbon samples in NMR, which corresponded to a tendency of less "Constant Voltage" (CV) capacity in charge-discharge curves of electrochemical evaluation. Smaller CV capacity and initial irreversible capacity, which are the features of the novel hard-carbon, are considered to correspond to a blockade of the diffusion of Li into pore of carbon.

en-copyright= kn-copyright= en-aut-name=GotohKazuma en-aut-sei=Gotoh en-aut-mei=Kazuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MaedaMariko en-aut-sei=Maeda en-aut-mei=Mariko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagaiAisaku en-aut-sei=Nagai en-aut-mei=Aisaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=GotoAtsushi en-aut-sei=Goto en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TanshoMasataka en-aut-sei=Tansho en-aut-mei=Masataka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HashiKenjiro en-aut-sei=Hashi en-aut-mei=Kenjiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShimizuTadashi en-aut-sei=Shimizu en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IshidaHiroyuki en-aut-sei=Ishida en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Kureha Corporation affil-num=3 en-affil= kn-affil=Kureha Corporation affil-num=4 en-affil= kn-affil=National Institute for Materials Science affil-num=5 en-affil= kn-affil=National Institute for Materials Science affil-num=6 en-affil= kn-affil=National Institute for Materials Science affil-num=7 en-affil= kn-affil=National Institute for Materials Science affil-num=8 en-affil= kn-affil=Okayama University en-keyword=hard carbon kn-keyword=hard carbon en-keyword=Lithium kn-keyword=Lithium en-keyword=battery kn-keyword=battery en-keyword=7Li NMR kn-keyword=7Li NMR en-keyword=anode kn-keyword=anode en-keyword=electric vehicle kn-keyword=electric vehicle END start-ver=1.4 cd-journal=joma no-vol=148 cd-vols= no-issue=3 article-no= start-page=145 end-page=148 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Shape evolution of electrodeposited bumps into deep cavities en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Metal posts and finer pitch solder bumps are the indispensable microconnectors for chip size packaging and are formed by electrodeposition into deep cavities. It is difficult to stir inside these deep cavities. Natural convection due to density difference is effective in stirring inside cavity with 200 mum cathode width of aspect ratio of one. The bump shape increases toward lower side in a vertical cathode arrangement with placement angle of Theta = 90 degrees. This increase in bump height results from a collision of flow along the lower side of the resist sidewall which enlarges local current and thickens the lower edge of bumps. The effect of natural convection is also evident in the neighboring two cavities of 200 mum cathode width. The natural convection is not effective for cavities with less than 100 mum cathode width. The bump shapes become flat. Only diffusion occurs within these smaller than 100 mum cavities. (C) 2001 The Electrochemical Society. All rights reserved.

en-copyright= kn-copyright= en-aut-name=HayashiK en-aut-sei=Hayashi en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FukuiKeisuke en-aut-sei=Fukui en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaZenzo en-aut-sei=Tanaka en-aut-mei=Zenzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Himeji Institute of Technology affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=copper bumps kn-keyword=copper bumps END start-ver=1.4 cd-journal=joma no-vol=134 cd-vols= no-issue=11 article-no= start-page=2932 end-page=2934 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=19876 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Photoluminescence spectra and vibrational structures of the srs:ce3+ and srse:ce3+ phosphors en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The details of photoluminescence and excitation spectra of powder phosphors were obtained at various temperatures between 6 and 300 K. At 300 K, two emission bands originating from the 2T2g(5d)→2F7/2,2F5/2(4f) transitions are observed at 480 and 535 nm for SrS:Ce3+ and at 470 and 527 nm for SrSe:Ce3+, respectively. In the excitation spectra, bands corresponding to the 2F5/2(4f)→22T2g(5d) transition are observed at 433 (SrS:Ce3+) and 430 nm (SrSe:Ce3+), and plateaus due to the fundamental absorption of the host crystals in higher energy region. The vibrational structures on the emission and excitation bands are analyzed by the use of energy matrices in order to determine the coupling constant of spin-orbit interaction and the crystal field parameters V4(f), V6(f) for the 4f orbit.

en-copyright= kn-copyright= en-aut-name=YamashitaN en-aut-sei=Yamashita en-aut-mei=N kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MichitsujiY en-aut-sei=Michitsuji en-aut-mei=Y kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AsanoS en-aut-sei=Asano en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University of Science en-keyword=phosphors kn-keyword=phosphors en-keyword=strontium compounds kn-keyword=strontium compounds en-keyword=sulphur compounds kn-keyword=sulphur compounds en-keyword=selenium compounds kn-keyword=selenium compounds en-keyword=cerium kn-keyword=cerium en-keyword=photoluminescence kn-keyword=photoluminescence END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=3-4 article-no= start-page=233 end-page=239 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20064 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Elucidation of the role of the complex in hydride transfer reaction between methylene blue and 1-benzyl-1,4-dihydronictinamide by effect of γ-cyclodextrin en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The kinetics of the hydride transfer reaction between Methylene Blue (MB+) and　1-benzyl-1,4-dihydronicotinamide (BNAH) were studied in 10 % ethanol-90 % water mixed solvents containing β- and γ-cyclodextrins (β-CD and γ-CD). The pseudo-first order rate constant shows kinetic saturation at high initial concentration of BNAH. This indicates the formation of a complex between MB+ and BNAH. The reaction was suppressed by addition of β-CD, but enhanced by addition of γ-CD. MB+ and BNAH were separately accommodated within the β-CD cavity and the cavity walls may protect the activity site of the reactants. On the other hand, in the MB+-BNAH-γ-CD system, the inclusion of the complex between MB+ and BNAH with γ-CD occurred. This effect of γ-CD can distinguish between the productive and non-productive nature of the complex.

en-copyright= kn-copyright= en-aut-name=LiuYingin en-aut-sei=Liu en-aut-mei=Yingin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HotouchiNaoto en-aut-sei=Hotouchi en-aut-mei=Naoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SueishiYoshimi en-aut-sei=Sueishi en-aut-mei=Yoshimi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoShunzo en-aut-sei=Yamamoto en-aut-mei=Shunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=hydride transfer reaction kn-keyword=hydride transfer reaction en-keyword=reaction mechanism kn-keyword=reaction mechanism en-keyword=cyclodextrin kn-keyword=cyclodextrin en-keyword=inclusion complexes kn-keyword=inclusion complexes en-keyword=1-benzyl-1 kn-keyword=1-benzyl-1 en-keyword=4-dihydronicotinamide kn-keyword=4-dihydronicotinamide en-keyword=Methylene Blue kn-keyword=Methylene Blue END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=14 article-no= start-page=145503 end-page=1 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20044 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Bridging the gap between small clusters and nanodroplets: spectroscopic study and computer simulation of carbon dioxide solvated with helium atoms. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

High resolution infrared spectra of HeN–CO2 clusters with N up to about 20 have been studied in the region of the CO2 v3 fundamental band. The B rotational constant initially drops as expected for a normal molecule, reaching a minimum for N = 5. Its subsequent rise for N = 6 to 11 can be interpreted as the transition from a normal (though floppy) molecule to a quantum solvation regime. For N > 13, the B value becomes approximately constant with a value about 17% larger than that measured in much larger helium nanodroplets. Quantum Monte Carlo calculations of pure rotational spectra are in excellent agreement with the measured B in this size range, and complement the experimental study with detailed structural information. For larger cluster size (N = 30-50) the simulations show a clear sign of convergence towards the nanodroplet B value.

en-copyright= kn-copyright= en-aut-name=TangJian en-aut-sei=Tang en-aut-mei=Jian kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=McKellarRobert A en-aut-sei=McKellar en-aut-mei=Robert A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MezzacapoFabio en-aut-sei=Mezzacapo en-aut-mei=Fabio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoroniSaverio en-aut-sei=Moroni en-aut-mei=Saverio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Steacie Institute for Molecular Science affil-num=3 en-affil= kn-affil=Università di Roma La Sapienza affil-num=4 en-affil= kn-affil=Università di Roma La Sapienza en-keyword=infrared spectroscopy kn-keyword=infrared spectroscopy en-keyword=helium cluster kn-keyword=helium cluster en-keyword=molecular superfluidity kn-keyword=molecular superfluidity en-keyword=computer simulation kn-keyword=computer simulation END start-ver=1.4 cd-journal=joma no-vol=826 cd-vols= no-issue=1 article-no= start-page=1 end-page=5 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20065 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cl-35 NQR study of lattice dynamic and magnetic property of a crystalline coordination polymer {CuCA(phz)(H2O)2}n en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Copper(II) compounds {CuCA(phz)(H2O)2}n (H2CA = chloranilic acid, phz = phenazine) having a layer structure of -CuCA(H2O)2- polymer chains and phenazine was studied by 35Cl nuclear quadrupole resonance (NQR). The single NQR line observed at 35.635 MHz at 261.5 K increased to 35.918 MHz at 4.2 K. The degree of reduction of electric field gradient due to lattice vibrations was similar to that of chloranilic acid crystal. Temperature dependence of spin-lattice relaxation time, T1, of the 35Cl NQR signal below 20 K, between 20 and 210 K, and above 210 K, was explained by 1) a decrease of effective electron-spin density caused by antiferromagnetic interaction, 2) a magnetic interaction between Cl nuclear-spin and electron-spins on paramagnetic Cu(II) ions, and 3) an increasing contribution from reorientation of ligand molecules, respectively. The electron spin-exchange parameter |J| between the neighboring Cu(II) electrons was estimated to be 0.33 cm−1 from the T1 value of the range 20−210 K. Comparing this value with that of J = −1.84 cm−1 estimated from the magnetic susceptibility, it is suggested that the magnetic dipolar 2 coupling with the electron spins on Cu(II) ions must be the principal mechanism for the 35Cl NQR spin-lattice relaxation of {CuCA(phz)(H2O)2}n but a delocalization of electron spin over the chloranilate ligand have to be taken into account.

en-copyright= kn-copyright= en-aut-name=GotohKazuma en-aut-sei=Gotoh en-aut-mei=Kazuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TeraoTakeshi en-aut-sei=Terao en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AsajiTetsuo en-aut-sei=Asaji en-aut-mei=Tetsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Nihon University affil-num=3 en-affil= kn-affil=Nihon University en-keyword=crystalline coordination polymer kn-keyword=crystalline coordination polymer en-keyword=paramagnetic kn-keyword=paramagnetic en-keyword=nuclear resonance kn-keyword=nuclear resonance en-keyword=spin-lattice relaxation kn-keyword=spin-lattice relaxation en-keyword=spin exchange kn-keyword=spin exchange END start-ver=1.4 cd-journal=joma no-vol=145 cd-vols= no-issue=3 article-no= start-page=840 end-page=844 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=19983 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Current evolution of electrodeposited copper bumps with photoresist angle en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We report the current distribution of copper bumps with photoresist sidewall angles. The role of outer diffusion, vortices, and penetration flow within the cavity is discussed, with numerical fluid dynamics computed in order to prevent side bumping. The current distributions were calculated at the diffusion controlled overpotential. The mass transfer-limited current distribution showed that a zero or negative angle reduced diffusion from the outer surroundings and enhanced vortex formation at the cathode corners. Reduction in the diffusion and enhancement in vortices reduced current at the cathode corners and prevented side bumping.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FukuiKeisuke en-aut-sei=Fukui en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Himeji Institute of Technology en-keyword=shape evolution kn-keyword=shape evolution en-keyword=foil kn-keyword=foil en-keyword=transport kn-keyword=transport END start-ver=1.4 cd-journal=joma no-vol=123 cd-vols= no-issue=11 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20059 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Infrared spectra of seeded hydrogen clusters: (para-H2)N-N2O and (ortho-H2)N-N2O, N=2-13 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

High-resolution infrared spectra of clusters containing para-H2 and/or ortho-H2 and a single nitrous oxide molecule are studied in the 2225-cm–1 region of the 1 fundamental band of N2O. The clusters are formed in pulsed supersonic jet expansions from a cooled nozzle and probed using a tunable infrared diode laser spectrometer. The simple symmetric rotor-type spectra generally show no resolved K structure, with prominent Q-branch features for ortho-H2 but not para-H2 clusters. The observed vibrational shifts and rotational constants are reported. There is no obvious indication of superfluid effects for para-H2 clusters up to N=13. Sharp transitions due to even larger clusters are observed, but no definite assignments are possible. Mixed (para-H2)N–(ortho-H2)M–N2O cluster line positions can be well predicted by linear interpolation between the corresponding transitions of the pure clusters.

en-copyright= kn-copyright= en-aut-name=TangJian en-aut-sei=Tang en-aut-mei=Jian kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=McKellarRobert A en-aut-sei=McKellar en-aut-mei=Robert A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Steacie Institute for Molecular Science en-keyword=vibrational kn-keyword=vibrational en-keyword=rotational kn-keyword=rotational en-keyword=infrared spectroscopy kn-keyword=infrared spectroscopy en-keyword=gas phase kn-keyword=gas phase en-keyword=superfluidity kn-keyword=superfluidity END start-ver=1.4 cd-journal=joma no-vol=151 cd-vols= no-issue=4 article-no= start-page=250 end-page=255 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20042 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Role of additives for copper damascene electrodeposition experimental study on inhibition and acceleration effects en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The role of copper Damascene additives is discussed based on electrodeposit morphology on a through-mask cathode, field emission-Auger (FE-Auger), quartz crystal microbalance (QCM), and electrochemical measurements. Adsorbed particles, several tens of nanometers in diameter were observed on copper-electrodeposited surfaces by field emission-scanning electron microscopy (FE-SEM). These particles show a stronger oxygen intensity peak by FE-Auger spectrum than bare electrodeposited surfaces. The QCM frequency deviation did not increase with time in the CuSO4 and H2SO4 bath without polyethylene glycol (PEG) and chloride ion (Cl-) additives. When the substrates were immersed in the bath with these additives, the deviation markedly increased with time. Numerous PEG molecules were observed by FE-SEM immersed after 1000 s. The current density remained constant at a low value for the bath with PEG and Cl- additives. The current density started to increase markedly with time just after adding 1 ppm of bis(3-sulfopropyl) disulfide (SPS). Numerous PEG molecules were present on the electrodeposits before adding SPS. No PEG molecules, however, remained on the surface once SPS was added to the bath. The current density increased with narrower opening widths of the through-mask cathode. Despite this increase, the deposit cross sections on narrower opening widths of 2 and 10 mum were flat and no curvatures were found. Hence, the deposit curvature is not the origin of the acceleration effect. (C) 2004 The Electrochemical Society.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsumotoToshiaki en-aut-sei=Matsumoto en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeKeiji en-aut-sei=Watanabe en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=polyethylene-glycol kn-keyword=polyethylene-glycol en-keyword=deposition kn-keyword=deposition END start-ver=1.4 cd-journal=joma no-vol=151 cd-vols= no-issue=7 article-no= start-page=514 end-page=518 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20046 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Development of cu-core pb-free solder bumps en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Cu-core Pb-free solder bumps were developed by electrodeposition in combination with photolithography. A bump with a pitch of 100 mum and a diam of 50 mum was successfully achieved with dry film resist (80 mum thick) under optimum process conditions, and the bumps reached the target height of 50 6 5 mm. The adoption of a dummy pattern improved the height uniformity of the bumps. Pull test results showed that the shear strength of the Cu bump (around 45 gf) was obtained by the soft-etching pretreatment of the Cu foil surface. Sn-Zn, Sn-Ag, Sn-Cu, and Sn-Bi platings were deposited as Pb-free solders with near-eutectic compositions. In particular, the interface properties of Sn-Zn solder plating/Cu were investigated after reflowing in this study, as well as the effect of Ni plating as an under ball metallurgy layer on compound formation at the interface. It was found that a compound of Y-Cu5Zn8 layer existed in the interface of the reflowed Sn-Zn plating/Cu. The Ni plating layer inhibited the formation of the interface Y-Cu5Zn8 compound with reflowing. (C) 2004 The Electrochemical Society.

en-copyright= kn-copyright= en-aut-name=MuDaobin en-aut-sei=Mu en-aut-mei=Daobin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MaedaJunpei en-aut-sei=Maeda en-aut-mei=Junpei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=electrodeposited copper bumps kn-keyword=electrodeposited copper bumps en-keyword=shape evolution kn-keyword=shape evolution en-keyword=lead kn-keyword=lead en-keyword=alloys kn-keyword=alloys END start-ver=1.4 cd-journal=joma no-vol=136 cd-vols= no-issue=9 article-no= start-page=2606 end-page=2608 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=19881130 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on the acid-base properties of the AIBr3-NaBr melts en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The acid-base properties of the ZnBr[2]-NaBr melts at 623 K were investigated on the basis of the electromotive force measurements of a zinc-zinc concentration cell. The following two chemical equilibria were postulated to describe the acid-base character of the melts ZBnr[2]+Br[-]=ZnBr[3][-] K[1] ZnBr[3][-]+Br[-] =ZnBr[4][2+] K[2] The equilibrium constants K[1] and K[2], were determined to be 5.0×10, and 1.0×10[2], respectively, at 623 K

en-copyright= kn-copyright= en-aut-name=HayashiHidetaka en-aut-sei=Hayashi en-aut-mei=Hidetaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HayashiN en-aut-sei=Hayashi en-aut-mei=N kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakeharaZ en-aut-sei=Takehara en-aut-mei=Z kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KatagiriA en-aut-sei=Katagiri en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Kyoto University affil-num=3 en-affil= kn-affil=Kyoto University affil-num=4 en-affil= kn-affil=Kyoto University en-keyword=liquid mixtures kn-keyword=liquid mixtures en-keyword=aluminium compounds kn-keyword=aluminium compounds en-keyword=sodium compounds kn-keyword=sodium compounds en-keyword=bromine compounds kn-keyword=bromine compounds en-keyword=chemical equilibrium kn-keyword=chemical equilibrium en-keyword=electrochemical analysis kn-keyword=electrochemical analysis en-keyword=thermodynamic properties kn-keyword=thermodynamic properties en-keyword=thermochemistry kn-keyword=thermochemistry en-keyword=electric potential kn-keyword=electric potential END start-ver=1.4 cd-journal=joma no-vol=140 cd-vols= no-issue=2 article-no= start-page=362 end-page=365 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19932 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Codeposition of a-alumina particles from acid copper sulfate bath en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The purpose of this work is to elucidate the factors affecting the codeposition of a-alumina particles with copper from an acid copper sulfate bath. The effect of current density and particle concentration in the bath on the amount of codeposited a-alumina were determined. In order to evaluate the surface-chemical properties of the particles, the zeta-potential of a-alumina particles in the plating bath was determined by means of the streaming potential method. The amounts of adsorbed Cu 2+ and SO2( from the plating bath were also determined. The amount of codeposited a-alumina particles was found to be greater than those reported in literature, in spite of the negative zeta-potential values of a-alumina. This indicates that the electrostatic interaction between the particles and the cathode is not the essential factor of the codeposition process. It is considered that the amount of adsorbed Cu 2+ on the a-alumina particle plays an important role in the codeposition behavior.

en-copyright= kn-copyright= en-aut-name=HayashiHidetaka en-aut-sei=Hayashi en-aut-mei=Hidetaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IzumiShin-ya en-aut-sei=Izumi en-aut-mei=Shin-ya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TariIsao en-aut-sei=Tari en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=Deposition kn-keyword=Deposition en-keyword=Kinetics kn-keyword=Kinetics END start-ver=1.4 cd-journal=joma no-vol=137 cd-vols= no-issue=11 article-no= start-page=3642 end-page=3647 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=19903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of heat-treatments on electrical properties of boron-doped silicon crystals en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The effects of heat-treatments around 1000°Ｃand subsequent annealing on the electrical properties of boron-doped silicon have been studied by electrical conductivity, Hall effect, and deep-level transient spectroscopy measurements. The high-temperature heat-treatments always induced net densities of donors. Four recovery stages, stages I-IV, of heat-treatment- induced donors were observed on isochronal annealing up to 400°Ｃ Conductivity changes in these stages can be explained as described below by the reactions of interstitial iron (Fei), its pair (Fe1Bs)with substitutional boron (Bs), and two unknown donors (D1, D2). That is, stage I (25°-100°Ｃ): D1→sink and Fei + Bs→FeiBs, stage II (100°-150°Ｃ)： FeiBs→Fei + Bs, stage III (200°-250°Ｃ):D2→sink, stage IV (250°-350°Ｃ)Fei→precipitation. Heat-treatments in an oxygen atmosphere greatly reduced the introduction of Fei and FeiBs in comparison with an argon atmosphere and mainly introduced D1 and D2 donors. The density of D2 was dependent on the heat-treatment temperature, while that of D1 showed almost no dependence. In stage I, D, was annihilated by first-order kinetics with an activation energy of 0.8 eV. It was indicated that DI and D2 have no relations to iron, copper, oxygen, nor carbon. Though their origins are still unidentified, there may be some interstitial impurities. In stage IV, Fei is suggested to precipitate at oxygen precipitates and dislocation loops formed by high-temperature heat-treatments. As to the application to iron gettering in the device fabrication process, it is proposed that annealing around 300°Ｃ is most suitable as the final heat-treatment step to remove iron and related defects from active regions of devices. Silicon wafers receive complex heat-treatments at various.

en-copyright= kn-copyright= en-aut-name=KamiuraY. en-aut-sei=Kamiura en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoF. en-aut-sei=Hashimoto en-aut-mei=F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YonetaM. en-aut-sei=Yoneta en-aut-mei=M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=silicon kn-keyword=silicon en-keyword=crystals kn-keyword=crystals en-keyword=doping kn-keyword=doping en-keyword=boron kn-keyword=boron en-keyword=heat treatment kn-keyword=heat treatment en-keyword=annealing kn-keyword=annealing en-keyword=electrical conductivity measurement kn-keyword=electrical conductivity measurement END start-ver=1.4 cd-journal=joma no-vol=152 cd-vols= no-issue=11 article-no= start-page=173 end-page=177 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200509 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=High-aspect-ratio copper via filling used for three-dimensional chip stacking en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Through-chip electrodes for three-dimensional packaging can offer short interconnection and reduced signal delay. Formation of suitable vias by electrodeposition into cavities presents a filling problem similar to that encountered in the damascene process. Because via dimensions for through-chip filling are larger and have a higher aspect ratio relative to features in damascene, process optimization requires modification of existing superconformal plating baths and plating parameters. In this study, copper filling of high-aspect-ratio through-chip vias was investigated and optimized with respect to plating bath composition and applied current wavetrain. Void-free vias 70 mu m deep and 10 mu m wide were formed in 60 min using additives in combination with pulse-reverse current and dissolved-oxygen enrichment. The effects of reverse current and dissolved oxygen on the performance of superfilling additives is discussed in terms of their effects on formation, destruction, and distribution of a Cu(I) thiolate accelerant. (c) 2005 The Electrochemical Society. All rights reserved.

en-copyright= kn-copyright= en-aut-name=SunJian-Jun en-aut-sei=Sun en-aut-mei=Jian-Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkamuraTakuji en-aut-sei=Okamura en-aut-mei=Takuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhSeungJin en-aut-sei=Oh en-aut-mei=SeungJin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TomisakaManabu en-aut-sei=Tomisaka en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YonemuraHitoshi en-aut-sei=Yonemura en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HoshinoMasataka en-aut-sei=Hoshino en-aut-mei=Masataka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakahashiKenji en-aut-sei=Takahashi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Tsukuba Research Center affil-num=6 en-affil= kn-affil=Tsukuba Research Center affil-num=7 en-affil= kn-affil=Tsukuba Research Center affil-num=8 en-affil= kn-affil=Tsukuba Research Center en-keyword=plating baths kn-keyword=plating baths en-keyword=additives kn-keyword=additives END start-ver=1.4 cd-journal=joma no-vol=69 cd-vols= no-issue=1 article-no= start-page=147 end-page=152 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Observation of micropores in hard-carbon using Xe-129 NMR porosimetry en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The existence of micropores and the change of surface structure in pitch-based hard-carbon in xenon atmosphere were demonstrated using Xe-129 NMR. For high-pressure (4.0 MPa) Xe-129 NMR measurements, the hard-carbon samples in Xe gas showed three peaks at 27, 34 and 210 ppm. The last was attributed to the xenon in micropores (<1 nm) in hard-carbon particles. The NMR spectrum of a sample evacuated at 773 K and exposed to 0.1 MPa Xe gas at 773 K for 24 h showed two peaks at 29 and 128 ppm, which were attributed, respectively, to the xenon atoms adsorbed in the large pores (probably mesopores) and micropores of hard-carbon. With increasing annealing time in Xe gas at 773 K, both peaks shifted and merged into one peak at 50 ppm. The diffusion of adsorbed xenon atoms is very slow, probably because the transfer of molecules or atoms among micropores in hard-carbon does not occur readily. Many micropores are isolated from the outer surface. For that reason, xenon atoms are thought to be adsorbed only by micropores near the surface, which are easily accessible from the surrounding space.

en-copyright= kn-copyright= en-aut-name=GotohKazuma en-aut-sei=Gotoh en-aut-mei=Kazuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UedaTakahiro en-aut-sei=Ueda en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OmiHironori en-aut-sei=Omi en-aut-mei=Hironori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=EguchiTaro en-aut-sei=Eguchi en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MaedaMariko en-aut-sei=Maeda en-aut-mei=Mariko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyaharaMichihisa en-aut-sei=Miyahara en-aut-mei=Michihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AisakuNagai en-aut-sei=Aisaku en-aut-mei=Nagai kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IshidaHiroyuki en-aut-sei=Ishida en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=The Museum of Osaka University affil-num=3 en-affil= kn-affil=The Museum of Osaka University affil-num=4 en-affil= kn-affil=The Museum of Osaka University affil-num=5 en-affil= kn-affil=Research Center, Kureha Corporation affil-num=6 en-affil= kn-affil=Research Center, Kureha Corporation affil-num=7 en-affil= kn-affil=Research Center, Kureha Corporation affil-num=8 en-affil= kn-affil=Okayama University en-keyword=amorphous materials kn-keyword=amorphous materials en-keyword=microporous materials kn-keyword=microporous materials en-keyword=nuclear magnetic resonance (NMR) kn-keyword=nuclear magnetic resonance (NMR) END start-ver=1.4 cd-journal=joma no-vol=144 cd-vols= no-issue=2 article-no= start-page=466 end-page=470 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19972 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Shape evolution of electrodeposited copper bumps with high peclet numbers en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We report the shape evolution of initial copper bumps at Peclet numbers higher than a hundred. The role of vortices and of penetration flow within the cavity was discussed with numerical fluid dynamics computation to obtain a bump with a single hump at the center. The current distributions, or flux profiles, were calculated at the diffusion controlled overpotential and were compared with the electrodeposited bump shapes. For the 100 mu m cavity width, the vortices increase at the upstream corners with Peclet numbers 1410 and 7311. The vortices are the local resistance of mass transfer to the cathode. These vortices cause the hollows in flux profiles at the upstream corner with these Peclet numbers. The penetration flow collides with the photoresist sidewall and the vortices decrease at downstream corners. These decreased vortices cause the increase in flux profile at downstream corners. For a 30 pm cavity width a single large vortex forms for the higher Peclet number 44,500 and a single hump in flux is achieved.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FukuiKeisuke en-aut-sei=Fukui en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokoyamaMitsunori en-aut-sei=Yokoyama en-aut-mei=Mitsunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShinoharaKunio en-aut-sei=Shinohara en-aut-mei=Kunio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Himeji Institute of Technology affil-num=3 en-affil= kn-affil=University of Hokkaido affil-num=4 en-affil= kn-affil=University of Hokkaido en-keyword=foil kn-keyword=foil en-keyword=transport kn-keyword=transport en-keyword=cavities kn-keyword=cavities END start-ver=1.4 cd-journal=joma no-vol=147 cd-vols= no-issue=7 article-no= start-page=2611 end-page=2613 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200007 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Electrodeposition of Zinc-SiO2 composite en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The incorporation mechanism of SiO2 particles into zinc electrodeposit is discussed. The SiO2 particles precipitate in two ways on the (00-1)(eta) of zinc electrodeposit: by lined up particles along the laterally growing macrosteps on the (00.1)(eta) and by randomly dispersed particles on the (00.1)(eta). These particles incorporate into the electrodeposits by following two processes. The sidewalls of particles are incorporated into the macrosteps at the edge of (00.1)(eta). The bottom of randomly dispersed particles are incorporated into the (00.1)(eta) probably by the atomic steps. (C) 2000 The Electrochemical Society.

en-copyright= kn-copyright= en-aut-name=KondoKazuo en-aut-sei=Kondo en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhgishiAtsufumi en-aut-sei=Ohgishi en-aut-mei=Atsufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaZennosuke en-aut-sei=Tanaka en-aut-mei=Zennosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=atomic-force microscopy kn-keyword=atomic-force microscopy en-keyword=iron binary alloy kn-keyword=iron binary alloy en-keyword=zinc kn-keyword=zinc en-keyword=morphology microstructure kn-keyword=morphology microstructure END start-ver=1.4 cd-journal=joma no-vol=140 cd-vols= no-issue=2 article-no= start-page=386 end-page=389 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19932 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on the acid-base properties of the ZnBr2NaBr molten salt system en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The acid-base properties of the ZnBr2-NaBr melts at 623 K were investigated on the basis of the electromotive force measurements of a zinc-zinc concentration cell. The following two chemical equilibria were postulated to describe the acid-base character of the melts.

en-copyright= kn-copyright= en-aut-name=HayashiHidetaka en-aut-sei=Hayashi en-aut-mei=Hidetaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UnoKiyofumi en-aut-sei=Uno en-aut-mei=Kiyofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakeharaZen-ichiro en-aut-sei=Takehara en-aut-mei=Zen-ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KatagiriAkira en-aut-sei=Katagiri en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Kyoto University affil-num=3 en-affil= kn-affil=Kyoto University affil-num=4 en-affil= kn-affil=Kyoto University en-keyword=alcl3-nacl melts kn-keyword=alcl3-nacl melts en-keyword=equilibria kn-keyword=equilibria END start-ver=1.4 cd-journal=joma no-vol=19 cd-vols= no-issue=3 article-no= start-page=465 end-page=475 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20038 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of an increase in patient copayments on medical service demands of the insured in japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Objectives:To examine quantitatively the effects of an increase in patient copayments from 10% to 20% on the demand for medical services in Japan.
Methods: The subjects of the study were the employees insured by the 1,797 health insurance societies, belonging to the National Federation of Health Insurance Societies, in 1996 and 1998. Indicators of medical service demands analyzed include the inpatient, outpatient, and dental case rates, the number of serviced days per case, the medical cost per day and the medical cost per insured.
Results: When the effects of an increase in patient copayments from 10% to 20% were evaluated, taking into account the average age, the average monthly salary, the total number, the gender (male-tofemale) ratio and the dependent ratio of the insured, the estimated change in the case rate was −6.96% for inpatient, −4.79% for outpatient, and −5.77% for dental care. The estimated change in the number of serviced day per case was −4.66% for inpatient, −5.67% for outpatient, and −1.82% for dental care. The estimated change in the medical cost per day was −3.15% for inpatient, −13.00% for outpatient, and −11.48% for dental care. The estimated change in the medical cost per insured was −14.08% for inpatient, −21.54% for outpatient, and −18.11% for dental care.
Conclusions: The increase in patient copayments from 10% to 20% enabled insurers to substantially reduce medical costs by cost shifting from the insurer to the insured, with resultant changes in the case rate and the number of service days per case.

en-copyright= kn-copyright= en-aut-name=BabazonoAkira en-aut-sei=Babazono en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsudaToshihide en-aut-sei=Tsuda en-aut-mei=Toshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoEiji en-aut-sei=Yamamoto en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MinoYoshio en-aut-sei=Mino en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=UneHiroshi en-aut-sei=Une en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HillmanAlan L. en-aut-sei=Hillman en-aut-mei=Alan L. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Kyushu University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University of Science affil-num=4 en-affil= kn-affil=Osaka Prefecture University affil-num=5 en-affil= kn-affil=Fukuoka University affil-num=6 en-affil= kn-affil=University of Pennsylvania, US en-keyword=Copayments kn-keyword=Copayments en-keyword=Health insurance kn-keyword=Health insurance en-keyword=Health policy kn-keyword=Health policy en-keyword=Japan kn-keyword=Japan END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue=2 article-no= start-page=228 end-page=233 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20054 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of the increase in co-payments from 20 to 30 percent on the compliance rate of patients with hypertension or diabetes mellitus in the Employed Health Insurance System en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Objectives: How to contain medical expenditures is a universal problem. The Japanese government has increased patient co-payments to control it. The purpose of this study is to clarify whether the increase in co-payments to 30 percent prevented patients with hypertension or diabetes mellitus from receiving necessary care in the Employee Health Insurance System.
Methods: The subjects were 211 patients with hypertension and 66 patients with diabetes mellitus who regularly visited physicians from October 2001 to March 2002 and were defined as a cohort that needed health care, and their medical indicators were examined between April and September 2002 (prestage) and between April and September 2003 (poststage).
Results: In the hypertensive patients with no complications, the compliance rate was 89.9 percent and 88.0 percent in the prestage, and poststage, respectively, showing no significant change. In the hypertensive patients with complications, the compliance rate was 90.5 percent and 92.1 percent in the prestage and poststage, respectively, showing no significant change. In the diabetic patients with complications, the compliance rate was 77.5 percent and 79.2 percent, in the prestage and poststage, respectively, with no significant change. In the diabetic patients with no complications, however, the compliance rate was 83.7 percent and 66.7 percent, in the prestage and poststage, respectively. A significant decrease was observed among diabetic patients without complications.
Conclusions: Increasing co-payments reduced necessary preventive care in diabetic patients without complications.

en-copyright= kn-copyright= en-aut-name=BabazonoAkira en-aut-sei=Babazono en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyazakiMotonobu en-aut-sei=Miyazaki en-aut-mei=Motonobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ImatohTakuya en-aut-sei=Imatoh en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UneHiroshi en-aut-sei=Une en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamamotoEiji en-aut-sei=Yamamoto en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TsudaToshihide en-aut-sei=Tsuda en-aut-mei=Toshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TanakaKiyoshi en-aut-sei=Tanaka en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TaniharaShinichi en-aut-sei=Tanihara en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Kyushu University affil-num=2 en-affil= kn-affil=Fukuoka University affil-num=3 en-affil= kn-affil=Fukuoka University affil-num=4 en-affil= kn-affil=Fukuoka University affil-num=5 en-affil= kn-affil=Okayama University of Science affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Japan Health Food and Nutritional Food Association affil-num=8 en-affil= kn-affil=Shimane University en-keyword=Co-payments kn-keyword=Co-payments en-keyword=Compliance kn-keyword=Compliance en-keyword=Hypertension kn-keyword=Hypertension en-keyword=Diabetes mellitus kn-keyword=Diabetes mellitus en-keyword=Health policy kn-keyword=Health policy END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=5 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20059 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Electron- and neutrino-nucleus scattering in the impulse approximation regime en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A quantitative understanding of the weak nuclear response is a prerequisite for the analyses of neutrino experiments such as K2K and MiniBOONE, which measure energy and angle of the muons produced in neutrino-nucleus interactions in the energy range 0.5-3 GeV and reconstruct the incident neutrino energy to determine neutrino oscillations. In this paper we discuss theoretical calculations of electron- and neutrino-nucleus scattering, carried out within the impulse approximation scheme using realistic nuclear spectral functions. Comparison between electron scattering data and the calculated inclusive cross section of oxygen, at beam energies ranging between 700 and 1200 MeV, show that the Fermi gas model, widely used in the analysis of neutrino oscillation experiments, fails to provide a satisfactory description of the measured cross sections, and inclusion of nuclear dynamics is needed.

en-copyright= kn-copyright= en-aut-name=BenherOmar en-aut-sei=Benher en-aut-mei=Omar kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FarinaNicola en-aut-sei=Farina en-aut-mei=Nicola kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakamuraHiroki en-aut-sei=Nakamura en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakudaMakoto en-aut-sei=Sakuda en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SekiRyoichi en-aut-sei=Seki en-aut-mei=Ryoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Ist Nazl Fis Nucl affil-num=2 en-affil= kn-affil=University of Roma La Sapienza affil-num=3 en-affil= kn-affil=Waseda University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=California State University en-keyword=monte-carlo calculations kn-keyword=monte-carlo calculations en-keyword=spectal-function kn-keyword=spectal-function en-keyword=proton propagation kn-keyword=proton propagation en-keyword=e kn-keyword=e en-keyword=e'p kn-keyword=e'p en-keyword=peactions kn-keyword=peactions en-keyword=cross-section kn-keyword=cross-section en-keyword=light-nucle? kn-keyword=light-nucle? en-keyword=matter kn-keyword=matter en-keyword=o-16 kn-keyword=o-16 en-keyword=transparency kn-keyword=transparency en-keyword=oscillation kn-keyword=oscillation END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=5 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20063 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Search for anomalous couplings in top decay at hadron colliders en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We present a quantitative study on sensitivities to the top-decay anomalous couplings, taking into account realistic experimental conditions expected at Tevatron and LHC. A double angular distribution of W and charged lepton in the top-decay is analyzed, using t<(t)<($)over bar> events in the leptons+jets channel. In order to improve sensitivities to the anomalous couplings, we apply two techniques: (i) We use a likelihood fitting method for full kinematical reconstruction of each top event. (ii) We develop a new effective spin reconstruction method for leptonically-decayed top quarks; this method does not require spin information of the antitop side. For simplicity, we neglect couplings of right-handed bottom quark as well as CP violating couplings. The 95% C.L. estimated bound on a ratio of anomalous couplings reads -0.81 < f(2)/f(1)<-0.70, -0.12 < f(2)/f(1)< 0.14 using 1000 reconstructed top events at Tevatron, while -0.74 < f(2)/f(1)<-0.72, -0.01 < f(2)/f(1)< 0.01 is expected with 100 k reconstructed top events at LHC, where only statistical errors are taken into account. A two-fold ambiguity in the allowed range remains when the number of events exceeds a few hundred.

en-copyright= kn-copyright= en-aut-name=TsunoS en-aut-sei=Tsuno en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakanoI en-aut-sei=Nakano en-aut-mei=I kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaR en-aut-sei=Tanaka en-aut-mei=R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=quark pair production kn-keyword=quark pair production en-keyword=order qcd correction kn-keyword=order qcd correction en-keyword=spin correlations kn-keyword=spin correlations en-keyword=linear kn-keyword=linear en-keyword=colliders kn-keyword=colliders en-keyword=charged leptons kn-keyword=charged leptons en-keyword=width kn-keyword=width en-keyword=thvatron kn-keyword=thvatron en-keyword=collisions kn-keyword=collisions en-keyword=threshold kn-keyword=threshold END start-ver=1.4 cd-journal=joma no-vol=456 cd-vols= no-issue=4 article-no= start-page=687 end-page=691 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080626 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Barley plasma membrane intrinsic proteins (PIP aquaporins) as water and CO2 transporters en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We identified barley aquaporins and demonstrated that one, HvPIP2;1, transports water and CO2. Regarding water homeostasis in plants, regulations of aquaporin expression were observed in many plants under several environmental stresses. Under salt stress, a number of plasma membrane-type aquaporins were down-regulated, which can prevent continuous dehydration resulting in cell death. The leaves of transgenic rice plants that expressed the largest amount of HvPIP2;1 showed a 40% increase in internal CO2 conductance compared with leaves of wild-type rice plants. The rate of CO2 assimilation also increased in the transgenic plants. The goal of our plant aquaporin research is to determine the key aquaporin species responsible for water and CO2 transport, and to improve plant water relations, stress tolerance, CO2 uptake or assimilation, and plant productivity via molecular breeding of aquaporins.

en-copyright= kn-copyright= en-aut-name=KatsuharaMaki en-aut-sei=Katsuhara en-aut-mei=Maki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HanbaYuko T. en-aut-sei=Hanba en-aut-mei=Yuko T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Research Institute for Bioresources, Okayama University affil-num=2 en-affil= kn-affil=Center for Bioresource Field Science, Kyoto Institute of Technology en-keyword=barley kn-keyword=barley en-keyword=CO2 kn-keyword=CO2 en-keyword=plant aquaporins kn-keyword=plant aquaporins en-keyword=salt stress kn-keyword=salt stress en-keyword=water transport kn-keyword=water transport END start-ver=1.4 cd-journal=joma no-vol=353 cd-vols= no-issue=1-2 article-no= start-page=28 end-page=34 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080529 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Albumin-conjugated PEG liposome enhances tumor distribution of liposomal doxorubicin in rats en-subtitle= kn-subtitle= en-abstract= kn-abstract=

To evaluate the effect of coupling of recombinant human serum albumin (rHSA) onto the surface of poly(ethylene glycol)-modified liposorne (PEG liposome) on the in vivo disposition characteristics of liposomal doxorubicin (DXR), the pharmacokinetics and tissue distribution of DXR were evaluated after intravenous administration of rHSA-modified PEG (rHSA/PEG) liposomal DXR into tumor-bearing rats. rHSA/PEG liposome prepared using a hetero-bifunctional cross-linker, N- succinimidyl 3-(2-pyridyldithio) propionate (SPDP), efficiently encapsulated DXR (over 95%). rHSA/PEG liposomal DXR showed longer blood-circulating property than PEG liposornal DXR and the hepatic and splenic clearances of rHSA/PEG liposornal DXR were significantly smaller than those of PEG liposomal DXR. It was also demonstrated that the disposition of DXR to the heart, one of the organs for DXR-related side-effects, was significantly smaller than free DXR. Furthermore, the tumor accumulation of rHSA/PEG liposomal DXR was significantly larger than that of PEG liposomal DXR. The "therapeutic index", a criterion for therapeutic outcome, for rHSA/PEG fiposornal DXR was significantly higher than PEG liposomal DXR. These results clearly indicate that rHSA-conjugation onto the surface of PEG liposome would be a useful approach to increase the effectiveness and safety of PEG liposomal DXR.

en-copyright= kn-copyright= en-aut-name=YokoeJun-ichi en-aut-sei=Yokoe en-aut-mei=Jun-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SakuragiShiho en-aut-sei=Sakuragi en-aut-mei=Shiho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoKayoko en-aut-sei=Yamamoto en-aut-mei=Kayoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TeragakiTakuya en-aut-sei=Teragaki en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OgawaraKen-ichi en-aut-sei=Ogawara en-aut-mei=Ken-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HigakiKazutaka en-aut-sei=Higaki en-aut-mei=Kazutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KatayamaNaohisa en-aut-sei=Katayama en-aut-mei=Naohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KaiToshiya en-aut-sei=Kai en-aut-mei=Toshiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SatoMakoto en-aut-sei=Sato en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KimuraToshikiro en-aut-sei=Kimura en-aut-mei=Toshikiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Pharmaceutical Research Division, NIPRO Corporation affil-num=2 en-affil= kn-affil=Pharmaceutical Research Division, NIPRO Corporation affil-num=3 en-affil= kn-affil=Pharmaceutical Research Division, NIPRO Corporation affil-num=4 en-affil= kn-affil=Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University affil-num=5 en-affil= kn-affil=Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University affil-num=6 en-affil= kn-affil=bDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University affil-num=7 en-affil= kn-affil=Pharmaceutical Research Division, NIPRO Corporation affil-num=8 en-affil= kn-affil=Pharmaceutical Research Division, NIPRO Corporation affil-num=9 en-affil= kn-affil=Pharmaceutical Research Division, NIPRO Corporation affil-num=10 en-affil= kn-affil=Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University en-keyword=Recombinant human serum albumin (rHSA) kn-keyword=Recombinant human serum albumin (rHSA) en-keyword=PEG liposome kn-keyword=PEG liposome en-keyword=Doxorubicin kn-keyword=Doxorubicin en-keyword=Tumor-bearing rats kn-keyword=Tumor-bearing rats en-keyword=Passive targeting kn-keyword=Passive targeting END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=1 article-no= start-page=77 end-page=81 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20062 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Positive association of AKT1 haplotype to Japanese methamphetamine use disorder en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Recent evidence suggests that the AKT1-GSK3Β signalling cascade partially mediates dopaminedependentbehaviours. In relation to the pathophysiology of schizophrenia or methamphetamine (Meth)use disorder, AKT1 is a good candidate gene for such conditions. For schizophrenia, positive associationsof SNPs and AKT1 haplotypes were reported in US and Japanese samples. To evaluate the association between AKT1 and Meth-use disorder, we conducted a case-control study of Japanese samples (182 patients and 437 controls). A positive association between a SNP and haplotypes was found, and the ‘signal’ SNP was the same SNP found to be associated with US schizophrenia, but not with Japanese schizophrenia. Our results indicate that AKT1 may play a possible role in the development of Meth-use disorder. Further investigation of these associations, together with evidence from previous animal studies, may open the way to elucidation of the pathophysiology of this condition.

en-copyright= kn-copyright= en-aut-name=IkedaMasashi en-aut-sei=Ikeda en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IwataNakao en-aut-sei=Iwata en-aut-mei=Nakao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuzukiTatsuyo en-aut-sei=Suzuki en-aut-mei=Tatsuyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KitajimaTsuyoshi en-aut-sei=Kitajima en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamanouchiYoshio en-aut-sei=Yamanouchi en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KinoshitaYoko en-aut-sei=Kinoshita en-aut-mei=Yoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SekineYoshimoto en-aut-sei=Sekine en-aut-mei=Yoshimoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IyoMasaomi en-aut-sei=Iyo en-aut-mei=Masaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=HaranoMutsuo en-aut-sei=Harano en-aut-mei=Mutsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KomiyamaTokutaro en-aut-sei=Komiyama en-aut-mei=Tokutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=YamadaMitsuhiko en-aut-sei=Yamada en-aut-mei=Mitsuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=SoraIchiro en-aut-sei=Sora en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=UjikeHiroshi en-aut-sei=Ujike en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=InadaToshiya en-aut-sei=Inada en-aut-mei=Toshiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=OzakiNorio en-aut-sei=Ozaki en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= affil-num=1 en-affil= kn-affil=Fujita Health University, Aichi affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Fujita Health University affil-num=4 en-affil= kn-affil=Fujita Health University, Aichi affil-num=5 en-affil= kn-affil=Fujita Health University affil-num=6 en-affil= kn-affil=Fujita Health University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University affil-num=11 en-affil= kn-affil=Okayama University affil-num=12 en-affil= kn-affil=Okayama University affil-num=13 en-affil= kn-affil=Okayama University affil-num=14 en-affil= kn-affil=Okayama University affil-num=15 en-affil= kn-affil=Okayama University en-keyword=Dopamine-dependent behaviours kn-keyword=Dopamine-dependent behaviours en-keyword=linkage disequilibrium kn-keyword=linkage disequilibrium en-keyword=substance-related disorders. kn-keyword=substance-related disorders. END start-ver=1.4 cd-journal=joma no-vol=343 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080522 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evaluation of intramuscular lateral distribution profile of topically administered acetaminophen in rats en-subtitle= kn-subtitle= en-abstract= kn-abstract=

To clarify to what extent topically administered drug molecules horizontally permeate into tissues surrounding the administration site, the intramuscular lateral concentration profile of acetaminophen was investigated in vivo using the microdialysis method in rats. When acetaminophen was intramuscularly administered for 6 hr in a pinpoint manner at a constant rate of 3 μg/min, it was clearly detected in the muscle surrounding the administration site, being 17.5 μg/ml when measured at a 2 mm distance from the administration site. The concentration in the muscle was decreased as the distance increased, and those measured at 5 mm and 40 mm were 0.35 μg/ml and 0.09 μg/ml, respectively. In addition, it was shown that the concentration in the muscle at 40 mm reflected the compound’s concentration in plasma, but not the compound’s horizontal permeation from the administration site. With these observations, the intramuscular distribution profile of acetaminophen was numerically characterized according to Fick’s law. As a result, it was revealed that horizontal permeation is the primary process accountable for the increased intramuscular concentration only in the area adjacent to the administration site, and the radius of the adjacent area was calculated to be 5.80 mm for acetaminophen.

en-copyright= kn-copyright= en-aut-name=KurosakiYuji en-aut-sei=Kurosaki en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TagawaMasahiro en-aut-sei=Tagawa en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OmotoAkiho en-aut-sei=Omoto en-aut-mei=Akiho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SuitoHiroshi en-aut-sei=Suito en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KomoriYukiko en-aut-sei=Komori en-aut-mei=Yukiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AibaTetsuya en-aut-sei=Aiba en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Environmental Sciences, Okayama University affil-num=5 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=7 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=Microdialysis kn-keyword=Microdialysis en-keyword=Intramuscular concentration profile kn-keyword=Intramuscular concentration profile en-keyword=Drug disposition kn-keyword=Drug disposition en-keyword=Acetaminophen kn-keyword=Acetaminophen en-keyword=Pharmacokinetics kn-keyword=Pharmacokinetics en-keyword=Drug delivery kn-keyword=Drug delivery END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=3 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080520 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Chronic coadministration of carbamazepine together with imipramine produces antidepressant-like effects in an ACTH-induced animal model of treatment–resistant depression: Involvement of 5-HT 2A receptors? en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The use of carbamazepine has been reported to be an effective treatment for severe depression. We have already shown that the antidepressant-like effects of tricyclic antidepressants in the rat forced swim test (FST) are blocked by chronic treatment with adrenocorticotropic hormone (ACTH). In the present study, we examined the effect of the chronic administration of carbamazepine on the FST and the wet-dog shakes induced by (+/-)-1-(2,5-dimethoxy-4-iodophenyl)-2-aiminopropane (DOI), a 5-HT2A receptor agonist, in ACTH-treated rats. Chronic administration of carbamazepine did not affect the duration of immobility in saline-treated and ACTH-treated rats. The reduction of immobility, induced by chronic administration of imipramine, was blocked by treatment with ACTH. When carbamazepine was administered concurrently with imipramine, we observed a significant decrease in immobility in rats treated with ACTH. Chronic ACTH treatment increased the number of the wet-dog shakes induced by DOI. This effect of ACTH was significantly increased by the coadministration of carbamazepine and imipramine. These results suggest that the use of carbamazepine together with tricyclic antidepressants had the effect of reducing immobility time in the FST in a tricyclic antidepressant-treatment-resistant depressive model induced by chronic ACTH treatment.

en-copyright= kn-copyright= en-aut-name=KitamuraYoshihisa en-aut-sei=Kitamura en-aut-mei=Yoshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkiyamaKozue en-aut-sei=Akiyama en-aut-mei=Kozue kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KitagawaKouhei en-aut-sei=Kitagawa en-aut-mei=Kouhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShibataKazuhiko en-aut-sei=Shibata en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SuemaruKatsuya en-aut-sei=Suemaru en-aut-mei=Katsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ArakiHiroaki en-aut-sei=Araki en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SendoToshiaki en-aut-sei=Sendo en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University Medical School affil-num=3 en-affil= kn-affil=Okayama University Medical School affil-num=4 en-affil= kn-affil=Okayama University Medical School affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Ehime University Medical School affil-num=7 en-affil= kn-affil=Ehime University Medical School affil-num=8 en-affil= kn-affil=Okayama University Medical School affil-num=9 en-affil= kn-affil=Okayama University Medical School en-keyword=Imipramine kn-keyword=Imipramine en-keyword=Carbamazepine kn-keyword=Carbamazepine en-keyword=ACTH kn-keyword=ACTH en-keyword=5-HT2A receptor kn-keyword=5-HT2A receptor en-keyword=Forced swim test kn-keyword=Forced swim test en-keyword=Wet-dog shakes kn-keyword=Wet-dog shakes en-keyword=Treatment–resistant kn-keyword=Treatment–resistant END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=8 article-no= start-page=887 end-page=893 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=200412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Generation of active fragments from human zymogens in the bradykinin-generating cascade by extracellular proteases from Vibrio vulnificus and V. parahaemolyticus en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Vibrio vulnificus is an opportunistic human pathogen causing septicemia, and the infection is characterized by formation of the edematous skin lesions on limbs. This pathogenic species secretes a thermolysin-like metalloprotease as a virulence determinant. The metalloprotease was confirmed to activate human factor XII-plasma kallikrein-kinin cascade that results in liberation of bradykinin, a chemical mediator enhancing the vascular permeability, from high-molecular weight kininogen. Namely, the metalloprotease showed to generate active fragments by cleavage of Arg-Ile, Arg-Val or Gly-Leu peptide bond in human zymogens (plasma prekallikrein and factor XII). In spite of induction of the sufficient vascular permeability-enhancing and edema-forming reaction in the guinea pig model, a serine protease from V. parahaemolyticus, a human pathogen causing primarily watery diarrhea, showed far less ability to activate and to cleave the human zymogens. These results in part may explain why only V. vulnificus often causes serious edematous skin damages in humans.

en-copyright= kn-copyright= en-aut-name=MiyoshiShin-ichi en-aut-sei=Miyoshi en-aut-mei=Shin-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeHirofumi en-aut-sei=Watanabe en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawaseTomoka en-aut-sei=Kawase en-aut-mei=Tomoka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamadaHidenori en-aut-sei=Yamada en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShinodaSumio en-aut-sei=Shinoda en-aut-mei=Sumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=vibrio vulnificus kn-keyword=vibrio vulnificus en-keyword=vibrio parahaemolyticus kn-keyword=vibrio parahaemolyticus en-keyword=protease kn-keyword=protease en-keyword=factor XII kn-keyword=factor XII en-keyword=plasma prekallikrein kn-keyword=plasma prekallikrein END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=3 article-no= start-page=508 end-page=513 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Induction of cytolytic activity and interferon-gamma production in murine natural killer cells by polymyxins B and E en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Natural killer (NK) cells are the primary effector cells of the innate immune system and have well-established roles in tumor rejection and resistance to viruses, bacteria and certain parasites. There is a need for more specific immune modulators of NK cell activity that tack the wide-ranging side effects of NK cell-stimulatory interleukins. The polycationic antibiotic polymyxin B (PMB) has been shown to have a unique ability to enhance activities of some immune cells, independent of its antibiotic properties. Here we report that both PMB and its analog potymyxin E (PME) markedly enhanced the activity of NK cells enriched from the murine spleen. Maximal activation of NK cell activity was obtained after 24 h of incubation with PMB at a dose of 300 mu g/ml. PMB nonapeptide, one of the two PMB domains, and PME methanesulfonate, the negatively charged derivative of PME, had little effect on NK cell activity. PMB induced interferon (IFN)-gamma and tumor necrosis factor-a production in NK cells. Proliferation of NK cells in vitro was significantly stimulated by being incubated with PMB. Administration of PMB to mice for 7 consecutive days stimulated splenic NK cell activity and increased NK cell populations in the spleen. These results suggest that the polycationic antibiotics PMB and PME may up-regulate innate and adaptive immune responses by induction of NK cell activity and IFN-gamma production.

en-copyright= kn-copyright= en-aut-name=ZhongMing en-aut-sei=Zhong en-aut-mei=Ming kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KadotaYusuke en-aut-sei=Kadota en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShimizuYoshio en-aut-sei=Shimizu en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=GohdaEiichi en-aut-sei=Gohda en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Bizen Chemical Co., Ltd affil-num=4 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=polymyxin B (PMB) kn-keyword=polymyxin B (PMB) en-keyword=polymyxin E (PME) kn-keyword=polymyxin E (PME) en-keyword=NK cells kn-keyword=NK cells en-keyword=IFN-gamma kn-keyword=IFN-gamma END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=23 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200511 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Opacity effect on extreme ultraviolet radiation from laser-produced tin plasmas en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Opacity effects on extreme ultraviolet (EUV) emission from laser-produced tin (Sn) plasma have been experimentally investigated. An absorption spectrum of a uniform Sn plasma generated by thermal x rays has been measured in the EUV range (9-19 nm wavelength) for the first time. Experimental results indicate that control of the optical depth of the laser-produced Sn plasma is essential for obtaining high conversion to 13.5 nm-wavelength EUV radiation; 1.8% of the conversion efficiency was attained with the use of 2.2 ns laser pulses.

en-copyright= kn-copyright= en-aut-name=FujiokaShinsuke en-aut-sei=Fujioka en-aut-mei=Shinsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishimuraHiroaki en-aut-sei=Nishimura en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishiharaKatsunobu en-aut-sei=Nishihara en-aut-mei=Katsunobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SasakiAkira en-aut-sei=Sasaki en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SunaharaAtsushi en-aut-sei=Sunahara en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OkunoTomoharu en-aut-sei=Okuno en-aut-mei=Tomoharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=UedaNobuyoshi en-aut-sei=Ueda en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=AndoTsuyoshi en-aut-sei=Ando en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TaoYezheng en-aut-sei=Tao en-aut-mei=Yezheng kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ShimadaYoshinori en-aut-sei=Shimada en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=HashimotoKazuhisa en-aut-sei=Hashimoto en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=YamauraMichiteru en-aut-sei=Yamaura en-aut-mei=Michiteru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=ShigemoriKeisuke en-aut-sei=Shigemori en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=NakaiMitsuo en-aut-sei=Nakai en-aut-mei=Mitsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=NagaiKeiji en-aut-sei=Nagai en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=NorimatsuTakayoshi en-aut-sei=Norimatsu en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=NishikawaTakeshi en-aut-sei=Nishikawa en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=MiyanagaNoriaki en-aut-sei=Miyanaga en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= en-aut-name=IzawaYasukazu en-aut-sei=Izawa en-aut-mei=Yasukazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=19 ORCID= en-aut-name=MimaKunioki en-aut-sei=Mima en-aut-mei=Kunioki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=20 ORCID= affil-num=1 en-affil= kn-affil=Osaka University affil-num=2 en-affil= kn-affil=Osaka University affil-num=3 en-affil= kn-affil=Osaka University affil-num=4 en-affil= kn-affil=Advanced Photon Research Center affil-num=5 en-affil= kn-affil=Institute for Laser Technology affil-num=6 en-affil= kn-affil=Osaka University affil-num=7 en-affil= kn-affil=Osaka University affil-num=8 en-affil= kn-affil=Osaka University affil-num=9 en-affil= kn-affil=Osaka University affil-num=10 en-affil= kn-affil=Institute for Laser Technology affil-num=11 en-affil= kn-affil=Institute for Laser Technology affil-num=12 en-affil= kn-affil=Institute for Laser Technology affil-num=13 en-affil= kn-affil=Osaka University affil-num=14 en-affil= kn-affil=Osaka University affil-num=15 en-affil= kn-affil=Osaka University affil-num=16 en-affil= kn-affil=Osaka University affil-num=17 en-affil= kn-affil=Okayama University affil-num=18 en-affil= kn-affil=Osaka University affil-num=19 en-affil= kn-affil=Osaka University affil-num=20 en-affil= kn-affil=Osaka University en-keyword=emission kn-keyword=emission en-keyword=targets kn-keyword=targets en-keyword=lithography kn-keyword=lithography en-keyword=fusion kn-keyword=fusion END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=14 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20054 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Observation of the acceleration of a single bunch by using the induction device in the kek proton synchrotron en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A single rf bunch in the KEK proton synchrotron was accelerated with an induction acceleration method from the injection energy of 500 MeV to 5 GeV.

en-copyright= kn-copyright= en-aut-name=TakayamaKen en-aut-sei=Takayama en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KosekiKunio en-aut-sei=Koseki en-aut-mei=Kunio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TorikaiKota en-aut-sei=Torikai en-aut-mei=Kota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TokuchiAkira en-aut-sei=Tokuchi en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakamuraEiji en-aut-sei=Nakamura en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ArakidaYoshio en-aut-sei=Arakida en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShimosakiYoshito en-aut-sei=Shimosaki en-aut-mei=Yoshito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=WakeMasayoshi en-aut-sei=Wake en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KounoTadaaki en-aut-sei=Kouno en-aut-mei=Tadaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=HoriokaKazuhiko en-aut-sei=Horioka en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IgarashiSusumu en-aut-sei=Igarashi en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=IwashitaTaiki en-aut-sei=Iwashita en-aut-mei=Taiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=KawasakiAtsushi en-aut-sei=Kawasaki en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=KishiroJun-ichi en-aut-sei=Kishiro en-aut-mei=Jun-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=SakudaMakoto en-aut-sei=Sakuda en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=SatoHikaru en-aut-sei=Sato en-aut-mei=Hikaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=ShihoMakoto en-aut-sei=Shiho en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=ShirakataMasashi en-aut-sei=Shirakata en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= en-aut-name=SuenoTsuyoshi en-aut-sei=Sueno en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=19 ORCID= en-aut-name=ToyamaTakeshi en-aut-sei=Toyama en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=20 ORCID= en-aut-name=WatanabeMasao en-aut-sei=Watanabe en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=21 ORCID= en-aut-name=YamaneIsao en-aut-sei=Yamane en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=22 ORCID= affil-num=1 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=2 en-affil= kn-affil=Graduate University for Advanced Studies affil-num=3 en-affil= kn-affil=Kyushu University affil-num=4 en-affil= kn-affil=Nichicon Corporation affil-num=5 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=6 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=7 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=8 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=9 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=10 en-affil= kn-affil=Tokyo Institute of Technology affil-num=11 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=12 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=13 en-affil= kn-affil=Nichicon Corporation affil-num=14 en-affil= kn-affil=Japan Atomic Energy Research Institute affil-num=15 en-affil= kn-affil=Okayama University affil-num=16 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=17 en-affil= kn-affil=Japan Atomic Energy Research Institute affil-num=18 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=19 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=20 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK affil-num=21 en-affil= kn-affil=Japan Atomic Energy Research Institute affil-num=22 en-affil= kn-affil=High Energy Accelerator Research Organization, KEK END start-ver=1.4 cd-journal=joma no-vol=413 cd-vols= no-issue=4-6 article-no= start-page=379 end-page=383 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050926 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fabrication of a logic gate circuit based on ambipolar field-effect transistors with thin films of C60 and pentacene en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Ambipolar field-effect transistor (FET) devices were fabricated with a heterostructure of C60 and pentacene, and their p- and n-channel field-effect mobilities were studied as a function of thickness of pentacene thin-films. The observed dependences of the μ values were interpreted in terms of the morphology of the thin films and the band structure of C60/pentacene heterostructure. A complementary metal-oxide-semiconductor (CMOS) circuit was fabricated by integration of two ambipolar FETs, aiming at realization of a new CMOS inverter circuit composed of FETs with the same device structure. The gain of 4, the threshold voltage of 85 V, and the complex output characteristics were explained on the basis of the properties of the component FET devices.

en-copyright= kn-copyright= en-aut-name=KuwaharaEiji en-aut-sei=Kuwahara en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KusaiHaruka en-aut-sei=Kusai en-aut-mei=Haruka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NaganoTakayuki en-aut-sei=Nagano en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=5 en-affil= kn-affil=Department of Chemistry, Okayama University en-keyword=Band structure kn-keyword=Band structure en-keyword=Carbon kn-keyword=Carbon en-keyword=CMOS integrated circuits kn-keyword=CMOS integrated circuits en-keyword=Field effect transistors kn-keyword=Field effect transistors en-keyword=Logic gates kn-keyword=Logic gates en-keyword=Thin films kn-keyword=Thin films en-keyword=Threshold voltage kn-keyword=Threshold voltage en-keyword=Band structures kn-keyword=Band structures en-keyword=Logic gate circuits kn-keyword=Logic gate circuits en-keyword=N-channel field-effective mobilities kn-keyword=N-channel field-effective mobilities en-keyword=Pentacene kn-keyword=Pentacene en-keyword=Logic circuits kn-keyword=Logic circuits END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=22 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Electronic structure and spontaneous internal field around nonmagnetic impurities in spin-triplet chiral p-wave superconductors en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The electronic structure around an impurity in spin-triplet p-wave superconductors is studied by the Bogoliubov-de Gennes theory on a tight-binding model, where we have chosen sin p(x)+i sin p(y)-wave or sin(p(x)+p(y))+i sin(-p(x)+p(y))-wave states, which are considered to be candidates for the pairing state in Sr2RuO4. We calculate the spontaneous current and the local density of states around the impurity and discuss the difference between the two types of pairing. We propose that it is possible to discriminate the two pairing states by studying the spatial dependence of the magnetic field around a pair of impurities.

en-copyright= kn-copyright= en-aut-name=TakigawaMitsuaki en-aut-sei=Takigawa en-aut-mei=Mitsuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IchiokaMasanori en-aut-sei=Ichioka en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KurokiKazuhiko en-aut-sei=Kuroki en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanakaYukio en-aut-sei=Tanaka en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Hokkaido University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=University of Electro-Communications affil-num=4 en-affil= kn-affil=Nagoya University en-keyword=unconventional superconductors kn-keyword=unconventional superconductors en-keyword=sr2ru04 kn-keyword=sr2ru04 en-keyword=states kn-keyword=states en-keyword=scattering kn-keyword=scattering en-keyword=junction kn-keyword=junction en-keyword=shift kn-keyword=shift en-keyword=phase kn-keyword=phase en-keyword=model kn-keyword=model en-keyword=gap kn-keyword=gap END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=6 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070814 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Line and boundary tensions on approach to the wetting transition en-subtitle= kn-subtitle= en-abstract= kn-abstract=A mean-field density-functional model often used in the past in the study of line and boundary tensions at wetting and prewetting transitions is reanalyzed by extensive numerical calculations, approaching the wetting transition much more closely than had previously been possible. The results are what are now believed to be definitive for the model. They include strong numerical evidence for the presence of the logarithmic factors predicted by theory both in the mode of approach of the prewetting line to the triple-point line at the point of the first-order wetting transition and in the line tension itself on approach to that point. It is also demonstrated with convincing numerical precision that the boundary tension on the prewetting line and the line tension on the triple-point line have a common limiting value at the wetting transition, again as predicted by theory. As a by product of the calculations, in the model's symmetric three-phase state, far from wetting, it is found that certain properties of the model's line tension and densities are almost surely given by simple numbers arising from the symmetries, but proving that these are exact for the model remains a challenge to analytical theory. en-copyright= kn-copyright= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WidomB. en-aut-sei=Widom en-aut-mei=B. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Baker Laboratory, Cornell University en-keyword=SURFACE PHASES kn-keyword=SURFACE PHASES en-keyword=FLUID PHASES kn-keyword=FLUID PHASES en-keyword=SUBSTRATE kn-keyword=SUBSTRATE en-keyword=ADSORPTION kn-keyword=ADSORPTION en-keyword=INTERFACE kn-keyword=INTERFACE en-keyword=CONTACT kn-keyword=CONTACT en-keyword=MODEL kn-keyword=MODEL en-keyword=ICE kn-keyword=ICE END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=6 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20052 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Anisotropic diamagnetic response in type-II superconductors with gap and fermi-surface anisotropies en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The effects of anisotropic gap structures on a diamagnetic response are investigated in order to demonstrate that the field-angle-resolved magnetization [M-L (chi)] measurement can be used as a spectroscopic method to detect gap structures. Our microscopic calculation based on the quasiclassical Eilenberger formalism reveals that M-L (chi) in a superconductor with a fourfold gap displays a fourfold oscillation reflecting the gap and Fermi-surface anisotropies, and the sign of this oscillation changes at a field between H-c1 and H-c2. As a prototype of unconventional superconductors, magnetization data for borocarbides are also discussed.

en-copyright= kn-copyright= en-aut-name=AdachiH en-aut-sei=Adachi en-aut-mei=H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiranovicP en-aut-sei=Miranovic en-aut-mei=P kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IchiokaM en-aut-sei=Ichioka en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MachidaK en-aut-sei=Machida en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=University of Montenegro affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=ginzburg-landau equations kn-keyword=ginzburg-landau equations en-keyword=density-of-states kn-keyword=density-of-states en-keyword=virial-theorem kn-keyword=virial-theorem en-keyword=magnetization kn-keyword=magnetization en-keyword=dependence kn-keyword=dependence en-keyword=symmetry kn-keyword=symmetry en-keyword=luni2b2c kn-keyword=luni2b2c en-keyword=yni2b2c kn-keyword=yni2b2c END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=22 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20056 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Metallic phase in the metal-intercalated higher fullerene Rb8.8(7)C84 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A new material of higher fullerene, RbxC84, was synthesized by intercalating Rb metal into C-84 crystals. The RbxC(84) crystals showed a simple cubic (sc) structure with lattice constant, a, of 16.82 (2) angstrom at 6.5 K, and 16.87 (2) angstrom at 295 K. The Rietveld refinements were achieved with the space group, Pa (3) over bar, based on a model that the C-2 axis of D2d-C84 aligned along [111]. The sample composition was determined to be Rb-8.8(7) C-84. The ESR spectrum at 303 K was composed of a broad peak with peak-to-peak linewidth Delta H-pp of 220 G, and a narrow peak with Delta H-pp of 24 G. Temperature dependence of the broad peak clearly showed a metallic behavior. The metallic behavior was discussed based on a theoretical calculation. This finding of new metallic phase in a higher fullerene is the first step for a development of new types of fullerene materials with novel physical properties such as superconductivity.

en-copyright= kn-copyright= en-aut-name=RikiishiYoshie en-aut-sei=Rikiishi en-aut-mei=Yoshie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KashinoYoko en-aut-sei=Kashino en-aut-mei=Yoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KusaiHaruka en-aut-sei=Kusai en-aut-mei=Haruka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakabayashiYasuhiro en-aut-sei=Takabayashi en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KuwaharaEiji en-aut-sei=Kuwahara en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KambeTakashi en-aut-sei=Kambe en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakenobuTaishi en-aut-sei=Takenobu en-aut-mei=Taishi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IwasaYoshihiro en-aut-sei=Iwasa en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=MizorogiNaomi en-aut-sei=Mizorogi en-aut-mei=Naomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=NagaseShigeru en-aut-sei=Nagase en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=OkadaSusumu en-aut-sei=Okada en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=5 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=6 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=7 en-affil= kn-affil=Department of Physics, Okayama University affil-num=8 en-affil= kn-affil=CREST, Japan Science and Technology Agency affil-num=9 en-affil= kn-affil=CREST, Japan Science and Technology Agency affil-num=10 en-affil= kn-affil=Institute for Molecular Science affil-num=11 en-affil= kn-affil=Institute for Molecular Science affil-num=12 en-affil= kn-affil=Institute of Physics and Center for Computational Science, University of Tsukuba END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=2 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=20001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Annealing effects on the magnetic and structural properties of single-crystal TDAE-C-60 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Annealing effects on the magnetic and structural properties of single-crystal TDAE-C-60 are investigated. When a crystal is well-annealed at 350 K, ferromagnetic ordering takes place below 16 K, though no magnetic phase transition is shown in as-grown crystal. The saturated magnetization was obtained to be 0.9+/-0.1 mu(B) per C-60. It was first found that the well-annealed crystal shows a structural phase transition around 180 K, probably associated with the orientational ordering of C-60 molecules. On the other hand, the as-grown crystal undergoes no structural phase transition at least down to 30 K while the motion of C-60 molecules is restricted below around 150 K. The possible relation between the low-temperature structure and the magnetic ordering is discussed.

en-copyright= kn-copyright= en-aut-name=KambeTakashi en-aut-sei=Kambe en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NogamiYoshio en-aut-sei=Nogami en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaKokichi en-aut-sei=Oshima en-aut-mei=Kokichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Physics, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Physics, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Physics, Faculty of Science, Okayama University END start-ver=1.4 cd-journal=joma no-vol=401 cd-vols= no-issue= article-no= start-page=218 end-page=221 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080629 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Formation of low-resistivity region in p-Si substrate of SiGe/Si episystem by remote-hydrogen plasma treatment en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We have studied effects of hydrogen treatment on the resistivity profile of the SiGe/Si episystem by spreading resistance (SR) method. In this paper, we present experimental findings that hydrogen treatment reduces the resistivity at a specific part in the Si substrate region. This position was confirmed to be under the interface between SiGe and Si that emerged on the bevel surface during hydrogen treatment. We investigated the depth of resistivity-reduced regions which was formed by various hydrogenating conditions and found that the region was extended to the same depth as the penetration depth of hydrogen. We concluded that the low-resistivity region was formed under the influence of hydrogen introduced from bevel surface. We attributed this resistivity reduction to formation of some defects which originally existed at the interface and diffused into Si substrate with hydrogen.

en-copyright= kn-copyright= en-aut-name=YamashitaYoshifumi en-aut-sei=Yamashita en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SakamotoYoshifumi en-aut-sei=Sakamoto en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KamiuraYoichi en-aut-sei=Kamiura en-aut-mei=Yoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IshiyamaTakeshi en-aut-sei=Ishiyama en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University en-keyword=SiGe/Si kn-keyword=SiGe/Si en-keyword=Hydroge. Resistivity reduction kn-keyword=Hydroge. Resistivity reduction en-keyword=Interface kn-keyword=Interface END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=6 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20062 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Resonant inelastic x-ray scattering at the oxygen k resonance of nio:nonlocal charge transfer and double-singlet excitations en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We report high-resolution polarization-dependent resonant inelastic x-ray scattering (RIXS) at the O K resonance of NiO showing a rich excitation spectrum. We perform multisite Ni6O19 cluster model calculations, revealing that solid state effects are substantial. We identify a nonlocal charge transfer excitation at 4-5 eV and double-singlet creation at 1.75 eV, both exhibiting significant scattering geometry dependence. Apart from an intense band of local charge transfer excitations (above 5 eV) also dd excitations at 1 eV are observed. Finally, we point out that O K RIXS of correlated metal oxides allows a quantitative and consistent determination of the charge transfer energy Delta and the Hund coupling energy J(H).

en-copyright= kn-copyright= en-aut-name=DudaL C en-aut-sei=Duda en-aut-mei=L C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SchmittT en-aut-sei=Schmitt en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MangnusonM en-aut-sei=Mangnuson en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ForsbergJ en-aut-sei=Forsberg en-aut-mei=J kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OlssonA en-aut-sei=Olsson en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NordgrenJ. en-aut-sei=Nordgren en-aut-mei=J. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkadaK en-aut-sei=Okada en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KotaniA en-aut-sei=Kotani en-aut-mei=A kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Uppsala University affil-num=2 en-affil= kn-affil=Uppsala University affil-num=3 en-affil= kn-affil=Uppsala University affil-num=4 en-affil= kn-affil=Uppsala University affil-num=5 en-affil= kn-affil=Uppsala University affil-num=6 en-affil= kn-affil=Uppsala University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=High Energy Accelerator Research Organization en-keyword=transition-meal compounds kn-keyword=transition-meal compounds en-keyword=electronic-structure kn-keyword=electronic-structure en-keyword=raman-scattering kn-keyword=raman-scattering en-keyword=emission-spectroscopy kn-keyword=emission-spectroscopy en-keyword=spectra kn-keyword=spectra en-keyword=absorption kn-keyword=absorption en-keyword=coo kn-keyword=coo END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=8 article-no= start-page=083511-1 end-page=083511-3 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20068 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Output properties of C60 field-effect transistor device with Eu source/drain electrodes en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Field-effect transistor (FET) device with thin films of C60 has been fabricated with Eu electrodes exhibiting small work function. The C60 FET device shows n-channel FET properties with high field-effect mobility, 0.50 cm2 V?1 s?1. Furthermore, nonvanishing drain current, i.e., normally on, is observed in this FET device. This originates from small energy barrier for electron from Eu source electrode to lowest unoccupied molecular orbital of C60.

en-copyright= kn-copyright= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OchiKenji en-aut-sei=Ochi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NaganoTakayuki en-aut-sei=Nagano en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtaToshio en-aut-sei=Ohta en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NouchiRyo en-aut-sei=Nouchi en-aut-mei=Ryo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MatsuokaYukitaka en-aut-sei=Matsuoka en-aut-mei=Yukitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShikohEiji en-aut-sei=Shikoh en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=FujiwaraAkihiko en-aut-sei=Fujiwara en-aut-mei=Akihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Japan Institute of Science and Technology affil-num=7 en-affil= kn-affil=Japan Institute of Science and Technology affil-num=8 en-affil= kn-affil=Japan Institute of Science and Technology en-keyword=device physics kn-keyword=device physics en-keyword=C60 kn-keyword=C60 en-keyword=Eu electrodes kn-keyword=Eu electrodes END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=17 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20055 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Structural differences in two polymorphs of tetra-kis-(dimethylamino)-ethylene-C-60: An x-ray diffraction study en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A type of low-temperature structure for ferromagnetic alpha-tetra-kis (dimethylamino) -ethylene (TDAE)-C-60 is proposed on the basis of low-temperature x-ray analysis. We observed that intense superlattice reflections with odd indices successively appeared below T-s = 170 K. The space group symmetry of the low-temperature phase is determined to be P2(1)/n. Two inequivalent C-60 sites exist in the low-temperature phase, which are indispensable to the orbital ordering model Of C-60. The contact configuration for the neighboring C(60)s along the stacking c direction is uniquely determined. The double bond between the hexagons faces the neighboring pentagon. We found that the surrounding TDAE molecules shift along the c axis (similar to 0.07 angstrom) and that these shifts correlate perfectly to the alignment of C-60. This result indicates that the steric effect betwee n C-60 and TDAE molecules plays an important role in the orientational ordering Of C-60, On the other hand, in the alpha' phase, no structural phase transition was observed below 30 K. This indicates that all the C(60)s are crystallographically equivalent. Structural differences separate the magnetic peculiarities of the two polymorphs in TDAE-C-60.

en-copyright= kn-copyright= en-aut-name=FujiwaraMotoyasu en-aut-sei=Fujiwara en-aut-mei=Motoyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KambeTakashi en-aut-sei=Kambe en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaKokichi en-aut-sei=Oshima en-aut-mei=Kokichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University en-keyword=Physics kn-keyword=Physics en-keyword=Condensed Matter kn-keyword=Condensed Matter END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=1 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20051 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Competing spin phases in geometrically frustrated magnetic molecules en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We identify a class of zero-dimensional classical and quantum Heisenberg spin systems exhibiting anomalous behavior in an external magnetic field B similar to that found for the geometrically frustrated kagome lattice of classical spins. Our calculations for the isotropic Heisenberg model show the emergence of a pronounced minimum in the differential susceptibility dM/dB at B-sat/3 as the temperature T is raised from 0 K for structures based on corner-sharing triangles, specifically the octahedron, cuboctahedron, and icosidodecahedron. As the first experimental evidence we note that the giant Keplerate magnetic molecule {Mo72Fe30} (Fe3+ ions on the 30 vertices of an icosidodecahedron) exhibits this behavior. For low T when Bapproximate toB(sat)/3 two competing families of spin configurations exist of which one behaves magnetically "stiff" leading to a reduction of dM/dB.

en-copyright= kn-copyright= en-aut-name=SchroderChristian en-aut-sei=Schroder en-aut-mei=Christian kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NojiriHiroyuki en-aut-sei=Nojiri en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SchnackJurgen en-aut-sei=Schnack en-aut-mei=Jurgen kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HagePeter en-aut-sei=Hage en-aut-mei=Peter kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=LubanMarshall en-aut-sei=Luban en-aut-mei=Marshall kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KogerlerPaul en-aut-sei=Kogerler en-aut-mei=Paul kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=University of Applied Sciences Bielefeld affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Universitat Osnabruck affil-num=4 en-affil= kn-affil=Universitat Osnabruck affil-num=5 en-affil= kn-affil=Iowa State University affil-num=6 en-affil= kn-affil=Iowa State University en-keyword=antiferromagnet kn-keyword=antiferromagnet en-keyword=field kn-keyword=field en-keyword=lattice kn-keyword=lattice en-keyword=spheres kn-keyword=spheres en-keyword=state kn-keyword=state END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=13 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20060407 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Close-packed structures and phase diagram of soft spheres in cylindrical pores en-subtitle= kn-subtitle= en-abstract= kn-abstract=It is shown for a model system consisting of spherical particles confined in cylindrical pores that the first ten close-packed phases are in one-to-one correspondence with the first ten ways of folding a triangular lattice, each being characterized by a roll-up vector like the single-walled carbon nanotube. Phase diagrams in pressure-diameter and temperature-diameter planes are obtained by inherent-structure calculation and molecular dynamics simulation. The phase boundaries dividing two adjacent phases are infinitely sharp in the low-temperature limit but are blurred as temperature is increased. Existence of such phase boundaries explains rich, diameter-sensitive phase behavior unique for cylindrically confined systems. en-copyright= kn-copyright= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaHideki en-aut-sei=Tanaka en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=WALLED CARBON NANOTUBES kn-keyword=WALLED CARBON NANOTUBES en-keyword=NANOCAPILLARITY kn-keyword=NANOCAPILLARITY en-keyword=MICROTUBULES kn-keyword=MICROTUBULES en-keyword=CAPILLARITY kn-keyword=CAPILLARITY en-keyword=CRYSTALS kn-keyword=CRYSTALS END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=10 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20049 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Structure and magnetic properties of the single-molecule magnet [Mn11CrO12(O2CCH3)(16)(H2O)(4)]center dot 2CH(3)COOH center dot 4H(2)O: magnetization manipulation and dipolar-biased tunneling in a Mn11Cr/Mn-12 mixed crystal en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The structural and magnetic properties of the single-molecule magnet [Mn11CrO12(O2CCH3)(16)(H2O)(4)].2CH(3)COOH.4H(2)O (Mn11Cr) are studied through the analysis of a Mn11Cr/Mn-12 (approximate to1:1) mixed crystal, where Mn-12 is [Mn12O12(O2CCH3)(16)(H2O)(4)].2CH(3)COOH.4H(2)O. X-ray absorption spectra reveal that the Cr ion in Mn11Cr is in the +3 valence state and occupies a specific Mn3+ site in the Mn-12 skeleton. High-frequency electron paramagnetic resonance (EPR) spectra are well explained by assuming that Mn11Cr is in a ground spin-state of S=19/2 with nearly the same EPR parameter set as for Mn-12. The lower spin quantum number results in lower barrier height (56.8 K) compared to Mn-12. The magnetization curves indicate a coercive field of 0.95 T for Mn11Cr at 1.8 K, nearly half that for Mn-12. Quantum tunneling of magnetization (QTM) in Mn11Cr is observed below the blocking temperature T-B, with the same field interval as for Mn-12. The magnetization of Mn11Cr and Mn-12 in the mixed crystal can be independently manipulated by utilizing the difference between their coercive fields. The resonance fields of QTM in Mn11Cr are significantly affected by the magnetization direction of Mn-12, suggesting the effect of dipolar-biased tunneling.

en-copyright= kn-copyright= en-aut-name=HachisukaHidekazu en-aut-sei=Hachisuka en-aut-mei=Hidekazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AwagaKunio en-aut-sei=Awaga en-aut-mei=Kunio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokoyamaToshihiko en-aut-sei=Yokoyama en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KuboTakeji en-aut-sei=Kubo en-aut-mei=Takeji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=GotoTakao en-aut-sei=Goto en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NojiriHiroyuki en-aut-sei=Nojiri en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Nagoya University affil-num=2 en-affil= kn-affil=Nagoya University affil-num=3 en-affil= kn-affil=Institute for Molecular Sciences affil-num=4 en-affil= kn-affil=Nara University of Education affil-num=5 en-affil= kn-affil=Graduate School of Human and Environmental Studies affil-num=6 en-affil= kn-affil=Okayama University en-keyword=high-spin molecules kn-keyword=high-spin molecules en-keyword=jahn-teller isomerism kn-keyword=jahn-teller isomerism en-keyword=ground-state kn-keyword=ground-state en-keyword=mn-12 kn-keyword=mn-12 en-keyword=complexes kn-keyword=complexes en-keyword=relaxation kn-keyword=relaxation en-keyword=susceptibility kn-keyword=susceptibility en-keyword=mn12012(02cr)(16)(H20)(4) kn-keyword=mn12012(02cr)(16)(H20)(4) en-keyword=mn12012(02CR)16(h20)4 kn-keyword=mn12012(02CR)16(h20)4 en-keyword=cluster kn-keyword=cluster en-keyword=s=9 kn-keyword=s=9 END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=23 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Metal-insulator transition at 50 K in Na2C60 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Temperature dependence of electron spin resonance in Na2C60 was studied in a temperature range from 2 to 350 K. It was shown that Na2C60 was metallic above 50 K and had a metal-insulator transition at 50 K. The center frequency for the Hg(2) Raman mode in Na2C60 at 298 K was close to those in the metallic Rb3C60, K3C60, and Cs3C60, while the linewidth was close to that in the metallic but nonsuperconducting Cs3C60. The Hg(2) mode showed a large blueshift and narrowing at 50 K. The center frequency and the linewidth in the low-temperature region from 50 K were almost the same as those in the insulating C-60 and Rb6C60, which showed the metal-insulator transition at 50 K in Na2C60. The origin of this metal-insulator transition was discussed in terms of the electron-phonon interaction (Jahn-Teller effect) and the electron-electron interaction (Mott-Hubbard picture). [S0163-1829(99)04123-5].

en-copyright= kn-copyright= en-aut-name=KubozonoY. en-aut-sei=Kubozono en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakabayashiY. en-aut-sei=Takabayashi en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujikiS. en-aut-sei=Fujiki en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KashinoS. en-aut-sei=Kashino en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KambeT. en-aut-sei=Kambe en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IwasaY. en-aut-sei=Iwasa en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=EmuraS. en-aut-sei=Emura en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=5 en-affil= kn-affil=Department of Physics, Okayama University affil-num=6 en-affil= kn-affil=Japan Advanced Institute of Science and Technology affil-num=7 en-affil= kn-affil=ISIR, Osaka University END start-ver=1.4 cd-journal=joma no-vol=63 cd-vols= no-issue=4 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20011 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Structure and physical properties of Na4C60 under ambient and high pressures en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The structure and physical properties of two-dimensional polymeric Na4C60 (body-centered monoclinic, space group I2/m) are studied in a wide temperature region from 12 to 300 K at 1 bar, and in a pressure region up to 53 kbar at 300 K. The temperature dependence of lattice constants suggests a structural anomaly below 100 K where the variation of spin susceptibility is observed from electron spin resonance. The thermal expansion of the unit-cell volume V is smaller than that of monomeric Rb3C60 and K3C60. The compressibility of c is larger than that of a and b, which can be well explained by the repulsion between Na ions. The compressibility of the center-to-center distance in the (10(1) over bar) plane is similar to1/3 times smaller that that in the (101) plane, which can be well explained by the formation of the polymer chains. Further, a possibility of a three-dimensional polymerization is discussed on the basis of the pressure dependence of C-60. . .C-60 distances.

en-copyright= kn-copyright= en-aut-name=KubozonoY. en-aut-sei=Kubozono en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakabayashiY. en-aut-sei=Takabayashi en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KambeT. en-aut-sei=Kambe en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujikiS. en-aut-sei=Fujiki en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KashinoS. en-aut-sei=Kashino en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=EmuraS. en-aut-sei=Emura en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Physics, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=5 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=6 en-affil= kn-affil=ISIR, Osaka University END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=6 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20058 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Carbon-substitution dependent multiple superconducting gap of MgB2: A sub-meV resolution photoemission study en-subtitle= kn-subtitle= en-abstract= kn-abstract=

"Sub-meV" resolution photoemission spectroscopy was used to study carbon-substitution dependence of the multiple superconducting gap of Mg(B1-xCx)(2). Two features corresponding to sigma and pi gaps are clearly observed in the raw spectra up to carbon concentration x=0.075. The observed x dependence of the two gaps shows a qualitatively different behavior: the sigma gap is proportional to T-c while the pi gap shows negligible change. Doping as well as temperature dependence can be explained within the two-band mean-field theory. Implications from the present study are discussed.

en-copyright= kn-copyright= en-aut-name=TsudaS en-aut-sei=Tsuda en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YokoyaT en-aut-sei=Yokoya en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KissT en-aut-sei=Kiss en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShimojimaT en-aut-sei=Shimojima en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShinS en-aut-sei=Shin en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TogashiT en-aut-sei=Togashi en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=WatanabeS en-aut-sei=Watanabe en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ZhangC en-aut-sei=Zhang en-aut-mei=C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ChenC T en-aut-sei=Chen en-aut-mei=C T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=LeeS en-aut-sei=Lee en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=UchiyamaH en-aut-sei=Uchiyama en-aut-mei=H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TajimaS en-aut-sei=Tajima en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=NakaiN en-aut-sei=Nakai en-aut-mei=N kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=MachidaK en-aut-sei=Machida en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=University of Tokyo affil-num=2 en-affil= kn-affil=University of Tokyo affil-num=3 en-affil= kn-affil=Institute of Physical and Chemical Research affil-num=4 en-affil= kn-affil=University of Tokyo affil-num=5 en-affil= kn-affil=University of Tokyo affil-num=6 en-affil= kn-affil=Institute of Physical and Chemical Research affil-num=7 en-affil= kn-affil=University of Tokyo affil-num=8 en-affil= kn-affil=Beijing Center for Crystal R&D affil-num=9 en-affil= kn-affil=Beijing Center for Crystal R&D affil-num=10 en-affil= kn-affil=International Superconductivity Technology Center affil-num=11 en-affil= kn-affil=International Superconductivity Technology Center affil-num=12 en-affil= kn-affil=International Superconductivity Technology Center affil-num=13 en-affil= kn-affil=Kyoto University affil-num=14 en-affil= kn-affil=Okayama University en-keyword=magnesium diboride kn-keyword=magnesium diboride en-keyword=single-crystals kn-keyword=single-crystals en-keyword=origin kn-keyword=origin en-keyword=boron kn-keyword=boron END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=2 article-no= start-page=023501 end-page=023501 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20057 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fabrication of field-effect transistor device with higher fullerene, C88 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A fullerene field-effect transistor (FET) device has been fabricated with thin films of C88, and n-channel normally-on depletion-type FET properties have been found in this FET device. The C88 FET exhibited a high mobility, μ, of 2.5 x 10-3 cm2 V-1 s-1 at 300 K, in fullerene FETs. The carrier transport showed a thermally-activated hopping transport. The n-channel normally-on FET properties and the hopping transport reflect the small mobility gap and low carrier concentration in the channel region of C88 thin-films.

en-copyright= kn-copyright= en-aut-name=NaganoTakayuki en-aut-sei=Nagano en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SugiyamaHiroyuki en-aut-sei=Sugiyama en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KuwaharaEiji en-aut-sei=Kuwahara en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WatanabeRie en-aut-sei=Watanabe en-aut-mei=Rie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KusaiHaruka en-aut-sei=Kusai en-aut-mei=Haruka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KashinoYoko en-aut-sei=Kashino en-aut-mei=Yoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=fullerene devices kn-keyword=fullerene devices en-keyword=field effect transistors kn-keyword=field effect transistors en-keyword=carrier density kn-keyword=carrier density en-keyword=carrier mobility kn-keyword=carrier mobility en-keyword=hopping conduction kn-keyword=hopping conduction END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=23 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=20026 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Structure and physical properties of Cs3+alpha C60 (alpha=0.0-1.0) under ambient and high pressures en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The intermediate phases Cs3+alphaC60 (alpha=0.0-1.0), have been prepared, and their structure and physical properties are studied by x-ray powder diffraction, Raman, ESR, electric conductivity, and ac susceptibility measurements under ambient and high pressures. The x-ray powder diffraction pattern of Cs3+alphaC60 (alpha=0.0-1.0) can be indexed as a mixture of the body-centered-orthorhombic (bco) and cubic (A15) phases. The A15 phase diminishes above 30 kbar. The broad ESR peak due to the conduction electron (c-ESR) is observed only for the phases around alpha=0.0 in Cs3+alphaC60. The resistivity of the Cs3+alphaC60 (alphanot equal0) sample follows the granular metal theory and/or Sheng model even in the sample exhibiting a broad ESR peak. No superconducting transition is observed up to 10.6 kbar in Cs3+alphaC60 (alphanot equal0). These results present that bco phase of Cs3+alphaC60 (alpha=0) is a final candidate for a pressure-induced superconductor.

en-copyright= kn-copyright= en-aut-name=FujikiS. en-aut-sei=Fujiki en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KubozonoY. en-aut-sei=Kubozono en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiM. en-aut-sei=Kobayashi en-aut-mei=M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KambeT. en-aut-sei=Kambe en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=RikiishiY. en-aut-sei=Rikiishi en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KashinoS. en-aut-sei=Kashino en-aut-mei=S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IshiiK. en-aut-sei=Ishii en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SuematsuH. en-aut-sei=Suematsu en-aut-mei=H. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=FujiwaraA. en-aut-sei=Fujiwara en-aut-mei=A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Vacuum UV Photoscience, Institute for Molecular Science affil-num=3 en-affil= kn-affil=Department of Materials Science, Himeji Institute of Technology affil-num=4 en-affil= kn-affil=Department of Physics, Okayama University affil-num=5 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=6 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=7 en-affil= kn-affil=Department of Physics, The University of Tokyo affil-num=8 en-affil= kn-affil=Department of Physics, The University of Tokyo affil-num=9 en-affil= kn-affil=Japan Advanced Institute of Science and Technology END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=14 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fabrication of C60 field-effect transistors with polyimide and Ba0.4Sr0.6Ti0.96O3 gate insulators en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Flexible C60 field-effect transistor (FET) device has been fabricated with polyimide gate insulator on the poly(ethylene terephthalate) substrate, and n-channel normally-off FET properties are observed in this FET device. The field-effect mobility, ?, is estimated to be ~10-2 cm2 V-1 s-1 at 300 K. Furthermore, the C60 FET has been fabricated with high dielectric Ba0.4Sr0.6Ti0.96O3 (BST) gate insulator, showing n-channel properties; the ? value is estimated to be ~10-4 cm2 V-1 s-1 at 300 K. The FET device operates at very low gate voltage, VG, and low drain-source voltage, VDS. Thus these C60 FET devices possess flexibility and low-voltage operation characteristic of polyimide and BST gate insulators, respectively.

en-copyright= kn-copyright= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NaganoTakayuki en-aut-sei=Nagano en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HaruyamaYusuke en-aut-sei=Haruyama en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KuwaharaEiji en-aut-sei=Kuwahara en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OchiKenji en-aut-sei=Ochi en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=FujiwaraAkihiko en-aut-sei=Fujiwara en-aut-mei=Akihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Japan Advanced Institute of Science and Technology en-keyword=fullerene devices kn-keyword=fullerene devices en-keyword=insulated gate field effect transistors kn-keyword=insulated gate field effect transistors en-keyword=polymers kn-keyword=polymers en-keyword=barium compounds kn-keyword=barium compounds en-keyword=strontium compounds kn-keyword=strontium compounds en-keyword=dielectric materials kn-keyword=dielectric materials END start-ver=1.4 cd-journal=joma no-vol=122 cd-vols= no-issue=10 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Phase diagram of water between hydrophobic surfaces en-subtitle= kn-subtitle= en-abstract= kn-abstract=Molecular dynamics simulations demonstrate that there are at least two classes of quasi-two-dimensional solid water into which liquid water confined between hydrophobic surfaces freezes spontaneously and whose hydrogen-bond networks are as fully connected as those of bulk ice. One of them is the monolayer ice and the other is the bilayer solid which takes either a crystalline or an amorphous form. Here we present the phase transformations among liquid, bilayer amorphous (or crystalline) ice, and monolayer ice phases at various thermodynamic conditions, then determine curves of melting, freezing, and solid-solid structural change on the isostress planes where temperature and intersurface distance are variable, and finally we propose a phase diagram of the confined water in the temperature-pressure-distance space. en-copyright= kn-copyright= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaHideki en-aut-sei=Tanaka en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University en-keyword=MOLECULAR-DYNAMICS SIMULATION kn-keyword=MOLECULAR-DYNAMICS SIMULATION en-keyword=CONFINED WATER kn-keyword=CONFINED WATER en-keyword=LIQUID WATER kn-keyword=LIQUID WATER en-keyword=SOLVATION FORCES; CARBON NANOTUBES kn-keyword=SOLVATION FORCES; CARBON NANOTUBES en-keyword=BILAYER ICE kn-keyword=BILAYER ICE en-keyword=EQUILIBRIA kn-keyword=EQUILIBRIA en-keyword=TRANSITION kn-keyword=TRANSITION en-keyword=WALLS kn-keyword=WALLS en-keyword=INTERFACE kn-keyword=INTERFACE END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=8 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070828 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Phase equilibria and interfacial tension of fluids confined in narrow pores en-subtitle= kn-subtitle= en-abstract= kn-abstract=Correlation between phase behaviors of a Lennard-Jones fluid in and outside a pore is examined over wide thermodynamic conditions by grand canonical Monte Carlo simulations. A pressure tensor component of the confined fluid, a variable controllable in simulation but usually uncontrollable in experiment, is related with the pressure of a bulk homogeneous system in equilibrium with the confined system. Effects of the pore dimensionality, size, and attractive potential on the correlations between thermodynamic properties of the confined and bulk systems are clarified. A fluid-wall interfacial tension defined as an excess grand potential is evaluated as a function of the pore size. It is found that the tension decreases linearly with the inverse of the pore diameter or width. en-copyright= kn-copyright= en-aut-name=HamadaYoshinobu en-aut-sei=Hamada en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaHideki en-aut-sei=Tanaka en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=MONTE-CARLO-SIMULATION kn-keyword=MONTE-CARLO-SIMULATION en-keyword=CARBON NANOTUBES kn-keyword=CARBON NANOTUBES en-keyword=WATER kn-keyword=WATER en-keyword=TRANSITION kn-keyword=TRANSITION en-keyword=NANOSPACES kn-keyword=NANOSPACES en-keyword=ADSORPTION kn-keyword=ADSORPTION en-keyword=NANOPORES kn-keyword=NANOPORES en-keyword=SURFACE kn-keyword=SURFACE en-keyword=LIQUID kn-keyword=LIQUID en-keyword=WALLS kn-keyword=WALLS END start-ver=1.4 cd-journal=joma no-vol=69 cd-vols= no-issue=4 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20037 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Scanning tunneling microscopy of Dy@C82 and Dy@C60 adsorbed on Si(111)-(7x7) surfaces en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Dy@C-82 and Dy@C-60 adsorbed on Si(111)-(7x7) surface are investigated by scanning tunneling microscopy (STM) at 295 K. The Dy@C-82 molecules in the first layer are adsorbed on the Si(111)-(7x7) surface without formation of islands and nucleation, and the internal structure of the Dy@C-82 molecule is first observed on the surface at 295 K. The average heights of the Dy@C-82 molecules in the first and second layers are estimated to be 7.2 and 10.8 A, respectively, by STM. These results suggest strong interactions between the Si atoms and the Dy@C-82 molecules in the first layer. The STM image reveals that the Dy@C-60 molecule is nearly spherical, showing that the metal endohedral C-60 possesses a cage-form structure.

en-copyright= kn-copyright= en-aut-name=FujikiSatoshi en-aut-sei=Fujiki en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HosokawaTomoko en-aut-sei=Hosokawa en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KanbaraTakayoshi en-aut-sei=Kanbara en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FujiwaraAkihiko en-aut-sei=Fujiwara en-aut-mei=Akihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NonogakiYouichi en-aut-sei=Nonogaki en-aut-mei=Youichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=UrisuTsuneo en-aut-sei=Urisu en-aut-mei=Tsuneo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Graduate University for Advanced Studies affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Tohoku University affil-num=5 en-affil= kn-affil=Japan Advanced Institute of Science and Technology affil-num=6 en-affil= kn-affil=Institute for Molecular Science affil-num=7 en-affil= kn-affil=Graduate University for Advanced Studies en-keyword=endohedral metallofullerenes kn-keyword=endohedral metallofullerenes en-keyword=electronic-properties kn-keyword=electronic-properties en-keyword=c-60 kn-keyword=c-60 END start-ver=1.4 cd-journal=joma no-vol=329-333 cd-vols= no-issue= article-no= start-page=713 end-page=714 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20035 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=X-ray study of modulated structures of beta-CuxV2O5 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

High resolution X-ray study reveals the wave vector change in the modulated structure of the quasi-one dimensional compound beta'-Cu vanadium bronze. Structural modulation of the reduced wave vector q0 = (0, 0.305, 0) emerges below 220 K in beta'-Cu0.29V2O5 . For beta'-Cu0.39V2O5, not the single q modulation but two kinds of modulations were observed. A three-fold superlattice structure with q1 = (0, 0.333, 0) appears below 210 K. An incommensurate modulated structure with q2 = (0, 0.26 ? 0.29, 0) coexists below 175 K, whose satellite intensity and b* component Qb have temperature and passing-time dependencies between 140 K and 175 K. The competition between q1 and q2 modulations was also observed. It seems that the q2 is deeply related to the physical property change between 140 K and 180 K confirmed by the decrease in the magnetic susceptibility and the increase in the resistivity.

en-copyright= kn-copyright= en-aut-name=NagaoNobuaki en-aut-sei=Nagao en-aut-mei=Nobuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NogamiYoshio en-aut-sei=Nogami en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaKokichi en-aut-sei=Oshima en-aut-mei=Kokichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamadaHiroyuki en-aut-sei=Yamada en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=UedaYutaka en-aut-sei=Ueda en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=University of Tokyo affil-num=5 en-affil= kn-affil=University of Tokyo en-keyword=x-ray kn-keyword=x-ray en-keyword=modulated structures kn-keyword=modulated structures en-keyword=low dimensional conductor kn-keyword=low dimensional conductor en-keyword=vanadium bronze kn-keyword=vanadium bronze en-keyword=b-cuxv2o5 kn-keyword=b-cuxv2o5 END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=17 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200710 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antiferromagnetic ordering driven by the molecular orbital order of C-60 in alpha '-tetra-kis-(dimethylamino)-ethylene-C-60 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We have studied the ground state of a fullerene-based magnet, the alpha'-phase tetra-kis-(dimethylamino)ethylene-C-60 (alpha'-TDAE-C-60), by electron spin resonance and magnetic torque measurements. Below T-N = 7 K, nonparamagnetic field dependent resonances with a finite excitation gap ( 1.7 GHz) are observed along the a axis. Strong enhancement in their intensity as temperature is decreased is inconsistent with excitation from a singlet state, which had been proposed for the alpha'-phase ground state. Below T-N, nonquadratic field dependence of the magnetic torque signal is also observed in contrast to quadratic field dependence in the paramagnetic phase. The angle-dependent torque signals below T-N indicate the existence of an anisotropy of the bulk magnetization. From both experiments, we propose an antiferro-magnetic ground state driven by the cooperative orientational ordering of C-60 in the alpha'-TDAE-C-60.

en-copyright= kn-copyright= en-aut-name=KambeTakashi en-aut-sei=Kambe en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KajiyoshiKoichi en-aut-sei=Kajiyoshi en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiwaraMotoyasu en-aut-sei=Fujiwara en-aut-mei=Motoyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OshimaKokichi en-aut-sei=Oshima en-aut-mei=Kokichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University en-keyword=TDAE-C-60 kn-keyword=TDAE-C-60 en-keyword=Ferromagnetism kn-keyword=Ferromagnetism en-keyword=(NH3)K3C60 kn-keyword=(NH3)K3C60 en-keyword=ESR kn-keyword=ESR END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=22 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20056 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Superconductivity of the ternary boride Li2Pd3B probed by B-11 NMR en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We report a B-11 NMR measurement on the recently discovered superconductor Li2Pd3B. The nuclear spin lattice relaxation rate 1/T-1 shows a well-defined coherence peak just below T-c(H=1.46 T)=5.7 K, and the spin susceptibility measured by the Knight shift also decreases below T-c. These results indicate that the superconductivity is of conventional nature, with an isotropic gap. Our results also suggest that the p-electrons of boron and the d-electrons of palladium that hybridize with boron p-electrons are primarily responsible for the superconductivity.

en-copyright= kn-copyright= en-aut-name=NishiyamaM en-aut-sei=Nishiyama en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InadaY en-aut-sei=Inada en-aut-mei=Y kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ZhengGuo-qing en-aut-sei=Zheng en-aut-mei=Guo-qing kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=5 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20062 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Field-induced ferromagnetic order and colossal magnetoresistance in la1.2Sr1.8mn2O7:a La139 nmr study en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In order to gain insights into the origin of colossal magnetoresistance (CMR) in manganese oxides, we performed a La-139 NMR study in the double-layered compound La1.2Sr1.8Mn2O7. We find that above the Curie temperature T-C = 126 K, applying a magnetic field induces a long-range ferromagnetic order that persists up to T = 330 K. The critical field at which the induced magnetic moment is saturated coincides with the field at which the CMR effect reaches a maximum. Our results therefore indicate that the CMR observed above T-C in this compound is due to the field-induced ferromagnetism that produces a metallic state via the double exchange interaction.

en-copyright= kn-copyright= en-aut-name=ShiotaniY en-aut-sei=Shiotani en-aut-mei=Y kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SarraoJ L en-aut-sei=Sarrao en-aut-mei=J L kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ZhengGuo-qing en-aut-sei=Zheng en-aut-mei=Guo-qing kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Osaka University affil-num=2 en-affil= kn-affil=Los Alamos National Laboratory affil-num=3 en-affil= kn-affil=Okayama University en-keyword=equal-to 0.50 kn-keyword=equal-to 0.50 en-keyword=giant magnetoresistance kn-keyword=giant magnetoresistance en-keyword=resistivity kn-keyword=resistivity en-keyword=manganites kn-keyword=manganites en-keyword=phase kn-keyword=phase en-keyword=crystal kn-keyword=crystal en-keyword=oxides kn-keyword=oxides END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=7 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20052 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Two-photon spectroscopy of core excitons in barium fluoride using synchrotron radiation and laser light en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We have conducted two-photon spectroscopy of core excitons in BaF2. Synchrotron radiation and laser light were used for 5p core-electron excitation and Auger-free luminescence was detected as the signal. Two-photon excitation enables access to f and p orbitals that cannot be reached by one-photon excitation of electrons in p orbitals. It has been found that the spin-orbit splittings of 4f and 6p states of the Ba ion in BaF2 are 0.7 +/- 0.1 and 1.4 +/- 0.1 eV, respectively.

en-copyright= kn-copyright= en-aut-name=TsujibayashiToru en-aut-sei=Tsujibayashi en-aut-mei=Toru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ItohMinoru en-aut-sei=Itoh en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AzumaJunpei en-aut-sei=Azuma en-aut-mei=Junpei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WatanabeMasayuki en-aut-sei=Watanabe en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ArimotoOsamu en-aut-sei=Arimoto en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NakanishiShunsuke en-aut-sei=Nakanishi en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Osaka Dental University affil-num=2 en-affil= kn-affil=Shinshu University affil-num=3 en-affil= kn-affil=Synchrotron Light Application Center affil-num=4 en-affil= kn-affil=Kyoto University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Kagawa University en-keyword=electronicstructure kn-keyword=electronicstructure en-keyword=spectra kn-keyword=spectra en-keyword=absorption kn-keyword=absorption en-keyword=crystals kn-keyword=crystals en-keyword=baf2 kn-keyword=baf2 en-keyword=sns kn-keyword=sns en-keyword=ges kn-keyword=ges END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=23 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19972 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Scanning tunneling microscopy/spectroscopy studies of two isomers of Ce@C82 on Si(111)-(7×7）surfaces en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Scanning tunneling microscopy images for two isomers of Ce@C-82 were observed on Si(111)-(7x7) at 295 K. The Ce@C-82 molecules in the first layer were bound to the Si surfaces, and the motions were frozen even at 295 K. The multilayer of the Ce@C-82 isomer I (Ce@C-82-I) produced a close-packed structure in the surface layer by annealing the Si substrate at 473 K. The distance between the nearest-neighboring molecules was 1.15(4) nm whose value was consistent with that, 1.12 nm, estimated from x-ray diffraction of the Ce@C-82-I crystals. This implies that the close-packed structure is dominated by van der Waals forces, as in crystals of Ce@C-82-I. The internal structure of Ce@C-82-I was observed in the first layer due to a freeze of molecular motion caused by strong interactions between the molecule and the Si adatoms in the surface. Scanning tunneling spectroscopy revealed that the energy gaps for Ce@C-82-I and -II in the first layer opened to gap energies, E-g of 0.7 and 1.0 eV, respectively. This fact suggests that these molecules are semiconductors with smaller value of E-g than those for C-60 and C-70.

en-copyright= kn-copyright= en-aut-name=FujikiSatoshi en-aut-sei=Fujiki en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=RikiishiYoshie en-aut-sei=Rikiishi en-aut-mei=Yoshie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UrisuTsuneo en-aut-sei=Urisu en-aut-mei=Tsuneo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Graduate University for Advanced Studies affil-num=2 en-affil= kn-affil=Science and Technology Agency affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Graduate University for Advanced Studies en-keyword=electronic-structure kn-keyword=electronic-structure en-keyword=endohedral metallofullerenes kn-keyword=endohedral metallofullerenes en-keyword=microscopy kn-keyword=microscopy en-keyword=lanthanum kn-keyword=lanthanum en-keyword=crystal kn-keyword=crystal en-keyword=anion kn-keyword=anion en-keyword=films kn-keyword=films END start-ver=1.4 cd-journal=joma no-vol=121 cd-vols= no-issue=15 article-no= start-page=7304 end-page=7312 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20041015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hydrophobic effect in the pressure-temperature plane en-subtitle= kn-subtitle= en-abstract= kn-abstract=The free energy of the hydrophobic hydration and the strength of the solvent-mediated attraction between hydrophobic solute molecules are calculated in the pressure-temperature plane. This is done in the framework of an exactly soluble model that is an extension of the lattice model proposed by Kolomeisky and Widom [A. B. Kolomeisky and B. Widom, Faraday Discuss. 112, 81 (1999)]. The model takes into account both the mechanism of the hydrophobic effect dominant at low temperatures and the opposite mechanism of solvation appearing at high temperatures and has the pressure as a second thermodynamic variable. With this model, two boundaries are identified in the pressure-temperature plane: the first one within which the solubility, or the Ostwald absorption coefficient, decreases with increasing temperature at fixed pressure and the second one within which the strength of solvent-mediated attraction increases with increasing temperature. The two are nearly linear and parallel to each other, and the second boundary lies in the low-temperature and low-pressure side of the first boundary. It is found that a single, near-linear relation between the hydration free energy and the strength of the hydrophobic attraction holds over the entire area within the second boundary in the pressure-temperature plane. (C) 2004 American Institute of Physics. en-copyright= kn-copyright= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=FREE-ENERGY kn-keyword=FREE-ENERGY en-keyword=AQUEOUS ARGON kn-keyword=AQUEOUS ARGON en-keyword=DEPENDENCE kn-keyword=DEPENDENCE en-keyword=WATER kn-keyword=WATER en-keyword=HYDRATION kn-keyword=HYDRATION en-keyword=ENTROPY kn-keyword=ENTROPY en-keyword=MODEL kn-keyword=MODEL en-keyword=DENATURATION kn-keyword=DENATURATION en-keyword=SIMULATIONS kn-keyword=SIMULATIONS en-keyword=ATTRACTION kn-keyword=ATTRACTION END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=14 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20064 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Tomonaga-luttinger liquid in a quasi-one-dimensional s=1 antiferromagnet observed by specific heat measurements en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Specific-heat experiments on single crystals of the S=1 quasi-one-dimensional bond-alternating antiferromagnet Ni(C9H24N4)(NO2)ClO4 (NTENP) have been performed in magnetic fields applied both parallel and perpendicular to the spin chains. We have found for the parallel field configuration that the magnetic specific heat (C-mag) is proportional to temperature (T) above a critical field H-c, at which the energy gap vanishes, in a temperature region above that of the long-range ordered state. The ratio C-mag/T increases as the magnetic field approaches H-c from above. The data are in good quantitative agreement with the prediction of the c=1 conformal field theory in conjunction with the velocity of the excitations calculated by a numerical diagonalization, providing conclusive evidence for a Tomonaga-Luttinger liquid.

en-copyright= kn-copyright= en-aut-name=HagiwaraM en-aut-sei=Hagiwara en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsujiiH en-aut-sei=Tsujii en-aut-mei=H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=RotunduC R en-aut-sei=Rotundu en-aut-mei=C R kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AndrakaB en-aut-sei=Andraka en-aut-mei=B kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakanoY en-aut-sei=Takano en-aut-mei=Y kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TateiwaN en-aut-sei=Tateiwa en-aut-mei=N kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KobayashiT C en-aut-sei=Kobayashi en-aut-mei=T C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SuzukiT en-aut-sei=Suzuki en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SugaS en-aut-sei=Suga en-aut-mei=S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Osaka University affil-num=2 en-affil= kn-affil=The Institute of Physical and Chemical Research affil-num=3 en-affil= kn-affil=University of Florida affil-num=4 en-affil= kn-affil=University of Florida affil-num=5 en-affil= kn-affil=University of Florida affil-num=6 en-affil= kn-affil=Japan Atomic Energy Agency affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Osaka University affil-num=9 en-affil= kn-affil=Osaka University en-keyword=magnetic-field kn-keyword=magnetic-field en-keyword=alternating chain kn-keyword=alternating chain en-keyword=heisenberg chain kn-keyword=heisenberg chain en-keyword=phasetransition kn-keyword=phasetransition en-keyword=cu-2 (c5h12n2)(2)cl-4 kn-keyword=cu-2 (c5h12n2)(2)cl-4 en-keyword=ladders kn-keyword=ladders en-keyword=condensation kn-keyword=condensation en-keyword=charge kn-keyword=charge en-keyword=f5pnn kn-keyword=f5pnn END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=12 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20069 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Generic phase diagram of fermion superfluids with population imbalance en-subtitle= kn-subtitle= en-abstract= kn-abstract=

It is shown by microscopic calculations for trapped imbalanced Fermi superfluids that the gap function always has sign changes, i.e., the Fulde-Ferrell-Larkin-Ovchinnikov (FFLO)-like state, up to a critical imbalance P-c, beyond which normal state becomes stable, at temperature T=0. A temperature-versus-pressure phase diagram is constructed, where the BCS state without sign change is stable only at T not equal 0. We reproduce the observed bimodality in the density profile to identify its origin and evaluate P-c as functions of T and the coupling strength. These dependencies match with the recent experiments.

en-copyright= kn-copyright= en-aut-name=MachidaK en-aut-sei=Machida en-aut-mei=K kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MizushimaT en-aut-sei=Mizushima en-aut-mei=T kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IchiokaM en-aut-sei=Ichioka en-aut-mei=M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=molecular-field kn-keyword=molecular-field en-keyword=superconductivity kn-keyword=superconductivity en-keyword=gas kn-keyword=gas END start-ver=1.4 cd-journal=joma no-vol=409 cd-vols= no-issue=4-6 article-no= start-page=187 end-page=191 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fabrication and characterization of field-effect transistor device with C2v isomer of Pr@C82 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A field-effect transistor (FET) device was fabricated with thin films of C2v isomer of Pr@C82. This device apparently showed n-channel normally-on type FET properties, where non-zero current was observed at gate-source voltage of 0 VGS, of 0V. Normally off FET properties were observed by subtraction of the non-zero current from the drain current.Thus the normally on properties are ascribed to the high bulk current caused by the small energy gap ≈0.3 eV. The field-effect mobility for this FET was 1.5 x 10-4 cm2 V-1 s-1 at 320 K, being comparable to those of other endohedral metallofullerene FET devices.

en-copyright= kn-copyright= en-aut-name=NaganoTakayuki en-aut-sei=Nagano en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KuwaharaEiji en-aut-sei=Kuwahara en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FujiwaraAkihiko en-aut-sei=Fujiwara en-aut-mei=Akihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=5 en-affil= kn-affil=CREST, Japan Science and Technology Agency en-keyword=Field effect transistors kn-keyword=Field effect transistors END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=4 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070728 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On the thermodynamic stability of hydrogen clathrate hydrates en-subtitle= kn-subtitle= en-abstract= kn-abstract=The cage occupancy of hydrogen clathrate hydrate has been examined by grand canonical Monte Carlo (GCMC) simulations for wide ranges of temperature and pressure. The simulations are carried out with a fixed number of water molecules and a fixed chemical potential of the guest species so that hydrogen molecules can be created or annihilated in the clathrate. Two types of the GCMC simulations are performed; in one the volume of the clathrate is fixed and in the other it is allowed to adjust itself under a preset pressure so as to take account of compression by a hydrostatic pressure and expansion due to multiple cage occupancy. It is found that the smaller cage in structure II is practically incapable of accommodating more than a single guest molecule even at pressures as high as 500 MPa, which agrees with the recent experimental investigations. The larger cage is found to encapsulate at most 4 hydrogen molecules, but its occupancy is dependent significantly on the pressure of hydrogen. en-copyright= kn-copyright= en-aut-name=KatsumasaKeisuke en-aut-sei=Katsumasa en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaHideki en-aut-sei=Tanaka en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=OCCUPANCY kn-keyword=OCCUPANCY en-keyword=CLUSTERS kn-keyword=CLUSTERS en-keyword=STORAGE kn-keyword=STORAGE en-keyword=CAGES kn-keyword=CAGES en-keyword=WATER kn-keyword=WATER END start-ver=1.4 cd-journal=joma no-vol=122 cd-vols= no-issue=7 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050215 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On the thermodynamic stability and structural transition of clathrate hydrates en-subtitle= kn-subtitle= en-abstract= kn-abstract=Gas mixtures of methane and ethane form structure II clathrate hydrates despite the fact that each of pure methane and pure ethane gases forms the structure I hydrate. Optimization of the interaction potential parameters for methane and ethane is attempted so as to reproduce the dissociation pressures of each simple hydrate containing either methane or ethane alone. An account for the structural transitions between type I and type II hydrates upon changing the mole fraction of the gas mixture is given on the basis of the van der Waals and Platteeuw theory with these optimized potentials. Cage occupancies of the two kinds of hydrates are also calculated as functions of the mole fraction at the dissociation pressure and at a fixed pressure well above the dissociation pressure. en-copyright= kn-copyright= en-aut-name=KoyamaYuji en-aut-sei=Koyama en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaHideki en-aut-sei=Tanaka en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=STRUCTURE-II kn-keyword=STRUCTURE-II en-keyword=POTENTIAL FUNCTIONS kn-keyword=POTENTIAL FUNCTIONS en-keyword=ETHANE kn-keyword=ETHANE en-keyword=METHANE kn-keyword=METHANE en-keyword=GAS kn-keyword=GAS en-keyword=MOLECULES kn-keyword=MOLECULES en-keyword=MIXTURES kn-keyword=MIXTURES en-keyword=PROPANE kn-keyword=PROPANE en-keyword=WATER kn-keyword=WATER END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=10 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20063 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Observation of a half step magnetization in the {Cu-3}-type triangular spin ring en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We report pulsed field magnetization and ESR experiments on a {Cu-3} nanomagnet, where antiferromagnetically coupled Cu2+ (S=1/2) ions form a slightly distorted triangle. The remarkable feature is the observation of a half step magnetization, hysteresis loops, and an asymmetric magnetization between a positive and a negative field in a fast sweeping external field. This is attributed to an adiabatic change of magnetization. The energy levels determined by ESR unveil that the different mixing nature of a spin chirality of a total S=1/2 Kramers doublet by virtue of Dzyaloshinskii-Moriya interactions is decisive for inducing half step magnetization.

en-copyright= kn-copyright= en-aut-name=ChoiKwang-Yong en-aut-sei=Choi en-aut-mei=Kwang-Yong kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsudaYasuhiro H en-aut-sei=Matsuda en-aut-mei=Yasuhiro H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NojiriHiroyuki en-aut-sei=Nojiri en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Tohoku University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Tohoku University en-keyword=molecular magnets kn-keyword=molecular magnets en-keyword=quantum dynamics kn-keyword=quantum dynamics END start-ver=1.4 cd-journal=joma no-vol=68 cd-vols= no-issue=14 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200310 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Polymer phase of the tetrakis(dimethylamino)ethylene-C-60 organic ferromagnet en-subtitle= kn-subtitle= en-abstract= kn-abstract=

High-pressure electron spin resonance (ESR) measurements were performed on tetrakis(dimethylamino) ethylene (TDAE)-C-60 single crystals and stability of the polymeric phase was established in the P-T parameter space. At 7 kbar the system undergoes a ferromagnetic to paramagnetic phase transition due to the pressure-induced polymerization. The polymeric phase remains stable after the pressure release. The depolymerization of the pressure-induced phase was observed at a temperature of 520 K, revealing an unexpectedly high thermal stability of the polymer. Below room temperature, the polymeric phase behaves as a simple Curie-type insulator with one unpaired electron spin per chemical formula. The TDAE(+) donor-related unpaired electron spins, formerly ESR silent, become active above a temperature of 320 K, which demonstrates that the magnetic properties are profoundly defined by miniscule reorientation of TDAE molecules.

en-copyright= kn-copyright= en-aut-name=GarajSlaven en-aut-sei=Garaj en-aut-mei=Slaven kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KambeTakashi en-aut-sei=Kambe en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ForróLászló en-aut-sei=Forró en-aut-mei=László kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SienkiewiczAndrzej en-aut-sei=Sienkiewicz en-aut-mei=Andrzej kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FujiwaraMotoyasu en-aut-sei=Fujiwara en-aut-mei=Motoyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OshimaKokichi en-aut-sei=Oshima en-aut-mei=Kokichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Institute of Physics of Complex Matter, École Polytechnique Fédérale de Lausanne affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Institute of Physics of Complex Matter, École Polytechnique Fédérale de Lausanne affil-num=4 en-affil= kn-affil=Institute of Physics, Polish Academy of Sciences affil-num=5 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University END start-ver=1.4 cd-journal=joma no-vol=121 cd-vols= no-issue=11 article-no= start-page=5488 end-page=5493 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20040915 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On the thermodynamic stability of clathrate hydrates IV: Double occupancy of cages en-subtitle= kn-subtitle= en-abstract= kn-abstract=We have extended the van der Waals and Platteeuw theory to treat multiple occupancy of a single cage of clathrate hydrates, which has not been taken into account in the original theory but has been experimentally confirmed as a real entity. We propose a simple way to calculate the free energy of multiple cage occupancy and apply it to argon clathrate structure II in which a larger cage can be occupied by two argon atoms. The chemical potential of argon is calculated treating it as an imperfect gas, which is crucial to predict accurate pressure dependence of double occupancy expected at high pressure. It is found that double occupancy dominates over single occupancy when the guest pressure in equilibrium with the clathrate hydrate exceeds 270 MPa. (C) 2004 American Institute of Physics. en-copyright= kn-copyright= en-aut-name=TanakaHideki en-aut-sei=Tanaka en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakatsukaTakeharu en-aut-sei=Nakatsuka en-aut-mei=Takeharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KogaKenichiro en-aut-sei=Koga en-aut-mei=Kenichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=RAMAN-SCATTERING kn-keyword=RAMAN-SCATTERING en-keyword=HIGH-PRESSURES kn-keyword=HIGH-PRESSURES en-keyword=LIQUID WATER kn-keyword=LIQUID WATER en-keyword=AR HYDRATE kn-keyword=AR HYDRATE en-keyword=MOLECULES kn-keyword=MOLECULES END start-ver=1.4 cd-journal=joma no-vol=43 cd-vols= no-issue=2 article-no= start-page=125 end-page=131 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Neutrophil and lymphocyte responses to oral Streptococcus in Adamantiades-Behcet's disease en-subtitle= kn-subtitle= en-abstract= kn-abstract=Immune reactions against microorganisms play an important pathogenic role in Adamantiades-Behçet’s disease (ABD). We had previously obtained Streptococcus sanguinis (strain BD113-20) isolated from the oral cavity of patients with ABD. To investigate the pathogenesis of this isolate, we examined neutrophil 5 reactions and level of cytokine production by lymphocytes after stimulation with the strain. The reactions of neutrophils were examined by chemiluminescence assay using whole blood. The amounts of interferon gamma (IFN-g) and interleukin (IL)-4, IL-8, IL-10, and IL-12 produced by peripheral blood mononuclear cells (PBMCs) were measured by ELISA. 10 Strain BD113-20 activated neutrophils from patients with ABD and healthy volunteers, and, in addition it increased IFN-g production by lymphocytes. Lymphocyte from the patients with ABD showed a dominant T helper 1 (Th-1) immune response. Results indicated that both bacterial stimulation and host hypersensitivity might be involved in the symptoms and pathogenesis of ABD. en-copyright= kn-copyright= en-aut-name=KurauchiTomomi en-aut-sei=Kurauchi en-aut-mei=Tomomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YokotaKenji en-aut-sei=Yokota en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsuoToshihiko en-aut-sei=Matsuo en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujinamiYoshihito en-aut-sei=Fujinami en-aut-mei=Yoshihito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IsogaiEmiko en-aut-sei=Isogai en-aut-mei=Emiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IsogaiHiroshi en-aut-sei=Isogai en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OhtsukiHiroshi en-aut-sei=Ohtsuki en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OgumaKeiji en-aut-sei=Oguma en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=2 en-affil= kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry affil-num=3 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=4 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=5 en-affil= kn-affil=Department of Preventive Dentistry, Health Sciences University of Hokkaido affil-num=6 en-affil= kn-affil=Division of Animal Experimentation, Sapporo Medical University affil-num=7 en-affil= kn-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry affil-num=8 en-affil= kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry en-keyword=Adamantiades-Behcet's disease kn-keyword=Adamantiades-Behcet's disease en-keyword=Streptococcus sanguinis kn-keyword=Streptococcus sanguinis en-keyword=neutrophil kn-keyword=neutrophil en-keyword=chemiluminescence kn-keyword=chemiluminescence en-keyword=IL-8 kn-keyword=IL-8 en-keyword=T helper-1 kn-keyword=T helper-1 en-keyword=IFN-gamma kn-keyword=IFN-gamma en-keyword=IL-12 kn-keyword=IL-12 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=1 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080314 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Modelling the dynamics and control of Schistosoma japonicum transmission on Bohol island, the Philippines en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We have investigated a mathematical model for the transmission of Schistosoma japonicum in the infested region of northeastern Bohol island in the Philippines. The development of transmission models is important for planning control strategies. Since S. japonicum has a complicated mode of transmission, the rates of transmission among its hosts cannot be measured directly by field observation. Instead, they have been estimated through model analysis. The model takes into account the seasonal variations and includes a function of control measures. In 1981, a project to eliminate schistosomiasis started on Bohol island. The prevalence decreased dramatically and has kept low level less than 1%. The simulations based on the model predicted that there is little probability of resurgence of an epidemic in the northeastem endemic villages of Bohol island due to the fact that the project has attained a high coverage of selective mass treatment based oil stool examination accompanied by a successful snail control operation. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

en-copyright= kn-copyright= en-aut-name=IshikawaHirofumi en-aut-sei=Ishikawa en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhmaeHiroshi en-aut-sei=Ohmae en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=PangilinanRogelio en-aut-sei=Pangilinan en-aut-mei=Rogelio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=RedullaApolinario en-aut-sei=Redulla en-aut-mei=Apolinario kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsudaHajime en-aut-sei=Matsuda en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=National Institute of Infectious Diseases affil-num=3 en-affil= kn-affil=Schistosomiasis Control Team affil-num=4 en-affil= kn-affil=Schistosomiasis Control Team affil-num=5 en-affil= kn-affil=Dokkyo University School of Medicine en-keyword=schistosomiasis japonica kn-keyword=schistosomiasis japonica en-keyword=control kn-keyword=control en-keyword=mathematical model kn-keyword=mathematical model en-keyword=Philippines kn-keyword=Philippines en-keyword=Bohol kn-keyword=Bohol END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=3 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The evaluation of control measures against Schistosoma mekongi in Cambodia by a mathematical model en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We constructed a mathematical model for the transmission of Schistosoma mekongi in Cambodia. The simulation of the model will be instrumental in planning schistosomiasis control measures. The model includes two definitive hosts, humans and dogs, as animal reservoirs. Dogs are recognized to play an important role in schistosomiasis transmission in Cambodia. For the purpose of dealing with age-specific prevalence and intensity of infection, the human population was classified into eight age categories in the model. To describe the seasonal fluctuation of the intermediate host population of S. mekongi, the "Post-Spate Survival" hypothesis was adopted for the population dynamics of Neotricula aperta present in the Mekong River. We carried out simulations to evaluate the effect of universal treatment (UT) and targeted mass treatment (TT) with praziquantel on the reduction in prevalence of S. mekongi. The simulations indicated that biyearly UT for 8 years or yearly TT for 5 years after three courses of yearly UT could reduce the prevalence to below 5% when a UT or TT coverage of 85% of inhabitants was achieved. The simulation suggested that the suppression of S. mekongi in Cambodia would be possible by UT or TT with a high coverage rate.

en-copyright= kn-copyright= en-aut-name=HisakaneNaoto en-aut-sei=Hisakane en-aut-mei=Naoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KirinokiMasashi en-aut-sei=Kirinoki en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ChigusaYuichi en-aut-sei=Chigusa en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SinuonMuth en-aut-sei=Sinuon en-aut-mei=Muth kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SocheatDuong en-aut-sei=Socheat en-aut-mei=Duong kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MatsudaHajime en-aut-sei=Matsuda en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IshikawaHirofumi en-aut-sei=Ishikawa en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Human Ecology, Graduate School of Environmental Science, Okayama University affil-num=2 en-affil= kn-affil=Center for Tropical Medicine and Parasitology, Dokkyo Medical University School of Medicine affil-num=3 en-affil= kn-affil=Center for Tropical Medicine and Parasitology, Dokkyo Medical University School of Medicine affil-num=4 en-affil= kn-affil=National Center for Parasitology, Entomology and Malaria Control, Ministry of Health affil-num=5 en-affil= kn-affil=National Center for Parasitology, Entomology and Malaria Control, Ministry of Health affil-num=6 en-affil= kn-affil=Institute of International Education and Research, Dokkyo Medical University School of Medicine affil-num=7 en-affil= kn-affil=Department of Human Ecology, Graduate School of Environmental Science, Okayama University, en-keyword=Schistosoma mekongi kn-keyword=Schistosoma mekongi en-keyword=Cambodia kn-keyword=Cambodia en-keyword=mathematical model kn-keyword=mathematical model en-keyword=Neotricula aperta kn-keyword=Neotricula aperta en-keyword=Mekong River kn-keyword=Mekong River END start-ver=1.4 cd-journal=joma no-vol=36 cd-vols= no-issue=3 article-no= start-page=117 end-page=123 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20043 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=High growing ability of Vibrio vulnificus biotype 1 is essential for production of a toxic metalloprotease causing systemic diseases in humans en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Vibrio vulnificus biotype 1, a causative agent of fatal septicemia or wound infection in humans, is known to produce a toxic metalloprotease as an important virulence determinant. V. vulnificus biotype 2 (serovar E), a primary eel pathogen, was found to elaborate an extracellular metalloprotease that was indistinguishable from that of biotype 1. The potential of V. vulnificus biotype 1 for production of the metalloprotease was compared with biotype 2 and other human non-pathogenic Vibrio species (Vibrio anguillarum and Vibrio proteolyticus). When cultivated at 25degreesC in tryptone-yeast extract broth supplemented with 0.9% NaCl, all bacteria multiplied sufficiently and secreted significant amounts of the metalloprotease. However, at 37degreesC with 0.9% NaCl, V. anguillarum neither grew nor produced the metalloprotease. In human serum, only V. vulnificus biotype 1 revealed a steady multiplication accompanied with production of the extracellular metalloprotease. This prominent ability of biotype 1 in growth and protease production may contribute to cause serious systemic diseases in humans.

en-copyright= kn-copyright= en-aut-name=WatanabeHirofumi en-aut-sei=Watanabe en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyoshiShin-ichi en-aut-sei=Miyoshi en-aut-mei=Shin-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawaseTomoka en-aut-sei=Kawase en-aut-mei=Tomoka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TomochikaKen-ichi en-aut-sei=Tomochika en-aut-mei=Ken-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShinodaSumio en-aut-sei=Shinoda en-aut-mei=Sumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama Univeristy affil-num=5 en-affil= kn-affil=Okayama University en-keyword=vibrio vulnificus kn-keyword=vibrio vulnificus en-keyword=metalloprotease kn-keyword=metalloprotease en-keyword=protease kn-keyword=protease END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=6 article-no= start-page=444 end-page=451 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20030729 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A model for the transmission of Echinococcus multilocularis in Hokkaido, Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A mathematical model for Echinococcus multilocularis transmission would be useful to estimate its prevalence and manage control strategies. We propose a mathematical model which quantitatively describes the transmission of E. multilocularis in Hokkaido, Japan. The model takes into account the influence of the dynamics of both the definitive and the intermediate host populations, which show large scale seasonal variations as they are wild animals. The simulations based on the model clarify the mechanism for the seasonal transmission of E. multilocularis quantitatively, notwithstanding a lack of seasonal prevalence data. At present, human alveolar echinococcosis is prevalent throughout the mainland of Hokkaido. The risk of being infected with alveolar echinococcosis in the human population has been investigated by analyzing the seasonal fluctuation of parasite egg dispersal in the environment, which should be necessary for planning more suitable preventive measures against E. multilocularis.

en-copyright= kn-copyright= en-aut-name=IshikawaHirofumi en-aut-sei=Ishikawa en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhgaYukio en-aut-sei=Ohga en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=DoiRikuo en-aut-sei=Doi en-aut-mei=Rikuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Horiba Limited affil-num=3 en-affil= kn-affil=Yokohama City University en-keyword=computer simulation kn-keyword=computer simulation en-keyword=echinococcus multilocularis kn-keyword=echinococcus multilocularis en-keyword=hokkaido kn-keyword=hokkaido en-keyword=japan kn-keyword=japan en-keyword=transmission model kn-keyword=transmission model END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mathematical modeling of Echinococcus multilocularis transmission en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A mathematical model for the transmission cycle of Echinococcus multilocularis would be useful for estimating its prevalence, and the model simulation can be instrumental in designing various control strategies. This review focuses on the epidemiological factors in the E. multilocularis transmission cycle and the recent advances of mathematical models for E. multilocularis transmission.

en-copyright= kn-copyright= en-aut-name=IshikawaHirofumi en-aut-sei=Ishikawa en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=Echinococcus multilocularis kn-keyword=Echinococcus multilocularis en-keyword=fox kn-keyword=fox en-keyword=mathematical model kn-keyword=mathematical model en-keyword=vole kn-keyword=vole END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200685 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The integrase interactor 1 (INI1) proteins facilitate Tat-mediated human immunodeficiency virus type 1 transcription en-subtitle= kn-subtitle= en-abstract= kn-abstract=Integration of human immunodeficiency virus type 1 (HIV-1) into the host genome is catalyzed by the viral integrase (IN) and preferentially occurs within transcriptionally active genes. During the early phase of HIV-1 infection, the incoming viral preintegration complex (PIC) recruits the integrase interactor 1 (INI1)/hSNF5, a chromatin remodeling factor which directly binds to HIV-1 IN. The impact of this event on viral replication is so far unknown, although it has been hypothesized that it could tether the preintegration complex to transcriptionally active genes, thus contributing to the bias of HIV integration for these regions of the genome. Here, we demonstrate that while INI1 is dispensable for HIV-1 transduction, it can facilitate HIV-1 transcription by enhancing Tat function. INI1 bound to Tat and both the repeat (Rpt) 1 and Rpt 2 domains of INI1 were required for efficient activation of Tat-mediated transcription. These results suggest that the incoming PICs might recruit INI1 to facilitate proviral transcription. en-copyright= kn-copyright= en-aut-name=AriumiYasuo en-aut-sei=Ariumi en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SerhanFatima en-aut-sei=Serhan en-aut-mei=Fatima kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TurelliPriscilla en-aut-sei=Turelli en-aut-mei=Priscilla kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TelentiAmalio en-aut-sei=Telenti en-aut-mei=Amalio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TronoDidier en-aut-sei=Trono en-aut-mei=Didier kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Ecole Polytechnique Fédérale de Lausanne affil-num=3 en-affil= kn-affil=Ecole Polytechnique Fédérale de Lausanne affil-num=4 en-affil= kn-affil=University of Lausanne affil-num=5 en-affil= kn-affil=Ecole Polytechnique Fédérale de Lausanne END start-ver=1.4 cd-journal=joma no-vol=188 cd-vols= no-issue=24 article-no= start-page=8376 end-page=8384 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modification of flagellin. Although the function of Orf3 remained unclear, an orf3 deletion mutant (Delta orf3 mutant) had reduced virulence on tobacco plants. Orf3 shows significant homology to a 3-oxoacyl-(acyl carrier protein) synthase III in the fatty acid elongation cycle. The Delta orf3 mutant had a significantly reduced ability to form acyl homoserine lactones (AHLs), which are quorum-sensing molecules, suggesting that Orf3 is required for AHL synthesis. In comparison with the wild-type strain, swarming motility, biosurfactant production, and tolerance to H2O2 and antibiotics were enhanced in the Delta orf3 mutant. A scanning electron micrograph of inoculated bacteria on the tobacco leaf surface revealed that there is little extracellular polymeric substance matrix surrounding the cells in the Delta orf3 mutant. The phenotypes of the Delta orf3 mutant and an AHL synthesis (Delta psyI) mutant were similar, although the mutant-specific characteristics were more extreme in the Delta orf3 mutant. The swarming motility of the Delta orf3 mutant was greater than that of the Delta psyI mutant. This was attributed to the synergistic effects of the overproduction of biosurfactants and/or alternative fatty acid metabolism in the Delta orf3 mutant. Furthermore, the amounts of iron and biosurfactant seem to be involved in biofilm development under quorum-sensing regulation in P. syringae pv. tabaci 6605. en-copyright= kn-copyright= en-aut-name=TaguchiFumiko en-aut-sei=Taguchi en-aut-mei=Fumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgawaYujiro en-aut-sei=Ogawa en-aut-mei=Yujiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakeuchiKasumi en-aut-sei=Takeuchi en-aut-mei=Kasumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SuzukiTomoko en-aut-sei=Suzuki en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University en-keyword=TO-CELL SIGNALS kn-keyword=TO-CELL SIGNALS en-keyword=AERUGINOSA kn-keyword=AERUGINOSA en-keyword=FLAGELLIN kn-keyword=FLAGELLIN en-keyword=BIOFILMS kn-keyword=BIOFILMS en-keyword=MOTILITY kn-keyword=MOTILITY en-keyword=IRON kn-keyword=IRON en-keyword=IDENTIFICATION kn-keyword=IDENTIFICATION en-keyword=SIDEROPHORES kn-keyword=SIDEROPHORES en-keyword=SPECIFICITY kn-keyword=SPECIFICITY en-keyword=FLUORESCENT kn-keyword=FLUORESCENT END start-ver=1.4 cd-journal=joma no-vol=189 cd-vols= no-issue=19 article-no= start-page=6945 end-page=6956 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200710 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Flagellin Glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose en-subtitle= kn-subtitle= en-abstract= kn-abstract=Flagellins from Pseudomonas syringae pv. glycinea race 4 and Pseudomonas syringae pv. tabaci 6605 have been found to be glycosylated. Glycosylation of flagellin is essential for bacterial virulence and is also involved in the determination of host specificity. Flagellin glycans from both pathovars were characterized, and common sites of glycosylation were identified on six serine residues (positions 143, 164, 176, 183, 193, and 201). The structure of the glycan at serine 201 (S201) of flagellin from each pathovar was determined by sugar composition analysis, mass spectrometry, and H-1 and C-13 nuclear magnetic resonance spectroscopy. These analyses showed that the S201 glycans from both pathovars were composed of a common unique trisaccharide consisting of two rhamnosyl (Rha) residues and one modified 4-amino-4,6-dideoxyglucosyl (Qui4N) residue, beta-D-Quip4N(3-hydroxy-1-oxobutyl)2Me-(1 -> 3)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap. Furthermore, mass analysis suggests that the glycans on each of the six serine residues are composed of similar trisaccharide units. Determination of the enantiomeric ratio of Rha from the flagellin proteins showed that flagellin from P. syringae pv. tabaci 6605 consisted solely Of L-Rha, whereas P. syringae pv. glycinea race 4 flagellin contained both L-Rha and D-Rha at a molar ratio of about 4:1. Taking these findings together with those from our previous study, we conclude that these flagellin glycan structures may be important for the virulence and host specificity of P. syringae. en-copyright= kn-copyright= en-aut-name=TakeuchiKasumi en-aut-sei=Takeuchi en-aut-mei=Kasumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OnoHiroshi en-aut-sei=Ono en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YoshidaMitsuru en-aut-sei=Yoshida en-aut-mei=Mitsuru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IshiiTadashi en-aut-sei=Ishii en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KatohEtsuko en-aut-sei=Katoh en-aut-mei=Etsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TaguchiFumiko en-aut-sei=Taguchi en-aut-mei=Fumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MikiRyuji en-aut-sei=Miki en-aut-mei=Ryuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MurataKatsuyoshi en-aut-sei=Murata en-aut-mei=Katsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KakuHanae en-aut-sei=Kaku en-aut-mei=Hanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=2 en-affil= kn-affil=National Food Research Institute affil-num=3 en-affil= kn-affil=National Food Research Institute affil-num=4 en-affil= kn-affil=Forestry and Forest Products Research Institute affil-num=5 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=6 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=8 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=9 en-affil= kn-affil=Faculty of Agriculture, Meiji University affil-num=10 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University en-keyword=INNATE IMMUNE-RESPONSE kn-keyword=INNATE IMMUNE-RESPONSE en-keyword=TOLL-LIKE RECEPTOR-5 kn-keyword=TOLL-LIKE RECEPTOR-5 en-keyword=PV. TABACI kn-keyword=PV. TABACI en-keyword=POSTTRANSLATIONAL MODIFICATION kn-keyword=POSTTRANSLATIONAL MODIFICATION en-keyword=BACTERIAL FLAGELLIN kn-keyword=BACTERIAL FLAGELLIN en-keyword=STRUCTURAL-ANALYSIS kn-keyword=STRUCTURAL-ANALYSIS en-keyword=AMINO-ACIDS kn-keyword=AMINO-ACIDS en-keyword=GLYCOSYLATION kn-keyword=GLYCOSYLATION en-keyword=AERUGINOSA kn-keyword=AERUGINOSA en-keyword=IDENTIFICATION kn-keyword=IDENTIFICATION END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue=3 article-no= start-page=729 end-page=732 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19956 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Automatic sensing device of electrical characteristics of living trees en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The electrical impedance of a living tissue reflects its cell construction and physiological activity. For this purpose we developed an automatic sensing device of electrical tissue characteristics. The system is composed of a part measuring impedance at multifrequency points and a part analyzing parameters of dispersion of bioelectrical impedance, impedances are measured at eight frequency points of 1 kHz-500 kHz. The parameters for Cole-Cole arc's law are determined automatically by a personal computer program

en-copyright= kn-copyright= en-aut-name=YamamotoYoshitake en-aut-sei=Yamamoto en-aut-mei=Yoshitake kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HaradaHiroshi en-aut-sei=Harada en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YasuharaKiyotaka en-aut-sei=Yasuhara en-aut-mei=Kiyotaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakamuraTakao en-aut-sei=Nakamura en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=biological techniques kn-keyword=biological techniques en-keyword=cellular biophysics kn-keyword=cellular biophysics en-keyword=computerised instrumentation kn-keyword=computerised instrumentation en-keyword=electric impedance kn-keyword=electric impedance en-keyword=measurement kn-keyword=measurement en-keyword=electric sensing devices kn-keyword=electric sensing devices END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Control and performance of a pulse-density-modulated series-resonant inverter for corona discharge processes en-subtitle= kn-subtitle= en-abstract= kn-abstract=

<p>This paper presents the control and performance of a pulse-density-modulated (PDM) series-resonant voltage-source inverter developed for corona discharge processes. The PDM inverter produces either a square-wave AC-voltage state or a zero-voltage state at its AC terminals to control the average output voltage under constant DC voltage and operating frequency. This results in a wide range of power control from 0.5% to 100%, even in the corona discharge load with a strong nonlinear characteristic. A 30 kHz 6 kW surface treatment system consisting of a voltage-source PDM inverter, a step-up transformer, and a corona discharge treater shows the establishment of a stable corona discharge in an extremely wide range of power control and, therefore, succeeds in performing both strong and weak surface treatment processes for film </p>

en-copyright= kn-copyright= en-aut-name=FujitaHideaki en-aut-sei=Fujita en-aut-mei=Hideaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=DC-AC power convertors kn-keyword=DC-AC power convertors en-keyword=corona kn-keyword=corona en-keyword=invertors kn-keyword=invertors en-keyword= load (electric) kn-keyword= load (electric) en-keyword=power transformers kn-keyword=power transformers en-keyword=resonant kn-keyword=resonant en-keyword=power convertors kn-keyword=power convertors en-keyword=surface treatment kn-keyword=surface treatment en-keyword=voltage control kn-keyword=voltage control END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue=1 article-no= start-page=354 end-page=363 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19971 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Control strategy and site selection of a shunt active filter for damping of harmonic propagation in power distribution systems en-subtitle= kn-subtitle= en-abstract= kn-abstract=

This paper deals with a shunt active filter which will be installed by an electric utility, putting much emphasis on the control strategy and the best point of installation of the shunt active filter on a feeder in a power distribution system. The objective of the shunt active filter is to damp harmonic propagation, which results from harmonic resonance between many capacitors for power factor improvement and line inductors in the feeder, rather than to minimize voltage distortion throughout the feeder. Harmonic mitigation is a welcome “by-product” of the shunt active filter, which comes from damping of harmonic propagation. This paper concludes that the shunt active filter based on detection of voltage at the point of installation is superior in stability to others, and that the best site selection is not the beginning terminal but the end terminal of the primary line in the feeder. Computer simulation is performed to verify the validity and effectiveness of the shunt active filter by means of an analog circuit simulator, which is characterized by installing it on a feeder of a radial distribution system in a residential area

en-copyright= kn-copyright= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=active filters kn-keyword=active filters en-keyword=distribution networks kn-keyword=distribution networks en-keyword=harmonic distortion kn-keyword=harmonic distortion en-keyword=power factor correction kn-keyword=power factor correction en-keyword=power filters kn-keyword=power filters en-keyword=power system control kn-keyword=power system control en-keyword=power system harmonics kn-keyword=power system harmonics en-keyword=power system stability kn-keyword=power system stability END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=586 end-page=591 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20017 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Motion segmentation by subspace separation and model selection en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Reformulating the Costeira-Kanade algorithm as a pure mathematical theorem independent of the Tomasi-Kanade factorization, we present a robust segmentation algorithm by incorporating such techniques as dimension correction, model selection using the geometric AIC, and least-median fitting. Doing numerical simulations, we demonstrate that oar algorithm dramatically outperforms existing methods. It does not involve any parameters which need to be adjusted empirically

en-copyright= kn-copyright= en-aut-name=KanataniKenichi en-aut-sei=Kanatani en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=14 cd-vols= no-issue=6 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Control and analysis of a unified power flow controller en-subtitle= kn-subtitle= en-abstract= kn-abstract=

<p>This paper presents a control scheme and comprehensive analysis for a unified power flow controller (UPFC) on the basis of theory, computer simulation and experiment. This developed theoretical analysis reveals that a conventional power feedback control scheme makes the UPFC induce power fluctuation in transient states. The conventional control scheme cannot attenuate the power fluctuation, and so the time constant of damping is independent of active and reactive power feedback gains integrated in its control circuit. This paper proposes an advanced control scheme which has the function of successfully damping out the power fluctuation. A UPFC rated at 10 kVA is designed and constructed, which is a combination of a series device consisting of three single-phase pulsewidth modulation (PWM) converters and a shunt device consisting of a three-phase diode rectifier. Although the dynamics of the shunt device are not included, it is possible to confirm and demonstrate the performance of the series device. Experimental results agree well with both analytical and simulated results and show viability and effectiveness of the proposed control scheme </p>

en-copyright= kn-copyright= en-aut-name=FujitaHideaki en-aut-sei=Fujita en-aut-mei=Hideaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeYasuhiro en-aut-sei=Watanabe en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=AC-DC power convertors kn-keyword=AC-DC power convertors en-keyword=PWM power convertors kn-keyword=PWM power convertors en-keyword=control system analysis computing kn-keyword=control system analysis computing en-keyword=control system synthesis kn-keyword=control system synthesis en-keyword=damping kn-keyword=damping en-keyword=feedback kn-keyword=feedback en-keyword=load flow control kn-keyword=load flow control en-keyword=power control kn-keyword=power control en-keyword=power kn-keyword=power en-keyword=system control kn-keyword=system control en-keyword=rectifying circuits kn-keyword=rectifying circuits END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=279 end-page=286 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19964 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pulse-density-modulated power control of a 4 kW, 450 kHz voltage-source inverter for induction melting applications en-subtitle= kn-subtitle= en-abstract= kn-abstract=

This paper presents a 4 kW, 450 kHz voltage-source inverter with a series resonant circuit for induction melting applications, which is characterized by the power control based on pulse density modulation (PDM). The pulse-density-modulated inverter makes an induction melting system simple and compact, thus leading to higher efficiency. A modulation strategy is proposed to realize the induction melting system capable of operation at the frequency and power level of interest. Some interesting experimental results are shown to verify the validity of the concept

en-copyright= kn-copyright= en-aut-name=FujitaHideki en-aut-sei=Fujita en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=DC-AC power convertors kn-keyword=DC-AC power convertors en-keyword=induction heating kn-keyword=induction heating en-keyword=melting kn-keyword=melting en-keyword=power control kn-keyword=power control en-keyword=pulse modulation kn-keyword=pulse modulation en-keyword=resonant power convertors kn-keyword=resonant power convertors END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue=2 article-no= start-page=345 end-page=356 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=19983 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The state-of-the-art of power electronics in Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Since the late 1950s, power electronics has been developing by leaps and bounds without saturation to become the key technology essential to modern society and human life as well as to electrical engineering. This paper mainly focuses on the state-of-the-art of power electronics technology and its medium to high-power applications because the author cannot survey the whole spectrum of power electronics ranging from a 5 W switching regulator to a 2.8 GW high-voltage DC transmission system now under construction in Japan. This paper also presents prospects and directions of power electronics in the 21st Century, including the personal views and expectations of the author

en-copyright= kn-copyright= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=power electronics kn-keyword=power electronics en-keyword=technological forecasting kn-keyword=technological forecasting END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue= article-no= start-page=29 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Modeling and damping of high-frequency leakage currents in PWM inverter-fed AC motor drive systems en-subtitle= kn-subtitle= en-abstract= kn-abstract=

This paper presents an equivalent circuit for high-frequency leakage currents in pulsewidth modulation (PWM) inverter-fed AC motors, which forms a series resonant circuit. The analysis based on the equivalent circuit leads to such a conclusion that the connection of a conventional common-mode choke or reactor in series between the AC terminals of a PWM inverter and those of an AC motor is not effective to reduce the rms and average values of the leakage current, but effective to reduce the peak value. Furthermore, this paper proposes a common-mode transformer which is different in damping principle from the conventional common-mode choke. It is shown theoretically and experimentally that the common-mode transformer is able to reduce the rms value of the leakage current to 25%, where the core used in the common-mode transformer is smaller than that of the conventional common-mode choke

en-copyright= kn-copyright= en-aut-name=OgasawaraSatoshi en-aut-sei=Ogasawara en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=AC motor drives kn-keyword=AC motor drives en-keyword=PWM invertors kn-keyword=PWM invertors en-keyword=circuit resonance kn-keyword=circuit resonance en-keyword=damping kn-keyword=damping en-keyword=equivalent circuits kn-keyword=equivalent circuits en-keyword=inductors kn-keyword=inductors en-keyword=leakage currents kn-keyword=leakage currents en-keyword=resonance kn-keyword=resonance en-keyword=transformers kn-keyword=transformers END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=1 article-no= start-page=131 end-page=138 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20062 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A micro ultrasonic motor using a micro-machined cylindrical bulk PZT transducer en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In this paper, a micro ultrasonic motor using a micro-machined bulk piezoelectric transducer is introduced. The cylindrical shaped bulk piezoelectric transducer, a diameter of 0.8 mm and a height of 2.2 mm, was developed as stator transducer for traveling wave type ultrasonic motor. The transducer was made of lead zirconate titanate (PZT) bulk ceramics, and formed by micro machining, Ni plating and laser beam cutting process. Using this stator transducer, we have fabricated a cylindrical micro ultrasonic motor, a diameter of 2.0 mm and a height of 5.9 mm. We have also evaluated some characteristics and succeeded in driving the micro ultrasonic motor.

en-copyright= kn-copyright= en-aut-name=KandaTakefumi en-aut-sei=Kanda en-aut-mei=Takefumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MakinoAkira en-aut-sei=Makino en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OnoTomohisa en-aut-sei=Ono en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SuzumoriKoichi en-aut-sei=Suzumori en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoritaTakeshi en-aut-sei=Morita en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KurosawaMinoru Kuribayashi en-aut-sei=Kurosawa en-aut-mei=Minoru Kuribayashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=University of Tokyo affil-num=6 en-affil= kn-affil=Tokyo Institute of Technology en-keyword=Piezoelectric actuator kn-keyword=Piezoelectric actuator en-keyword=Ultrasonic motor kn-keyword=Ultrasonic motor en-keyword=Micro motor kn-keyword=Micro motor en-keyword=Bulk piezoelectric material kn-keyword=Bulk piezoelectric material en-keyword=Micro machining kn-keyword=Micro machining END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue= article-no= start-page=32 end-page=39 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Control and performance of a flywheel energy storage system based on a doubly-fed induction generator-motor for power conditioning en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A flywheel energy storage system based on a doubly-fed induction generator-motor basically consists of a wound-rotor induction machine and a cycloconverter or a voltage-source PWM rectifier-inverter which is used as an AC exciter. Adjusting the rotor speed makes the generator-motor either release the kinetic energy to the power system or absorb it from the utility grid. Thus, the generator-motor has the capability of achieving not only reactive power control, but also active power control based on the flywheel effect of the rotating parts. This paper proposes a new control strategy for a doubly-fed induction generator-motor, which is characterized by the combination of vector control and decoupling control. The control strategy enables the induction generator-motor to perform active power control independent of reactive power control even in transient states. The validity of the theory developed in this paper, along with the effectiveness and viability of the control strategy, is confirmed by computer simulation. In addition, this paper discusses a transient behavior of a magnetizing current in the induction machine

en-copyright= kn-copyright= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SatoHikaru en-aut-sei=Sato en-aut-mei=Hikaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=PWM invertors kn-keyword=PWM invertors en-keyword=asynchronous generators kn-keyword=asynchronous generators en-keyword=exciters kn-keyword=exciters en-keyword=flywheels kn-keyword=flywheels en-keyword=induction motors kn-keyword=induction motors en-keyword=machine vector control kn-keyword=machine vector control en-keyword=reactive power control kn-keyword=reactive power control en-keyword=rotors kn-keyword=rotors END start-ver=1.4 cd-journal=joma no-vol=14 cd-vols= no-issue=6 article-no= start-page=1021 end-page=1027 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Control and analysis of a unified power flow controller en-subtitle= kn-subtitle= en-abstract= kn-abstract=

This paper presents a control scheme and comprehensive analysis for a unified power flow controller (UPFC) on the basis of theory, computer simulation and experiment. This developed theoretical analysis reveals that a conventional power feedback control scheme makes the UPFC induce power fluctuation in transient states. The conventional control scheme cannot attenuate the power fluctuation, and so the time constant of damping is independent of active and reactive power feedback gains integrated in its control circuit. This paper proposes an advanced control scheme which has the function of successfully damping out the power fluctuation. A UPFC rated at 10 kVA is designed and constructed, which is a combination of a series device consisting of three single-phase pulsewidth modulation (PWM) converters and a shunt device consisting of a three-phase diode rectifier. Although the dynamics of the shunt device are not included, it is possible to confirm and demonstrate the performance of the series device. Experimental results agree well with both analytical and simulated results and show viability and effectiveness of the proposed control scheme

en-copyright= kn-copyright= en-aut-name=FujitaHideaki en-aut-sei=Fujita en-aut-mei=Hideaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeYasuhiro en-aut-sei=Watanabe en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page=430 end-page=436 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20019 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A global routing technique for wave-steered design methodology en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Wave-Steering is a new circuit design methodology to realize high throughput circuits by embedding layout friendly structures in silicon. Latches guarantee correct signal arrival times at the input of synthesized modules and maintain the high throughput of operation. This paper presents a global routing technique for networks of wave-steered blocks. Latches can be distributed along interconnects. Their number depends on net topologies and signal ordering at the inputs of wave steered blocks. here, we route nets using Steiner tree heuristics and determine signal ordering and latch positions on interconnect. The problem of total latch number minimization is solved using SAT formulation. Experimental results on benchmark circuits show the efficiency of our technique. We achieve on average a 40% latch reduction at minimum latency over un-optimized circuits operating at 250 MHz in 0.25 μm CMOS technology

en-copyright= kn-copyright= en-aut-name=FunabikiNobuo en-aut-sei=Funabiki en-aut-mei=Nobuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SinghAmit en-aut-sei=Singh en-aut-mei=Amit kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MukherjeeArindam en-aut-sei=Mukherjee en-aut-mei=Arindam kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SadowskaMalgorzata Marek en-aut-sei=Sadowska en-aut-mei=Malgorzata Marek kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=University of California affil-num=3 en-affil= kn-affil=University of California affil-num=4 en-affil= kn-affil=University of California en-keyword=binary decision diagrams kn-keyword=binary decision diagrams en-keyword=circuit layout CAD kn-keyword=circuit layout CAD en-keyword=flip-flops kn-keyword=flip-flops en-keyword=integrated circuit layout kn-keyword=integrated circuit layout en-keyword=network routing kn-keyword=network routing END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue= article-no= start-page=1170 end-page=1175 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198910 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A control strategy of three-phase PWM inverter with fluctuating input voltage en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A digital control strategy for the three-phase PWM inverter is proposed for a sinusoidal output with a fluctuating input voltage. The pulse width is computed by sampling and predicting the fluctuating input voltage every time the pulse generates. Three predictive methods are proposed. The errors and the waveform distortion of the output voltage are analyzed and discussed, along with the proposed predictive methods. The validity of the control method was experimentally verified using a microprocessor-based control system

en-copyright= kn-copyright= en-aut-name=FunabikiShigeyuki en-aut-sei=Funabiki en-aut-mei=Shigeyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=computerised control kn-keyword=computerised control en-keyword=invertors kn-keyword=invertors en-keyword=microcomputer applications kn-keyword=microcomputer applications en-keyword=pulse width modulation kn-keyword=pulse width modulation END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue=2 article-no= start-page=315 end-page=322 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=19983 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The unified power quality conditioner: the integration of series and shunt-active filters en-subtitle= kn-subtitle= en-abstract= kn-abstract=

This paper deals with unified power quality conditioners (UPQCs), which aim at the integration of series-active and shunt-active power filters. The main purpose of a UPQC is to compensate for voltage flicker/imbalance, reactive power, negative-sequence current and harmonics. In other words, the UPQC has the capability of improving power quality at the point of installation on power distribution systems or industrial power systems. This paper discusses the control strategy of the UPQC, with a focus on the how of instantaneous active and reactive powers inside the UPQC. Experimental results obtained from a laboratory model of 20 kVA, along with a theoretical analysis, are shown to verify the viability and effectiveness of the UPQC

en-copyright= kn-copyright= en-aut-name=FujitaHideaki en-aut-sei=Fujita en-aut-mei=Hideaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University en-keyword=active filters kn-keyword=active filters en-keyword=distribution networks kn-keyword=distribution networks en-keyword=harmonic distortion kn-keyword=harmonic distortion en-keyword=industrial power systems kn-keyword=industrial power systems en-keyword=power filters kn-keyword=power filters en-keyword=power supply quality kn-keyword=power supply quality en-keyword=power system harmonics kn-keyword=power system harmonics END