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ID 16379
Eprint ID
16379
FullText URL
95_929.pdf 458 KB
Title Alternative
Studies on the pathogenetic mechanism in autoimmune thyroid disorders Part 1 Studies on the cytotoxic factor by (86)Rb exchange method
Author
Miura, Hiroshi
Abstract
The cytotoxic factor to cultured thyroid cells in sera from patients with autoimmune thyroid diseases was studied by the (86)Rb exchange method which was simple and quantitative. Kinetic analysis of cell destruction indicated that cytolysis was a rapid event and that most lysis occurred during the first 12 hours. The susceptibility of thyroid cells from Graves' thyroids to the cytotoxic factor was variable. Twenty out of 21 serum samples from patients with Hashimoto's disease and 10 out of 12 serum samples from patients with Graves' disease showed cytotoxic effects on thyroid cells in culture. The percentage of killing of sera from patients with Hashimoto's thyroiditis and with Graves' disease was significantly higher than that of normal individuals (p<0.001). The percentage of killing correlated with the anti-microsomal antibody (MCHA) titer (r=0.68, p<0.001), but not with the anti-thyroglobulin antibody (TGHA) titer. Serum absorption studies with various thyroid subcellular fractions revealed that the cytotoxic activity of sera was reduced markedly after absorption with thyroid microsome or plasma membrane fractions, but not with the thyroglobulin fraction, suggesting that the antigen to the cytotoxic factor was expressed in the plasma membrane.
Keywords
甲状腺上皮細胞
cytotoxic factor
(86)Rb
Published Date
1983-10-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume95
Issue
issue9-10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
929
End Page
937
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/95/9-10/95_9-10_929/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
Japanese
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma