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ID 31346
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Author
Oda, Takuzo
Seki, Shuji
Okazaki, Hiroaki
Abstract

New colorimetric methods for the estimation of the activities of cytochrome c oxidase and of cytochrome c-cytochrome oxidase system in tissue homogenates are described, using neotetrazolium chloride in the presence of p-phenylenediamine with or without additional cytochrome c. Optimal time of incubation, optimal concentration of the incubation medium and amounts of tissue, and simple method for the extraction of the reduced neotetrazolium chloride were determined. The reduction of neotetrazolium chloride was proportional to the amount of enzyme. Using this method, colorimetric estimations of cytochrome c-cytochrome oxidase system activity in the kidney, heart, liver, brains, and skeletal muscle were made. The procedures of these methods are very simple, and they are considered to be feasible in the combination with histochemical demonstrations of these enzyme activities.

Amo Type
Article
Publication Title
Acta Medicinae Okayama
Published Date
1958-12
Volume
volume12
Issue
issue4
Publisher
Okayama University Medical School
Start Page
293
End Page
301
NCID
AA00041342
Content Type
Journal Article
language
English
File Version
publisher
Refereed
True
NAID