Transaminase検量線について

Of late clinical assay of transaminase has gained a greater importance and the methods of the assay are now being studied from various angles. As for making the standard curves there are such methods as that of Reitman and Mizobe's, the latter being very similar to the former, but there still remain problems that need to be solved in these methods with respect to the quantity of 2.4-dinitrophenylhydrazine solution to be added. With the purpose to clarify these points, the author conducted a series of experiments and demonstrated theoretically and clinically that the addition of 2.4-dinitrophenylhydrazine solution in the quantity more than twice the amounts employed by various investigators would yield a perfectly straight line curve in contrast to the available reports where the addition of pyruvate in the concentrations up to 0.3 μM as reported by Mizobe and others gives a straight line curve but any quantity above that tends to distort the straight line.