| ID | 4688 |
| Eprint ID | 4688
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| FullText URL | |
| Title Alternative | ラット海馬の錐体細胞におけるDNAトポイソメラーゼⅡβ発現の領域による違い
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| Author | |
| Abstract | A detailed analysis of the differential expression of a nuclear enzyme, DNA topoisomerase (topo) IIβ, was performed in the rat hippocampal pyramidal layer. Three-dimensional (3-D) reconstruction from serial sections immunostained with anti-topo IIβ antibody showed that the immunoreactivity was apparently weak in the entire CA3 region. Almost all CA1 pyramidal cells showed similar immunoreactivity to that seen in the dentate granular cells, the subicular neurons, and the cerebral neocortical neurons. In addition, immunoblotting analysis in the adult dorsal hippocampus showed that the expression level of topo IIβ in the CA3 was significantly lower than that in the CA1 region. The dissociation in the expression level between CA1 and CA3 occurred in postnatal days 4 (P4) through P6. The present finding suggests that the enzyme is possibly involved in activities of the hippocampal pyramidal neurons.
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| Keywords | DNA topoisomerase IIβ
Hippocampal pyramidal cells
Immunohistochemistry
3-D reconstruction
Western blotting
Postnatal development
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| Note | 掲載順位 77
http://dx.doi.org/10.1016/S0168-0102(00)00098-5
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| Published Date | 2000-03-31
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| Publication Title | |
| Content Type |
Thesis or Dissertation
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| Grant Number | 甲第2093号
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| Granted Date | 2000-03-31
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| Thesis Type |
Doctor of Philosophy in Medical Science
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| Grantor | 岡山大学
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| Official Url | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10771107&dopt=Abstract
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| language |
Japanese
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| File Version | none
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| Refereed |
Unknown
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