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ID 17330
Eprint ID
17330
FullText URL
87_131.pdf 1.76 MB
Title Alternative
COMPLETE DIGESTION OF 5S RIBOSOMAL RNA FROM A MOUSE HEPATOMA CELL WITH SPECIFIC RIBONUCLEASES
Author
Takai, Kenichi
Abstract
Ribosomal 5S RNA was purified from a hepatoma (MH134) of C3H/He mice labeled with (32)P. The purified 5S RNA was digested with pancreatic RNase and RNase T(1), and their endproducts were separated with two dimensional electrophoresis to determine their nucleotide sequences. The molecule had uridine at its 3'-terminus and guanylic acid its 5'-terminus. Except for the presence of a 5'-terminal diphosphate, these nucleotide sequences were identical with those of KB cells, confirming the identity of the primary structure of 5S ribosomal RNA between them. The significance of 5'-terminal diphosphate in relation to the biosynthesis of 5S ribosomal RNA was also discussed.
Published Date
1975-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume87
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
131
End Page
145
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/87/1-2/87_1-2_131/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
Japanese
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma