result 1650 件
| JaLCDOI | 10.18926/AMO/63407 |
|---|---|
| FullText URL | 76_2_137.pdf |
| Author | Tsukahara, Masaru| So, Ryuhei| Yada, Yuji| Kodama, Masafumi| Kishi, Yoshiki| Yamada, Norihito| |
| Abstract | Although reported for Caucasians, changes in plasma clozapine levels after smoking cessation in East Asians remain unclear. We here investigated plasma clozapine levels before and after smoking cessation in Japanese inpatients with schizophrenia. We conducted a retrospective chart review of 14 inpatients with schizophrenia who were being treated with clozapine between June 1, 2019, and July 31, 2019 and who were smokers as of July 1, 2019, the day on which a smoking ban was instituted in the tertiary public psychiatric hospital. The primary outcome was individual differences in plasma clozapine levels between before and after the smoking ban, which were compared using paired t-tests. The mean plasma clozapine level was significantly increased, by 213.4 ng/mL (95% CI 119.9-306.8; p<0.01) or 53.2%. Four of the 14 inpatients experienced clinically significant side effects, such as myoclonus, drooling, and amnesia, due to the development of high plasma clozapine levels. Our findings indicated that close monitoring of plasma clozapine levels before and after smoking cessation and prior dose adjustment of clozapine may be necessary, to prevent a significant risk of developing high plasma clozapine levels, even in Japanese patients. |
| Keywords | Asian clozapine schizophrenia smoking |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-04 |
| Volume | volume76 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 137 |
| End Page | 143 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35503441 |
| Web of Science KeyUT | 000792374900005 |
| JaLCDOI | 10.18926/AMO/63405 |
|---|---|
| FullText URL | 76_2_121.pdf |
| Author | Okazaki, Yuki| Furumatsu, Takayuki| Hiranaka, Takaaki| Kamatsuki, Yusuke| Nakata, Eiji| Tetsunaga, Tomonori| Yamane, Kentaro| Ozaki, Toshifumi| |
| Abstract | Bone marrow edema (BME) after meniscus injury and risk factors for subchondral insufficiency fracture of the knee (SIFK) have been reported. However, their association with medial meniscus posterior root tear (MMPRT) remains unknown. We investigated the association of BME volume (BME-V), posterior shinycorner lesion (PSCL), and SIFK with MMPRT to examine the correlations between BME-V and medial meniscus extrusion (MME), PSCL and duration from injury to the time of magnetic resonance imaging (duration), and SIFK and duration. Twenty-nine patients who underwent surgery for MMPRT were included (mean age, 59.2; range, 39-84). The presence of PSCL, femoral BME-V (cm3), and SIFK grade (1-4) were evaluated. Preoperative factors, such as MME (mm) and duration (weeks), were investigated using multivariate linear/ logistic regression analyses. Multivariate linear regression analysis revealed duration as a significant factor for high-grade SIFK (p<0.01). Multivariate logistic regression analysis revealed duration as a significant factor for the presence of PSCL (odds ratio=0.94, p<0.05). A long duration of MMPRT leads to severe MME and highgrade SIFK (3 and 4), often resulting in knee arthroplasty. Early diagnosis of MMPRT and pullout repair can prevent severe MME and high-grade SIFK. |
| Keywords | medial meniscus posterior root tear subchondral insufficiency fracture bone marrow edema meniscus extrusion |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-04 |
| Volume | volume76 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 121 |
| End Page | 127 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35503439 |
| Web of Science KeyUT | 000792374900003 |
| JaLCDOI | 10.18926/AMO/63403 |
|---|---|
| FullText URL | 76_2_105.pdf |
| Author | Takaoka, Megumi| Ohsumi, Shozo| Ikejiri, Haruka| Shidahara, Tomohiro| Miyoshi, Yuichiro| Takahashi, Mina| Takashima, Seiki| Aogi, Kenjiro| |
| Abstract | Cases of breast cancer metastasis after achieving a pathological complete response (pCR) with neoadjuvant chemotherapy (NAC) are sometimes encountered in clinical practice. We investigated the prognostic factors for pCR in patients with breast cancer after NAC. This retrospective cohort study included patients with localized breast cancer who underwent NAC followed by surgery between 2004 and 2020 and achieved a pCR. The associations between clinical factors and distant metastasis-free survival rate were statistically analyzed. We analyzed data for 127 patients. Twelve patients (9.4%) had distant metastases, and seven (5.5%) died. For estrogen receptor (ER)-positive patients, the distant metastasis-free survival rate was 94.6% for both 5 and 8 years. In contrast, ER-negative patients had a distant metastasis-free survival rate of 87.6% and 85.4% for 5 and 8 years (p=0.094), respectively. In cT0-2 patients, the distant metastasis-free survival rate was 92.4% for 5 years and 90.5% for 8 years, whereas in cT3-4 patients, the distant metastasis-free survival rate was 83.5% for 5 years and 83.5% for 8 years (p=0.301). This study suggested that patients with ER-negative, pre-NAC cT3 or T4 breast cancer who had achieved a pCR after NAC tended to have a worse prognosis. |
| Keywords | breast carcinoma neoadjuvant therapy prognosis |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-04 |
| Volume | volume76 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 105 |
| End Page | 111 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35503437 |
| Web of Science KeyUT | 000792374900001 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Inagaki, Junko| Nakano, Airi| Hatipoglu, Omer Faruk| Ooka, Yuka| Tani, Yurina| Miki, Akane| Ikemura, Kentaro| Opoku, Gabriel| Ando, Ryosuke| Kodama, Shintaro| Ohtsuki, Takashi| Yamaji, Hirosuke| Yamamoto, Shusei| Katsuyama, Eri| Watanabe, Shogo| Hirohata, Satoshi| |
| Keywords | osteoarthritis matrix metalloproteinase MMP13 ADAMTS9 expression screening chondrocytes |
| Published Date | 2022-02-28 |
| Publication Title | International Journal Of Molecular Sciences |
| Volume | volume23 |
| Issue | issue5 |
| Publisher | MDPI |
| Start Page | 2681 |
| ISSN | 1422-0067 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 by the authors. |
| File Version | publisher |
| PubMed ID | 35269821 |
| DOI | 10.3390/ijms23052681 |
| Web of Science KeyUT | 000773522200001 |
| Related Url | isVersionOf https://doi.org/10.3390/ijms23052681 |
| Title Alternative | Downregulation of PAR-1 and PAR-2 by Rivaroxaban |
|---|---|
| FullText URL | fulltext20220309-4.pdf Figure20220309-4.pdf |
| Author | Morihara, Ryuta| Yamashita, Toru| Kono, Syoichiro| Shang, Jingwei| Nakano, Yumiko| Sato, Kota| Hishikawa, Nozomi| Ohta, Yasuyuki| Heitmeier, Stefan| Perzborn, Elisabeth| Abe, Koji| |
| Keywords | PAR-3 PAR-4 tissue plasminogen activator warfarin |
| Note | This is the peer reviewed version of the following article: [Morihara, R., Yamashita, T., Kono, S., Shang, J., Nakano, Y., Sato, K., Hishikawa, N., Ohta, Y., Heitmeier, S., Perzborn, E. and Abe, K. (2017), Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2. Journal of Neuroscience Research, 95: 1818-1828. https://doi.org/10.1002/jnr.24013], which has been published in final form at [https://doi.org/10.1002/jnr.24013]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages there of by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.| |
| Published Date | 2016-12-30 |
| Publication Title | Journal of Neuroscience Research |
| Volume | volume95 |
| Issue | issue9 |
| Publisher | Wiley |
| Start Page | 1818 |
| End Page | 1828 |
| ISSN | 0360-4012 |
| NCID | AA00703378 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | ©2016 Wiley Periodicals, Inc. |
| File Version | author |
| DOI | 10.1002/jnr.24013 |
| Web of Science KeyUT | 000427450300275 |
| Related Url | isVersionOf https://doi.org/10.1002/jnr.24013 |
| JaLCDOI | 10.18926/AMO/63219 |
|---|---|
| FullText URL | 76_1_99.pdf |
| Author | Naito, Yoichiro| Yoshikawa, Masaki| Nakamura, Kazufumi| Kubo, Motoki| Sugiyama, Hiroyasu| Suzuki, Hideyuki| Fujita, Shinpei| Arai, Yasunori| Takahashi, Sho| Kato, Yuichi| Yoshida, Yu| Akai, Hiroaki| Murakami, Shuhei| Ito, Hiroshi| |
| Abstract | Rapidly progressive in-stent restenosis (ISR) after stent deployment from the left main trunk (LMT) to the left anterior descending artery (LAD) without plaque at the LMT ostium has not been reported. A 60-year-old Japanese man with a history of scleroderma, pulmonary fibrosis, and type 2 diabetes developed acute myocardial infarction of the right coronary artery (RCA) and was treated by emergency percutaneous coronary intervention (PCI) for RCA. Nine days later he underwent PCI from the LMT to the LAD. Follow-up coronary angiography (CAG) at 9 and 21 months post-PCI did not reveal ISR in any lesion, but the patient experienced cardiac arrest at 25 months post-PCI. Emergency CAG after resuscitation revealed ISR of the LMT ostium; emergency PCI was conducted. The development of ISR at the ostium of the LMT although the patient was free of plaque 4 months before is extremely unusual. This rare ISR of the LMT ostium progressed rapidly after follow-up CAG revealed no ISR at 21 months post-stent implantation. |
| Keywords | left main trunk in-stent restenosis cardiopulmonary arrest |
| Amo Type | Case Report |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 99 |
| End Page | 104 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35237006 |
| Web of Science KeyUT | 000762933000003 |
| JaLCDOI | 10.18926/AMO/63214 |
|---|---|
| FullText URL | 76_1_71.pdf |
| Author | Tanaka, Masato| Sonawane, Sumeet| Fujiwara, Yoshihiro| Uotani, Koji| Arataki, Shinya| Yamauchi, Taro| Ye, Youchen| Misawa, Haruo| |
| Abstract | The surgical treatment of pediatric atlantoaxial subluxation (AAS) in Down syndrome (DS) remains technically challenging due to radiation exposure and complications such as vertebral artery injury and nonunion. The established treatment is fixation with a C1 lateral mass screw and C2 pedicle screw (modified Goel technique). However, this technique requires fluoroscopy for C1 screw insertion. To avoid exposing the operating team to radiation we present here a new C-arm free O-arm navigated surgical procedure for pediatric AAS in DS. A 5-year-old male DS patient had neck pain and unsteady gait. Radiograms showed AAS with an atlantodental interval of 10 mm, and irreducible subluxation on extension. CT scan showed Os odontoideum and AAS. MRI demonstrated spinal cord compression between the C1 posterior arch and odontoid process. We performed a C-arm free O-arm navigated modified Goel procedure with postoperative halo-vest immobilization. At oneyear follow-up, good neurological recovery and solid bone fusion were observed. The patient had no complications such as epidural hematoma, infection, or nerve or vessel injury. This novel procedure is a useful and safe technique that protects surgeons and staff from radiation risk. |
| Keywords | atlantoaxial fixation Down syndrome C-arm free O-arm navigation surgery modified Goel technique |
| Amo Type | Case Report |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 71 |
| End Page | 78 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35237001 |
| Web of Science KeyUT | 000762803800001 |
| JaLCDOI | 10.18926/AMO/63207 |
|---|---|
| FullText URL | 76_1_41.pdf |
| Author | Miyahara, Hiroyuki| Miyai, Takayuki| Aya, Kunihiko| Tsukahara, Hirokazu| |
| Abstract | High-dose steroids are required for the treatment of minimal change nephrotic syndrome (MCNS), especially for episodes of recurrence. Predicting and avoiding recurrence can help reduce the steroid dose, but prediction is currently difficult. We herein examined whether changes in laboratory data, especially the urinary protein- to-creatinine ratio (UTP/UCr), can predict clinical recurrence. We also assessed differences in clinical features between children and young adults. We included 36 patients with MCNS; for each case, we retrospectively studied laboratory data during stable remission and pre-recurrence, with the “stable” period defined as all but the 6 weeks before recurrence, and pre-recurrence defined as the 4±2 weeks before recurrence. UTP/UCr, serum albumin, etc. were measured every 5 years during stable periods. We divided patients into cohorts by age at recurrence, < 15 years and ≥ 15 years, and compared stable and pre-recurrence values for the two groups. UTP/UCr values during stable periods tended to be higher in younger patients. UTP/UCr and serum albumin showed statistically significant changes during pre-recurrence periods, but only in those aged ≥ 15 years. Thus, clinical features of recurrence differed depending on age. Signs of recurrence can be confirmed via UTP/UCr or serum albumin several weeks before recurrence in patients ≥ 15 years. |
| Keywords | minimal change nephrotic syndrome recurrence urinary protein to creatinine ratio |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 41 |
| End Page | 49 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35236997 |
| Web of Science KeyUT | 000762812700006 |
| JaLCDOI | 10.18926/AMO/63206 |
|---|---|
| FullText URL | 76_1_33.pdf |
| Author | Zhou, Jia-Yi| Hou, Hai-Tao| Wang, Shi-Fu| Yang, Qin| He, Guo-Wei| |
| Abstract | Trace elements selenium (Se) and cobalt (Co) are essential in the human body, and a correlation between Se and cardiac surgery has been suggested. We investigated the plasma concentrations of Se and Co during and after coronary artery bypass grafting (CABG) surgery under cardiopulmonary bypass (CPB). From December 2019 to January 2020, preoperative plasma samples from isolated first-time CABG patients (n=20; 10 males, 10 females) were prospectively collected post-anesthesia and before CPB (T1), 45 min after CPB started (T2), 90 min after CPB started (T3), and postoperative days 1 (T4), and day 4 (T5). The plasma concentrations of Se and Co were measured. The Se concentration was significantly decreased at T2 (105.24±4.08 vs. 68.56±2.42 μg/L, p<0.001) and T3 (105.24±4.08 vs. 80.41±3.40 μg/L, p<0.001). The Co concentration was significantly decreased at T4 (0.35±0.19 vs. 0.26±0.13 μg/L, p<0.01) and T5 (0.35±0.19 vs. 0.23±0.11 μg/L, p<0.001). Five patients developed atrial fibrillation (AF); there was no other operative mortality or major morbidity. This is the first report of alterations of plasma Se and Co concentrations during and after CABG surgery. Our results may indicate that Se supplementation before or during CABG and Co supplementation after CABG may become necessary for patients undergoing CABG. |
| Keywords | trace element CABG cardiopulmonary bypass selenium cobalt |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 33 |
| End Page | 39 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35236996 |
| Web of Science KeyUT | 000762812700005 |
| JaLCDOI | 10.18926/AMO/63205 |
|---|---|
| FullText URL | 76_1_25.pdf |
| Author | Sugimoto, Kohei| Kuroda, Masahiro| Yoshimura, Yuuki| Hamada, Kentaro| Khasawneh, Abdullah| Barham, Majd| Tekiki, Nouha| Konishi, Kohei| Ishizaka, Hinata| Shimizu, Yudai| Nakamitsu, Yuki| Al-Hammad, Wlla E. | Kamizaki, Ryo| Kanazawa, Susumu| Asaumi, Junichi| |
| Abstract | The apparent diffusion coefficient subtraction method (ASM) was developed as a new restricted diffusionweighted imaging technique for magnetic resonance imaging (MRI). The usefulness of the ASM has been established by in vitro basic research using a bio-phantom, and clinical research on the application of the ASM for the human body is needed. Herein, we developed a short-time sequence for ASM imaging of the heads of healthy volunteers (n=2), and we investigated the similarity between the obtained ASM images and diffusion kurtosis (DK) images to determine the utility of the ASM for clinical uses. This study appears to be the first to report ASM images of the human head. We observed that the short-time sequence for the ASM imaging of the head can be scanned in approx. 3 min at 1.5T MRI. The noise reduction effect of median filter processing was confirmed on the ASM images scanned by this sequence. The obtained ASM images showed a weak correlation with the DK images, indicating that the ASM images are restricted diffusion-weighted images. The new shorttime imaging sequence could thus be used in clinical studies applying the ASM. |
| Keywords | apparent diffusion coefficient apparent diffusion coefficient subtraction method diffusion kurtosis imaging restricted diffusion short-time imaging |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 25 |
| End Page | 32 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35236995 |
| Web of Science KeyUT | 000762812700004 |
| JaLCDOI | 10.18926/AMO/63204 |
|---|---|
| FullText URL | 76_1_17.pdf |
| Author | Fujishita, Keigo| Yasuhisa, Sando| Oka, Satoshi| Fujisawa, Yuka| Machida, Takuya| Imai, Toshi| |
| Abstract | R-CHOP therapy is generally performed every 3 weeks. We investigated the effects of extending the interval of R-CHOP therapy for > 1 week on the prognoses of patients with non-indolent non-Hodgkin’s B-cell lymphoma. Among the 338 patients with non-indolent non-Hodgkin’s B-cell lymphoma who received initial chemotherapy at our institution, we focused on 178 patients who received R-CHOP therapy and analyzed the outcomes of the patients stratified by the treatment intervals. The estimated 3-year overall survival (OS) for the entire population was 82.1%. Patients treated at intervals of ≥ 4 weeks were significantly older, and they had significantly longer follow-up periods and lower relative dose intensity. But the estimated 3-year OS was comparable to those treated at <4 weeks (83.3% vs. 80.5% p=0.947). In a multivariate analysis, age and the dose of anti-cancer agents had significant impacts on OS, but there was no significant relationship regarding the treatment intervals. Propensity score matching confirmed the same result. R-CHOP therapy every around 4 weeks could achieve relatively good survival in some selected patients with non-indolent non-Hodgkin’s B-cell lymphoma. |
| Keywords | R-CHOP therapy relative dose intensity non-Hodgkin’s lymphoma |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 17 |
| End Page | 24 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35236994 |
| Web of Science KeyUT | 000762812700003 |
| JaLCDOI | 10.18926/AMO/63202 |
|---|---|
| FullText URL | 76_1_1.pdf |
| Author | Takao, Tomoka| Yamada, Daisuke| Takarada, Takeshi| |
| Abstract | Optogenetics, a technology to manipulate biological phenomena thorough light, has attracted much attention in neuroscience. Recently, the Magnet System, a photo-inducible protein dimerization system which can control the intracellular behavior of various biomolecules with high accuracy using light was developed. Furthermore, photoactivation systems for controlling biological phenomena are being developed by combining this technique with genome-editing technology (CRISPR/Cas9 System) or DNA recombination technology (Cre-loxP system). Herein, we review the history of optogenetics and the latest Magnet System technology and introduce our recently developed photoactivatable Cre knock-in mice with temporal-, spatial-, and cell-specific accuracy. |
| Keywords | optogenetics Cre recombinase |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 1 |
| End Page | 5 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35236992 |
| Web of Science KeyUT | 000762812700001 |
| Title Alternative | Studies on antitumor enzyme l-lysine α-oxidase from Trichoderma viride |
|---|---|
| FullText URL | srfa_111_007_014.pdf |
| Author | Saito, Masaya| Inagaki, Kenji| |
| Abstract | L-Lysine α-oxidase (LysOX) from Trichoderma viride is a homodimeric flavoenzyme that catalyzes the oxidative deamination of L-Lysine to produce α-keto-ε-aminocaproate with ammonia and hydrogen per-oxide. LysOX inhibited the growth of cancer cells but showed relatively low toxicity for normal cells. The full-length cDNA consists of 2,119 bp, and encodes a long N-terminal propeptide composed of 77 resi-dues (Met1-Arg77) and the mature protein (Ala78-Ile617). The LysOX gene was heterologously expressed in Streptomyces lividans TK24 or Escherichia coli SoluBL21. The enzymatic properties of the purified recombinant LysOX, such as substrate specificity, kinetic parameters and thermal stability, are the same as those of the native LysOX. The LysOX precursor (prLysOX) expressed in E. coli shows weak enzymatic activity and is activated by proteolytic processing. The crystal structure of prLysOX revealed that the propeptide of prLysOX indirectly changes the active site structure to inhibit enzyme activity. Moreover, the crystal structures of LysOX and its L-Lysine complex revealed that the hydrogen bonding network formed by Asp212, Asp315 and Ala440 with two water molecules is responsible for the recogni-tion of the ε-amino group of L-Lysine. In addition, a narrow substrate-binding site and acidic surface at the active site entrance both contribute to strict substrate specificity. Mutational analysis demonstrated that Asp212 and Asp315 are essential for substrate recognition, and the D212A/D315A LysOX prefers aromatic amino acids. Furthermore, the structural basis of the substrate specificity change has also been revealed by the structural analysis of the D212A/D315A LysOX and its substrate complexes. |
| Keywords | L-lysine α-oxidase antitumor enzyme substrate recognition X-ray crystallography enzyme activity regulation |
| Publication Title | Scientific Reports of the Faculty of Agriculture, Okayama University |
| Published Date | 2022-02-01 |
| Volume | volume111 |
| Start Page | 7 |
| End Page | 14 |
| ISSN | 2186-7755 |
| language | Japanese |
| File Version | publisher |
| NAID | 120007190707 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Nakayama, Rie| Takaya, Yoichi| Nakamura, Kazufumi| Takemoto, Rika| Toh, Norihisa| Ito, Hiroshi| |
| Keywords | Congestion Heart failure Prognosis Shear wave elasticity |
| Published Date | 2022-01-17 |
| Publication Title | ESC Heart Failure |
| Volume | volume2022 |
| Publisher | Wiley Periodicals, Inc |
| ISSN | 2055-5822 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 The Authors. |
| File Version | publisher |
| PubMed ID | 35038790 |
| DOI | 10.1002/ehf2.13809 |
| Web of Science KeyUT | 000743285100001 |
| Related Url | isVersionOf https://doi.org/10.1002/ehf2.13809 |
| FullText URL | fulltext20220112-1.pdf |
|---|---|
| Author | Watanabe, Takaichi| Yasuhara, Yuka| Ono, Tsutomu| |
| Keywords | Microfluidics Multiple emulsion Poly(ionic liquid) Phase separation Non-equilibrium structure |
| Note | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Polymer Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsapm.1c01315| |
| Published Date | 2021-12-22 |
| Publication Title | ACS Applied Polymer Materials |
| Volume | volume4 |
| Issue | issue1 |
| Publisher | American Chemical Society (ACS) |
| Start Page | 348 |
| End Page | 356 |
| ISSN | 2637-6105 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | Copyright © 2021 American Chemical Society |
| File Version | author |
| DOI | 10.1021/acsapm.1c01315 |
| Web of Science KeyUT | 000736567900001 |
| Related Url | isVersionOf https://doi.org/10.1021/acsapm.1c01315 |
| FullText URL | fulltext20220104-5.pdf |
|---|---|
| Author | Zhang, Jingjing| Matsuo, Toshihiko| Hamasaki, Ichiro| Sato, Kazuhiro| |
| Keywords | strabismus esotropia exotropia diploid plant human quantitative trait locus (QTL) pooled genomic DNA chromosome single nucleotide polymorphism (SNP) whole exome sequencing |
| Published Date | 2021-12-27 |
| Publication Title | Life |
| Volume | volume12 |
| Issue | issue1 |
| Publisher | MDPI AG |
| Start Page | 41 |
| ISSN | 2075-1729 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
| File Version | publisher |
| DOI | 10.3390/life12010041 |
| Related Url | isVersionOf https://doi.org/10.3390/life12010041 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Okanoue, Shotaro| Iwamuro, Masaya| Tanaka, Takehiro| Satomi, Takuya| Hamada, Kenta| Sakae, Hiroyuki| Abe, Makoto| Kono, Yoshiyasu| Kanzaki, Hiromitsu| Kawano, Seiji| Kawahara, Yoshiro| Okada, Hiroyuki| |
| Keywords | gastrointestinal stromal tumors leiomyomas schwannomas tumor location |
| Published Date | 2021-10-08 |
| Publication Title | Medicine |
| Volume | volume100 |
| Issue | issue40 |
| Publisher | Lippincott Williams ＆ Wilkins |
| Start Page | e27520 |
| ISSN | 0025-7974 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021 the Author(s). |
| File Version | publisher |
| PubMed ID | 34622886 |
| DOI | 10.1097/MD.0000000000027520 |
| Web of Science KeyUT | 000704071200053 |
| Related Url | isVersionOf https://doi.org/10.1097/MD.0000000000027520 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Nakashima, Mitsutaka| Miyoshi, Toru| Ejiri, Kentaro| Kihara, Hajime| Hata, Yoshiki| Nagano, Toshihiko| Takaishi, Atsushi| Toda, Hironobu| Nanba, Seiji| Nakamura, Yoichi| Akagi, Satoshi| Sakuragi, Satoru| Minagawa, Taro| Kawai, Yusuke| Nishii, Nobuhiro| Fuke, Soichiro| Yoshikawa, Masaki| Nakamura, Kazufumi| Ito, Hiroshi| MUSCAT-HF Study Investigators| |
| Keywords | Estimated plasma volume Heart failure with preserved ejection fraction Luseogliflozin Sodium glucose co-transporter 2 inhibitors Voglibose |
| Published Date | 2021-11-03 |
| Publication Title | Esc Heart Failure |
| Publisher | Wiley Periodicals, Inc |
| ISSN | 2055-5822 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021 The Authors. |
| File Version | publisher |
| DOI | 10.1002/ehf2.13683 |
| Web of Science KeyUT | 000714002000001 |
| Related Url | isVersionOf https://doi.org/10.1002/ehf2.13683 |
| FullText URL | fulltext20211109-4.pdf |
|---|---|
| Author | Arimura, Yuki| Minato, Yusuke| Wada, Takayuki| Nakayama, Masaaki| Ryumon, Ayako| Hirata, Nao| Nakajima, Chie| Suzuki, Yasuhiko| Ato, Manabu| Kobayashi, Kazuo| Ohara, Naoko| Iida, Seiji| Ohara, Naoya| |
| Keywords | thymidylate synthase ThyX Mycobacterium BCG |
| Note | This is the peer reviewed version of the following article: [Arimura, Y, Minato, Y, Wada, T, Nakayama, M, Ryumon, A, Hirata, N, et al. Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG. Microbiol Immunol. 2021; 1– 5. https://doi.org/10.1111/1348-0421.12944], which has been published in final form at [https://doi.org/10.1111/1348-0421.12944]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages there of by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.| |
| Published Date | 2021-10-21 |
| Publication Title | Microbiology and Immunology |
| Volume | volume66 |
| Issue | issue1 |
| Publisher | Wiley |
| Start Page | 10 |
| End Page | 14 |
| ISSN | 0385-5600 |
| NCID | AA00738350 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021 The Societies and John Wiley & Sons Australia, Ltd |
| File Version | author |
| PubMed ID | 34546594 |
| DOI | 10.1111/1348-0421.12944 |
| Web of Science KeyUT | 000709482200001 |
| Related Url | isVersionOf https://doi.org/10.1111/1348-0421.12944 |
| JaLCDOI | 10.18926/AMO/62818 |
|---|---|
| FullText URL | 75_6_755.pdf |
| Author | Takagi, Kosei| Kuise, Takashi| Umeda, Yuzo| Yoshida, Ryuichi| Yoshida, Kazuhiro| Nagai, Yasuo| Noma, Kazuhiro| Tanabe, Shunsuke| Maeda, Naoaki| Yagi, Takahito| Fujiwara, Toshiyoshi| |
| Abstract | Approximately 4% of patients with esophageal cancer develop a second primary malignancy in the upper gastrointestinal trunk. However, hepatectomy following esophagectomy for esophageal cancer has rarely been reported. We report the case of a 70-year-old man who underwent an esophagectomy for esophageal cancer with retrosternal gastric tube reconstruction. Nine years later, he developed hepatocellular carcinoma with tumor thrombus involving the left portal vein, and was successfully treated with left hemihepatectomy. Special attention should be paid to avoiding incidental injury of the gastric tube as well as the right gastroepiploic artery during the hepatectomy. |
| Keywords | liver resection, esophagectomy, retrosternal gastric tube reconstruction |
| Amo Type | Case Report |
| Publication Title | Acta Medica Okayama |
| Published Date | 2021-12 |
| Volume | volume75 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 755 |
| End Page | 758 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2021 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 34955546 |
| Web of Science KeyUT | 000735319800004 |
| NAID | 120007180274 |
