岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024センター報告投稿要領7979ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024資料 : 2023年度7578ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024運営 : 2023年度1373ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024地域連携 : 2023年度1111ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024試験研究 : 2023年度79ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024教育 : 2023年度16ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024目次iiiiENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024序iiENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024表紙ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551152026裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551152026奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551152026公表学術論文等リスト 20251326ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551152026北海道東部の森林において林冠木の細根が土壌窒素動態に与える影響912ENMasatakaNakayamaCourse of Environmental Ecology Plants release mixtures of labile organic matter from their fine roots (root exudates) into the surrounding soil (rhizosphere). Partly due to the release of root exudates, microbial community structures and their activities within the rhizosphere differ significantly from those in other soil areas (bulk soil). Consequently, nutrient cycling processes, including nitrogen mineralization, are accelerated in the rhizosphere, facilitating nutrient acquisition by plants. This phenomenon, known as the rhizosphere effect, has been repeatedly reported in studies of herbaceous plants; however, the impact of canopy tree fine roots on soil nitrogen dynamics through the effect in forest ecosystems remains largely unknown. Here, I introduce our research investigating the root exudates and rhizosphere effects of the fine roots of canopy trees, Quercus crispula, and how these fine roots affect soil nitrogen dynamics. The quantity of root exudates varied daily rather than seasonally, with solar radiation having a strong and positive effect on the amounts. However, even after leaf fall, root exudation was observed. In the rhizosphere, specific bacterial communities were present regardless of season, while ectomycorrhizal fungal populations were higher than in the bulk soil only in summer. Extracellular enzymatic activity relating to nitrogen cycling was higher in the rhizosphere than in the bulk soil across seasons. Nitrogen uptake by the tree was likely lower in winter and spring, leading to labile nitrogen accumulation in the rhizosphere during these periods. On an annual basis, however, the impact of fine roots on apparent inorganic nitrogen dynamics was minor. These results suggest that the canopy tree, Q. crispula, accelerates soil nitrogen cycling through root exudation and rhizosphere effects, regardless of season, while the acceleration of the cycle and the utilization of available nitrogen are well-balanced annually, thereby avoiding unnecessary carbon investment.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551152026Evaluation of Branching Characteristics and Their Contribution to Yield in Everbearing Strawberry Cultivars under Forced Cultivation18ENMinoriHikawa-EndoKyushu Okinawa Region Agricultural Research Center, NAROKazuyoshiSoneKyushu Okinawa Region Agricultural Research Center, NAROMasamiMorishitaKyushu Okinawa Region Agricultural Research Center, NARO Enhancing continuous flowering in cultivated strawberries may result in insufficient photosynthetic products due to the lower limit of leaf number on each lateral shoot, leading to reduced yield and fruit quality. If strawberries could differentiate an appropriate number of tillers and allow each tiller to grow autonomously with sufficient leaf number on each lateral shoot, rather than flowering continuously on the main bud alone, plants could achieve high yields while preventing plant weakening and fruit quality deterioration. Therefore, this study evaluated branching characteristics of everbearing strawberry cultivars under forcing cultivation to identify cultivars with moderate tillering and moderately low continuous flowering. Pot experiments revealed that the number of tillers was high in ‘Summer Princess’ and ‘Miyazaki-natsuharuka’ but low in ‘Summer Berry’ and ‘Suzuakane’. This trend was independent of total number of lateral shoots, nodal position of first inflorescence, and the number of leaves on each lateral shoot, which serve as indicators of continuous flowering ability. Among seven tested cultivars, ‘DT17’ and ‘Miyazaki-natsuharuka’ showed intermediate values with 2.1 - 2.5 tillers per plant and 6.7 - 7.7 leaves on each lateral shoots. These cultivars showed yields of 747.0 - 1,028.5 g per plant under forcing cultivation, which were higher than other cultivars, along with consistent fruit quality. These results suggest that improving branching characteristics is a practical approach to enhancing fruit productivity in strawberries.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551152026目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551152026表紙ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025未利用資源の乳酸菌発酵による高付加価値化と化粧品原料への応用ENKanakoMATSUNAGAOkayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Studies on the functional roles of γδ T cells in the early phase of acute avian coccidiosisENLE VIET QUANGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Morphological/developmental characteristics and oxidative stress resilience of porcine oocytes derived from antral follicles with various diameters and classified by brilliant cresyl blue stainingENVAN NGOC PHONGGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025山口県育成小輪系ユリ切花の安定的周年供給体系の確立に関する研究ENYukoFUKUMITSUGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025A capsidless (+)RNA yadokarivirus hosted by a dsRNA victorivirus is infectious as particles, cDNA, and dsRNAENMUHAMMAD FADLIGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025イネおよびオオムギのイオン透過性アクアポリンにおける構造と透過機能の連関の分子生理学的研究ENShuntaroONOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Structural insights into chloroplast membrane remodeling: The role of VIPP1 oligomers in vascular plantsENSARAH WANJIRU GACHIEGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025A CRITICAL EVALUATION OF ABACA FARMERS’ HABITUS IN THE RISK ENVIRONMENT OF CATANDUANES, PHILIPPINESENNICCA AIRA AREVALO MARQUEZGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025ウメの青果流通期間拡大に向けた台湾ウメの果実発育・成熟特性の調査および鮮度保持技術の適用ENTomoakiKASHIWAMOTOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025The evolutionary contextualization and deep neural network modeling on fruit ripening responseENErikoKUWADAGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Investigation of novel genetic variants related to reproductive and productive traits in Vietnamese native buffaloENNGUYEN THANH THUYGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025夏季のミニトマト生産における暑熱対策に関する研究ENShujiKOZAIGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025SHORT AND CROOKED AWNを介したオオムギの芒形成機構の解析ENKokiNAKAMURAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025珪藻のシリカ被殻形成およびヒザラガイの磁鉄鉱歯形成に関わるタンパク質の機能解析ENKokiOKADAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025簡略化メタ統計的極値分布を用いた極値雨量のバイアス補正に関する研究ENKazukiSAKIKAWAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023センター報告投稿要領193193ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023資料 : 2020～2022年度185192ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023運営 : 2020～2022年度33184ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023地域連携 : 2020～2022年度2931ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023試験研究 : 2020～2022年度1528ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023教育 : 2020～2022年度113ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023目次ivvENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023序iiiiENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-874243-452023表紙ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025公表学術論文等リスト 20242738ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025アブラナ科植物黒斑細菌病の防除に向けた植物－病原細菌の相互作用の解明2125ENNanamiSakataCourse of Applied Plant Science Pseudomonas cannabina pv. alisalensis (Pcal), the causative agent of bacterial blight on cruciferous crops, is an economically important pathogen worldwide. We have conducted several studies on the interactions between plants and pathogenic bacteria to develop effective control strategies for this disease. Using forward and reverse genetics, we identified several virulence factors, including the type III secretion system, membrane transporters, transcriptional factors, and amino acid metabolism. Additionally, we emphasized the role of coronatine, a toxin produced by Pcal, which promotes stomatal reopening and suppresses salicylic acid accumulation in plants. We also examined plant defense mechanisms activated by one of the plant defense activators, acibenzolar-S-methyl (ASM). ASM enhanced stomatal-based defense, resulting in reduction of bacterial entry and disease development. Moreover, we explored innovative control strategies for bacterial disease and demonstrated that amino acids and cellulose nanofiber are efficient and environmentally friendly control strategies. These studies advance our understanding of plant-pathogen dynamics and offer promising, sustainable approaches for managing bacterial blight disease in cruciferous crops.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025遺伝子改変ラットを用いた弓状核キスペプチンニューロンの性腺刺激ホルモン放出ホルモンパルスジェネレーターとしての役割解明1120ENMayukoNagaeGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityYoshihisaUenoyamaGraduate School of Bioagricultural Sciences, Nagoya University Strategies for increasing reproductive performance are needed for domestic animals because for example the conception (pregnancy) rate has decreased in dairy cows around the world. Reproductive function is controlled by hormones released by the hypothalamus-pituitary-gonadal axis in mammals, including domestic animals. Of those hormones, tonic (pulsatile) gonadotropin-releasing hormone (GnRH) release is fundamentally important for gonadotropin release and gonadal activity. Therefore, uncovering the mechanism controlling GnRH pulses, that is GnRH pulse generator, is essential to improve reproductive technologies for domestic animals. The present review is focused on the indispensable role of arcuate nucleus (ARC) kisspeptin neurons (also known as KNDy neurons) as the GnRH pulse generator in mammals. First, we give a brief overview of studies on hypothalamic kisspeptin neurons throughout the past two decades. Second, we review studies that have provided direct evidence that ARC kisspeptin neurons serve as the GnRH pulse generator, with a special focus on our gene-modified rat models. Finally, we discuss the mechanism underlying GnRH pulse generation. The knowledge obtained from gene-modified rat models should be clinically important and could be adapted to new tools to improve reproductive performance in livestock by stimulating GnRH/gonadotropin pulses.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025Effects of dark respiration on dry matter production of various crop species110ENKuniyukiSaitohGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityTomohiroMurakamiGraduate School of Natural Science and Technology, Okayama UniversityYumiNakamuraSchool of Agriculture, Okayama UniversityMisaNishiboriSchool of Agriculture, Okayama UniversityYukiTakagoshiGraduate School of Natural Science and Technology, Okayama UniversityYoshihikoHiraiGraduate School of Environmental, Life, Natural Science and Technology, Okayama University Eleven crops were cultivated: maize, sunflower, soybean, groundnuts, sesame, kenaf, barley, wheat, rice, potato, and sweet potato. The crop growth rate (CGR) and specific dark-respiration rate (Rs) were measured, and growth efficiency GE =CGR/(CGR+R) (R, respiratory loss) was calculated. In each crop, whole-plant Rs reached a maximum in the earlier stages of growth, declined rapidly until the early reproductive growth, and remained almost constant during the ripening period. The Rs of leaves was higher than that of stems during the reproductive growth period, except for maize and potato. The Rs of storage organs was highest in the earlier stages, followed by a rapid decline to similar or lower values than those of leaves and stems during the ripening period. The GE in whole plant was higher than 60% in wheat, maize, barley, sunflower, rice, kenaf, sesame, but lower in soybean, sweet potato and groundnuts, and lowest in potato, which was affected by the higher respiratory loss. The GE in whole plant during the reproductive growth period was significantly lower, which we attributed to increased maintenance costs due to the increase of non-assimilative organs, and decrease in the dry weight of vegetative organs. A positive correlation was observed between the carbohydrate content of storage organs and GE, indicating that a crop with higher carbohydrate content in storage organs tended to have a higher GE. Crops with higher protein and crude fat content in storage organs tended to have lower GE. The GE over the growing season was low for kenaf, a fiber crop which contains high molecular weight compounds such as lignin and cellulose, and lower for sesame, groundnuts, and soybean, which contain high oil and protein and have high respiration costs for the synthesis of storage materials, suggesting that these higher respiration costs are related to lower dry matter production and hence lower yields.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551142025表紙ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Comparative research on antibacterial peptides, bacteriocins, produced by two strains of lactic acid bacteria, Leuconostoc mesenteroides subsp. mesenteroides 406 and 213M0, isolated from Mongolian traditional fermented milk, airagENHASIQIMUGEGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Water use properties contributing to the maintenance of plant biomass and grain production in NERICA upland rice subjected to dry soils ENNGUYEN THI THANH HIENGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Selection of non-fading lines for high quality cut-flower production in Dahlia variabilis ′Nessho′ by elucidating mechanisms of low temperature induced flower color fadingENMUTHAMIA EDNA KIRUMBAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024イチゴの周年生産に向けた花芽分化誘導処理と生産性に関する研究ENTakayoshiYANOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Crop protection activity and colonization behavior of Allorhizobium vitis VAR03-1, a biocontrol bacterium for grapevine crown gall diseaseENNIARSI MERRY HEMELDAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Study on the role of mineral elements in rice defense against herbivoresENDandy Ahamefula OsibeGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Identification of transporters involved in uptake of non-essential microelements in rice ENHengliangHUANGGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024A study on toxic mechanisms of SO2 in plant cells - Evaluation of the involvement of cytosolic acidification and cellular oxidation in SO2 toxicityENMAHDI MOZHGANIGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Studies on the role of organelle DNA degradation mediated by DPD1 nuclease in riceENMD. FARIDUL ISLAMGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024農業用水路トンネルの模型実験に対する数値解析の適用性に関する研究ENToshiyukiTAMOTOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Milk, udder skin, and fecal microbiota and their relationships with blood metabolites and milk composition in dairy herdsENAodaohuGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024Impact of Insulin-Like Growth Factor-1 on Bovine Follicular DynamicsENAhmad Farid RawanGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024シロイヌナズナのFZLタンパク質による葉緑体膜リモデリングの遺伝生理学的解析ENYuOGAWAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2024遊離N-グリカンの生理機能解析を目的としたエンド-β-N-アセチルグルコサミニダーゼ (ENGase) 欠損トマトの構築と植物ENGaseの糖転移活性に関する研究ENNaokoOKAMOTOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.American Chemical SocietyActa Medica Okayama2470-13439192024Superstructure of Fe5–xGeTe2 Determined by Te K-Edge Extended X-ray Absorption Fine Structure and Te Kα X-ray Fluorescence Holography2128721297ENRitsukoEguchiResearch Institute for Interdisciplinary Science, Okayama UniversityHalubaiSekharDepartment of Physical Science and Technology, Nagoya Institute of TechnologyKojiKimuraDepartment of Physical Science and Technology, Nagoya Institute of TechnologyHirokazuMasaiDepartment of Materials and Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)NaohisaHappoGraduate School of Information Sciences, Hiroshima City UniversityMitsukiIkedaResearch Institute for Interdisciplinary Science, Okayama UniversityYukiYamamotoResearch Institute for Interdisciplinary Science, Okayama UniversityMasakiUtsumiResearch Institute for Interdisciplinary Science, Okayama UniversityHidenoriGotoResearch Institute for Interdisciplinary Science, Okayama UniversityYasuhiroTakabayashiDepartment of Physical Science and Technology, Nagoya Institute of TechnologyHirooTajiriJapan Synchrotron Radiation Research Institute (JASRI)KoichiHayashiDepartment of Physical Science and Technology, Nagoya Institute of TechnologyYoshihiroKubozonoResearch Institute for Interdisciplinary Science, Okayama UniversityThe local structure of the two-dimensional van der Waals material, Fe5–xGeTe2, which exhibits unique structural/magnetic phase transitions, was investigated by Te K-edge extended X-ray absorption fine structure (EXAFS) and Te Kα X-ray fluorescence holography (XFH) over a wide temperature range. The formation of a trimer of Te atoms at low temperatures has been fully explored using these methods. An increase in the Te–Fe distance at approximately 150 K was suggested by EXAFS and presumably indicates the formation of a Te trimer. Moreover, XFH displayed clear atomic images of Te atoms. Additionally, the distance between the Te atoms shortened, as confirmed from the atomic images reconstructed from XFH, indicating the formation of a trimer of Te atoms, i.e., a charge-ordered (3⎯⎯√×3⎯⎯√)𝑅30◦ superstructure. Furthermore, Te Kα XFH provided unambiguous atomic images of Fe atoms occupying the Fe1 site; the images were not clearly observed in the Ge Kα XFH that was previously reported because of the low occupancy of Fe and Ge atoms. In this study, EXAFS and XFH clearly showed the local structure around the Te atom; in particular, the formation of Te trimers caused by charge-ordered phase transitions was clearly confirmed. The charge-ordered phase transition is fully discussed based on the structural variation at low temperatures, as established from EXAFS and XFH.No potential conflict of interest relevant to this article was reported.岡山大学教育学部国語研究会Acta Medica Okayama2189-5414382024文学／国語教育とアニマルウェルフェア ――「フランドン農学校の豚」・「大造じいさんとガン」・国語教科書教材について――119ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020センター報告投稿要領6363ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020資料 : 2019年度5962ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020運営 : 2019年度1558ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020地域連携 : 2019年度1314ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020試験研究 : 2019年度711ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020教育 : 2019年度16ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020目次iiiiENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020序iiENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742422020表紙ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024公表学術論文等リスト 20236173ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024接ぎ木による開花誘導のアブラナ科作物の育種および採種への利用5559ENKoMotokiCourse of Applied Plant ScienceGrafting-induced flowering has the potential to be applied to plant breeding and seed production, to shorten the time needed for floral induction, and to expand the environment in which seed production is possible. However, it is difficult to induce flowering by grafting in some crop species, which currently limits the use of this technique to specific plant species. We have established a technique for inducing flowering in cabbage (Brassica oleracea L.) by grafting, which had hitherto been considered difficult, by clarifying the causes of different flowering responses caused by various rootstocks. This article introduces the key factors for the floral induction of cabbage by grafting and its potential application to the breeding and seed production of cruciferous crops. It was observed that cabbage grafted onto B. oleracea rootstocks did not flower at all, while cabbage grafted onto some accessions of Raphanus sativus rootstocks did flower. Furthermore, the ability of R. sativus to induce flowering of grafted cabbage varied even within the species. Immunoblotting analysis of the FLOWERING LOCUS T (FT) protein, a main component of florigen, revealed that floral induction was quantitatively correlated with the level of accumulated FT protein in the grafted scion. It was concluded that increasing the total amount of FT protein produced in the rootstock is important for the stable floral induction of the grafted cabbage, and this can be accomplished by increasing FT transcription and the leaf area of the rootstock. Field cultivation experiments with cabbage progenies obtained by the grafting method indicated the direct applicability of this method to breeding and seed production in cruciferous crops.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024四倍体コムギが保有する新規早生遺伝子の探索およびその効率的遺伝解析法の開発4954ENKazusaNishimuraCourse of Applied Plant ScienceWheat (Triticum spp.) is one of the world's three major cereals and a staple food for more than 35% of the world's population, and while nearly doubling crop yields is essential to meet the caloric demands of a growing population by 2050, current rates of wheat yield growth are insufficient to meet this demand. Therefore, it is important to promote the improvement of wheat varieties and to increase yields in a stable manner by utilizing all available genetic resources. Here I would like to introduce some research that has been conducted in this regard, using tetraploid wheat (Triticum turgidum L.) as a genetic resource in the search for genes related to flowering time and the research related to the streamlined genetic analysis technique.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024Instant estimation of rice yield using ground-based RGB images and its potential applicability to UAV4148ENYuTanakaGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityTomoyaWatanabeGraduate School of Mathematics, Kyushu UniversityKeisukeKatsuraGraduate School of Agriculture, Tokyo University of Agriculture and TechnologyYasuhiroTsujimotoJapan International Research Center for Agricultural SciencesToshiyukiTakaiJapan International Research Center for Agricultural SciencesTakashi Sonam TashiTanakaFaculty of Biological Sciences, Gifu UniversityFaculty of Biological Sciences, Gifu UniversityKensukeKawamuraJapan International Research Center for Agricultural SciencesHirokiSaitoTropical Agriculture Research Front, Japan International Research Center for Agricultural SciencesKokiHommaGraduate School of Agricultural Science, Tohoku UniversitySalifou GoubeMairouaAfrica Rice Center （AfricaRice）KokouAhouantonAfrica Rice Center （AfricaRice）AliIbrahimAfrica Rice Center （AfricaRice）, Regional Station for the SahelKalimuthuSenthilkumarAfrica Rice Center （AfricaRice）Vimal KumarSemwalAfrica Rice Center （AfricaRice）, Nigeria StationEduardo Jose GraterolMatuteLatin American Fund for Irrigated Rice - The Alliance of Bioversity International and CIATEdgarCorredorLatin American Fund for Irrigated Rice - The Alliance of Bioversity International and CIATRaafatEl-NamakyRice Research and Training Center, Field Crops Research InstituteNorvieManigbasPhilippine Rice Research Institute （PhilRice）Eduardo Jimmy P.QuilangPhilippine Rice Research Institute （PhilRice）YuIwahashiGraduate School of Agriculture, Kyoto UniversityKotaNakajimaGraduate School of Agriculture, Kyoto UniversityEisukeTakeuchiGraduate School of Agriculture, Kyoto UniversityKazukiSaitoJapan International Research Center for Agricultural SciencesRice (Oryza sativa L.) is one of the most important cereals, which provides 20% of the world’s food energy. However, its productivity is poorly assessed especially in the global South. Here, we provide a first study to perform a deep learning-based approach for instantaneously estimating rice yield using RGB images. During ripening stage and at harvest, over 22,000 digital images were captured vertically downwards over the rice canopy from a distance of 0.8 to 0.9m at 4,820 harvesting plots having the yield of 0.1 to 16.1 t ha-1 across six countries in Africa and Japan. A convolutional neural network (CNN) applied to these data at harvest predicted 68% variation in yield with a relative root mean square error (rRMSE) of 0.22. Even when the resolution of images was reduced (from 0.2 to 3.2cm pixel-1 of ground sampling distance), the model could predict 57% variation in yield, implying that this approach can be scaled by use of unmanned aerial vehicles. Our work offers low-cost, hands-on, and rapid approach for high throughput phenotyping, and can lead to impact assessment of productivity-enhancing interventions, detection of fields where these are needed to sustainably increase crop production.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024異なる施肥条件下における水稲品種の窒素利用効率 ―日本晴とタカナリの比較―3339ENKuniyukiSaitohThe Graduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityYusukeDannoThe Graduate School of Natural Science and Technology, Okayama UniversityThe rice cultivar Nipponbare and the high-yielding cultivar Takanari were cultivated in field trials for three years from 2003, and in pot trials (1/2,000a) for two years from 2004. In the field trials, the following three levels of fertilizer were applied：“0N” without fertilizer, “1N” with the standard amount(8kgN 10a–1), and “2N” with twice the standard amount (16kgN 10a–1). In the pot trial, three levels of fertilizer were applied：“0N” without fertilizer, “1.5N” with 1.5times the standard amount (1.5gN pot–1), and “3N” with three times the standard amount (3gN pot–1). In the field trials, yields were higher in Takanari (538 to 843g m–2) than in Nihonbare (423 to 577g m–2), and the increase in yield with fertilizer application was also larger in Takanari. This was related to the larger sink capacity of Takanari and the smaller decrease in the percentage of filled grain with larger sink capacity. The dry matter weight and nitrogen uptake at the panicle initiation stage were higher in the plots with higher fertilizer application, but the differences between the cultivars were small. Dry matter weight and nitrogen uptake at harvest time were higher in Takanari, and nitrogen use efficiency and sink production efficiency were also higher in Takanari, but the differences in nitrogen use efficiency between cultivars became smaller with increasing fertilizer application. The nitrogen use efficiency for dry matter production also decreased with increasing fertilizer application, and was higher in 2005 in Takanari. The leaf photosynthetic rate of Takanari was higher than that of Nipponbare in the pot experiment. The difference in leaf photosynthetic rate was related to the nitrogen use efficiency (photosynthetic rate / leaf nitrogen content), and the difference in leaf nitrogen content between cultivars was small. The nitrogen use efficiency for dry matter was highest in the “0N” and decreased with increasing fertilizer application, and was higher in Takanari than in Nipponbare. This was presumably related to the higher nitrogen use efficiency of photosynthesis. It was found that fertilizer application decreased nitrogen use efficiency and sink production efficiency, but yield increased with increasing sink capacity, and that differences in nitrogen use efficiency among cultivars were related to the amount of nitrogen absorbed up to the panicle initiation stage and sink production efficiency. In order to improve the efficiency of fertilizer application, it is desirable to increase nitrogen absorption, which is expressed as multiplying the number of days to panicle initiation and the rate of nitrogen absorption, and to select cultivars with higher sink production efficiency.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024Analytical studies on high-yielding characteristics of US soybean cv. ‘UA4805’ in comparison with Japanese cv. ‘Akimaro’2532ENSultanzada MohammadMaroufGraduate School of Environmental and Life ScienceYuHasegawaSchool of AgricultureRyutaManabeSchool of AgricultureKuniyukiSaitohGraduate School of Environmental and Life ScienceField experiments were conducted in 2020 and 2021 at the Field Science Center of Okayama Univ. (34°41’ N, 133°55’ E). Two Soybean cultivars ‘UA4805’ and ‘Akimaro’ were sown with two planting densities, 12.5plants m−2 (sparse, 80×10cm) and 25plants m−2 (dense, 80×5cm)on May 25 (early), June 29 (normal), and Aug. 3 (late) in 2020, and 80 and 30cm row-width, and 12.5 and 25 plant m−2 in 2021 on June 23. Seed yield was higher in ‘UA4805’ than in ‘Akimaro’ in 2020 and 2021. The later the sowing time, the higher the seeds/stem ratio. Both cultivars showed higher dry matter in dense planting. Dry matter was higher in ‘Akimaro’, while seed yield was lower than ‘UA4805’. In contrast, ‘UA4805’ showed lower dry matter with higher seed yield. The numbers of nodes, pods, and seeds were higher in ‘UA4805’ resulting in the higher seed yield. Lodging score is larger in ‘Akimaro’ especially in dense planting. The seeds/stem ratio is much higher in ‘UA4805’ than ‘Akimaro’ across 2 densities, 3 sowing times and 2 row width. Pods setting ratio was nearly two times higher in ‘UA4805’ compared to ‘Akimaro’. The greater seed yield of ‘UA4805’ compared to ‘Akimaro’ was due to the higher pod setting ratio, seeds/stem ratio, and lower lodging score, nevertheless the dry matter was larger in ‘Akimaro’. If late sowing is applied, higher planting density is recommended for better seed yield. Narrow row is an effective way to improve seed yield in soybean.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024水稲における窒素利用効率の品種間差異1724ENKuniyukiSaitohThe Graduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityYoshifumiIwameThe Graduate School of Natural Science and Technology, Okayama UniversityMasahikoMaekawaInstitute of Plant Science and Resources （IPSR）, Okayama UniversityKazuyoshiTakedaInstitute of Plant Science and Resources（IPSR）, Okayama UniversityWe investigated the effects of fertilizer-free and fertilizer-applied cultivation on growth, yield and nitrogen (N) utilization of rice cultivars in our Kurashiki paddy fields (Institute of Plant Science and Resources, Okayama Univ.), which have been cultivated without fertilizer since 1970, and also in our Okayama paddy fields, which are conventionally cultivated. In 2001, the cultivars Nipponbare (NIP) and Nourin 18 (N18) were cultivated in the Kurashiki fields, with a “0N plot” (no fertilizer application), a “1N plot” (standard fertilizer application), and a “2N plot” (double fertilizer application). In 2002, five cultivars were grown without fertilizer in the Kurashiki fields, and 51cultivars were tested in 0N and 1N plots in the Okayama fields. Yield (2001) in the Kurashiki fields was higher in the 0N plot for N18 (379g m–2), which had a higher number of spikelets per m2, than NIP (300 g m–2), while in the 1N and 2N plots it was higher for NIP, which had a higher percentage of ripening, and N18 had high yield potential even without fertilizer application, but low fertilizer tolerance. The differences in yield were related to N-uptake (NU), and the differences in N use efficiency (NUE, yield/NU) between cultivars were small. The pot experiment showed that the yield of 0N plot was higher for N18 than NIP grown in Kurashiki soil because of the higher number of spikelets per hill, and the yield in the Okayama soil was higher than that in the Kurashiki soil. Long-term non-fertilized soils are of poor soil fertility, which also decreases the NUE, and the NUE of N18 is higher than that of NIP under isolated conditions. The difference in yields is closely related to sink capacity (SC). In 2002, yields in the Kurashiki fields were highest in Takanari (TAK, 494g m–2) and lowest in NIP (350g m–2), and differences in yields were closely related to SC. NUE was highest in TAK (68.6) and lowest in Akebono (48.1). TAK had high NUE and high sink production efficiency (SPE, SC/NU), while N18 had low NUE but high SC due to higher NU, ensuring high yield even under unfertilized cultivation. Yields in the 0N and 1N plots cultivated in 2002 varied between 244–631g m–2 and 199–769g m–2, respectively. A close positive correlation was observed between yield and SC, and between NU and SC, suggesting that the SC through NU is involved in determining yield. A positive correlation was also observed between NUE and yield. It was found that yield increased with an increase in NUE, and that NUE decreased although yield increased with fertilizer application. Through selection of cultivars with high SPE, it is expected that it will be possible to breed low-input, high-yielding cultivars with high NUE in the future.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024水稲NERICA品種の農業形質に関する遺伝的要因1116ENAsamiTomitaCourse of Applied Plant ScienceYuiAkimotoCourse of Applied Plant ScienceGenetic factors of yield related traits in lowland New Rice for Africa variety (NERICA) using different nitrogen condition and QTL to increase rice yield in lowland NERICAs were clarified. Indica Group rice variety (Oryza sativa L.) IR64 increased panicle number, culm weight and panicle weight, according to nitrogen concentration. The tendency is especially remarkable under low nitrogen concentration. NERICA‒L‒19, which is derived from a cross between IR64 and TOG5681 (Oryza glaberrima S.), showed similar values for panicle weight and culm weight, and it showed higher plant height, panicle weight and culm weight and lower harvest index than those of IR64 under all the concentrations. QTL analysis for the agronomic traits were performed using hybrid population derived from a cross between IR64 and NERICA‒L‒19. A total of 22 QTLs were detected on chromosomes (chrs.) 1 (3 QTLs), 2 (2), 4 (2), 5 (5), 6 (1), 7 (1), 8 (6) and 10 (2). Among them, 4 regions at 34.1‒36.6Mbp of chr. 1, at 23.8‒27.8Mbp of chr. 5 and at 4.8Mbp and 20.3‒28.4Mbp of chr. 8 showed several QTLs’ peaks overlapped. The regions of chr. 5 and 4.8Mbp of chr. 8 increased panicle weight by IR64 allele. Those of chr. 1 and 20.3-28.4Mbp of chr. 8 increased plant height and harvest index, respectively, by NERICA‒L‒19 allele. These results indicated that the larger panicle type of lowland NERICA is suitable under low nitrogen condition. Also, the plant type was controlled by the combination of 4 QTLs’ regions on chr. 1 and short arm of chr. 8 originated from O. glaberrima, and those of chr. 5 and long arm of chr. 8 from O. sativa Indica Group.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024Effect of seed cold PEG-priming and subsequent long storage on germination, growth and flowering of Eustoma grandiflorum（Raf.）Shinn ʻExe Lavenderʼ710ENThao ThuPhanGraduate School of Environmental and Life Science, Okayama UniversityKeigoFukushimaHiroshima Prefectural Technology Research Institute, Agricultural Technology Research CenterYoshiyukiTanakaGraduate School of Environmental and Life Science, Okayama UniversityKen-ichiroYasubaGraduate School of Environmental and Life Science, Okayama UniversityYuichiYoshidaGraduate School of Environmental and Life Science, Okayama UniversityTanjuroGotoGraduate School of Environmental and Life Science, Okayama UniversityCold wet treatment is frequently applied to Eustoma seeds to enhance the bolting rate of plants grown under high temperatures. Our previous study indicated that cold PEG‒primed Eustoma seeds could maintain their germination rate and bolting rate even after being re‒dried for 30days and grown under high temperatures. The present study aimed to investigate whether prolonged storage after cold PEG‒priming affect the germination, growth, and flowering of Eustoma ‘Exe Lavender’ seedling. Seeds were initially cold‒primed with water or PEG‒6000 at ‒1.5 MPa for 5 weeks at 10°C in the dark and were then subjected to re‒drying and storage for 30, 60, 90 and 360days at 10°C. After 360days of storage, cold PEG‒primed seeds germinated earlier and more effectively than cold hydro‒primed seeds. Compared to the results after 30days of storage, plants grown from 360days exhibited similar bolting rate, days to bolting, bolting node and flowering rate, cut flower length and number of flower node. These results suggest that ‘Exe Lavender’ seeds can germinate and develop well even after 360days of storage and under high temperature conditions when subjected to cold PEG‒6000 treatment.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024過剰発現プロファイリングADOPT法を用いたS.cerevisiaeのワイン醸造用ブドウ果汁への適応性評価16ENHisaoMoriyaCourse of Agrochemical BioscienceChiyuki Kohata OnoCourse of Agrochemical BioscienceThe authors have recently developed the overexpression profiling ADOPT method. In the ADOPT method, yeast strains overexpressing most of the genes in the budding yeast Saccharomyces cerevisiae genome are mixed and competitively cultured, and the genes overexpressed in the enriched strains are systematically identified. Furthermore, the identified genes can be used to identify bottleneck factors that are necessary but lacking for growth of S. cerevisiae under given conditions. In our previous studies, we have identified bottlenecks in artificially created stress environments in the laboratory, but in this study, we used grape juice for winemaking as an example to see if industrial bottlenecks can be identified. ADOPT experiments with sulfite-added grape juice used in conventional winemaking resulted in a strong enrichment of strains overexpressing the sulfite pump SSU1 and its transcription factor FZF1. Since enhancement of SSU1 function is known to occur in wine yeast acclimation, ADOPT was also shown to be useful in the search for industrial bottlenecks. On the other hand, no genes were strongly enriched by ADOPT in grape juice without sulfite addition, suggesting that grape juice is a balanced medium with few bottlenecks for S. cerevisiae growth.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551132024表紙ENNo potential conflict of interest relevant to this article was reported.Society for Reproduction and DevelopmentActa Medica Okayama0916-88186962023Effects of insulin-like growth factor-1 on the mRNA expression of estradiol receptors, steroidogenic enzymes, and steroid production in bovine follicles337346ENAhmad FaridRawanLaboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama UniversityHikmatullahLangarLaboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama UniversityMahoMunetomoLaboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama UniversityYukiYamamotoLaboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama UniversityKoheiKawanoLaboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama UniversityKojiKimuraLaboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama UniversityInsulin-like growth factor-1 (IGF-1) plays a crucial role in follicular growth and stimulates steroid hormone production in bovine follicles. Steroid hormones are synthesized through the actions of steroidogenic enzymes, specifically STAR, CYP11A1, HSD3B, and CYP19A1 in both theca cells (TCs) and granulosa cells (GCs), under the influence of gonadotropins. Particularly, estradiol 17 beta (E2) assumes a central role in follicular development and selection by activating estrogen receptors beta (ESR2) in GCs. We assessed ESR2 mRNA expression in GCs of developing follicles and investigated the impact of IGF-1 on the mRNA expression of ESR2, CYP19A1, FSHR, and LHCGR, STAR, CYP11A1, and HSD17B in cultured GCs and TCs, respectively. Additionally, we assessed the influence of IGF-1 on androstenedione (A4), progesterone (P4), and testosterone (T) production in TCs. Small-sized follicles (< 6 mm) exhibited the highest levels of ESR2 mRNA expression, whereas medium-sized follicles (7-8 mm) displayed higher levels than large-sized follicles (>= 9 mm) (P < 0.05). IGF-1 increased the mRNA expression of ESR2, CYP19A1, and FSHR in GCs of follicles of both sizes, except for FSHR mRNA in medium-sized follicles (P < 0.05). IGF-1 significantly elevated mRNA expression of LHCGR, STAR, CYP11A1, and CYP17B in TCs of small-and medium-sized follicles (P < 0.05). Moreover, IGF-1 augmented the production of A4 and P4 but had no impact on T production in TCs of small-and medium-sized follicles. Taken together, our findings indicate that IGF-1 upregulates steroidogenic enzymes and steroid hormone production, underscoring the crucial role of IGF-1 in follicle development and selection.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2023Assessment of factors affecting the motility and viability of frozen-thawed bull spermatozoaENNGUYEN THANH HAIGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2023Characterization of physiological functions of dihydroxyacetone in ArabidopsisENMaoxiangZhaoGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005農業技術の普及と導入行動に関する経営発展論的分析ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005抽水雑草キショウブの植生護岸構成種への活用に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005植物病原細菌Ralstonia solanacearumのhrpB依存性遺伝子の網羅的単離と機能解析ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005シロイヌナズナにおけるPot1様タンパク質の解析ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2005虚血性不整脈における容積感受性陰イオンチャネルの役割 -新規容積感受性陰イオンチャネル阻害薬を用いた検証-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2023Analysis of genetic diversity and population structure in Cambodian melon landraces using molecular markersENPERVIN MST NAZNINGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2023Studies on self-assembly of anionic cyclic lipodepsipeptide surfactin for its applicationENSatohiroYANAGISAWAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2023過剰発現によりストレス環境への耐性を向上させる酵母遺伝子集団の性質に関する研究ENNozomuSAEKIGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2023Maoecrystal Vの全合成に向けたヘテロ環構築に関する研究ENShunkiKATAYAMAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023公表学術論文等リスト 20222336ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023鳥類におけるアルギニンバソトシン神経の性差1922ENNoboruSaitoCourse of Applied Animal ScienceArginine vasotocin (AVT) is one of neurohypophysial peptides in birds and is well known both as an antidiuretic and oxytocic hormone. AVT is produced in the hypothalamus and mainly synthesized in magnocellular neurons, supraoptic nucleus (SON) and paraventricular nucleus (PVN). Recent, immunocytochemistry and in situ hybridization results indicate that AVT is also synthesized in parvocellular neurons corresponding to mammalian stria terminalis (nST). The AVT neurons in nST in the male are more numerous than those in the female. Therefore, these data suggest that there is sexual dimorphism in the distribution of AVT neurons. Several experiments involving electrical lesion, testosterone implantation, and the detection of the immediately-early gene expression in birds expressing copulatory behavior suggested that nST and nucleus praeopticus medialis (POM) were related to the reproductive behavior. When male embryo was treated with estradiol, the distribution of AVT neurons assumes the same distribution as found in the female, and reproductive behavior was abolished, suggesting that AVT in the brain has an important role in reproductive behavior.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023Streptomyces属放線菌由来の高基質特異性l-グルタミン酸オキシダーゼに関する研究1318ENNatsumeNakayamaCourse of Agrochemical BioscienceK.Inagakil-glutamate oxidase (LGOX) from Streptomyces sp. is a heterohexameric flavin enzyme that catalyzes the oxidative deamination of l-glutamate to form α-ketoglutarate with ammonia and hydrogen peroxide. LGOX shows strict substrate specificity for l-Glu. In addition, it is highly thermostable and pH stable. Because of these properties, LGOX is currently used as a biosensor for the trace determination of l-Glu in the food industry and clinical laboratories. The full-length cDNA is 2103 bp and is encoded by a single polypeptide chain consisting of 701 residues including subunits α-γ-β. The LGOX gene was heterologously expressed in Escherichia coli JM109. The LGOX precursor expressed in E. coli is a homodimer with weak enzymatic activity and becomes a heterohexamer upon activation by protease treatment. X-ray crystallography and docking studies of purified recombinant LGOX suggest that the Arg305 residue is a key residue for substrate recognition. Mutant analysis showed that Arg305 is essential for substrate recognition, as the activity toward l-Glu was greatly reduced and substrate specificity was changed in some enzymes. The functional analysis of R305E-LGOX, which is an l-Arg oxidase, revealed that R305E-LGOX can be used as a enzyme biosensor for l-Arg.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023Effect of seed cold PEG–priming concentration and period followed by re–drying on germination, growth and flowering of Eustoma grandiflorum （Raf.） Shinn ʻExe Lavenderʼ712ENThao Thu PhanGraduate School of Environmental and Life Science, Okayama UniversityKeigoFukushimaHiroshima Prefectural Technology Research Institute, Agricultural Technology Research CenterYoshiyukiTanakaGraduate School of Environmental and Life Science, Okayama UniversityKen-ichiroYasubaGraduate School of Environmental and Life Science, Okayama UniversityYuichiYoshidaGraduate School of Environmental and Life Science, Okayama UniversityTanjuroGotoGraduate School of Environmental and Life Science, Okayama UniversityThis study aimed to investigate whether seed cold priming by PEG‒6000, followed by re‒drying would affect the germination, growth, and flowering of Eustoma ‘Exe Lavender’ raised seedling under high temperatures. Seeds of control treatment were not treated with cold‒priming or re‒drying. In experiment 1, seeds were cold‒primed with water and PEG‒6000 at ‒0.75 and ‒1.5 MPa for 5 weeks at 10°C in the dark and then subjected to re‒dried and storage for 30 days at 10°C. The results showed that cold‒primed seeds germinated earlier than those of the control. Compared to water treatment, plants grown from PEG‒6000 treatments and re‒drying showed similar germination rate, bolting rate, days to bolting, bolting node and flowering rate. In experiment 2, cold priming periods by water and ‒1.5 MPa PEG‒6000 were investigated for 4, 5, 6, 7, and 8 weeks. ‘Exe Lavender’ seeds treated with cold water and in the dark for longer than 5 weeks increased their percentage of radicle emergence and decreased germination rate after 30 days of re‒drying and storage. Meanwhile, those treated with cold-PEG did not protrude the radicle even for 8 weeks of priming and maintained the germination rate after re‒drying and storage. Days to bolting and days to flowering tended to decrease when the priming period decreased. The results suggest that the seed cold priming treatment by ‒1.5 MPa PEG‒6000 and subsequent re‒drying improved germination rate and maintained bolting rate and flowering rate in Eustoma compared to cold priming treatment by water raised seedling under high temperature conditions.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023Comparison of LEDs, Fluorescent Lamps and Incandescent Bulbs for Long-day Treatment of Forcing Strawberries as Affected by Respective Color Temperature16ENYuichiYoshidaGraduate School of Environmental and Life Science, Okayama UniversityAtsushiHanadaGraduate School of Environmental and Life Science, Okayama UniversityKeitaOogumaFaculty of Agriculture, Okayama UniversityKentaYoshidaJapan Agency for Marine-Earth Science and TechnologyTanjuroGotoGraduate School of Environmental and Life Science, Okayama UniversityYoshiyukiTanakaGraduate School of Agriculture, Kyoto UniversityKen-ichiroYasubaGraduate School of Environmental and Life Science, Okayama UniversityLong day (LD) treatment is frequently applied for horticultural crops, including strawberry, and sufficient effect similar to 4 to 6hours of day time elongation in the evening can be achieved by 1 to 3hours of night break in the mid night. Incandescent lamps (IC) have long been an important light source, but the general incandescent lamps are out of production and have been replaced with LED or florescent lamps (FL). Thus, we compared light sources and their color temperature (2,800K, warm white similar to IC; 6,500K, daylight) in different lighting programs. The effectiveness of 3 and 4cycles of alternate intermittent lighting, 30min on/30min off, were equal to and larger than 2hours of night break, respectively. Measured intensity of illumination (lux) at the canopy surface by 2,800K LED (6.9W) and FL (12W) was 1.50 and 0.95times as large as IC (54W), but leaf growth under LED and FL was similar to and less vigorous than IC, respectively. On the other hand, intensity values of illumination by 6,500K LED (6.9W) and FL (12W) were both 1.20times as large as 2,800K ones, but 6,500K light sources were much less effective compared to 2,800K ones. Daylight type LED and FL which have higher luminance efficiency but emit less red light compared to warm white ones, are not suitable as alternative light sources for IC.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551122023表紙ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019センター報告投稿要領6767ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019資料 : 2018年度6366ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019運営 : 2018年度2355ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019地域連携 : 2018年度2121ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019モモ‘紅清水’における赤肉果の音響振動法による非破壊判別技術の検討913ENFumioFukudaKenHiranoKunihisaMorinagaNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019試験研究 : 2018年度919ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019教育 : 2018年度16ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019序iiEN岡山大学農学部附属山陽圏フィールド科学センターNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742412019表紙ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Genetic polymorphism of β-casein variants in Jersey cows and nutritional assessment of A1 and A2 caseins in miceENMinNuoGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Lactation-long changes in milk microbiota, milk composition, and metabolic profiles of Holstein and Jersey cows and the environmental microbiota of their barnsENGATHINJI Peter KiiruGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Analysis of heterosis based on barley genomeENSAKKOURAREEJGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Diverse roles of a siderophore in a plant-growth promoting methylotroph, Methylobacterium aquaticum strain 22AENPATRICK OTIENO JUMAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Molecular characterization of quantitative trait loci controlling element accumulation in sorghum grainsENFIONA WACERA WAHINYAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Bacterial and fungal microbiota involved in the anaerobic storage of foragesENJianjianHouGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022カキ果実の輸出および貯蔵技術に関する研究ENTakahiroFurutaGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022エディブルコーティングによる青果物の品質保持効果とその作用機構に関する研究ENNatsukiHiraGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Molecular mechanisms for plastic sex expression in hexaploid persimmonENKanaeMasudaGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Relationship between classification of Rhizoctonia solani species complex and pathogenicity on Arabidopsis thalianaENMai Mohsen Ahmed Abdelghany AhmedGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022Studies on the NB-LRR-encoding genes conferring susceptibility to organophosphate pesticides and leaf greenness in sorghumENZihuanJingGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022The taxonomic and phenotypic diversity of Methylobacterium speciesENOLA ALESSAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022The mechanism of malate-induced stomatal closure in Arabidopsis thalianaENYoshiharuMimataGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022ケルセチン配糖体腸内細菌代謝物のアセトアルデヒドに対する保護作用とその分子機構ENTakumiMyojinGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2022PLP依存性L-メチオニン代謝関連酵素の構造機能解析と応用ENAtsushiOkawaGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-7755111 Supplement2022Proceedings of the International Symposium on Animal Bioscience 2021ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022裏表紙・英文目次1ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022公表学術論文等リスト 20212742ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022外来水生植物キショウブの繁殖特性と適切な管理法2125ENYoshitakaNakashimaCourse of Applied Plant Science, The Faculty of Agriculture, Okayama UniversityTo establish a proposal for a guiding principle supporting appropriate management of Iris pseudacorus L., which had been used as an important vegetation bank species until the Invasive Alien Species Act, this study investigated propaga-tion characteristics. The seed in which seed coat cracked germinated earlier, and it was shown that their cumulative germination percentage was high even under 30 °C constant conditions. When the fresh weight of the rhizome cut pieces in the spring became greater than 15 g, the individual plant flowering rate increased gradually. Individuals of more than 30 g achieved a flowering rate higher than 76 %. The flow-ering rate was highest for individual plants that had two green leaves, but it became progressively lower for increas-ing numbers of green leaves：non-flowering individuals grew better than flowering individuals. In the 0 cm height scape-cutting treatment, no significant difference was found in the numbers of flowers and fruits the next year. In the 5 cm height top-cutting treatment, the flowers and fruits in the next year were significantly fewer. No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022Silene 属植物が保持する性染色体ビッグデータと性操作する菌1520ENNaokoFujitaCourse of Applied Plant Science, The Faculty of Agriculture, Okayama UniversitySilene latifolia (Caryophyllaceae) is a dioecious plant that has long been used for study on sex chromosomes in plants. The advantage but also disadvantage of S. latifolia as a model system is the size of the Y chromosome that contains an extremely large male-specific region (approx. > 500 Mb). This feature implies that the evolutionary history of sex chromo-somes remains in the S. latifolia Y chromosome, while the size makes analyses esoteric. Another advantage is that plants in the genus Silene show variation in reproductive systems; most are gynodioecy (females and hermaphrodites), which is thought of as an evolutionary status before establishment of dioecy (males and females), with a few hermaphrodites and dioecy, suggesting that the genus Silene may represent an epitome of the sex chromosome evolution. Microbotryum is a biotrophic fungi, whose infection causes masculinization of the female flower, as if the fungus acts as the Y chromo-some. Though the underlying molecular mechanisms remain unknown, recent high-throughput sequence technologies provide many candidate genes for sex determination in plants and sex conversion by the fungus. In this article, I review and introduce studies of the Y chromosome in S. latifolia plant, the evolution of sex chromosomes in the genus Silene, the masculinization of female flowers caused by a fungus infection, and a virus vector that can be used for genetic analysis of the key genes involved in these processes.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022糸状菌Trichoderma viride由来の抗腫瘍性酵素L-リシン α-オキシダーゼに関する研究714ENMasayaSaitoCourse of Agrochemical BioscienceKenjiInagakiL-Lysine α-oxidase (LysOX) from Trichoderma viride is a homodimeric flavoenzyme that catalyzes the oxidative deamination of L-Lysine to produce α-keto-ε-aminocaproate with ammonia and hydrogen per-oxide. LysOX inhibited the growth of cancer cells but showed relatively low toxicity for normal cells. The full-length cDNA consists of 2,119 bp, and encodes a long N-terminal propeptide composed of 77 resi-dues (Met1-Arg77) and the mature protein (Ala78-Ile617). The LysOX gene was heterologously expressed in Streptomyces lividans TK24 or Escherichia coli SoluBL21. The enzymatic properties of the purified recombinant LysOX, such as substrate specificity, kinetic parameters and thermal stability, are the same as those of the native LysOX. The LysOX precursor (prLysOX) expressed in E. coli shows weak enzymatic activity and is activated by proteolytic processing. The crystal structure of prLysOX revealed that the propeptide of prLysOX indirectly changes the active site structure to inhibit enzyme activity. Moreover, the crystal structures of LysOX and its L-Lysine complex revealed that the hydrogen bonding network formed by Asp212, Asp315 and Ala440 with two water molecules is responsible for the recogni-tion of the ε-amino group of L-Lysine. In addition, a narrow substrate-binding site and acidic surface at the active site entrance both contribute to strict substrate specificity. Mutational analysis demonstrated that Asp212 and Asp315 are essential for substrate recognition, and the D212A/D315A LysOX prefers aromatic amino acids. Furthermore, the structural basis of the substrate specificity change has also been revealed by the structural analysis of the D212A/D315A LysOX and its substrate complexes. No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022Effect of Re-Drying and Storage after 10°C Wet Treatment on Germination and Growth of Five Eustoma grandiflorum (Raf.) Shinn Cultivars16ENThao Thu PhanGraduate School of Environmental and Life ScienceKeigo FukushimaHiroshima Prefectural Technology Research Institute, Agricultural Technology Research CenterYoshiyukiTanakaGraduate School of Environmental and Life ScienceKen-ichiroYasubaGraduate School of Environmental and Life ScienceYuichiYoshidaGraduate School of Environmental and Life ScienceTanjuroGotoGraduate School of Environmental and Life ScienceIn Eustoma (Eustoma grandiflorum (Raf.) Shinn) cultivation in southwestern Japan, cold imbibition treatment of seeds is important to accelerate bolting and flowering of seedlings grown in the summer season. In order to facilitate the handling of cold imbibed seeds, re-drying treatment (RDT) after wet treatment at 10°C for 35 days in dark conditions (WT10°C) is considered to be an essential technique. The investigation of five Eustoma cultivars ‘Exe Lavender’, ‘Orb Snow’, ‘Philia Lavender’ , ‘Dure Lavender’ and ‘Exe Light Pink’ was to find the germinability and the growth characteristics of Eustoma when re-drying and storage at 10°C for 30 days were applied immedietely after ending the WT10°C. The results showed that germination and growth responses differed among Eustoma cultivars. RDT seeds and non- RDT seeds of ‘Exe Lavender’, ‘Orb Snow’ and ‘Philia Lavender’ had a similar germination rate. However, germination rate of RDT seeds in ‘Dure Lavender’ and ‘Exe Light Pink’ was lower than that of non-RDT seeds. There was no significant difference in cut-flower quality grown from RDT and non-RDT among ‘Exe Lavender’, ‘Orb Snow’ and ‘Dure Lavender’. However, bolting and flowering rate of RDT in ‘Dure Lavender’ and ‘Exe Light Pink’ were lower than those of non-RDT. This suggested that it might not be useful to apply RDT after the cold-wet treatment of Eustoma seed due to lower germination or bolting rates in some cultivars. Further investigation of cold-wet exposure and dehydrating conditions is required to establish RDT procedures for Eustoma cultivars.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551112022表紙ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Production optimization, and structural and functional characterization of exopolysaccharides from Pediococcus pentosaceus FFC003 and Enterococcus faecium AK1247ENJunliangZhaoGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Study on the intestinal conditions of chicken orally administrated with Lactobacillus acidophilus strain L-55 under the parasite infection or virus vaccinationENPHAM HOANG SON HUNGGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021腫瘍内18F-fluorodeoxyglucose 最大取込み細胞の細胞生物学的特性に関する研究ENSachiSugitaGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Identification and validation of salt-tolerant QTLs relating to grain yield and yield components using IR64 CSSLs in the genetic background of Koshihikari and their backcross progeniesENNGUYEN SAO MAIGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021ダッチアイリス切り花の開花不良要因の解明と貯蔵前処理による品質保持技術の開発ENNorikoToyoharaGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Developmental process and factors affecting fasciation of strawberry floral organsENNGUYEN THI CAMlGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Molecular and biological properties of diverse fungal and plant partitivirusesENTELENGECH Paul KipkemboiGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Cell-physiological study of salt tolerance in PoaceaeENTRAN THI HUONG SENGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021シアリルTn抗原結合アフィニティーカラムによる緑藻ミル（Codium fragile）レクチンの簡易精製法の確立と一次構造解析ENRyotaTakeuchiGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021岡山県のモモ栽培における気象変動が生理障害発生に及ぼす影響の把握と対策技術の開発ENYuichiroFujiiGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021PHYTOCLOCK 1 confer extreme early flowering in wheatENNUMANUL HAQUE GULAM KIBRIA MDNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021大豆用高速畝立て播種機の開発ENKentaShigematsuGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021高アミノ酸摂取における腸内細菌叢の変化と難消化性多糖の共摂取が宿主に及ぼす影響ENRyodaiTakagakiGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Microbial fermentation and quality preservation of agro-industrial wet by-productsENAjmalWaliGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Gut microbiota of rats and mice fed different sources of dietary protein and fatENSouliphoneSivixayGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Alteration of immune responses in bovine endometrial cells under hyperthermia conditionsENShunsukeSakaiGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021メロン・キュウリ遺伝資源の多様性及びその有効利用に関する遺伝学的研究ENGentaroShigitaGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2021Experimental introduction of mycoviruses into phytopathogenic filamentous fungi and their biologic characterizationENShahiSabitreeGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155813312021新型コロナウイルスを正しく知る6267ENTetsuyaMizutaniResearch and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and TechnologyNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551102021裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551102021奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551102021公表学術論文等リスト 20201531ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551102021花色の安定化と切り花の延命に関する研究713ENYoshikuniKitamuraGraduate School of Environmental and Life Science, Okayama University My research interest is in the physiology and ecology of ornamental plants. Here, I introduce my research subjects on the stabilization of flower color and cut flower longevity. Seasonal color change of dahlia (Dahlia variabilis) is studied as practical study for improving the stable dahlia cut flower production during autumn and winter. The results obtained from pigment analysis and clone selection are presented. Research on cut flower longevity is presented from two aspects. Firstly, study on the structure of conductive tissues that contribute to water transport is presented. Secondly, study on the floral organs that contribute to transpiration from cut flowers is presented.No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551102021遺伝子比較解析による珪藻類特異的なSET ドメインタンパク質ファミリーの同定16ENMichikoNemotoGraduate School of Environmental and Life Science, Okayama University The silica cell walls of diatoms, which exhibit species-spe-cific micro- and nano- patterned structures are promising candidates for applications in nanotechnology. Previous studies revealed a number of silica cell wall-associated proteins involved in silica formation. However, molecular biological analyses toward understanding of diatom cell wall formation have been mostly limited to model diatom species and general silica formation process in diatoms is still incompletely understood. In this study, to gain a compre-hensive insight into diatom silica biomineralization, tran-scriptome data of three diatom species, Nitzschia palea, Achnanthes kuwaitensis and Pseudoleyanella lunata, were newly developed. The reads obtained from RNA sequencing were assembled into 31,946, 60,767 and 38,314 unique transcripts for N. palea, A. kuwaitensis and P. lunata, respectively. In order to identify the diatom-specific genes, three transcriptome data sets developed in this study and the protein-coding gene sets of five genome-sequenced diatoms were compared. The proteins shared only by eight diatom species that are predicted to possess an endoplasmic reticulum (ER)-targeting signal peptide were selected for further analyses. These include proteins showing homology to silicanin-1, a recently reported diatom-specific protein involved in silica formation, as well as a number of SET domain proteins. The SET domain proteins might be novel diatom-specific family of methyltransferases that may reg-ulate the function of silica formation related proteins or long chain polyamines. The genes encoding the diatom-specific SET domain proteins identified in this study, which were shown to respond to silicon were suggested to be implicated in silica biomineralization. No potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551102021目次ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部Acta Medica Okayama2186-77551102021表紙ENNo potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama2051-817X8222020Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditionse14640ENShunsukeSakaiLaboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama UniversityToshimitsuHatabuLaboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama UniversityYukiYamamotoLaboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama UniversityKojiKimuraLaboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama UniversityAfter parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (M Phi s) to the subepithelial stroma. These attracted M Phi s are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5 degrees C) and HS conditions (40.5 degrees C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p < .05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p < .05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p < .05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of M Phi s; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p < .05) attracted the M Phi s when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of M Phi s in the endometrium during the summer.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Morphological and molecular characterization of melon landraces (Cucumis melo L.) from central and southern VietnamENDuong Thanh ThuyNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Assessment of milk and gut microbiota of lactating Jersey cows in relation to milk quality and animal health managementENNguyen Dang QuiGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Study on genes associated with reproductive and other economically important traits in Asian original cattle breedsENLe Nu Anh ThuGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Study on genetic diversity and characteristics of Kazakhstan original horsesENNguyen Ba TrungGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Study on the immune responses in the chickens with Eimeria parasite infection or lactic acid bacteria administration: Toward the application of Lactobacillus acidophilus L-55 as an anticoccidial agentENHo Thi DungGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Studies on flowering control of Eustoma grandiflorum seedling raised under high temperatures by using re–drying storage of cold imbibed seed and intermittent low temperature storageENPhan Thu ThaoGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Identification and characterization of effector candidate protein genes of Rhizoctonia solani, a causal agent of sheath blight disease using Brachypodium distachyonENSobhy Salama Hussein AbdelsalamGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Lanthanide-dependent methylotrophic pathway in Methylobacterium aquaticum strain 22AENPatcha YanriratGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020The role of hormonal signaling in rice defense against herbivores with focus on ethylene functionENMujiono KadisGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Physiological and Molecular Characterization of Zinc Uptake in RiceENShengHuangGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山大学 資源植物科学研究所 野生植物グループActa Medica OkayamaアキノエノコログサENNo potential conflict of interest relevant to this article was reported.岡山大学 資源植物科学研究所 野生植物グループActa Medica OkayamaヒルガオENNo potential conflict of interest relevant to this article was reported.岡山大学 資源植物科学研究所 野生植物グループActa Medica OkayamaゴボウENNo potential conflict of interest relevant to this article was reported.岡山大学 資源植物科学研究所 野生植物グループActa Medica OkayamaシロバナシナガワハギENNo potential conflict of interest relevant to this article was reported.岡山大学 資源植物科学研究所 野生植物グループActa Medica OkayamaコバナキジムシロENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020The relationship between uterine, milk, and environmental microbiota in postpartum dairy cowsENTHI THUONGNGUYENOkayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020農業雇用労働者の雇用管理と能力開発に関する研究ENSatokoIibaOkayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020小卵胞由来ブタ卵母細胞の成熟能及び初期発生能に関する研究ENChiyukiOnoGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Study on the mechanism of dynamic changes in the number of ciliated and non-ciliated cells in the bovine oviductENSayakaItoGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020The transcriptional regulation of fruit ripening by low temperatureENOSCAR WITEREMITALOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Interactions between two naturally co-infecting mycoviruses in the chestnut blight fungus Cryphonectria parasiticaENAULIAANNISA'Graduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Mapping and functional characterization of two genes involved in root development of riceENEnYuGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020粘膜関連リンパ組織リンパ腫転座1 (Malt1) のプロテアーゼ活性による炎症反応の制御とその機序に関する研究ENYoshikiNakamuraGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2020Pre-pollination reproductive isolation allowing the species coexistence of the genus Arisaema (Araceae)ENTetsuyaMatsumotoGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742402018奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742402018センター報告投稿要領 6767ENNo potential conflict of interest relevant to this article was reported.