塗抹染色及び過酸化酵素反応による穿液刺の細胞学的研究 第3編 各種疾患々者腹水及び胸水の細胞学的研究

As the results of cytological investigations cf ascites and thoracic fluid in various diseases, the author recognized the cellular elements and the staining findings characteristic to each disease; and in the application of these for the diagnosis of various diseases the author obtained the following findings that may be used as the criteria for the differential diagnosis of these diseases: 1. In the case of liver cirrhosis, serosal cells, lymphocytes and small phagocytes increase in number, and neutrophils also incrsase slightly; and of them serosal cells increase in all cases. The cell body of phagocyte, having many vacuoles and marked meshwork, in general is easily destroyed. Moreover, one half of the cases are consisted of signet-ring cells derived from phagocytes, and in some instances mitotic picture can be observed. 2. In the case of nephrosis, lymphocytes and small phagocytes increase; and in general small cells occupy the major portion of the cells. As for phagocytes, the degree of degeneration in both the nucleus and cell body is high, and hence the cell is easily destructible 3. In tuberculous disease lymphocytes and small phagocytes increase markedly, and these small cells occupy the major portion. The break-down of phagocytes is rare, and in rare instances signet-ring cells derived from phagocytes and mitotic picture can be observed. 4. In the case of heart disease lymphocytes and small phagocytes are numerous. 5. The characteristics of tumorous ascites and thoracic fluid are the appearance of tumor cells, but even in the absence of tumor cells still there exists a peculiar characteristic of its own in other kinds of cells. Namely, an increase in serosal cells, the appearance of abnormal serosal cells (many nuclei, giant nucleoli, vacuole formation, deep stain of the cell body, and indistinctness of nuclear boundary), an increase of neutrophils in some cases, and appearance of signet-ring cells, and mitotic picture. These are thought to be indirect signs of tumorous ascites and thoracic fluid, and in later examinations tumor cells have been detected in the majority of them.