Leptospira icterohaemorrhagiaeの電子顕微鏡的形態の研究 第2編 各種処理法に依る形態の研究

In order to investigate the morphlogical structure of Laptospira icterohaemorrhagiae grown up in Korthof media, the anther observed the gradual disintegration of the organism theated with distilled water, Na-desoxycholate, hydrochloric acid and Antiformin, and by freezing-thawing method. And the following results were obtained. 1) To disintegrate organism, the application of distilled water was most effective by its powerful swelling and busting of the organism, then the freezing-thawing method came in second. Hydrochloric acid had a effect to increase the density of Cytoplasm for electron beam. Na-desoxycholate and Antiformin were not so effective to disintegrate organism. 2) The slime layer covering surface of the organism supposedly corresponded to the slime layer of bacteria, and assumed that was not belonged to the structure of organism. 3) Occasionally it was observed fine fibers on the field treated with Na-desoxycholate. Hawever, it was impossible to decide the origin of this fibers. 4) The presence of covering membrane could be clarly proved by the tratment with distilled water on the organism. 5) The cytoplasm tended to show granule-like aspect after various treatment. 6) It could not demonstrate the presence of nuclear structure.