骨髓の組織呼吸並に解糖作用に関する研究 第2編 実験的瀉血貧血家兎骨髄に於ける呼吸解糖作用並に同血清が正常家兎骨髄酸素消費に及ぼす影響に就て第2編 実験的瀉血貧血家兎骨髄に於ける呼吸解糖作用並に同血清が正常家兎骨髄酸素消費に及ぼす影響に就て

By determining periodically the O(2)-consumption and the anaerobic glycolysis in the bone marrow of the rabbits depleted successively 4 times the rate of 10 c.c. /kg., with the slicing and the homogenate methods mentioned in the previous report, and by tracing the effects of the sera of these depleted rabbits on the O(2)-consumption in the bone marrow of normal rabbits, the authors have obtained the following results: 1. As regards the rate of O2-consumption in the case where the slicing method had been used or that in the homogenate method, the rates in both cases tended to increase 24 hours after a single depletion, and it later showed a continuous rise. Whereas in those rabbits subjected to four successive depletions, the O(2)-consumption reached the highest point 24 hours afterwards, and on cessation of depletion and following the gradual recovery of anemia, the O(2)-consumption decreased concomitantly; and 9 days after the cessation of depletion the rate returned more or less to normal. 2. The rate of O(2)-consumption paralleled with the percentage of immature cells of the erythroblastic system as well as of erythroblasts in the bone marrow and with the increase of mitotic figures. 3. A slight increase of the anaerobic glycolysis Q(M)(N(2)) could be observed only during the depleting periods in the case where either of the two methods had been employed; and the periodic fluctuations of Q(M)(N(2)) tended to be similar to those of the O(2)-consumption. The rate, however, returned to normal 3 days after the cessation of depletion; and later lowering slightly, it finally returned to normal. 4. A parallel relationship existed between the lowering of respiratory and glycolytic ration (Q(M)(N(2))/Q(O(2))) and the percentage of the myeloid cells in the bone marrow. 5. Some substances promoting the O(2)-consumption of the bone marrow of normal rabbits were found to exist in the serum of the depleted rabbits previously mentioned; and the time of the appearance or the disappearance of these substances as well as their effects seemed to coincide with the amounts of O(2)-consumption of the bone marrow itself. 6. These substances found in the above-mentioned serum were found to be so heatresistant that they could not be destroyed even when heated at the temperature of 100°C for 30 minutes.