start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=2
article-no=
start-page=267
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250122
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Abnormal Expression of Tubular SGLT2 and GULT2 in Diabetes Model Mice with Malocclusion-Induced Hyperglycemia
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: A relationship between malocclusion and the promotion of diabetes has been suggested. In hyperglycemia, the expression of sodium-glucose cotransporter 2 (SGLT2) and the facilitative glucose transporter 2 (GLUT2) is upregulated in proximal tubular cells, leading to an increase in renal glucose reabsorption. The present study aimed to investigate whether malocclusion contributes to diabetic exacerbation. Methods: Streptozotocin (STZ)-induced diabetic mice with malocclusion due to cutting molars were investigated based on increased blood glucose levels. PCR and immunohistochemical analyses were performed on diabetic mice kidneys to investigate the expression of SGLT2 and GLUT2. Results: Animal experiments were performed using 32 mice for 21 days. The time to reach a diabetic condition in STZ-administered mice was shorter with malocclusion than without malocclusion. The increase and mean blood glucose levels in STZ-administered mice were steeper and higher with malocclusion than without malocclusion. Urea albumin, BUN, and CRE levels were higher in diabetic mice with malocclusion than in diabetic mice without. Immunoreaction with anti-SGLT2 and anti-GLUT2 in the renal tissue of STZ-administered mice was stronger with malocclusion than without malocclusion. The amounts of SGLT2 and GLUT2 mRNA in the renal tissue in STZ-administered mice were higher with malocclusion than without malocclusion. The amounts of TNF-a and IL-6 mRNA in the large intestinal tissue in STZ-administered mice were higher with malocclusion than without malocclusion. Conclusions: Our results indicate that malocclusion accelerates the tubular expression of SGLT2 and GLUT2 under hyperglycemia. Malocclusion may be a diabetes-exacerbating factor with increased poor glycemic control due to shortened occlusion time resulting from swallowing food without chewing.
en-copyright=
kn-copyright=

en-aut-name=KajiwaraKoichiro
en-aut-sei=Kajiwara
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TamaokiSachio
en-aut-sei=Tamaoki
en-aut-mei=Sachio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SawaYoshihiko
en-aut-sei=Sawa
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Department of Oral Growth & Development, Fukuoka Dental College
kn-affil=

affil-num=2
en-affil=Department of Oral Growth & Development, Fukuoka Dental College
kn-affil=

affil-num=3
en-affil=Department of Oral Function & Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=malocclusion
kn-keyword=malocclusion
en-keyword= hyperglycemia
kn-keyword= hyperglycemia
en-keyword= SGLT2
kn-keyword= SGLT2
en-keyword= GLUT2
kn-keyword= GLUT2
END

start-ver=1.4
cd-journal=joma
no-vol=26
cd-vols=
no-issue=1
article-no=
start-page=38
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250124
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Exacerbation of diabetes due to F. Nucleatum LPS-induced SGLT2 overexpression in the renal proximal tubular epithelial cells
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background Diabetes treatments by the control of sodium-glucose cotransporter 2 (SGLT2) is commonly conducted while there are still uncertainties about the mechanisms for the SGLT2 overexpression in kidneys with diabetes. Previously, we have reported that glomeruli and proximal tubules with diabetic nephropathy express toll-like receptor TLR2/4, and that the TLR ligand lipopolysaccharide (LPS) of periodontal pathogens have caused nephropathy in diabetic model mice. Recently, many researchers suggested that the periodontal pathogenic bacteria Fusobacterium (F.) nucleatum has the TLR4-associated strong activator of the colorectal inflammation and cancer. The present study aimed to investigate the possibility of F. nucleatum as an exacerbation factor of diabetes through the renal SGLT2 induction.<br>
Methods The induction of the SGLT2 by F. nucleatum LPS (Fn-LPS) were investigated in the streptozotocin-induced diabetic mouse renal tissue and cultured renal proximal epithelial cells. The changes of blood glucose levels and survival curves in diabetic mice with Fn-LPS were analyzed. The Fn-LPS-induced SGLT2 production in the diabetic mouse renal tissue and in the cultured proximal epithelial cells was examined by ELISA, quantitative RT-PCR, and immunohistochemical analysis.<br>
Results The SGLT2 expression in the cultured mouse tubular epithelial cells was significantly increased by TNF- or co-culture with Fn-LPS-supplemented J774.1 cells. The period to reach diabetic condition was significantly shorter in Fn-LPS-administered diabetic mice than in diabetic mice. All Fn-LPS-administered-diabetic mice reached humane endpoints during the healthy period of all of the mice administered Fn-LPS only. The promotion of the SGLT2 expression at the inner lumen of proximal tubules were stronger in the Fn-LPS-administered-diabetic mice than in diabetic mice. The renal tissue SGLT2 mRNA amounts and the number of renal proximal tubules with overexpressed SGLT2 in the lumen were more in the Fn-LPS-administered-diabetic mice than in diabetic mice.<br>
Conclusions This study suggests that F. nucleatum causes the promotion of diabetes through the overexpression of SGLT2 in proximal tubules under the diabetic condition. Periodontitis with F. nucleatum may be a diabetic exacerbating factor.
en-copyright=
kn-copyright=

en-aut-name=SekiAiko
en-aut-sei=Seki
en-aut-mei=Aiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KajiwaraKoichiro
en-aut-sei=Kajiwara
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TeramachiJumpei
en-aut-sei=Teramachi
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=EgusaMasahiko
en-aut-sei=Egusa
en-aut-mei=Masahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MiyawakiTakuya
en-aut-sei=Miyawaki
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SawaYoshihiko
en-aut-sei=Sawa
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
kn-affil=

affil-num=2
en-affil=Department of Oral Growth & Development, Fukuoka Dental College
kn-affil=

affil-num=3
en-affil=Department of Oral Function & Anatomy, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
kn-affil=

affil-num=4
en-affil=Department of Dental Anesthesiology & Special Care Dentistry, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
kn-affil=

affil-num=5
en-affil=Department of Dental Anesthesiology & Special Care Dentistry, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
kn-affil=

affil-num=6
en-affil=Department of Oral Function & Anatomy, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
kn-affil=
en-keyword=F. Nucleatum
kn-keyword=F. Nucleatum
en-keyword=Diabetic exacerbation
kn-keyword=Diabetic exacerbation
en-keyword=Diabetic nephropathy
kn-keyword=Diabetic nephropathy
en-keyword=SGLT2
kn-keyword=SGLT2
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240925
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=胆汁酸塩をco-formerとしたbrick dust AntiY5R の共非晶質化と溶解性改善
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=AIKAWAShohei
en-aut-sei=AIKAWA
en-aut-mei=Shohei
kn-aut-name=相川昇平
kn-aut-sei=相川
kn-aut-mei=昇平
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240925
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=クロスカルメロースナトリウムの塩基性環境下における高吸水性ゲル化を利用した新規徐放化システムの開発
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=GOMIMasato
en-aut-sei=GOMI
en-aut-mei=Masato
kn-aut-name=五味真人
kn-aut-sei=五味
kn-aut-mei=真人
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=5
article-no=
start-page=972
end-page=984
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=202411
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A randomized, open-label, clinical trial examined the effects of canagliflozin on albuminuria and eGFR decline using an individual pre-intervention eGFR slope
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Demonstrating drug efficacy in slowing kidney disease progression requires large clinical trials when targeting participants with an early stage of chronic kidney disease (CKD). In this randomized, parallel-group, open-labeled trial (CANPIONE study), we assessed the effect of the sodium-glucose cotransporter 2 (SGLT2) inhibitor canagliflozin using the individual’s change in estimated glomerular filtration rate (eGFR) slope before (pre-intervention slope) and during treatment (chronic slope). We randomly assigned (1:1) participants with type 2 diabetes, urinary albumin-to-creatinine ratio (UACR) of 50 to under 300 mg/g, and an eGFR of at least 45 ml/min/1.73m2 to receive canagliflozin or guideline-recommended treatment except for SGLT2 inhibitors (control). The first and second primary outcomes were the geometric mean percentage change from baseline in UACR and the change in eGFR slope, respectively. Of 98 randomized participants, 96 received at least one study treatment. The least-squares mean change from baseline in log-transformed geometric mean UACR was significantly greater in the canagliflozin group than the control group (between group-difference, −30.8% (95% confidence interval −42.6 to −16.8). The between-group difference (canagliflozin group – control group) of change in eGFR slope (chronic – pre-intervention) was 4.4 (1.6 to 7.3) ml/min/1.73 m2 per year, which was more pronounced in participants with faster eGFR decline. In summary, canagliflozin reduced albuminuria and the participant-specific natural course of eGFR decline in participants with type 2 diabetes and microalbuminuria. Thus, the CANPIONE study suggests that the within-individual change in eGFR slope may be a novel approach to determine the kidney protective potential of new therapies in early stages of CKD.
en-copyright=
kn-copyright=

en-aut-name=MiyamotoSatoshi
en-aut-sei=Miyamoto
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HeerspinkHiddo J.L.
en-aut-sei=Heerspink
en-aut-mei=Hiddo J.L.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=de ZeeuwDick
en-aut-sei=de Zeeuw
en-aut-mei=Dick
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SakamotoKota
en-aut-sei=Sakamoto
en-aut-mei=Kota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshidaMichihiro
en-aut-sei=Yoshida
en-aut-mei=Michihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ToyodaMasao
en-aut-sei=Toyoda
en-aut-mei=Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SuzukiDaisuke
en-aut-sei=Suzuki
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=HatanakaTakashi
en-aut-sei=Hatanaka
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NakamuraTohru
en-aut-sei=Nakamura
en-aut-mei=Tohru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KameiShinji
en-aut-sei=Kamei
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=MuraoSatoshi
en-aut-sei=Murao
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=HidaKazuyuki
en-aut-sei=Hida
en-aut-mei=Kazuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=AndoShinichiro
en-aut-sei=Ando
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=AkaiHiroaki
en-aut-sei=Akai
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=TakahashiYasushi
en-aut-sei=Takahashi
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=KitadaMunehiro
en-aut-sei=Kitada
en-aut-mei=Munehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=SuganoHisashi
en-aut-sei=Sugano
en-aut-mei=Hisashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=NunoueTomokazu
en-aut-sei=Nunoue
en-aut-mei=Tomokazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=NakamuraAkihiko
en-aut-sei=Nakamura
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=SasakiMotofumi
en-aut-sei=Sasaki
en-aut-mei=Motofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=NakatouTatsuaki
en-aut-sei=Nakatou
en-aut-mei=Tatsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=FujimotoKei
en-aut-sei=Fujimoto
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

en-aut-name=KawanamiDaiji
en-aut-sei=Kawanami
en-aut-mei=Daiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=

en-aut-name=WadaTakashi
en-aut-sei=Wada
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=24
ORCID=

en-aut-name=MiyatakeNobuyuki
en-aut-sei=Miyatake
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=25
ORCID=

en-aut-name=KuramotoHiromi
en-aut-sei=Kuramoto
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=26
ORCID=

en-aut-name=ShikataKenichi
en-aut-sei=Shikata
en-aut-mei=Kenichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=27
ORCID=

affil-num=1
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen
kn-affil=

affil-num=3
en-affil=Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen
kn-affil=

affil-num=4
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=5
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=6
en-affil=Division of Nephrology, Endocrinology and Metabolism, Department of Internal Medicine, Tokai University School of Medicine
kn-affil=

affil-num=7
en-affil=Suzuki Diadetes Clinic
kn-affil=

affil-num=8
en-affil=Department of Diabetes and Endocrinology, National Hospital Organization Fukuyama Medical Center
kn-affil=

affil-num=9
en-affil=Diabetes Internal Medicine, Sumitomo Besshi Hospital
kn-affil=

affil-num=10
en-affil=Department of Diabetic Medicine, Kurashiki Central Hospital
kn-affil=

affil-num=11
en-affil=Department of Diabetes and Endocrinology, Takamatsu Hospital
kn-affil=

affil-num=12
en-affil=Department of Diabetology and Metabolism, National Hospital Organization Okayama Medical Center
kn-affil=

affil-num=13
en-affil=Department of Internal Medicine Diabetic Center, Okayama City Hospital
kn-affil=

affil-num=14
en-affil=Division of Diabetes and Metabolism, Faculty of Medicine, Tohoku Medical and Pharmaceutical University
kn-affil=

affil-num=15
en-affil=Department of Diabetes, Ochiai General Hospital
kn-affil=

affil-num=16
en-affil=Department of Diabetology and Endocrinology, Kanazawa Medical University
kn-affil=

affil-num=17
en-affil=Department of Diabetes and Endocrinology, Kochi Health Sciences Center
kn-affil=

affil-num=18
en-affil=Nunoue Clinic
kn-affil=

affil-num=19
en-affil=Internal Medicine, Osafune Clinic
kn-affil=

affil-num=20
en-affil=Department of Diabetes and Endocrinology, Matsue City Hospital
kn-affil=

affil-num=21
en-affil=Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=22
en-affil=Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, The Jikei University Kashiwa Hospital
kn-affil=

affil-num=23
en-affil=Department of Endocrinology and Diabetes, Fukuoka University School of Medicine
kn-affil=

affil-num=24
en-affil=Department of Nephrology and Laboratory Medicine, Graduate School of Medical Sciences, Kanazawa University
kn-affil=

affil-num=25
en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=26
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=27
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=
en-keyword=canagliflozin
kn-keyword=canagliflozin
en-keyword=CANPIONE study
kn-keyword=CANPIONE study
en-keyword=chronic kidney disease microalbuminuria
kn-keyword=chronic kidney disease microalbuminuria
en-keyword=preintervention eGFR slope
kn-keyword=preintervention eGFR slope
en-keyword=sodium-glucose cotransporter 2 inhibitor
kn-keyword=sodium-glucose cotransporter 2 inhibitor
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=1
article-no=
start-page=16337
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240716
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of dapagliflozin on myoglobin efflux from cardiomyocyte during myocardial ischemia/reperfusion in anesthetized rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It has been suggested that sodium-glucose cotransporter 2 (SGLT2) inhibitors have cardioprotective effects during myocardial ischemia/reperfusion (I/R) independent of glucose-lowering action. However, the effects of SGLT2 inhibitors on structural damage to cardiomyocytes in the ischemic region during I/R remain unknown. We applied a microdialysis technique to the heart of anesthetized rats and investigated the effects of an SGLT2 inhibitor, dapagliflozin, on myocardial interstitial myoglobin levels in the ischemic region during coronary occlusion followed by reperfusion. Dapagliflozin was administered systemically (40 mu g/body iv) or locally via a dialysis probe (100 mu M and 1 mM) 30 min before coronary occlusion. In the vehicle group, coronary occlusion increased the dialysate myoglobin concentration in the ischemic region. Reperfusion further increased the dialysate myoglobin concentration. Intravenous administration of dapagliflozin reduced dialysate myoglobin concentration during ischemia and at 0-15 min after reperfusion, but local administration (100 mu M and 1 mM) did not. Therefore, acute systemic administration of dapagliflozin prior to ischemia has cardioprotective effects on structural damage during I/R.
en-copyright=
kn-copyright=

en-aut-name=HayashidaTomohiro
en-aut-sei=Hayashida
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KurokoYosuke
en-aut-sei=Kuroko
en-aut-mei=Yosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ShimizuShuji
en-aut-sei=Shimizu
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AkiyamaTsuyoshi
en-aut-sei=Akiyama
en-aut-mei=Tsuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SuezawaTakanori
en-aut-sei=Suezawa
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KiokaYukio
en-aut-sei=Kioka
en-aut-mei=Yukio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KotaniYasuhiro
en-aut-sei=Kotani
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ShishidoToshiaki
en-aut-sei=Shishido
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KasaharaShingo
en-aut-sei=Kasahara
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences and Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Cardiac Physiology, National Cerebral and Cardiovascular Center
kn-affil=

affil-num=5
en-affil=Department of Cardiovascular Surgery, Fukuyama City Hospital
kn-affil=

affil-num=6
en-affil=Department of Cardiovascular Surgery, Fukuyama City Hospital
kn-affil=

affil-num=7
en-affil=Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences and Okayama University Hospital
kn-affil=

affil-num=8
en-affil=Department of Research Promotion and Management, National Cerebral and Cardiovascular Center
kn-affil=

affil-num=9
en-affil=Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences and Okayama University Hospital
kn-affil=
en-keyword=Sodium-glucose-cotransporter 2 inhibitor
kn-keyword=Sodium-glucose-cotransporter 2 inhibitor
en-keyword=Dapagliflozin
kn-keyword=Dapagliflozin
en-keyword=Myocardial ischemia/reperfusion
kn-keyword=Myocardial ischemia/reperfusion
en-keyword=Cardiac microdialysis
kn-keyword=Cardiac microdialysis
en-keyword=Myoglobin
kn-keyword=Myoglobin
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=5
article-no=
start-page=763
end-page=779
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240223
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=GRP78 Contributes to the Beneficial Effects of SGLT2 Inhibitor on Proximal Tubular Cells in DKD
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The beneficial effects of sodium–glucose cotransporter 2 (SGLT2) inhibitors on kidney function are well-known; however, their molecular mechanisms are not fully understood. We focused on 78-kDa glucose-regulated protein (GRP78) and its interaction with SGLT2 and integrin-β1 beyond the chaperone property of GRP78. In streptozotocin (STZ)-induced diabetic mouse kidneys, GRP78, SGLT2, and integrin-β1 increased in the plasma membrane fraction, while they were suppressed by canagliflozin. The altered subcellular localization of GRP78/integrin-β1 in STZ mice promoted epithelial mesenchymal transition (EMT) and fibrosis, which were mitigated by canagliflozin. High-glucose conditions reduced intracellular GRP78, increased its secretion, and caused EMT-like changes in cultured HK2 cells, which were again inhibited by canagliflozin. Urinary GRP78 increased in STZ mice, and in vitro experiments with recombinant GRP78 suggested that inflammation spread to surrounding tubular cells and that canagliflozin reversed this effect. Under normal glucose culture, canagliflozin maintained sarco/endoplasmic reticulum (ER) Ca2+-ATPase (SERCA) activity, promoted ER robustness, reduced ER stress response impairment, and protected proximal tubular cells. In conclusion, canagliflozin restored subcellular localization of GRP78, SGLT2, and integrin-β1 and inhibited EMT and fibrosis in DKD. In nondiabetic chronic kidney disease, canagliflozin promoted ER robustness by maintaining SERCA activity and preventing ER stress response failure, and it contributed to tubular protection.
en-copyright=
kn-copyright=

en-aut-name=NakatsukaAtsuko
en-aut-sei=Nakatsuka
en-aut-mei=Atsuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamaguchiSatoshi
en-aut-sei=Yamaguchi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Division of Kidney, Diabetes and Endocrine Diseases, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=45
cd-vols=
no-issue=11
article-no=
start-page=1596
end-page=1601
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221101
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Investigation of the Expression of Serine Protease in Vibrio vulnificus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vibrio vulnificus is a Gram-negative estuarine bacterium that causes infection in immuno-compromised patients, eels, and shrimp. V. vulnificus NCIMB2137, a metalloprotease-negative strain isolated from a diseased eel, produces a 45-kDa chymotrypsin-like alkaline serine protease known as VvsA. The gene encoding vvsA also includes another gene, vvsB with an unknown function; however, it is assumed to be an essential molecular chaperone for the maturation of VvsA. In the present study, we used an in vitro cell-free translation system to examine the maturation pathway of VvsA. We individually expressed the vvsA and vvsB genes and detected their mRNAs. However, the sample produced from vvsA did not exhibit protease activity. A sodium dodecyl sulfate (SDS) analysis detected the VvsB protein, but not the VvsA protein. A Western blotting analysis using a histidine (His)-tag at the amino terminus of proteins also showed no protein production by vvsA. These results suggested the translation, but not the transcription of vvsA. Factors derived from Escherichia coli were used in the in vitro cell-free translation system employed in the present study. The operon of the serine protease gene containing vvsA and vvsB was expressed in E. coli. Although serine proteases were produced, they were cleaved at different sites and no active mature forms were detected. These results indicate that the operon encoding vvsA and vvsB is a gene constructed to be specifically expressed in V. vulnificus.
en-copyright=
kn-copyright=

en-aut-name=KawaseTomoka
en-aut-sei=Kawase
en-aut-mei=Tomoka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=DebnathAnusuya
en-aut-sei=Debnath
en-aut-mei=Anusuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MizunoTamaki
en-aut-sei=Mizuno
en-aut-mei=Tamaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MiyakeYui
en-aut-sei=Miyake
en-aut-mei=Yui
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=Vibrio vulnificus serine protease
kn-keyword=Vibrio vulnificus serine protease
en-keyword=intermolecular chaperone
kn-keyword=intermolecular chaperone
en-keyword=cell-free translation system
kn-keyword=cell-free translation system
END

start-ver=1.4
cd-journal=joma
no-vol=17
cd-vols=
no-issue=5
article-no=
start-page=1215
end-page=1224
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230726
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Oxidative stress-related markers as prognostic factors for patients with primary sclerosing cholangitis in Japan
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background/purpose Primary sclerosing cholangitis (PSC) is a rare chronic liver disease. The mechanisms and prediction of PSC progression are unclear. Recent investigations have shown that general conditions, such as oxidative stress, affect the course of chronic diseases. We investigated the clinical course and oxidative stress-related condition of PSC to determine prognostic factors.<br>
Methods We recruited 58 patients with PSC (mean age; 37.4 years, mean observation period; 1382 days) who visited our department from 2003 to 2021. Clinical characteristics were investigated to define prognostic factors. Oxidative stress status was evaluated using two types of markers: an oxidative stress marker (serum reactive oxygen metabolite; dROM) and an antioxidant marker (serum OXY adsorbent test; OXY).<br>
Results The revised Mayo risk, Child–Pugh, model for end-stage liver disease-sodium (MELD-Na) scores or fibrosis-related FIB-4 index significantly predicted poor overall survival. High intestinal immunoglobulin A (IgA) levels predicted poor survival. Among patients with high and intermediate revised Mayo risk scores, those with physiologically high dROM levels showed better survival than those with lower dROM levels. In this population, dROM was negatively correlated with AST and IgA, which are both correlated with survival.<br>
Conclusions High and intermediate revised Mayo risk score group predicted a poor clinical course in PSC. Additionally, the Child–Pugh score, MELD-Na score, FIB-4 index, and serum IgA were significantly correlated with survival. In patients with high and intermediate revised Mayo risk scores, physiologically high oxidative stress status correlated with low IgA levels and a good prognosis.<br>
en-copyright=
kn-copyright=

en-aut-name=OyamaAtsushi
en-aut-sei=Oyama
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakakiAkinobu
en-aut-sei=Takaki
en-aut-mei=Akinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AdachiTakuya
en-aut-sei=Adachi
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WadaNozomu
en-aut-sei=Wada
en-aut-mei=Nozomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakeuchiYasuto
en-aut-sei=Takeuchi
en-aut-mei=Yasuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OnishiHideki
en-aut-sei=Onishi
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=ShirahaHidenori
en-aut-sei=Shiraha
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OtsukaMotoyuki
en-aut-sei=Otsuka
en-aut-mei=Motoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterology and Hepatology, Okayama University, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=Primary sclerosing cholangitis
kn-keyword=Primary sclerosing cholangitis
en-keyword=Oxidative stress marker
kn-keyword=Oxidative stress marker
en-keyword=Prognosis
kn-keyword=Prognosis
en-keyword=Serum reactive oxygen metabolite
kn-keyword=Serum reactive oxygen metabolite
en-keyword=Total serum antioxidant capacity
kn-keyword=Total serum antioxidant capacity
en-keyword=Revised Mayo risk score
kn-keyword=Revised Mayo risk score
en-keyword=Child–Pugh score
kn-keyword=Child–Pugh score
en-keyword=MELD score
kn-keyword=MELD score
en-keyword=FIB-4 index
kn-keyword=FIB-4 index
en-keyword=Serum dROM
kn-keyword=Serum dROM
en-keyword=Serum OXY-adsorbent test
kn-keyword=Serum OXY-adsorbent test
en-keyword=Immunoglobulin A
kn-keyword=Immunoglobulin A
END

start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=2
article-no=
start-page=182
end-page=194
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20231208
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Inhibition of Amino Acids Influx into Proximal Tubular Cells Improves Lysosome Function in Diabetes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background Inhibition of glucose influx into proximal tubular cells (PTCs) by sodium–glucose cotransporter 2 inhibitors revealed prominent therapeutic effects on diabetic kidney disease. Collectrin (CLTRN) serves as a chaperone for the trafficking of neutral amino acid (AA) transporters in the apical membranes of PTCs. We investigated the beneficial effects of reduced influx of AAs into PTCs in diabetes and obesity model of Cltrn−/y mice.<br>
Methods Cltrn+/y and Cltrn−/y mice at age 5 weeks were assigned to standard diet and streptozotocin and high-fat diet (STZ-HFD)–treated groups.<br>
Results At age 22–23 weeks, body weight and HbA1c levels significantly increased in STZ-HFD-Cltrn+/y compared with standard diet-Cltrn+/y; however, they were not altered in STZ-HFD-Cltrn−/y compared with STZ-HFD-Cltrn+/y. At age 20 weeks, urinary albumin creatinine ratio was significantly reduced in STZ-HFD-Cltrn−/y compared with STZ-HFD-Cltrn+/y. Under the treatments with STZ and HFD, the Cltrn gene deficiency caused significant increase in urinary concentration of AAs such as Gln, His, Gly, Thr, Tyr, Val, Trp, Phe, Ile, Leu, and Pro. In PTCs in STZ-HFD-Cltrn+/y, the enlarged lysosomes with diameter of 10 μm or more were associated with reduced autolysosomes, and the formation of giant lysosomes was prominently suppressed in STZ-HFD-Cltrn−/y. Phospho-mTOR and inactive form of phospho-transcription factor EB were reduced in STZ-HFD-Cltrn−/y compared with STZ-HFD-Cltrn+/y.<br>
Conclusions The reduction of AAs influx into PTCs inactivated mTOR, activated transcription factor EB, improved lysosome function, and ameliorated vacuolar formation of PTCs in STZ-HFD-Cltrn−/y mice.
en-copyright=
kn-copyright=

en-aut-name=KanoYuzuki
en-aut-sei=Kano
en-aut-mei=Yuzuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamaguchiSatoshi
en-aut-sei=Yamaguchi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MiseKoki
en-aut-sei=Mise
en-aut-mei=Koki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KawakitaChieko
en-aut-sei=Kawakita
en-aut-mei=Chieko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OnishiYasuhiro
en-aut-sei=Onishi
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KurookaNaoko
en-aut-sei=Kurooka
en-aut-mei=Naoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SugawaraRyosuke
en-aut-sei=Sugawara
en-aut-mei=Ryosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=AlbuayjanHaya Hamed Hassan
en-aut-sei=Albuayjan
en-aut-mei=Haya Hamed Hassan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NakatsukaAtsuko
en-aut-sei=Nakatsuka
en-aut-mei=Atsuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=EguchiJun
en-aut-sei=Eguchi
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=9
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=10
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=11
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=diabetes mellitus
kn-keyword=diabetes mellitus
en-keyword=diabetic nephropathy
kn-keyword=diabetic nephropathy
en-keyword=metabolism
kn-keyword=metabolism
en-keyword=obesity
kn-keyword=obesity
en-keyword=tubular epithelium
kn-keyword=tubular epithelium
END

start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=7
article-no=
start-page=1190
end-page=1202
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220421
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Reduction in the magnitude of serum potassium elevation in combination therapy with esaxerenone (CS‐3150) and sodium–glucose cotransporter 2 inhibitor in patients with diabetic kidney disease: Subanalysis of two phase III studies
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aims/Introduction: We evaluated the effect of co-administration of esaxerenone and a sodium–glucose cotransporter 2 (SGLT2) inhibitor on the magnitude of serum potassium elevation in Japanese patients with diabetic kidney disease.<br>
Materials and Methods: We carried out a prespecified subanalysis of data from two phase III studies: a multicenter, randomized, double-blind, placebo-controlled trial in patients with type 2 diabetes and microalbuminuria (J308); and a multicenter, single-arm, open-label trial in patients with type 2 diabetes and macroalbuminuria (J309). Changes in serum potassium levels during the studies and other measures were evaluated according to SGLT2 inhibitor use.<br>
Results: In both studies, time-course changes in serum potassium levels, and incidence rates of serum potassium elevation were lower in patients with co-administration of SGLT2 inhibitor in both the placebo and esaxerenone groups than those without the inhibitor. In contrast, time-course changes and mean percentage changes from baseline in urinary albumin-to-creatinine ratio, the proportion of patients with albuminuria remission and time-course changes in blood pressure did not change with or without SGLT2 inhibitor, whereas the albumin-to-creatinine ratio and blood pressure were reduced with esaxerenone. The blood glucose-lowering effect of SGLT2 inhibitor was not affected by esaxerenone.<br>
Conclusions: In Japanese patients with type 2 diabetes and albuminuria treated with esaxerenone, concomitant use of SGLT2 inhibitor reduced the magnitude of serum potassium elevation without any change of its antihypertensive and albuminuria-suppressing effects. Co-administration of esaxerenone and SGLT2 inhibitor might be a beneficial treatment option for patients with diabetic kidney disease.
en-copyright=
kn-copyright=

en-aut-name=ShikataKenichi
en-aut-sei=Shikata
en-aut-mei=Kenichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ItoSadayoshi
en-aut-sei=Ito
en-aut-mei=Sadayoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KashiharaNaoki
en-aut-sei=Kashihara
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NangakuMasaomi
en-aut-sei=Nangaku
en-aut-mei=Masaomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WadaTakashi
en-aut-sei=Wada
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OkudaYasuyuki
en-aut-sei=Okuda
en-aut-mei=Yasuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SawanoboriTomoko
en-aut-sei=Sawanobori
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=SugimotoKotaro
en-aut-sei=Sugimoto
en-aut-mei=Kotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Division of Nephrology, Endocrinology and Vascular Medicine, Department of Medicine, Tohoku University School of Medicine
kn-affil=

affil-num=3
en-affil=Department of Nephrology and Hypertension, Kawasaki Medical School
kn-affil=

affil-num=4
en-affil=Division of Nephrology and Endocrinology, Graduate School of Medicine, The University of Tokyo
kn-affil=

affil-num=5
en-affil=Department of Nephrology and Laboratory Medicine, Kanazawa University
kn-affil=

affil-num=6
en-affil=Department of Nephrology and Laboratory Medicine, Kanazawa University
kn-affil=

affil-num=7
en-affil=Clinical Development Department, Daiichi Sankyo Co., Ltd.
kn-affil=

affil-num=8
en-affil=Primary Medical Science Department, Daiichi Sankyo Co., Ltd.
kn-affil=
en-keyword=Esaxerenone
kn-keyword=Esaxerenone
en-keyword=Potassium
kn-keyword=Potassium
en-keyword=Sodium-glucose transporter 2 inhibitor
kn-keyword=Sodium-glucose transporter 2 inhibitor
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=2
article-no=
start-page=154
end-page=164
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Identification of a Functionally Efficient and Thermally Stable Outward Sodium-Pumping Rhodopsin (BeNaR) from a Thermophilic Bacterium
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Rhodopsins are transmembrane proteins with retinal chromophores that are involved in photo-energy conversion and photo-signal transduction in diverse organisms. In this study, we newly identified and characterized a rhodopsin from a thermophilic bacterium, Bellilinea sp. Recombinant Escherichia coli cells expressing the rhodopsin showed light-induced alkalization of the medium only in the presence of sodium ions (Na+), and the alkalization signal was enhanced by addition of a protonophore, indicating an outward Na+ pump function across the cellular membrane. Thus, we named the protein Bellilinea Na+-pumping rhodopsin, BeNaR. Of note, its Na+-pumping activity is significantly greater than that of the known Na+-pumping rhodopsin, KR2. We further characterized its photochemical properties as follows: (i) Visible spectroscopy and HPLC revealed that BeNaR has an absorption maximum at 524 nm with predominantly (>96%) the all-trans retinal conformer. (ii) Time-dependent thermal denaturation experiments revealed that BeNaR showed high thermal stability. (iii) The time-resolved flash-photolysis in the nanosecond to millisecond time domains revealed the presence of four kinetically distinctive photointermediates, K, L, M and O. (iv) Mutational analysis revealed that Asp101, which acts as a counterion, and Asp230 around the retinal were essential for the Na+-pumping activity. From the results, we propose a model for the outward Na+-pumping mechanism of BeNaR. The efficient Na+-pumping activity of BeNaR and its high stability make it a useful model both for ion transporters and optogenetics tools.
en-copyright=
kn-copyright=

en-aut-name=KuriharaMarie
en-aut-sei=Kurihara
en-aut-mei=Marie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ThielVera
en-aut-sei=Thiel
en-aut-mei=Vera
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakahashiHirona
en-aut-sei=Takahashi
en-aut-mei=Hirona
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KojimaKeiichi
en-aut-sei=Kojima
en-aut-mei=Keiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WardDavid M.
en-aut-sei=Ward
en-aut-mei=David M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=BryantDonald A.
en-aut-sei=Bryant
en-aut-mei=Donald A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SakaiMakoto
en-aut-sei=Sakai
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YoshizawaSusumu
en-aut-sei=Yoshizawa
en-aut-mei=Susumu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SudoYuki
en-aut-sei=Sudo
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Biological Sciences, Tokyo Metropolitan University
kn-affil=

affil-num=3
en-affil=Department of Chemistry, Graduate School of Science, Okayama University of Science
kn-affil=

affil-num=4
en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Land Resources and Environmental Sciences, Montana State University
kn-affil=

affil-num=6
en-affil=Department of Biochemistry and Molecular Biology, The Pennsylvania State University
kn-affil=

affil-num=7
en-affil=Department of Chemistry, Graduate School of Science, Okayama University of Science
kn-affil=

affil-num=8
en-affil=Atmosphere and Ocean Research Institute, The University of Tokyo
kn-affil=

affil-num=9
en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=rhodopsin
kn-keyword=rhodopsin
en-keyword=ion transport
kn-keyword=ion transport
en-keyword=retinal
kn-keyword=retinal
en-keyword=isomerization
kn-keyword=isomerization
en-keyword=optogenetics
kn-keyword=optogenetics
END

start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=
article-no=
start-page=1261330
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230907
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Introduction: Transplantation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is a promising treatment for heart failure. Information on long-term cell engraftment after transplantation is clinically important. However, clinically applicable evaluation methods have not yet been established.<br>
Methods: In this study, to noninvasively assess transplanted cell engraftment, human SLC5A5, which encodes a sodium/iodide symporter (NIS) that transports radioactive tracers such as 125I, 18F-tetrafluoroborate (TFB), and 99mTc-pertechnetate (99mTcO4−), was transduced into human induced pluripotent stem cells (iPSCs), and nuclear medicine imaging was used to track engrafted human iPSC-CMs.<br>
Results: To evaluate the pluripotency of NIS-expressing human iPSCs, they were subcutaneously transplanted into immunodeficient rats. Teratomas were detected by 99mTcO4− single photon emission computed tomography (SPECT/CT) imaging. NIS expression and the uptake ability of 125I were maintained in purified human iPSC-CMs. NIS-expressing human iPSC-CMs transplanted into immunodeficient rats could be detected over time using 99mTcO4− SPECT/CT imaging. Unexpectedly, NIS expression affected cell proliferation of human iPSCs and iPSC-derived cells.<br>
Discussion: Such functionally designed iPSC-CMs have potential clinical applications as a noninvasive method of grafted cell evaluation, but further studies are needed to determine the effects of NIS transduction on cellular characteristics and functions.
en-copyright=
kn-copyright=

en-aut-name=SaitoYukihiro
en-aut-sei=Saito
en-aut-mei=Yukihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NoseNaoko
en-aut-sei=Nose
en-aut-mei=Naoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=IidaToshihiro
en-aut-sei=Iida
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AkazawaKaoru
en-aut-sei=Akazawa
en-aut-mei=Kaoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KannoTakayuki
en-aut-sei=Kanno
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=FujimotoYuki
en-aut-sei=Fujimoto
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SasakiTakanori
en-aut-sei=Sasaki
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=AkehiMasaru
en-aut-sei=Akehi
en-aut-mei=Masaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=HiguchiTakahiro
en-aut-sei=Higuchi
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=AkagiSatoshi
en-aut-sei=Akagi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=YoshidaMasashi
en-aut-sei=Yoshida
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Okayama Medical Innovation Center, Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Okayama Medical Innovation Center, Faculty of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=10
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=11
en-affil=Department of Chronic Kidney Disease and Cardiovascular Disease, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=12
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=13
en-affil=Department of General Internal Medicine 3, Kawasaki Medical School
kn-affil=

affil-num=14
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=sodium/iodide symporter
kn-keyword=sodium/iodide symporter
en-keyword=human induced pluripotent stem cell-derived cardiomyocytes
kn-keyword=human induced pluripotent stem cell-derived cardiomyocytes
en-keyword=single photon emission computed tomography
kn-keyword=single photon emission computed tomography
en-keyword=cell-based therapy
kn-keyword=cell-based therapy
en-keyword=in vivo imaging
kn-keyword=in vivo imaging
END

start-ver=1.4
cd-journal=joma
no-vol=24
cd-vols=
no-issue=8
article-no=
start-page=1429
end-page=1438
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220518
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Rationale, design and baseline characteristics of the effect of canagliflozin in patients with type 2 diabetes and microalbuminuria in the Japanese population: The CANPIONE study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aim: To evaluate the effect of canagliflozin, a sodium-glucose co-transporter-2 (SGLT2) inhibitor, on albuminuria and the decline of estimated glomerular filtration rate (eGFR) in participants with type 2 diabetes and microalbuminuria.<br>
Methods: The CANPIONE study is a multicentre, randomized, parallel-group and open-labelled study consisting of a unique 24-week preintervention period, during which the rate of eGFR decline before intervention is estimated, followed by a 52-week intervention and a 4-week washout period. Participants with a geometric mean urinary albumin-to-creatinine ratio (UACR) of 50 and higher and less than 300 mg/g in two consecutive first-morning voids at two different time points, and an eGFR of 45 ml/min/1.73m2 or higher, are randomly assigned to receive canagliflozin 100 mg daily or to continue guideline-recommended treatment, except for SGLT2 inhibitors. The first primary outcome is the change in UACR, and the second primary outcome is the change in eGFR slope.<br>
Results: A total of 258 participants were screened and 98 were randomized at 21 sites in Japan from August 2018 to May 2021. The mean baseline age was 61.4 years and 25.8% were female. The mean HbA1c was 7.9%, mean eGFR was 74.1 ml/min/1.73m2 and median UACR was 104.2 mg/g.<br>
Conclusions: The CANPIONE study will determine whether the SGLT2 inhibitor canagliflozin can reduce albuminuria and slow eGFR decline in participants with type 2 diabetes and microalbuminuria.
en-copyright=
kn-copyright=

en-aut-name=MiyamotoSatoshi
en-aut-sei=Miyamoto
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HeerspinkHiddo J. L.
en-aut-sei=Heerspink
en-aut-mei=Hiddo J. L.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=de ZeeuwDick
en-aut-sei=de Zeeuw
en-aut-mei=Dick
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ToyodaMasao
en-aut-sei=Toyoda
en-aut-mei=Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SuzukiDaisuke
en-aut-sei=Suzuki
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HatanakaTakashi
en-aut-sei=Hatanaka
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NakamuraTohru
en-aut-sei=Nakamura
en-aut-mei=Tohru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KameiShinji
en-aut-sei=Kamei
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=MuraoSatoshi
en-aut-sei=Murao
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HidaKazuyuki
en-aut-sei=Hida
en-aut-mei=Kazuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=AndoShinichiro
en-aut-sei=Ando
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=AkaiHiroaki
en-aut-sei=Akai
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=TakahashiYasushi
en-aut-sei=Takahashi
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=KoyaDaisuke
en-aut-sei=Koya
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=KitadaMunehiro
en-aut-sei=Kitada
en-aut-mei=Munehiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=SuganoHisashi
en-aut-sei=Sugano
en-aut-mei=Hisashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=NunoueTomokazu
en-aut-sei=Nunoue
en-aut-mei=Tomokazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=NakamuraAkihiko
en-aut-sei=Nakamura
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=SasakiMotofumi
en-aut-sei=Sasaki
en-aut-mei=Motofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=NakatouTatsuaki
en-aut-sei=Nakatou
en-aut-mei=Tatsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=FujimotoKei
en-aut-sei=Fujimoto
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=KawanamiDaiji
en-aut-sei=Kawanami
en-aut-mei=Daiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

en-aut-name=WadaTakashi
en-aut-sei=Wada
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=

en-aut-name=MiyatakeNobuyuki
en-aut-sei=Miyatake
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=24
ORCID=

en-aut-name=YoshidaMichihiro
en-aut-sei=Yoshida
en-aut-mei=Michihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=25
ORCID=

en-aut-name=ShikataKenichi
en-aut-sei=Shikata
en-aut-mei=Kenichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=26
ORCID=

en-aut-name=the CANPIONE study Investigators
en-aut-sei=the CANPIONE study Investigators
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=27
ORCID=

affil-num=1
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen
kn-affil=

affil-num=3
en-affil=Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen
kn-affil=

affil-num=4
en-affil=Division of Nephrology, Endocrinology and Metabolism, Department of Internal Medicine, Tokai University School of Medicine
kn-affil=

affil-num=5
en-affil=Suzuki Diadetes Clinic
kn-affil=

affil-num=6
en-affil=Department of Diabetes and Endocrinology, National Hospital Organization Fukuyama Medical Center
kn-affil=

affil-num=7
en-affil=Diabetes Internal Medicine, Sumitomo Besshi Hospital
kn-affil=

affil-num=8
en-affil=Department of Diabetic Medicine, Kurashiki Central Hospital
kn-affil=

affil-num=9
en-affil=Department of Diabetes and Endocrinology, Takamatsu Hospital
kn-affil=

affil-num=10
en-affil=Department of Diabetology and Metabolism, National Hospital Organization Okayama Medical Center
kn-affil=

affil-num=11
en-affil=Department of Internal Medicine Diabetic Center, Okayama City Hospital
kn-affil=

affil-num=12
en-affil=Division of Diabetes and Metabolism, Faculty of Medicine, Tohoku Medical and Pharmaceutical University
kn-affil=

affil-num=13
en-affil=Department of Diabetes, Ochiai General Hospital
kn-affil=

affil-num=14
en-affil=Department of Diabetology and Endocrinology, Kanazawa Medical University
kn-affil=

affil-num=15
en-affil=Department of Diabetology and Endocrinology, Kanazawa Medical University
kn-affil=

affil-num=16
en-affil=Department of Diabetes and Endocrinology, Kochi Health Sciences Center
kn-affil=

affil-num=17
en-affil=Nunoue Clinic
kn-affil=

affil-num=18
en-affil=Internal Medicine, Osafune Clinic, Setouchi
kn-affil=

affil-num=19
en-affil=Department of Diabetes and Endocrinology, Matsue City Hospital
kn-affil=

affil-num=20
en-affil=Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=21
en-affil=Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, The Jikei University Kashiwa Hospital
kn-affil=

affil-num=22
en-affil=Department of Endocrinology and Diabetes Mellitus, Fukuoka University School of Medicine
kn-affil=

affil-num=23
en-affil=Department of Nephrology and Laboratory Medicine, Graduate School of Medical Sciences, Kanazawa University
kn-affil=

affil-num=24
en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=25
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=26
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=

affil-num=27
en-affil=
kn-affil=
en-keyword=canagliflozin
kn-keyword=canagliflozin
en-keyword=CANPIONE study
kn-keyword=CANPIONE study
en-keyword=diabetic kidney disease
kn-keyword=diabetic kidney disease
en-keyword=eGFR slope
kn-keyword=eGFR slope
en-keyword=SGLT2 inhibitor
kn-keyword=SGLT2 inhibitor
en-keyword=urinary albumin-to-creatinine ratio
kn-keyword=urinary albumin-to-creatinine ratio
END

start-ver=1.4
cd-journal=joma
no-vol=1706
cd-vols=
no-issue=
article-no=
start-page=464247
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230913
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Separation and fractionation of glutamic acid and histidine via origami isoelectric focusing
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We demonstrated the fractionation of two amino acids, glutamic acid and histidine, separated via isoelectric focusing (IEF) on filter paper folded and stacked in an origami fashion. Channels for electrophoresis were fabricated as circular zones acquired via wax printing onto the filter paper. An ampholyte solution with amphiphilic samples was deposited on all the circle zones, which was followed by folding to form the electrophoresis channels. IEF was achieved by applying an electrical potential between the anodic and cathodic chambers filled with phosphoric acid and sodium hydroxide solutions, respectively. A pH gradient was formed using either a wide-range ampholyte with a pH of 3 to 10 or a narrow-range version with a pH of 5 to 8, which was confirmed by adding pH indicators to each layer. The origami IEF was used to separate the amino acids, glutamic acid and histidine, by mixing with the ampholytes, which were deposited on the layers. The components in each layer were extracted with water and measured by high-performance liquid chromatography using pre-column derivatization with dansyl chloride. The results indicated that the focus for glutamic acid and that for histidine were at different layers, according to their isoelectric points. The origami isoelectric focusing achieved the fractionation of amino acids in less than 3 min using voltage as low as 30 V.
en-copyright=
kn-copyright=

en-aut-name=DanchanaKaewta
en-aut-sei=Danchana
en-aut-mei=Kaewta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamashitaNayu
en-aut-sei=Yamashita
en-aut-mei=Nayu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UmedaMika I.
en-aut-sei=Umeda
en-aut-mei=Mika I.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Chemistry, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Chemistry, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Chemistry, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Chemistry, Okayama University
kn-affil=
en-keyword=Paper-based analytical device
kn-keyword=Paper-based analytical device
en-keyword=Isoelectric focusing
kn-keyword=Isoelectric focusing
en-keyword=Origami electrophoresis
kn-keyword=Origami electrophoresis
en-keyword=Amino acids
kn-keyword=Amino acids
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=4
article-no=
start-page=359
end-page=364
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=202308
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Changes in TRPV1 Receptor, CGRP, and BDNF Expression in Rat Dorsal Root Ganglion with Resiniferatoxin-Induced Neuropathic Pain: Modulation by Pulsed Radiofrequency Applied to the Sciatic Nerve
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Pulsed radiofrequency (PRF) is a safe method of treating neuropathic pain by generating intermittent electric fields at the needle tip. Resiniferatoxin (RTX) is an ultrapotent agonist of transient receptor potential vanilloid subtype-1 (TRPV1) receptors. We investigated the mechanism of PRF using a rat model of RTX-induced neuropathic pain. After administering RTX intraperitoneally, PRF was applied to the right sciatic nerve. We observed the changes in TRPV1, calcitonin gene-related peptide (CGRP), and brain-derived neurotrophic factor (BDNF) in the dorsal root ganglia by western blotting. Expressions of TRPV1 and CGRP were significantly lower in the contralateral (RTX-treated, PRF-untreated) tissue than in control rats (p<0.0001 and p<0.0001, respectively) and the ipsilateral tissues (p<0.0001 and p<0.0001, respectively). BDNF levels were significantly higher in the contralateral tissues than in the control rats (p<0.0001) and the ipsilateral tissues (p<0.0001). These results suggest that, while TRPV1 and CGRP are decreased by RTX-induced neuronal damage, increased BDNF levels result in pain development. PRF may promote recovery from neuronal damage with concomitant restoration of TRPV1 and CGRP, and exert its analgesic effect by reversing BDNF increase. Further research is required to understand the role of TRPV1 and CGRP restoration in improving mechanical allodynia.
en-copyright=
kn-copyright=

en-aut-name=KoshidaTomohiro
en-aut-sei=Koshida
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MarutaToyoaki
en-aut-sei=Maruta
en-aut-mei=Toyoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanakaNobuhiko
en-aut-sei=Tanaka
en-aut-mei=Nobuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HidakaKotaro
en-aut-sei=Hidaka
en-aut-mei=Kotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KurogiMio
en-aut-sei=Kurogi
en-aut-mei=Mio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NemotoTakayuki
en-aut-sei=Nemoto
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YanagitaToshihiko
en-aut-sei=Yanagita
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TakeyaRyu
en-aut-sei=Takeya
en-aut-mei=Ryu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TsuneyoshiIsao
en-aut-sei=Tsuneyoshi
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki
kn-affil=

affil-num=2
en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki
kn-affil=

affil-num=3
en-affil=Tanaka homecare clinic
kn-affil=

affil-num=4
en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki
kn-affil=

affil-num=5
en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki
kn-affil=

affil-num=6
en-affil=Department of Pharmacology, Faculty of Medicine, Fukuoka University
kn-affil=

affil-num=7
en-affil=Department of Clinical Pharmacology, School of Nursing, Faculty of Medicine, University of Miyazaki
kn-affil=

affil-num=8
en-affil=Department of Pharmacology, Faculty of Medicine, University of Miyazaki
kn-affil=

affil-num=9
en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki
kn-affil=
en-keyword=pulsed radiofrequency
kn-keyword=pulsed radiofrequency
en-keyword=resiniferatoxin
kn-keyword=resiniferatoxin
en-keyword=transient receptor potential vanilloid subtype-1 (TRPV1)
kn-keyword=transient receptor potential vanilloid subtype-1 (TRPV1)
en-keyword=calcitonin gene-related peptide (CGRP)
kn-keyword=calcitonin gene-related peptide (CGRP)
en-keyword=brain-derived neurotrophic factor (BDNF)
kn-keyword=brain-derived neurotrophic factor (BDNF)
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=10
article-no=
start-page=3848
end-page=3856
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230723
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Combination therapy with hydrogen peroxide and irradiation promotes an abscopal effect in mouse models
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hydrogen peroxide (H2O2) induces oxidative stress and cytotoxicity, and can be used for treating cancers in combination with radiotherapy. A product comprising H2O2 and sodium hyaluronate has been developed as a radiosensitizer. However, the effects of H2O2 on antitumor immunity remain unclear. To investigate the effects of H2O2, especially the abscopal effect when combined with radiotherapy (RT), we implanted murine tumor cells simultaneously in two locations in mouse models: the hind limb and back. H2O2 mixed with sodium hyaluronate was injected intratumorally, followed by irradiation only at the hind limb lesion. No treatment was administered to the back lesion. The H2O2/RT combination significantly reduced tumor growth at the noninjected/nonirradiated site in the back lesion, whereas H2O2 or RT individually did not reduce tumor growth. Flow cytometric analyses of the tumor-draining lymph nodes in the injected/irradiated areas showed that the number of dendritic cells increased significantly with maturation in the H2O2/RT combination group. In addition, analyses of tumor-infiltrating lymphocytes showed that the number of CD8+ (cluster of differentiation 8) T cells and the frequency of IFN-γ+ (interferon gamma) CD8+ T cells were higher in the noninjected/nonirradiated tumors in the H2O2/RT group compared to those in the other groups. PD-1 (programmed death receptor 1) blockade further increased the antitumor effect against noninjected/nonirradiated tumors in the H2O2/RT group. Intratumoral injection of H2O2 combined with RT therefore induces an abscopal effect by activating antitumor immunity, which can be further enhanced by PD-1 blockade. These findings promote the development of H2O2/RT therapy combined with cancer immunotherapies, even for advanced cancers.
en-copyright=
kn-copyright=

en-aut-name=KemmotsuNaoya
en-aut-sei=Kemmotsu
en-aut-mei=Naoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ZhuLi
en-aut-sei=Zhu
en-aut-mei=Li
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NagasakiJoji
en-aut-sei=Nagasaki
en-aut-mei=Joji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OtaniYoshihiro
en-aut-sei=Otani
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=UedaYouki
en-aut-sei=Ueda
en-aut-mei=Youki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=DansakoHiromichi
en-aut-sei=Dansako
en-aut-mei=Hiromichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=FangYue
en-aut-sei=Fang
en-aut-mei=Yue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=DateIsao
en-aut-sei=Date
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TogashiYosuke
en-aut-sei=Togashi
en-aut-mei=Yosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Tumor Microenvironment, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Tumor Microenvironment, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Tumor Microenvironment, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Neurological Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Tumor Microenvironment, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Tumor Microenvironment, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Microbial and Biochemical Pharmacy, School of Pharmacy, China Medical University
kn-affil=

affil-num=8
en-affil=Department of Neurological Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=9
en-affil=Department of Tumor Microenvironment, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=abscopal effect
kn-keyword=abscopal effect
en-keyword=dendritic cell
kn-keyword=dendritic cell
en-keyword=hydrogen peroxide
kn-keyword=hydrogen peroxide
en-keyword=radiosensitizer
kn-keyword=radiosensitizer
en-keyword=radiotherapy
kn-keyword=radiotherapy
en-keyword=tumor-draining lymph node
kn-keyword=tumor-draining lymph node
END

start-ver=1.4
cd-journal=joma
no-vol=15
cd-vols=
no-issue=13
article-no=
start-page=2941
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230628
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Adrenomedullin Enhances Mouse Gustatory Nerve Responses to Sugars via T1R-Independent Sweet Taste Pathway
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=On the tongue, the T1R-independent pathway (comprising glucose transporters, including sodium-glucose cotransporter (SGLT1) and the K-ATP channel) detects only sugars, whereas the T1R-dependent (T1R2/T1R3) pathway can broadly sense various sweeteners. Cephalic-phase insulin release, a rapid release of insulin induced by sensory signals in the head after food-related stimuli, reportedly depends on the T1R-independent pathway, and the competitive sweet taste modulators leptin and endocannabinoids may function on these two different sweet taste pathways independently, suggesting independent roles of two oral sugar-detecting pathways in food intake. Here, we examined the effect of adrenomedullin (ADM), a multifunctional regulatory peptide, on sugar sensing in mice since it affects the expression of SGLT1 in rat enterocytes. We found that ADM receptor components were expressed in T1R3-positive taste cells. Analyses of chorda tympani (CT) nerve responses revealed that ADM enhanced responses to sugars but not to artificial sweeteners and other tastants. Moreover, ADM increased the apical uptake of a fluorescent D-glucose derivative into taste cells and SGLT1 mRNA expression in taste buds. These results suggest that the T1R-independent sweet taste pathway in mouse taste cells is a peripheral target of ADM, and the specific enhancement of gustatory nerve responses to sugars by ADM may contribute to caloric sensing and food intake.
en-copyright=
kn-copyright=

en-aut-name=IwataShusuke
en-aut-sei=Iwata
en-aut-mei=Shusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YoshidaRyusuke
en-aut-sei=Yoshida
en-aut-mei=Ryusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakaiShingo
en-aut-sei=Takai
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SanematsuKeisuke
en-aut-sei=Sanematsu
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=ShigemuraNoriatsu
en-aut-sei=Shigemura
en-aut-mei=Noriatsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NinomiyaYuzo
en-aut-sei=Ninomiya
en-aut-mei=Yuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
kn-affil=

affil-num=2
en-affil=Department of Oral Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
kn-affil=

affil-num=4
en-affil=Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
kn-affil=

affil-num=5
en-affil=Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
kn-affil=

affil-num=6
en-affil=Department of Oral Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=taste
kn-keyword=taste
en-keyword=sweet taste
kn-keyword=sweet taste
en-keyword=taste receptor family 1 members 2 and 3
kn-keyword=taste receptor family 1 members 2 and 3
en-keyword=sodium-glucose cotransporter 1
kn-keyword=sodium-glucose cotransporter 1
en-keyword=adrenomedullin
kn-keyword=adrenomedullin
en-keyword=caloric sensing
kn-keyword=caloric sensing
END

start-ver=1.4
cd-journal=joma
no-vol=15
cd-vols=
no-issue=1
article-no=
start-page=84
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221227
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Formation of a Stable Co-Amorphous System for a Brick Dust Molecule by Utilizing Sodium Taurocholate with High Glass Transition Temperature
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Brick dust molecules are usually poorly soluble in water and lipoidal components, making it difficult to formulate them in dosage forms that provide efficient pharmacological effects. A co-amorphous system is an effective strategy to resolve these issues. However, their glass transition temperatures (Tg) are relatively lower than those of polymeric amorphous solid dispersions, suggesting the instability of the co-amorphous system. This study aimed to formulate a stable co-amorphous system for brick dust molecules by utilizing sodium taurocholate (NaTC) with a higher Tg. A novel neuropeptide Y-5 receptor antagonist (AntiY(5)R) and NaTC with Tg of 155 degrees C were used as the brick dust model and coformer, respectively. Ball milling formed a co-amorphous system for AntiY(5)R and NaTC (AntiY(5)R-NaTC) at various molar ratios. Deviation from the theoretical Tg value and peak shifts in Fourier-transform infrared spectroscopy indicated intermolecular interactions between AntiY(5)R and NaTC. AntiY(5)R-NaTC at equal molar ratios resulting in an 8.5-fold increase in AntiY(5)R solubility over its crystalline form. The co-amorphous system remained amorphous for 1 month at 25 degrees C and 40 degrees C. These results suggest that the co-amorphous system formed by utilizing NaTC as a coformer could stably maintain the amorphous state and enhance the solubility of brick dust molecules.
en-copyright=
kn-copyright=

en-aut-name=AikawaShohei
en-aut-sei=Aikawa
en-aut-mei=Shohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TanakaHironori
en-aut-sei=Tanaka
en-aut-mei=Hironori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UedaHiroshi
en-aut-sei=Ueda
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MaruyamaMasato
en-aut-sei=Maruyama
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HigakiKazutaka
en-aut-sei=Higaki
en-aut-mei=Kazutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Formulation Research Department, Formulation R&D Laboratory, Shionogi & Co., Ltd.
kn-affil=

affil-num=3
en-affil=Bioanalytical, Analysis and Evaluation Laboratory, Shionogi & Co., Ltd.
kn-affil=

affil-num=4
en-affil=Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=amorphous
kn-keyword=amorphous
en-keyword=co-amorphous
kn-keyword=co-amorphous
en-keyword=crystallization
kn-keyword=crystallization
en-keyword=sodium taurocholate
kn-keyword=sodium taurocholate
en-keyword=glass transition temperature
kn-keyword=glass transition temperature
en-keyword=intermolecular interaction
kn-keyword=intermolecular interaction
en-keyword=dissolution testing
kn-keyword=dissolution testing
END

start-ver=1.4
cd-journal=joma
no-vol=2022
cd-vols=
no-issue=
article-no=
start-page=4635171
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220621
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=In Vivo Functional Assessment of Sodium-Glucose Cotransporters (SGLTs) Using [F-18]Me4FDG PET in Rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background. Mediating glucose absorption in the small intestine and renal clearance, sodium glucose cotransporters (SGLTs) have emerged as an attractive therapeutic target in diabetic patients. A substantial fraction of patients, however, only achieve inadequate glycemic control. Thus, we aimed to assess the potential of the SGLT-targeting PET radiotracer alpha-methyl-4-deoxy-4-[F-18]fluoro-D-glucopyranoside ([F-18]Me4FDG) as a noninvasive intestinal and renal biomarker of SGLT-mediated glucose transport. Methods. We investigated healthy rats using a dedicated small animal PET system. Dynamic imaging was conducted after administration of the reference radiotracer 2-deoxy-2-[F-18]fluoro-D-glucose ([F-18]FDG), or the SGLT-targeting agent, [F-18]Me4FDG either directly into the digestive tract (for assessing intestinal absorption) or via the tail vein (for evaluating kidney excretion). To confirm the specificity of [F-18]Me4FDG and responsiveness to treatment, a subset of animals was also pretreated with the SGLT inhibitor phlorizin. In this regard, an intraintestinal route of administration was used to assess tracer absorption in the digestive tract, while for renal assessment, phlorizin was injected intravenously (IV). Results. Serving as reference, intestinal administration of [F-18]FDG led to slow absorption with retention of 89.2 +/- 3.5% of administered radioactivity at 15 min. [F-18]Me4FDG, however, was rapidly absorbed into the blood and cleared from the intestine within 15 min, leading to markedly lower tracer retention of 18.5 +/- 1.2% (P < 0.0001). Intraintestinal phlorizin led to marked increase of [F-18]Me4FDG uptake (15 min, 99.9 +/- 4.7%; P < 0.0001 vs. untreated controls), supporting the notion that this PET agent can measure adequate SGLT inhibition in the digestive tract. In the kidneys, radiotracer was also sensitive to SGLT inhibition. After IV injection, [F-18]Me4FDG reabsorption in the renal cortex was significantly suppressed by phlorizin when compared to untreated animals (%ID/g at 60 min, 0.42 +/- 0.10 vs. untreated controls, 1.20 +/- 0.03; P < 0.0001). Conclusion. As a noninvasive read-out of the concurrent SGLT expression in both the digestive tract and the renal cortex, [F-18]Me4FDG PET may serve as a surrogate marker for treatment response to SGLT inhibition. As such, [F-18]Me4FDG may enable improvement in glycemic control in diabetes by PET-based monitoring strategies.
en-copyright=
kn-copyright=

en-aut-name=MatsusakaYohji
en-aut-sei=Matsusaka
en-aut-mei=Yohji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ChenXinyu
en-aut-sei=Chen
en-aut-mei=Xinyu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Arias-LozaPaula
en-aut-sei=Arias-Loza
en-aut-mei=Paula
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WernerRudolf A.
en-aut-sei=Werner
en-aut-mei=Rudolf A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NoseNaoko
en-aut-sei=Nose
en-aut-mei=Naoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SasakiTakanori
en-aut-sei=Sasaki
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=RoweSteven P.
en-aut-sei=Rowe
en-aut-mei=Steven P.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=PomperMartin G.
en-aut-sei=Pomper
en-aut-mei=Martin G.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=LapaConstantin
en-aut-sei=Lapa
en-aut-mei=Constantin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HiguchiTakahiro
en-aut-sei=Higuchi
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=Department of Nuclear Medicine and Comprehensive Heart Failure Center, University Hospital of Würzburg
kn-affil=

affil-num=2
en-affil=Department of Nuclear Medicine and Comprehensive Heart Failure Center, University Hospital of Würzburg
kn-affil=

affil-num=3
en-affil=Department of Nuclear Medicine and Comprehensive Heart Failure Center, University Hospital of Würzburg
kn-affil=

affil-num=4
en-affil=Department of Nuclear Medicine and Comprehensive Heart Failure Center, University Hospital of Würzburg
kn-affil=

affil-num=5
en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Division of Nuclear Medicine and Molecular Imaging, The Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins School of Medicine
kn-affil=

affil-num=8
en-affil=Division of Nuclear Medicine and Molecular Imaging, The Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins School of Medicine
kn-affil=

affil-num=9
en-affil= Nuclear Medicine, Faculty of Medicine, University of Augsburg
kn-affil=

affil-num=10
en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=6
article-no=
start-page=715
end-page=721
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=202212
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Graphene Oxide-based Endodontic Sealer: An in Vitro Study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The failure of endodontic treatment is directly associated with microbial infection in the root canal or periapical areas. An endodontic sealer that is both bactericidal and biocompatible is essential for the success of root canal treatments. This is one of the vital issues yet to be solved in clinical dental practice. This in vitro study assessed the effectiveness of graphene oxide (GO) composites GO-CaF2 and GO-Ag-CaF2 as endodontic sealer materials. Dentin slices were coated with either the GO-based composites or commonly used root canal sealers (non-eugenol zinc oxide sealer). The coated slices were treated in 0.9% NaCl, phosphate-buffered saline (PBS), and simulated body fluid (SBF) at 37˚C for 24 hours to compare their sealing effect on the dentin surface. In addition, the radiopacity of these composites was examined to assess whether they complied with the requirements of a sealer for good radiographic visualization. Scanning electron microscopy showed the significant sealing capability of the composites as coating materials. Radiographic images confirmed their radiopacity. Mineral deposition indicated their bioactivity, especially of GO-Ag-CaF2, and thus it is potential for regenerative application. They were both previously shown to be bactericidal to oral microbes and cytocompatible with host cells. With such a unique assemblage of critical properties, these GO-based composites show promise as endodontic sealers for protection against reinfection in root canal treatment and enhanced success in endodontic treatment overall.
en-copyright=
kn-copyright=

en-aut-name=Mohammed Zahedul Islam Nizami
en-aut-sei=Mohammed Zahedul Islam Nizami
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=GorduysusMelahat
en-aut-sei=Gorduysus
en-aut-mei=Melahat
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Shinoda-ItoYuki
en-aut-sei=Shinoda-Ito
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YamamotoTadashi
en-aut-sei=Yamamoto
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NishinaYuta
en-aut-sei=Nishina
en-aut-mei=Yuta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakashibaShogo
en-aut-sei=Takashiba
en-aut-mei=Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=AriasZulema
en-aut-sei=Arias
en-aut-mei=Zulema
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of Pathophysiology – Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Pathophysiology – Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Pathophysiology – Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Pathophysiology – Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Research Core for Interdisciplinary Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Research Core for Interdisciplinary Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Pathophysiology – Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=bioactive sealer
kn-keyword=bioactive sealer
en-keyword=graphene oxide
kn-keyword=graphene oxide
en-keyword=mineral deposition
kn-keyword=mineral deposition
en-keyword=antimicrobial activity
kn-keyword=antimicrobial activity
en-keyword=radiopacity
kn-keyword=radiopacity
END

start-ver=1.4
cd-journal=joma
no-vol=76
cd-vols=
no-issue=6
article-no=
start-page=705
end-page=713
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=202212
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Impact of Tofogliflozin on Physiological and Hormonal Function, Serum Electrolytes, and Cardiac Diastolic Function in Elderly Japanese Patients with Type 2 Diabetes Mellitus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The sodium glucose transporter 2 (SGLT2) inhibitor tofogliflozin is a glucose-lowering drug that causes the excretion of surplus glucose by inhibiting SGLT2. Because of tofogliflozin’s osmotic diuresis mechanism, patients’ serum electrolytes, body fluid levels, and cardiac function must be monitored. We retrospectively analyzed the cases of 64 elderly Japanese patients with type 2 diabetes mellitus (T2DM) who received tofogliflozin for 3 months. Their HbA1c, serum electrolytes (sodium, potassium, chloride), hematocrit, brain natriuretic peptide (cardiac volume load marker) and renin and aldosterone (RAA; an index of regulatory hormones involved in body fluid retention) were continuously monitored during the investigation period. Renal function and cardiac function (by echocardiography) were assessed throughout the period. HbA1c significantly decreased (β1=−0.341, p<0.0001, linear regression analysis [LRA]). Most of the hormonal, electrolyte, and physiological parameters were maintained throughout the study period. In these circumstances, E/e’ tended to decrease (β1=−0.382, p=0.13, LRA). Compared to the baseline, E/e’ was significantly decreased at 1 and 3 months (p<0.01, p<0.05). In the higher E/e’ group (E/e’≥10, n=34), E/e’ decreased significantly (β1=−0.63, p<0.05, LRA). ΔE/e’ was correlated with body-weight change during treatment (r=0.64, p<0.01). The 3-month tofogliflozin treatment improved glycemic control and diastolic function represented by E/e’ in T2DM patients, without affecting serum electrolytes, renal function, or RAA. No negative impacts on the patients were observed. Three-month tofogliflozin treatment lowered glucose and improved cardiac diastolic function.
en-copyright=
kn-copyright=

en-aut-name=HigashikawaToshihiro
en-aut-sei=Higashikawa
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ItoTomohiko
en-aut-sei=Ito
en-aut-mei=Tomohiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MizunoTakurou
en-aut-sei=Mizuno
en-aut-mei=Takurou
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IshigamiKeiichiro
en-aut-sei=Ishigami
en-aut-mei=Keiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KurokiKengo
en-aut-sei=Kuroki
en-aut-mei=Kengo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MaekawaNaoto
en-aut-sei=Maekawa
en-aut-mei=Naoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=UsudaDaisuke
en-aut-sei=Usuda
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IzumidaToshihide
en-aut-sei=Izumida
en-aut-mei=Toshihide
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=YamadaShinya
en-aut-sei=Yamada
en-aut-mei=Shinya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=SangenRyusho
en-aut-sei=Sangen
en-aut-mei=Ryusho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=HamadaKazu
en-aut-sei=Hamada
en-aut-mei=Kazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=KiyosawaJun
en-aut-sei=Kiyosawa
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=SaitoAtsushi
en-aut-sei=Saito
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=IguchiMasaharu
en-aut-sei=Iguchi
en-aut-mei=Masaharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=KasamakiYuji
en-aut-sei=Kasamaki
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=NakahashiTakeshi
en-aut-sei=Nakahashi
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=FukudaAkihiro
en-aut-sei=Fukuda
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=SaitoHitoshi
en-aut-sei=Saito
en-aut-mei=Hitoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=KandaTsugiyasu
en-aut-sei=Kanda
en-aut-mei=Tsugiyasu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=OkuroMasashi
en-aut-sei=Okuro
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

affil-num=1
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=2
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=3
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=4
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=5
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=6
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=7
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=8
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=9
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=10
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=11
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=12
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=13
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=14
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=15
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=16
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=17
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=18
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=19
en-affil=Kanazawa Medical University Himi Municipal Hospital
kn-affil=

affil-num=20
en-affil=Department of Geriatric Medicine, Kanazawa Medical University
kn-affil=
en-keyword=tofogliflozin
kn-keyword=tofogliflozin
en-keyword=SGLT2 inhibitor
kn-keyword=SGLT2 inhibitor
en-keyword=elderly patient
kn-keyword=elderly patient
en-keyword=HbA1c
kn-keyword=HbA1c
en-keyword=cardiac diastolic function
kn-keyword=cardiac diastolic function
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=1
article-no=
start-page=15449
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220914
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of luseogliflozin and voglibose on high-risk lipid profiles and inflammatory markers in diabetes patients with heart failure
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Sodium-glucose cotransporter 2 inhibitors could reduce cardiovascular events in patients with heart failure irrespective of diabetes status. In this prespecified sub-analysis of randomised-controlled trial, we investigated the efficacy of luseogliflozin (2.5 mg daily), a sodium-glucose cotransporter 2 inhibitor, with that of voglibose (0.6 mg daily), an alpha-glucosidase inhibitor, on high-risk lipid profile and inflammatory markers in patients with type-2 diabetes and heart failure. Among the 157 patients studied, there were no significant differences in the mean malondialdehyde LDL or small-dense LDL cholesterol levels between the luseogliflozin and voglibose groups (percent change: 0.2% vs. - 0.6%, p = 0.93; - 1.7% vs. - 8.6%, p= 0.21) after 12 weeks in comparison to levels at the baseline. No significant difference was observed between the two groups in the adiponectin and high-sensitivity C-reactive protein levels after 12 weeks compared to the baseline levels (percent change, - 1.6% vs. - 4.0% and 22.5% vs. 10.0%; p = 0.52 and p = 0.55, respectively). In conclusion, in patients with type-2 diabetes and heart failure, compared to voglibose, luseogliflozin did not significantly improve the high-risk lipoprotein profile including malondialdehyde LDL and small-dense LDL cholesterol or the levels of inflammatory markers, including adiponectin and high-sensitivity C-reactive protein.
en-copyright=
kn-copyright=

en-aut-name=EjiriKentaro
en-aut-sei=Ejiri
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KiharaHajime
en-aut-sei=Kihara
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HataYoshiki
en-aut-sei=Hata
en-aut-mei=Yoshiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NaganoToshihiko
en-aut-sei=Nagano
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakaishiAtsushi
en-aut-sei=Takaishi
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TodaHironobu
en-aut-sei=Toda
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NambaSeiji
en-aut-sei=Namba
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NakamuraYoichi
en-aut-sei=Nakamura
en-aut-mei=Yoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=AkagiSatoshi
en-aut-sei=Akagi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SakuragiSatoru
en-aut-sei=Sakuragi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=MinagawaTaro
en-aut-sei=Minagawa
en-aut-mei=Taro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KawaiYusuke
en-aut-sei=Kawai
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=NishiiNobuhiro
en-aut-sei=Nishii
en-aut-mei=Nobuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=FukeSoichiro
en-aut-sei=Fuke
en-aut-mei=Soichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=YoshikawaMasaki
en-aut-sei=Yoshikawa
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=The MUSCAT-HF Study  Investigators
en-aut-sei=The MUSCAT-HF Study  Investigators
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and  Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and  Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Internal Medicine, Kihara Cardiovascular Clinic
kn-affil=

affil-num=4
en-affil=Department of Cardiology, Minamino Cardiovascular Hospital
kn-affil=

affil-num=5
en-affil=Department  of Internal Medicine, Iwasa Hospital
kn-affil=

affil-num=6
en-affil=Department of Cardiology, Mitoyo General Hospital
kn-affil=

affil-num=7
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and  Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department  of Cardiology, Okayama Rosai Hospital
kn-affil=

affil-num=9
en-affil=Department of Cardiovascular Medicine, Specifed  Clinic of Soyokaze Cardiovascular Medicine and Diabetes Care
kn-affil=

affil-num=10
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and  Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Cardiovascular Medicine,  Iwakuni Clinical Center
kn-affil=

affil-num=12
en-affil=Department of Internal Medicine, Minagawa Cardiovascular Clinic
kn-affil=

affil-num=13
en-affil=Department of Cardiovascular Medicine, Okayama City Hospital
kn-affil=

affil-num=14
en-affil=Department  of Internal Medicine, Yoshinaga Hospital
kn-affil=

affil-num=15
en-affil=Department of Cardiovascular Medicine, Japanese Red  Cross Okayama Hospital
kn-affil=

affil-num=16
en-affil=Department of Cardiology, Fukuyama City Hospital
kn-affil=

affil-num=17
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and  Pharmaceutical Sciences
kn-affil=

affil-num=18
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and  Pharmaceutical Sciences
kn-affil=

affil-num=19
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=23
cd-vols=
no-issue=7
article-no=
start-page=3587
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Pathophysiology and Treatment of Diabetic Cardiomyopathy and Heart Failure in Patients with Diabetes Mellitus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=There is a close relationship between diabetes mellitus and heart failure, and diabetes is an independent risk factor for heart failure. Diabetes and heart failure are linked by not only the complication of ischemic heart disease, but also by metabolic disorders such as glucose toxicity and lipotoxicity based on insulin resistance. Cardiac dysfunction in the absence of coronary artery disease, hypertension, and valvular disease is called diabetic cardiomyopathy. Diabetes-induced hyperglycemia and hyperinsulinemia lead to capillary damage, myocardial fibrosis, and myocardial hypertrophy with mitochondrial dysfunction. Lipotoxicity with extensive fat deposits or lipid droplets is observed on cardiomyocytes. Furthermore, increased oxidative stress and inflammation cause cardiac fibrosis and hypertrophy. Treatment with a sodium glucose cotransporter 2 (SGLT2) inhibitor is currently one of the most effective treatments for heart failure associated with diabetes. However, an effective treatment for lipotoxicity of the myocardium has not yet been established, and the establishment of an effective treatment is needed in the future. This review provides an overview of heart failure in diabetic patients for the clinical practice of clinicians.
en-copyright=
kn-copyright=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YoshidaMasashi
en-aut-sei=Yoshida
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AkagiSatoshi
en-aut-sei=Akagi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SaitoYukihiro
en-aut-sei=Saito
en-aut-mei=Yukihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=EjiriKentaro
en-aut-sei=Ejiri
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MatsuoNaoaki
en-aut-sei=Matsuo
en-aut-mei=Naoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IchikawaKeishi
en-aut-sei=Ichikawa
en-aut-mei=Keishi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=IwasakiKeiichiro
en-aut-sei=Iwasaki
en-aut-mei=Keiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=NaitoTakanori
en-aut-sei=Naito
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NambaYusuke
en-aut-sei=Namba
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=YoshidaMasatoki
en-aut-sei=Yoshida
en-aut-mei=Masatoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=SugiyamaHiroki
en-aut-sei=Sugiyama
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=9
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=10
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=11
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=12
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=13
en-affil=Department of Internal Medicine, Okayama Saiseikai General Hospital
kn-affil=

affil-num=14
en-affil=Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=heart failure
kn-keyword=heart failure
en-keyword=lipotoxicity
kn-keyword=lipotoxicity
en-keyword=SGLT2 inhibitor
kn-keyword=SGLT2 inhibitor
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20211103
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of luseogliflozin on estimated plasma volume in patients with heart failure with preserved ejection fraction
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Aims<br>
Sodium glucose co-transporter 2 inhibitors have diuretic effects in both patients with glycosuria and with natriuresis. We sought to assess the effect of luseogliflozin on estimated plasma volume (ePV) in patients with type 2 diabetes and heart failure with preserved ejection fraction (HFpEF). <br>
Methods and results<br>
This study was a post-hoc analysis of the MUSCAT-HF trial (UMIN000018395), a multicentre, prospective, open-label, randomized controlled trial that assessed the effect of 12 weeks of luseogliflozin (2.5 mg, once daily, n = 83) as compared with voglibose (0.2 mg, three times daily, n = 82) on the reduction in brain natriuretic peptide (BNP) in patients with type 2 diabetes and HFpEF. The analysis compared the change in ePV calculated by the Straus formula from baseline to Weeks 4, 12, and 24, using a mixed-effects model for repeated measures. We also estimated the association between changes in ePV and changes in other clinical parameters, including BNP levels. Luseogliflozin significantly reduced ePV as compared to voglibose at Week 4 {adjusted mean group-difference -6.43% [95% confidence interval (CI): -9.11 to -3.74]}, at Week 12 [-8.73% (95%CI: -11.40 to -6.05)], and at Week 24 [-11.02% (95%CI: -13.71 to -8.33)]. The effect of luseogliflozin on these parameters was mostly consistent across various patient clinical characteristics. The change in ePV at Week 12 was significantly associated with log-transformed BNP (r = 0.197, P = 0.015) and left atrial volume index (r = 0.283, P = 0.019). <br>
Conclusions<br>
Luseogliflozin significantly reduced ePV in patients with type 2 diabetes and HFpEF, as compared with voglibose. The reduction of intravascular volume by luseogliflozin may provide clinical benefits to patients with type 2 diabetes and HFpEF.
en-copyright=
kn-copyright=

en-aut-name=NakashimaMitsutaka
en-aut-sei=Nakashima
en-aut-mei=Mitsutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=EjiriKentaro
en-aut-sei=Ejiri
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KiharaHajime
en-aut-sei=Kihara
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HataYoshiki
en-aut-sei=Hata
en-aut-mei=Yoshiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NaganoToshihiko
en-aut-sei=Nagano
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakaishiAtsushi
en-aut-sei=Takaishi
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TodaHironobu
en-aut-sei=Toda
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NanbaSeiji
en-aut-sei=Nanba
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=NakamuraYoichi
en-aut-sei=Nakamura
en-aut-mei=Yoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=AkagiSatoshi
en-aut-sei=Akagi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=SakuragiSatoru
en-aut-sei=Sakuragi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=MinagawaTaro
en-aut-sei=Minagawa
en-aut-mei=Taro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=KawaiYusuke
en-aut-sei=Kawai
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=NishiiNobuhiro
en-aut-sei=Nishii
en-aut-mei=Nobuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=FukeSoichiro
en-aut-sei=Fuke
en-aut-mei=Soichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=YoshikawaMasaki
en-aut-sei=Yoshikawa
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=MUSCAT-HF Study Investigators
en-aut-sei=MUSCAT-HF Study Investigators
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Internal Medicine, Kihara Cardiovascular Clinic
kn-affil=

affil-num=5
en-affil=Department of Cardiology, Minamino Cardiovascular Hospital
kn-affil=

affil-num=6
en-affil=Department of Internal Medicine, Iwasa Hospital,
kn-affil=

affil-num=7
en-affil=Department of Cardiology, Mitoyo General Hospital
kn-affil=

affil-num=8
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Cardiology, Okayama Rosai Hospital
kn-affil=

affil-num=10
en-affil=Department of Cardiovascular Medicine, Specified Clinic of Soyokaze CardioVascular Medicine and Diabetes Care, Matsuyama
kn-affil=

affil-num=11
en-affil=Department of Internal Medicine, Akaiwa Medical Association Hospital
kn-affil=

affil-num=12
en-affil=Department of Cardiovascular Medicine, Iwakuni Clinical Center
kn-affil=

affil-num=13
en-affil=Department of Internal Medicine, Minagawa Cardiovascular Clinic
kn-affil=

affil-num=14
en-affil=Department of Cardiovascular Medicine, Okayama City Hospital
kn-affil=

affil-num=15
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=16
en-affil=Department of Cardiovascular Medicine, Japanese Red Cross Okayama Hospital
kn-affil=

affil-num=17
en-affil=Department of Cardiology, Fukuyama City Hospital
kn-affil=

affil-num=18
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=19
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=20
en-affil=
kn-affil=
en-keyword=Estimated plasma volume
kn-keyword=Estimated plasma volume
en-keyword=Heart failure with preserved ejection fraction
kn-keyword=Heart failure with preserved ejection fraction
en-keyword=Luseogliflozin
kn-keyword=Luseogliflozin
en-keyword=Sodium glucose co-transporter 2 inhibitors
kn-keyword=Sodium glucose co-transporter 2 inhibitors
en-keyword=Voglibose
kn-keyword=Voglibose
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=14
article-no=
start-page=L140402
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=2021107
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Spin-gap formation due to spin-Peierls instability in π-orbital-ordered NaO2
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We have investigated the low-temperature magnetism of sodium superoxide (NaO2), in which spin, orbital, and lattice degrees of freedom are closely entangled. The magnetic susceptibility shows anomalies at T1 = 220 K and T2 = 190 K, which correspond well to the structural phase transition temperatures, and a sudden decrease below T3 = 34 K. At 4.2 K, the magnetization shows a clear stepwise anomaly around 30 T with a large hysteresis. In addition, the muon spin relaxation experiments indicate no magnetic phase transition down to T = 0.3 K. The inelastic neutron scattering spectrum exhibits magnetic excitation with a finite energy gap. These results confirm that the ground state of NaO2 is a spin-singlet state. To understand this ground state in NaO2, we performed Raman scattering experiments. All the Raman-active libration modes expected for the marcasite phase below T2 are observed. Furthermore, we find that several new peaks appear below T3. This directly evidences the low crystal symmetry, namely, the presence of the phase transition at T3.We conclude that the singlet ground state of NaO2 is due to the spin-Peierls instability.
en-copyright=
kn-copyright=

en-aut-name=MiyajimaMizuki
en-aut-sei=Miyajima
en-aut-mei=Mizuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AstutiFahmi
en-aut-sei=Astuti
en-aut-mei=Fahmi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FukudaTakahito
en-aut-sei=Fukuda
en-aut-mei=Takahito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KodaniMasashi
en-aut-sei=Kodani
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=IidaShinsuke
en-aut-sei=Iida
en-aut-mei=Shinsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=AsaiShinichiro
en-aut-sei=Asai
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MatsuoAkira
en-aut-sei=Matsuo
en-aut-mei=Akira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MasudaTakatsugu
en-aut-sei=Masuda
en-aut-mei=Takatsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KindoKoichi
en-aut-sei=Kindo
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HasegawaTakumi
en-aut-sei=Hasegawa
en-aut-mei=Takumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KobayashiTatsuo C.
en-aut-sei=Kobayashi
en-aut-mei=Tatsuo C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=NakanoTakehito
en-aut-sei=Nakano
en-aut-mei=Takehito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=WatanabeIsao
en-aut-sei=Watanabe
en-aut-mei=Isao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=KambeTakashi
en-aut-sei=Kambe
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

affil-num=1
en-affil=Department of Physics, Okayama University
kn-affil=

affil-num=2
en-affil=Advanced Meson Science Laboratory, RIKEN Nishina Center
kn-affil=

affil-num=3
en-affil=Department of Physics, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Physics, Okayama University
kn-affil=

affil-num=5
en-affil=Institute for Solid State Physics, University of Tokyo
kn-affil=

affil-num=6
en-affil=Institute for Solid State Physics, University of Tokyo
kn-affil=

affil-num=7
en-affil=Institute for Solid State Physics, University of Tokyo
kn-affil=

affil-num=8
en-affil=Institute for Solid State Physics, University of Tokyo
kn-affil=

affil-num=9
en-affil=Institute for Solid State Physics, University of Tokyo
kn-affil=

affil-num=10
en-affil=Graduate School of Advanced Science and Engineering, Hiroshima University
kn-affil=

affil-num=11
en-affil=Department of Physics, Okayama University
kn-affil=

affil-num=12
en-affil=Institute of Quantum Beam Science, Ibaraki University
kn-affil=

affil-num=13
en-affil=Advanced Meson Science Laboratory, RIKEN Nishina Center
kn-affil=

affil-num=14
en-affil=Department of Physics, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=171
cd-vols=
no-issue=
article-no=
start-page=106777
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202112
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Portable two-color photometer based on paired light emitter detector diodes and its application to the determination of paraquat and diquat
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Here we describe a methodology for the determination of paraquat and diquat using a newly developed portable photometer equipped with two colors of paired light emitter detector diodes (PEDD). The colorimetric measurements employed in this work include the redox reactions between 1) dithiothreitol and diquat to produce the red color characteristic of a diquat radical and 2) between sodium dithionite and either diquat or paraquat that results in the green and blue colors of diquat and paraquat radicals, respectively. The addition of sodium dithionite or dithiothreitol in a solid-state provides reproducible absorbance of the radicals, prevents decomposition of the reagents in a solution, and simplifies handling of the reagents. The diquat radical produced by dithiothreitol (λmax = 495 nm) was successfully detected by using a pair of blue LEDs with a maximum emission wavelength at 472 nm while the radicals of paraquat (λmax = 603 nm) and diquat (λmax = 771 nm) reduced by sodium dithionite were measured by a pair of orange LEDs with a maximum emission wavelength of 609 nm. The proposed method consists of measuring diquat radicals at 472 nm, estimating the absorbance of diquat radicals at 609 nm, and subtracting the estimated absorbance of diquat radicals from the total absorbance at 609 nm to determine paraquat radicals. The developed method yielded examples of excellent linear regression (r2) of more than 0.99 in three calibration curves of the radicals measured at 472 nm for diquat radicals and measured at 609 nm for both diquat and paraquat radicals. The intra-day (n = 3) and inter-day (n = 3) precision of three calibration curves were less than or equal to 5%. By comparison with the standard method of high-performance liquid chromatography, the reliability of the proposed method was proven via the analysis of paraquat and diquat radicals in a commercially available herbicide.
en-copyright=
kn-copyright=

en-aut-name=SeetasangSasikarn
en-aut-sei=Seetasang
en-aut-mei=Sasikarn
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Diquat
kn-keyword=Diquat
en-keyword=Dithiothreitol
kn-keyword=Dithiothreitol
en-keyword=Light-emitting diode
kn-keyword=Light-emitting diode
en-keyword=Paraquat
kn-keyword=Paraquat
en-keyword=Photometric detector
kn-keyword=Photometric detector
en-keyword=Sodium dithionite
kn-keyword=Sodium dithionite
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=1
article-no=
start-page=287
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210823
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Overexpression of SGLT2 in the kidney of a P. gingivalis LPS-induced diabetic nephropathy mouse model
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: The overexpression of sodium-glucose cotransporter 2 (SGLT2) in diabetic kidneys has been reported. It has also been established that the diabetic glomerular endothelium expresses the toll-like receptors TLR2 and TLR4. The present study aims to examine the renal SGLT2 induction by the TLR2/4 ligand Porphyromonas (P.) gingivalis lipopolysaccharide (Pg-LPS) in mouse diabetic nephropathy. <br>
Methods: Immunohistochemical study and tissue RT-PCR analyses were performed on mouse kidneys in streptozotocin (STZ)-induced diabetic ICR mice (STZ-ICR), in healthy ICR mice administered Pg-LPS (LPS-ICR), and in diabetic ICR mouse kidneys with Pg-LPS-induced nephropathy (LPS-STZ). <br>
Results: In the quantitative analysis of blood sugar levels, the mean time to reach 600 mg/dl was shorter in the LPS-STZ than in the STZ-ICR kidneys. The rise in blood glucose levels was significantly steeper in the LPS-STZ than in the STZ-ICR kidneys. According to these data the LPS-STZ model suggests a marked glucose intolerance. The expression of SGLT2 was significantly stronger in the whole of the renal parenchyma of the LPS-STZ than in the LPS-ICR or in the STZ-ICR. The expression of SGLT2 was observed both in the renal tubules and around the renal tubules, and in the glomeruli of the LPS-STZ kidneys. In the analysis by tissue real-time PCR and cell ELISA, the expression of the SGLT2 gene and protein was significantly stronger in the LPS-STZ than in the LPS-ICR or in the STZ-ICR. There were no differences in the renal SGLT2 production in the LPS-ICR and the STZ-ICR kidneys. <br>
Conclusions: Abnormally high renal expression of SGLT2 occurs in diabetic kidneys with P. gingivalis LPS. Periodontitis may be an exacerbating factor in diabetic nephropathy as well as in diabetes.
en-copyright=
kn-copyright=

en-aut-name=KajiwaraKoichiro
en-aut-sei=Kajiwara
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SawaYoshihiko
en-aut-sei=Sawa
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Department of Oral Growth & Development, Fukuoka Dental College
kn-affil=

affil-num=2
en-affil=Department of Oral Function & Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=P. gingivalis
kn-keyword=P. gingivalis
en-keyword=LPS
kn-keyword=LPS
en-keyword=Diabetic nephropathy
kn-keyword=Diabetic nephropathy
en-keyword=SGLT2
kn-keyword=SGLT2
END

start-ver=1.4
cd-journal=joma
no-vol=125
cd-vols=
no-issue=23
article-no=
start-page=6296
end-page=6305
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202168
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Ion Size Dependences of the Salting-Out Effect: Reversed Order of Sodium and Lithium Ions
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A general trend of the salting-out effect on hydrophobic solutes in aqueous solution is that the smaller the size of a dissolved ion, the larger the effect of reducing the solubility of a hydrophobe. An exception is that Li+, the smallest in alkali metal ions, has a notably weaker effect than Na+. To understand the reversed order in the cation series, we performed molecular dynamics simulations of aqueous solutions of salt ions and calculated the Setschenow coefficient of methane with the ionic radius of either a cation or an anion varied in a wide range. It is confirmed that the Setschenow coefficient is correlated with the packing fraction of salt solution, as observed in earlier studies, and also correlated with the partial molar volume of an ion. Analyses of correlation function integrals, packing fractions of solvation spheres, and orientations of water molecules surrounding an ion reveal the key differences in microscopic properties between the cation and anion series, which give rise to the reversed order in the cation series of the partial molar volumes of ions and ultimately that of the Setschenow coefficients.
en-copyright=
kn-copyright=

en-aut-name=KatsutoHiroyuki
en-aut-sei=Katsuto
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkamotoRyuichi
en-aut-sei=Okamoto
en-aut-mei=Ryuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SumiTomonari
en-aut-sei=Sumi
en-aut-mei=Tomonari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KogaKenichiro
en-aut-sei=Koga
en-aut-mei=Kenichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=

affil-num=2
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=

affil-num=3
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=

affil-num=4
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=7
cd-vols=
no-issue=2
article-no=
start-page=55
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210406
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Anti-Inflammatory Effect on Colitis and Modulation of Microbiota by Fermented Plant Extract Supplementation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Although results of recent studies suggest that fermented foods strongly affect the gut microbiota composition and that they relieve inflammatory bowel disease symptoms, some reports have described that fermented foods increase some inflammation markers based on differences in fermented food materials. This study evaluated the effects of fermented plant extract (FPE) on dextran sulfate sodium (DSS)-induced colitis in mice and the effects on fecal microbiota composition in humans. Mice fed 5% FPE with 3% DSS (FPE group) showed no body weight loss, atrophy of colonic length, or bloody stool, similar to mice fed a basal diet (negative group), whereas mice fed 3% DSS (positive group) exhibited those effects. Concentrations of inflammation markers IL-6 and TNF-alpha were not significantly different between FPE and negative groups; however, those concentrations became higher in the positive group. 16S ribosomal RNA gene sequencing was used to characterize fecal microbiota in healthy women before and after 3-month FPE supplementation. The FPE supplementation induced increases in Firmicutes phyla and in Clostridiales order, which play a central role in inflammation suppression. These results suggest that FPE enhances Clostridiales growth in the gut and that it has an anti-inflammatory effect.
en-copyright=
kn-copyright=

en-aut-name=SugimotoManabu
en-aut-sei=Sugimoto
en-aut-mei=Manabu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WatanabeToshiro
en-aut-sei=Watanabe
en-aut-mei=Toshiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakaokaMotoko
en-aut-sei=Takaoka
en-aut-mei=Motoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SuzukiKyoko
en-aut-sei=Suzuki
en-aut-mei=Kyoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MurakamiTadatoshi
en-aut-sei=Murakami
en-aut-mei=Tadatoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MurakamiNobutada
en-aut-sei=Murakami
en-aut-mei=Nobutada
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SumikawaShoichi
en-aut-sei=Sumikawa
en-aut-mei=Shoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Food and Nutrition, Sonoda Women’s University
kn-affil=

affil-num=3
en-affil=Department of Biosphere Sciences, Kobe College
kn-affil=

affil-num=4
en-affil=Department of Biosphere Sciences, Kobe College
kn-affil=

affil-num=5
en-affil=Functional Food Creation Research Institute Co., Ltd.
kn-affil=

affil-num=6
en-affil=Functional Food Creation Research Institute Co., Ltd.
kn-affil=

affil-num=7
en-affil=Functional Food Creation Research Institute Co., Ltd.
kn-affil=
en-keyword=fermented plant extract
kn-keyword=fermented plant extract
en-keyword=microbiota
kn-keyword=microbiota
en-keyword=dextran sulfate sodium
kn-keyword=dextran sulfate sodium
en-keyword=inflammatory
kn-keyword=inflammatory
en-keyword=Clostridiales
kn-keyword=Clostridiales
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=11
article-no=
start-page=5582
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210525
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Management of Cirrhotic Ascites under the Add-on Administration of Tolvaptan
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Tolvaptan is a recently available diuretic that blocks arginine vasopressin receptor 2 in the renal collecting duct. Its diuretic mechanism involves selective water reabsorption by affecting the water reabsorption receptor aquaporin 2. Given that liver cirrhosis patients exhibit hyponatremia due to their pseudo-aldosteronism and usage of natriuretic agents, a sodium maintaining agent, such as tolvaptan, is physiologically preferable. However, large scale studies indicating the patients for whom this would be effective and describing management under its use have been insufficient. The appropriate management of cirrhosis patients treated with tolvaptan should be investigated. In the present review, we collected articles investigating the effectiveness of tolvaptan and factors associated with survival and summarized their management reports. Earlier administration of tolvaptan before increasing the doses of natriuretic agents is recommended because this may preserve effective arterial blood volume.
en-copyright=
kn-copyright=

en-aut-name=AdachiTakuya
en-aut-sei=Adachi
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakeuchiYasuto
en-aut-sei=Takeuchi
en-aut-mei=Yasuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakakiAkinobu
en-aut-sei=Takaki
en-aut-mei=Akinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OyamaAtsushi
en-aut-sei=Oyama
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WadaNozomu
en-aut-sei=Wada
en-aut-mei=Nozomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OnishiHideki
en-aut-sei=Onishi
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=ShirahaHidenori
en-aut-sei=Shiraha
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Department of Gastroenterology and Hepatology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=tolvaptan
kn-keyword=tolvaptan
en-keyword=liver cirrhosis
kn-keyword=liver cirrhosis
en-keyword=ascites
kn-keyword=ascites
END

start-ver=1.4
cd-journal=joma
no-vol=4
cd-vols=
no-issue=1
article-no=
start-page=76
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210524
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Halogen-sodium exchange enables efficient access to organosodium compounds
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With sodium being the most abundant alkali metal on Earth, organosodium compounds are an attractive choice for sustainable chemical synthesis. However, organosodium compounds are rarely used-and are overshadowed by organolithium compounds-because of a lack of convenient and efficient preparation methods. Here we report a halogen-sodium exchange method to prepare a large variety of (hetero)aryl- and alkenylsodium compounds including tri- and tetrasodioarenes, many of them previously inaccessible by other methods. The key discovery is the use of a primary and bulky alkylsodium lacking beta-hydrogens, which retards undesired reactions, such as Wurtz-Fittig coupling and beta-hydrogen elimination, and enables efficient halogen-sodium exchange. The alkylsodium is readily prepared in situ from neopentyl chloride and an easy-to-handle sodium dispersion. We believe that the efficiency, generality, and convenience of the present method will contribute to the widespread use of organosodium in organic synthesis, ultimately contributing to the development of sustainable organic synthesis by rivalling the currently dominant organolithium reagents. Halogen-sodium exchange reactions with neopentyl sodium provides access to a range of aryl and alkenyl organosodium compounds in situ, as an alternative to organolithium reagents.
en-copyright=
kn-copyright=

en-aut-name=AsakoSobi
en-aut-sei=Asako
en-aut-mei=Sobi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakahashiIkko
en-aut-sei=Takahashi
en-aut-mei=Ikko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakajimaHirotaka
en-aut-sei=Nakajima
en-aut-mei=Hirotaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IliesLaurean
en-aut-sei=Ilies
en-aut-mei=Laurean
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakaiKazuhiko
en-aut-sei=Takai
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=RIKEN Center for Sustainable Resource Science
kn-affil=

affil-num=3
en-affil=Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=4
en-affil=RIKEN Center for Sustainable Resource Science
kn-affil=

affil-num=5
en-affil=Division of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=SGLT2阻害薬は肥満マウスの近位尿細管細胞のオートファジー障害を改善する
kn-title=Sodium Glucose Co-Transporter 2 Inhibitor Ameliorates Autophagic Flux Impairment on Renal Proximal Tubular Cells in Obesity Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=FukushimaKazuhiko
en-aut-sei=Fukushima
en-aut-mei=Kazuhiko
kn-aut-name=福島和彦
kn-aut-sei=福島
kn-aut-mei=和彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=1
article-no=
start-page=103
end-page=107
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202102
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Marked Hypertriglyceridemia in a Patient with type 2 Diabetes Receiving SGLT2 Inhibitors
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A 43-year-old male with type 2 diabetes, under treatment with 5 mg/day of dapagliflozin, was referred to our hospital with upper left abdominal pain and marked hypertriglyceridemia (triglycerides [TGs], 5,960 mg/dl). He was also on a low-carbohydrate diet that promoted ketosis under sodium glucose cotransporter 2 (SGLT2) inhibitor administration. Polyacrylamide gel electrophoresis revealed a remarkable increase in very-low-den-sity lipoprotein, a TG-rich lipoprotein particle synthesized in the liver using free fatty acids derived from adi-pose tissue. Although SGLT2 inhibitors generally improve the lipid profile, under certain conditions such as a low-carbohydrate diet, they may adversely exacerbate the lipid profile via ketosis.
en-copyright=
kn-copyright=

en-aut-name=SenooMayumi
en-aut-sei=Senoo
en-aut-mei=Mayumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ToneAtsuhito
en-aut-sei=Tone
en-aut-mei=Atsuhito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ImaiYusuke
en-aut-sei=Imai
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WatanabeSatoko
en-aut-sei=Watanabe
en-aut-mei=Satoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KanetoMitsuhiro
en-aut-sei=Kaneto
en-aut-mei=Mitsuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ShimomuraYasuyuki
en-aut-sei=Shimomura
en-aut-mei=Yasuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TeshigawaraSanae
en-aut-sei=Teshigawara
en-aut-mei=Sanae
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NakatouTatsuaki
en-aut-sei=Nakatou
en-aut-mei=Tatsuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=2
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=3
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=4
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=5
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=6
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=7
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=

affil-num=8
en-affil=Department of Internal Medicine, Diabetes Center, Okayama Saiseikai General Hospital
kn-affil=
en-keyword=sodium glucose cotransporter 2 inhibitor
kn-keyword=sodium glucose cotransporter 2 inhibitor
en-keyword=dyslipidemia
kn-keyword=dyslipidemia
en-keyword=hypertriglyceridemia
kn-keyword=hypertriglyceridemia
en-keyword=type 2 diabetes mellitus
kn-keyword=type 2 diabetes mellitus
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=1
article-no=
start-page=63
end-page=69
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202102
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Optimal Prepregnancy Body Mass Index for Lactation in Japanese Women with Neonatal Separation as Analyzed by a Differential Equation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We used a differential equation to identify the biological relationship between the maternal prepregnancy body mass index (BMI) and lactation on postpartum day 4 in Japanese women with neonatal separation. This retro-spective observational study included 252 mothers (135 primiparas, 117 multiparas) whose singleton neonates were admitted to a neonatal ICU. We formulated hypotheses based on breast anatomy to analyze the relation-ship between the expressed milk obtained on postpartum day 4 and the maternal prepregnancy BMI with the following differential equation: y’(x) = k y(x)/x, where k is the constant, x is the prepregnancy BMI, and y is the expressed milk volume. The formula was then obtained as y(x) = axk, where a is the constant. The Akaike information criterion (AIC) was used to estimate the regression equation with the maximum likelihood for primiparas and multiparas. The best criteria for BMI determined by the AIC were 20.89 kg/m2 in primiparas and 20.19 kg/m2 in multiparas. These were the optimal BMI values for lactation, coinciding with the median prepregnancy BMI in the study population (20.78 kg/m2 in primiparas and 20.06 kg/m2 in multiparas). The formula based on biomathematics might help establish the biological relationship between prepregnancy BMI and breastmilk volume.
en-copyright=
kn-copyright=

en-aut-name=TadaKatsuhiko
en-aut-sei=Tada
en-aut-mei=Katsuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyagiYasunari
en-aut-sei=Miyagi
en-aut-mei=Yasunari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakamuraKazue
en-aut-sei=Nakamura
en-aut-mei=Kazue
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YorozuMoe
en-aut-sei=Yorozu
en-aut-mei=Moe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=FukushimaEmi
en-aut-sei=Fukushima
en-aut-mei=Emi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KumazawaKazumasa
en-aut-sei=Kumazawa
en-aut-mei=Kazumasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NakamuraMakoto
en-aut-sei=Nakamura
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KageyamaMisao
en-aut-sei=Kageyama
en-aut-mei=Misao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Obstetrics and Gynecology, National Hospital Organization, Okayama Medical Center
kn-affil=

affil-num=2
en-affil=Miyake Ofuku Clinic
kn-affil=

affil-num=3
en-affil=Department of Neonatology, National Hospital Organization, Okayama Medical Center
kn-affil=

affil-num=4
en-affil=Department of Obstetrics and Gynecology, National Hospital Organization, Okayama Medical Center
kn-affil=

affil-num=5
en-affil=Department of Nursing, National Hospital Organization, Okayama Medical Center
kn-affil=

affil-num=6
en-affil=Department of Obstetrics and Gynecology, National Hospital Organization, Okayama Medical Center
kn-affil=

affil-num=7
en-affil=Department of Neonatology, National Hospital Organization, Okayama Medical Center
kn-affil=

affil-num=8
en-affil=Department of Neonatology, National Hospital Organization, Okayama Medical Center
kn-affil=
en-keyword=biomathematics
kn-keyword=biomathematics
en-keyword=body mass index
kn-keyword=body mass index
en-keyword=expressed milk
kn-keyword=expressed milk
en-keyword=lactation
kn-keyword=lactation
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=1
article-no=
start-page=45
end-page=53
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202102
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Possible Protective Effect of Remote Ischemic Preconditioning on Acute Kidney Injury Following Elective Percutaneous Coronary Intervention: Secondary Analysis of a Multicenter, Randomized Study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Remote ischemic preconditioning (RIPC) is a promising strategy for protecting against ischemic reperfusion injury. This study is a secondary analysis of a randomized study that aimed to evaluate the effect of RIPC on the early increase in serum creatinine (SCr) following percutaneous coronary intervention (PCI), which is associ-ated with contrast-induced acute kidney injury. Patients with stable angina undergoing elective PCI were assigned to control, RIPC, and continuous infusion of nicorandil (nicorandil) groups. The endpoint of this study was the incidence of the early increase in SCr, a predictor of contrast-induced acute kidney injury, which was defined as either a > 20% or absolute increase by 0.3 mg/dl of SCr levels after 24 h of PCI. This study included 220 patients for whom a dataset of SCr values was available. The incidence of the early increase in SCr was significantly lower in the RIPC than in the control (1.3% vs 10.8%, p = 0.03) group, but was not significantly different between the nicorandil and control groups. In multivariate analysis, RIPC remained a significant fac-tor associated with a reduction in the incidence of early increase in SCr. RIPC reduces the incidence of early increase in SCr in patients with stable angina following elective PCI.
en-copyright=
kn-copyright=

en-aut-name=OtsukaHiroaki
en-aut-sei=Otsuka
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=EjiriKentaro
en-aut-sei=Ejiri
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KohnoKunihisa
en-aut-sei=Kohno
en-aut-mei=Kunihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NakahamaMakoto
en-aut-sei=Nakahama
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=DoiMasayuki
en-aut-sei=Doi
en-aut-mei=Masayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MunemasaMitsuru
en-aut-sei=Munemasa
en-aut-mei=Mitsuru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MurakamiMasaaki
en-aut-sei=Murakami
en-aut-mei=Masaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Cardiology, Fukuyama City Hospital
kn-affil=

affil-num=6
en-affil=Department of Cardiology, Kagawa Prefectural Central Hospital
kn-affil=

affil-num=7
en-affil=Department of Cardiology, Okayama Medical Center
kn-affil=

affil-num=8
en-affil=Department of Cardiology, Okayama Heart Clinic
kn-affil=

affil-num=9
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=
en-keyword=remote ischemic preconditioning
kn-keyword=remote ischemic preconditioning
en-keyword=stable angina
kn-keyword=stable angina
en-keyword=serum creatinine
kn-keyword=serum creatinine
en-keyword=acute kidney injury
kn-keyword=acute kidney injury
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=16
article-no=
start-page=e015103
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200818
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of Luseogliflozin on Heart Failure With Preserved Ejection Fraction in Patients With Diabetes Mellitus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background</br>
Effects of sodium‐glucose cotransporter 2 inhibitors on reducing hospitalization for heart failure have been reported in randomized controlled trials, but their effects on patients with heart failure with preserved ejection fraction (HFpEF) are unknown. This study aimed to evaluate the drug efficacy of luseogliflozin, a sodium‐glucose cotransporter 2 inhibitor, in patients with type 2 diabetes mellitus and HFpEF.</br>
Methods and Results</br>
We performed a multicenter, open‐label, randomized, controlled trial for comparing luseogliflozin 2.5 mg once daily with voglibose 0.2 mg 3 times daily in patients with type 2 diabetes mellitus suffering from HFpEF (left ventricular ejection fraction >45% and BNP [B‐type natriuretic peptide] concentrations ≥35 pg/mL) in a 1:1 randomization fashion. The primary outcome was the difference from baseline in BNP levels after 12 weeks of treatment between the 2 drugs. A total of 173 patients with diabetes mellitus and HFpEF were included. Of these, 83 patients were assigned to receive luseogliflozin and 82 to receive voglibose. There was no significant difference in the reduction in BNP concentrations after 12 weeks from baseline between the 2 groups. The ratio of the mean BNP value at week 12 to the baseline value was 0.79 in the luseogliflozin group and 0.87 in the voglibose group (percent change, −9.0% versus −1.9%; ratio of change with luseogliflozin versus voglibose, 0.93; 95% CI, 0.78–1.10; P=0.26).</br>
Conclusion</br>
In patients with type 2 diabetes mellitus and HFpEF, there is no significant difference in the degree of reduction in BNP concentrations after 12 weeks between luseogliflozin and voglibose.
en-copyright=
kn-copyright=

en-aut-name=EjiriKentaro
en-aut-sei=Ejiri
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyoshiToru
en-aut-sei=Miyoshi
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KiharaHajime
en-aut-sei=Kihara
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HataYoshiki
en-aut-sei=Hata
en-aut-mei=Yoshiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NaganoToshihiko
en-aut-sei=Nagano
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakaishiAtsushi
en-aut-sei=Takaishi
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TodaHironobu
en-aut-sei=Toda
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=NanbaSeiji
en-aut-sei=Nanba
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NakamuraYoichi
en-aut-sei=Nakamura
en-aut-mei=Yoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=AkagiSatoshi
en-aut-sei=Akagi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=SakuragiSatoru
en-aut-sei=Sakuragi
en-aut-mei=Satoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=MinagawaTaro
en-aut-sei=Minagawa
en-aut-mei=Taro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=KawaiYusuke
en-aut-sei=Kawai
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=NishiiNobuhiro
en-aut-sei=Nishii
en-aut-mei=Nobuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=FukeSoichiro
en-aut-sei=Fuke
en-aut-mei=Soichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=YoshikawaMasaki
en-aut-sei=Yoshikawa
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

affil-num=1
en-affil=Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Internal Medicine, Kihara Cardiovascular Clinic
kn-affil=

affil-num=4
en-affil=Department of Cardiology, Minamino Cardiovascular Hospital
kn-affil=

affil-num=5
en-affil=Department of Internal Medicine, Iwasa Hospital
kn-affil=

affil-num=6
en-affil=Department of Cardiology, Mitoyo General Hospital
kn-affil=

affil-num=7
en-affil=Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Cardiology, Okayama Rosai Hospital
kn-affil=

affil-num=9
en-affil=Department of Cardiovascular Medicine, Specified Clinic of Soyokaze Cardiovascular Medicine and Diabetes Care
kn-affil=

affil-num=10
en-affil=Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Cardiovascular Medicine, Iwakuni Clinical Center
kn-affil=

affil-num=12
en-affil=Department of Internal Medicine, Minagawa Cardiovascular Clinic
kn-affil=

affil-num=13
en-affil=Department of Cardiovascular Medicine, Okayama City Hospital
kn-affil=

affil-num=14
en-affil=Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=15
en-affil=Department of Cardiovascular Medicine, Japanese Red Cross Okayama Hospital
kn-affil=

affil-num=16
en-affil=Department of Cardiology, Fukuyama City Hospital
kn-affil=

affil-num=17
en-affil=Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=

affil-num=18
en-affil=Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences
kn-affil=
en-keyword=B-type natriuretic peptide
kn-keyword=B-type natriuretic peptide
en-keyword=diabetes mellitus
kn-keyword=diabetes mellitus
en-keyword=heart failure
kn-keyword=heart failure
en-keyword=sodium-glucose cotransporter 2 inhibitor
kn-keyword=sodium-glucose cotransporter 2 inhibitor
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=42
article-no=
start-page=21780
end-page=21787
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200928
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Iron nanoparticle templates for constructing 3D graphene framework with enhanced performance in sodium-ion batteries
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study examines the synthesis and electrochemical performance of three-dimensional graphene for Li-ion batteries and Na-ion batteries. The in situ formation of iron hydroxide nanoparticles (Fe(OH)x NPs) of various weights on the surface of graphene oxide, followed by thermal treatment at elevated temperature and washing using hydrochloric acid, furnished 3D graphene. The characterization studies confirmed the prevention of graphene layer stacking by over 90% compared with thermal treatment without Fe(OH)x. The electrochemical performance of the 3D graphene was evaluated as a counter electrode for lithium metal and sodium metal in a half-cell configuration. This material showed good performances with a charging capacity of 507 mA h g−1 at 372 mA g−1 in Li-ion batteries and 252 mA h g−1 at 100 mA g−1 in Na-ion batteries, which is 1.4 and 1.9 times higher, respectively, than the graphene prepared without Fe(OH)x templates.
en-copyright=
kn-copyright=

en-aut-name=CampéonBenoît D. L.
en-aut-sei=Campéon
en-aut-mei=Benoît D. L.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WangChen
en-aut-sei=Wang
en-aut-mei=Chen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NishinaYuta
en-aut-sei=Nishina
en-aut-mei=Yuta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Research Core for Interdisciplinary Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200925
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=慢性腎臓病患者における複数回随時尿と24時間尿のナトリウム・カリウム比の関連性の検討
kn-title=The relationship between repeated measurement of casual and 24-h urinary sodium-to-potassium ratio in patients with chronic kidney disease
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=OkuyamaYuka
en-aut-sei=Okuyama
en-aut-mei=Yuka
kn-aut-name=奥山由加
kn-aut-sei=奥山
kn-aut-mei=由加
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=10
article-no=
start-page=2149
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200923
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=In Vitro Studies to Define the Cell-Surface and Intracellular Targets of Polyarginine-Conjugated Sodium Borocaptate as a Potential Delivery Agent for Boron Neutron Capture Therapy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Boron neutron capture therapy (BNCT) requires pharmaceutical innovations and molecular-based evidence of effectiveness to become a standard cancer therapeutic in the future. Recently, in Japan, 4-borono-L-phenylalanine (BPA) was approved as a boron agent for BNCT against head and neck (H&N) cancers. H&N cancer appears to be a suitable target for BPA-BNCT, because the expression levels of L-type amino acid transporter 1 (LAT1), one of the amino acid transporters responsible for BPA uptake, are elevated in most cases of H&N cancer. However, in other types of cancer including malignant brain tumors, LAT1 is not always highly expressed. To expand the possibility of BNCT for these cases, we previously developed poly-arginine peptide (polyR)-conjugated mercaptoundecahydrododecaborate (BSH). PolyR confers the cell membrane permeability and tumor selectivity of BSH. However, the molecular determinants for the properties are not fully understood. In this present study, we have identified the cluster of differentiation 44 (CD44) protein and translational machinery proteins as a major cell surface target and intracellular targets of BSH-polyR, respectively. CD44, also known as a stem cell-associated maker in various types of cancer, is required for the cellular uptake of polyR-conjugated molecules. We showed that BSH-polyR was predominantly delivered to a CD44(High) cell population of cancer cells. Once delivered, BSH-polyR interacted with the translational machinery components, including the initiation factors, termination factors, and poly(A)-biding protein (PABP). As a proof of principle, we performed BSH-polyR-based BNCT against glioma stem-like cells and revealed that BSH-polyR successfully induced BNCT-dependent cell death specifically in CD44(High) cells. Bioinformatics analysis indicated that BSH-polyR would be suitable for certain types of malignant tumors. Our results shed light on the biochemical properties of BSH-polyR, which may further contribute to the therapeutic optimization of BSH-BNCT in the future.
en-copyright=
kn-copyright=

en-aut-name=FujimuraAtsushi
en-aut-sei=Fujimura
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YasuiSeiji
en-aut-sei=Yasui
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=IgawaKazuyo
en-aut-sei=Igawa
en-aut-mei=Kazuyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=UedaAi
en-aut-sei=Ueda
en-aut-mei=Ai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WatanabeKaori
en-aut-sei=Watanabe
en-aut-mei=Kaori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HanafusaTadashi
en-aut-sei=Hanafusa
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=IchikawaYasuaki
en-aut-sei=Ichikawa
en-aut-mei=Yasuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YoshihashiSachiko
en-aut-sei=Yoshihashi
en-aut-mei=Sachiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TsuchidaKazuki
en-aut-sei=Tsuchida
en-aut-mei=Kazuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KamiyaAtsunori
en-aut-sei=Kamiya
en-aut-mei=Atsunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=FuruyaShuichi
en-aut-sei=Furuya
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=

affil-num=3
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=

affil-num=4
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=

affil-num=5
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=

affil-num=6
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=

affil-num=7
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=

affil-num=8
en-affil=Graduate School of Engineering, Nagoya University
kn-affil=

affil-num=9
en-affil=Graduate School of Engineering, Nagoya University
kn-affil=

affil-num=10
en-affil=Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Neutron Therapy Research Center, Okayama University
kn-affil=
en-keyword=boron neutron capture therapy (BNCT)
kn-keyword=boron neutron capture therapy (BNCT)
en-keyword=BSH-polyR
kn-keyword=BSH-polyR
en-keyword=CD44
kn-keyword=CD44
en-keyword=translational machinery
kn-keyword=translational machinery
en-keyword=bioinformatics
kn-keyword=bioinformatics
END

start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=3
article-no=
start-page=188
end-page=196
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=2020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.
en-copyright=
kn-copyright=

en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KatsumataEmiko
en-aut-sei=Katsumata
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MizoteAkiko
en-aut-sei=Mizote
en-aut-mei=Akiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=JianHou Jian
en-aut-sei=Jian
en-aut-mei=Hou Jian
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MuhomahTeresia Aluoch
en-aut-sei=Muhomah
en-aut-mei=Teresia Aluoch
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Hayashibara Co., Ltd.
kn-affil=

affil-num=4
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=6
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=oligosaccharide
kn-keyword=oligosaccharide
en-keyword=DSS-induced colitis
kn-keyword=DSS-induced colitis
en-keyword=goblet cell
kn-keyword=goblet cell
en-keyword=gut microbiota
kn-keyword=gut microbiota
en-keyword=immunoglobulin A
kn-keyword=immunoglobulin A
END

start-ver=1.4
cd-journal=joma
no-vol=1135
cd-vols=
no-issue=
article-no=
start-page=99
end-page=106
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200827
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=On-site analysis of paraquat using a completely portable photometric detector operated with small, rechargeable batteries
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This work describes a methodology that can be used to achieve on-site analysis of paraquat in water samples by using a miniaturized portable photometer consisting of a couple of light-emitting diodes (LEDs). Paraquat produces a colored radical via a redox reaction with sodium dithionite, which is unstable against oxygen in solution. The steps taken to stabilize the reagent solution included control of the pH and the addition of organic solvents, but the most effective was the formation of an oil layer. Together, these steps stabilized the reagent solution for two days. An increase in the duration of reagent stability, however, is necessary in order to transport the reagent for on-site applications in remote locales. For the time being, an excess amount of solid sodium dithionite can be added directly to sample solutions because the unreacted dithionite shows no influence on absorbance of the paraquat radical. Orange LEDs with a maximum emission wavelength of 609 nm were employed in the portable photometer to measure the absorbance of paraquat radical produced by a redox reaction that has an absorption maximum of 603 nm. The developed photometer showed excellent performance with a linear range of from 2.0 mg L−1 to 40.0 mg L−1 and a linear regression (r2 = 1). The limits of detection and quantification were 0.5 mg L−1 and 1.5 mg L−1, respectively, intra-day precision (n = 3) and inter-day precision (n = 5) were both less than 5%, and accuracy based on the percentage of sample recovery ranged from 89 ± 0 to 105 ± 0% (n = 3). The proposed method was applied to the analysis of paraquat in water samples taken from rice fields. The results showed no paraquat in all thirteen samples, which could have been due to strong adsorption of paraquat by soil particles and/or to complications with the sampling conditions. To confirm the adsorption onto soil of paraquat contained in water, we constructed an artificial rice field where water containing paraquat was impounded above the soil layer. The results showed that paraquat in water gradually decreased within three days and could be measured in the soil on the fourth day. These results were confirmed by HPLC analysis, which underscores the utility of this portable photometer for the on-site monitoring of paraquat in water samples.
en-copyright=
kn-copyright=

en-aut-name=SeetasangSasikarn
en-aut-sei=Seetasang
en-aut-mei=Sasikarn
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Light-emitting diode
kn-keyword=Light-emitting diode
en-keyword=Paraquat
kn-keyword=Paraquat
en-keyword=Portable photometric detector
kn-keyword=Portable photometric detector
en-keyword=Rice field
kn-keyword=Rice field
en-keyword=Sodium dithionite
kn-keyword=Sodium dithionite
en-keyword=Thailand
kn-keyword=Thailand
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=4
article-no=
start-page=319
end-page=325
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=202008
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Relationship Between Partial Carbon Dioxide Pressure and Strong Ions in Humans: A Retrospective Study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Little is known about the role of a strong ions in humans with respiratory abnormalities. In this study, we investigated the associations between partial carbon dioxide pressure (pCO2) and each of sodium ion (Na+) concentrations, chloride ion (Cl−) concentrations and their difference (SIDNa-Cl). Blood gas data were obtained from patients in a teaching hospital intensive care unit between August 2013 and January 2017. The association between pCO2 and SIDNa-Cl was defined as the primary outcome. The associations between pCO2 and [Cl−], [Na+] and other strong ions were secondary outcomes. pCO2 was stratified into 10 mmHg-wide bands and treated as a categorical variable for comparison. As a result, we reviewed 115,936 blood gas data points from 3,840 different ICU stays. There were significant differences in SIDNa-Cl, [Cl−], and [Na+] among all categorized pCO2 bands. The respective pCO2 SIDNa-Cl, [Cl−], and [Na+] correlation coefficients were 0.48, −0.31, and 0.08. SIDNa-Cl increased and [Cl−] decreased with pCO2, with little relationship between pCO2 and [Na+] across subsets. In conclusion, we found relatively strong correlations between pCO2 and SIDNa-Cl in the multiple blood gas datasets examined. Correlations between pCO2 and chloride concentrations, but not sodium concentrations, were further found to be moderate in these ICU data.
en-copyright=
kn-copyright=

en-aut-name=IsoyamaSatoshi
en-aut-sei=Isoyama
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KimuraSatoshi
en-aut-sei=Kimura
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MorimatsuHiroshi
en-aut-sei=Morimatsu
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Department of Anesthesiology and Resuscitation, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Anesthesiology and Resuscitation, Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Department of Anesthesiology and Resuscitation, Okayama University Hospital
kn-affil=
en-keyword=acid-base phenomena
kn-keyword=acid-base phenomena
en-keyword=Stewart approach
kn-keyword=Stewart approach
en-keyword=strong ion difference
kn-keyword=strong ion difference
en-keyword=chlorine ion
kn-keyword=chlorine ion
en-keyword=partial carbon dioxide pressure
kn-keyword=partial carbon dioxide pressure
END

start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=29
article-no=
start-page=14472
end-page=14481
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200519
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Mechanisms for overcharging of carbon electrodes in lithium-ion/sodium-ion batteries analysed by operando solid-state NMR
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A precise understanding of the mechanism for metal (Li and Na) plating on negative electrodes that occurs with overcharging is critical to managing the safety of lithium- and sodium-ion batteries. In this work, an in-depth investigation of the overlithiation/oversodiation and subsequent delithiation/desodiation of graphite and hard carbon electrodes in the first cycle was conducted using operando7Li/23Na solid-state NMR. In the 7Li NMR spectra of half cells of carbon electrodes and metal counter electrodes, three types of signals corresponding to Li dendrites that formed on the surface of graphite, hard carbon, and the counter electrode were distinguished from the signal of Li metal foil of the counter electrode by applying an appropriate orientation of the testing cell. For graphite overlithiation, the deposition of Li dendrites started immediately or soon after the minimum electric potential in the lithiation curve. In contrast, the deposition of Li dendrites in hard carbon started after the end of quasimetallic lithium formation for overlithiation at rates below 3.0C. Similar behaviour was also observed for the oversodiation of hard carbon. The formation of quasimetallic Li or Na in the pores of hard carbon serves as a buffer for the metal plating that occurs with overcharging of the batteries. Furthermore, some of the deposited Li/Na dendrites contribute to reversible capacities. A mechanism for the inhomogeneous disappearance of quasimetallic Li during delithiation of hard carbon is also proposed.
en-copyright=
kn-copyright=

en-aut-name=GotohKazuma
en-aut-sei=Gotoh
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamakamiTomu
en-aut-sei=Yamakami
en-aut-mei=Tomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NishimuraIshin
en-aut-sei=Nishimura
en-aut-mei=Ishin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KometaniHina
en-aut-sei=Kometani
en-aut-mei=Hina
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=AndoHideka
en-aut-sei=Ando
en-aut-mei=Hideka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HashiKenjiro
en-aut-sei=Hashi
en-aut-mei=Kenjiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=ShimizuTadashi
en-aut-sei=Shimizu
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IshidaHiroyuki
en-aut-sei=Ishida
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=

affil-num=6
en-affil=National Institute for Materials Science
kn-affil=

affil-num=7
en-affil=National Institute for Materials Science
kn-affil=

affil-num=8
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=9
article-no=
start-page=2470
end-page=2479
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=2014029
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Microfluidic Approach to the Formation of Internally Porous Polymer Particles by Solvent Extraction
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We report the controlled formation of internally porous polyelectrolyte particles with diameters ranging from tens to hundreds of micrometers through selective solvent extraction using microfluidics. Solvent-resistant microdevices, fabricated by frontal photopolymerization, encapsulate binary polymer (P)/solvent (S1) mixtures by a carrier solvent phase (C) to form plugs with well-defined radii and low polydispersity; the suspension is then brought into contact with a selective extraction solvent (S2) that is miscible with C and S1 but not P, leading to the extraction of S1 from the droplets. The ensuing phase inversion yields polymer capsules with a smooth surface but highly porous internal structure. Depending on the liquid extraction time scale, this stage can be carried out in situ, within the chip, or ex situ, in an external S2 bath. Bimodal polymer plugs are achieved using asymmetrically inverted T junctions. For this demonstration, we form sodium poly(styrenesulfonate) (P) particles using water (S1), hexadecane (C), and methyl ethyl ketone (S2). We measure droplet extraction rates as a function of drop size and polymer concentration and propose a simple scaling model to guide particle formation. We find that the extraction time required to form particles from liquid droplets does not depend on the initial polymer concentration but is rather proportional to the initial droplet size. The resulting particle size follows a linear relationship with the initial droplet size for all polymer concentrations, allowing for the precise control of particle size. The internal particle porous structure exhibits a polymer density gradient ranging from a dense surface skin toward an essentially hollow core. Average particle porosities between 10 and 50% are achieved by varying the initial droplet compositions up to 15 wt % polymer. Such particles have potential applications in functional, optical, and coating materials.
en-copyright=
kn-copyright=

en-aut-name=WatanabeTakaichi
en-aut-sei=Watanabe
en-aut-mei=Takaichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=LopezCarlos G.
en-aut-sei=Lopez
en-aut-mei=Carlos G.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=DouglasJack F.
en-aut-sei=Douglas
en-aut-mei=Jack F.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OnoTsutomu
en-aut-sei=Ono
en-aut-mei=Tsutomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=CabralJoão T.
en-aut-sei=Cabral
en-aut-mei=João T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Department of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil= Department of Chemical Engineering, Imperial College London
kn-affil=

affil-num=3
en-affil= Materials Science and Engineering Division, National Institute of Standards and Technology
kn-affil=

affil-num=4
en-affil=Department of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=5
en-affil=Department of Chemical Engineering, Imperial College London
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=21
cd-vols=
no-issue=11
article-no=
start-page=4054
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200605
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Sodium Glucose Co-Transporter 2 Inhibitor Ameliorates Autophagic Flux Impairment on Renal Proximal Tubular Cells in Obesity Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Obesity is supposed to cause renal injury via autophagy deficiency. Recently, sodium glucose co-transporter 2 inhibitors (SGLT2i) were reported to protect renal injury. However, the mechanisms of SGLT2i for renal protection are unclear. Here, we investigated the effect of SGLT2i for autophagy in renal proximal tubular cells (PTCs) on obesity mice. We fed C57BL/6J mice with a normal diet (ND) or high-fat and -sugar diet (HFSD) for nine weeks, then administered SGLT2i, empagliflozin, or control compound for one week. Each group contained N = 5. The urinary N-acetyl-beta-d-glucosaminidase level in the HFSD group significantly increased compared to ND group. The tubular damage was suppressed in the SGLT2i-HFSD group. In electron microscopic analysis, multi lamellar bodies that increased in autophagy deficiency were increased in PTCs in the HFSD group but significantly suppressed in the SGLT2i group. The autophagosomes of damaged mitochondria in PTCs in the HFSD group frequently appeared in the SGLT2i group. p62 accumulations in PTCs were significantly increased in HFSD group but significantly suppressed by SGLT2i. In addition, the mammalian target of rapamycin was activated in the HFSD group but significantly suppressed in SGLT2i group. These data suggest that SGLT2i has renal protective effects against obesity via improving autophagy flux impairment in PTCs on a HFSD.
en-copyright=
kn-copyright=

en-aut-name=FukushimaKazuhiko
en-aut-sei=Fukushima
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KitamuraShinji
en-aut-sei=Kitamura
en-aut-mei=Shinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TsujiKenji
en-aut-sei=Tsuji
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SangYizhen
en-aut-sei=Sang
en-aut-mei=Yizhen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=sodium glucose co-transporter 2 inhibitor
kn-keyword=sodium glucose co-transporter 2 inhibitor
en-keyword=mammalian target of rapamycin (mTOR)
kn-keyword=mammalian target of rapamycin (mTOR)
en-keyword=autophagy
kn-keyword=autophagy
en-keyword=obesity
kn-keyword=obesity
en-keyword=multi lamellar body
kn-keyword=multi lamellar body
END

start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=1
article-no=
start-page=77
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191125
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Anesthetic management of a patient with sodium-channel myotonia: a case report
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Sodium-channel myotonia (SCM) is a nondystrophic myotonia, characterized by pure myotonia without muscle weakness or paramyotonia. The prevalence of skeletal muscle channelopathies is approximately 1 in 100,000, and the prevalence of SCM is much lower. To our knowledge, this is the first report on anesthetic management of a patient with SCM. </br>
Case presentation: A 23-year-old woman with congenital nasal dysplasia and SCM was scheduled to undergo rhinoplasty with autologous costal cartilage. Total intravenous anesthesia without muscle relaxants was administered followed by continuous intercostal nerve block. Although transient elevation of potassium level in the blood was observed during surgery, the patient did not show exacerbation of myotonic or paralytic symptoms in the postoperative period. </br>
Conclusion: Total intravenous anesthesia and peripheral nerve block can be administered safely to a patient with SCM. However, careful monitoring of the symptoms and electrolytes is recommended.
en-copyright=
kn-copyright=

en-aut-name=MatsumotoNaohisa
en-aut-sei=Matsumoto
en-aut-mei=Naohisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NishimotoRei
en-aut-sei=Nishimoto
en-aut-mei=Rei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MatsuokaYoshikazu
en-aut-sei=Matsuoka
en-aut-mei=Yoshikazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakedaYoshimasa
en-aut-sei=Takeda
en-aut-mei=Yoshimasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MorimatsuHiroshi
en-aut-sei=Morimatsu
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Department of Anesthesiology and Resuscitology, Okayama University Hospital
kn-affil=

affil-num=2
en-affil=Department of Anesthesiology and Resuscitology, Okayama University Hospital
kn-affil=

affil-num=3
en-affil=Departments of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine
kn-affil=

affil-num=4
en-affil=Department of Anesthesiology and Resuscitology, Okayama University Hospital
kn-affil=

affil-num=5
en-affil=Department of Anesthesiology and Resuscitology, Okayama University Hospital
kn-affil=
en-keyword=Anesthetic management
kn-keyword=Anesthetic management
en-keyword=Myotonia congenita
kn-keyword=Myotonia congenita
en-keyword=Nondystrophic myotonia
kn-keyword=Nondystrophic myotonia
en-keyword=Paramyotonia congenita
kn-keyword=Paramyotonia congenita
en-keyword=Periodic paralysis
kn-keyword=Periodic paralysis
en-keyword=Potassium-aggravated myotonia
kn-keyword=Potassium-aggravated myotonia
en-keyword=Skeletal muscle channelopathy
kn-keyword=Skeletal muscle channelopathy
en-keyword=Sodium-channel myotonia
kn-keyword=Sodium-channel myotonia
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=12
article-no=
start-page=125317
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191220
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Magnetic characterization change by solvents of magnetic nanoparticles in liquid-phase magnetic immunoassay
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Liquid-phase magnetic immunoassay (MIA) using magnetic nano-particles (MNPs) has been studied as a more rapid method compared to optical methods for inspecting proteins and viruses. MIA can estimate the number of conjugated antibodies without being washed differently from conventional optical immunoassay. However, in the case of the liquid phase, it is considered that the magnetic properties of MNPs are affected by physical properties such as viscosity and impurity substances such as biological substances contained in the blood. In this study, the effect of sodium chloride (NaCl) in buffer and serum solution was evaluated to reveal the effect of serum because the sodium (Na+) and chloride (Cl-) ions in the serum dominate ion balance of blood. The measurement results of AC magnetic susceptibility and a dynamic light scattering (DLS) showed that the aggregation of MNPs was largely affected by the concentration of NaCl. This effect of the NaCl could be explained by shielding of the surface charge of MNPs by ions in the solution. Although the concentrations of NaCl in the buffer and serum solution were almost same, we found that MNPs were aggregated more in their size for those in the serum solution because of other impurities, such as proteins. These results suggest evaluation of effects of the contaminants in serum and optimization of polymer coatings of MNPs could be important factors to realize measurements of magnetic immunoassay with high accuracy. (C) 2019 Author(s).
en-copyright=
kn-copyright=

en-aut-name=JinnoKatsuya
en-aut-sei=Jinno
en-aut-mei=Katsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HiramatsuBunta
en-aut-sei=Hiramatsu
en-aut-mei=Bunta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TsunashimaKenta
en-aut-sei=Tsunashima
en-aut-mei=Kenta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FujimotoKayo
en-aut-sei=Fujimoto
en-aut-mei=Kayo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SakaiKenji
en-aut-sei=Sakai
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KiwaToshihiko
en-aut-sei=Kiwa
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TsukadaKeiji
en-aut-sei=Tsukada
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=

affil-num=7
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=4
article-no=
start-page=551
end-page=560
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190923
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Separation Between Silicon and Aluminum Powders Contained Within Pulverized Scraped Silicon-Based Waste Solar Cells by Flotation Method
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= There are few study examples on the separation of metals by floating method. In this study, separation of silicon and aluminum, which are the main components of silicon-based solar cell module, was carried out by floating method in order to purify silicon from waste solar cell module. The selection of surfactant, control of electric charge, wettability of the solid particles, surface tensions, and bubble surface area are important for separation of solids by floating method. Sodium dodecyl sulfate (SDS) can increase the hydrophobicity of aluminum powder due to the difference of surface potentials between silicon and aluminum. SDS behaves as a collector of aluminum as well as a frothing agent to decrease the bubble size. At a SDS concentration of 2 g/L and sample dipping time of 10 min, 80.1 mass% of aluminum was floated and separated, and the sedimentary silicon reached a purity of 90.7% from a mixture of 50 mass% aluminum and 50 mass% silicon. Finally, at a pH value of 7.0, SDS concentration between 1.0 and 2.5 g/L and air flow rate of 2.5 L/min (STP) were suitable experimental conditions to purify silicon from a mixture of silicon and aluminum by flotation separation method.
en-copyright=
kn-copyright=

en-aut-name=HaradaSho
en-aut-sei=Harada
en-aut-mei=Sho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UddinMd. Azhar
en-aut-sei=Uddin
en-aut-mei=Md. Azhar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatoYoshiei
en-aut-sei=Kato
en-aut-mei=Yoshiei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KawanishiTakanori
en-aut-sei=Kawanishi
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HayashiYoshiaki
en-aut-sei=Hayashi
en-aut-mei=Yoshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Department of Material and Energy Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Material and Energy Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Material and Energy Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Wet Process Division, Toho Kasei Co., Ltd
kn-affil=

affil-num=5
en-affil=Wet Process Division, Toho Kasei Co., Ltd
kn-affil=
en-keyword=Flotation
kn-keyword=Flotation
en-keyword=Floating separation
kn-keyword=Floating separation
en-keyword=Waste solar cell module
kn-keyword=Waste solar cell module
en-keyword=Silicon
kn-keyword=Silicon
en-keyword=Sodium dodecyl sulfate
kn-keyword=Sodium dodecyl sulfate
END

start-ver=1.4
cd-journal=joma
no-vol=83
cd-vols=
no-issue=5
article-no=
start-page=914
end-page=922
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190217
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation. Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks. After 3 weeks, the mice received water containing 5% (w/v) DSS or not for 1 week. The disease activity index score in mice fed MFP was significantly decreased. A significant decrease in Bifidobacterium spp. and the Clostridium perfringens subgroup was observed in mice not fed MFP. The number of goblet cell and NLRP6 expression were observed in mice fed a diet supplemented with MFP compared with mice not fed MFP. These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.
en-copyright=
kn-copyright=

en-aut-name=WangYang
en-aut-sei=Wang
en-aut-mei=Yang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HatabuToshimitsu
en-aut-sei=Hatabu
en-aut-mei=Toshimitsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science , Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Environmental and Life Science , Okayama University
kn-affil=
en-keyword=DSS-induced acute colitis
kn-keyword=DSS-induced acute colitis
en-keyword=Mulberry
kn-keyword=Mulberry
en-keyword=NLRP6 inflammasome
kn-keyword=NLRP6 inflammasome
en-keyword=goblet cell
kn-keyword=goblet cell
en-keyword=microbiota
kn-keyword=microbiota
END

start-ver=1.4
cd-journal=joma
no-vol=49
cd-vols=
no-issue=3
article-no=
start-page=521
end-page=526
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=20071121
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Rasmussen encephalitis associated with SCN1A mutation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Mutations in the SCN 1 A gene, encoding the neuronal voltage-gated sodium channel alpha1 subunit, cause SMEI, GEFS+, and related epileptic syndromes. We herein report the R1575C-SCN 1 A mutation identified in a patient with Rasmussen encephalitis. R1575C were constructed in a recombinant human SCN 1 A and then heterologously expressed in HEK293 cells along with the human beta1 and beta2 sodium channel accessory subunits. Whole-cell patch-clamp recording was used to define biophysical properties. The R1575C channels exhibited increased channel availability and an increased persistent sodium current in comparison to the wild-type. These defects of electrophysiological properties can result in neuronal hyperexitability. The seizure susceptibility allele may influence the pathogenesis of Rasmussen encephalitis in this case.
en-copyright=
kn-copyright=

en-aut-name=OhmoriIori
en-aut-sei=Ohmori
en-aut-mei=Iori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OuchidaMamoru
en-aut-sei=Ouchida
en-aut-mei=Mamoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KobayashiKatsuhiro
en-aut-sei=Kobayashi
en-aut-mei=Katsuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=JitsumoriYoshimi
en-aut-sei=Jitsumori
en-aut-mei=Yoshimi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=InoueTakushi
en-aut-sei=Inoue
en-aut-mei=Takushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ShimizuKenji
en-aut-sei=Shimizu
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MatsuiHideki
en-aut-sei=Matsui
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OhtsukaYoko
en-aut-sei=Ohtsuka
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=MaegakiYoshihiro
en-aut-sei=Maegaki
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Departments of Cellular Physiology, Graduate School of Medicine,Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Departments of Molecular Genetics, Graduate School of Medicine,Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Departments of Child Neurology, Graduate School of Medicine,Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=
kn-affil=

affil-num=5
en-affil=Departments of Child Neurology, Graduate School of Medicine,Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Departments of Molecular Genetics, Graduate School of Medicine,Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Departments of Cellular Physiology, Graduate School of Medicine,Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Departments of Child Neurology, Graduate School of Medicine,Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=9
en-affil=Division of Child Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University
kn-affil=
en-keyword=Rasmussen encephalitis
kn-keyword=Rasmussen encephalitis
en-keyword=SCN1A
kn-keyword=SCN1A
en-keyword=genetic-environmental interaction
kn-keyword=genetic-environmental interaction
END

start-ver=1.4
cd-journal=joma
no-vol=4
cd-vols=
no-issue=12
article-no=
start-page=15249
end-page=15254
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190703
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Characterization of Pieces of Paper That Form Reagent Containers for Use as Portable Analytical Devices
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Reagent-deposited pieces of paper were characterized by the use of a compact conductometer, a compact pH sensor, and a conventional spectrophotometer to assess their suitability for use as reagent containers. The pieces of paper were fabricated by wax printing to form a limited hydrophilic area to which a consistent volume of an aqueous reagent could be added. The pieces of paper without the reagent increased the conductivity of water gradually because of the release of sodium salts, whereas pH of NaOH decreased because of the acidity of the functional groups in the paper. Three reagents, sulfamic acid as an acid, Na2CO3 as a base, and BaCl2 as a metal salt, were deposited on the pieces of paper to evaluate their ability to release from the pieces of paper. Sulfamic acid and Na2CO3 were released in quantities of 58 and 73% into water after 420 s, whereas 100% of BaCl2 was released after 480 s. The conductometric titrations of NaOH, HCl, and Na2SO4, and the spectrophotometry of Fe2+ were examined using the pieces of paper that contained sulfamic acid, Na2CO3, BaCl2, and 1,10-phenanthroline. Titrations using the pieces of paper suggested that the reagents were quantitatively released into the titrant, which resulted in a linear relationship between the endpoints and the equivalent points. In 120 s of soaking time, 60-70% of the reagents were released. The spectrophotometric measurements of Fe2+ indicated that when an excess amount of the reagents was deposited onto the pieces of paper, they nonetheless sufficiently fulfilled the role of a reagent container.
en-copyright=
kn-copyright=

en-aut-name=BukingSupatana
en-aut-sei=Buking
en-aut-mei=Supatana
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SuedomiYusuke
en-aut-sei=Suedomi
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NacaprichaDuangjai
en-aut-sei=Nacapricha
en-aut-mei=Duangjai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs) and Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University
kn-affil=

affil-num=2
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil=Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs) and Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University
kn-affil=

affil-num=4
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=50
cd-vols=
no-issue=
article-no=
start-page=209
end-page=217
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201302
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=CACNA1A variants may modify the epileptic phenotype of Dravet syndrome
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Dravet syndrome is an intractable epileptic syndrome beginning in the first year of life. De novo mutations of SCN1A, which encode the Na(v)1.1 neuronal voltage-gated sodium channel, are considered the major cause of Dravet syndrome. In this study, we investigated genetic modifiers of this syndrome. We performed a mutational analysis of all coding exons of CACNA1A in 48 subjects with Dravet syndrome. To assess the effects of CACNA1A variants on the epileptic phenotypes of Dravet syndrome, we compared clinical features in two genotype groups: 1) subjects harboring SCN1A mutations but no CACNA1A variants (n=20) and 2) subjects with SCN1A mutations plus CACNA1A variants (n=20). CACNA1A variants detected in patients were studied using heterologous expression of recombinant human Ca(v)2.1 in HEK 293 cells and whole-cell patch-clamp recording. Nine CACNA1A variants, including six novel ones, were detected in 21 of the 48 subjects (43.8%). Based on the incidence of variants in healthy controls, most of the variants seemed to be common polymorphisms. However, the subjects harboring SCN1A mutations and CACNA1A variants had absence seizures more frequently than the patients with only SCN1A mutations (8/20 vs. 0/20, p=0.002). Moreover, the former group of subjects exhibited earlier onset of seizures and more frequent prolonged seizures before one year of age, compared to the latter group of subjects. The electrophysiological properties of four of the five novel Ca(v)2.1 variants exhibited biophysical changes consistent with gain-of-function. We conclude that CACNA1A variants in some persons with Dravet syndrome may modify the epileptic phenotypes.
en-copyright=
kn-copyright=

en-aut-name=OhmoriIori
en-aut-sei=Ohmori
en-aut-mei=Iori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OuchidaMamoru
en-aut-sei=Ouchida
en-aut-mei=Mamoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KobayashiKatsuhiro
en-aut-sei=Kobayashi
en-aut-mei=Katsuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=JitsumoriYoshimi
en-aut-sei=Jitsumori
en-aut-mei=Yoshimi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MoriAkiko
en-aut-sei=Mori
en-aut-mei=Akiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MichiueHiroyuki
en-aut-sei=Michiue
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NishikiTeiichi
en-aut-sei=Nishiki
en-aut-mei=Teiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OhtsukaYoko
en-aut-sei=Ohtsuka
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=MatsuiHideki
en-aut-sei=Matsui
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Molecular Genetics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=
kn-affil=

affil-num=4
en-affil=
kn-affil=

affil-num=5
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=
kn-affil=

affil-num=9
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=2
article-no=
start-page=127
end-page=133
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=201904
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Thyroid Function Decline and Diet in Female High School Long-distance Runners
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= We aimed to clarify the state of thyroid function in female high school long-distance runners. We evaluated the associations between thyroid function and menstrual condition, bone mineral density (BMD), nutritious status, and body composition. The subjects’ height and weight were measured, along with fat percentage, fat mass, muscle mass, and BMD with dual-energy X-ray absorptiometry. A nutrition and dietary survey measured the subjects’ intake of energy and nutrients based on meals provided at the subjects’ dorm for 3 days in July of 2016 and 2017. Blood parameters including thyroid hormone and estradiol were measured. Most of the subjects (81.3%) were underweight (body mass index <18.5). The thyroid hormone free T3 value was decreased, but TSH was not increased and was similar to that observed in individuals with anorexia nervosa. In our subjects, thyroid hormone was associated with BMD and nutritional intake. To improve the menstruation abnormality of female athletes and to increase their bone density, the athletes’ weight should be managed by proper nutrient intake and the maintenance of their thyroid function.
en-copyright=
kn-copyright=

en-aut-name=IwasakiYukari
en-aut-sei=Iwasaki
en-aut-mei=Yukari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyaharaKimiko
en-aut-sei=Miyahara
en-aut-mei=Kimiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MiyatakeNobuyuki
en-aut-sei=Miyatake
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NakatsukaMikiya
en-aut-sei=Nakatsuka
en-aut-mei=Mikiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Health Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Faculty of Health Care, Department of Nutrition, Kiryu University
kn-affil=

affil-num=3
en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=4
en-affil=Graduate School of Health Sciences, Okayama University
kn-affil=
en-keyword=thyroid function
kn-keyword=thyroid function
en-keyword=nutritious status
kn-keyword=nutritious status
en-keyword=female high school long-distance runners
kn-keyword=female high school long-distance runners
en-keyword=bone mineral density
kn-keyword=bone mineral density
en-keyword=menstrual condition
kn-keyword=menstrual condition
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=6
article-no=
start-page=553
end-page=562
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=201812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Changes in Serum Biochemical Markers in Relation to Chief Complaints and Aging in General Medicine
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= To clarify potential relationships between chief complaints of patients and laboratory data with a focus on aging-related changes, we retrospectively analyzed the data of 843 patients who visited a general medicine department for the first time. Their chief complaints were classified into 8 major symptoms: visceral pain, somatic pain, fever, cough, dizziness, fatigue, appetite loss, and edema. We compared the laboratory data obtained from the patients with complaints with the data of symptom-free (control) patients. The serum sodium and potassium levels in the fever group were decreased compared to those in the control group. In the fever group, the serum sodium level was inversely correlated with age. The ratio of serum urea nitrogen to creatinine (UN/Cr) was increased in the appetite-loss group. There were significant age-dependent increases in the UN/Cr ratio in the appetite-loss and edema groups. Of note, serum levels of free thyroxin were lower in the dizziness group compared to the control group. In addition, the free thyroxin level was inversely correlated with age in the dizziness group but not in the asymptomatic control group. Collectively, the results indicated that osmolality-related laboratory data are strongly associated with individual primary symptoms at the first visit regardless of the final diagnosis. The consideration of age-dependent changes of these markers is helpful for diagnosing latent disorders based on various primary symptoms.
en-copyright=
kn-copyright=

en-aut-name=OmuraDaisuke
en-aut-sei=Omura
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SatoAsuka
en-aut-sei=Sato
en-aut-mei=Asuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OkaKosuke
en-aut-sei=Oka
en-aut-mei=Kosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HanayamaYoshihisa
en-aut-sei=Hanayama
en-aut-mei=Yoshihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OgawaHiroko
en-aut-sei=Ogawa
en-aut-mei=Hiroko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ObikaMikako
en-aut-sei=Obika
en-aut-mei=Mikako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OtsukaFumio
en-aut-sei=Otsuka
en-aut-mei=Fumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=dizziness
kn-keyword=dizziness
en-keyword=fever
kn-keyword=fever
en-keyword=osmolality
kn-keyword=osmolality
en-keyword=serum sodium
kn-keyword=serum sodium
en-keyword=thyroid dysfunction
kn-keyword=thyroid dysfunction
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180927
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Brugada症候群における予後予測指標としてのピルジカイニド負荷試験の有用性
kn-title=Prognostic Significance of the Sodium Channel Blocker Test in Patients With Brugada Syndrome
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=UeokaAkira
en-aut-sei=Ueoka
en-aut-mei=Akira
kn-aut-name=上岡亮
kn-aut-sei=上岡
kn-aut-mei=亮
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=岡山大学大学院医歯薬学総合研究科
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=5
article-no=
start-page=447
end-page=456
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=201810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Clinical Characteristics of Febrile Outpatients : Possible Involvement of Thyroid Dysfunction in Febrile Tachycardia
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= We retrospectively analyzed the cases of 148 febrile patients whose body temperature (BT) was ≥ 37.5°C at our hospital. We categorized them into seven groups; those with bacterial and viral infection, nonspecific inflammation, neoplasm, connective tissue disease (CTD), drug-induced disease, and unidentified causes. Our analysis revealed that the patient’s BT at the 1st visit (BT-1st visit) and highest BT during the febrile period (BT-max) differed significantly among all categories except neoplasm. The greatest difference between BT-1st visit and BT-max was highest in the CTD group (1.5°C). Positive correlations of heart rate and C-reactive protein (CRP) level with BT-max and a negative correlation between serum sodium level with BT-max were uncovered. The serum thyroid-stimulating hormone (TSH) level and the ratio of TSH/free thyroxine were negatively correlated with BT-max, especially in the viral infection group, suggesting the existence of occult thyrotoxicosis in accord with a febrile condition, possibly leading to febrile tachycardia. A febrile gap between BT-1st visit and BT-max (except in the neoplasm group) was shown, in which BT-max was correlated with thyroid function. Clinicians should recognize the fluctuation of BT when diagnosing febrile patients, and tachycardia in such febrile patients may be, at least in part, associated with subclinical thyroid dysfunction.
en-copyright=
kn-copyright=

en-aut-name=OkaKosuke
en-aut-sei=Oka
en-aut-mei=Kosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HanayamaYoshihisa
en-aut-sei=Hanayama
en-aut-mei=Yoshihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SatoAsuka
en-aut-sei=Sato
en-aut-mei=Asuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OmuraDaisuke
en-aut-sei=Omura
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YasudaMiho
en-aut-sei=Yasuda
en-aut-mei=Miho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HasegawaKo
en-aut-sei=Hasegawa
en-aut-mei=Ko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=ObikaMikako
en-aut-sei=Obika
en-aut-mei=Mikako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=OtsukaFumio
en-aut-sei=Otsuka
en-aut-mei=Fumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=body temperature
kn-keyword=body temperature
en-keyword=C-reactive protein
kn-keyword=C-reactive protein
en-keyword=fever of unknown origin
kn-keyword=fever of unknown origin
en-keyword=tachycardia
kn-keyword=tachycardia
en-keyword=thyroid dysfunction
kn-keyword=thyroid dysfunction
END

start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=
article-no=
start-page=265
end-page=270
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150514
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Epoprostenol sodium for treatment of pulmonary arterial hypertension
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　The release of endogenous prostacyclin (PGI2) is depressed in patients with pulmonary arterial hypertension (PAH). PGI2 replacement therapy by epoprostenol infusion is one of the best treatments available for PAH. Here, we provide an overview of the current clinical data for epoprostenol. Epoprostenol treatment improves symptoms, exercise capacity, and hemodynamics, and is the only treatment that has been shown to reduce mortality in patients with idiopathic PAH (IPAH) in randomized clinical trials. We have reported that high-dose epoprostenol therapy (>40 ng/kg/min) also results in marked hemodynamic improvement in some patients with IPAH. High-dose epoprostenol has a pro-apoptotic effect on PAH-PASMCs via the IP receptor and upregulation of Fas ligand (FasL) in vitro. However, long-term intravenous administration of epoprostenol is sometimes associated with catheter-related infections and leads to considerable inconvenience for the patient. In the future, the development of new routes of administration or the development of powerful PGI2 analogs, IP-receptor agonists, and gene and cell-based therapy enhancing PGI2 production with new routes of administration is required.
en-copyright=
kn-copyright=

en-aut-name=SaitoYukihiro
en-aut-sei=Saito
en-aut-mei=Yukihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NakamuraKazufumi
en-aut-sei=Nakamura
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AkagiSatoshi
en-aut-sei=Akagi
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SarashinaToshihiro
en-aut-sei=Sarashina
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=EjiriKentaro
en-aut-sei=Ejiri
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MiuraAya
en-aut-sei=Miura
en-aut-mei=Aya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OgawaAiko
en-aut-sei=Ogawa
en-aut-mei=Aiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MatsubaraHiromi
en-aut-sei=Matsubara
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=ItoHiroshi
en-aut-sei=Ito
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Division of Cardiology, National Hospital Organization Okayama Medical Center
kn-affil=

affil-num=7
en-affil=Division of Cardiology, National Hospital Organization Okayama Medical Center
kn-affil=

affil-num=8
en-affil=Division of Cardiology, National Hospital Organization Okayama Medical Center
kn-affil=

affil-num=9
en-affil=Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=apoptosis
kn-keyword=apoptosis
en-keyword=prostacyclin
kn-keyword=prostacyclin
en-keyword=pulmonary arterial hypertension
kn-keyword=pulmonary arterial hypertension
END

start-ver=1.4
cd-journal=joma
no-vol=129
cd-vols=
no-issue=1
article-no=
start-page=9
end-page=15
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170403
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Does hydrogen-rich water really work?
kn-title=水素水は怪しい水でしょうか？
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=NakaoAtsunori
en-aut-sei=Nakao
en-aut-mei=Atsunori
kn-aut-name=中尾篤典
kn-aut-sei=中尾
kn-aut-mei=篤典
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=岡山大学大学院医歯薬学総合研究科　救急医学
en-keyword=水素水
kn-keyword=水素水
en-keyword=抗酸化作用
kn-keyword=抗酸化作用
en-keyword=抗炎症作用
kn-keyword=抗炎症作用
en-keyword=臨床応用
kn-keyword=臨床応用
en-keyword=疑似科学
kn-keyword=疑似科学
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=1
article-no=
start-page=85
end-page=89
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=201702
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Syndrome of Inappropriate Antidiuretic Hormone Secretion Following Liver Transplantation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Syndrome of inappropriate antidiuretic hormone secretion (SIADH) is an extremely rare cause of hyponatremia post-liver transplantation. A 15-year-old Japanese girl with recurrent cholangitis after Kasai surgery for biliary atresia underwent successful living donor liver transplantation. Peritonitis due to gastrointestinal perforation occurred. Hyponatremia gradually developed but improved after hypertonic sodium treatment. One month later, severe hyponatremia rapidly recurred. We considered the hyponatremia’s cause as SIADH. We suspected that tacrolimus was the disease’s cause, so we used cyclosporine instead, plus hypertonic sodium plus water intake restriction, which improved the hyponatremia. Symptomatic hyponatremia manifested by SIADH is a rare, serious complication post-liver transplantation.
en-copyright=
kn-copyright=

en-aut-name=TakagiKosei
en-aut-sei=Takagi
en-aut-mei=Kosei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YagiTakahito
en-aut-sei=Yagi
en-aut-mei=Takahito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ShinouraSusumu
en-aut-sei=Shinoura
en-aut-mei=Susumu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=UmedaYuzo
en-aut-sei=Umeda
en-aut-mei=Yuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshidaRyuichi
en-aut-sei=Yoshida
en-aut-mei=Ryuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NobuokaDaisuke
en-aut-sei=Nobuoka
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=WatanabeNobuyuki
en-aut-sei=Watanabe
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KuiseTakashi
en-aut-sei=Kuise
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=FujiTomokazu
en-aut-sei=Fuji
en-aut-mei=Tomokazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ArakiHiroyuki
en-aut-sei=Araki
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=4
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=6
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=8
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=9
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=10
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=11
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=syndrome of inappropriate antidiuretic hormone secretion
kn-keyword=syndrome of inappropriate antidiuretic hormone secretion
en-keyword=SIADH
kn-keyword=SIADH
en-keyword=hyponatremia
kn-keyword=hyponatremia
en-keyword=liver transplantation
kn-keyword=liver transplantation
en-keyword=tacrolimus
kn-keyword=tacrolimus
END

start-ver=1.4
cd-journal=joma
no-vol=4
cd-vols=
no-issue=34
article-no=
start-page=13183
end-page=13193 
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160725
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Combination of solid state NMR and DFT calculation to elucidate the state of sodium in hard carbon electrodes 
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We examined the state of sodium electrochemically inserted in HC prepared at 700–2000 °C using solid state Na magic angle spinning (MAS) NMR and multiple quantum (MQ) MAS NMR. The 23Na MAS NMR spectra of Na-inserted HC samples showed signals only in the range between +30 and −60 ppm. Each observed spectrum was ascribed to combinations of Na+ ions from the electrolyte, reversible ionic Na components, irreversible Na components assigned to solid electrolyte interphase (SEI) or non-extractable sodium ions in HC, and decomposed Na compounds such as Na2CO3. No quasi-metallic sodium component was observed to be dissimilar to the case of Li inserted in HC. MQMAS NMR implies that heat treatment of HC higher than 1600 °C decreases defect sites in the carbon structure. To elucidate the difference in cluster formation between Na and Li in HC, the condensation mechanism and stability of Na and Li atoms on a carbon layer were also studied using DFT calculation. Na3 triangle clusters standing perpendicular to the carbon surface were obtained as a stable structure of Na, whereas Li2 linear and Li4 square clusters, all with Li atoms being attached directly to the surface, were estimated by optimization. Models of Na and Li storage in HC, based on the calculated cluster structures were proposed, which elucidate why the adequate heat treatment temperature of HC for high-capacity sodium storage is higher than the temperature for lithium storage.
en-copyright=
kn-copyright=

en-aut-name=MoritaRyohei
en-aut-sei=Morita
en-aut-mei=Ryohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=GotohKazuma
en-aut-sei=Gotoh
en-aut-mei=Kazuma
kn-aut-name=後藤和馬
kn-aut-sei=後藤
kn-aut-mei=和馬
aut-affil-num=2
ORCID=

en-aut-name=FukunishiMika
en-aut-sei=Fukunishi
en-aut-mei=Mika
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KubotaKei
en-aut-sei=Kubota
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KomabaShinichi
en-aut-sei=Komaba
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NishimuraNaoto
en-aut-sei=Nishimura
en-aut-mei=Naoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YumuraTakashi
en-aut-sei=Yumura
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=DeguchiKenzo
en-aut-sei=Deguchi
en-aut-mei=Kenzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OhkiShinobu
en-aut-sei=Ohki
en-aut-mei=Shinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=ShimizueTadashi
en-aut-sei=Shimizue
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=IshidaHiroyuki
en-aut-sei=Ishida
en-aut-mei=Hiroyuki
kn-aut-name=石田祐之
kn-aut-sei=石田
kn-aut-mei=祐之
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科

affil-num=3
en-affil=Elements Strategy Initiative for Catalysts and Batteries (ESICB), Kyoto University
kn-affil=

affil-num=4
en-affil=Elements Strategy Initiative for Catalysts and Batteries (ESICB), Kyoto University
kn-affil=

affil-num=5
en-affil=Elements Strategy Initiative for Catalysts and Batteries (ESICB), Kyoto University
kn-affil=

affil-num=6
en-affil=Department of Chemistry and Materials Technology, Kyoto Institute of Technology
kn-affil=

affil-num=7
en-affil=Department of Chemistry and Materials Technology, Kyoto Institute of Technology
kn-affil=

affil-num=8
en-affil=National Institute for Materials Science
kn-affil=

affil-num=9
en-affil=National Institute for Materials Science
kn-affil=

affil-num=10
en-affil=National Institute for Materials Science
kn-affil=

affil-num=11
en-affil=Graduate School of Natural Science & Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科
END

start-ver=1.4
cd-journal=joma
no-vol=1440
cd-vols=
no-issue=
article-no=
start-page=145
end-page=149
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Direct determination of lignin peroxidase released from Phanerochaete chrysosporium by in-capillary enzyme assay using micellar electrokinetic chromatography
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Here we describe the application of an in-capillary enzyme assay using micellar electrokinetic chromatography (MEKC) in the determination of enzyme activity in a crude culture medium containing lignin peroxidase released from Phanerochaete chrysosporium (P. chrysosporium). The method consists of a plug–plug reaction between lignin peroxidase and its substrate, veratryl alcohol, the separation of the product, veratraldehyde, from the other components including the enzyme and the culture medium, and the determination of the enzyme activity from the peak area of veratraldehyde produced by the plug–plug reaction. This method is more sensitive than conventional spectrophotometry since the background originates from the enzyme and the culture medium can be removed via MEKC separation. Veratraldehyde was separated at −10 kV in a background electrolyte containing 50 mM tartrate buffer (pH 2.5) and 50 mM sodium dodecyl sulfate (SDS) after a plug–plug reaction in the capillary for 5 min. The calibration curve of veratraldehyde was linear up to 4 pmol (500 μM) with a limit to quantification of 0.026 pmol (3.2 μM) (SN = 10). The activity of lignin peroxidase was directly measured from the peak area of veratraldehyde. The activity of lignin peroxidase released from P. chrysosporium into the medium for 7 days was successfully determined to be 3.40 UL−1.
en-copyright=
kn-copyright=

en-aut-name=HaradaAiri
en-aut-sei=Harada
en-aut-mei=Airi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SasakiKeiko
en-aut-sei=Sasaki
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Department of Earth Resources Engineering, Graduate School of Engineering, Kyushu University

affil-num=3
en-affil=
kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
en-keyword=Lignin Peroxidase
kn-keyword=Lignin Peroxidase
en-keyword=Phanerochaete chrysosporium
kn-keyword=Phanerochaete chrysosporium
en-keyword=Micellar electrokinetic chromatography
kn-keyword=Micellar electrokinetic chromatography
en-keyword=Capillary electrophoresis
kn-keyword=Capillary electrophoresis
en-keyword=Enzyme assay
kn-keyword=Enzyme assay
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=4
article-no=
start-page=213
end-page=218
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=201508
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Prevalence, Risk Factors, and Short-term Consequences of Traumatic Brain Injury-associated Hyponatremia
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hyponatremia, a common electrolyte disorder associated with traumatic brain injuries (TBIs), has high mortality and morbidity rates. The aim of this study was to identify the risk factors for hyponatremia associated with TBI. We retrospectively analyzed the cases of TBI patients who were admitted to the emergency intensive care unit at Okayama University Hospital between October 2011 and September 2014. A total of 82 TBI patients were enrolled. The incidences of hyponatremia (serum sodium level of＜135mEq/L) and severe hyponatremia (serum sodium level of＜130mEq/L) within the first 14 days after admission were 51ｵ (n＝42) and 20ｵ (n＝16), respectively. After admission, hyponatremia took a median period of 7 days to develop and lasted for a median of 3 days. Multivariate analysis demonstrated that higher fluid intake from days 1 to 3 and the presence of cranial fractures were risk factors for hyponatremia. The 58 patients with hyponatremia experienced fewer ventilator-free days, longer intensive care unit stays, and less favorable outcomes compared to the 24 patients without hyponatremia;however, these differences were not significant. Further studies are needed to determine the optimal management strategy for TBI-associated hyponatremia in the intensive care unit setting.
en-copyright=
kn-copyright=

en-aut-name=YumotoTetsuya
en-aut-sei=Yumoto
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SatoKeiji
en-aut-sei=Sato
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UgawaToyomu
en-aut-sei=Ugawa
en-aut-mei=Toyomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=IchibaShingo
en-aut-sei=Ichiba
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=UjikeYoshihito
en-aut-sei=Ujike
en-aut-mei=Yoshihito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=2
en-affil=
kn-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=3
en-affil=
kn-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=4
en-affil=
kn-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=5
en-affil=
kn-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
en-keyword=traumatic brain injury
kn-keyword=traumatic brain injury
en-keyword=hyponatremia
kn-keyword=hyponatremia
en-keyword=cranial fracture
kn-keyword=cranial fracture
en-keyword=fluid intake
kn-keyword=fluid intake
END

start-ver=1.4
cd-journal=joma
no-vol=128
cd-vols=
no-issue=3
article-no=
start-page=419
end-page=424
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080301
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of postprandial hyperglycemia and hyperinsulinemia on vascular responsiveness
kn-title=食後高血糖が血管反応性に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Recent clinical studies demonstrated that transient postprandial hyperglycemia and hyperinsulinemia may contribute to the development of hypertension. Therefore, we investigated influence of acute hyperglycemia and/or hyperinsulinemia induced by glucose or insulin infusion on neuronal and humoral control of vascular tone in rats. Euglycemic male Wistar rats were pithed under anesthesia and arterial blood pressure was measured. Changes in vascular responses to spinal cord stimulation (SCS) and intravenous bolus injections of noradrenaline, angiotensin II, calcitonin generelated peptide (CGRP), acetylcholine and sodium nitroprusside (SNP) were studied by infusing various concentration of glucose or insulin. Continuous glucose infusion, which increased both blood glucose and serum insulin levels, significantly augmented adrenergic nerve-mediated pressor responses to SCS without affecting injection of pressor responses to noradrenaline or angiotensin II. In pithed rats with artificially increased blood pressure and blockade of autonomic outflow, glucose infusion attenuated CGRPergic nerve-depressor responses to SCS without affecting depressor responses to injection of CGRP, acetylcholine or SNP. In pithed rats treated with octreotide, which increased blood glucose without increasing serum insulin levels, glucose infusion caused only significant augmentation of adrenergic nervemediated pressor responses. Combined infusion of insulin and glucose, which resulted in increased serum insulin levels with euglycemic, significantly augmented adrenergic nerve-mediated pressor responses and attenuated CGRPergic nerve-mediated depressor responses. The present results suggest that acute hyperglycemia and hyperinsulinemia increases adrenergic nerve-mediated vasoconstriction, which is partly associated with the blunted CGRPergic nerve function, and that plasma insulin concentration associated with hyperglycemia may be responsible for alteration of neuronal vascular regulation.
en-copyright=
kn-copyright=

en-aut-name=ZamamiYoshito
en-aut-sei=Zamami
en-aut-mei=Yoshito
kn-aut-name=座間味義人
kn-aut-sei=座間味
kn-aut-mei=義人
aut-affil-num=1
ORCID=

en-aut-name=TakatoriShingo
en-aut-sei=Takatori
en-aut-mei=Shingo
kn-aut-name=高取真吾
kn-aut-sei=高取
kn-aut-mei=真吾
aut-affil-num=2
ORCID=

en-aut-name=IwataniYukiko
en-aut-sei=Iwatani
en-aut-mei=Yukiko
kn-aut-name=岩谷有希子
kn-aut-sei=岩谷
kn-aut-mei=有希子
aut-affil-num=3
ORCID=

en-aut-name=YamawakiKosuke
en-aut-sei=Yamawaki
en-aut-mei=Kosuke
kn-aut-name=山脇康佑
kn-aut-sei=山脇
kn-aut-mei=康佑
aut-affil-num=4
ORCID=

en-aut-name=MiyashitaSatoko
en-aut-sei=Miyashita
en-aut-mei=Satoko
kn-aut-name=宮下智子
kn-aut-sei=宮下
kn-aut-mei=智子
aut-affil-num=5
ORCID=

en-aut-name=YabumaeNana
en-aut-sei=Yabumae
en-aut-mei=Nana
kn-aut-name=藪前奈々
kn-aut-sei=藪前
kn-aut-mei=奈々
aut-affil-num=6
ORCID=

en-aut-name=TakayamaFusako
en-aut-sei=Takayama
en-aut-mei=Fusako
kn-aut-name=高山房子
kn-aut-sei=高山
kn-aut-mei=房子
aut-affil-num=7
ORCID=

en-aut-name=MioMitsunobu
en-aut-sei=Mio
en-aut-mei=Mitsunobu
kn-aut-name=見尾光庸
kn-aut-sei=見尾
kn-aut-mei=光庸
aut-affil-num=8
ORCID=

en-aut-name=KawasakiHiromu
en-aut-sei=Kawasaki
en-aut-mei=Hiromu
kn-aut-name=川﨑博己
kn-aut-sei=川﨑
kn-aut-mei=博己
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=2
en-affil=
kn-affil=日本新薬(株)創薬研究所

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=5
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=6
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=7
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=8
en-affil=
kn-affil=就実大学薬学部薬効解析学分野

affil-num=9
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野
en-keyword=hyperglycemia
kn-keyword=hyperglycemia
en-keyword=hyperinsulinemia
kn-keyword=hyperinsulinemia
en-keyword=Calcitonin gene-related peptide nerve
kn-keyword=Calcitonin gene-related peptide nerve
END

start-ver=1.4
cd-journal=joma
no-vol=130
cd-vols=
no-issue=6
article-no=
start-page=833
end-page=840
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100601
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Ameliorative effect of propolis on insulin resistance in Otsuka Long-Evans Tokushima Fatty (OLETF) rats
kn-title=Propolis 長期投与による Otsuka Long-Evans Tokushima Fatty (OLETF) ラットの インスリン抵抗性改善作用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Propolis is known to have abundant bioactive constituents and a variety of biological activities. To investigate the effect of Brazilian propolis on insulin resistance, 10-week-old Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a non-insulin-dependent type 2 diabetic model, were treated for 4 weeks with propolis (100 and 300 mg/kg, p.o.) or vehicle (control). Propolis treatment significantly decreased the plasma levels of insulin and insulin resistance index (Homeostasis Model Assessment-Insulin Resistance; HOM-IR), without affecting blood glucose levels and tended to lower systolic blood pressure compared with the control. In isolated and perfused mesenteric vascular beds of OLETF rats, propolis treatment resulted in significant reduction of sympathetic nerve-mediated vasoconstrictor response to periarterial nerve stimulation (PNS) and tended to increase calcitonin gene-related peptide (CGRP) nerve-mediated vasodilator response to PNS compared with in vehicle-treated OLETF rats. However, propolis treatment did not significantly affect the vasoconstrictor and vasodilator response to noradrenaline, CGRP, acetylcholine, and sodium nitroprusside. These results suggest that propolis could be an effective and functional food to prevent development of insulin resistance.
en-copyright=
kn-copyright=

en-aut-name=ZamamiYoshito
en-aut-sei=Zamami
en-aut-mei=Yoshito
kn-aut-name=座間味義人
kn-aut-sei=座間味
kn-aut-mei=義人
aut-affil-num=1
ORCID=

en-aut-name=FujiwaraHiroki
en-aut-sei=Fujiwara
en-aut-mei=Hiroki
kn-aut-name=藤原弘喜
kn-aut-sei=藤原
kn-aut-mei=弘喜
aut-affil-num=2
ORCID=

en-aut-name=HosodaMiho
en-aut-sei=Hosoda
en-aut-mei=Miho
kn-aut-name=細田美穂
kn-aut-sei=細田
kn-aut-mei=美穂
aut-affil-num=3
ORCID=

en-aut-name=HinoHayato
en-aut-sei=Hino
en-aut-mei=Hayato
kn-aut-name=日野隼人
kn-aut-sei=日野
kn-aut-mei=隼人
aut-affil-num=4
ORCID=

en-aut-name=HiraiKazuhiro
en-aut-sei=Hirai
en-aut-mei=Kazuhiro
kn-aut-name=平井和浩
kn-aut-sei=平井
kn-aut-mei=和浩
aut-affil-num=5
ORCID=

en-aut-name=OkamotoKazuaki
en-aut-sei=Okamoto
en-aut-mei=Kazuaki
kn-aut-name=岡本和明
kn-aut-sei=岡本
kn-aut-mei=和明
aut-affil-num=6
ORCID=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=金鑫
kn-aut-sei=金
kn-aut-mei=鑫
aut-affil-num=7
ORCID=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=高取真吾
kn-aut-sei=高取
kn-aut-mei=真吾
aut-affil-num=8
ORCID=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=高木志真
kn-aut-sei=高木
kn-aut-mei=志真
aut-affil-num=9
ORCID=

en-aut-name=KawasakiHiromu
en-aut-sei=Kawasaki
en-aut-mei=Hiromu
kn-aut-name=川﨑博己
kn-aut-sei=川﨑
kn-aut-mei=博己
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=2
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=5
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=6
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=7
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野

affil-num=8
en-affil=
kn-affil=日本新薬株式会社創薬研究所

affil-num=9
en-affil=
kn-affil=山田養蜂場本社研究開発部

affil-num=10
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野
en-keyword=propolis
kn-keyword=propolis
en-keyword=insulin resistance
kn-keyword=insulin resistance
en-keyword=Otsuka Long-Evans Tokushima Fatty rat
kn-keyword=Otsuka Long-Evans Tokushima Fatty rat
en-keyword=periarterial nerve function
kn-keyword=periarterial nerve function
en-keyword=mesenteric vascular bed
kn-keyword=mesenteric vascular bed
END

start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=
article-no=
start-page=33
end-page=36
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=201504
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Environmental Disinfection by Hypochlorous Acid Solution
kn-title=次亜塩素酸水溶液による環境消毒について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Weak Acid Hypochlorous Solution (WAHS) has been used for disinfection of foods (meats, vegetables etc.), and for environmental disinfection in the Retirement homes, Hospitals and Laboratory Animal facilities. We will introduce here some of environmental disinfection tests. 1) To study whether WAHS is available or not for blood
blot inoculated by Acinetobacter baumannii on the plate comparing with Sodium Hypochlorite. 2) Comparison of Ethanol and WAHS on the floor and handrail. 3) To study efficacy of shallowly dipping by WAHS on wagon caster inoculated by Staphylococcus aureus.
The results are: 1) It was observed that WAHS had an efficacy equal to Hypochlorite with lower concentration in the blood test, but in case that the adhesion amount of blood was larger, much higher concentration or adding
physical removal was needed. 2) Ethanol and WAHS had Equivalent efficacy on the test of floor and handrail. 3) It was suggested that shallowly dipping by WAHS was available for disinfection of wagon caster. We hope to proceed to confirm how to use WAHS for environmental disinfection.
en-copyright=
kn-copyright=

en-aut-name=YamashitaKoji
en-aut-sei=Yamashita
en-aut-mei=Koji
kn-aut-name=山下光治
kn-aut-sei=山下
kn-aut-mei=光治
aut-affil-num=1
ORCID=

en-aut-name=HamamotoYuji
en-aut-sei=Hamamoto
en-aut-mei=Yuji
kn-aut-name=濱本裕司
kn-aut-sei=濱本
kn-aut-mei=裕司
aut-affil-num=2
ORCID=

en-aut-name=YasudaYuto
en-aut-sei=Yasuda
en-aut-mei=Yuto
kn-aut-name=安田悠人
kn-aut-sei=安田
kn-aut-mei=悠人
aut-affil-num=3
ORCID=

en-aut-name=OnoTomoko
en-aut-sei=Ono
en-aut-mei=Tomoko
kn-aut-name=小野朋子
kn-aut-sei=小野
kn-aut-mei=朋子
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=株式会社 エイチ・エス・ピー

affil-num=2
en-affil=
kn-affil=株式会社 エイチ・エス・ピー

affil-num=3
en-affil=
kn-affil=株式会社 エイチ・エス・ピー

affil-num=4
en-affil=
kn-affil=株式会社 エイチ・エス・ピー
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=SGLT2阻害薬ダパグリフロジン長期投与によるdb/dbマウスの糖代謝および糖尿病性腎症の改善効果
kn-title=Long-Term Treatment with the Sodium Glucose Cotransporter 2 Inhibitor, Dapagliflozin, Ameliorates Glucose Homeostasis and Diabetic Nephropathy in db/db Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=TeramiNaoto
en-aut-sei=Terami
en-aut-mei=Naoto
kn-aut-name=寺見直人
kn-aut-sei=寺見
kn-aut-mei=直人
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院
END

start-ver=1.4
cd-journal=joma
no-vol=92
cd-vols=
no-issue=1
article-no=
start-page=46
end-page=53
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=201401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Reducing Hemorrhagic Complication by Dabigatran Via Neurovascular Protection After Recanalization With Tissue Plasminogen Activator in Ischemic Stroke of Rat
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study assesses the risks and benefits of tissue plasminogen activator (tPA) treatment under oral anticoagulation with dabigatran compared with warfarin or vehicle control in transient middle cerebral artery occlusion (tMCAO). After pretreatment with warfarin (0.2 mg/kg/day), dabigatran (20 mg/kg/day), or vehicle (0.5% carboxymethyl cellulose sodium salt) for 7 days, tMCAO was induced for 120 min, followed by reperfusion and tPA (10 mg/kg/10 ml). Clinical parameters, including cerebral infarction volume, hemorrhagic volume, and blood coagulation, were examined. At 24 hr after reperfusion, markers for the neurovascular unit at the peri-ischemic lesion were immunohistochemically examined in brain sections, and MMP-9 activity was measured by zymography. Paraparesis and intracerebral hemorrhage volume were significantly improved in the dabigatran-pretreated group compared with the warfarin-pretreated group. A marked dissociation between astrocyte foot processes and the basal lamina or pericyte was observed in the warfarin-pretreated group, which was greatly improved in the dabigatran-pretreated group. Furthermore, a remarkable activation of MMP-9 in the ipsilateral warfarin-pretreated rat brain was greatly reduced in dabigatran-pretreated rats. The present study reveals that the mechanism of intracerebral hemorrhage with warfarin-pretreatment plus tPA in ischemic stroke rats is the dissociation of the neurovascular unit, including the pericyte. Neurovascular protection by dabigatran, which was first shown in this study, could partially explain the reduction in hemorrhagic complication by dabigatran reported from clinical study.
en-copyright=
kn-copyright=

en-aut-name=KonoSyoichiro
en-aut-sei=Kono
en-aut-mei=Syoichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=DeguchiKentaro
en-aut-sei=Deguchi
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OmoteYoshio
en-aut-sei=Omote
en-aut-mei=Yoshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YunokiTaijun
en-aut-sei=Yunoki
en-aut-mei=Taijun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YamashitaToru
en-aut-sei=Yamashita
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KurataTomoko
en-aut-sei=Kurata
en-aut-mei=Tomoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=IkedaYoshio
en-aut-sei=Ikeda
en-aut-mei=Yoshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=AbeKoji
en-aut-sei=Abe
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol

affil-num=2
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol

affil-num=4
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol

affil-num=6
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol

affil-num=7
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol

affil-num=8
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol
en-keyword=dabigatran
kn-keyword=dabigatran
en-keyword=hemorrhagic complication
kn-keyword=hemorrhagic complication
en-keyword=neurovascular unit
kn-keyword=neurovascular unit
en-keyword=pericyte
kn-keyword=pericyte
en-keyword=thrombolysis
kn-keyword=thrombolysis
en-keyword=tPA
kn-keyword=tPA
END

start-ver=1.4
cd-journal=joma
no-vol=52
cd-vols=
no-issue=5
article-no=
start-page=1010
end-page=1017
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=201105
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Therapy for hyperthermia-induced seizures in Scn1a mutant rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Purpose: Mutations in the SCN1A gene, which encodes the alpha 1 subunit of voltage-gated sodium channels, cause generalized epilepsy with febrile seizures plus (GEFS+) and severe myoclonic epilepsy of infancy (SMEI). N1417H-Scn1a mutant rats are considered to be an animal model of human FS+ or GEFS+. To assess the pharmacologic validity of this model, we compared the efficacies of eight different antiepileptic drugs (AEDs) for the treatment of hyperthermia-induced seizures using N1417H-Scn1a mutant rats. 

Methods: AEDs used in this study included valproate, carbamazepine (CBZ), phenobarbital, gabapentin, acetazolamide, diazepam (DZP), topiramate, and potassium bromide (KBr). The effects of these AEDs were evaluated using the hot water model, which is a model of experimental FS. Five-week-old rats were pretreated with each AED and immersed in water at 45 degrees C to induce hyperthermia-induced seizures. The seizure manifestations and video-electroencephalographic recordings were evaluated. Furthermore, the effects of each AED on motor coordination and balance were assessed using the balance-beam test. 

Key Findings: KBr significantly reduced seizure durations, and its anticonvulsant effects were comparable to those of DZP. On the other hand, CBZ decreased the seizure threshold. In addition, DZP and not KBr showed significant impairment in motor coordination and balance. 

Significance: DZP and KBr showed potent inhibitory effects against hyperthermia-induced seizures in the Scn1a mutant rats, whereas CBZ exhibited adverse effects. These responses to hyperthermia-induced seizures were similar to those in patients with GEFS+ and SMEI. N1417H-Scn1a mutant rats may, therefore, be useful for testing the efficacy of new AEDs against FS in GEFS+ and SMEI patients.
en-copyright=
kn-copyright=

en-aut-name=HayashiKeiichiro
en-aut-sei=Hayashi
en-aut-mei=Keiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UeshimaSatoshi
en-aut-sei=Ueshima
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OuchidaMamoru
en-aut-sei=Ouchida
en-aut-mei=Mamoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MashimoTomoji
en-aut-sei=Mashimo
en-aut-mei=Tomoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NishikiTeiichi
en-aut-sei=Nishiki
en-aut-mei=Teiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SendoToshiaki
en-aut-sei=Sendo
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SerikawaTadao
en-aut-sei=Serikawa
en-aut-mei=Tadao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MatsuiHideki
en-aut-sei=Matsui
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OhmoriIori
en-aut-sei=Ohmori
en-aut-mei=Iori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol

affil-num=2
en-affil=
kn-affil=Okayama Univ Hosp, Dept Pharm

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Genet

affil-num=4
en-affil=
kn-affil=Kyoto Univ, Grad Sch Med, Inst Lab Anim

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol

affil-num=6
en-affil=
kn-affil=Okayama Univ Hosp, Dept Pharm

affil-num=7
en-affil=
kn-affil=Kyoto Univ, Grad Sch Med, Inst Lab Anim

affil-num=8
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol

affil-num=9
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Physiol
en-keyword=Febrile seizure
kn-keyword=Febrile seizure
en-keyword=Animal models
kn-keyword=Animal models
en-keyword=Scn1a gene
kn-keyword=Scn1a gene
en-keyword=Generalized epilepsy with febrile seizures plus
kn-keyword=Generalized epilepsy with febrile seizures plus
en-keyword=Severe myoclonic epilepsy of infancy
kn-keyword=Severe myoclonic epilepsy of infancy
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ラドン吸入が酸化ストレス関連疾患モデルマウスに及ぼす抗酸化・抗炎症作用に関する研究 第１編 ラドン吸入によるストレプトゾトシン誘導マウス１型糖尿病の抑制 第２編 ラドン吸入によるデキストラン硫酸ナトリウム誘導マウス大腸炎の抑制
kn-title=Study on anti-oxidative and anti-inflammatory effects of radon inhalation on mouse models of oxidative stress-related disease 第１編　Suppression of streptozotocin-induced type-1 diabetes in mice by radon inhalation 第２編　Suppression of dextran sulfate sodium-induced colitis in mice by radon inhalation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=NishiyamaYuichi
en-aut-sei=Nishiyama
en-aut-mei=Yuichi
kn-aut-name=西山祐一
kn-aut-sei=西山
kn-aut-mei=祐一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END

start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=
article-no=
start-page=7
end-page=7
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=201404
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Abnormality of water homeostasis of Muscular dystrophic chicken
kn-title=筋ジストロフィーニワトリの水分代謝異常
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The muscular dystrophy chicken has been studying as model animal of muscular dystrophy for more
than 50 years. Recently, the mutation of WW domain containing E3 ubiquitin protein ligase 1 (WWP1)
gene has been identified as a responsible for muscular dystrophy chicken. We observed that muscular
dystrophy chicken not only showed the degeneration of skeletal muscles but also produced watery feces.
Therefore, we examined the possibility of abnormalities in water metabolism of muscular dystrophy
chicken. We first analyzed plasma osmolality and gene expression of aquaporin 2 (AQP2), AQP3 and
alpha subunit of the amiloride-sensitive epithelial sodium channel (αENaC) in muscular dystrophy
chicken and White Leghorn chicken under normal physiological conditions at five-week old.
Subsequently, we analyzed these same parameters after one-day water-deprivation. The main findings of
our study are that: I) the plasma osmolality was significantly higher in muscular dystrophic chicken than
in White Leghorn; II) kidney αENaC mRNA expression was significantly lower in muscular dystrophic
chicken than in White Leghorn; III) AQP2 and AQP3 mRNA expressions in muscular dystrophic chicken
were similar in White Leghorn. We suggest that the mutation of WWP1 may cause the abnormality of
sodium absorption, and thus muscular dystrophic chicken become hypernatremic.
en-copyright=
kn-copyright=

en-aut-name=SaitoNoboru
en-aut-sei=Saito
en-aut-mei=Noboru
kn-aut-name=齋藤昇
kn-aut-sei=齋藤
kn-aut-mei=昇
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院環境生命科学研究科
END

start-ver=1.4
cd-journal=joma
no-vol=235
cd-vols=
no-issue=
article-no=
start-page=59
end-page=69
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130403
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=EFFECT OF LIDOCAINE ON DYNAMIC CHANGES IN CORTICAL REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE FLUORESCENCE DURING TRANSIENT FOCAL CEREBRAL ISCHEMIA IN RATS
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Rats were subjected to 90 min of focal ischemia by occluding the left middle cerebral and both common carotid arteries. The dynamic changes in the formation of brain ischemic areas were analyzed by measuring the direct current (DC) potential and reduced nicotinamide adenine dinucleotide (NADH) fluorescence with ultraviolet irradiation. In the lidocaine group (n = 10), 30 min before ischemia, an intravenous bolus (1.5 mg/kg) of lidocaine was administered, followed by a continuous infusion (2 mg/kg/h) for 150 min. In the control group (n = 10), an equivalent amount of saline was administered. Following the initiation of ischemia, an area of high-intensity NADH fluorescence rapidly developed in the middle cerebral artery territory in both groups and the DC potential in this area showed ischemic depolarization. An increase in NADH fluorescence closely correlated with the DC depolarization. The blood flow in the marginal zone of both groups showed a similar decrease. Five minutes after the onset of ischemia, the area of high-intensity NADH fluorescence was significantly smaller in the lidocaine group (67% of the control; P = 0.01). This was likely due to the suppression of ischemic depolarization by blockage of voltage-dependent sodium channels with lidocaine. Although lidocaine administration did not attenuate the number of pen-infarct depolarizations during ischemia, the high-intensity area and infarct volume were significantly smaller in the lidocaine group both at the end of ischemia (78% of the control; P = 0.046) and 24 h later (P = 0.02). A logistic regression analysis demonstrated a relationship between the duration of ischemic depolarization and histologic damage and revealed that lidocaine administration did not attenuate neuronal damage when the duration of depolarization was identical. These findings indicate that the mechanism by which lidocaine decreases infarct volume is primarily through a reduction of the brain area undergoing NADH fluorescence increases which closely correlates with depolarization.
en-copyright=
kn-copyright=

en-aut-name=NaitoH.
en-aut-sei=Naito
en-aut-mei=H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakedaY.
en-aut-sei=Takeda
en-aut-mei=Y.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=DanuraT.
en-aut-sei=Danura
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KassI. S.
en-aut-sei=Kass
en-aut-mei=I. S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MoritaK.
en-aut-sei=Morita
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Sch Med, Dept Anesthesiol

affil-num=2
en-affil=
kn-affil=Okayama Univ, Sch Med, Dept Anesthesiol

affil-num=3
en-affil=
kn-affil=Okayama Univ, Sch Med, Dept Anesthesiol

affil-num=4
en-affil=
kn-affil=Suny Downstate Med Ctr, Dept Anesthesiol

affil-num=5
en-affil=
kn-affil=Okayama Univ, Sch Med, Dept Anesthesiol
en-keyword=hypoxia
kn-keyword=hypoxia
en-keyword=infarction
kn-keyword=infarction
en-keyword=lidocaine
kn-keyword=lidocaine
en-keyword=NADH fluorescence
kn-keyword=NADH fluorescence
en-keyword=DC potential
kn-keyword=DC potential
en-keyword=depolarization
kn-keyword=depolarization
END

start-ver=1.4
cd-journal=joma
no-vol=47
cd-vols=
no-issue=3
article-no=
start-page=137
end-page=145
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201305
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Inhibition of arginase ameliorates experimental ulcerative colitis in mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Nitric oxide (NO) is produced from the conversion of L-arginine by NO synthase (NOS) and regulates a variety of processes in the gastrointestinal tract. Considering the increased activity of arginase in colitis tissue, it is speculated that arginase could inhibit NO synthesis by competing for the same L-arginine substrate, resulting in the exacerbation of colitis. We examined the role of arginase and its relationship to NO metabolism in dextran sulfate sodium (DSS)-induced colitis. Experimental colitis was induced in mice by administration of 2.5% DSS in drinking water for 8 days. Treatment for arginase inhibition was done by once daily intraperitoneal injection of N-omega-hydroxy-norarginine (nor-NOHA). On day 8, we evaluated clinical parameters (body weight, disease activity index, and colon length), histological features, the activity and expression of arginase, L-arginine content, the expression of NO synthase (NOS), and the concentration of NO end-product (NOx: nitrite + nitrate). Administration of nor-NOHA improved the worsened clinical parameters and histological features in DSS-induced colitis. Treatment with nor-NOHA attenuated the increased activity of arginase, upregulation of arginase. at both mRNA and protein levels, and decreased the content of L-arginine in colonic tissue in the DSS-treated mice. Conversely, despite the decreased expression of NOS2 mRNA, the decreased concentration of NOx in colonic tissues was restored to almost normal levels. The consumption of L-arginine by arginase could lead to decreased production of NO from NOS, contributing to the pathogenesis of the colonic inflammation; thus, arginase inhibition might be effective for improving colitis.
en-copyright=
kn-copyright=

en-aut-name=AkazawaY.
en-aut-sei=Akazawa
en-aut-mei=Y.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KuboM.
en-aut-sei=Kubo
en-aut-mei=M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ZhangR.
en-aut-sei=Zhang
en-aut-mei=R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MatsumotoK.
en-aut-sei=Matsumoto
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YanF.
en-aut-sei=Yan
en-aut-mei=F.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SetiawanH.
en-aut-sei=Setiawan
en-aut-mei=H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakahashiH.
en-aut-sei=Takahashi
en-aut-mei=H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=FujikuraY.
en-aut-sei=Fujikura
en-aut-mei=Y.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OginoK.
en-aut-sei=Ogino
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth

affil-num=2
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth

affil-num=4
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth

affil-num=6
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth

affil-num=7
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth

affil-num=8
en-affil=
kn-affil=Oita Univ, Fac Med, Dept Mol Anat

affil-num=9
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
en-keyword=nitric oxide
kn-keyword=nitric oxide
en-keyword=N-omega-hydroxy-nor-L-arginine
kn-keyword=N-omega-hydroxy-nor-L-arginine
en-keyword=L-arginine
kn-keyword=L-arginine
en-keyword=NOx
kn-keyword=NOx
en-keyword=dextran sulfate sodium
kn-keyword=dextran sulfate sodium
END

start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=11
article-no=
start-page=2854
end-page=2859
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201305
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Determination of polyamines in Arabidopsis thaliana by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Herein, we report a novel method for the determination of polyamines in a sample extracted from Arabidopsis thaliana by capillary electrophoresis (CE) using salicylaldehyde-5-sulfonate (SAS) as a derivatizing reagent. An aldehyde group of SAS forms a Schiff base with amino groups of aliphatic polyamines, resulting in an anionic species with an absorption band in the ultraviolet region. The derivatization method was straightforward since the derivatives were formed by mixing a sample with the derivatizing reagent at a neutral pH. In addition, the negative charges induced by SAS led to a high resolution with a short analysis time. This method permitted the separation of five polyamines, which play important roles in plants. However, further improvement in sensitivity was needed for the determination of the polyamines in plant samples. Therefore, the CE method was coupled with solid-phase extraction (SPE) using an ion-pairing formation with sodium dodecyl benzene sulfonate. The SPE method improved the concentration limits of detection to sub-μM levels, which corresponded with a 10-fold enhancement. The calibration curves for cadaverine, putrescine, and spermidine were linear with concentrations that ranged from 1 to 20 μM and correlation coefficients (R2) were greater than 0.998. The proposed method was applied to the determination of spermidine in a plant sample, Arabidopsis thaliana.
en-copyright=
kn-copyright=

en-aut-name=InoueGenki
en-aut-sei=Inoue
en-aut-mei=Genki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakayanagiToshio
en-aut-sei=Takayanagi
en-aut-mei=Toshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KakehiJunichi
en-aut-sei=Kakehi
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MotoseHiroyasu
en-aut-sei=Motose
en-aut-mei=Hiroyasu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakahashiTaku
en-aut-sei=Takahashi
en-aut-mei=Taku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Chemistry, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Department of Chemistry, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Department of Life System, Institute of Technology and Science, The University of Tokushima

affil-num=4
en-affil=
kn-affil=Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University

affil-num=5
en-affil=
kn-affil=Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University

affil-num=6
en-affil=
kn-affil=Department of Biological Science, Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=1
article-no=
start-page=19
end-page=28
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1973
dt-pub=1973
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=THE ROLE OF CALCIUM IN SELECTIVE CATION UPTAKE BY PLANT ROOTS II. Effects of Temperature，Desorption Treatment and Sodium Salt on Rubidium Uptake*
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=KawasakiToshio
en-aut-sei=Kawasaki
en-aut-mei=Toshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HoriShiro
en-aut-sei=Hori
en-aut-mei=Shiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=29
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Day Length, Supplemental Lighting Strength, Shading Period and Minimum Night Temperature on Occurrence of Abnormal Inflorescence in Gypsophila paniculata ‘Altair’
kn-title=日長，補光強度，遮光時期および最低夜温がシュッコンカスミソウ ‘アルタイル’の形態異常花序発生に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=シュッコンカスミソウ‘アルタイル’の形態異常花序の発生には環境要因が関与していると考えられたので，日長，補光強度，遮光時期および最低夜温が形態異常花序発生に及ぼす影響を調査した．形態異常程度は４種類のパターン (０：正常，１：茎が短いもの，２：２本の茎が癒着，3：ひどく湾曲し変形したもの) に分類し，その影響を受けた小花の割合を求めた．蛍光灯による日長処理（12時間，16時間，20時間，24時間）や白熱灯による日長処理（自然日長，24時間）は形態異常花序発生率に影響を及ぼさなかった．蛍光灯（PPFD 1μmol・m-2・s-1），白熱灯（PPFD 3μmol・m-2・s-1），メタルハライドランプ（PPFD 14μmol・m-2・s-1），高圧ナトリウムランプ（PPFD 48μmol・m-2・s-1）を用いて16時間の補光を行った．異なる光源による光強度でも形態異常発生率に一定の傾向は認められなかった．遮光時期を変えても形態異常発生率に一定の傾向は認められなかった． 最低夜温を15℃に上げると８℃区と比較して15℃区の形態異常発生は大きく減少した．特にパターン２と３の発生率は大幅に低下した．各実験の処理開始から発蕾までの平均夜温（7.1℃，9.0℃，9.2℃，11.6℃，16.4℃）と，パターン３の形態異常発生率（13.1%，8.7%，7.1%，1.1%，0.7%）との間に高い負の相関（R2＝0.849）が認められ，処理開始から発蕾までの平均夜温が高いほど形態異常発生率は低下した．以上のことから，形態異常花序発生には夜間の温度が大きく関与しているのではないかと推察された．
kn-abstract=As occurrence of abnormal inflorescence in Gypsophila paniculata ‘Altair’ is caused by environmental conditions, effects of day length, supplemental lighting strength, shading period and minimum night temperature on occurrence of abnormal inflorescence were investigated. Abnormal inflorescence was classified into four types : normal, pattern 1 (Short-flower stalk), pattern 2 (Coalescent two-flower stalk) and pattern 3 (Looping and irregular-flower stalk). Neither of 12h, 16h, 20h or 24h day length by fluorescent lamp, nor 24h by incandescent lamp affected occurrence of abnormal inflorescence. Effects of four levels of light intensity (fluorescent lamp : PPFD 1μmol・m−2・s−1, incandescent lamp : PPFD 3μmol・m−2・s−1, metal halide lamp : PPFD 14μmol・m−2・s−1 and high-pressure sodium lamp : PPFD 48μmol・m−2・s−1) were examined in 16h photoperiod. Occurrence of abnormal inflorescence was not affected by different light intensities, neither was it affected by shading period. Occurrence of abnormal inflorescence at 15°C was however significantly reduced compared to that at 8°C. In particular, patterns 2 and 3 at 15°C were significantly reduced compared to those at 8°C. There was a strong negative correlation between average night temperature from starting the treatment to flower budding (7.1°C, 9.0°C, 9.2°C, 11.6°C and 16.4°C) and incidence of pattern 3 (13.1%, 8.7%, 7.1%, 1.1% and 0.7%). Therefore, as average night temperature increased, occurrence of abnormal inflorescence decreased. The results show that low night temperature may be the main factor inducing occurrence of abnormal inflorescence.
en-copyright=
kn-copyright=

en-aut-name=YamaguchiNorihito
en-aut-sei=Yamaguchi
en-aut-mei=Norihito
kn-aut-name=山口訓史
kn-aut-sei=山口
kn-aut-mei=訓史
aut-affil-num=1
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=2
ORCID=

en-aut-name=KobikiKayoko
en-aut-sei=Kobiki
en-aut-mei=Kayoko
kn-aut-name=小日置佳世子
kn-aut-sei=小日置
kn-aut-mei=佳世子
aut-affil-num=3
ORCID=

en-aut-name=OtaniShoko
en-aut-sei=Otani
en-aut-mei=Shoko
kn-aut-name=大谷翔子
kn-aut-sei=大谷
kn-aut-mei=翔子
aut-affil-num=4
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=

affil-num=4
en-affil=
kn-affil=

affil-num=5
en-affil=
kn-affil=岡山大学
en-keyword=abnormal inflorescence pattern
kn-keyword=abnormal inflorescence pattern
en-keyword=cut flower form
kn-keyword=cut flower form
en-keyword=environmental factor
kn-keyword=environmental factor
en-keyword=incidence of abnormal inflorescence
kn-keyword=incidence of abnormal inflorescence
en-keyword=low night temperature
kn-keyword=low night temperature
END

start-ver=1.4
cd-journal=joma
no-vol=225
cd-vols=
no-issue=
article-no=
start-page=137
end-page=140
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201303
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=NMR study for electrochemically inserted Na in hard carbon electrode of sodium ion battery
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The state of sodium inserted in the hard carbon electrode of a sodium ion battery having practical cyclability was investigated using solid state 23Na NMR. The spectra of carbon samples charged (reduced) above 50 mAh g−1 showed clear three components. Two peaks at 9.9 ppm and 5.2 ppm were ascribed to reversible sodium stored between disordered graphene sheets in hard carbon because the shift of the peaks was invariable with changing strength of external magnetic field. One broad signal at about −9 to −16 ppm was assigned to sodium in heterogeneously distributed closed nanopores in hard carbon. Low temperature 23Na static and magic angle spinning NMR spectra didn't split or shift whereas the spectral pattern of 7Li NMR for lithium-inserted hard carbon changes depending on the temperature. This strongly suggests that the exchange of sodium atoms between different sites in hard carbon is slow. These studies show that sodium doesn't form quasi-metallic clusters in closed nanopores of hard carbon although sodium assembles at nanopores while the cell is electrochemically charged.
en-copyright=
kn-copyright=

en-aut-name=GotohKazuma
en-aut-sei=Gotoh
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IshikawaToru
en-aut-sei=Ishikawa
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ShimadzuSaori
en-aut-sei=Shimadzu
en-aut-mei=Saori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YabuuchiNaoaki
en-aut-sei=Yabuuchi
en-aut-mei=Naoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KomabaShinichi
en-aut-sei=Komaba
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakedaKazuyuki
en-aut-sei=Takeda
en-aut-mei=Kazuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=GotoAtsushi
en-aut-sei=Goto
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=DeguchiKenzo
en-aut-sei=Deguchi
en-aut-mei=Kenzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OhkiShinobu
en-aut-sei=Ohki
en-aut-mei=Shinobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=HashiKenjiro
en-aut-sei=Hashi
en-aut-mei=Kenjiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=ShimizuTadashi
en-aut-sei=Shimizu
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=IshidaHiroyuki
en-aut-sei=Ishida
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science & Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Department of Applied Chemistry, Tokyo University of Science

affil-num=3
en-affil=
kn-affil=Department of Applied Chemistry, Tokyo University of Science

affil-num=4
en-affil=
kn-affil=Department of Applied Chemistry, Tokyo University of Science

affil-num=5
en-affil=
kn-affil=Department of Applied Chemistry, Tokyo University of Science

affil-num=6
en-affil=
kn-affil=Division of Chemistry, Graduate School of Science, Kyoto University

affil-num=7
en-affil=
kn-affil=National Institute for Materials Science

affil-num=8
en-affil=
kn-affil=National Institute for Materials Science

affil-num=9
en-affil=
kn-affil=National Institute for Materials Science

affil-num=10
en-affil=
kn-affil=National Institute for Materials Science

affil-num=11
en-affil=
kn-affil=National Institute for Materials Science

affil-num=12
en-affil=
kn-affil=Graduate School of Natural Science & Technology, Okayama University
en-keyword=Sodium ion battery
kn-keyword=Sodium ion battery
en-keyword=Anode
kn-keyword=Anode
en-keyword=Hard carbon
kn-keyword=Hard carbon
en-keyword=23Na
kn-keyword=23Na
en-keyword=Solid state NMR
kn-keyword=Solid state NMR
END

start-ver=1.4
cd-journal=joma
no-vol=66
cd-vols=
no-issue=5
article-no=
start-page=369
end-page=376
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Dravet Syndrome : A Genetic Epileptic Disorder
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Dravet syndrome (DS), or severe myoclonic epilepsy in infancy, is one of the most severe types of genetic epilepsy. It is characterized by the initial occurrence of febrile or afebrile seizures that often evolve into status epilepticus in infants with normal development, and by the subsequent appearance of myoclonic and/or atypical absence seizures as well as complex partial seizures. The key feature that characterizes DS is fever sensitivity, although photosensitivity and pattern-sensitivity are also often seen. The prognosis is unfavorable in most cases. Seizures become drug-resistant and persist, with many patients suffering from motor and cognitive impairment. Mutations of SCN1A, which encodes the voltage-gated sodium channel NaV1.1, are the most frequent genetic cause of this syndrome. SCN1A mutations and/or microchromosomal rearrangements involving SCN1A are detected in about 85% of patients. Mutations of PCDH19 have also been reported in female patients with clinical findings compatible with DS. PCDH19 mutations might account for 5% of overall DS cases. Thirty years after its first description, DS is considered as a model of channelopathy. This survey reviews recent developments in the research literature on DS, focusing on the clinical course, as well as its genetic causes.
en-copyright=
kn-copyright=

en-aut-name=AkiyamaMari
en-aut-sei=Akiyama
en-aut-mei=Mari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KobayashiKatsuhiro
en-aut-sei=Kobayashi
en-aut-mei=Katsuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OhtsukaYoko
en-aut-sei=Ohtsuka
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Child Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, and Okayama University Hospital

affil-num=2
en-affil=
kn-affil=Department of Child Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, and Okayama University Hospital

affil-num=3
en-affil=
kn-affil=Department of Child Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, and Okayama University Hospital
en-keyword=Dravet syndrome
kn-keyword=Dravet syndrome
en-keyword=long-term outcome
kn-keyword=long-term outcome
en-keyword=SCN1A
kn-keyword=SCN1A
en-keyword=PCDH19
kn-keyword=PCDH19
END

start-ver=1.4
cd-journal=joma
no-vol=82
cd-vols=
no-issue=2
article-no=
start-page=1675
end-page=1683
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110301
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=An Efficient Synthesis of Antibiotic SF-2312 (3-Dihydroxyphosphoryl-1,5-dihydroxy-2-pyrrolidone)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=N-Benzyloxy-2-(diethoxyphosphoryl)pent-4-enamide (6) was prepared from ethyl diethoxyphosphorylacetate in a 3-step sequence. Oxidative cleavage of the terminal olefin of 6 with osmium tetroxide and sodium periodate afforded 1-benzyloxy-3-diethoxyphosphoryl-5-hydroxy-2-pyrrolidone (7). The first synthesis of racemic SF-2312 was achieved by treatment of 7 with trimethylsilyl bromide, followed by hydrogenolysis.
en-copyright=
kn-copyright=

en-aut-name=HanayaTadashi
en-aut-sei=Hanaya
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ItohChika
en-aut-sei=Itoh
en-aut-mei=Chika
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
en-keyword=Phosphonic Acid
kn-keyword=Phosphonic Acid
en-keyword=Hydroxamic Acid
kn-keyword=Hydroxamic Acid
en-keyword=Antibiotic
kn-keyword=Antibiotic
en-keyword=2-Pyrrolidone
kn-keyword=2-Pyrrolidone
en-keyword=C-P Bond
kn-keyword=C-P Bond
END

start-ver=1.4
cd-journal=joma
no-vol=124
cd-vols=
no-issue=2
article-no=
start-page=115
end-page=117
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120801
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Therapy for hyperthermia-induced seizures in Scn1a mutant rats
kn-title=Scn1a遺伝子変異ラットを用いた抗てんかん薬の熱性けいれん抑制効果
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=HayashiKeiichiro
en-aut-sei=Hayashi
en-aut-mei=Keiichiro
kn-aut-name=林桂一郎
kn-aut-sei=林
kn-aut-mei=桂一郎
aut-affil-num=1
ORCID=

en-aut-name=OhmoriIori
en-aut-sei=Ohmori
en-aut-mei=Iori
kn-aut-name=大守伊織
kn-aut-sei=大守
kn-aut-mei=伊織
aut-affil-num=2
ORCID=

en-aut-name=MatsuiHideki
en-aut-sei=Matsui
en-aut-mei=Hideki
kn-aut-name=松井秀樹
kn-aut-sei=松井
kn-aut-mei=秀樹
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　細胞生理学

affil-num=2
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　細胞生理学

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　細胞生理学
en-keyword=febrile seizures
kn-keyword=febrile seizures
en-keyword=animal model
kn-keyword=animal model
en-keyword=Scn1a gene
kn-keyword=Scn1a gene
en-keyword=generalized epilepsy with febrile seizures plus
kn-keyword=generalized epilepsy with febrile seizures plus
en-keyword=severe myoclonic epilepsy of infancy
kn-keyword=severe myoclonic epilepsy of infancy
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=7
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Effect of Prevention of Root Injury on Growth, Development and Dry Weight in Rice (Oryza sativa L.) Transplanted to Saline Soil
kn-title=根損傷の軽減が塩土壌における移植イネの生育と乾物重におよぼす影響
en-subtitle=
kn-subtitle=
en-abstract=イネの根は吸水するとともにナトリウムイオンを排除する役割を持っている．水田移植栽培において根の損傷を防ぐことが効果的に塩害を軽減するかどうかを検討した．耐塩性の弱いイネ極早生品種アキヒカリを用い，土壌を詰めたビニール袋で育苗した．苗の根を5 ㎜～10 ㎜ 残し切除して，塩化ナトリウム（NaCl）0ｇ，7ｇ，10ｇを土壌に添加したポットに移植し，根を傷つけないように移植した植物と生育と成熟期の乾物重を比較した．移植は播種後12日，19日，26日，33日の4回行った．茎数の増加は根の切除および塩添加によって抑制されたが，二要因の交互作用はなかった．成熟期の地上部と穂の乾物重は塩添加によって減少したが，根切除の影響はなかった．穂の乾物重の減少は穂数と平均1籾重が変わらなかったため，平均1穂籾数の低下による平均1穂重の低下によっていた．以上の結果から耐塩性が小さい日本の品種において移植時の根の損傷を防ぐことは，塩害軽減に効果的でないと考えられた．
kn-abstract=Rice root has an important role in water absorption and exclusion of sodium ion in saline soil. However, it is injured during transplanting to paddy field. The objective of this study was to examine whether prevention of such root injury reduces salinity damage in rice. Extremely early rice cultivar "Akihikari" that is not salt tolerant was grown in plastic bags filled with soil. Roots of seedlings were clipped leaving 5mm~10mm, then transplanted to 5L pots filled with soil applied with 0g, 7g and 10g of sodium chloride (NaCl). The growth and dry weight at the mature stage was compared to those of plants transplanted with intact roots. Time of transplanting was 12, 19, 26 and 33 days after sowing. Increase of number of stems was suppressed due to root clipping and salinity, but there was no interaction between the two factors. Dry weight of total above ground part and panicle decreased due to salinity though root clipping did not change them. The reduction of panicle dry weight was caused by the reduction of mean number of spikelets per panicle, thus mean panicle dry weight, as number of panicles and mean spikelet dry weight showed no changes. It was concluded that prevention of the root injury may not effectively alleviate salinity damage in less salt tolerant rice cultivars.
en-copyright=
kn-copyright=

en-aut-name=TsudaMakoto
en-aut-sei=Tsuda
en-aut-mei=Makoto
kn-aut-name=津田誠
kn-aut-sei=津田
kn-aut-mei=誠
aut-affil-num=1
ORCID=

en-aut-name=UedaYukie
en-aut-sei=Ueda
en-aut-mei=Yukie
kn-aut-name=上田裕紀枝
kn-aut-sei=上田
kn-aut-mei=裕紀枝
aut-affil-num=2
ORCID=

en-aut-name=HiraiYoshihiko
en-aut-sei=Hirai
en-aut-mei=Yoshihiko
kn-aut-name=平井儀彦
kn-aut-sei=平井
kn-aut-mei=儀彦
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=Rice (Oryza sativa L.)
kn-keyword=Rice (Oryza sativa L.)
en-keyword=Root clipping
kn-keyword=Root clipping
en-keyword=Salinity
kn-keyword=Salinity
en-keyword=Tillering
kn-keyword=Tillering
en-keyword=Transplantation
kn-keyword=Transplantation
END

start-ver=1.4
cd-journal=joma
no-vol=123
cd-vols=
no-issue=3
article-no=
start-page=197
end-page=206
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20111201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A new paradigm for the treatment of lifestyle-related diseases : Microinflammation as a novel therapeutic target
kn-title=生活習慣病治療のパラダイムシフト―慢性炎症を標的とした治療戦略―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=ShikataKenichi
en-aut-sei=Shikata
en-aut-mei=Kenichi
kn-aut-name=四方賢一
kn-aut-sei=四方
kn-aut-mei=賢一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学病院　新医療研究開発センター
en-keyword=生活習慣病
kn-keyword=生活習慣病
en-keyword=メタボリック症候群
kn-keyword=メタボリック症候群
en-keyword=糖尿病
kn-keyword=糖尿病
en-keyword=炎症
kn-keyword=炎症
en-keyword=心血管疾患
kn-keyword=心血管疾患
END

start-ver=1.4
cd-journal=joma
no-vol=118
cd-vols=
no-issue=1379
article-no=
start-page=603
end-page=607
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201007
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Phase separation of borosilicate glass containing sulfur
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A 10Na(2)S·30B(2)O(3)·60SiO(2) (mol %) glass was prepared, and the changes in glass structure and chemical state of sulfur caused by phase separation were investigated. In the as-prepared and heat-treated glasses, sulfur was present as S(2)− anion and polysulfide S(2)− and S(3)− anions, and Si–S and B–S bonds were not confirmed. A phase separation by spinodal decomposition was observed after heat-treatment, where sulfur was preferentially distributed to borate-rich phase. Even after the phase separation, formation of non-bridging oxygen was not recognized. The preferential distribution of sulfur anions in the present glass was explainable on the basis of the change in population of sodium ions, which compensated the negatively-charged sulfur anions.
en-copyright=
kn-copyright=

en-aut-name=SaikiKeiji
en-aut-sei=Saiki
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SakidaShinichi
en-aut-sei=Sakida
en-aut-mei=Shinichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=BeninoYasuhiko
en-aut-sei=Benino
en-aut-mei=Yasuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NanbaTokuro
en-aut-sei=Nanba
en-aut-mei=Tokuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University

affil-num=2
en-affil=
kn-affil=Environmental Management Center, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University

affil-num=4
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University
en-keyword=Phase separation
kn-keyword=Phase separation
en-keyword=Borosilicate glass
kn-keyword=Borosilicate glass
en-keyword=Chemical state of sulfur
kn-keyword=Chemical state of sulfur
en-keyword=Glass structure
kn-keyword=Glass structure
END

start-ver=1.4
cd-journal=joma
no-vol=15
cd-vols=
no-issue=3
article-no=
start-page=403
end-page=410
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=201105
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Characterization of an OmpA-like outer membrane protein of the acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=An OmpA family protein (FopA) previously reported as one of the major outer membrane proteins of an acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans was characterized with emphasis on the modification by heat and the interaction with peptidoglycan. A 30-kDa band corresponding to the FopA protein was detected in outer membrane proteins extracted at 75A degrees C or heated to 100A degrees C for 10 min prior to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). However, the band was not detected in outer membrane proteins extracted at a parts per thousand currency sign40A degrees C and without boiling prior to electrophoresis. By Western blot analysis using the polyclonal antibody against the recombinant FopA, FopA was detected as bands with apparent molecular masses of 30 and 90 kDa, suggesting that FopA existed as an oligomeric form in the outer membrane of A. ferrooxidans. Although the fopA gene with a sequence encoding the signal peptide was successfully expressed in the outer membrane of Escherichia coli, the recombinant FopA existed as a monomer in the outer membrane of E. coli. FopA was detected in peptidoglycan-associated proteins from A. ferrooxidans. The recombinant FopA also showed the peptidoglycan-binding activity.
en-copyright=
kn-copyright=

en-aut-name=ManchurMohammed Abul
en-aut-sei=Manchur
en-aut-mei=Mohammed Abul
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KikumotoMei
en-aut-sei=Kikumoto
en-aut-mei=Mei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KanaoTadayoshi
en-aut-sei=Kanao
en-aut-mei=Tadayoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakadaJun
en-aut-sei=Takada
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KamimuraKazuo
en-aut-sei=Kamimura
en-aut-mei=Kazuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

affil-num=4
en-affil=
kn-affil=Division of Chemical and Biological Technology, Graduate School of Natural Science and Technology, Okayama University

affil-num=5
en-affil=
kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
en-keyword=Acidithiobacillus ferrooxidans
kn-keyword=Acidithiobacillus ferrooxidans
en-keyword=Iron-oxidizing bacterium
kn-keyword=Iron-oxidizing bacterium
en-keyword=Acidophile
kn-keyword=Acidophile
en-keyword=Outer membrane protein
kn-keyword=Outer membrane protein
en-keyword=OmpA
kn-keyword=OmpA
END

start-ver=1.4
cd-journal=joma
no-vol=123
cd-vols=
no-issue=2
article-no=
start-page=91
end-page=95
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110801
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A long-term follow-up study of Dravet syndrome up to adulthood
kn-title=成人期に達したDravet症候群の長期経過に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=AkiyamaMari
en-aut-sei=Akiyama
en-aut-mei=Mari
kn-aut-name=秋山麻里
kn-aut-sei=秋山
kn-aut-mei=麻里
aut-affil-num=1
ORCID=

en-aut-name=KobayashiKatsuhiro
en-aut-sei=Kobayashi
en-aut-mei=Katsuhiro
kn-aut-name=小林勝弘
kn-aut-sei=小林
kn-aut-mei=勝弘
aut-affil-num=2
ORCID=

en-aut-name=YoshinagaHarumi
en-aut-sei=Yoshinaga
en-aut-mei=Harumi
kn-aut-name=吉永治美
kn-aut-sei=吉永
kn-aut-mei=治美
aut-affil-num=3
ORCID=

en-aut-name=OhtsukaYoko
en-aut-sei=Ohtsuka
en-aut-mei=Yoko
kn-aut-name=大塚頌子
kn-aut-sei=大塚
kn-aut-mei=頌子
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　発達神経病態学

affil-num=2
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　発達神経病態学

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　発達神経病態学

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　発達神経病態学
en-keyword=Dravet症候群
kn-keyword=Dravet症候群
en-keyword=乳児重症ミオクロニーてんかん
kn-keyword=乳児重症ミオクロニーてんかん
en-keyword=予後
kn-keyword=予後
en-keyword=けいれん性てんかん重積状態
kn-keyword=けいれん性てんかん重積状態
END

start-ver=1.4
cd-journal=joma
no-vol=40
cd-vols=
no-issue=4
article-no=
start-page=717
end-page=726
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1928
dt-pub=19280430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=利尿劑竝ニ其他ノ藥劑ノ腎臟絲毬體ニ及ボス作用ニ就テ
kn-title=On the action of some diuretica and other drugs upon the glomeruli
en-subtitle=
kn-subtitle=
en-abstract=利尿劑ノ利尿作用ハ絲毬體ニ於ケル濾過作用ヲ亢進スルカ細尿管ノ吸收ヲ抑制スルカ或ハ此兩者ヲ兼ヌルニ在リ,而シテ血管毬(絲毬體)ノ濾過ラ亢進スルモノハ腎動脉系ノ血壓上騰,腎臟ニ於ケル血液灌流ノ速度及ビ血液中水分ノ増大即チ水血症ナリ果シテ利尿劑ハ絲毬體ニ對シ如何ナル影響ヲ及ボスモノナルヤヲ見ント欲シ次ノ實驗ヲ行ヘリ. 古來腎臟ニ對スル藥品ノ作用ハ灌流試驗又ハOncometerヲ以テ行ハルルヲ常トスルモ此場合カカル方法ニテハ腎絲毬體ニ作用スル結果ナルヤ又腎臟動脉ニ作用スルモノナルヤヲ判別スル事難キラ以テ予ハ腎臟ヲ露出セシメ絲毬體ノ状態ヲ顯微鏡下ニ觀察シツツ豫メ造リタル硝子毛細管ヲ用ヒ各種利尿劑及ビ其他ノ藥液ヲ滴下シツツ絲毬體ノ變化ヲ觀察セリ而シテ次ノ成績ヲ得タリ.「カフエイン」安息香酸「ナトリウムカフエイン」「ヂウレチン」及ビ「クレアチン」ハ絲毬體毛細血管ヲ擴大セシメ爲ニ絲毬體内ノ血流ハ早クナリ血量ハ増加シ絲毬體ハ充血且膨大ス今迄見エザリシ絲毬體ハ出現シ活動盛トナル.之ニ反シ醋剥液ハ0.1%ニ於テ既ニ絲毬體毛細血管ヲ收縮セシメ活動セル絲毬體ハ靜止シ漸次其數ヲ減ズ.「テオチン」「葡萄糖」ハ何等ノ影響ヲ及ボサズ.
Guanidinハ稀薄液ニ於テハ絲毬體毛細血管ヲ擴大シ其數ヲ増加セシムルモ濃厚液ニ於テハ毛細管ハ收縮シ絲毬體ハ消失ス,「ナトリウム」鹽類中(0.6%ノRinger氏液ト等滲透性液ニ於テ) NaNO(2), NaNO(3)及ビNa(2)HPO(4)ハ輕度ニ毛細管ヲ(絲毬ノ)擴大セシムルモ其他ハ何等ノ影響ナシ.「カリウム」鹽類ハ0.1%ノ稀薄液ニ於テ絲毬體毛細血管ヲ收縮セシメ絲毬體ハ活動ヲ停止シ遂ニ全ク消失スルニ至ル.「カルチウム」鹽類「マグネシウム」鹽類竝ニ二三有機酸ハ共ニ絲毬體毛細血管ヲ擴大セシム絲毬體ハ充血大ス靜止ノ状態ニ在リシ絲毬體ハ活動ヲ初メ其數ヲ増加ス.
kn-abstract=From the results of my experiments the conclusion may be summarized as follows. 1. Caffeine, caffeine sodio-benzoate and diuretin have dilator effect upon the glomerular capillaries and the circulation in them becomes more rapid. The quantity of blood which flows through them increases and a number of glomeruli, hitherto unobserved, become visible. On the other hand, theocin and glucose have no effect upon the glomeruli. 2. Solution of potassium acetate causes a remarkable contraction and at the same time the glomeruli till then observed, become invisible. 3. Kreatin produces a dilatation of glomerular capillaries and accordingly the circulation in them becomes more rapid and also the number of glomeruli increased. Guanidin in a solution weaker than 0.4 p. c. causes dilatation of glomerular capillaries and increase of the quantity of blood which flows through them and also it causes the increase of verocity of blood flow in the glomerular capillaries. The glomeruli, hitherto stationary, become active and the number of them increases. On the contrary, a solution stronger than 0.5 p. c. always causes contraction of glomerular capillaries and the number of glomeruli are decreased. 4. Isotonic solution (with 0.6% Ringer's solution) of NaNO(2), NaNO(3) and Na(2)HPO(4) causes a slight dilatation of glomerular capillaries and accordingly the increase of verocity of blood flow in them and of quantity of blood flowing through them are produced. But other sodium salts have no effect upon the glomeruli. All of potassium salts, on the contrary, have an opposite effect upon the glomeruli to that of sodium salts. 5. Calcium salts and magnesium salts (isotonic with 0.6% Ringer's solution) and some organic substances (m/100 in density) have a remarkable dilator effect upou the glomerular capillaries and cause the increase of verocity of blood flow in them and of quantity of blood which flows through them. Glomeruli are increased in number und are remarkably congested and swollen.
en-copyright=
kn-copyright=

en-aut-name=OkadaMasanori
en-aut-sei=Okada
en-aut-mei=Masanori
kn-aut-name=岡田正矩
kn-aut-sei=岡田
kn-aut-mei=正矩
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=physiological Department of University, Okayama
END

start-ver=1.4
cd-journal=joma
no-vol=44
cd-vols=
no-issue=5
article-no=
start-page=1530
end-page=1552
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1932
dt-pub=19320531
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A Contribution to the Pharmacology of Ornithuric Acid
kn-title=「オルニツール」酸ノ藥理學的作用ニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The pharmacological action of phenol-glycuronic acid, urochloralic acid, glycuronic acid and benzoyl-glycuronic acid have been investigated in our Laboratory by Ishii, Matsushima, Tanaka and Sakata. Following the line of these investigations the author has studied the pharmacological action of ornithuric acid prepared from the urine of hen by giving sodium benzoate. The results obtained are as follows: (1) Ornithuric acid when given to the rabbit in its general circulation gives rise to a marked diuresis. Meanwhile it causes practically no change in general blood pressure, nor in the water content of the blood, and only the oncometric record shows an augmentation of kidney volume in every case. And in denervated or isolated kidney an increased perfusion rate is obtained. So it seems that in this vasomotor reaction the central nervous mechanism plays no part. (2) Ornithuric acid exerts diuretic action also on cold blooded animal. Analysis effected on perfused toad kidney showed that it concerns in this diuresis the glomerular system only, and that tubular system has nothing to do with. (3) The action of ornithuric acid on the blood vessel of the organs other than kidney is as follows: - Hind leg vascular system of the frog is dilated by low and constricted by high concentration of this substance in the perfusing fluid. Vascular system of the rabbit's ear shows similar reaction toward this substance. But coronary vascular system is constricted notwithstanding the concentration of this substance in the perfusing fluid. (4) Isolated hearts of toad and rabbit augment their tone and diminish the amplitude of their contraction by middle dosage and especially in the beginning, but then tone returns gradually to normal, accompanying an increase in the amplitude of contraction. By larger dosages there occurs an increase in amplitude of the heart beat during only a very short period at the beginning, then the amplitude decreases progressively to be finally arrested at systole. It must be noted that in every case there is no change in the frequency of heart beat. (5) In isolated smooth muscular organs of the rabbit, such as intestine, uterus and bladder, an augmentation in the tone and the spontaneous movement are observed for an appropriate dosage. Atropine has no influence on this effect, also the isolated trigonal and basal parts of the rabbit's bladder have shown no difference in their reaction toward this substance. (6) From these upper three results it may be concluded that ornithuric acid does not exert its action by way of the nervous mechanism but directly on the smooth muscular system.
en-copyright=
kn-copyright=

en-aut-name=NakanoMitsutaka
en-aut-sei=Nakano
en-aut-mei=Mitsutaka
kn-aut-name=中野滿隆
kn-aut-sei=中野
kn-aut-mei=滿隆
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=大阪帝國大學醫學部藥理學教室
END

start-ver=1.4
cd-journal=joma
no-vol=46
cd-vols=
no-issue=12
article-no=
start-page=3065
end-page=3073
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1934
dt-pub=19341231
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Value of Trental (or Tonophosphan) in Rickets. An Experimental Study.
kn-title=實驗的家兎佝僂病ニ對スル「トレンタール」ノ作用ニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Experiments were undertaken to determine the value of Trental, a sodium salt of dimethylamino-methylphenyl phosphoric acid, in rickets of young growing rabbits. The standard rickets-producing dietary was fed and, as a supplement, Trental was given subcutaneously. All the control animals which were fed the rickets-producing diet, without exception developed rickets. In the large series of animals received Trental developed rickets in somewhat slighter degree and showed a slightly better increase of both body weight and bone length than that of the control animals, although in the small series of the animals developed rickets to the same extent as the control animals.
en-copyright=
kn-copyright=

en-aut-name=OkumuraMasanobu
en-aut-sei=Okumura
en-aut-mei=Masanobu
kn-aut-name=奥村雅延
kn-aut-sei=奥村
kn-aut-mei=雅延
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學小兒科教室
END

start-ver=1.4
cd-journal=joma
no-vol=47
cd-vols=
no-issue=4
article-no=
start-page=1069
end-page=1093
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1935
dt-pub=19350430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the Action of Electric Current upon Organisms (3rd. Report.) On the Alternation of Permeability of Cell-membranes by Electric Current
kn-title=電流ノ生物ニ對スル作用ニ就テ（第3報）電流刺戟ニヨル細胞膜ノ透過性ノ變化ニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The auther studied on alternation of permeability of cell-membranes by electric current and reached to the following results. 1) Plasmolysis of epithelial cells of Tradescantia virginica by hypertonic solution (0.5-1.0%) of sodium chloride will be stopped at the neighbourhood of the cathode by passing through of the electric current. 2) In the neighbourhood of cathode potassium-ions escape from the cells into the surrounding medium. It will be so understanded that the damage of semipermeability of the cell-membranes allows passage of anion and potassium-ion escapes in balance of it. Same thing occurs on the muscle cutaneus dorsi of frog. 3) Potassium-escape can be observed on the resting muscle of frog when the temperature of bathing fluid exceeds 29-32°C.
en-copyright=
kn-copyright=

en-aut-name=MoriNobutane
en-aut-sei=Mori
en-aut-mei=Nobutane
kn-aut-name=森信胤
kn-aut-sei=森
kn-aut-mei=信胤
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學生理學教室
END

start-ver=1.4
cd-journal=joma
no-vol=52
cd-vols=
no-issue=7
article-no=
start-page=1664
end-page=1674
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1940
dt-pub=19400731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the Diuretic Action of the Extractum of Radicis Phytolaccae (Phytolacca esculenta), Extracts of several Plants and some Drugs. (1. Part.) On the Diuretic Action of the Extractum of Radicis Phytolaccae (Phytolacca esculenta).
kn-title=各種植物及ビ2, 3藥物ノ利尿作用ニ就テ（第1報）「商陸エキス」ノ利尿作用ニ就テ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By the perfusion method through the kidney of the toad following results were obtained. 1) When the Extr. of Rad. Phytol. is added to the perfusing fluid, it increases distinctly the flow of urine. 2) Conversely, the addition of potassium salt, which will be contained abundantly in Rad. Phytol., brings, firstly, the remarkable decrease of the urine flow, and then it may increase slightly. 3) Ash of the Rad. Phytol. dissolved in Ringer's solution decreases the urine flow remarkably at first, and then it may increase. 4) When the Extr. of Rad. Phytol. is applied in drops to the exposed kidney of frog, causes dilatation of glomerular capillaries; the circulation in them becomes more rapid, i.e. the quantity of blood which flows through them increases, and a number of glomeruli, hitherto invisible, becomes visible. The application of this extract in drops on the web of frog dilates its blood-capillaries and increases the blood-flows through them. 5) But the solution of potassium salt used, or the ash of the Rad. Phytol. causes contraction of glomerular capillaries and capillaries of web of frog. The blood-flows in the capillaries become remarkably slow, i.e. the quantity of blood which flows through them decreases. The glomeruli, hitherto activ, becomes stationary and visible number of glomeruli diminishes. 6) The measurement of the freezing-point revealed that the osmotic pressure of the Extr. of Rad. Phytol. used was not very different from the normal Ringer's solution. 7) By the flame-reaction it was ascertained that potassium and sodium salts were contained in the Rad. Phytol. 8) Diuretic action of Rad. Phytol. is due to some organic substance, which causes dilatation of capillaries of glomerulus and web of frog. Potassium salt which is contained in Rad. Phytol. makes some additional action through hydraemia.
en-copyright=
kn-copyright=

en-aut-name=MasuzawaHiromu
en-aut-sei=Masuzawa
en-aut-mei=Hiromu
kn-aut-name=益澤博
kn-aut-sei=益澤
kn-aut-mei=博
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學生理學教室
END

start-ver=1.4
cd-journal=joma
no-vol=54
cd-vols=
no-issue=8
article-no=
start-page=1430
end-page=1448
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1942
dt-pub=19420831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Acetyocholine and Choline Esterase (The Third Report.) Action of some Narcotics on Choline Esterase and Influence of these on Acetylcholine Content in the central Nervous System
kn-title=Acetylcholin及ビ抗Cholinesterase劑ノ作用機轉補遺（其ノ3）諸種麻醉藥ノCholinesteraseニ對スル作用 附 諸種麻醉藥ノ腦髓竝ニ脊髓A.Ch.含有量ニ及ボス影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author had studied the action of some narcotics (urethane, chloralhydrate, barbital, luminal-Na, sulfonal, morphine hydrochl., ether and chloroform) on choline esterase and the influence of these on acetylcholine content in the central nervous system and the result is as follows: 1) These drugs inhibited the action of choline esterase. 2) The intensity of the action of these drugs (except ether and chroloform) on esterase had following order, i.e. morphine>barbital>chloralhydrate>sulfonal> urethaue>luminal. Except of sodium salt of luminal the action of those drugs is approximately proportional to their molecular weight. 3) The relation of the inhibitory action between these drugs (added luminal, brovarin) and choline esterase is linear. 4) The acetylcholine content in the central nervous system of the mouse was increased by these drugs.
en-copyright=
kn-copyright=

en-aut-name=OkudaKazumasa
en-aut-sei=Okuda
en-aut-mei=Kazumasa
kn-aut-name=奧田一誠
kn-aut-sei=奧田
kn-aut-mei=一誠
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學生理學教室
END

start-ver=1.4
cd-journal=joma
no-vol=55
cd-vols=
no-issue=3
article-no=
start-page=429
end-page=436
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1943
dt-pub=19430331
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Micromethod for the Rapid Determination of Specific Gravity
kn-title=微量物質ノ比重測定1新法
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The method here described is an improvement of that developed by LinderstrømLang, Lanz and Jacobsen (1938, 1940). A reasonably linear specific gravity gradient is produced in a vertical measuring cylinder by mixing kerosene and bromobenzene, or benzene and chroloform in varying proportions. In kerosene-bromobenzene mixtures the position of a drop is determined after the drop (diameter 2.0mm) has fallen in 30 sec. Plotting the positions of drops of known solutions of sodium chloride as ordinates against the corresponding specific gravities as abscissae, a nearly straight line is obtained in a coordinate system, from which it is possible, knowing the position of the drop of the unknown solution, to read the specific gravity of the solution with considerable accuracy. The reading of the positions of drops may be done with the naked eye making use of two sheeves of section papers, each of which are stick on at the diametral wall of measuring cylinder. The eye is kept at the level of the drop and the same position of two scale divisions, in order to eliminate errors due to parallaxis. In benzene-chloroform mixtures the drops fall more sapid, and come to rest much faster, so that the infusion of the mixtures into the drops will be minimized. At the constant temperature it is not advisable to use of kerosene-bromobenzene mixtures for more than about 1 hour at one experiment, and to use of benzene-chloroform mixtures for more than about 2 hours. Therefore, benzene-chloroform mixtures are more suitable than kerosene-bromobenzene mixtures for the determination of specific gravity. Its accuracy is 0.1 per cent.
en-copyright=
kn-copyright=

en-aut-name=GesiTakamaro
en-aut-sei=Gesi
en-aut-mei=Takamaro
kn-aut-name=下司孝麿
kn-aut-sei=下司
kn-aut-mei=孝麿
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山醫科大學生理學教室
END

start-ver=1.4
cd-journal=joma
no-vol=66
cd-vols=
no-issue=5
article-no=
start-page=909
end-page=918
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1954
dt-pub=19540531
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The experimental study of Colibacteriophage Report I Chromatograph of Colibacteriophage
kn-title=大腸菌ファージの実験的研究 第一編 大腸菌ファージのクロマトグラフ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Since 1940 the study of phage opened not only the beginning of co-operate study for physicists and chemists, but also the phage came to be clearified its character as the Bacterio Virus gradually. Then the study of phage made not a few contributions to the development of the study of Virus as model material of its kind. Now, these results are due to the endeavors of such scholars as Delbrück, Anderson, Cohen, Putznam, Doermann, Wyckoff and their co-operators mainly. Then, Charles. C. Shepard found out the characteristics of a distinctive nature in paperstrips in the course of investigations for the adsorptive behavior of Viruses. I tried some experiments in the paperchromatograph and capillarglass-chromatograph about the conditions that the phagef of coli-strains being adsorbed and recovered by paper and capillarglass. 1. The solvent employed here was 0.1% bovine serum albumin in 0.1 M sodium chloride. 2. The solution employed was colibacteriophage diluted appropriately in the same solvent. 3. The adsorbent employed was Toyo-filterpaper and capillarglass which were treated previously by 0.01 M hydrochloric acid and then washed by glasswather. 4. The experiments were carried out in an ice-box. At the beginning of an experiment a paperstrip was placed in the test tube so that it might not touch the sides, but touch the 20 ml. of solvent placed beforehand in the bottom of the tube. When the water front passed the second 1.25 cm section, 0.002 ml. of phage diluted in the same solvent appropriately as was placed in the bottom of the tube was quickly placed in the center of the second section and. the paper returned to the position in the tube.
When the water front passed the last marked section, the paper was removed from the tube and ten marked sections were quickly cut off into their respective ten test tubes each containing of a culture of E. coli-strains. For a period of not more than 10 minutes, the ten sections of paper were stirred occasionally and pressed against the sides of the tubes. At the end of it, 0.5 ml. were taken out and added it to 3.0 ml. of 0.7% agar; the resultant 3.5 ml. were mixed up and poured on a 1%, agarplate. There was recovered viable phage in a considerable degree. 5. It seems to me that this recoveries of phage has been carried out due to the presence of bovine serum albumin. The chromatograph conducted here would seem to involbe the mixture of elution and displacement in the Terminology of Tiselius.
en-copyright=
kn-copyright=

en-aut-name=SasakiK.
en-aut-sei=Sasaki
en-aut-mei=K.
kn-aut-name=佐々木峻
kn-aut-sei=佐々木
kn-aut-mei=峻
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部衛生学教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=10
article-no=
start-page=2505
end-page=2510
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19571031
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Action of Various Inhibitors and Antibiotics on Glutamate-Respiration of Staphylococcus
kn-title=ブドウ球菌のグルタミン酸呼吸に対する諸種阻害剤及び抗生物質の作用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author studied the action of various inhibitors and antibiotics on the glutamaterespiration of staphylococci under the consideration of physiological structure of the cell. Staphylococcus citreus and aureus (Terashima) were used as the test organisms, and L-glutamic acid as the substrate. The results were as follows: 1) Thionine, sodium arsenite and 8-hydroxyquinoline inhibited the respiration of intact cells of staphylococci markedly, but did not inhibit that of cell-free extracts. The inhibitive action of these three sorts of inhibitors was stronger on Staph. aureus (Terashima) than on Staph. citreus. 2) In intact cells, the inhibition of respiration by potassium cyanide and sodium azide was not restored by addition of thionine. In cell-free extracts, however, the inhibition by these two sorts of inhibitors was well restored by thionine. 3) By addition of thionine, the inhibition of the respiration of cell-free extracts by octanole was not so well restored as that by cyanide or azide. 4) 2, 4-Dinitrophenol inhibited the glntamate-respiration of both of the intact cells and cell-free extracts. 5) Of all the antibiotics tested, aureomycin was the only one which noticeably inhibited the glutamate-respiration of staphylococci.
en-copyright=
kn-copyright=

en-aut-name=YabeYoshiro
en-aut-sei=Yabe
en-aut-mei=Yoshiro
kn-aut-name=矢部芳郎
kn-aut-sei=矢部
kn-aut-mei=芳郎
aut-affil-num=1
ORCID=

en-aut-name=AkitaKazuo
en-aut-sei=Akita
en-aut-mei=Kazuo
kn-aut-name=秋田和男
kn-aut-sei=秋田
kn-aut-mei=和男
aut-affil-num=2
ORCID=

en-aut-name=AkitaYoshimi
en-aut-sei=Akita
en-aut-mei=Yoshimi
kn-aut-name=秋田悦示
kn-aut-sei=秋田
kn-aut-mei=悦示
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部微生物学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=7
article-no=
start-page=1765
end-page=1771
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental studies on the glycolysis and tissue respiration in the brain of chronic cerebral local anaphylactic rabbits Part III. On the glycolysis of the brain of chronic cerebral local anaphylactic rabbits in irrigation method
kn-title=慢性脳局所アナフィラキシー家兎脳髄の解糖作用ならびに組織呼吸に関する研究 第3編 慢性脳局所アナフィラキシー家兎脳髄の灌流実験法による糖代謝に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The glycolysis of the living brain of deacapitated chronic cerebral local anaphylactic rabbits was investigated by irrigation method.
Glucose was added to the irrigating fluid and the consumption of glucose was measured. In the chronic cerebral local anaphylactic rabbits the glucose-consumption was markedly strained in comparison with that in normal group. The pyruvic acid increased in both anaphylactic and normal groups, but the anaphylactic group showed a less increase than the normal. The addition of sodium pyruvate to the irrigating fluid caused less decrease of pyruvic acid in chronic cerebral anaphylactic rabbits than that in normal group From the facts above mentioned it is considred, that glycolysis is strained in the brain of chronic erebral local anaphylactic rabbits.
en-copyright=
kn-copyright=

en-aut-name=OboYoshio
en-aut-sei=Obo
en-aut-mei=Yoshio
kn-aut-name=於保義雄
kn-aut-sei=於保
kn-aut-mei=義雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=3
article-no=
start-page=947
end-page=958
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570331
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental and Clinical Studies on Parathionpoisoning Part 3. The Effectiveness of SH Medical Compounds and PAM on Parathion Poisoning - especially on Protein Active SH Radical and Mucoprotein of Serum -
kn-title=パラチオン中毒に関する実験的並びに臨床的研究 第三編 パラチオン中毒治療剤と血清蛋白活性SH基及びムコ蛋白
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=SH medical compounds used in this study were BAL, sodium thiosulfate, cystine, menthionine and sodium mercaptoacetate. 1. In the animal tests on the effectiveness of SH medical compounds, BAL was the most effective and then sodium thiosulfate was next, while scarecely effect was observed in the experiments of cystine, methionine and sodium mercaptoacetate. 2. By the administration of PAM, SH enzyme such as mucoprotein was promptly and certainly reactivated as well as cholinesterase.
en-copyright=
kn-copyright=

en-aut-name=HukuharaArimitsu
en-aut-sei=Hukuhara
en-aut-mei=Arimitsu
kn-aut-name=福原有光
kn-aut-sei=福原
kn-aut-mei=有光
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=2
article-no=
start-page=549
end-page=560
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Glutamic Acid Metabolism of Bacteria II: Glutamic acid metabolism of Staphylococcus albus
kn-title=細菌のグルタミン酸代謝に関する研究 第二篇 白色ブドウ球菌のグルタミン酸代謝
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=As to the physiological or metabolic features of pathogenic staphylococci, there are only a few reports, compared with those of Escherichia coli which was treated in report I. In this report, the author reports about the physiological aspects, particularly about the terminal respiratory system of Staphylococcus albus, entering from the studies of glutamic acid metabolism: 1) Staph. albus has the so-called citric acid cycle as its terminal respiratory ststem. 2) As a result of oxidative deamination, glutamic acid enters into the citric acid cycle and is further oxidized through this cycle. Glutamic acid is, however, best oxidized of all the intermediates of citric acid cycle and the related compounds. 3) Glutamic-aspartic and glutamic-alanic transaminations are carried out by this organism, in which glutamic acid plays the central role. 4) Divalent metal ions (Mg(++), Mn(++) and Fe(++)) show no remarkable effect on the glutamate-respiration of Staph. albus. 5) Of the various inhibitors tested, sodium azide, 2: 4-dinitrophenol, sodium arsenite and 8-hydroxyquinoline inhibit the glutamate-respiration strongly, and the most remarkable is the inhibitive action of 8-hydroxyquinoline. 6) Of the various antibiotics used, the inhibitive action of aureomycin is the most remarkable. Penicillin also shows some inhibitive action at pH 5.4. 7) The inhibition of the glutamate-respiration of this organism by these various inhibitors and antibiotics shows usually the tendency to rise up in the region of lower pH.
en-copyright=
kn-copyright=

en-aut-name=AkitaYoshimi
en-aut-sei=Akita
en-aut-mei=Yoshimi
kn-aut-name=秋田悦示
kn-aut-sei=秋田
kn-aut-mei=悦示
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=2
article-no=
start-page=371
end-page=377
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the influence of the general anesthesia upon the vital protecting function Part III. On the influence of various general anesthesia upon the sterilizing power of serum
kn-title=全身麻酔の生体防禦機能に及ぼす影響に関する研究 第3編 各種全身麻醉の血清殺菌力に及ぼす影響に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1) The basal narcosis caused no change of the serum sterilizing power. 2) In the intravenous anesthesia using penthothal sodium the sterilizing power showed a temporal decline but after the end of anesthesia it slightly increased. 3) In the endotracheal anesthsia with cyclopropaine, the sterilizing power always showed a marked decline. 4) In the endotracheal anesthsia with ether, the sterilizing power showed a temporal decline during and after anesthesia. 5) In the endotracheal anesthesia using cyclopropaine and nitrous oxide or with additional ether together, the serum sterilizing power varied as well as in that using cyclopropaine only, but did not decline so distinctly. 6) The variation of sterilizing power in the endotracheal anesthesia using nitrous oxide and ether together showed a very gradual decline and then also gradually recovered. 7) The variation of the sterilizing power caused by oxygen inhalation showed clear increment with the beginn of inhalation namely the oxygen inhalation is very important for protecting sterilizng power of serum from inhibition of it. 8) The influence of various general anesthesia upon the serum sterilizing power was independent from tne Opsonin rate in the blood coagulating time and the number of platelets.
en-copyright=
kn-copyright=

en-aut-name=YumiyamaShinobu
en-aut-sei=Yumiyama
en-aut-mei=Shinobu
kn-aut-name=弓山忍
kn-aut-sei=弓山
kn-aut-mei=忍
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=2
article-no=
start-page=359
end-page=369
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the influence of the general anesthesia upon the vital protecting function Part II. On the influence of various general anesthesia upon the Opsonin rate in the blood
kn-title=全身麻醉の生体防禦機能に及ぼす影響に関する研究 第2編 各種全身麻醉の血中オプソニン率に及ぼす影響に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The rise and fall of the Opsonine rate in the blood during various general anesthesia was investigated. 1) The basal narcosis scarsely gave influences upon the Opsonin rate. 2) During the intravenous anesthesia using Penthothal sodium the Opsonin rate fell to the bottom at the second hour of anesthesia. 3) During the endotracheal anesthesia with cyclopropaine the Opsonine rate showed its least value also at the second hour. 4) During the endotracheal anesthesia with ether, the Opsonin rate showed a gradule and marked increase with the progress of anesthesia. 5) During the endotracheal anesthesia using cyclopropaine and nitrous oxide together the Opsonin rate decreased markedly and after hours it showed the least value, then began to increase from the third day after anesthesia and on the 7th and 14th days, it recovered to the rate before the anesthesia. 6) During the endotracheal anesthesia using the combination of cyclopropaine, nitrous oxide and ether, the Opsonin rate decreased markedly at the first hour of the anesthesia then considerably increased at the second hour but considerably decreased at the third hour. And it gradually increased from the third day after the anesthesia. 7) During the endotracheal anesthesia using nitrous oxide and ether, it decreased markedly at the first hour of anesthesia, while it showed gradual decrease from second hour to 7th day after anesthesia. 8) By oxygen inhalation the Opsonin rate showed a slight decrease temporally, but it recovered at the end of inhalation. 9) The influence of various general anesthesia upon the Opsonin rate in blood was independend of the blood coagulating time and the number of the platelets.
en-copyright=
kn-copyright=

en-aut-name=YumiyamaShinobu
en-aut-sei=Yumiyama
en-aut-mei=Shinobu
kn-aut-name=弓山忍
kn-aut-sei=弓山
kn-aut-mei=忍
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=2
article-no=
start-page=349
end-page=357
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the influence of the general anesthesia upon the vital protecting function Part I. On the influence of various general anesthesia upon the coagulating time of arterial and venous blood and the number of bloodplatelets
kn-title=全身麻醉の生体防禦機能に及ぼす影響に関する研究 第1編 各種全身麻醉の動脈血及び静脈血血液凝固時間ならびに血小板数に及ぼす影響に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1) The basal narcosis using Ravona, Amithal and 2% Pantopon scopolamine made no variation of blood coagulating time and platelets number. 2) During the intravenous anesthesia with Penthothal sodium the blood coagulating time was delayed temporally directly after the anesthesia. Concurring with it the number of platelets decreased but soon recovered. At this time the decrease of platelets was striking especially in the venous blood. 3) During the endotracheal anesthesia with cyclopropaine the blood coagulating time was reduced one hour after the anesthesia temporally. It was then delayed with the progress of anesthesia. The blood platelets increased and decreased according with it. They were striking in the venous blood. 4) During the endotracheal anesthesia with ether the blood coagulating time showed a distinct reduce with the progress of anesthesia and even after the end of the anesthesia its recovery was delayed. The blood platelets distinctly increased and its recovery was also distinctly delayed. These variations were characteristically more striking in the arterial blood. 5) By the anesthesia using cyclopropaine and nitrous oxide together the variation of blood coagulating time and platelets number were alike to that during the anesthesia with cyclopropaine, but they were very variable after the anesthesia. 6) The platelets variation in all kind of anesthesia was parallel with that of blood coagulating.
en-copyright=
kn-copyright=

en-aut-name=YumiyamaShinobu
en-aut-sei=Yumiyama
en-aut-mei=Shinobu
kn-aut-name=弓山忍
kn-aut-sei=弓山
kn-aut-mei=忍
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=12-2
article-no=
start-page=8419
end-page=8428
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Studies on Aspirin Allergy Part 3. Localized Skin Allergy induced by Aspirin-protein
kn-title=アスピリンアレルギーに関する実験的研究 第3編 アスピリン蛋白に依る局所性皮膚過敏性に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=There are already many reports in various allergic reactions by aspirin, but most of them deal with the reactions induced by aspirin alone or a physical compound of aspirin and serum protein, as the antigens. For this reason they failed to demonstrate the antigen-antibody reaction in vitro although they recognized the establishment of active allergy. In addition, there are few reports in which conjugates of aspirin to serum proteins are used as antigen, but there seems to be no report on any experimental localized passive allergy. In the present experiment, the author selectively sensitized rabbits and guinea-pigs using conjugates of aspirin to proteins or aspirin solutions as the antigens, and studied the localized skin allergy in these test animals. The results are as follows. 1. In the rabbits and guinea-pigs sensitized with aspirin-protein, active and passive Arthus' reactions have been recognized. 2. In the above-mentioned active and passive Arthus' reactions, a cross reaction has been recognized to occur by heterogous antigen just as in the case of the precipitation reaction. 3. In the sensitization of guinea-pigs using aspirin alone, it has been recognized that there occurs the active Arthus' reaction by aspirin-proteins and by aspirin. 4. In the active Arthus' reaction induced by the aspirin-protein, there can be recognized some reaction differences between the sensitizing antigen and the cross-antigen. The grade of these reactions is more marked in the former. 5. In the active Arthus' reaction induced in the guinea-pigs sensitized with aspirin alone, no reaction differences such as mentiones above can be recognized. 6. In the passive Arthus' reaction there can't be recognized some reaction differences between the corresponding antigen and the chemical cross antigen. 7. The animals sensitized either actively or passively with aspirin have been found to respond to sodium salicylate.
en-copyright=
kn-copyright=

en-aut-name=KimuraSusumu
en-aut-sei=Kimura
en-aut-mei=Susumu
kn-aut-name=木村晋
kn-aut-sei=木村
kn-aut-mei=晋
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=12-1
article-no=
start-page=7897
end-page=7908
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591120
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Electrolytes in Blood and Skin in Skin Diseases Part 2. On the Varition of Electrolytes in Blood and Skin of Rabbits Having Dermatitis Caused with Croton Oil
kn-title=皮膚疾患に於ける血中並びに皮膚中電解質に関する研究 第2編 実験的家兎皮膚炎に於ける血中並びに皮膚中電解質量の変動に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Following the course of the dermatitis caused with croton oil in the back of rabbits, sodium, potassium and calcium in sera and skin were determined spectrophotometrically. 1. The quantity of sodium in sera showed a slight decrease in the initial stage of the dermatitis, showed increase in the severe stage and moreover increased in tho retrogressive stage and returned to the normal value in the recovering stage. In skin, increasing from the initial stage, showed marked increase in the severe stage and then decreased rapidly and showed nearly normal value in its retrogressive stage. 2. The quantity of potassium in sera increased slightly and showed remarkable increase in the severe stage, but, decreasing rapidly, showed almost normal value in retrogressive stage. The variation of potassium in skin showed the same tendency as sodium but, in the initial stage, increase of potassium was more remarkable than sodium. 3. Calcium in sera showed aslight increase in the initial stage, showed the lowest value in the rotrogressive stage and then gradually increased but returning to the normal value was delayed. In skin, showed a slight increase in the initial stage, showed the lowest value in the severe stage but thereafter was elevated gradlualy. 4. The value of Na/K in sera showed the lowest in the severe stage and in skin the lowest was showed in the initial stage and the both declined early and were eleavtated later. 5. K/Ca in sera showed the highest value in the retrogressive stage and, in skin, the highest was showed in the severe stage and the both were elevated in early period and dropped in the latter half. 6. Almost similar tendency of the variation of electrolytes was showed in the sera and the skin, but in latter more remarkable.
en-copyright=
kn-copyright=

en-aut-name=KuniharaKakuso
en-aut-sei=Kunihara
en-aut-mei=Kakuso
kn-aut-name=国原角三
kn-aut-sei=国原
kn-aut-mei=角三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部皮膚科泌尿器科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7409
end-page=7424
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinical Studies on the Taste Part II Studies on the Correlation Amung the Contained Eubstance in Blood, the Acidity of Gaetric Fluid, the pH of Saliva and the Taste
kn-title=味覚の臨床的研究 第2編 血液含有物質，胃液酸度及び唾液pHと味覚との関係に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The corrlation among the cotained substance in blood, the acidity of fastric fluid the pH of saliva and the taste was observe on the attacked paiod and convalescen pariod of disease. And the resulte were as follows. 1. Observing on the correlation between the dosis of serum sodium and the throshold value of taste, the threshold value of sweed taste showed the high value in many cases with the low value of serum sodium at the attacked period of disease and it showed the declining tendency with the increase of serum sodium by the recovery of disease. No fixed correlation between the dosis of serum sodium a d the salt, sour or bitter taste was observed. 2. The correlation between the dosis of serum chloride and the threshold value of saste was similar to that on the occasion of serum sodium. 3. As for the correlation between the dosis of serum potassium and the threshold value of taste, many cases with the high value of serum potassium at the attacked period of disease showed the high value of serum potassium at the attacked period of disease showed the high threshold value of sweet and bitter taste and they showed the returning tendency to normal value at the convalescent perio with normal value of serum potassioum. No fixed correlation between the dosis of serum potassium and the threshold value of salt or sour taste was observed. 4. As for the correlation bet ween the dosis of serum iron and the threshold value of taste, the threshold value of bitter taste showed the tendency taking the high value on the low value of serum iron. The tendency was especially remerkable in the patients with anemia. No fixed relation between the dosis of serum iron and the threshold value of sweet, salt or sour taste was not observed. 5. As for the correlation between the dosis of serum copper and the threshold value of taste, the theshold value of sweet and sour taste showed the tendency to declins, as the dosis of serum copper became decreased at the attaked period of disease in many cases. And they showed the tendency to decline with the decrease of serum copper at the convalescent period, but no fixed correlation between the desis of rerum copper and the threshold value of salt or bitter taste. 6. The correlation between the increase or decrease of blood sugar at the hunger time and the rise of decline of the threshold value of sweet or sour taste was observed on various diseases, other than diabetes, In diabetes, the threshold value of sweet taste at the hunger time showeed the high valun, but no fixed correlation between the blood sugar at the hunger time and the threshold value of salt or bitter taste was observed. 7. No fixed correlation among the dosis of serum protein, the acidity of gastric fluid, the pH of saliva the threshold value of taste was observed.
en-copyright=
kn-copyright=

en-aut-name=HondaKimiaki
en-aut-sei=Honda
en-aut-mei=Kimiaki
kn-aut-name=本田公昶
kn-aut-sei=本田
kn-aut-mei=公昶
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7291
end-page=7304
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Electrolytes in Blood and Skin in Skin Diseases Part 1. On the Variation of Electrolytes in Blood in Various Skin Diseases
kn-title=皮膚疾患に於ける血中並びに皮膚中電解質に関する研究 第1編 各種皮膚疾患に於ける血中電解質量の変動に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to explain the physiological conditions in skin diseases by searching the tendency of metabolism of electrolytes, the contents of sodium, potassium, calcium and chlor in serum were measured in 122 cases of various skin diseases. (1) Comparatively many cases of the numerous skin diseases showed the variation of the contents of electrolytes, but it was not always remarkable and constantly regular. (2) In bullous diseases, acquired pigmentary disturbances, diffuse scleroderma and erythroderma, varied the most remarkably, and especially in the 2 former cases was pointed out the evident tendency. (3) In eczema, dermatitis, inflammatory keratosis, erythematodes and cutaneous tuberculosis were often found abnormal values, but very irregular and complicated. (4) In urticaria, drug eruption and erythema exsudativum multiforme showed slight changes. (5) The auther conceived that the metabolism of electrolytes in vivo was so influenced by the several organs, especially endocrine organs, and by the autonomous nervous system, that such irregularity of this variation was showed.
en-copyright=
kn-copyright=

en-aut-name=KuniharaKakuzo
en-aut-sei=Kunihara
en-aut-mei=Kakuzo
kn-aut-name=国原角三
kn-aut-sei=国原
kn-aut-mei=角三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部皮膚科泌尿器科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7267
end-page=7278
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Supplement of Bromsulphalein Test Part II Studies on the Influence of Blood pH. Organic Matter and Inorganic Salt Upon the Excretion of Bromsulphalein into Bile
kn-title=Bromsulphalein試験に関する知見補遺 第2編 B. S. Pの胆汁中排泄に及ぼす血液pH，有機物，無機塩類の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1. The higher the pH of serum was, the more was the serum bromsulphalein value and the bromsulphalein value excreted into bile was decreased. 2. There was no special correlation between the serum pH and colloidal reaction. 3. The remarkable changes of bromsulphalein dosis excreting into bile and of serum bromsulphalein value were not observed on the increase of urea dosis in blood, in the canine with the fistula of gallbladder. 4. The serum bromsulphalein value became a little high on the increase of lactic acid dosis in blood, in the same canine, and the bromsulphalein dosis excreted into bile was considerably decreased. 5. The serum bromsulphalein value became a little high on the increase of uric acid dosis in blood, in the same canine, and the bromsulphalein dosis excreted into bile was considerably decreased. 6. The special changes of serum bromsulphalein value and bromsulphalein dosis excreating into bile were not observed on the increase of sodium in blood, in the same canine. 7. The serum bromsulphalein value became decreased on the increase of potassium in blood, in the same canine, and the bromsulphalein dosis excreted into bile was increased. 8. The serum bromsulphalein value became a little high on the increase of calcium in blood, in the same canine, but the remarkable change of bromsulphalein excreted into bile was not observed.
en-copyright=
kn-copyright=

en-aut-name=IwamiSeishin
en-aut-sei=Iwami
en-aut-mei=Seishin
kn-aut-name=岩見整親
kn-aut-sei=岩見
kn-aut-mei=整親
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7253
end-page=7265
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Supplement of Bromsulphalein Test Part I Studies on the Combination of Bromsulphalein and Serum Protein
kn-title=Bromsulphalein試験に関する知見補遺 第1編 Bromsulphaleinと血清蛋白質との結合に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1. The absorption maxima at 593, 590 and 588 mμ were observed on the coloration by the N/100 caustic soda solution at the pH 7.5 after the addition of bromsuplhalein into human serum albumin, blood plasma and serum. 2. The combined rate of bromsulphalein with albumin was conversely increased with the decrease of bromsulphalein density on the combination of human serum protein and bromsulphalein by the utilization of paper electrophoresis and the rate of wondering pigment was gradually decreased, but the rate of residual scanty bromsulphalein combined with globulin or attached in the filter paper was almost fixed. 3. Observing the influence to the combination of serum albumin and bromsulphasein on the change of various buffer used for the paper electrophoresis, the combination was obstructed in order of veronal soda, hydrochloric acid, primary potassium phosphate, secondary natrium phosphate, borax, boric acid and veronal soda buffer. 4. Observing the influence to the combination of serum albumin and bromsulphalein on the change of pH after the addtion of buffer into normal human and dilutied serum with distilled water, the combination of them were rapidly dcreased around the pH 8-9 and it became almost zero around the pH 12. 5. In the previous experiment, the combination was more decreased with the addition of urea, lactic acid or uric acid in each than without their addition, but it showed the fixed combination by the kind of buffer on the addition of urea. the extremely scanty decline of combination on the addition of lactic acid and the considerably remarkable decline of combination. 6. Sodium chloride gave no influence of the combination of albumin and bromsulphalein in the serum of same pH, potassium chloride acted to increase the combination, and calcium chloride acted to bromsulphalein itself and acted to control the coloration by caustic soda.
en-copyright=
kn-copyright=

en-aut-name=IwamiSeishin
en-aut-sei=Iwami
en-aut-mei=Seishin
kn-aut-name=岩見整親
kn-aut-sei=岩見
kn-aut-mei=整親
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7163
end-page=7169
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Combination of Bilirubin with Proteins Part 2. A Study on the Combination of Bilirubin with Globin
kn-title=Bilirubinと蛋白との結合に関する研究 第2編 Birirubinとglobinとの結合に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to study the combination of bilirubin with globin and also the combination of bilirubin with globin+albumin, the author estimated the absorption curves by means of paper electrophoresis using veronal buffer solution at pH 8.5 as the electrolyte and by paper chromatography at the first dimension using a 2% cane sugar solution as a developer, as well as by D-K type Beckman's autospectrophotometer; and obtained the follo wing results. 1. Sodium bilirubinate that is a direct bilirubin and dibasic acid bilirubin, an indirect bilirubin, both haye the capacity to combine with with globin. 2. Such combinations of bilirubins and globin do not control the mode of bilirubin to the diazo reaction. 3. When sodium bilirubinate is dissolved in the mixed solution of globin and albumin, it will combine with either one, namely, with globin and with albumin. Moreover, in this instance the mode of the proteins just as in the mode of sodium bilirubinate, shows a direct response to the diazo reaction but never an indirec response.
en-copyright=
kn-copyright=

en-aut-name=OhtsukiMasanosuke
en-aut-sei=Ohtsuki
en-aut-mei=Masanosuke
kn-aut-name=大月昌之助
kn-aut-sei=大月
kn-aut-mei=昌之助
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=11-1
article-no=
start-page=7155
end-page=7161
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19591020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Combination of Bilirubin with Proteins Part 1. Combination of Bilirubin with Albumin and with Globulin
kn-title=Bilirubinと蛋白との結合に関する研究 第1編 Bilirubinとalbumin及びglobulinとの結合について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to study the combinations of bilirubin with albumin and with globulin using a veromal buffer solution (pH 8.5) as the elctrolyte the author estimated the absorption curve by means of paper electrophoresis and by paper chromatography at the first dimesnion using 2% cane sugar solution as the developer and with D-K type Beckman's autospectro-photometer; and obtained the following results. 1. Sodium bilirubinate that is a direct bilirubin and dibasic acid bilirubin which is an indirect bilirubin both combine with albumin. 2. The mode how bilirubin combines with albumin can not be the controlling factor of the direct or the indirect bilirubin reaction. 3. When desalting is done on the combined solution of sodium bilirubinate and albumin, a mixed solution of bilirubin and albumin is obtained, but no bilirubin can be isolated. 4. It is quite difficult to combine blirubin with globulin.
en-copyright=
kn-copyright=

en-aut-name=OhtsukiMasanosuke
en-aut-sei=Ohtsuki
en-aut-mei=Masanosuke
kn-aut-name=大月昌之助
kn-aut-sei=大月
kn-aut-mei=昌之助
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=10-2
article-no=
start-page=6953
end-page=6965
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Quantitative Determination of Urobilin Body Part 1 Studies on the Quantitative Methods of Urobilin Body
kn-title=Urobilin体の定量に関する研究 第1篇 Urobilin体定量法の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The Terwen's reduction method and the methods reported by Heilmeyer, Krebs and Watson were fully observed. And the results were as follows. 1. Urobilin was completely reduced to urobilinogen within 2 hours by the Terwen's method, and the highest uroblinogen value was observed on the mixed rate of the materials and the reducing agents in 40:5:5. The materials should be stored, under the isolation of air and light, at the low temperature as possible and there was a limitation for the preserved time. 2. The optimal range of pH for the extraction of urobilinogen into the solvont. 3. It was demonstrated that petroleum ether was the best solvent for the extraction of urobilinogen since the comparative studies on the various solvent, especially between ether and petroleum ether. 4. The composition of regent needed the concentration of 0.3% P-dimethylaminobenzaldehyde on the use of 60% hydrochloric acid and of 1% P-dimethylaminobenzaldehyde on the use of 50% hydrochloric acid. 5. The shaking process for one minute was needed till the addition of sodium acetate after the addition of regents, urobilinogen in the solvent was extracted into hydrochloric acid in the regent and the reaction was performed in the water layer by the above treatment. The progress of aldehyde reaction on this occasion was considerably quantitative. 6. The significance of adding sodium acetate was to shift to nearly the pH 4 after the terminal reaction and was to remove the coloration by indole body, with bringing the coloration of urobilinogen up to the highest and keeping the stability of it. 7. The influence of indole, indican, P.A.S. and sulfonamid derivatives shown the similar reaction in the qualitative test could be eliminated on the quantitive method.
en-copyright=
kn-copyright=

en-aut-name=WatanabeHaruo
en-aut-sei=Watanabe
en-aut-mei=Haruo
kn-aut-name=渡辺春生
kn-aut-sei=渡辺
kn-aut-mei=春生
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=10-1
article-no=
start-page=6587
end-page=6594
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Production of Bile Pigment in vivo Part 2. A Spectrochemical Study on the decomposition Processes of Oxyhemoglobin and Carboxyhemoglobin in the Presence of Ascorbic acid and Molecular Oxygens
kn-title=試験内胆汁色素生成に関する研究 第2編 Oxyhaemoglobin及びCarboxyhaemoglobinの1-Ascorbin酸と分子酸素による分解過程に関する分光化学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By studying spectrochemically the decomposition processes of carohemoglobin, oxyhemoglobin, methemoglobin, and carboxyemoglobin in the presence of 1-ascorbic acid and molecular oxygens the author obtained the following results. 1. In the case of carbohemoglobin besides tha already-known absorption peaks of 556 mμ and 430 mμ it shows another absoorption peak at 760 mμ, and its EmM is 1.4-2.0. 2. The absorption curve of the 670 mμ and 630 mμ substances at the time of deoxydation by sodium dithionite and causatic soda show the absorption peak at 618 mμ and 760 mμ. 3. The decomposition of caroxyhemoglobin takes similar decomposition processes as taken by oxyhemoglobin and methemoglobin, producing the 670 mμ and 630 mμ substances. In other words, it seems that caroxyhemoglobin is reduced to hemoglobin by liberating carbon monoxide and then it enters into decomposition processes, and its decomposition is extremely slow as compared with that of oxyhemoglobin and methemoglobin. 4. CO-choleglobin is not an intermediate product of carboxyhemoglobin decomposition but it is produced at the time when choleglobin comes in contact with carbon monoxide. 5. CO-choleglobin is split into carbon monoxide and choleglobin in the presence of 1-ascorbic acid and molecular oxygens.
en-copyright=
kn-copyright=

en-aut-name=TarumiShoji
en-aut-sei=Tarumi
en-aut-mei=Shoji
kn-aut-name=垂水昭二
kn-aut-sei=垂水
kn-aut-mei=昭二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=10-1
article-no=
start-page=6577
end-page=6585
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Production of Bile Pigment in vivo Part 1. Spectrochemical Study on Decomposition Processes of Methemaglobin by 1-Ascorbic Acid and Molecular Oxygens
kn-title=試験内胆汁色素生成に関する研究 第1編 Methaemoglobinのl-Ascorbin酸と分子酸素による分解過程に関する分光化学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By amking 1-ascorbic acid and molecular oxygens act upon methemoglobin the author studied the decomposition processes spectrochemically and obtained the following results. 1. In preparing methemoglobin from oxyhemoglobin with sodium nitrite no spectrochemical effect of sodium nitrite can be recognized on methemoglobin. 2. Methemoglobin is reduced to oxyhemoglobin in the presence of 1-ascorbic acid and molecular oxygen and thereafter it shows a similar decomposition processes as observable in the case of oxyhemoglobin. 3. This reaction is affected by molecular oxygens namely, when the quantity of molecular oxygen is large, the speed of the reaction is greater but the production of 670 mμ substance is inhibited; whereas when the quantity of molecualr oxygen is small, the absorption index of 670 mμ and 630 mμ substances is greater. 4. The 670 mμ and 630 mμ substances that are intermediate substances of this reaction are reversible to one another depending upon the quantity of moecular oxygen present. 5. This reaction is affected by the ion concentration; namely, between pH 6.8 and pH 7.7 at pH 7.2 the 670 mμ and 630 mμ substances are produced in the greatest amounts. 6. This reaction is affected by 1-ascorbic acid; namely, with the increase in the amount of ascorbia acid the speed of reaction is accelerated. 7. This reaction is affected also by temperature; namely, with a rise in temperature the reaction is accelerated whereas with a fall in temperature the reaction is retarded. 8. At an eary stage of this reaction dipyrromethen serlies substances are produced by the decomposition of the 670 mμ and 630 mμ substances.
en-copyright=
kn-copyright=

en-aut-name=TarumiShoji
en-aut-sei=Tarumi
en-aut-mei=Shoji
kn-aut-name=垂水昭二
kn-aut-sei=垂水
kn-aut-mei=昭二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=10-1
article-no=
start-page=6485
end-page=6490
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Tuberculin Reaction Part 1. on the Changes of Tuberculin Antigen due to Digestive and chemical and Chemical Degeneration
kn-title=ツベルクリン反応に関する研究 第1編 消化並びに化学的変性に依るツベルクリン抗原性の変化について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By making protein degenerant and protein digestive enzyme act on old tuberculin the author performed the tuberculin reaction, and obtained the following results. 1. In loading a strong potassium iodide solution to old tuberculin the tuberculin reaction grows weaker along with an increase in the concentration of potassium iodide. This suggests that free tyrodine radical in the old tuberculin protein is involved in the tuberculin reaction. 2. In loading sodium nitrite to old tuberculin the tuberculin reaction likewise grows weaker with increase in the concentration of sodium nitrite 3. In the addition of formalin to old tuberculin the tuberculin raction is weakened along with increaes in the concentration of formalin solution. 4. When crystalline trypsine is made to act on old tuberculin, the tuberculin reaction is weakened along with lapse in the digestion time. 5. When pepsin is made to act on old tuberculin, the tuberculin reaction is likewise weakened along with lapse in the digestion time. 6. From these results it is deduced that the tuberculin reastion is mainly controlled by tuberculin protein.
en-copyright=
kn-copyright=

en-aut-name=MishimaShiro
en-aut-sei=Mishima
en-aut-mei=Shiro
kn-aut-name=三島四郎
kn-aut-sei=三島
kn-aut-mei=四郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=9-2
article-no=
start-page=6173
end-page=6186
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590910
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Histamine Release from Intracellular Particles of Dog's Liver
kn-title=犬肝臓の細胞内顆粒からのin vitro Histamine遊離に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In vitro histamine release from intracellular large granules of the dog liver was determined and compared with that from the chopped tissue. The histamine release from the large granules depended on the concentration, duration of action, temperature and pH of histamine liberators. Histamine was released by all the liberators tested even at 0°C less than at 37°C. The action of basic liberators such as sinomenine increased with increasing alkalinity of medium.
Dibucaine hydrochloride, decylamine hydrochloride, HgCl(2), quinine hydrochloride and tutocaine hydrochloride at pH 7-8 and 6mM concentration released over 50 per cent of histamine from the large granules. Under the same conditions ethylmorphine hydrochloride, toluidine blue, procaine hydrochloride, saponin (0.1 per cent), tropacocaine hydrochloride, sodium cholate, Compound 48/80 (0.1 per cent), sinomenine hydrochloride, Tween 20 (0.2 per cent), cocaine hydrochloride and xylocaine hydrochloride revealed the histamine liberating action in the descending order named. Histamine release from the granules by these substances was larger in variable degrees as compared with that from the chopped tissue. Sodium salicylate inhibited the release from the granules and chopped liver by other substances, while diphenhydamine and guaiazulen did not reveal such an inhibitory action but rather accelerated the release though slightly. The histamine release from the chopped tissue by sinomenine and decylamine was inhibited by uranil nitrate, but it was not the same from the large granules. The lack of oxygen accelerated the histamine release by decylamine from both the granules and chopped tissues, but did not reveal any significant effect on the action of other liberators. In in vitro anaphylaxis histamine release occurred in the chopped tissue but not in the granules. As far as basic liberators are concerned, there seemed to be some correlation between the histamine release ability and the heparin combining power. However, the surface activity or the hemolytic power of liberators and the histamine release ability was not to be necessarily in parallel with each other in degree. Interpreting the intracellular large granules as the granules of mast cells and in the light of the above findings the author discussed the respective mode of action of the liberators on mast cells and on their granules.
en-copyright=
kn-copyright=

en-aut-name=JinzenjiKei
en-aut-sei=Jinzenji
en-aut-mei=Kei
kn-aut-name=秦泉寺圭
kn-aut-sei=秦泉寺
kn-aut-mei=圭
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部薬理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=9-2
article-no=
start-page=5921
end-page=5928
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590910
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Histochemical Studies on Retinal Degeneration Treated with Sodium Iodate Report III.) On the Changes of Lipids and Mitochondria
kn-title=沃度酸曹達による網膜変性症の組織化学的研究 第3報 脂質並びにミトコンドリアの変化に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author studied on the changes of the lipid and mitochondria in experimental degeneration of the retina of the rabbit caused by intravenous injection of 5% solution of sodium iodate.
Lipids in the pigment epithelinm slightly decrease with the exception of those in the lipochrin. No remakable change was observed in the lipids of the outer segments and ellipsoid of vesual cells. Most of the mitochondria in pigment epithelial cells disappeared as the cells degenerate, but some of them remained unchanged even in rather advanced stages of degeneration.
en-copyright=
kn-copyright=

en-aut-name=KoyamaYasuta
en-aut-sei=Koyama
en-aut-mei=Yasuta
kn-aut-name=小山泰太
kn-aut-sei=小山
kn-aut-mei=泰太
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部眼科学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=9-1
article-no=
start-page=5695
end-page=5704
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590830
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Histochemical Studies on the Degeneration of Retina Treated with Sodium Iodate Report II. On Histopathological Changes and HAMAZAKI'S Hg-Keto Enol Substance
kn-title=沃度酸曹達による網膜変性症の組織化学的研究 第2報 クロームケトエノール物質並びにD. N. A. の変化に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author studied on the early histochemical changes of the peculiar retinal degeneration of the rabbit treated with intravenous injection of 5% solution of sodium iodate as the preceding report. The Cr-K E S (chrmo-keto-enol substance of HAMAZAKI), chief constituents of which are nucleotides of D N A, in the pigment epithelium and outer segments of rods begins to diminish gradually after 12 hrs. D N A decreases as the pigment epithelium and visual cells degenerate. In general, the Cr-K E S diminishes and disappears earlier than the Hg-K E S in the pigment epithelium and in visual cells. The authers that sodium iodate acts rather indirectly on the metabolism of the D N A and related substances in the outer layers of the retina.
en-copyright=
kn-copyright=

en-aut-name=KoyamaYasuta
en-aut-sei=Koyama
en-aut-mei=Yasuta
kn-aut-name=小山泰太
kn-aut-sei=小山
kn-aut-mei=泰太
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部眼科学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-2
article-no=
start-page=5363
end-page=5374
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590815
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Changes of the Electrolyte in the Cerebral Cortex of Dogs during Convulsions
kn-title=痙攣時における犬大脳皮質の各種電解質の変動に関する実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Changes of the electrolyte in the motor area of the cerebral cortex were experimentally studied in dogs during convulsions which were caused by the intravenous injection of metrazol. The results were as follows: 1) Chloride content increases at the preconvulsive stadium and also at the acme and reaches to its maximum at the repeated convulsive stadium. At the postconvulsive stadium it decreases nearly to the normal, but still remains a little higher than the normal 2) Sodium content decreases at the preconvulsive stadium and recovers gradually to the normal as the time elapses. 3) Potassium content shows no distinguished change. 4) Calcium content reaches the highest at the preconvulsive stadium and then decreases gradually to the normal as the time elapses. 5) Magnesium content at the preconvulsive stadium is the same as the normal and increases rapidly at the acme and at the repeated convulsive stadium but finally decreases below the normal. 6) From these facts stated above, it is considered that the changes of the electrolyte in the cerebral tissue during the convulsion are caused by the secondary changes due to convulsion.
en-copyright=
kn-copyright=

en-aut-name=YorimaeHiroshi
en-aut-sei=Yorimae
en-aut-mei=Hiroshi
kn-aut-name=頼前博
kn-aut-sei=頼前
kn-aut-mei=博
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=8-1
article-no=
start-page=4613
end-page=4627
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Adrenal Cortical Function in Hypoplastic Anemia Part 3. The Function of Adrenal Cortex of Patients with this Disease
kn-title=再生不良性貧血における副腎皮質機能に関する研究 第3編 患者の副腎皮質機能
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With a view to clarify the cause of hypoplastic anemia the author studied the adrenal cortical function in patients with this disease, and obtained the following results. 1. The basic metabolic rates show not any definite inclinations. 2. In the insulin tolerance test, the insulin index has been found to have declined. 3. As for electrolytes in serum, chlorine is decreased but phosphorus is increased to a high degree, and ratio Na/K is slightly high while calcium, sodium and potassium show no definite inclinations. 4. Robinson-Power-Kepler water test is almost positive. 5. On measuring 17-KS and free chemocortioids (Ch. C.) excreted in the urine, the amount of 17-KS is decreased conspicuously but Ch. C. is increased. 6. In the gel-ACTH loading test the adrenal cortical reserve function has been found to have declined highly. 7. From these facts stated above, it is possible to assume that there is a decline in the adrenal cortical function in this disease.
en-copyright=
kn-copyright=

en-aut-name=KurozumiJiro
en-aut-sei=Kurozumi
en-aut-mei=Jiro
kn-aut-name=黒住治郎
kn-aut-sei=黒住
kn-aut-mei=治郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=6-2
article-no=
start-page=3601
end-page=3608
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Histochemical and Biochemical Studies on the Respiratory Enzymes Part 1. The Reduction Mechanism of Tetrazolium Salts by the Respiratory Enzyme System
kn-title=呼吸酵素系の組織化学的並に生化学的研究 第1編 呼吸酵素系によるテトラゾリウム塩の還元機構について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the purpose to establish the integrating methods for the selective histochemical demonstration and colorimetric estimation of each enzyme and enzyme system belonging to the terminal respiratory enzyme system, an alytical study on the reduction of neotetrazolium chloride by tissue homogenates was carried out in the present report using various substrates and inhibitory agents, and the steps with which the reaction of neotetrazolium chloride reduction conjugates were determined as follows. 1. The reaction of neotetrazolium reduction by tissue homogenetes using sodium succinate as substrate is not the succinic dehydrogenase reaction but the succinoxidase system reaction; and the reaction takes place cojugating about 50 per cent in the step of the succinic dehydrogenase system, of these about 15 per cent conjugates in the step prior to the antimycin A sensitive step and 35 per cent in the step itself; and about 50 per cent in the step of cytochrome c oxidase. 2. In the case using p-phenylenediamine as substrate the reaction of neotetrazolium reduction is the reaction due to the activity of cytochrome c-cytochrome oxidase system, and when p-phenylenediamine is used with the sufficient amount of cytochrome c, the reaction appears to be dependent on cytochrome c oxidase activity. Neotetrazolium reduction in all these reactions takes place conjugating in the step of cytochrome c oxidase. 3. In the case where DPN and substrates taking DPN as a coenzyme are used, the reaction of neotetrazolium reduction is mainly the reaction conjugating at the step below antimycin A sensitive step in the DPNH-cytochrome c reductase system. 4. Endogenous dehydrogenase reactions are the sum total reactions conjugating at the steps prior to the antimycin A sensitive step in the terminal electron transport system and with other various reduction systems which are not inhibited by antimycin A.
en-copyright=
kn-copyright=

en-aut-name=OkazakiHiroaki
en-aut-sei=Okazaki
en-aut-mei=Hiroaki
kn-aut-name=岡崎博明
kn-aut-sei=岡崎
kn-aut-mei=博明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部病理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=6-2
article-no=
start-page=3403
end-page=3418
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Absorptions in the Granuloma Pouch and Artificial Connective Tissue Pouch
kn-title=肉芽嚢及び人工結合織嚢の吸収能に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=As a step to investigate the pathological physiology of joints, the author attemped to study the absorption of sodium salicylate and carbon black in granuloma pouch (Selye) of rats and artificial connective tissue pouch (Terazawa) of rabbits and obtained the following results: 1. The absorption of sodium salicylate in the granuloma pouch of rats and in the artificial connective tissue pouch of rabbits is very rapid. 2. The absorption of 20%-polyvinyl pyrrolidon-sodium salicylate is slower than in the former. 3. In the pouch of the hydrocortisone-protected rat (Selye), the proliferation of the granuloma tissue and carbon absorption in the granuloma pouch are inhibited. 4. When the daily injection of 100mg/kg sodium salicylate is administered intraperitoneally to rats, the proliferation of granuloma tissue and the carbon absorption are slightly inhibited. 5. After the injection of hydrocortisone into the rat granuloma pouch, necrosis can be observed on the surface of the pouch wall, proving that there is a little carbon absorption. 6. Although the carbon absorption in the pouch having remarkable fibrosis is retarded, it can be accelerated by the scraping or washing of the pouch wall.
en-copyright=
kn-copyright=

en-aut-name=ShigemasaMotoyuki
en-aut-sei=Shigemasa
en-aut-mei=Motoyuki
kn-aut-name=重政素行
kn-aut-sei=重政
kn-aut-mei=素行
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部整形外科
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=6-2
article-no=
start-page=3357
end-page=3369
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Study on Electrolytes Changes in Hypothermia and Effects of Operation on Its Metabolism Part II. Experimental Study of Operative Effects on Electrolytes Changes in Hypothermia
kn-title=低体温麻酔および低温下手術における電解質の変動に関する実験的研究 第2編 低温下手術における電解質の変動に関する実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effects of operation on seruml evel and urinary excretion of sodium, chloride and potassium, and water and electrolytes coutent in tissue were studied on dogs in both hypothermic and normothermic conditions. Following results were obtained. 1) Hyponatremia and hypochloremia, decrease of urinary excretion of both electrolytes, increase of potassium-sodium ratio and increase of urinary excretion of potassium in accordance with decrease of urine volume were found during postoperative period in dogs who underwent operation under normothermia. However, increase of urine volume occurred on about 5th or 6th postoperative day resulting in increase of urinary excretion of sodium and chloride, and decrease of potassium excretion. Increase of water, sodium and chloride content of the tissue which had operative intervention, were found on the 4th postoperative day, though decrease of potassium was noted. 2) Decrease of serum sodium and chloride, and increase of potassium were noticed in hypothermic group, though these changes were not so significant as in nomothermic group. Urinary excretion of sodium and chloride increased in accordance with increase of urine volume and chloride-sodium ratio were diminished. However, changes after 5th to 6th postoperative day were same in both groups. There were found no significant changes in water and electrolytes content of tissue, which had operative intervention, on the 4th postoperative day in hypothermic group. Increase of water, sodium, and chloride in tissue were slight and no decrease of potassium was found. It was anticipated based on these findings that excretion of ADH and function of adrenal gland were suppressed resulting in increase of urinary excretion of sodium and chloride with less retention of water, sodium and chloride in tissue during postoperative period in operation performed under hypothermia. In addition to, decrease of potassium content in tissue was not found, suggesting of less consumption of body protein and suppression of catabolism during postoperaetive period.
en-copyright=
kn-copyright=

en-aut-name=MizushimaMasatoshi
en-aut-sei=Mizushima
en-aut-mei=Masatoshi
kn-aut-name=水島正俊
kn-aut-sei=水島
kn-aut-mei=正俊
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=6-2
article-no=
start-page=3345
end-page=3356
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Study on Electrolytes Changes in Hypothermia and Effects of Operation on Its Metabolism Part I. Experimental Study on Electrolytes Changes in Hypotherm
kn-title=低体温麻酔および低温下手術における電解質の変動に関する実験的研究 第1編 低体温麻酔における電解質の変動に関する実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Changes in serum and urine electrolytes, sodium and potassium in erythrocytes, water and electrolytes contents in tissue were studied in dogs under hypothermia. Following results were obtained. 1) Changes in serum sodium and calcium are not found, though potassium decreases and chloride increases in hypothermia. 2) No marked changes in sodium and potassium in erythrocytes are found. 3) Urinary excretion of sodium and chloride decrease, while potassium increases. No changes in excretion of calcium are noted. Total amount of electrolytes excreted in urine decrease because urine volume is markedly diminished in hypothermia below 28°C. 4) No significant changes in water content in tissue are found. There is found no changes in sodium of tissue. Chloride in tissue decreases, while potassium in liver increases though no changes are found in other organs. Calcium in tissue remains constant.
Decrease of serum potassium and increase of chloride are remarkable changes in electrolytes in hypothermia. It is anticipated that deposition of potassium develops in liver because of decrease of glucose utilization due to low metabolism and decreased consumption of glycogen with resulting glycogen formation from excessive glucose which carries potassium from the extracellular fluid into the cells. Increase of serum chloride is supposed to be due to mobilzation of intracellular chloride into blood and decrease of excretion in urine.
en-copyright=
kn-copyright=

en-aut-name=MizushimaMasatoshi
en-aut-sei=Mizushima
en-aut-mei=Masatoshi
kn-aut-name=水島正俊
kn-aut-sei=水島
kn-aut-mei=正俊
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=6-2
article-no=
start-page=3289
end-page=3299
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590515
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Urinary Excretion of Histamine in the Rat Part 2. Actions on the Urinary Excretion of Histamine, of the Histamine Releasers of Different Classes and of the Substances Affecting Histamine Release
kn-title=ラットにおけるHistamineの尿中排泄に関する研究 第2報 Histamineの尿中排泄に対する諸種Histamine遊離物質及びHistamine遊離に影響を及ぼす物質の作用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With the administration of histamine there occurs a transient increase of the urinary excretion of free histamine in the female rat being loaded with water and whose urine being collected every 30 minutes. In the previous paper (Folia pharmacol. japon. 54, 1221, 1958) it was reported that such an increase is dependent upon the basal rate (a) of the urinary excretion of histamine and the amount (b) of histamine excessively excreted after intraperitoneal injection of a limited amount of histamine and that there is a relationship of b/a=k (k stands for constant pertaining dose). On the basis of these findings and taking the increase in the urinary excretion of histamine as an index, the author compared the potency and time course of in vivo histamine release as demonstrated by sinomenine hydrochloride, Compound 48/80, quinine hydrochoride, sodium cholate, dextran, egg white, decylamine and Tween 20, administered intraperitoneally. Judging from the relationship between the degree of the increase in the urinary excretion of histamine and the amount of the substances being administered, the histamine-releasing ability of these substances was found to be in the order of; Compound 48/80>decylamine>sinomenine>sodium cholate>quinine, Tween 20>dextran and egg white. Of them, Compound 48/80 and sinomenine showed the increase in the urinary histamine exactly identical with that observed after histamine administration, taking the shortest time course; while in the cases of quinine, sodium cholate, dextran, and egg white the increase was somewhat slower; and by decylamine and Tween 20 it developed most slowly yet most persistently.
In the rats previously treated with guaiazulene, cortisone, aminopyrine or cinchophen sodium, increases in the urinary excretion of histamine by all the releasers mentioned above were similarly inhibited. Since mechanisms of histamine release action are not the same by different releasers, it seems that the action of these inhibitors is of such a nature as to be manifested at a common stage involved in different patterns of the mechanism of histamine release. Such an action of cortisone was more marked in a relatively small dose rather than in a large dose, suggesting presence of an adequate amount of this steroid to be used for the manifestation of this action. The histamine-releasing ability of egg white was inhibited in the alloxan-diabetic rat, while on the contrary, it was greatly accelerated in the insulin-treated rat. Such an effect of alloxan was completely antagonized by an adequate amount of insulin. However, in the rat with glycosuria induced by glucose administration, sensitivity to egg white was not altered. Succinic acid or oxaloacetic acid given with the purpose to inhibit the formation of keton bodies, could not eliminate the above mentioned alloxan effect. Both alloxan and insulin did not in any way modify the histamine-releasing effect of sinomenine.
en-copyright=
kn-copyright=

en-aut-name=KondoKazuji
en-aut-sei=Kondo
en-aut-mei=Kazuji
kn-aut-name=近藤和二
kn-aut-sei=近藤
kn-aut-mei=和二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部薬理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4-1
article-no=
start-page=1237
end-page=1251
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Function of the Pituitary Adrenocortical System in Idiopathic Hypochromic Anemia Part 3. A Study on the Tissue Metabolism through Screening Test
kn-title=本態性萎黄貧血の下垂体副腎皮質系に関する研究 第3編 Screening Testによる物質代謝について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With a view to clarify the cause of idiopathic hypochromic anemia the author studied the function of the adrenocortical system, this time, by observing the changes in the tissue metabolism through the screening test. 1. The basal metabolic rate showed a slight increase and it was mcstly increased in males while it was mostly at the normal level in females. 2. As for serum inorganic substances chlorine was decreased but sodium and phosphate were increased; and the ratio Na/K was high, showing irregular tendency in the function of the pituitary adrenocortical system involving the mineral metabolism. 3. By Robinson-Power-Kepler water test 29.4per cent showed positive rate, indicating a slight fall in the function of the pituitary adrenocortical system involved in hydroelectrolytic metabolism. 4. In the insulin tolerance test the insulin tolerance index and a decline in insulin resista nce and recovery of hypoglycemia were found to have decreased slightly. However, by treatment the insulin resistance improved but the recovery of hypoglycemia rather became worse.
en-copyright=
kn-copyright=

en-aut-name=YamamotoShomi
en-aut-sei=Yamamoto
en-aut-mei=Shomi
kn-aut-name=山本昭美
kn-aut-sei=山本
kn-aut-mei=昭美
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4-1
article-no=
start-page=1165
end-page=1180
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Relationship Between Function of Autonomic Nervous System and Pentothal induced intravenos Anaesthesia Part III. The Investigation of the distribution of Pentothal in the Body influenced by cholinesterase activity and Blood Pressure
kn-title=Pentothal・Sodium静脈麻酔時間と自律神経機能に関する実験的研究 第3編 自律神経剤によるChE活性値および循環動態の変動がPentothal・Sodium体内分布に及ぼす影響に関する実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1) The increased cholinesterase activity coursed by administration of Acetylcholine, pilocarpine, Imidalin and chloromazine reduce the concentration of pentothel in the Brain and Serum. 2) The decreased cholinesterase actriity coursed by administration of physostigmin, prostigmin and DFP increase the concentration of pentothal in the Brain and Serum. 3) The mutation of distribution of pentothal in the Body coursed by administration of Ephedrin, Epinephrin and Norepinephrin should be attributable to both increased Blood pressure and reduced cholinesterase activity. 4) The mutation of anaesthetic duration and the distribution of pentothal in the body changed by administration of various drugs of autenomic nervous system should be attributable to both cholinesterase activity and Blood pressure influenced by these drugs.
en-copyright=
kn-copyright=

en-aut-name=MatsudaYoshio
en-aut-sei=Matsuda
en-aut-mei=Yoshio
kn-aut-name=松田義朗
kn-aut-sei=松田
kn-aut-mei=義朗
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4-1
article-no=
start-page=1151
end-page=1164
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Relationship Between Function of Autonomic Nervous System and Pentothal induced intravenos Anaesthesia Part II. The Investigation of the distribution of Pentothal in the Body changed by administration of Varions drugs of Autonomic nevous system
kn-title=Pentothal・Sodium静脈麻酔時間と自律神経機能に関する実験的研究 第2編 自律神経毒剤加のPentothal・Sodium体内分布に及ぼす影響に関する実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Following the estimation of anaesthetic duration, I investigated the distribution of pento thal in the body (Brain, Blood, Kidney, Leber, Muscle. Fat), taking advantage of Beck-mantype Spectroptomoter. 1) Epinephrin, Norepinephrin, Ephedrin coursed the increased coucentration of pentothal in the Brain, Kidney, Muscle and Fat, and coursed the decreased concentration in the blood. 2) The prolonged duration of anaestetic coursed by administration of prostigmin, physostigmin, D. P. E. are shown to be correlateed with an increased rate of penetration of anaesthetic in the blood and brain. 3) Pyruvate also prolonged the duration of anaesthetic and this prolongation is reduced by addited administration of acetylcholin. These above results show that the inhibition of cholinacetylase by pyruvate coursed the increased permeability to pentothal and that addition of acetylcholin reverse the effect.
en-copyright=
kn-copyright=

en-aut-name=MatsudaYoshio
en-aut-sei=Matsuda
en-aut-mei=Yoshio
kn-aut-name=松田義朗
kn-aut-sei=松田
kn-aut-mei=義朗
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1（陣内）外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=659
end-page=672
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Expression and purification of the HIV-1 env gene products in Escherichia coli
kn-title=HIV-1エンベロープ蛋白質の大腸菌での発現と精製
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=To purify the HIV-1 envelope protein with antigenic reactivity, the Pvu II-Bgl II fragment of the HIV-1 env gene, from the Pvu II site to the second Bgl II site, encoding the carboxyl terminal 180 amino acids of the viral surface protein (SU, gp120) was molecularly cloned in Escherichia coli strain HB101 using protein A expression-shuttle vector pRIT5. The pRIT5 contains the protein A gene, encoding the secretion signal and IgG binding domain of protein A with the upstream promoter and the downstream multicloning sites, as well as the two replication sites for Escherichia coli and Staphylococcus aureus. A fused protein with the molecular weight of about 55 kilodaltons was produced, which showed the same reactivity as the native protein A against rabbit serum IgG on Western blotting analysis. Most of the fused protein in the periplasmic space was degraded, while the complete fused protein inside the cells was recovered as an insoluble protein. The fused protein was solubilized with sodium dodecylsulfate (SDS), partially purified by IgG sepharose affinity chromatography, and completely purified by SDS-polyacrylamide gel electrophoresis. The quantity of the expressed fused protein was estimated about 1% of the total proteins. The purified fused protein contained 516 amino acids with Mr54, 976, consisting of 305 amino acids of the IgG binding domain of protein A, 5 amino acids derived from polylinker, a carboxyl terminal 180 amino acids of the HIV-1 envelope surface protein gp120, and 26 amino acids derived from the pUC19 sequence.
en-copyright=
kn-copyright=

en-aut-name=ZhangBo
en-aut-sei=Zhang
en-aut-mei=Bo
kn-aut-name=張波
kn-aut-sei=張
kn-aut-mei=波
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌源研究施設生化学部門
en-keyword=HIV-1
kn-keyword=HIV-1
en-keyword=エンベロープ蛋白質
kn-keyword=エンベロープ蛋白質
en-keyword=発現ベクター
kn-keyword=発現ベクター
en-keyword=Protein A
kn-keyword=Protein A
en-keyword=IgG-Sepharoseカラム
kn-keyword=IgG-Sepharoseカラム
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=11-12
article-no=
start-page=1253
end-page=1259
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1990
dt-pub=199012
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Usefulness of avirulent strains in investigation of latent infection of herpes simplex virus type 1
kn-title=単純ヘルペスウイルス1型潜伏感染の研究における弱毒株の有用性
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The usefulness of avirulent strains of herpes simplex virus type 1 (HSV-1) in investigations of latency was examined. Trigeminal ganglia (TG) latently infected with HSV-1 were obtained more efficiently from mice inoculated with an avirulent strain, SKO-1B, than from those inoculated with a virulent strain, F. Moreover, utilization of two avirulent strains, SKO-1B and -GCr Miyama, allowed us to compare the rate of reactivatable latency (RL) in these strains. Cultivation of explants of TG recovered from mice inoculated with the same dose of either infectious virus revealed a significant difference in the rate of RL between the two strains. Both strains were revealed to be neither hypersensitive nor resistant to acyclovir, indicating that the difference in the rate of RL between the two strains was not due to any deficient activity of viral ribonucleotide reductase and/or thymidine kinase. The recovery rate of the -GCr virus from explants of TG latently infected with this virus was greatly enhanced by treatment of the explants with sodium n-butyrate during in vitro cultivation. As such, at least one of the mechanisms responsible for causing a very low rate of RL in TG infected with -GCr Miyama may be reduced expression of immediate-early genes of this virus in latently infected cells in vitro.
en-copyright=
kn-copyright=

en-aut-name=AraoYujiro
en-aut-sei=Arao
en-aut-mei=Yujiro
kn-aut-name=荒尾雄二郎
kn-aut-sei=荒尾
kn-aut-mei=雄二郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部ウイルス学教室
en-keyword=herpes simplex virus type 1
kn-keyword=herpes simplex virus type 1
en-keyword=latent infection
kn-keyword=latent infection
en-keyword=reactivation
kn-keyword=reactivation
en-keyword=neurovirulence
kn-keyword=neurovirulence
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=7-8
article-no=
start-page=843
end-page=849
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1990
dt-pub=199008
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A case of primary aldosteronism with severe hypokalemic myopathy
kn-title=著明な低カリウム血性ミオパチーをきたした原発性アルドステロン症の一例
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We report a rare case of primary aldosteronism with severe hypokalemic myopathy in a 59-year-old female who had suffered from hypertension since 1979. Because of severe muscle weakness, especially of extremities, she became unable to rise from her bed and was admitted to the Kitagawa hospital on July 10, 1989. Symptoms such as tetany, polyuria, and diarrhea were not observed. Her consciousness was clear. The blood pressure was in the range of 152 mmHg systolic and 104 mmHg diastolic. Systolic heart murmur (Levine II), cardiac arrythmia, and slight edema on the legs were noticed. She did not have any sign of muscular atrophy. Serum aldosteron level was 220 pg/ml, and plasma renin concentration was less than 0.5 ng/ml/h. The serum level of potassium was 1.7 mEq/l, sodium 150 mEq/l, GOT 47 IU/l, LDH 507 IU/l, CPK 850 IU/l (MM type 99%), and aldolase 9.2 mIU/ml. Computed tomography (CT) after intravenous contrast injection revealed a round low density mass (1.6 cm in diameter) in her left adrenal gland. CT also revealed the gallbladder adenomyomatosis. Her left adrenal gland and gallbladder were surgically removed on July 26, 1989. After the operation, the blood pressure and laboratory data including serum potassium returned to normal, and she became able to pursue her ordinary life. Endoscopy revealed a gastric polyp which was hyperplastic. Histopathologically the removed adrenal gland showed an adrenocortical adenoma consisted of clear cells and nodular hyperplasia of the glomerular zone.
en-copyright=
kn-copyright=

en-aut-name=OhtaniYuko
en-aut-sei=Ohtani
en-aut-mei=Yuko
kn-aut-name=大谷裕子
kn-aut-sei=大谷
kn-aut-mei=裕子
aut-affil-num=1
ORCID=

en-aut-name=YoshinoTadashi
en-aut-sei=Yoshino
en-aut-mei=Tadashi
kn-aut-name=吉野正
kn-aut-sei=吉野
kn-aut-mei=正
aut-affil-num=2
ORCID=

en-aut-name=AkagiTadaatsu
en-aut-sei=Akagi
en-aut-mei=Tadaatsu
kn-aut-name=赤木忠厚
kn-aut-sei=赤木
kn-aut-mei=忠厚
aut-affil-num=3
ORCID=

en-aut-name=KitagawaTakashi
en-aut-sei=Kitagawa
en-aut-mei=Takashi
kn-aut-name=北川堯之
kn-aut-sei=北川
kn-aut-mei=堯之
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二病理学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部第二病理学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部第二病理学教室

affil-num=4
en-affil=
kn-affil=北川病院
en-keyword=低カリウム血性ミオパチー
kn-keyword=低カリウム血性ミオパチー
en-keyword=原発性アルドステロン症
kn-keyword=原発性アルドステロン症
en-keyword=二次性高血圧症
kn-keyword=二次性高血圧症
en-keyword=副腎皮質腺腫
kn-keyword=副腎皮質腺腫
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=
article-no=
start-page=34
end-page=41
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=195309
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=STUDIES OF THE RADIOACTIVE SPRINGS (XXXIII) EFFECT OF RADIOACTIVE THERMAL BATH ON POTASSIUM, CALCIUM, SODIUM, CHLORIDE AND PROTEIN LEVELS IN SERUM
kn-title=放射能泉に関する研究（XXXIII）放射能泉入浴の血中鉱質に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to study the effect of radioactive thermal bath on mineral substances in blood, the author bathed rabbits in Misasa Hot Spring (Yamada-Yu) once daily for 5 minutes dnring the successive 3 weeks, and determined potassium, calcium, sodium, chloride and protein levels
in serum before and after the bath every week. The radon content of Yamada-Yu was 300-600×10(-10) curies per liter, its water temperature being 42-44°C. at that time. In the early stage of serial baths calcium decreased, and potassium, sodium, chloride and protein content increased. But in the later stage a reversed tendency was recognized. It was concluded that radioactive thermal baths of Misasa had no peculiar effect on mineral substances in rabbit's serum, compared with the effects of the other kinds of thermal baths
en-copyright=
kn-copyright=

en-aut-name=OndaSakue
en-aut-sei=Onda
en-aut-mei=Sakue
kn-aut-name=音田作衛
kn-aut-sei=音田
kn-aut-mei=作衛
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学溫泉研究所内科
END

start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=
article-no=
start-page=1
end-page=13
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=195306
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=STUDIES ON THE AGING OF MINERAL WATERS (I) CHANGES IN THE RADON CONTENT OF THERMAL WATERS OF MISASA AFTER FLOWING OUT
kn-title=溫泉の老化に関する研究（第1報）三朝溫泉の湧出後のラドン含量の変化
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author determined the radon content of the thermal waters of Misasa, Tottori Prefecture Japan, at various times after flowing out, and obtained the following results: 1) The velocity of variation is expressed by a simple equation with respect to the radon content. At thermal temperaturee of 41.5-41.8°C and room temperature of 24.3-28.0°C, a following exrerimental equation was obtained, u:;ing a beaker with a inner diameter of about 17 cm and a height of 27 cm, log a = -0,0048t + 2.59 a: radon content in Mache unit. t : time in minute. 2) A close relation was observed between the room temperature and the rate of variation of radon, and also between the thermal temperature and the rate of variation of radon, that is, the rate of variaton of radon was proportional to the room temperature and the thermal temperature respectivly, as it is clearly seen from the distribution coefficient of radon. 3) There exists a c1ose relation between the velocity of variation of radon and the surface area being in contact with air, that is, the velocity was generally proportional to the square root of the surface area, or to the radius of the surface. 4) The velocity of variation of radon content was roughly inversely proportional to relative humidity of air. 5) Betweeen the range of sodium chloride concentrations from 0.1 to 40.1 g. per liter no difference was proved concerning the velocities of variation in radon content.
en-copyright=
kn-copyright=

en-aut-name=SugiharaTakeshi
en-aut-sei=Sugihara
en-aut-mei=Takeshi
kn-aut-name=杉原健
kn-aut-sei=杉原
kn-aut-mei=健
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学溫泉研究所化学部
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=
article-no=
start-page=54
end-page=63
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=195301
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A CLINICAL, STUDY ON HEARTBURN
kn-title=胸やけに就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=402 consecutive patients with various disorders of digestive tract were investigated concerning heartburn and 184 cases (45%) proved to have heartburn. No significant relationship was established between the heartburn and gastric acidity or the kinds of digestive diseases. Heartburn was not only seen in hyperacidity but also often seen in hyp-and anacidity or normacidity. No significant correlation was observed between the heartburn and the rate of emptying the gastric content. Flow back of bile in stomach juice was relatively often seen in cholecystopathic patients with heartburn, but no such relationship was proved in other kinds of patients. Experimentally beartburn was evoked in the patients with heartburn by administration of 30 cc of 1% sodium bicarbonate solution in the stomach or ten cc in oesophagus, but not by the same quantity of hydrochloric acid (N/20), lactic acid (N/20), 10% syrup, 50% polytamine solution, physiological saline, or 5% bile dilution. A habit of eating fast was observed relatively often in heartburn patient. Profession of the most of the above examined patients was farmer and they noted some causative relation to large intake of food rich in carbohydrate (sweetpotato, rice-cake, etc. ). The most frequent time for heartburn to beginn was one to three hours after a meal.
en-copyright=
kn-copyright=

en-aut-name=SotozonoMasazumi
en-aut-sei=Sotozono
en-aut-mei=Masazumi
kn-aut-name=外園正純
kn-aut-sei=外園
kn-aut-mei=正純
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所内科
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=
article-no=
start-page=6
end-page=10
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=195309
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=STUDIES ON THE AGING OF THE MINERAL WATERS (Ill) VARIATION OF SILICATE IN THEMINERAL WATERS
kn-title=温泉の老化に関する研究（第3報）　溫泉水中の珪酸塩の溶存狀態
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=When silicate content is determined by the colorimetric method, the silicate content in the mineral waters and sodium silcate solution decreases in addition of ammonium hydroxide and sodium carbonate respectively, and moreover after addition of aluminium ion in the samples, when aluminium hydroxide is precipitated from ammonium hydroxide solution, total silicates in various forms are coprecipitated with it. The silicate content in the mineral waters that are kept in concentrations of 0.1～0.4 normality of sodium hydroxide showed special variations with times.
en-copyright=
kn-copyright=

en-aut-name=SugiharaTakeshi
en-aut-sei=Sugihara
en-aut-mei=Takeshi
kn-aut-name=杉原健
kn-aut-sei=杉原
kn-aut-mei=健
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所化学部
END

start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=
article-no=
start-page=18
end-page=23
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=195312
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=BALNEOLOGICAL STUDIES USING RADIOACTIVE ISOTOPES (3)
kn-title=人工放射性同位元素による温泉作用の研究 (3)　浴水中の硫酸イオンの体内進入に及ぼす連続浴，火傷治癒経過，色素塗布並びに硫酸カルシウム水溶液の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=By using labelled sodium sulfate or calcium sulfate (sulfate containing S(35)) the transition of sulfate ion into the body across the skin was investigated soon after taking a bath in sodium
sulfate or calcium sulfate solution under varing conditions. Mice were used for experiments. The percutaneous absorption of sulfate ion proved to decrease gradually in the course of serial baths in sodium sulfate solntion and was accelerated by making a fresh burn on the skin of the bathed animals, but it showed a tendency to decrease as the wound became more and more healed. The application of basic dye to the skin (e.g. 1% methylenblue or 1% fuchsin solution) increased the transition of sulfate ion into the test animal. Little difference was proved between the bath in sodium sulfate and calcium sulfate solution with respect to the penetration of sulfate ion. Passage of sulfate ion from the bath water through the skin was a little promoted by the use of sodium sulfate solution than by the use of calcium sulfate solution.
en-copyright=
kn-copyright=

en-aut-name=YokotaTakeo
en-aut-sei=Yokota
en-aut-mei=Takeo
kn-aut-name=横田剛男
kn-aut-sei=横田
kn-aut-mei=剛男
aut-affil-num=1
ORCID=

en-aut-name=AshizawaTakashi
en-aut-sei=Ashizawa
en-aut-mei=Takashi
kn-aut-name=芦沢峻
kn-aut-sei=芦沢
kn-aut-mei=峻
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所内科

affil-num=2
en-affil=
kn-affil=岡山大学温泉研究所
END

start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=
article-no=
start-page=5
end-page=8
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=195312
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ON THE EXISTENCE OF RADIUM B, RADIUM C AND THORIUM B IN MISASA HOT SPRINGS
kn-title=鳥取県三朝温泉に於ける二，三の放射性元素の存在について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author tried to measure RaB, RaC and ThB in the hot spring waters using G-M counter and proved the existence of RaB and ThB in Misasa Hot Springs. To 100 liters of spring water ahout 20 gms of ferric chloride and then sodium hydroxide were added. Precipitate was dissolved in hydrochloric acid and after adding bismuth and lead, the solution was saturated with hydrogen sulfide. The precipitate of sulfides was filtered and ignited to ashes after drying. The β-activity of the ashes was measured by a mica-window type G-M counter with the recording circuits of scale of 16. Natural background was 50±5 connts per minutes. Six spring waters of Misasa, namely "Spring of Branch Laboratory", "Nakayu", "Gunze" "O-T-R", "Jwayu", and "Tsukiminoyu" were investigated. RaB was detected in all samples. ThB was found only in "Gunze" and "O-T-R". ThB content of "Gunze" was estimated, to be roughly 1×10(-12) Curie units per liter.
en-copyright=
kn-copyright=

en-aut-name=SatoMitsuo
en-aut-sei=Sato
en-aut-mei=Mitsuo
kn-aut-name=佐藤三雄
kn-aut-sei=佐藤
kn-aut-mei=三雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学理学部物理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=
article-no=
start-page=1
end-page=14
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1954
dt-pub=195403
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=RADON CONTENT OF HOT SPRINGS IN TOTTORI PREFECTURE, JAPAN
kn-title=鳥取県下の温泉のラドン含有量に就て
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1. Radon content of 166 thermal waters in IWAI, TOTTORI YOSHIOKA, HAMAMURA, TOGO, SEKIGANE, MISASA, and KAlKE Hot Springs was measured by I. M. Fontactoscope in the years 1950-1951. Of which 66 samples showed a radon content over 30×10(-10) curie units per liter. Namely, 50 springs in Misasa, 6 in Sekigane, 9 in Hamamura, and one in Togo belonged to the radioactive spring in the definition by Ministry of Social Welfare. The highest Radon content (1150×10(10) curie units per liter) was recorded in Hisuino-Yu in Misasa, where five springs had a radon content over 360×10(-10) curie units per liter. 2. No marked difference was proved between the results obtained this time and the data in the former reports concerning the radon content of these thermal springs. 3. Radon content proved higher in the springs which issue from granite than in
the springs of other districts. No definite relation was proved between the radon content and water temperature. The radon content was generally high in simple thermals or in weak sodium chloride springs, low in sulfated springs and in saline springs which had a comparatively
high sulfate content.
en-copyright=
kn-copyright=

en-aut-name=OshimaYoshio
en-aut-sei=Oshima
en-aut-mei=Yoshio
kn-aut-name=大島良雄
kn-aut-sei=大島
kn-aut-mei=良雄
aut-affil-num=1
ORCID=

en-aut-name=YamadaNaoharu
en-aut-sei=Yamada
en-aut-mei=Naoharu
kn-aut-name=山田尚春
kn-aut-sei=山田
kn-aut-mei=尚春
aut-affil-num=2
ORCID=

en-aut-name=MifuneMasaaki
en-aut-sei=Mifune
en-aut-mei=Masaaki
kn-aut-name=御船政明
kn-aut-sei=御船
kn-aut-mei=政明
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=2
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=3
en-affil=
kn-affil=岡山大学温泉研究所
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=
article-no=
start-page=37
end-page=54
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=195807
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Paper-Analysis in the Field of Balneology (I) Studies on Iron in Serum by Means of Parper-Electrophoresis and Paper-Chromatography
kn-title=温泉医学領域に於ける濾紙分析法の研究　（1） 濾紙電気泳動法並びに濾紙chromatographyによる血清鉄の研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1. The author showed by means of paper-electrophoresis using o-nitrosoresorcinmonomethylether (hereafter N. R. M. E.), a colourdeveloping reagent, that iron in serum combines with β-fraction of serum protein. 2. The author proposed a new method for the determination of serum iron by means of paper-chromatography. Procedure is as follows. In a centriguged tube, 0.5 ml. of clear serum is taken, acidified with 0.15 ml. of 20% HNO(3), and allowed to stand for 20 minutes. After addition of 0.2 ml. of saturated solution of CH(3)COONa and 1 ml. of acetic acid-sodium acetate buffer solution (pH: 3.8), the mixture is kept for at least 10 min. and filtered through Toyo-filter-paper No.5 A. The clear filtrate is taken in a stoppered test tube (the inside diameter: 1.2 cm.; height: 15 cm.). To this filtrate are added 0.2 ml. of 20% Hydroxylamine hydrochloride and 0.25 ml. of saturated aqueous solution of N. R. M. E. After being allowed to stand for at least 20 min., the mixture is shaken with 5 ml. of carbon tebrachloride. Then the excess of N. R. M. E. is removed. The supernatant aqueous solution (0.2 ml.) is used for a determination sample. The sample is placed on the paper (Toyo,filter-paper No.51 or 50) in a thin line at a distance of 10 cm. from the edge, and the spots are developed with 90 % ethylalcohol. After development for 4-6 hours, a green-coloured linear spot appsars at a distance of 12-16 cm. from the origin-point. Spot intensity at 670 mμ is measured with Natsume's densitometer, and from this, concentration of Fe can be estimat.ed on the standard graph (Fig. 5). 3, Effects of radioactive thermal spring bathing and internal use of vitriol water upon serum iron levels were studied with the result.s as follows. The above-mentioned author's method of determining the iron level in a small amount of serum is very convenient in investigating iron metabolism in the field of balneotherapy. The author examined changes of iron levels in serum after radioactive thermal bathing as well as after internal use of acid vitriol water. a) The iron levels in serum were maasurecl by the author's method before and 5, 30 and 60 minutes after the radioactive thermal bath (Rn-content: 10-30 Mache, 42-3°C., for 10 min,). The iron levels in the serum of healthy subjects showed no significant change after the thermal bathing. b) Yanahara Mineral Water (an acid vitriol water, pH; 2.2) contains 0.045 gm. of Cl(-), 12.35 gm. of SO(4)(--), 2.5 gm. of Fe(++)+Fe(+++), 0.0938 gm. of Al(+++) and 0.0001 gm. of Cobalt in one liter. i) Thirty ml. of Yanahara, water diluted with plain water to 200 ml. (Fe(++) content: ca. 75 mg.) and administered to health fasting subjects orally. As a control matter, 0.65 gm. of Glukon-F powder (Fe(++) content.: ca. 75 mg.) was then given. Blood samples were taken from the cubital vein before and 1, 3 and 6 hours after the intake of the dtriol water. Iron contents of the serum samples were measured by the author's paper chromatographic method. The results were shown in the table and the figure. The iron contents of the serum after the intake of the mineral water were higher than in the case of the control. ii) Two ml. of Yanahara vitriol water was administered to rabbits with the aid of a stomach tube, and serum iron levels wera determined before and 1, 3, 4 and 6 hours after the administration. An amount of 0.05 gm. of Glukon-F powder was dissolved in 5 ml. of plain water, and this solution was given to rabbits as a control. After the internal use of vitriol water, iron levels in serum rose and remained unexpactedly high for about 1-4 hours, in a manner that did not. correspond to the amount of iron in the given mineral water. So that it was suspected that the iron originally contained in the living subjects had been mobilized by the intake of vitriol water. Iron levels in serum after administration of a solution of Glukon-F powder were lower than in the case of the mineral water.
en-copyright=
kn-copyright=

en-aut-name=IshibashiMaruo
en-aut-sei=Ishibashi
en-aut-mei=Maruo
kn-aut-name=石橋丸応
kn-aut-sei=石橋
kn-aut-mei=丸応
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所
END

start-ver=1.4
cd-journal=joma
no-vol=23
cd-vols=
no-issue=
article-no=
start-page=1
end-page=22
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=195810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Study of Togo -Matsuzaki Hot Springs, Tottori Prefecture
kn-title=鳥取県東郷松崎温泉に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1. Layers containing thermal water in this district are thin, and lie at different depths (about 35, 55, and 60 meters) from the ground surface. There are evidences to show that these layers are intimately connected with one another. 2. The authors may suppose the existence of a structurally weak zone, along the line from Matsuzaki to Asozu, within which the issuing spots of thermal springs are located. 3. The head water levels of the thermal springs in this district are closely related with that of Lake Togo. Keeping pace with the variations of the water levels of Lake Togo and of artesian wells in its vicinity, the rate of flow of thermal springs vary; and the correlation between these variations is apparent. 4. The pumping suction of thermal water at one spring affects the flow of
water at other springs within distances of 150 to 200 meters therefrom, though the direct sources of thermal water supply for the latter springs may be different from that of the former. 5. The spring water in this district is considered to be a mixture of hot water, containing sodium, calcium, chloride, and sulfate ions, and cold water, containing bicarbonate ion. The diversity of chemical constitutions of different spring waters is explained as due to the difference in proportion in which the hot and cold waters
are mixed.
en-copyright=
kn-copyright=

en-aut-name=UmemotoShunji
en-aut-sei=Umemoto
en-aut-mei=Shunji
kn-aut-name=梅本春次
kn-aut-sei=梅本
kn-aut-mei=春次
aut-affil-num=1
ORCID=

en-aut-name=HaradaMitsuru
en-aut-sei=Harada
en-aut-mei=Mitsuru
kn-aut-name=原田光
kn-aut-sei=原田
kn-aut-mei=光
aut-affil-num=2
ORCID=

en-aut-name=OkabeShigeru
en-aut-sei=Okabe
en-aut-mei=Shigeru
kn-aut-name=岡部茂
kn-aut-sei=岡部
kn-aut-mei=茂
aut-affil-num=3
ORCID=

en-aut-name=MiyakoshiJunichiro
en-aut-sei=Miyakoshi
en-aut-mei=Junichiro
kn-aut-name=宮腰潤一郎
kn-aut-sei=宮腰
kn-aut-mei=潤一郎
aut-affil-num=4
ORCID=

en-aut-name=SakanoueMasanobu
en-aut-sei=Sakanoue
en-aut-mei=Masanobu
kn-aut-name=阪上正信
kn-aut-sei=阪上
kn-aut-mei=正信
aut-affil-num=5
ORCID=

en-aut-name=TanakaMasaya
en-aut-sei=Tanaka
en-aut-mei=Masaya
kn-aut-name=田中昌也
kn-aut-sei=田中
kn-aut-mei=昌也
aut-affil-num=6
ORCID=

en-aut-name=Mifune Masaaki
en-aut-sei=Mifune Masaaki
en-aut-mei=
kn-aut-name=御船政明
kn-aut-sei=御船
kn-aut-mei=政明
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=2
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=3
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=4
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=5
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=6
en-affil=
kn-affil=岡山大学温泉研究所

affil-num=7
en-affil=
kn-affil=岡山大学温泉研究所
END

start-ver=1.4
cd-journal=joma
no-vol=41
cd-vols=
no-issue=
article-no=
start-page=35
end-page=48
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1972
dt-pub=19720325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on chrysotherapy in rheumatoid arthritis, III. Gold determination by the atomic absorption spectrophotometry
kn-title=慢性関節リウマチの金療法に関する研究 III 原子吸光分光分析法による検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The gold concentration in serum, synovial fluid and urine has been determined using a HITACHI MODEL 207 atomic absorption spectrophotometer. Flow rate of acetylene was setted at 2.0l/min. and lamp current was setted at 8mA. The samples were pretreated by the determination of gold. Serum was diluted with deionized water by two to ten-fold. To O.2ml. of synoival fluid 50units/ml of hyaluronidase was added and then incubated for 20 minutes at 37℃. This sample was then treated the procedure for serum given previously. Urine was treated using the modification method of Christions procedure. The recovery rates with gold sodium thiomalate in serum, synovial fluid and urine were 99.9%, 102.5% and 93.6% respectively. When gold sodium thiomalate was injected in rabbits, the maximum serum gold level was attained 1 hour after injection and then gradually decreased at about 50% of the maximum level 24 hours after injection. In the observations of changes in the gold levels in the serum of patients with rheumatoid arthritis after injection of gold sodium thiomalate and gold thioglucose, its peak level after gold thioglucose injection tended to be slightly lower as compared with the same amount of gold sodium thiomalate injection. In those patients who had been given 300 mg. or more of gold salt-preparation, one additional injection of 25 mg. of gold salt could attain the gold level of around 200μg/100ml, in serum a week later, and this level was very nearly the same with that after 3-4 days of one additional injection of 1Omg. of gold salt. The amount of gold excreted in the urine varied from patient to patient, and the mean excretion rates of gold sodium thiomalate and gold thioglucose in the urine within 24 hours after intramuscular administralion were 9.8% and 6.5% respectively. The simple, rapid and accurate method for analysis in biological specimens using atomic absorption spectrophotometer would offer one of the excellent measures in the clinical management of patients with rheumatoid arthritis.
en-copyright=
kn-copyright=

en-aut-name=TakahashiKazue
en-aut-sei=Takahashi
en-aut-mei=Kazue
kn-aut-name=高橋和枝
kn-aut-sei=高橋
kn-aut-mei=和枝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学温泉研究所温泉医学部門
END

start-ver=1.4
cd-journal=joma
no-vol=60
cd-vols=
no-issue=51
article-no=
start-page=11969
end-page=11975
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2004
dt-pub=200412
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Reactivity of TEMPO anion as a nucleophile and its applications for selective transformations of haloalkanes or acyl halides to aldehydes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Sodium 2,2,6,6-tetramethylpiperidine-N-oxide (TEMPO&#8722;Na+), generated by reduction of TEMPO· with sodium naphthalenide in THF, reacted with alkyl halides or acyl halides to produce O-alkylated or acylated TEMPOs, which were in turn oxidized with mCPBA or reduced with DIBAL-H to afford the corresponding aldehydes, thus accomplishing a new protocol for the halides-carbonyls conversion.</p>

en-copyright=
kn-copyright=

en-aut-name=InokuchiTsutomu
en-aut-sei=Inokuchi
en-aut-mei=Tsutomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KawafuchiHiroyuki
en-aut-sei=Kawafuchi
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Toyama National College of Technology
en-keyword=TEMPO and compounds
kn-keyword=TEMPO and compounds
en-keyword=Oxidation
kn-keyword=Oxidation
en-keyword=Reduction
kn-keyword=Reduction
en-keyword=mCPBA
kn-keyword=mCPBA
en-keyword=DIBAL-H
kn-keyword=DIBAL-H
en-keyword=Aldehyde
kn-keyword=Aldehyde
END

start-ver=1.4
cd-journal=joma
no-vol=136
cd-vols=
no-issue=9
article-no=
start-page=2606
end-page=2608
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=19881130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Studies on the acid-base properties of the AIBr3-NaBr melts
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The acid-base properties of the ZnBr[2]-NaBr melts at 623 K were investigated on the basis of the electromotive force measurements of a zinc-zinc concentration cell. The following two chemical equilibria were postulated to describe the acid-base character of the melts ZBnr[2]+Br[-]=ZnBr[3][-] K[1] ZnBr[3][-]+Br[-] =ZnBr[4][2+] K[2] The equilibrium constants K[1] and K[2], were determined to be 5.0×10, and 1.0×10[2], respectively, at 623 K</p>
en-copyright=
kn-copyright=

en-aut-name=HayashiHidetaka
en-aut-sei=Hayashi
en-aut-mei=Hidetaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HayashiN
en-aut-sei=Hayashi
en-aut-mei=N
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakeharaZ
en-aut-sei=Takehara
en-aut-mei=Z
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KatagiriA
en-aut-sei=Katagiri
en-aut-mei=A
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Kyoto University

affil-num=3
en-affil=
kn-affil=Kyoto University

affil-num=4
en-affil=
kn-affil=Kyoto University
en-keyword=liquid mixtures
kn-keyword=liquid mixtures
en-keyword=aluminium compounds
kn-keyword=aluminium compounds
en-keyword=sodium compounds
kn-keyword=sodium compounds
en-keyword=bromine compounds
kn-keyword=bromine compounds
en-keyword=chemical equilibrium
kn-keyword=chemical equilibrium
en-keyword=electrochemical analysis
kn-keyword=electrochemical analysis
en-keyword=thermodynamic properties
kn-keyword=thermodynamic properties
en-keyword=thermochemistry
kn-keyword=thermochemistry
en-keyword=electric potential
kn-keyword=electric potential
END

start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=24
article-no=
start-page=5103
end-page=5111
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2004
dt-pub=20041215
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A theoretical interpretation of the chemical shift of Si-29 NMR peaks in alkali borosilicate glasses
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In Si-29-NMR, it has so far been accepted that the chemical shifts of Q(n) Species (SiO4 units containing n bridging oxygens) were equivalent between alkali borosilicate, and boron-free alkali silicate classes. In the sodium borosilicate glasses with low sodium content. however. a contradiction was confirmed in the estimation of alkali distributions B-11 NMR suggested that Na ions were entirely distributed to berate groups to form BO4 units, whereas a -90 ppm component Was also observed in Si-29-NMR spectra, which has been attributed to Q(3) species associated with a nonbridging oxygen (NBO). Then. cluster molecular orbital calculations were performed to interpret the -90 ppm component in the borosilicate, glasses. It Was found that a silicon atom which had two tetrahedral borons (B4) as its second nearest neighbors was similar in atomic charge and Si2p energy to the Q(3) species in boron-free alkali silicates. Unequal distribution of electrons in Si-O-B4 bridging bonds was also found. where much electrons Were localized oil the Si-O bonds. It was finally concluded that the Si-O-B4 bridges with narrow bond angle were responsible for the -90 ppm Si-29 component in the borosilicate glasses. There still remained another interpretation: the Q(3) species were actually present in the glasses. and NBOs in the Q(3) species were derived from the tricluster groups. such as (O3Si)O(BO3)(2). In the classes With low sodium content. however. it was concluded that the tricluster groups were not so abundant to contribute to the -90 ppm component.
en-copyright=
kn-copyright=

en-aut-name=NanbaTokuro
en-aut-sei=Nanba
en-aut-mei=Tokuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NishimuraMitsunori
en-aut-sei=Nishimura
en-aut-mei=Mitsunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MiuraYoshinari
en-aut-sei=Miura
en-aut-mei=Yoshinari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University
en-keyword=short-range order
kn-keyword=short-range order
en-keyword=nuclear-magnetic-resonance
kn-keyword=nuclear-magnetic-resonance
en-keyword=mas-nmr
kn-keyword=mas-nmr
en-keyword=structural
kn-keyword=structural
en-keyword=groups
kn-keyword=groups
en-keyword=oxygen sites
kn-keyword=oxygen sites
en-keyword=ab-initio
kn-keyword=ab-initio
en-keyword=o-17 nmr
kn-keyword=o-17 nmr
en-keyword=b-11
kn-keyword=b-11
en-keyword=na20-b2-3-s1-2
kn-keyword=na20-b2-3-s1-2
en-keyword=spectroscopy
kn-keyword=spectroscopy
END

start-ver=1.4
cd-journal=joma
no-vol=45
cd-vols=
no-issue=1
article-no=
start-page=183
end-page=191
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=20070920
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Sorption Characteristics of Honeycomb-Type Sorption Element Composed of Organic Sorbent
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>This study deals with the sorption characteristics of a honeycomb-type sorption element composed of a new organic sorbent that was composed of the cross-linked polymer of sodium acrylate. Transient experiments in which moist air was passed into the honeycomb-type sorption element were conducted under various conditions of air velocity, temperature, relative humidity and honeycomb length. As a result, the effective mass transfer coefficient of the organic sorbent adsorbing the water vapor was non-dimensionalized as a function of Reynolds number, modified Stefan number and non-dimensional honeycomb length.</p>

en-copyright=
kn-copyright=

en-aut-name=InabaHideo
en-aut-sei=Inaba
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KidaTakahisa
en-aut-sei=Kida
en-aut-mei=Takahisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HoribeAkihiko
en-aut-sei=Horibe
en-aut-mei=Akihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KanedaMakoto
en-aut-sei=Kaneda
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Engineering Group, Engineering Division, Japan EXLAN Co., Ltd

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Engineering Group, Engineering Division, Japan EXLAN Co., Ltd
en-keyword=Air Conditioning
kn-keyword=Air Conditioning
en-keyword=Sorption
kn-keyword=Sorption
en-keyword=Organic Sorbent
kn-keyword=Organic Sorbent
en-keyword=Water Vapor
kn-keyword=Water Vapor
en-keyword=Honeycomb Shape
kn-keyword=Honeycomb Shape
en-keyword=Mass Transfer
kn-keyword=Mass Transfer
END

start-ver=1.4
cd-journal=joma
no-vol=387
cd-vols=
no-issue=6
article-no=
start-page=2057
end-page=2064
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=200703
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=S,S,S-Tris(2-ethylhexyl) phosphorotrithioate as an effective solvent mediator for a mexiletine-sensitive membrane electrode
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=S,S,S-Tris(2-ethylhexyl) phosphorotrithioate proved to be an effective solvent mediator for constructing a mexiletine-sensitive membrane electrode in combination with an ion-exchanger, sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate. Among a series of phosphorus compounds containing phosphoryl (P=O) groups, this solvent mediator showed the highest sensitivity to mexiletine in phosphate-buffered physiological saline containing 0.15 mol L-1 NaCl and 0.01 mol L-1 NaH2PO4/Na2HPO4 (pH 7.4), giving a detection limit of 2 x 10(-6) mol L-1 with a slope of 58.8 mV decade(-1). This is the best reported detection limit of any mexiletine-sensitive electrode developed to date. Owing to its high selectivity toward inorganic cations, the electrode was used to determine the level of mexiletine in saliva, the monitoring of which is quite effective for controlling the dose of this drug noninvasively. The mexiletine concentrations determined with the mexiletine-sensitive electrode compared favorably with those determined by high-performance liquid chromatography.
en-copyright=
kn-copyright=

en-aut-name=KatsuTakashi
en-aut-sei=Katsu
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsunamotoYumi
en-aut-sei=Tsunamoto
en-aut-mei=Yumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HaniokaNobumitsu
en-aut-sei=Hanioka
en-aut-mei=Nobumitsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KomagoeKeiko
en-aut-sei=Komagoe
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MasudaKazufumi
en-aut-sei=Masuda
en-aut-mei=Kazufumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NarimatsuShizuo
en-aut-sei=Narimatsu
en-aut-mei=Shizuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=4
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=5
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=6
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=Ion-selective electrode
kn-keyword=Ion-selective electrode
en-keyword=Mexiletine determination
kn-keyword=Mexiletine determination
en-keyword=S,S,S-Tris(2-ethylhexyl) phosphorotrithioate
kn-keyword=S,S,S-Tris(2-ethylhexyl) phosphorotrithioate
en-keyword=Solvent mediator
kn-keyword=Solvent mediator
en-keyword=Drug monitoring
kn-keyword=Drug monitoring
END

start-ver=1.4
cd-journal=joma
no-vol=24
cd-vols=
no-issue=1
article-no=
start-page=27
end-page=48
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1970
dt-pub=197002
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A morphologic study of protein ingestion by Ehrlich ascites tumor cells
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>For the purpose to obtain the information of the mechanism of protein uptake by the tumor cells, some cytochemical and electron microscopic observations were carried out on Ehrlich ascites tumor cells incubated with
horseradish peroxidase (basic hemoprotein, molecular weight approximately 40,000) in vitro. In the earlier periods of the incubation peroxidase was found to be adsorbed on some area of surface of the tumor cells forming a thin protein layer, where an active pseudopodia formation was observed. With the lapse of time, the protein was taken in the deep cytoplasm by the infoldings
of the cell membrane and accumulated in the cytoplasmic vesicles having limiting membrane. Concerning the accumulation of the protein into the vesicles, small tubular structures in the cytoplasm connecting the cell
surface and the vesicles, were considered to participate in the intracellular transportation of peroxidase taken up. In cold environment (2°C), the formation of pseudopodia and deep inward infoldings of the cell membrane
was inhibited and simultaneously the uptake of peroxidase stopped. Iodoacetate and sodium fluoride also effected to suppress the pseudopodia and infoldings formation moderetely, as well as uptake of peroxidase, though they did not stop completely. These facts have indicated that
horseradish peroxdase is taken up by Ehrlich ascites tuimor cells through pinocytosis which involves energy-requiring process dependent upon glycolytic metabolism of the tumor cells.</p>

en-copyright=
kn-copyright=

en-aut-name=SogabeKoiti
en-aut-sei=Sogabe
en-aut-mei=Koiti
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=24
cd-vols=
no-issue=2
article-no=
start-page=185
end-page=204
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1970
dt-pub=197004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Binding of photosensitizing dyes with some biopolymers
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>To obtain some information of the biological action of Kankohso 101dinicotinate and Kankohso 301-nicotinate, observations were made on the binding mode of these substances with protein, chondroitin sulfate and nucleic acids and the following results were obtained; 1. Kankohso 10 I-dinicotinate binds reversively with bovine serum albumin or serum r-globulin, resulting in metachromasia. By binding with proteins the absorption maximum of the dye shifts toward the long wave length side and the absorbance decreased distinctly. The data show that there are more than one kind of binding sites and the binding with bovine serum albumin is weak in acidic solution and strong in alkaline solution. 2. Kankohso 10 I-dinicotinate produces strong metachromasia with sodium chondroitin sulfate and the color of the solution changes from violet blue to reddish violet. The absorption maximum at 592 mp. decreases
without shifting its wave length ,and the shoulder appears at 555 mp. be. comes distinct peak. The strongest metachromatical changes occurs at the concentration of the chondroitinsulfate whose sulfonate radicals is equal to the molecules of Kankohso 10 I-dinicotinate. 3. Kankohso IOI-dinicotinate produces metachromasia with nucleic acid, where absorption spectrum is shifted toward long wave length and absorbance is decreased at a certain concentration. 4. Kankohso 301.nicotinate binds weakly with bovine serum albumin, the binding of which is reversible and the maximum binding number is 1.1 per molecule of albumin. Metachromasia cannot be produced by
binding. Kankohso 30I.nicotinate does not bind with bovine serum &#947;-globulin. This compund does not produce metachromasia with sodium chondroitin sulfate but produces weak metachromasia with nucleic acid, indicating some affinity to nucleic acid.</p>

en-copyright=
kn-copyright=

en-aut-name=YasuiShigeo
en-aut-sei=Yasui
en-aut-mei=Shigeo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=46
cd-vols=
no-issue=3
article-no=
start-page=169
end-page=174
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1992
dt-pub=199206
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Concentration and uptake of taurine in umbilical blood platelets.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The concentration and uptake of taurine in the umbilical and adult blood platelets were studied. Taurine was the most abundant free amino acid in both umbilical and adult blood platelets. The taurine concentration in umbilical blood platelets (2.30 pmoles/10(4) cells) was significantly lower than that of adult blood platelets (3.27 pmoles/10(4) cells) in contrast to the reverse relationship in taurine concentrations in umbilical and adult blood plasma. No other amino acid showed such significant difference in the concentrations between umbilical and adult blood platelets. Taurine uptake into umbilical blood platelets was temperature sensitive and sodium-dependent in a manner similar to that of adult blood platelets. The uptake conformed well to Hanes-plot. The Vmax of the uptake into adult blood platelets was about 3.6 times higher than that of umbilical blood platelets, but no significant difference was seen in the Km value between the two groups.</p>

en-copyright=
kn-copyright=

en-aut-name=KanemoriHirofumi
en-aut-sei=Kanemori
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=EjiriKohei
en-aut-sei=Ejiri
en-aut-mei=Kohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AkahoriShuichiro
en-aut-sei=Akahori
en-aut-mei=Shuichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KuboTakafumi
en-aut-sei=Kubo
en-aut-mei=Takafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SekibaKaoru
en-aut-sei=Sekiba
en-aut-mei=Kaoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama Univerisity

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University
en-keyword=umbilical blood
kn-keyword=umbilical blood
en-keyword=platelet
kn-keyword=platelet
en-keyword=taurine concentration
kn-keyword=taurine concentration
en-keyword=faurine uptake
kn-keyword=faurine uptake
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=3
article-no=
start-page=147
end-page=152
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=196806
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A method of column chromatographic isolation of major phospholipid components on Escherichia coli
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>For the column chromatographic isolation of individual phospholipids from the total phospholipid mixture, silicic acid, DEAE cellulose, alumina and others, have been used as adsorbent. However, it must be emphasized that silicic acid (1, 2, 3, 4) is the most useful adsorbent for the separation of the total phospholipid mixture from each other in reasonable purity. VAN DEENEN reported that pure phosphatidyl glycerol was obtained from the lipid fraction of spinach leaves after repeated chromatography on silicic
acid column (5). The phospholipid extracted from Escherichia coli B consists of abundant phosphatidyl ethanolamine (70-80 %), cardiolipin, phosphatidyl glycerol
and other minor components as described in the previous paper (6). The high percentage content of phosphatidyl ethanolamine renders it difficult to separate the phospholipids by the column chromatography. Therefore,
repeated chromatographies on the silicic acid column treated with sodium bicarbonate (7) and normal silicic acid column were employed for the isolation of the major components from the total phospholipid of E. coli B.
Stepwise elution (4) was carried out with chloroform containing increasing proportions of methanol, and the eluent was divided into several fractions according to experience with thin-layer chromatography.</p>

en-copyright=
kn-copyright=

en-aut-name=YosiokaTieko
en-aut-sei=Yosioka
en-aut-mei=Tieko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AkatsukaKazuya
en-aut-sei=Akatsuka
en-aut-mei=Kazuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamagamiAkira
en-aut-sei=Yamagami
en-aut-mei=Akira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KanemasaYasuhiro
en-aut-sei=Kanemasa
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=6
article-no=
start-page=293
end-page=317
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=196812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A light and electron microscopic study of the distribution of gold sodium thiomalate in the rheumatoid synovial membranes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The synovial membranes from 16 rheumatoid patients treated with intramuscular injections of gold sodium thiomalate were observed by light and electron microscopy with special reference to the distribution of gold particles in the tissue. 1) Light microscopic study revealed that the gold demonstrated as cytoplasmic granules by OKAMOTO'S histochemical method were contained
in the synovial lining cells and in the macrophages around lymph-follicles and blood vessels in the subsynovial layer. In the well-developed villi on the surface of rheumatoid synovial membrane, large macrophages with gold granules infiltrated into the lymphoid cell accumulation of small lymph-follicles. 2) The deposition of gold in the synovial tissue increased with the increase of the doses of gold administered. 3) Electron microscopic observation indicated that gold particles are contained in the numerous lysosomes in the Type A and intermediate lining cells. The macrophages around lymph-follicles and blood vessels also possessed a large amount of gold particles gathered in the lysosomes of these cells. 4) Macrophages containing gold particles in their long cytoplasmic
extensions were found often in a close contact with plasma cells of various differentiation stages. A direct cytoplasmic connection was observed between the two kinds of cells but an artifact could not be excluded. 5) The effect of gold salt in the treatment of RA was discussed from the immunological view point.</p>

en-copyright=
kn-copyright=

en-aut-name=InoueHajime
en-aut-sei=Inoue
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=23
cd-vols=
no-issue=1
article-no=
start-page=21
end-page=26
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1969
dt-pub=196902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of distention of the small intestine on the movements of the gallbladder and the sphincter of Oddi
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>1. Dogs anesthetized with pentobarbital sodium were mainly used and effects of the distention of the small intestine on the movements of the gall bladder and the sphincter of Oddi were investigated.
2. The distention of the small intestine (jejunum or ileum) inhibited the rhythmic contraction of the gall bladder and duodenal movements, and relaxed the tone of the sphincter of Oddi, resulting in an increase of the outflow of fluid through the orifice of the common bile duct.
3. After cutting the bilateral thoracic splanchnic nerves together with extirpation of the bilateral upper lumbar sympathetic trunks, the inhibitory response on the movements of the gall bladder and the tone of the sphincter of Oddi was completely abolished. The vagus nerve did not take part in the reflex response described above. The transection of the spinal cord at the level between Thl and Th2 produced no change in the reflex responses.
4. Fwm the results described above it may be supposed that effects of the distention of the small intestine on the movements of the gall bladder and the sphincter of Oddi are produced via the thoracic and lumbar splanchnic nerves through the reflex center which is located in the spinal
cord.</p>

en-copyright=
kn-copyright=

en-aut-name=NakayamaSosogu
en-aut-sei=Nakayama
en-aut-mei=Sosogu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=23
cd-vols=
no-issue=3
article-no=
start-page=227
end-page=235
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1969
dt-pub=196906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The competitive effect of adenosine-5'-triphosphate against the stimulating and inhibiting actions of 2,4-dinitrophenol on the mitochondrial respiration
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Effect of ATP and substrates on 2,4-dinitrophenol-induced adenosine triphcsphatase (E. C. 3.6. 1. 4.) activity and respiration of isolated rat liver mitochondria has been investigated. 1. The oxidation of sodium succinate inhibited the action of 2, 4-DNP
on the induction of adenosine triphosphatase activity in the mitochondria. 2. A moderately large amount of sodium succinate restored the suppressed mitochondrial respiration due to 2, 4-DNP.
3. Adenosine-5'-triphosphate (ATP) restored quantitatively the released and inhibited mitochondrial respiration due to 2,4-DNP, and its prior addition prevented also quantitatively the action of 2,4-DNP on the mitochondrial oxygen up-take. These ATP effects were oligomycin
sensitive, and they were considered to manifest their actions through the phosphorylation system.</p>

en-copyright=
kn-copyright=

en-aut-name=HataseO.
en-aut-sei=Hatase
en-aut-mei=O.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamamotoG.
en-aut-sei=Yamamoto
en-aut-mei=G.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OdaT.
en-aut-sei=Oda
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=23
cd-vols=
no-issue=5
article-no=
start-page=343
end-page=356
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1969
dt-pub=196910
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Ultrastructure and biochemical function of the mitochondria in respiratory-deficient mutant yeast induced by 4-nitroquinoline nitrogen oxide
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>1. A respiratory-deficient mutant strain of yeast was obtained from wild strain of Saccharomyces servisiae by treatment with 4-nitroquinoline N-oxide. Ultrastructure and function of the wild or mutant strains and the mitochondrial fractions isolated from these strains were examined by biochemical and electron microscopic analyses.
2. The frequency of the respiratory-deficient mutant strain in yeast induced with 10-6M 4-nitroquinoline N-oxide was about 40 %. 3. Respiratory-deficient mutant strain is incapable of reducing 2, 3, 5-triphenyltetrazolium chloride salt and to grow on lactate medium. In addition to this, the mutant has been found to have lost its ability to take up oxygen in sodium succinate and pyruvate. 4. 4.Nitroquinoline N-oxide in the concentration that induces a mutant of yeast cells or its kin inhibits the oxygen uptake in normal strain. 5. The normal strain of yeast is characterized by difference spectrum corresponding to cytochromes a+as, band c+Cll respectively, whereas,
the mutant strain containes almost no cytochromes a+ as, band C1 but contains normal or increased amount of cytochrome c. 6. Mitochondrial fraction isolated from mutant strain has largely lost its ability to oxidize succinate. On the other hand, NADH-, lactate-and
cytochrome c-oxidase activities are reduced by about 1/17, 1/7 and 1/8 of that of normal strain, respectively.
7. Succinate dehydrogenase activity of mutant strain is almost zero. Moreover, this activity is not affected on the addition of phenazine methosulfate. NADH dehydrogenase activity of mutant stran is about 1/2 of normal strain.
8. The variations in mitochondrial structure of normal and mutant strain in the stationary phase have been followed with the aid of electron microscopy. In contrast to the normal strain, the mutant strain revealed distinct morphological changes in mitochondria, especially, the lack of cristae in its interior. The results have been interpreted to indicate that the mutant induced by 4.nitroquinoline N.oxide has a character of cyto. plasmic mutant.</p>

en-copyright=
kn-copyright=

en-aut-name=KoshibaKimikazu
en-aut-sei=Koshiba
en-aut-mei=Kimikazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OdaTakuzo
en-aut-sei=Oda
en-aut-mei=Takuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TsukamotoHiromichi
en-aut-sei=Tsukamoto
en-aut-mei=Hiromichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=GotoNobuyuki
en-aut-sei=Goto
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=23
cd-vols=
no-issue=4
article-no=
start-page=291
end-page=302
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1969
dt-pub=196908
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=ATP synthesis of submitochondrial particles driven by proton gradient
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>l) The submitochondrial particle system can synthesize ATP in the early phase (220 seconds after the accition of ADP) in the presence of sodium succinate and Pi, in spite of the absence of the hexokinase-glucose system, and this phosphorylation is inhibited by oligomycin. 2) The submitochondrial particle system can synthesize ATP by the
base-acid transition (proton pulse) only in the presence of ADP and Pi, in spite of the absence of oxidizing substrates and the hexokinase-glucose system, and this phosphorylation is dependent on the span of pH change,
and is inhibited by oligomycin and 2, 4-dinitrophenol.
3) The role of the proton vector in the oxidative phosphorylation and the proton ejection was discussed from the stand point of a new hypothesis.</p>

en-copyright=
kn-copyright=

en-aut-name=HataseOsamu
en-aut-sei=Hatase
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=18
cd-vols=
no-issue=5
article-no=
start-page=247
end-page=259
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=196410
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of sodium oleate on the metabolism and size of rat liver mitochondria I. Uncoupling, shrinkage and their re­versal action by bovine serum albumin
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Effects of sodium oleate and bovine serum albumin (BSA) on rat liver mitochondrial function and structure were studied by measuring oxygen uptake, 90° light-scattering, adenosine triphosphatase activity and pyridine nucleotides
fluorescence. 1. The low concentration of oleate induced the uncoupling of oxidative phosphorylation and the scattering change of mitochondria. This action of oleate
differed from that of oleate at a high concentration which induces the high amplitude swelling with respect to its physiological and biochemical properties. The degrees of reversal swelling (shrinkage) and of oxygen uptake induced by oleate in the presence of Pi and succinate were altered proportionately to the concentration of oleate, and the concentration of oleate to the shrinkage coincided with that of the maximal respiratory release. 2. Antimycin A or 2, 4- dinitrophenol prevented the oleate-induced mitochondrial shrinkage, but the treatment of these agents after prior incubation with Pi and succinate allowed the shrinkage, though the degree was small in its extent compared with that in the absence of inhibitors. On the other hand, oligomycin did not affect the shrinkage with oleate. 3. BSA protected the mitochondrial phosphorylation from the uncoupling action of oleate without showing any effect of its own. A complete reversal could readily be demonstrated by a sufficient amount of BSA from the uncoupling, structural changes, and oxidation of intramitochondrial pyridine nucleotides induced by oleate in a low concentration. 4. The oleate-stimulated latent ATPase activity was proportional to the oleate-induced shrinkage of mitochondria with respect to the concentration of oleate. The latent ATPase was abolished also by the addition of a sufficient amount of BSA. 5. The action of oleate on the phosphorylation sequence of mitochondria was discussed on the basis of the present findings.</p>

en-copyright=
kn-copyright=

en-aut-name=YamamotoGoki
en-aut-sei=Yamamoto
en-aut-mei=Goki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University
END

start-ver=1.4
cd-journal=joma
no-vol=18
cd-vols=
no-issue=4
article-no=
start-page=239
end-page=240
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1964
dt-pub=196408
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of cystathionase on isovalthine
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>In the course of studies on the cleavage reaction of S-(isopropylcarboxymethyl) glutathione (GSIV) into isovalthine in kidney homogenate or glutathionase preparation, it has sometimes been observed that the amount of isovalthine formed is far less than that of GSIV decomposed&#185;. Furthermore, when such reaction mixture is analyzed on an automatic amino acid analyzer, prominent peak corresponding to the reasonable amount of S-(isopropy1carboxymethyl)cysteinylglycine which is an expected intermediate of the GSIV cleavage reaction cannot be found up to 400 effluent ml. Though several reasons may be considered for the explanation of the above curious phenomenon, the effect of cystathionase on isovalthine is at first examined here. But the result was negative. L- and L-Alloisovalthineused as substrate were prepared by the method of OHMORI&#178;. Homoserine and purified cystathionase in ammonium sulfate solution prepared according to the method of GREENBERGB&#179; were kindly furnished by Prof. M. Suda of Osaka University. Incubation mixture contains 0.1 ml of enzyme solution, 1.0 ml of 0.2 M
borate buffer (pH 8.0) containing 2×10-&#179;M cysteine, 0.lml of 0.1 M substrate, and 0.8ml of deionized water containing 5×10-4M EDTA. The mixture was shaken at 37°C for 30 minutes in the air. The reaction was terminated by
adding 2ml of 10% trichloroacetic acid and the &#945;-keto acids formed were determined by the method of FRIEDEMANN and HAUGEN4 with a following modification: toluene extract was washed once with 8 ml of 10% sodium sulfate. The results obtained are summarized in Table l. When the reaction mixtures are analyzed before or after incubation on an automatic amino acid analyzer, the amount of L- or L-Alloisovalthine is found to be unchanged. Furthermore, as indicated in Table 1, L-isovalthine showed no inhibitory effect on the homoserine cleavage by cystathionase. Since amino acid oxidases have already been reported to have no effect on isovalthine&#179;, the curious phenomenon above cited may have to be explained by other reaction mechanism such as transpeptipation reaction.</p>

en-copyright=
kn-copyright=

en-aut-name=UbukaToshihiko
en-aut-sei=Ubuka
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HoriuchiKatsumi
en-aut-sei=Horiuchi
en-aut-mei=Katsumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ShimomuraTakehira
en-aut-sei=Shimomura
en-aut-mei=Takehira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AzumiTsukasa
en-aut-sei=Azumi
en-aut-mei=Tsukasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=6
article-no=
start-page=317
end-page=331
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1962
dt-pub=196212
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Mitochondrial Swelling and Uncoupling Activity of Long-Chain Fatty Acids
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The effect of various fatty acids on the swelling-contraction and oxidative phosphorylation of mitochondria from rat liver and Ehrlich ascites tumor cell have been studied and the results are as follows: 1. The swelling of rat liver mitochondria is induced by fatty acid. The extent of this uncoupling action is in the descending order of myristate, laurate, parlmitate, stearate and behenate in saturated fatty acid and linoleate, linoleneate, richinoleate and oleate in the unsaturated fatty acid. This swelling action is stronger with unsaturated fatty acids than that of saturated ones and cis form is stronger than trans form. 2. The uncoupling oxidative phosphorylation of rat liver mitochondria is also observed with these fatty acids and the activities are proportional to the degree of the swelling action. 3. The degree of swelling of rat liver mitochondria is proportional to the concentration of oleate and is inhibited by anaerobiosis and respiratory inhibitor except amytal. 4. The mitochondria swollen by fatty acid can be recontracted reversibly by ATP, Mg++ and bovine serum albumin. 5. The swelling action of sodium oleate is the strongest on mitochondria from rat liver, followed by those from the liver of Ehrlich ascites tumor bearing mouse, Ehrlich ascites tumor cells and solid Ehrlich tumor cells. 6. Sodium oleate inhibits the incorporation of 32p into ATP, ADP, GTP and UDPG in mitochondria.</p>

en-copyright=
kn-copyright=

en-aut-name=UtsumiKozo
en-aut-sei=Utsumi
en-aut-mei=Kozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OharaSachiko
en-aut-sei=Ohara
en-aut-mei=Sachiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamamotoGoki
en-aut-sei=Yamamoto
en-aut-mei=Goki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=InabaKozo
en-aut-sei=Inaba
en-aut-mei=Kozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=UrakamiHiroyuki
en-aut-sei=Urakami
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YamamotoMichio
en-aut-sei=Yamamoto
en-aut-mei=Michio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=54
cd-vols=
no-issue=2
article-no=
start-page=49
end-page=56
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2000
dt-pub=200004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Possible postsynaptic action of aminoglycosides in the frog rectus abdominis.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The present study was undertaken to investigate the postsynaptic effects of aminoglycosides on contractions evoked by acetylcholine (ACh), KCl, electrical field stimulation (EFS) and Na(+)- and Ca(2+)-free Ringer solution with 0.2 mM Na2 EDTA (NaFCaFR) in the isolated frog rectus abdominis. Neomycin inhibited contraction elicited by ACh, NaFCaFR, and EFS at the higher frequencies (8 and 10 Hz) but not those elicited by KCl and EFS at the lower frequencies (2, 3 and 5 Hz). D-tubocurarine inhibited ACh-induced contractions in a concentration-dependent manner. In addition, drug reduced EFS-evoked contractions to a limited extent. Lower concentrations (10(-5), 5 x 10(-5), 10(-4), 2 x 10(-4) and 3 x 10(-4) M) but not higher concentrations (4 x 10(-4) and 5 x 10(-4) M) of methoxyverapamil exhibited a concentration-dependent inhibitory action on NaFCaFR-induced contractions. Similar inhibitions of the same type of contraction were displayed by aminoglycosides (neomycin, streptomycin, netilmycin, gentamycin and amikacin). These results suggest that in addition to their antagonistic action on nicotinic receptors in the frog rectus abdominis, aminoglycosides may exert stabilizing effects on some functional components contributing to contractions at the membrane.</p>

en-copyright=
kn-copyright=

en-aut-name=KaratasYusuf
en-aut-sei=Karatas
en-aut-mei=Yusuf
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ErgunYusuf
en-aut-sei=Ergun
en-aut-mei=Yusuf
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=GocmenCemil
en-aut-sei=Gocmen
en-aut-mei=Cemil
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SecilmisAta
en-aut-sei=Secilmis
en-aut-mei=Ata
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SingirikErgin
en-aut-sei=Singirik
en-aut-mei=Ergin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=DikmenAtilla
en-aut-sei=Dikmen
en-aut-mei=Atilla
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=BayasalFiruz
en-aut-sei=Bayasal
en-aut-mei=Firuz
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Cukurova University

affil-num=2
en-affil=
kn-affil=Cukurowa University

affil-num=3
en-affil=
kn-affil=Cukurova University

affil-num=4
en-affil=
kn-affil=Cukurova University

affil-num=5
en-affil=
kn-affil=Cukurova University

affil-num=6
en-affil=
kn-affil=Cukurova University

affil-num=7
en-affil=
kn-affil=Cukurowa University
en-keyword=aminoglycoside
kn-keyword=aminoglycoside
en-keyword=voltage sensor
kn-keyword=voltage sensor
en-keyword=sodium and calcium free Ringer solution
kn-keyword=sodium and calcium free Ringer solution
en-keyword=frog rectus abdominis
kn-keyword=frog rectus abdominis
en-keyword=contoraction
kn-keyword=contoraction
END

start-ver=1.4
cd-journal=joma
no-vol=54
cd-vols=
no-issue=2
article-no=
start-page=57
end-page=65
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2000
dt-pub=200004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Purification, identification and phosphorylation of annexin I from rat liver mitochondria.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Annexin was purified from rat liver mitochondria to an apparent homogeneity with a molecular weight of 35 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified mitochondrial annexin (AXmito) was identified as annexin I by an immunoblot analysis using anti-annexin I antibody. The inhibitory effect of AXmito I on porcine pancreatic phospholipase A2 activity was as potent as that of bovine lung annexin I. The presence of annexin I in mitochondria was confirmed by an electron-microscopic study. AXmito I was shown to be phosphorylated by intrinsic protein tyrosine kinases on its tyrosine residues. This annexin was also phosphorylated by protein kinase C.</p>

en-copyright=
kn-copyright=

en-aut-name=YoshiiKenji
en-aut-sei=Yoshii
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SugimotoKatsuyoshi
en-aut-sei=Sugimoto
en-aut-mei=Katsuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TaiYuji
en-aut-sei=Tai
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KonishiRyoji
en-aut-sei=Konishi
en-aut-mei=Ryoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TokudaMasaaki
en-aut-sei=Tokuda
en-aut-mei=Masaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Kagawa Medical University

affil-num=2
en-affil=
kn-affil=Kagawa Medical University

affil-num=3
en-affil=
kn-affil=Kagawa Medical University

affil-num=4
en-affil=
kn-affil=Kagawa Medical University

affil-num=5
en-affil=
kn-affil=Kagawa Medical University
en-keyword=annexin
kn-keyword=annexin
en-keyword=mitochondria
kn-keyword=mitochondria
en-keyword=protein tyrosine kinases
kn-keyword=protein tyrosine kinases
en-keyword=protein kinase C
kn-keyword=protein kinase C
en-keyword=Phospholipase A2
kn-keyword=Phospholipase A2
END

start-ver=1.4
cd-journal=joma
no-vol=45
cd-vols=
no-issue=5
article-no=
start-page=295
end-page=299
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1991
dt-pub=199110
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of beta-adrenergic blocking agents on specific binding of [3H]D-Ala2-Met5-enkephalinamide and [3H]naloxone.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>To gain further insight into the central nervous system (CNS)-action of beta-adrenergic blocking agents (beta-blockers), we examined the effects of various kinds of beta-blockers on opioid receptors (Op-Rs) using radiolabeled receptor assay (RRA). We demonstrated that beta-blockers are competitively bound to Op-Rs in the CNS. Sodium index of beta-blockers in [3H]naloxone binding study indicated that beta-blockers had the mixed agonist-antagonist activity of opiates. The relative potency of beta-blockers in opioid RRA was negatively correlated with their membrane stabilizing activity. Neither beta-blocking activity nor intrinsic sympathomimetic activity was correlated with IC50 values of beta-blockers in opioid RRA. While it is widely accepted that beta-blockers have a tranquilizing activity, a part of the tranquilizing action of beta-blockers may be mediated through Op-Rs in the CNS. Although beta-blockers may have effects on their own receptors (beta-receptors) in the CNS, the more precise mechanisms of central action of these drugs must be further investigated.</p>

en-copyright=
kn-copyright=

en-aut-name=TakayamaHaruhiko
en-aut-sei=Takayama
en-aut-mei=Haruhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OgawaNorio
en-aut-sei=Ogawa
en-aut-mei=Norio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AsanumaMasato
en-aut-sei=Asanuma
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HirataHiroshi
en-aut-sei=Hirata
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OguraToshio
en-aut-sei=Ogura
en-aut-mei=Toshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OtaZensuke
en-aut-sei=Ota
en-aut-mei=Zensuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama  University

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama  University

affil-num=6
en-affil=
kn-affil=Okayama  University
en-keyword=?-blocker
kn-keyword=?-blocker
en-keyword=opioid receptor
kn-keyword=opioid receptor
en-keyword=membrane stabilizing activity
kn-keyword=membrane stabilizing activity
en-keyword=sodium index
kn-keyword=sodium index
END

start-ver=1.4
cd-journal=joma
no-vol=58
cd-vols=
no-issue=1
article-no=
start-page=45
end-page=49
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2004
dt-pub=200402
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effectiveness of a nonsteroidal anti-inflammatory drug for nocturia on patients with benign prostatic hyperplasia: a prospective non-randomized study of loxoprofen sodium 60 mg once daily before sleeping.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We explored the effectiveness of loxoprofen sodium (loxoprofen), which is the most common non-steroidal anti-inflammatory drug (NSAID) in Japan, for patients with benign prostatic hyperplasia (BPH) complaining of nocturia. A total of 93 BPH patients aged 49-84 years were enrolled in the study. These patients had received standard drug therapy with alpha1-blocker for BPH, followed by anticholinergic drugs, hypnotics, tricyclic antidepressants, and/or antiduretic hormone, but they still complained about 2 or more episodes of nocturia. They each took a single 60-mg tablet of loxoprofen prior to sleeping at night for 14 days in addition to their BPH treatments. The effects were assessed by questionnaire before and after treatment as excellent (nocturia disappeared or decreased by 2 or more voids/night), improved (nocturia decreased by 1 void/night), unchanged, or worsened (nocturia increased). Nocturia improved or disappeared in 74.2% of patients: excellent, improved, unchanged, and worsened results were obtained in 37.6%, 36.6%, 21.5%, and 4.3% of patients, respectively. The effects were better in patients whose baseline nocturia was &#62; 2 times than in those with a lesser frequency at enrollment (P = 0.04). Loxoprofen can be an effective and useful treatment option for patients with BPH complaining of refractory nocturia.</p>

en-copyright=
kn-copyright=

en-aut-name=ArakiTohru
en-aut-sei=Araki
en-aut-mei=Tohru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YokoyamaTeruhiko
en-aut-sei=Yokoyama
en-aut-mei=Teruhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KumonHiromi
en-aut-sei=Kumon
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Araki Urological Clinic

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University
en-keyword=nocturia
kn-keyword=nocturia
en-keyword=loxoprofen sodium
kn-keyword=loxoprofen sodium
en-keyword= non-steroidal anti-inflammatory drugs (NSAIDs)
kn-keyword= non-steroidal anti-inflammatory drugs (NSAIDs)
END

start-ver=1.4
cd-journal=joma
no-vol=33
cd-vols=
no-issue=3
article-no=
start-page=205
end-page=211
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=197906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Properties of erythrocyte catalase from heterozygotes for Japanese type acatalasemia.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The level of blood catalase activity in heterozygotes for Japanese type acatalasemia was demonstrated to be about half of normal levels by means of titration and spectrophotometric methods. A distribution plot of catalase activities in heterozygous blood was completely separate from that of normal blood. Comparative analysis of the partially purified erythrocyte catalase preparations obtained from normal and heterozygous individuals revealed no distinct differences between them regarding stability to heat, sodium dodecyl sulfate and some enzyme inhibitors or pH dependency. The erythrocyte catalase in heterozygotes for Japanese type acatalasemia contains about half the normal specific activity and as stable as that in normal individuals.</p>

en-copyright=
kn-copyright=

en-aut-name=OgataMasana
en-aut-sei=Ogata
en-aut-mei=Masana
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MizugakiJunko
en-aut-sei=Mizugaki
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University
en-keyword=erythrocye catalase
kn-keyword=erythrocye catalase
en-keyword=heterozygote for Jaoanese type acatalasemia
kn-keyword=heterozygote for Jaoanese type acatalasemia
en-keyword=stability
kn-keyword=stability
END

start-ver=1.4
cd-journal=joma
no-vol=55
cd-vols=
no-issue=2
article-no=
start-page=129
end-page=132
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2001
dt-pub=200104
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Severe fenitrothion poisoning complicated by rhabdomyolysis in psychiatric patient.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Non-traumatic rhabdomyolysis associated with organophosphate intoxication has not been generally reported. We report here in a severe case of fenitrothion poisoning complicated by rhabdomyolysis. A 43-year-old woman ingested approximately 100 ml of fenitrothion emulsion (50%) in an attempt to commit suicide. On day 3 after admission, her creatine phosphokinase (CPK) peaked at 47,762 IU/L. She received supportive treatment included sodium bicarbonate and fluid resuscitation. However, muscarinic symptoms including excessive miosis and salivation developed on day 5 when her CPK levels decreased. The delay in cholinergic symptoms might have been due to the trihexyphenidyl she took with the antipsychotic drugs. Fortunately, the present patient recovered from the acute cholinergic crisis, and acute renal failure was prevented by early diagnosis. This is a case of organophosphate poisoning complicated by rhabdomyolysis in a psychiatric patient. The masking of acute cholinergic symptoms should be taken into consideration in such patients.</p>

en-copyright=
kn-copyright=

en-aut-name=FutagamiKoujiro
en-aut-sei=Futagami
en-aut-mei=Koujiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HiranoNaofumi
en-aut-sei=Hirano
en-aut-mei=Naofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=IimoriEmiko
en-aut-sei=Iimori
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MotomuraKenichi
en-aut-sei=Motomura
en-aut-mei=Kenichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=IdeMichiko
en-aut-sei=Ide
en-aut-mei=Michiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KataokaYasuhumi
en-aut-sei=Kataoka
en-aut-mei=Yasuhumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=ArakiHiroaki
en-aut-sei=Araki
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=GomitaYutaka
en-aut-sei=Gomita
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=OishiRyozo
en-aut-sei=Oishi
en-aut-mei=Ryozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Kyushu University

affil-num=3
en-affil=
kn-affil=Kyushu University

affil-num=4
en-affil=
kn-affil=Kyushu University

affil-num=5
en-affil=
kn-affil=Kyushu University

affil-num=6
en-affil=
kn-affil=Kyushu University

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Kyushu University
en-keyword=fenitrothion
kn-keyword=fenitrothion
en-keyword=organophosphate poisoning
kn-keyword=organophosphate poisoning
en-keyword=rhabdomyolysis
kn-keyword=rhabdomyolysis
en-keyword=psychiatric patient
kn-keyword=psychiatric patient
END

start-ver=1.4
cd-journal=joma
no-vol=55
cd-vols=
no-issue=4
article-no=
start-page=245
end-page=252
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2001
dt-pub=200108
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Significance of adrenomedullin under cardiopulmonary bypass in children during surgery for congenital heart disease.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>To elucidate the effect of adrenomedullin (AM) on fluid homeostasis under cardiopulmonary bypass (CPB), we investigated the serial changes in plasma AM and other parameters related to fluid homeostasis in 13 children (average age, 28.2 months) with congenital heart disease during cardiac surgery under CPB. Arterial blood and urine samples were collected just after initiation of anesthesia, just before commencement of CPB, 10 min before the end of CPB, 60 min after CPB, and 24 h after operation. Plasma AM levels increased significantly 10 min before the end of CPB and decreased 24 h after operation. Urine volume increased transiently during CPB, which paralleled changes in AM. Simple regression analysis showed that plasma AM level correlated significantly with urinary vasopressin, urine volume, urinary sodium excretion, and plasma osmolarity. Stepwise regression analysis indicated that urine volume was the most significant determinant of plasma AM levels. Percent rise in AM during CPB relative to control period correlated with that of plasma brain natriuretic peptide (r = 0.57, P &#60; 0.01). Our results suggest that AM plays an important role in fluid homeostasis under CPB in cooperation with other hormones involved in fluid homeostasis.</p>

en-copyright=
kn-copyright=

en-aut-name=TakeuchiMamoru
en-aut-sei=Takeuchi
en-aut-mei=Mamoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MoritaKiyoshi
en-aut-sei=Morita
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=IwasakiTatsuo
en-aut-sei=Iwasaki
en-aut-mei=Tatsuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TodaYuichiro
en-aut-sei=Toda
en-aut-mei=Yuichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OeKatsunori
en-aut-sei=Oe
en-aut-mei=Katsunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TagaNaoyuki
en-aut-sei=Taga
en-aut-mei=Naoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HirakawaMasahisa
en-aut-sei=Hirakawa
en-aut-mei=Masahisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University
en-keyword=adrenomedullin
kn-keyword=adrenomedullin
en-keyword=cardiopulmonary bypass
kn-keyword=cardiopulmonary bypass
en-keyword=vasopressin
kn-keyword=vasopressin
en-keyword=pediatric cardiac surgery
kn-keyword=pediatric cardiac surgery
en-keyword=brain natriuretic peptide
kn-keyword=brain natriuretic peptide
END

start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=4
article-no=
start-page=171
end-page=176
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=200208
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Improvements in the measurement of stool decay-accelerating factor in the detection of colorectal cancer.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We have previously developed an enzyme-linked immunosorbent assay (ELISA) to measure stool decay-accelerating factor (DAF) and found that stool DAF concentrations were significantly elevated in patients with colorectal cancer, suggesting that the measurement of stool DAF may be valuable for the detection of colorectal cancer. In order to refine the assay for the measurement of stool DAF, we investigated 1) effects of centrifugation of stool samples, 2) effects of detergents, and 3) adequate combination of various anti-DAF monoclonal antibodies for the ELISA system using only monoclonal antibodies. We found that high-speed centrifugation could be omitted and that only the removal of large undigested food residues by centrifugation of short duration in a low-speed benchtop microcentrifuge sufficed to adequately prepare the stool samples. Addition of 2 detergents, octyl beta-glucoside and sodium deoxycholate, known to solubilize glycosyl-phosphatidylinositol-anchored proteins such as DAF, did not influence stool DAF values. By using 2 mouse anti-DAF monoclonal antibodies (clone 4F11 and 1C6), we were able to achieve a stable ELISA for the measurement of stool DAF using a uniform source of antibodies. The results should allow us to consistently apply the DAF assay for routine use in the detection of colorectal cancer.</p>

en-copyright=
kn-copyright=

en-aut-name=OhyaShogen
en-aut-sei=Ohya
en-aut-mei=Shogen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MizunoMotowo
en-aut-sei=Mizuno
en-aut-mei=Motowo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KawadaMikihiro
en-aut-sei=Kawada
en-aut-mei=Mikihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NasuJunichirou
en-aut-sei=Nasu
en-aut-mei=Junichirou
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ShimomuraHiroyuki
en-aut-sei=Shimomura
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YamamotoKazuhide
en-aut-sei=Yamamoto
en-aut-mei=Kazuhide
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=FujitaTeizou
en-aut-sei=Fujita
en-aut-mei=Teizou
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University 

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University 

affil-num=4
en-affil=
kn-affil=Okayama University 

affil-num=5
en-affil=
kn-affil=Okayama University 

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Fukushima Medical College

affil-num=9
en-affil=
kn-affil=Okayama University
en-keyword=decay-accelerating factor (DAF)
kn-keyword=decay-accelerating factor (DAF)
en-keyword=colorectal cancer
kn-keyword=colorectal cancer
en-keyword=enzyme-linked immunosorbent assay (ELISA). monoclonal sntibodies
kn-keyword=enzyme-linked immunosorbent assay (ELISA). monoclonal sntibodies
END

start-ver=1.4
cd-journal=joma
no-vol=53
cd-vols=
no-issue=1
article-no=
start-page=55
end-page=59
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1999
dt-pub=199902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Hydrocortisone Sodium Succinate Suppressed Production of Interleukin-10 by Human Peripheral Blood Mononuclear Cells: Clinical Significance
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Corticoids are well known for their immunosuppressive properties. Interleukin-10 (IL-10) is an intrinsic antiinflammatory peptide in immune diseases, originally identified as cytokine synthesis inhibitory factor. We examined the effect of hydrocortisone sodium succinate (HSS) on the production of IL-10 by human peripheral blood mononuclear cells (PBMCs). PBMCs from healthy volunteers and cancer-burden patients were preincubated separately with or without HSS for 1 h, then stimulated with 5 microg/ml lipopolysaccharide (LPS). Production of IL-10 by human PBMCs was detected with LPS stimulation and its production was higher in cancer-burden patients than in normal volunteers, although this was not statistically significant. HSS suppressed production of IL-10 by LPS-stimulated PBMCs in a dose-dependent manner both in normal volunteers and in cancer-burden patients. These results indicate that, in addition to their antiinflammatory properties, corticoids act to restore the immunosuppressive states even in cancer-burden states</p>

en-copyright=
kn-copyright=

en-aut-name=KohkaHideo
en-aut-sei=Kohka
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IwagakiHiromi
en-aut-sei=Iwagaki
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YoshinoTadashi
en-aut-sei=Yoshino
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KobashiKenta
en-aut-sei=Kobashi
en-aut-mei=Kenta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SaitoShinnya
en-aut-sei=Saito
en-aut-mei=Shinnya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=IsozakiHiroshi
en-aut-sei=Isozaki
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakakuraNorihisa
en-aut-sei=Takakura
en-aut-mei=Norihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TanakaNoriaki
en-aut-sei=Tanaka
en-aut-mei=Noriaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University
en-keyword=steroid
kn-keyword=steroid
en-keyword=interleukin-10
kn-keyword=interleukin-10
en-keyword=cancer-burden state
kn-keyword=cancer-burden state
END

start-ver=1.4
cd-journal=joma
no-vol=53
cd-vols=
no-issue=5
article-no=
start-page=209
end-page=215
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1999
dt-pub=199910
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Evidence that the nitrergic neurotransmitter and endothelium-derived relaxing factor might be S-nitrosothiols in the mouse corpus cavernosum.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The effects of thimerosal, a sulfhydryl oxidizing agent on nitrergic, endothelium-dependent and -independent relaxations were investigated to examine the possibility that the nitrergic neurotransmitter and endothelium-derived relaxing factor (EDRF) could be S-nitrosothiol or free nitric oxide (NO) in the isolated mouse corpus cavernosum. Thimerosal (5 x 10(-6)-2 x 10(-5) M) inhibited or almost abolished electrical field stimulation--(EFS, 30V, 0.5 ms, 15 sec, 1, 2, 4, 8, 16 Hz), acetylcholine--(ACh, 5 x 10(-8)-1.25 x 10(-6) M), glyceryl trinitrate--(GTN, 3 x 10(-7)-3 x 10(-6) M), and S-nitrosoglutathione--(GSNO, 5 x 10(-6)-1.25 x 10(-4) M) induced relaxations. Thiomerosal inhibition seems to be specific to L-arginine NO pathways since it had no effect on acidified sodium nitrite--(10(-4)-5 x 10(-4) M), photoactivated sodium nitrite--(2 x 10(-4) M), isoprenaline--(10(-6) M), or papaverine--(10(-4) M) elicited relaxations. Moreover, the inhibitory effect of thimerosal on the nitrergic, ACh- or GTN-induced relaxations were partly reversed by sulfhydryl-containing compounds, L-cysteine (10(-3) M), dithiothreitol (10(-3) M), or glutathione (10(-3) M). However L-methionine (10(-3) M), which contains a methyl group on the sulphur atom, failed to restore the thimerosal inhibition. Thimerosal did not change the contraction produced by 10(-4) M NG-nitro-L-arginine methyl ester. These findings indicate that the nitrergic neurotransmitter as well as EDRF may not be free NO but NO-transferring molecules, probably S-nitrosothiols, in the mouse corpus cavernosum.</p>

en-copyright=
kn-copyright=

en-aut-name=BuyukafsarKansu
en-aut-sei=Buyukafsar
en-aut-mei=Kansu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=GocmenCemil
en-aut-sei=Gocmen
en-aut-mei=Cemil
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SecilmisAta
en-aut-sei=Secilmis
en-aut-mei=Ata
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KaratasYusuf
en-aut-sei=Karatas
en-aut-mei=Yusuf
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=GokturkSinem
en-aut-sei=Gokturk
en-aut-mei=Sinem
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KalyoncuNuri Ihsan
en-aut-sei=Kalyoncu
en-aut-mei=Nuri Ihsan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Mersin University

affil-num=2
en-affil=
kn-affil=Cukurova University

affil-num=3
en-affil=
kn-affil=Cukurova University

affil-num=4
en-affil=
kn-affil=Cukurova University

affil-num=5
en-affil=
kn-affil=Cukurova University

affil-num=6
en-affil=
kn-affil=Karadeniz Technical University
en-keyword=nitric oxide
kn-keyword=nitric oxide
en-keyword=endothelium-derived relaxing factor
kn-keyword=endothelium-derived relaxing factor
en-keyword=nitrergic neurotransmitter
kn-keyword=nitrergic neurotransmitter
en-keyword=thimerosal
kn-keyword=thimerosal
en-keyword=corpus cavernosum
kn-keyword=corpus cavernosum
en-keyword=S-nitrosothiols
kn-keyword=S-nitrosothiols
END

start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=3
article-no=
start-page=179
end-page=190
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1985
dt-pub=198506
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Evaluation of Talbot's Safety Zone of Infusion Volume and Osmolality in Infusion Therapy for Decompensated Liver Cirrhosis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>Problems with infusion therapy for correcting fluid and sodium imbalance in decompensated liver cirrhosis (DLC) were investigated by establishing the safety zone of Talbot et al. for parenteral fluid therapy in 4 DLC patients infused with over 900 ml of fluid each day for at least 9 days. The safety zone was different in each case. The safe infusion volume decreased and the safe electrolyte concentration shifted to a lower osmolality when there was ascites with renal failure than ascites without renal failure. Infusion therapy was performed without deterioration of the water and sodium balance in those patients whose infusion volume and fluid osmolality were in the safety zone. In contrast, ascites retention increased and peripheral edema appeared in patients whose infusion volume and osmolality were out of the safety zone. Therefore, the safety zone should be determined repeatedly during infusion therapy.</p>

en-copyright=
kn-copyright=

en-aut-name=YuasaShiro
en-aut-sei=Yuasa
en-aut-mei=Shiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ItoshimaTatsuya
en-aut-sei=Itoshima
en-aut-mei=Tatsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OnoRyosaku
en-aut-sei=Ono
en-aut-mei=Ryosaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NagashimaHideo
en-aut-sei=Nagashima
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University
en-keyword=decompensated liver cirrhosis
kn-keyword=decompensated liver cirrhosis
en-keyword=infusion therapy
kn-keyword=infusion therapy
en-keyword=ascites
kn-keyword=ascites
en-keyword=hepatorenal syndrome
kn-keyword=hepatorenal syndrome
END

start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=3
article-no=
start-page=217
end-page=220
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1985
dt-pub=198506
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of pentazocine and concomitant clonidine on opioid receptors in the rat brain.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The changes in opioid receptors (Op-R) caused by repeated administration of pentazocine and the effect of concomitant clonidine were investigated. Binding of [3H] naloxone was markedly decreased in the absence of Na+, but was increased in the presence of Na+ in the diencephalon-mesencephalon of chronic pentazocine-treated rats. No significant changes were observed in the cerebral cortex of pentazocine-treated rats. The pentazocine-induced changes in Op-R were abolished by the concurrent use of clonidine, an alpha-adrenergic agonist, which has been shown to relieve the withdrawal symptoms of morphine. This result indicated that the behavioral action of clonidine can also be observed at the Op-R level.</p>

en-copyright=
kn-copyright=

en-aut-name=OgawaNorio
en-aut-sei=Ogawa
en-aut-mei=Norio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HiroseYukiko
en-aut-sei=Hirose
en-aut-mei=Yukiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurodaHiroo
en-aut-sei=Kuroda
en-aut-mei=Hiroo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakayamaHaruhiko
en-aut-sei=Takayama
en-aut-mei=Haruhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University 

affil-num=4
en-affil=
kn-affil=Okayama University
en-keyword=opioid receptors
kn-keyword=opioid receptors
en-keyword=pentazocine
kn-keyword=pentazocine
en-keyword=clonidine
kn-keyword=clonidine
en-keyword=naloxone binding
kn-keyword=naloxone binding
en-keyword=sodium effect
kn-keyword=sodium effect
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=3
article-no=
start-page=193
end-page=204
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=195810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=An analytical study on the reduction of neotetrazolium chloride by the terminal electron transport system
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>In order to determine the steps with which the reaction of neotetrazolium chloride reduction conjugates in the terminal electron transport system, an analytical study on the neotetrazolium reduction by tissue homogenates was carried out using various substrates such as sodium succinate, p-phenylenediamine, sodium malate, sodium &#945;-glutamate and DPN, and inhibitors such as sodium malonate, potassium cyanide and antimycin A, as the results the following conclusions were drawn. 1. The reaction of neotetrazolium reduction by tissue homogenate
using sodium succinate as substrate is mainly the succinoxidase system reaction; and the reaction takes place conjugating about 50 per cent in the step of the succinic dehydrogenase system (succinic dehydrogease, cytochrome b and cytochrome C1), of these about 15 per cent conjugates in the step prior to the antimycin A sensitive step and 35 per cent in the step itself; and about 50 per cent in the step of cytochrome c oxidase. 2. In the case using p-phenylenediamine as substrate the reaction of neotetrazolium reduction is the reaction due to the activity of cytochrome c-cytochrome oxidase system; and when p-phenylenediamine is used with the sufficient amount of cytochrome c, the reaction appears to be dependent on cytochrome c oxidase activity. Neotetrazolium reduction in all these reactions takes place conjugating in the step of cytochrome c oxidase. 3. In the case where DPN and substrates taking DPN as a coenzyme are used, the reaction of neotetrazolium reduction is mainly the reaction
conjugating at the step below antimycin A sensitive step in the DPNHcytochrome c reductase system (flavoprotein, cytochrome b and cytochrome c;), probably with the flavoprotein of DPNH-dehydrogenase. 4. Endogenous dehydrogenase reactions are the sum total reactions conjugating at the steps prior to the antimycin A sensitive step in the terminal electron transport system and with other various reduction systems which are not inhibited by antimycin A.</p>

en-copyright=
kn-copyright=

en-aut-name=OdaTakuzo
en-aut-sei=Oda
en-aut-mei=Takuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkazakiHiroaki
en-aut-sei=Okazaki
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=2
article-no=
start-page=93
end-page=111
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=195807
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of histamine releasers and of anti-inflammatory drugs on the egg-white edema of rat's hind paws in relation to skin histamine
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>1. A method was described for a fairly accurate judgement of the effect of drugs inhibiting the edema in hind paws of a rat caused by local injection of egg white.
2. The degree of inhibition of egg-white edema by single doses of sinomenine, compound 48/80, or dextran was in parallel with histamine reduction in skin and other tissues of the paws (and the skin of abdomen), although prevention of the edema by prolonged treatment with sinomenine was incomplete even when the releasable histamine of the skin was practically exhausted. 3. Sodium salicylate, aminopyrine, butazolidine sodium, cortisone, and guaiazulene were capable of inhibiting egg-white edema without modifying the content of skin histamine. These drugs and a small dose of phenergan potentiated the inhibition by dextran of egg-white edema and inhibited the release of histamine by dextran. These actions lasted for
over 24 hours with the exception of guaiazulene. 4. Irgapyrin and a large dose of phenergan, which possess actions of histamine release and of histamine release inhibition and also antihistaminic action, caused a slight reduction of skin histamine and a comparatively marked inhibition of the edema. 5. In adrenalectomized or hypophysectomized rats, the edema-inhibiting effect of salicylate and aminopyrine decreased but that of cortisone
increased. The effect of guaiazulene remained unchanged. 6. The observations that inhibition of egg-white edema is caused by (a) histamine releasers, (b) histamine-release inhibitor, and (c) drugs exerting both histamine release and inhibition of the release were discussed with the consideration to a relationship between egg-white edema and
skin histamine.</p>

en-copyright=
kn-copyright=

en-aut-name=IrinoShozo
en-aut-sei=Irino
en-aut-mei=Shozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=52
cd-vols=
no-issue=5
article-no=
start-page=279
end-page=283
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1998
dt-pub=199810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Expression of amiloride-sensitive sodium channel in rat eye.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>To study the expression of the amiloride-sensitive sodium channel, a putative mechano-receptor in the rat eye, reverse transcriptase-polymerase chain reaction and in situ hybridization were done. The gene for the alpha subunit of the amiloride-sensitive sodium channel was shown by polymerase chain reaction to be expressed in mRNA isolated from the whole eye tissue. In situ hybridization demonstrated that the gene was expressed in basal layers of the corneal and conjunctival epithelium, ciliary epithelial cells, lens epithelial cells at the equator, retinal and iris pigment epithelial cells, ganglion cells and cells in the inner and outer nuclear layers of the retina. The results suggest that the amiloride-sensitive sodium channel plays a role in maintaining sodium balance as well as in possible mechanosensation in these ocular tissues.</p>

en-copyright=
kn-copyright=

en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University
en-keyword=amiloride-sensitive sodium channel
kn-keyword=amiloride-sensitive sodium channel
en-keyword=eye
kn-keyword=eye
en-keyword=insitu hybridzation
kn-keyword=insitu hybridzation
en-keyword=mechanosensation
kn-keyword=mechanosensation
en-keyword=polymerase chain reaction
kn-keyword=polymerase chain reaction
END

start-ver=1.4
cd-journal=joma
no-vol=52
cd-vols=
no-issue=6
article-no=
start-page=325
end-page=329
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1998
dt-pub=199812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Can POSSUM, a Scoring System for Perioperative Surgical Risk, Predict Postoperative Clinical Course ?
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>POSSUM, a Physiological and Operative Severity Score for the enUmeration of Mortality and morbidity, is a scoring system which assesses perioperative surgical risks (Copeland GP et al.: Br J Surg, 1991, Vol 78, 356-360). The POSSUM scoring system consists of two categories of assessment to assess the risk of surgery. A 12-factor (age, cardiac status, pulse rate, systolic blood pressure, respiratory status, Glasgow Coma Score, serum concentration of urea, potassium and sodium, hemoglobin concentration, white cell count and findings on electrocardiography) and 4-grade physiological score (PS) were developed. This was combined with a 6-factor (type of surgical procedure, number of procedures, blood loss, peritoneal soiling, presence of malignancy and mode of surgery) and 4-grade operative severity score (OSS). The present paper attempts to validate it retrospectively. Postoperative hospitalization period and duration of antibiotics administration were both significantly correlated with OSS, but not with PS. These results suggest that the POSSUM scoring system is useful for predicting the postoperative clinical course.</p>

en-copyright=
kn-copyright=

en-aut-name=GotohdaNaoto
en-aut-sei=Gotohda
en-aut-mei=Naoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IwagakiHiromi
en-aut-sei=Iwagaki
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ItanoSatoshi
en-aut-sei=Itano
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HorikiSadayuki
en-aut-sei=Horiki
en-aut-mei=Sadayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=FujiwaraToshiya
en-aut-sei=Fujiwara
en-aut-mei=Toshiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SaitoShinya
en-aut-sei=Saito
en-aut-mei=Shinya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HizutaAkio
en-aut-sei=Hizuta
en-aut-mei=Akio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=IsozakiHiroshi
en-aut-sei=Isozaki
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TakakuraNorihisa
en-aut-sei=Takakura
en-aut-mei=Norihisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=TeradaNorihiko
en-aut-sei=Terada
en-aut-mei=Norihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=TanakaNoriaki
en-aut-sei=Tanaka
en-aut-mei=Noriaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University 

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Terada General Hospital

affil-num=4
en-affil=
kn-affil=Terada General Hospital

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University 

affil-num=7
en-affil=
kn-affil=Okayama University 

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama University

affil-num=10
en-affil=
kn-affil=Terada General Hospital

affil-num=11
en-affil=
kn-affil=Okayama University
en-keyword=surgical risk
kn-keyword=surgical risk
en-keyword=Physiological and Operative Severity Source for the enUmeration of Mortality and morbidity
kn-keyword=Physiological and Operative Severity Source for the enUmeration of Mortality and morbidity
END

start-ver=1.4
cd-journal=joma
no-vol=35
cd-vols=
no-issue=1
article-no=
start-page=27
end-page=35
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1981
dt-pub=198102
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A perifusion method for examining arginine vasopressin (AVP) release from hypothalamo-neurohypophyseal system.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A perifusion method has been developed using rat hypothalamo-neurohypophyseal system (HNS) or neural lobe to investigate the control mechanism of arginine vasopressin (AVP) release. A specific radioimmunoassay (RIA) for AVP was developed to measure AVP in perifusion medium employing anti-AVP serum which was obtained by immunizing rabbits. At a final dilution of 1/12,000, the antiserum showed less than 0.66 and 0.01% cross reactivity with lysine-vasopressin and oxytocin, respectively. But it did not cross reacted with other peptide hormones. The lowest detectable level of vasopressin was 0.5 pg/tube. The intra-assay coefficient of variation averaged 10.4%. The dilution curve of perifused medium was well paralled to the standard curve of AVP assay. AVP release from HNS or neural lobe gradually declined to the stable level in 90-120 min after the initiation of perifusion. Good repeatability of the AVP release from neural lobe was recognized by repeated stimulation with 10 min perifusion of 60 mM KCl at every 60 min. HNS released AVP in dose related manner to the osmotic challenge of sodium or glucose, and AVP release was stimulated from HNS by prostaglandin E2, but not by dopamine. These results show that the perifusion methods using AVP-RIA is a useful method to examine the AVP release from HNS or neural lobe.</p>

en-copyright=
kn-copyright=

en-aut-name=OhnoNorihito
en-aut-sei=Ohno
en-aut-mei=Norihito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HashimotoKozo
en-aut-sei=Hashimoto
en-aut-mei=Kozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YunokiSho
en-aut-sei=Yunoki
en-aut-mei=Sho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakaharaJiro
en-aut-sei=Takahara
en-aut-mei=Jiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OfujiTadashi
en-aut-sei=Ofuji
en-aut-mei=Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=35
cd-vols=
no-issue=3
article-no=
start-page=197
end-page=204
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1981
dt-pub=198106
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A scanning electron microscopic study of the two-step effect of cytochalasin B on Ehrlich ascites tumor cells.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The effect of cytochalasin B (CB) on the surface structure of Ehrlich ascites tumor cells was investigated using the scanning electron microscope. The effect occurs in two steps: formation of zeiotic knobs on the cell surface and subsequent grouping of the knobs at one pole of the cell. The early step of zeiotic knob formation occurs at low concentrations of CB (0.5-1 microgram/ml) at 37 degrees C and at high concentrations of the drug (5-10 microgram/ml) at low temperature but within 1 min at 37 degrees C. This step is only partially inhibited by 5 x 10(-3) M sodium azide. The subsequent grouping of zeiotic knobs lasts for more than 2 min at 37 degrees C and occurs only in the case of high concentrations of CB. It is inhibited by sodium azide and is often associated with grouping of the microvilli, which are then lost from all of the cell surface except the area of knob-grouping.</p>

en-copyright=
kn-copyright=

en-aut-name=SasakiJunzo
en-aut-sei=Sasaki
en-aut-mei=Junzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ImanakaMasaaki
en-aut-sei=Imanaka
en-aut-mei=Masaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WatanabeSadahiro
en-aut-sei=Watanabe
en-aut-mei=Sadahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OtsukaNagayasu
en-aut-sei=Otsuka
en-aut-mei=Nagayasu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NakamotoShu
en-aut-sei=Nakamoto
en-aut-mei=Shu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MoriMasaharu
en-aut-sei=Mori
en-aut-mei=Masaharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University
en-keyword=cytochalasin B
kn-keyword=cytochalasin B
en-keyword=Ehrlich ascites tumor cells
kn-keyword=Ehrlich ascites tumor cells
en-keyword=zeiosis
kn-keyword=zeiosis
en-keyword= scanning electron microscopy.
kn-keyword= scanning electron microscopy.
END

start-ver=1.4
cd-journal=joma
no-vol=35
cd-vols=
no-issue=5
article-no=
start-page=377
end-page=379
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1981
dt-pub=198111
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Long-term preservation of transfecting activity of avian sarcoma proviral DNA.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The integrated proviral DNA of avian sarcoma virus (ASV) in host cell chromosomes has been isolated and stored in saline sodium citrate (SSC) solution or in 70% ethanol at 4 degrees C in a refrigerator over 4 years. This DNA was assayed by transfection of chick embryo cells(CEC). The biological activity of cellular transformation by the stored DNA was compared with that of a fresh isolate of the proviral DNA. The efficiency of the transfection by each DNA was almost the same.</p>

en-copyright=
kn-copyright=

en-aut-name=OguraHajime
en-aut-sei=Ogura
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=FujiwaraTazuko
en-aut-sei=Fujiwara
en-aut-mei=Tazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University
en-keyword=avian sarcoma proviral DNA
kn-keyword=avian sarcoma proviral DNA
en-keyword=saline sodium citrate
kn-keyword=saline sodium citrate
en-keyword= transfection.
kn-keyword= transfection.
END

start-ver=1.4
cd-journal=joma
no-vol=42
cd-vols=
no-issue=6
article-no=
start-page=335
end-page=342
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=198812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of heart rate and myocardial contractile force on coronary resistance.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The effect of the heart rate and myocardial contractile force on the extravascular resistance to blood flow of the left anterior descending coronary artery (LAD) was evaluated in 15 mongrel dogs anesthetized with sodium pentobarbital. The LAD was maximally dilated by intracoronary infusion of adenosine, which precluded the influence of vasomotor tone. Increases in the heart rate and myocardial contractile force decreased coronary blood flow in the absence of a change in coronary perfusion pressure. The changes in mean coronary resistance showed a significant linear relationship to changes in developed tension. The changes in coronary resistance caused by varying the heart rate and contractile force were so small that a normal coronary vascular tree could easily compensate for the increase in resistance. However, it is supposed that with critical stenosis of the vascular tree even a small increase in resistance might cause deleterious effects on coronary blood flow.</p>

en-copyright=
kn-copyright=

en-aut-name=SaitoDaiji
en-aut-sei=Saito
en-aut-mei=Daiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=UeedaMasayuki
en-aut-sei=Ueeda
en-aut-mei=Masayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HinaKazuyoshi
en-aut-sei=Hina
en-aut-mei=Kazuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WatanabeHirofumi
en-aut-sei=Watanabe
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MimaTsutomu
en-aut-sei=Mima
en-aut-mei=Tsutomu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HasuiMasahiro
en-aut-sei=Hasui
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YamadaNobuyuki
en-aut-sei=Yamada
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=HaraokaShoichi
en-aut-sei=Haraoka
en-aut-mei=Shoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University 

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama University 

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama  University
en-keyword=contractile force
kn-keyword=contractile force
en-keyword=tachycardia
kn-keyword=tachycardia
en-keyword=extravascular resistance
kn-keyword=extravascular resistance
en-keyword=coronary flow
kn-keyword=coronary flow
en-keyword=adenosine
kn-keyword=adenosine
END

start-ver=1.4
cd-journal=joma
no-vol=62
cd-vols=
no-issue=6
article-no=
start-page=373
end-page=378
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200812
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Clinical Investigation of the Mechanism of Loxoprofen, a Non-steroidal Anti-inflammatory Drug, for Patients with Nocturia
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We previously reported the effectiveness of loxoprofen sodium (loxoprofen), a non-steroidal anti-inflammatory drug, for patients with lower urinary tract symptoms (LUTS) complaining of nocturia. In this study, we explored the mechanism of loxoprofen in the treatment of nocturia. Fifty-six patients complaining of nocturia were enrolled. They took a single 60-mg tablet of loxoprofen at bedtime for 14 days. The effects of this treatment were assessed by bladder diaries. Nocturia improved (nocturia decreased &#8805;1 void/night) in 40 patients (71.4%). Nocturnal urine volume was reduced in 31 of 40 (77.5%) without nocturnal single-void volume increase. Nocturnal single-void volume increased in 4 of 40 (10.0%) without nocturnal urine volume reduction. Two of 40 (5.0%) demonstrated both nocturnal urine volume reduction and nocturnal single-void volume increase. Three (7.5%) were exceptions to the above. In conclusion, the main mechanism of loxoprofen is the reduction of nocturnal urine volume for the treatment of nocturia and the second mechanism is the increased bladder capacity.</p>
en-copyright=
kn-copyright=

en-aut-name=ArakiTohru
en-aut-sei=Araki
en-aut-mei=Tohru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YokoyamaTeruhiko
en-aut-sei=Yokoyama
en-aut-mei=Teruhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ArakiMotoo
en-aut-sei=Araki
en-aut-mei=Motoo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FuruyaSeiji
en-aut-sei=Furuya
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Araki Urological Clinic

affil-num=2
en-affil=
kn-affil=Department of Urology, Kawasaki Medical School

affil-num=3
en-affil=
kn-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=4
en-affil=
kn-affil=Furuya Hospital
en-keyword=loxoprofen
kn-keyword=loxoprofen
en-keyword=nocturia
kn-keyword=nocturia
en-keyword=NSAID
kn-keyword=NSAID
END

start-ver=1.4
cd-journal=joma
no-vol=29
cd-vols=
no-issue=1
article-no=
start-page=29
end-page=32
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1975
dt-pub=197502
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Microheterogeneity of rat alpha-fetoprotein.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A purified and homogeneous preparation of rat alpha-fetoprotein (AFP) was separated into two components, AFPa and AFPb, by polyacrylamide gel electrophoresis. These two components had a definite difference in electrostatic net charge and gave only a single band on sodium dodecyl sulfate-electrophoresis. Neuraminidase-treated AFP gave clearly separable, slower moving four to six and finally two components depending on the time of incubation with neuraminidase. The time-dependent conversion of each AFPa and AFPb into slower migrating components upon neuraminidase treatment was confirmed by re-electrophoresis of separated and similarly treated AFPa and AFPb.</p>

en-copyright=
kn-copyright=

en-aut-name=WatanabeAkiharu
en-aut-sei=Watanabe
en-aut-mei=Akiharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TaketaKazuhisa
en-aut-sei=Taketa
en-aut-mei=Kazuhisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KosakaKiyowo
en-aut-sei=Kosaka
en-aut-mei=Kiyowo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama Univeristy
en-keyword=Microheterogeneity
kn-keyword=Microheterogeneity
en-keyword=alpha-fetoprotein
kn-keyword=alpha-fetoprotein
END

start-ver=1.4
cd-journal=joma
no-vol=29
cd-vols=
no-issue=6
article-no=
start-page=421
end-page=429
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1975
dt-pub=197512
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of sodium dodecyl sulfate on immuno-electrosyneresis between normal human erythrocyte membrane and sera of systemic lupus erythematosus patients
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>An anti-membrane antibody was present in the sera of systemic lupus erythematosus patients in immunoelectrosyneresis with sodium dodecyl sulfate (SDS) solubilized erythrocyte membrane as antigen. The SDS bound to protein was detected by chromatography at 10(-3)M concentration under U.V. light, at 10(-5)M concentration by the distilled water spray method and at 10(-6)M concentration by using rosaniline hydrochloride colorimetry. SDS was removed from the membrane protein at a concentration of 10(-3)M by the first gel filtration of Sephadex G-25 column and at a concentration of 10(-6)M by rechromatography of the same column. More than 99% of SDS in the solubilized erythrocyte membrane was removed by gel filtration. The antigenicity was still positive in the refiltrated fractions of systemic lupus erythematosus patients. Therefore, all precipitates in the gels were antigen-antibody aggregates.</p>

en-copyright=
kn-copyright=

en-aut-name=ArimoriShigeru
en-aut-sei=Arimori
en-aut-mei=Shigeru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShinozawaShinya
en-aut-sei=Shinozawa
en-aut-mei=Shinya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HirakiKiyoshi
en-aut-sei=Hiraki
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=43
cd-vols=
no-issue=2
article-no=
start-page=127
end-page=129
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198904
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Rapid purification of squirrel monkey retrovirus-H major gag protein by high performance liquid chromatography.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The major gag protein (p34) of squirrel monkey retrovirus-H was purified in one chromatographic step by anion-exchange high performance liquid chromatography. The virus in a crude fraction was disrupted with Brij 35 in the presence of three kinds of protease inhibitors. The soluble virus lysate was injected into a Polyanion SI column, and p34 was eluted with a linear salt gradient. The recovery of the protein was about 60%. The purified p34 was nearly homogenous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining.</p>

en-copyright=
kn-copyright=

en-aut-name=IkedaShogo
en-aut-sei=Ikeda
en-aut-mei=Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsutsuiKen
en-aut-sei=Tsutsui
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HatsushikaMasao
en-aut-sei=Hatsushika
en-aut-mei=Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WatanabeSekiko
en-aut-sei=Watanabe
en-aut-mei=Sekiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OdaTakuzo
en-aut-sei=Oda
en-aut-mei=Takuzo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama  University

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama  University
en-keyword=retrovirus
kn-keyword=retrovirus
en-keyword=gag protein
kn-keyword=gag protein
en-keyword=protein purification
kn-keyword=protein purification
en-keyword=high performance liquid chromatography
kn-keyword=high performance liquid chromatography
END

start-ver=1.4
cd-journal=joma
no-vol=51
cd-vols=
no-issue=4
article-no=
start-page=195
end-page=206
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1997
dt-pub=199708
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=cDNA cloning, sequence analysis and expression of a mouse 44-kDa nuclear protein copurified with DNA repair factors for acid-depurinated DNA
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We purified a 44-kDa nuclear protein from salt-extract of permeable mouse ascites sarcoma cells in an effort to isolate factors involved in the repair of acid-depurinated DNA. It was copurified with a major AP endonuclease (APEX nuclease) by sequential column chromatography then further purified by sodium dodecyl sulphate-poly-acrylamide gel electrophoresis as a possible DNA repair support factor. Its partial amino acid sequences were determined, and a cDNA clone for the protein was isolated from a mouse T-cell cDNA library using long degenerate oligonucleotide probes deduced from the amino acid sequence. The complete nucleotide sequence of the cDNA (1.7 kilobases) was determined. Northern hybridization using this cDNA detected two transcripts: 1.8kb being the major one and 2.6 kb being the minor one. The complete amino acid sequence for the protein predicted from the nucleotide sequence of the cDNA indicates that the 44-kDa protein consists of 394 amino acids with a calculated molecular weight of 43,698. In tests performed thus far, the recombinant 44-kDa protein expressed in Escherichia coli has not expressed any repair-support activity. It remains to be analyzed whether the protein attains this activity after appropriate posttranslational modifications. Most parts of the 44-kDa protein cDNA and the deduced amino acid sequence were found to be identical to those of the protein p38 -2G4, recently reported as a cell cycle-specifically modulated nuclear protein of 38kDa. The p38-2G4 may be a truncated form of the present 44-kDa protein.</p>

en-copyright=
kn-copyright=

en-aut-name=NakagawaYuko
en-aut-sei=Nakagawa
en-aut-mei=Yuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WatanabeSekiko
en-aut-sei=Watanabe
en-aut-mei=Sekiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AkiyamaKosuke
en-aut-sei=Akiyama
en-aut-mei=Kosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SarkerAltaf H
en-aut-sei=Sarker
en-aut-mei=Altaf H
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TsutsuiKen
en-aut-sei=Tsutsui
en-aut-mei=Ken
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=InoueHajime
en-aut-sei=Inoue
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SekiShuji
en-aut-sei=Seki
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University
en-keyword=44-kDa protein
kn-keyword=44-kDa protein
en-keyword=nuclear protein
kn-keyword=nuclear protein
en-keyword=cDNA cloning
kn-keyword=cDNA cloning
en-keyword=cDNA sequencing
kn-keyword=cDNA sequencing
en-keyword=recombinant protein
kn-keyword=recombinant protein
END

start-ver=1.4
cd-journal=joma
no-vol=34
cd-vols=
no-issue=2
article-no=
start-page=131
end-page=138
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1980
dt-pub=198004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Detection and characterization of circulating immune complexes during acute exacerbation of chronic viral hepatitis.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>For the detection and characterization of circulating immune complexes (CIC) in various liver diseases, a Clq binding test was used. Though the CIC level was almost normal in HB surface antigen (HBsAg) positive asymptomatic carriers, the level increased in patients with liver diseases. During acute exacerbation of chronic viral hepatitis, the CIC level reached peaks 1 to 3 weeks before and after the hepatic cell necrosis. Study of the sedimentation rates of CIC in various liver diseases showed CIC in the 19s-22s region and in the 7s-19s region. In acid buffer, CIC was dissociated into 5 to 6 components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In one case of HBsAg positive severe chronic aggressive hepatitis, CIC was composed of HBsAg, IgG and another three or four undetermined components. During acute exacerbation of chronic hepatitis, minor changes of these dissociation patterns of CIC were observed.</p>

en-copyright=
kn-copyright=

en-aut-name=ArakiKiyonori
en-aut-sei=Araki
en-aut-mei=Kiyonori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OnoueKimiaki
en-aut-sei=Onoue
en-aut-mei=Kimiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TuchiyaMasao
en-aut-sei=Tuchiya
en-aut-mei=Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=ShinoharaToru
en-aut-sei=Shinohara
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=InoueJunichi
en-aut-sei=Inoue
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NagashimaHideo
en-aut-sei=Nagashima
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University

affil-num=2
en-affil=
kn-affil=Okayama University

affil-num=3
en-affil=
kn-affil=Okayama University

affil-num=4
en-affil=
kn-affil=Okayama University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University
en-keyword=chronic viral hepatitis
kn-keyword=chronic viral hepatitis
en-keyword=circulating immune complexes
kn-keyword=circulating immune complexes
en-keyword=hepatic cell necrosis
kn-keyword=hepatic cell necrosis
en-keyword=HB surface antigen
kn-keyword=HB surface antigen
en-keyword= GIq.
kn-keyword= GIq.
END

start-ver=1.4
cd-journal=joma
no-vol=50
cd-vols=
no-issue=3
article-no=
start-page=139
end-page=144
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=199606
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Relationship between Response to Interferon Therapy and Detection of Hepatitis C Virus RNA by Differential Flotation Centrifugation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>We purified an apurinic/apyrimidinic (AP) endonuclease from mouse ascites sarcoma (SR-C3H/He) cells. The enzyme showed nicking activity on acid-depurinated DNA but not on untreated, intact DNA. It also showed priming activity for DNA polymerase on both acid-depurinated and bleomycin-damaged DNA. The priming activity on bleomycin-damaged DNA was two times higher than that on an acid-depurinated DNA. The enzymatic properties indicate that the enzyme is a class II AP endonuclease having DNA 3' repair diesterase activity. The purified enzyme has a molecular weight of 39,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimal pH for AP endonuclease activity was 8.0 in 50 mM Tris-HCl buffer. The AP endonuclease activity depended on divalent cation such as Mg2+ and Co2+ ions, and was inhibited by 2 mM EDTA with no addition of the divalent cation. An appropriate concentration of sodium or potassium salt stimulated the activity. Partial digestion of the AP endonuclease with Staphylococcus aureus V8 protease produced 4 major peptide fragments which may be used for protein sequencing.</p>

en-copyright=
kn-copyright=

en-aut-name=WatoMasaki
en-aut-sei=Wato
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ShimomuraHiroyuki
en-aut-sei=Shimomura
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FujioKozo
en-aut-sei=Fujio
en-aut-mei=Kozo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TsujiHideyuki
en-aut-sei=Tsuji
en-aut-mei=Hideyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KondoJunichi
en-aut-sei=Kondo
en-aut-mei=Junichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=FujiokaShin-ichi
en-aut-sei=Fujioka
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=IshiiYasushi
en-aut-sei=Ishii
en-aut-mei=Yasushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=HadaHajime
en-aut-sei=Hada
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama Univeristy

affil-num=4
en-affil=
kn-affil=Okayama  University

affil-num=5
en-affil=
kn-affil=Okayama University

affil-num=6
en-affil=
kn-affil=Okayama University

affil-num=7
en-affil=
kn-affil=Okayama University 

affil-num=8
en-affil=
kn-affil=Okayama University

affil-num=9
en-affil=
kn-affil=Okayama University
en-keyword=hepatitis C
kn-keyword=hepatitis C
en-keyword=ultracentrifugation
kn-keyword=ultracentrifugation
en-keyword=immune complex
kn-keyword=immune complex
en-keyword=interferon
kn-keyword=interferon
END

start-ver=1.4
cd-journal=joma
no-vol=50
cd-vols=
no-issue=3
article-no=
start-page=131
end-page=137
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=199606
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Purification and characterization of a 39kDa apurinic/apyrimidinic endonuclease from mouse ascites sarcoma cells.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>&#60;P&#62;We purified an apurinic/apyrimidinic (AP) endonuclease from mouse ascites sarcoma (SR-C3H/He) cells. The enzyme showed nicking activity on acid-depurinated DNA but not on untreated, intact DNA. It also showed priming activity for DNA polymerase on both acid-depurinated and bleomycin-damaged DNA. The priming activity on bleomycin-damaged DNA was two times higher than that on an acid-depurinated DNA. The enzymatic properties indicate that the enzyme is a class II AP endonuclease having DNA 3' repair diesterase activity. The purified enzyme has a molecular weight of 39,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimal pH for AP endonuclease activity was 8.0 in 50 mM Tris-HCl buffer. The AP endonuclease activity depended on divalent cation such as Mg2+ and Co2+ ions, and was inhibited by 2 mM EDTA with no addition of the divalent cation. An appropriate concentration of sodium or potassium salt stimulated the activity. Partial digestion of the AP endonuclease with Staphylococcus aureus V8 protease produced 4 major peptide fragments which may be used for protein sequencing.&#60;/P&#62;</p>

en-copyright=
kn-copyright=

en-aut-name=WakabayashiHajime
en-aut-sei=Wakabayashi
en-aut-mei=Hajime
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SekiShuji
en-aut-sei=Seki
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama Univeristy
en-keyword=AP endonuclease
kn-keyword=AP endonuclease
en-keyword=DNA 3' repair diesterase
kn-keyword=DNA 3' repair diesterase
en-keyword=DNA repair enzyme
kn-keyword=DNA repair enzyme
en-keyword=mouse ascites sarcoma cells
kn-keyword=mouse ascites sarcoma cells
END

start-ver=1.4
cd-journal=joma
no-vol=44
cd-vols=
no-issue=1
article-no=
start-page=47
end-page=50
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1990
dt-pub=199002
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Determination of hypotaurine and taurine in blood plasma of rats after the administration of L-cysteine.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>A method for the simultaneous determination of hypotaurine and taurine was developed. The method consisted of the elimination of urea, which interfered with the determination of hypotaurine, by immobilized urease, and determination of hypotaurine and taurine with an amino acid analyzer. The analyzer equipped with a cation-exchange column was operated at 32 degrees C with 0.2 M sodium citrate buffer, pH 2.8. Using this method, the dynamics of hypotaurine and taurine in blood plasma of rats was studied after the intraperitoneal injection of L-cysteine. The concentration of cysteine reached the maximum 1 h after L-cysteine loading. The concentration of hypotaurine and taurine increased in parallel and reached the maximum 2 h after L-cysteine loading. These changes seem to indicate the precursor-product relationship of these substances and the rapid conversion of hypotaurine to taurine in vivo.</p>

en-copyright=
kn-copyright=

en-aut-name=YuasaShigeki
en-aut-sei=Yuasa
en-aut-mei=Shigeki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AkagiReiko
en-aut-sei=Akagi
en-aut-mei=Reiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UbukaToshihiko
en-aut-sei=Ubuka
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama  University

affil-num=2
en-affil=
kn-affil=Okayama  University

affil-num=3
en-affil=
kn-affil=Okayama University
en-keyword=hypotaurine
kn-keyword=hypotaurine
en-keyword=taurine
kn-keyword=taurine
en-keyword=determination
kn-keyword=determination
en-keyword=cysteine metabolisn
kn-keyword=cysteine metabolisn
en-keyword=amino acid analysis
kn-keyword=amino acid analysis
END

start-ver=1.4
cd-journal=joma
no-vol=49
cd-vols=
no-issue=5
article-no=
start-page=231
end-page=236
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1995
dt-pub=199510
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Possible Roles of Nitric Oxide and Vasoactive Intestinal Polypeptide on Relaxation Induced by Isoprenaline in Isolated Muscle Strips of the Mouse Gastric Fundus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The possible role of nitric oxide (NO) and vasoactive intestinal polypeptide on isoprenaline-induced relaxation of the mouse longitudinal gastric fundal strips precontracted with 5.4 x 10(-7) M carbachol was investigated. Isoprenaline (5 x 10(-7) M, 10(-6) M and 5 x 10(-6) M) produced a concentration-dependent relaxations. NG-nitro L-arginine (10(-4) M) partly inhibited isoprenaline-induced relaxation. The inhibitory action of NG-nitro L-arginine was reversed by 4 x 10(-4) M L-arginine but not by 4 x 10(-4) M D-arginine. NG-nitro L-arginine (10(-4) M) did not affect the relaxation caused by sodium nitroprusside (10(-6) M). Vasoactive intestinal polypeptide antibody 7913 (1:160 dilution) partly inhibited isoprenaline-induced relaxation. This inhibition was greater on the response to the higher isoprenaline concentration (5 x 10(-6) M) than to the lower concentration (10(-6) M). The combination of vasoactive intestinal polypeptide antibody and NG-nitro L-arginine significantly enhanced the inhibition on 10(-6) M isoprenaline action. These results suggest that nitric oxide and vasoactive intestinal polypeptide may partly contribute to the relaxation induced by isoprenaline in the mouse gastric fundus precontracted with carbachol.</p>

en-copyright=
kn-copyright=

en-aut-name=OgulenerNuran
en-aut-sei=Ogulener
en-aut-mei=Nuran
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=LarabalEda
en-aut-sei=Larabal
en-aut-mei=Eda
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=BaysalFiruz
en-aut-sei=Baysal
en-aut-mei=Firuz
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=DikmenAtilla
en-aut-sei=Dikmen
en-aut-mei=Atilla
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Cukurova University

affil-num=2
en-affil=
kn-affil=Cukurova University

affil-num=3
en-affil=
kn-affil=Cukurova University

affil-num=4
en-affil=
kn-affil=Cukurova University
en-keyword=isoprenaline
kn-keyword=isoprenaline
en-keyword= N<sub>G<sub>-nitro L-arginine(L-NOARG)
kn-keyword= N<sub>G<sub>-nitro L-arginine(L-NOARG)
en-keyword= L-arginine(L-ARG)
kn-keyword= L-arginine(L-ARG)
en-keyword= D-arginine(D-ARG)
kn-keyword= D-arginine(D-ARG)
en-keyword=vasoactive intestinal polypeptide (VIP) antibody 7913
kn-keyword=vasoactive intestinal polypeptide (VIP) antibody 7913
en-keyword=isolated mouse gastric fundus
kn-keyword=isolated mouse gastric fundus
END

start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=3
article-no=
start-page=164
end-page=172
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1956
dt-pub=195607
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The effect of an antihistamine agent on the gastric secretion induced by sinomenine and irgapyrin
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>1. Sinomenine and Irgapyrin, the two antirheumatics
known to be capable of releasing histamine, caused a marked
gastric secretion in the unanesthetized dog. 2. The facial edema and itching associated with histamine release by sinomenine was almost completely eliminated by NeoAntergan,
but the gastric secretion was not suppressed, or rather increased - an observation also reported by Paton and
Schachter with Compound 48/80. This indicates that the histamine release cannot be markedly prevented by antihistamine agents in this animal. 3. The gastric secretion induced by Irgapyrin was not suppressed
by Neo-Antergan but Irgapyrin originally never caused
other symptoms associated with histamine release. This is probably due to the antihistamine action inherent in this compound itself. 4. No such histamine-releasing activity, as determined by gastric secretion, could be observed in aminopyrine or butazolidine sodium, the components of Irgapyrin. 5. Sinomenine, differing from Irgapyrin and Compound 48/80, was ineffective by intramuscular injection.</p>

en-copyright=
kn-copyright=

en-aut-name=NishiyamaRyosaku
en-aut-sei=Nishiyama
en-aut-mei=Ryosaku
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=53
cd-vols=
no-issue=
article-no=
start-page=67
end-page=76
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1983
dt-pub=19830325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the Munyeijin potassium salt deposits in Yunnan Province, People's Republic of China
kn-title=雲南省勐 野井カリ塩鉱床の古水文地質について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In Langping-Simao basins of Southwestern Yunnan Province, a number of salt deposits and salt springs are distributed (Fig. 1). The salt-bearing formations are sandstones, silt and mudstones of the Cretaceous to Paleogene ages, although the ages of the potassium-salts deposits seem to be younger. The Munyeijin potassium deposit was found on the eastern side of the Simao basin in early nineteen sixties. Although the salt deposit has been strongly deformed by tectonic movements (Fig. 2), the deposit has salt-dome structure and three depositional stages have been identified in each cycle of depositional sequence. They are, from the bottom to the top, red salts, black and white salts and carnallite-bearing clayey rocks (see Fig. 3). The red colour of the bottom zone is due to globular debris of silt in the red salts, whereas the top clayey rocks are high in organic materials, being characteristic of the residue of the last stage evaporation of a salt lake. Potassium salt is found in all the three zones, filling up the grain gaps of sodium chloride crystals or running through salt beds in veins and veinlets, although the black-white salts are the most fertile in potassium and have been mined for commercial use. Small amounts of carbonates and sulfates (gypsum and anhydrite) also exist as fine, dispersed grains. Thin layers of gypsum are not uncommon in dark portions of the red and black-white salts. Tachhydrite (CaCl(2)・2MgCl(2)・6H(2)O) is also observed in the top clayey deposits. From the field observation, geological, mineralogical, and geochemical considerations, the paleohydrogeological environments which led to the formation of potassium-rich salt deposits at Munyeijin are reconstructed as follows: 1) The depositional stage of the carnallite-bearing sediments: This is the last stage of evaporation of a salt lake. The sediments would have had up to 80 vol. % interstitial waters saturated with carnallite and sylvite. 2) Diagenetic stage: The carnallite-clay deposits were covered by younger salt deposits and the pore water was gradually squeezed out by compression. The pore water penetrated into the underlying sodium chloride deposits, where the pore water precipitated sylvite as the sodium
salts were essentially devoid of potassium. Because the pore water contained organic materials (Table 2), the sylvite precipitates were dark in colour. 3) Tectonic stage: The Langping and Simao basins are lined along the Sanjian tectonic belts. The tectonic activity which presumably started in Eocene must be responsible for the intense deformation of the salt beds. Enrichment of potassium along the axis of folding and the anhydrite formation with the axis of crystallization tilted to the plane of salt beds are some of the important paleohydrogeological results of such movements. Several lines of evidence strongly suggest that hydrothermal activity took place widely in Munyejin basin during this stage. The origin of the potassium-bearing brines has been debated in China since the discovery of the deposits. Many lines of evidence suggest it be of marine origin. However, the presence of tachhydrite in the carnallite-bearing clayey deposits requires some additional source(s) of calcium in addition to seawater. The highly saline groundwaters in Triassic through Jurassic formations
of Sichuan Province often are rich in Ca(2+) as well as Mg(2+) and K(+) as some examples are shown in Table 3. If such saline ground waters flew into a salt lake and was subjected to evaporation, calcium-bearing salts such as tachhydrite may form at the last stage deposit of the lake. The origin of such groundwaters is an interesting
problem to be studied in future.
en-copyright=
kn-copyright=

en-aut-name=JiaShuyuan
en-aut-sei=Jia
en-aut-mei=Shuyuan
kn-aut-name=賈疏源
kn-aut-sei=賈
kn-aut-mei=疏源
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=成都地質学院水文地質工程地質学部
END

start-ver=1.4
cd-journal=joma
no-vol=54
cd-vols=
no-issue=
article-no=
start-page=1
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1984
dt-pub=19840325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Research for carbon dioxide bathing IV, Thermal effect of artificial CO(2)-bathing
kn-title=人工炭酸浴に関する研究 第4報, 人工炭酸浴の効果
en-subtitle=
kn-subtitle=
en-abstract=The artificial CO(2)-bath was prepared with a tablet (50g), made from sodium bicarbonate and succinic acid, putting simply in plain water bathtub of 150-200 litre at 40℃. Thermal effect was evaluated by means of thermography in healthy 8 men; temperature areas of 34.5℃ or greater were recorded in average compared to a plain bathing and to an artificial Na(2)SO(4)-NaHCO(3) one 10 minutes later. Clinical evaluation by questionnairings of 24 patients suffering mainly from lumbago and 4-extremity coldness revealed a long lasting peripheral warmth in 90% and the ease from pain in 85%. The artificial CO(2)-bathing was effective for women whose health were adversely affected
by the cold in 63.6% and for general fatigue or dullness in 56.5% of 664 female volunteers whom the questionnairings were conducted to. No side effect was encountered in the survey.
kn-abstract=1) 炭酸塩と,コ-ク酸からなる錠剤型の｢炭酸ガス浴剤｣の保温作用を健康な男子8名について,サ-モグラフィーを用いて測定した. 入浴10分後の比較で明らかに,炭酸ガス浴はよく温まった結果,表面温度が高くなっている. 2) 腰痛,四肢冷感,その他の患者24名での臨床評価の結果,患者の90%以上が手足が温まり,湯ざめしにくいことを認めた.また,患者の85%は痛みがやわらぐことを認めた. 3) 主婦664名を対象とした使用評価の結果,常時手足の冷感を訴える者の63.6%,身体の疲労感･だるさ56.5%に効果を認めていることがわかった. 4) 副作用は全く認められなかった.
en-copyright=
kn-copyright=

en-aut-name=YorozuHidenori
en-aut-sei=Yorozu
en-aut-mei=Hidenori
kn-aut-name=萬秀憲
kn-aut-sei=萬
kn-aut-mei=秀憲
aut-affil-num=1
ORCID=

en-aut-name=KuboYuichiro
en-aut-sei=Kubo
en-aut-mei=Yuichiro
kn-aut-name=久保裕一郎
kn-aut-sei=久保
kn-aut-mei=裕一郎
aut-affil-num=2
ORCID=

en-aut-name=EguchiYasuteru
en-aut-sei=Eguchi
en-aut-mei=Yasuteru
kn-aut-name=江口泰輝
kn-aut-sei=江口
kn-aut-mei=泰輝
aut-affil-num=3
ORCID=

en-aut-name=SunakawaMitsuru
en-aut-sei=Sunakawa
en-aut-mei=Mitsuru
kn-aut-name=砂川満
kn-aut-sei=砂川
kn-aut-mei=満
aut-affil-num=4
ORCID=

en-aut-name=KohmotoTomoji
en-aut-sei=Kohmoto
en-aut-mei=Tomoji
kn-aut-name=河本知二
kn-aut-sei=河本
kn-aut-mei=知二
aut-affil-num=5
ORCID=

en-aut-name=KomotoYoshiaki
en-aut-sei=Komoto
en-aut-mei=Yoshiaki
kn-aut-name=古元嘉昭
kn-aut-sei=古元
kn-aut-mei=嘉昭
aut-affil-num=6
ORCID=

en-aut-name=Komoto]unko
en-aut-sei=Komoto
en-aut-mei=]unko
kn-aut-name=古元順子
kn-aut-sei=古元
kn-aut-mei=順子
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=花王石鹸株式会社栃木研究所

affil-num=2
en-affil=
kn-affil=花王石鹸株式会社栃木研究所

affil-num=3
en-affil=
kn-affil=花王石鹸株式会社栃木研究所

affil-num=4
en-affil=
kn-affil=岡山大学温泉研究所リハビリテーション医学部門

affil-num=5
en-affil=
kn-affil=岡山大学温泉研究所リハビリテーション医学部門

affil-num=6
en-affil=
kn-affil=岡山大学温泉研究所リハビリテーション医学部門

affil-num=7
en-affil=
kn-affil=岡山大学医学部附属病院三朝分院リハビリテーション部
END

start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=
article-no=
start-page=9
end-page=11
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1985
dt-pub=19850330
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=An application of the artificial CO(2) bath for enuresis
kn-title=夜尿症に対する人工炭酸泉浴の試み
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The artificial CO(2) bath was prescribed for two enuretic boys with bladder-sensitive or parasympaticotonic type. A tablet of 50 g, made from sodium bicarbonate and succinic acid, was put in a bath-tub of 150-200 L at 40℃. Each patient was recommended to take a bath with his mother longer than ten minutes, and to mark the presence or the absence of enuresis every day. Outcome results of case 1 on CO(2) bath is shown in tabel 1 and results of case 2 on CO(2) bath is shown in table 3. Both two cases became free from enuresis 7-10 days after the initiation of the artificial CO(2) bathing. Etiological mechanisms of enuresis seem to be multi-factorious, with underlining networks of central- and autonomous nerve systems for the control of sleep and bladder. It is suspected that some improvement of peripheral circulation by CO(2)-bathing leading to keep the body warm may be one of the contributing factors which prolong the intervals of urination, resulting in autonomous normalization, at least, for the cases of bladder-sensitive enuresis, Further case studies are needed to support this assumption.
en-copyright=
kn-copyright=

en-aut-name=KomotoJunko
en-aut-sei=Komoto
en-aut-mei=Junko
kn-aut-name=古元順子
kn-aut-sei=古元
kn-aut-mei=順子
aut-affil-num=1
ORCID=

en-aut-name=KomotoYoshiaki
en-aut-sei=Komoto
en-aut-mei=Yoshiaki
kn-aut-name=古元嘉昭
kn-aut-sei=古元
kn-aut-mei=嘉昭
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部附属三朝分院リハビリテーション部

affil-num=2
en-affil=
kn-affil=岡山大学医学部附属三朝分院リハビリテーション部
END

start-ver=1.4
cd-journal=joma
no-vol=122
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=7
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Collectrin is involved in the development of salt-sensitive hypertension by facilitating the membrane trafficking of apical membrane proteins via interaction with soluble n-ethylmaleiamide-sensitive factor attachment protein receptor complex
kn-title=コレクトリンはSNARE複合体との相互作用を介して管腔側膜蛋白の膜輸送を促進し食塩感受性高血圧の発症に関与している
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=YasuharaAkihiro
en-aut-sei=Yasuhara
en-aut-mei=Akihiro
kn-aut-name=安原章浩
kn-aut-sei=安原
kn-aut-mei=章浩
aut-affil-num=1
ORCID=

en-aut-name=WadaJun
en-aut-sei=Wada
en-aut-mei=Jun
kn-aut-name=和田淳
kn-aut-sei=和田
kn-aut-mei=淳
aut-affil-num=2
ORCID=

en-aut-name=EguchiJun
en-aut-sei=Eguchi
en-aut-mei=Jun
kn-aut-name=江口潤
kn-aut-sei=江口
kn-aut-mei=潤
aut-affil-num=3
ORCID=

en-aut-name=NakatsukaAtsuko
en-aut-sei=Nakatsuka
en-aut-mei=Atsuko
kn-aut-name=中司敦子
kn-aut-sei=中司
kn-aut-mei=敦子
aut-affil-num=4
ORCID=

en-aut-name=MurakamiKazutoshi
en-aut-sei=Murakami
en-aut-mei=Kazutoshi
kn-aut-name=村上和敏
kn-aut-sei=村上
kn-aut-mei=和敏
aut-affil-num=5
ORCID=

en-aut-name=KanzakiMotoko
en-aut-sei=Kanzaki
en-aut-mei=Motoko
kn-aut-name=神崎資子
kn-aut-sei=神崎
kn-aut-mei=資子
aut-affil-num=6
ORCID=

en-aut-name=TeshigawaraSanae
en-aut-sei=Teshigawara
en-aut-mei=Sanae
kn-aut-name=勅使川原早苗
kn-aut-sei=勅使川原
kn-aut-mei=早苗
aut-affil-num=7
ORCID=

en-aut-name=MakinoHirofumi
en-aut-sei=Makino
en-aut-mei=Hirofumi
kn-aut-name=槇野博史
kn-aut-sei=槇野
kn-aut-mei=博史
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学

affil-num=2
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学

affil-num=5
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学

affil-num=6
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学

affil-num=7
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学

affil-num=8
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学
en-keyword=食塩感受性高血圧
kn-keyword=食塩感受性高血圧
en-keyword=コレクトリン
kn-keyword=コレクトリン
en-keyword=集合管
kn-keyword=集合管
en-keyword=SNARE複合体
kn-keyword=SNARE複合体
en-keyword=ナトリウム再吸収
kn-keyword=ナトリウム再吸収
END

start-ver=1.4
cd-journal=joma
no-vol=3
cd-vols=
no-issue=3
article-no=
start-page=265
end-page=269
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1987
dt-pub=19870610
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Spectrophotometric Determination of Anionic Surfactants in River Water with Cationic AZO Dye by Solvent Extraction- Flow Injection Analysis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Anionic surfactants in water were determined by a spectrophotometric flow injection technique coupled with solvent extraction. The ion associate which formed between an anionic surfactant and an cationic azo dye was extracted into an organic solvent and the absorbance was measured. The carrier was distilled water, and the reagent solution contained an cationic azo dye and sodium sulfate, the pH of which being adjusted to 5 with acetate buffer. A phase separator with a poly(tetrafluoroethylene) porous membrane (0.8μm pore size) was used to separate the organic phase. Six derivatives of cationic azo dyes and several extracting solvents were examined; a pair of 1-methyl-4-(4-diethylaminophenylazo)- pyridinium cation and chloroform turned out best. The sampling rate was 30 samples per hour. Calibration graphs were linear up to 2×10(-6)M or 3×10(-5)M of anionic surfactant when injection volume was 300 or 100μl, respectively. The relative standard deviation(n=10) was 1.5% for 300μl of 1×10(-6)M sodium dodecylsulfate. The detection limit was as little as 1×10(-8)M of anionic surfactant. Anionic surfactants in river water were determined satisfactorily.
en-copyright=
kn-copyright=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=1
ORCID=

en-aut-name=HazakiYoshito
en-aut-sei=Hazaki
en-aut-mei=Yoshito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OshimaMitsuko
en-aut-sei=Oshima
en-aut-mei=Mitsuko
kn-aut-name=大島光子
kn-aut-sei=大島
kn-aut-mei=光子
aut-affil-num=3
ORCID=

en-aut-name=ToeiKyoji
en-aut-sei=Toei
en-aut-mei=Kyoji
kn-aut-name=桐栄恭二
kn-aut-sei=桐栄
kn-aut-mei=恭二
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学
en-keyword=Anionic surfactant determination
kn-keyword=Anionic surfactant determination
en-keyword=cationic azo dye
kn-keyword=cationic azo dye
en-keyword=ion associate extraction
kn-keyword=ion associate extraction
en-keyword=spectrophotometry
kn-keyword=spectrophotometry
en-keyword=flow injection analysis
kn-keyword=flow injection analysis
END

start-ver=1.4
cd-journal=joma
no-vol=4
cd-vols=
no-issue=1
article-no=
start-page=81
end-page=85
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=19880210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Simultaneous Determination of Sodium and Potassium Ions in River Water by Ion Chromatography Using Silica Gels as a Stationary Phase
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Alkali metal cations were shown to be separable on a silica gel column by cation exchange. Factors affecting the separation of these cations were investigated. Among them, type and concentration of the mobile phase electrolytes profoundly affected the retention of the analyte. The alkali metal cations were well separated on a 150 mm long Zorbax SIL column with 2.1mm i.d., using an aqueous 0.01mol dm(-3) lithium acetate as the mobile phase. Sodium and potassium in river water were determined rapidly with satisfactory accuracy.
en-copyright=
kn-copyright=

en-aut-name=IwachidoTadashi
en-aut-sei=Iwachido
en-aut-mei=Tadashi
kn-aut-name=岩知道正
kn-aut-sei=岩知道
kn-aut-mei=正
aut-affil-num=1
ORCID=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=IshimaruKenji
kn-aut-sei=Ishimaru
kn-aut-mei=Kenji
aut-affil-num=2
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=Alkali metals
kn-keyword=Alkali metals
en-keyword=silica gel column
kn-keyword=silica gel column
en-keyword=simultaneous determination
kn-keyword=simultaneous determination
en-keyword=sodium
kn-keyword=sodium
en-keyword=potassium
kn-keyword=potassium
en-keyword=river water
kn-keyword=river water
en-keyword=ion chromatography
kn-keyword=ion chromatography
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=4
article-no=
start-page=575
end-page=579
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19960810
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Separation and Direct Photometric Determination of Inorganic Anions by Capillary Zone Electrophoresis Using Suppressed Electroosmosis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A simple, sensitive and separative method for the photometric determination of inorganic anions was developed on the basis of suppressed electroosmosis by using a common silica capillary and a simple migrating solution. During the analysis of analyte anions by capillary zone electrophoresis, electroosmotic flow in a silica capillary was suppressed by using low-pH migrating solutions containing sodium sulfate. The stacking effect of sulfate ion was utilized for analyte concentration. Four kinds of inorganic analyte anions examined were detected in sharp signals, and the separation of a nitrate and a nitrite ion was improved by using the low-pH migrating solution with no decrease in detection sensitivity. Calibration graphs for nitrate and nitrite ions showed good linearity in the concentration ranges from about 10(-5) to 10(-4)mol dm(-3), with the detection limit for nitrate ion 4×10(-6)mol dm(-3). Separations of organic anions, such as aromatic sulfonate and carboxylate ions, were also examined; many of them were well separated by the proposed migrating solution. Those organic anions did not interfere with the determination of the inorganic anions.
en-copyright=
kn-copyright=

en-aut-name=TakayanagiToshio
en-aut-sei=Takayanagi
en-aut-mei=Toshio
kn-aut-name=高柳俊夫
kn-aut-sei=高柳
kn-aut-mei=俊夫
aut-affil-num=1
ORCID=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=WadaEiko
kn-aut-sei=Wada
kn-aut-mei=Eiko
aut-affil-num=2
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=Capillary zone electrophoresis
kn-keyword=Capillary zone electrophoresis
en-keyword=inorganic anion
kn-keyword=inorganic anion
en-keyword=direct photometric detection
kn-keyword=direct photometric detection
en-keyword=suppressed electroosmosis
kn-keyword=suppressed electroosmosis
en-keyword=stacking effect
kn-keyword=stacking effect
END

start-ver=1.4
cd-journal=joma
no-vol=36
cd-vols=
no-issue=8
article-no=
start-page=503
end-page=507
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1987
dt-pub=19870805
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Determination of total carbonate-carbon in river water by ion chromatography with photometric detection
kn-title=吸光光度検出イオンクロマトグラフィーによる河川水中の総炭酸の定量
en-subtitle=
kn-subtitle=
en-abstract=Determination of total carbonate-carbon in river water was achieved by ion chromatography with an ultraviolet photometric detector and an anion exchange column(TSK gel IC-Anion PW, 4.6 mm i.d.×50 mm). Trimellitate (1,2,4-benzenetricarboxylate, 4×10(-4) M) solution(pH 7.5) was used as an eluent. Ten microliters of sample solution was injected and decrease in absorbance of the trimellitate eluent at 270 nm was detected. Calcium and magnesium ions interfere with the determination of total carbonate-carbon. These could be removed completely by passing the sample solution through a cation exchange-type pretreatment cartridge column (sodium type). The expelled trimellitate ion from the column by anions (chloride, sulfate and nitrate ions) in sample solution decreased the peak height of hydrogen carbonate ion. This interference could be corrected by using the summation of peak areas of coexisting anions. By the proposed method, total carbonate-carbon in river water was determined. For total carbonate-carbon, the results were in good agreement with the values obtained by FIA.
kn-abstract=吸光光度検出器を用いたイオンクロマトグラフィーによる,河川水中の総炭酸(炭酸,炭酸水素イオン及び炭酸イオンの合量)の定量法が検討された.吸収を示す溶離イオンとしては,トリメリット酸イオン(1,2,4-ベンゼントリカルボン酸イオン)を用い,陰イオン交換カラム(TSK gel IC-Anion-PW, 4.6mm i.d.×50mm)を用いた.試料中のカルシウム,マグネシウムイオンは,溶離イオンのカルボン酸イオンと反応し,総炭酸の定量に影響を与える.この妨害は,Na型に変えたイオン交換型試料前処理カートリッジカラム(Toyopak IC-SPM)に試料を通すことにより完全に除くことができた.試料中の陰イオンにより,追い出されてきたトリメリテートイオンの吸収は炭酸水素イオンのピークに影響を与え,負の誤差を生じる.この誤差は,試料中に共存する陰イオンのピークの面積の和を用いることにより補正可能であることが分かった.河川水中の総炭酸の定量を行ったところ,FIAにより得られた値と良く一致した.
en-copyright=
kn-copyright=

en-aut-name=HironakaTakashi
en-aut-sei=Hironaka
en-aut-mei=Takashi
kn-aut-name=弘中孝志
kn-aut-sei=弘中
kn-aut-mei=孝志
aut-affil-num=1
ORCID=

en-aut-name=OshimaMitsuko
en-aut-sei=Oshima
en-aut-mei=Mitsuko
kn-aut-name=大島光子
kn-aut-sei=大島
kn-aut-mei=光子
aut-affil-num=2
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=indirect photometric ion chromatography
kn-keyword=indirect photometric ion chromatography
en-keyword=hydrogen carbonate ion in river water
kn-keyword=hydrogen carbonate ion in river water
en-keyword=elution with 1, 2
kn-keyword=elution with 1, 2
END

start-ver=1.4
cd-journal=joma
no-vol=37
cd-vols=
no-issue=12
article-no=
start-page=642
end-page=647
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1988
dt-pub=19881205
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Spectrophotometric determination of sodium and potassium by FIA coupled with separation on a silica column and solvent extraction
kn-title=カラム分離/溶媒抽出フローインジェクション分析法によるナトリウムとカリウムの吸光光度法
en-subtitle=
kn-subtitle=
en-abstract=Sodium and potassium ions were spectrophotometrically determined by solvent extraction flow injection incorporated with a silica gel column. The ion association complexes which formed between alkali metal-crown ether complexes and an anionic dye were extracted into an organic phase and the absorbance of the organic phase was measured after the phase separation by a phase separator with a poly(tetrafluoroethylene) porous membrane (pore size: 0.8 μm). Sodium and potassium were separated on a silica gel column (1 mm i.d.×20 cm; 100200 mesh silica gel). Four streams, a carrier, an eluent, a reagent solution and an extraction solvent, were propelled at the flow rate of 0.8 ml min(-1) The carrier was distilled water. The eluent contained 10(-2) M lithium acetate and 5×10(-3) M benzo-18-crown-6 (B18C6), and the reagent solution consisted of 5×10(-4) M 4-diethylamino-2, 5-dichloroazobenzene-4-sulfonate, 5×10(-3) M B18C6, 10(-3)M EDTA (dilithium salt) and 3×10(-3) M lithium hydroxide. The extraction solvent was a mixture of benzene and chlorobenzene (1+1). The absorbance was continuously measured at 450 nm with a 8 μl flow cell (path length: 10 mm). Calibration curves for sodium and potassium were linear in the range from 1×10(-4) M to 2×10(-3) M and from 5×10(-6) M to 1×10(-4) M, respectively. The sampling rate was about 20 samples per hour.
kn-abstract=シリカゲルカラムによるNa(+)とK(+)の分離及びクラウン錯体の陰イオン染料とのイオン会合体の溶媒抽出を組み込んだFIAによる吸光光度法を検討した.内経1mm,長さ20cmのPTFEチューブに100～200メッシュのシリカゲルを詰めたカラムを用いた.溶離液として,5×10(-3)Mベンゾ-18-クラウン-6及び,10(-2)M酢酸リチウムを含む水溶液を用いた.分析イオンを含む溶出液は試薬溶液(5×10(-4)Mの4'-ジエチルアミノ-2,5-ジクロロアゾベンゼン-4-スルホン酸イオン,5×10(-3)Mのベンゾ-18-クラウン-6,10(-3)MのEDTA及び3×10(-3)Mの水酸化リチウムを含む)と混合され,T字型セグメンターで抽出溶媒(ベンゼン+クロロベンゼン=1+1)と合流し,抽出コイル中で抽出が行われた.ポリテトラフルオロエチレン膜を備えた相分離器により,有機相は分離され,8μlのフローセルで450nmの吸光度が測定された.1×10(-4)M～2×10(-3)MのNa(+),5×10(-6)M～1×10(-4)MのK(+)に対して,検量線は直線となった.
en-copyright=
kn-copyright=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=1
ORCID=

en-aut-name=YonedaNaomi
en-aut-sei=Yoneda
en-aut-mei=Naomi
kn-aut-name=米田直生
kn-aut-sei=米田
kn-aut-mei=直生
aut-affil-num=2
ORCID=

en-aut-name=IwachidoTadashi
en-aut-sei=Iwachido
en-aut-mei=Tadashi
kn-aut-name=岩知道正
kn-aut-sei=岩知道
kn-aut-mei=正
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=solvent extraction/FIA
kn-keyword=solvent extraction/FIA
en-keyword=silica-gel column separation
kn-keyword=silica-gel column separation
en-keyword=sodium and potassium determination
kn-keyword=sodium and potassium determination
en-keyword=benzo-18 crown-6
kn-keyword=benzo-18 crown-6
en-keyword=4'-diethylaminophenyl-2
kn-keyword=4'-diethylaminophenyl-2
END

start-ver=1.4
cd-journal=joma
no-vol=38
cd-vols=
no-issue=3
article-no=
start-page=103
end-page=108
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=19890305
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Spectrophotometric determination of copper with iodide ion and Crystal Violet
kn-title=ヨウ化物イオンとクリスタルバイオレットを用いる銅の吸光光度定量
en-subtitle=
kn-subtitle=
en-abstract=A method for spectrophotometric determination of micro amounts of copper with iodide ion and Crystal Violet (CV) was developed. The method is based on the formation of triiodide ion by the oxidative reaction of iodide ion with copper(II) in dilute sulfuric acid solution and the extraction of triiodide ion into toluene as an ion association complex with CV. The main extracted species is expected to be an ion association complex, CV(+)I(-)(3) copper(II) acts as a catalyst. The recommended procedure is as follows. Take a sample solution containing copper(II) up to 1.6 μg into a 25-ml stoppered test tube. Add 1 ml of 0.46 M sodium iodide solution, 1 ml of 0.5 M sulfuric acid solution and 1 ml of 3.2×10(-4)M CV solution, and dilute the solution to 10 ml with distilled water. Shake the mixture with 5 ml of toluene for 8 min. After the phase separation, measure the absorbance of the organic phase at 607 nm against the reagent blank. The calibration graph shows linearity up to 2.5×10(-6) M of copper(II) in the aqueous phase, and the apparent molar absorptivity is 1.7×10(5)1 mol(-1) cm(-1). Small amounts of oxidizing and reducing agents such as Fe(III), Cr(III) and Co(II) interfered with the determination. The present method is very simple and has a high sensitivity.
kn-abstract=硫酸酸性溶液中でCu(2+)に対して過剰のI(-)が存在すると,I(-)は酸化されて三ヨウ化物イオン(I(-)(3))となり,Cu(2+)はジヨード銅(I)酸イオン(CuI(-)(2))となる.生成したI(-)(3),CuI(-)(2)はクリスタルバイオレット(CV(+))の存在下でイオン会合体となり,トルエンに抽出される.このトルエン相の607nmにおける吸光度を測定すれば,銅を定量することができる.検量線より算出した見掛けのモル吸光係数は1.7×10(5)1 mol(-1)cm(-1)であった.Fc(III),Cr(III),Co(II)などの酸化性及び還元性物質の共存は定量を妨害するが,その他多くのイオンは多量共存しても妨害とはならない.更に抽出化学種について検討した結果,主な抽出種はI(-)(3)とCV(+)の1:1のイオン会合体であり,大部分の銅イオンは水相に残っていることを確認した.これらのことから,本抽出系では銅(II)イオンは触媒的作用をしているものと結論された.
en-copyright=
kn-copyright=

en-aut-name=YamamotoKoichi
en-aut-sei=Yamamoto
en-aut-mei=Koichi
kn-aut-name=山本幸市
kn-aut-sei=山本
kn-aut-mei=幸市
aut-affil-num=1
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=米子工業高等専門学校工業化学科

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=spectrophotometric dtermination of copper with iodide ion and Crystal Violet
kn-keyword=spectrophotometric dtermination of copper with iodide ion and Crystal Violet
en-keyword=extraction of ion association complex into toluene
kn-keyword=extraction of ion association complex into toluene
END

start-ver=1.4
cd-journal=joma
no-vol=40
cd-vols=
no-issue=5
article-no=
start-page=T107
end-page=T112
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1991
dt-pub=19910505
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Spectrophotometric determination of sodium and potassium by FIA after separation on a cation exchanger column and solvent extraction
kn-title=陽イオン交換カラム分離/溶媒抽出法を用いるナトリウム及びカリウムイオンのフローインジェクション吸光光度法
en-subtitle=
kn-subtitle=
en-abstract=Sodium and potassium ions were spectrophotometrically determined by a solvent extraction/flow injection method incorporated with a cation exchanger column. The ion association complexes which formed between alkali metal-crown ether complexes and anionic dye were extracted into an organic phase, and the absorbance of the organic phase was measured after the phase separation by a phase separator with poly (tetrafluoroethylene) porous membrane. The manifold was composed of three streams: 1. a carrier stream, 2. an eluent stream, and 3. an extraction solvent stream. The eluent contained 4×10(-3)M EDTA·3Li. The extraction solvent (1+1 mixture of benzene and chlorobenzene) contained 3×10(-3)M tetrabromophenolphthalein ethyl ester and 2×10(-3)M dicyclohexano-18-crown-6. Sodium and potassium ions were separated on a cation exchanger column (2.5mm i.d.×12mm). The eluent was made alkaline (pH 10) by passing through the anion exchanger membrane tubing which was kept in a 0.1M LiOH solution. The absorbance of the organic phase was measured at 615nm. Calibration graphs were linear up to 5×10(-4)M for Na(+) and 8×10(-5)M for K(+), respectively. The sample throughput was 12h(-1). The procedure was applicable to river and tap water samples.
kn-abstract=Na(+), K(+)の溶媒抽出/吸光光度定量をFIAにより行った.両イオンの分離は低交換容量の陽イオン交換樹脂カラムにより行った.イオン交換樹脂は内径2.5mm,長さ12mmの樹脂製カラムに充てんして用いた.流れ系はキャリヤー,溶離液,抽出液の3流路を用い,試料はキャリヤーに注入した.キャリヤーは溶離液と合流し,カラムでNa(+)とK(+)は分離された.溶離液には4×10(-4)M EDTA・3Liを使用した.分析イオンを含む溶出液は,陰イオン交換膜チューブでアルカリ性となり,T字型セグメンターで有機相と合流し,抽出コイル内でNa(+), K(+)の抽出が行われた.有機相はイオン会合体形成用の染料陰イオンTBPE(テトラブロモフェノールフタレインエチルエステル)とクラウン化合物を含むベンゼン,クロロベンゼン(1+1)の混合溶媒である.ポリテトラフルオロエチレン膜を備えた相分離器により分離された有機相の吸光度は615nmで測定された. 5×10(-4)M以下のNa(+), 8×10(-5)M以下のK(+)に対して検量線は直線関係を示した.又本法による分析速度は毎時12試料であった.
en-copyright=
kn-copyright=

en-aut-name=YoshidaKo
en-aut-sei=Yoshida
en-aut-mei=Ko
kn-aut-name=吉田耕
kn-aut-sei=吉田
kn-aut-mei=耕
aut-affil-num=1
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=sodium and potassium determination
kn-keyword=sodium and potassium determination
en-keyword=spectrophotometry
kn-keyword=spectrophotometry
en-keyword=solvent extraction/flow injection analysis
kn-keyword=solvent extraction/flow injection analysis
en-keyword=cation exchanger column
kn-keyword=cation exchanger column
en-keyword=dicyclohexano-18-crown-6
kn-keyword=dicyclohexano-18-crown-6
en-keyword=tetrabromophenolphthalein ethyl ester
kn-keyword=tetrabromophenolphthalein ethyl ester
END

start-ver=1.4
cd-journal=joma
no-vol=45
cd-vols=
no-issue=7
article-no=
start-page=697
end-page=699
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19960705
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Direct photometric detection of inorganic anions by capillary zone electrophoresis using stacking effect of sulfate ion on sample ions
kn-title=硫酸イオンの試料イオンに対するスタッキング効果を利用する無機陰イオンのキャピラリー電気泳動/直接吸光分析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Stacking effect of sulfate ion on the analysis of inorganic anions by capillary zone electrophoresis was examined during the sample injection period. A used silica capillary was dynamically coated with tetradecyltrimethylammonium bromide (TDTMA(+)Br(-)) to control the electroosmotic flow. Analyte anions were directly detected by photometry at 214 nm. Five kinds of anions, namely bromide, nitrite, nitrate, molybdate, and tungstate, were detected. Anion separation was developed using 4×10(-3) M sodium sulfate in the carrier solution. Peak heights for anions increased along with additional Na(2)SO(4). The stacking effect was more effective for the anions with high mobility than those with low mobility. Calibration graphs for nitrate and nitrite showed good linearity in the concentration range of 10(-6) to 10(-5) M.
en-copyright=
kn-copyright=

en-aut-name=TakayanagiToshio
en-aut-sei=Takayanagi
en-aut-mei=Toshio
kn-aut-name=高柳俊夫
kn-aut-sei=高柳
kn-aut-mei=俊夫
aut-affil-num=1
ORCID=

en-aut-name=HiroiYasuko
en-aut-sei=Hiroi
en-aut-mei=Yasuko
kn-aut-name=廣井康子
kn-aut-sei=廣井
kn-aut-mei=康子
aut-affil-num=2
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=capillary zone electrophoresis
kn-keyword=capillary zone electrophoresis
en-keyword=inorganic anion
kn-keyword=inorganic anion
en-keyword=stacking effect
kn-keyword=stacking effect
en-keyword=direct photometric detection
kn-keyword=direct photometric detection
END

start-ver=1.4
cd-journal=joma
no-vol=48
cd-vols=
no-issue=2
article-no=
start-page=253
end-page=259
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1999
dt-pub=19990205
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Development of a new gas-permeation system and its application to the spectrophotometric determination of ammonium ion by FIA
kn-title=精密分析用ガス透過システムの設計とガス透過/フローインジェクション法によるアンモニア態窒素の吸光光度定量
en-subtitle=
kn-subtitle=
en-abstract=ガス透過分離におけるガス透過の長期間の安定性と高効率化,更にガス透過効率の長期間の再現性を向上させた,新しいオンラインガス透過システムを構築した.このシステムでは新しく設計・製作したガス透過ユニットを組み込み,ユニット中を溶液が下方から上方に流れるようにし,気泡の発生を防ぐ工夫がほどこされ,更に測定後にガス透過ユニット内に滞留する溶液を排除するために2個の六方切り替えバルブを内蔵させている.ガスの安定的透過のために温度制御できる小型恒温槽を装着した.この装置中にガス透過ユニット,アンモニウムイオン定量用の反応コイルなどを組み入れ,精密測定の向上を目指した.ガス透過ユニットは,多孔質ポリテトラフルオロエチレン(PTFE)チューブとガラス管からなり,接続には樹脂製フェラル及びO-リングを用い,組み立てを容易にし,デッドボリュームも小さくした.ガス透過ユニット,反応コイル及び試料注入器などを恒温槽の中で一定温度に保つことにより,安定した再現性の良い測定が可能となった.ガス透過ユニット内に滞留する溶液を簡単かつ迅速に強制排除する機能も備えており,測定後はガス透過ユニット中の溶液を除いておくことにより,多孔質チューブの透過性能が長期間維持され,再活性化の操作をすることなく,高いガス透過効率を維持することができた.本システムを用いると,検量線は0～10,0～1.0ppmの範囲で良好な直線性を示し,1時間当たり30試料の分析が可能となった.実際に,河川水中のアンモニウムイオンの定量を行ったところ,インドフェノール誘導体/FIA/吸光光度法による定量値と良く一致し,試料に対する相対標準偏差は0.51,0.83%で,回収率は97～98%と良好であった.
kn-abstract=A new on-line gas-permeation system was developed to enhance the efficiency and stability of gas permeation for a long time, and was applied to the determination of ammonium ion in river-water samples by the flow- injection method. The proposed gas-permeation system consists of a newly designed gas-permeation unit, a sample injection valve, a reaction coil and two six-way rotary valves in a constant-temperature oven. The gas- permeation unit was assembled using an inner micro porous polytetrafluoroethylene (PTFE) membrane tubing and an outer glass tube, which could be easily connected with one another using connectors with ferrules and o-rings. A sample solution was injected into a carrier containing 0.02 M sodium hydroxide, in which ammonium ion was converted to gaseous ammonia. The gaseous ammonia generated in the carrier passed through the micro porous PTFE membrane, was absorbed in a reagent solution and changed the pH of the reagent solution, which resulted in a color change to cresol red. The absorbance change of cresol red at 550 nm was measured using a visible detector with a flow cell (8μl, 10mm path). After the measurement, two six-way rotary valves were switched over from the flowing state to the drain state of the gas-permeation unit, and the residual solutions in the gas-permeation unit were removed and the PTFE membrane tubing was dried. By keeping the temperature of the oven at 40℃, the sensitivity and the reproducibility for the determination of ammonium ion was improved. Furthermore, the efficiency of gas permeation through the inner tubing could be kept high and constant for a long period without any procedure for reactivating the porous PTFE membrane tubing. Calibration graphs for ammonium ion were liner over ranges of 0 to 10 ppm and 0 to 1.0 ppm of N-NH(4)(+), and the sampling rate was 30 samples per hour. The detection limit for N-NH(4)(+) was about 0.01 ppm when the effective length of the membrane tubing was 5 cm. By using the proposed FIA system, ammonium ion in river-water samples was determined. The analytical results obtained by the proposed method showed a good correlation with those obtained using the indophenol derivatization/FIA method. The relative standard deviations of ten injections were 0.51% and 0.83% with concentrations of 2.73 ppm and 4.11 ppm, respectively. The recoveries of ammonium ion were found to be 97～98%.
en-copyright=
kn-copyright=

en-aut-name=HiguchiKeiro
en-aut-sei=Higuchi
en-aut-mei=Keiro
kn-aut-name=樋口慶郎
kn-aut-sei=樋口
kn-aut-mei=慶郎
aut-affil-num=1
ORCID=

en-aut-name=InoueAkiko
en-aut-sei=Inoue
en-aut-mei=Akiko
kn-aut-name=井上亜希子
kn-aut-sei=井上
kn-aut-mei=亜希子
aut-affil-num=2
ORCID=

en-aut-name=TsuboiTomonori
en-aut-sei=Tsuboi
en-aut-mei=Tomonori
kn-aut-name=坪井知則
kn-aut-sei=坪井
kn-aut-mei=知則
aut-affil-num=3
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=東京化成工業(株)FIA研究グループ

affil-num=3
en-affil=
kn-affil=中国電力(株)技術研究センター

affil-num=4
en-affil=
kn-affil=岡山大学
en-keyword=gas permeation system
kn-keyword=gas permeation system
en-keyword=microporous polytetrafluoroethylene membrane tubing
kn-keyword=microporous polytetrafluoroethylene membrane tubing
en-keyword=FIA with gas permeation method
kn-keyword=FIA with gas permeation method
en-keyword=determination of ammonium ion
kn-keyword=determination of ammonium ion
en-keyword=river water sample
kn-keyword=river water sample
END

start-ver=1.4
cd-journal=joma
no-vol=51
cd-vols=
no-issue=9
article-no=
start-page=791
end-page=795
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=20020905
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Determination of trace metal impurities in nickel and iron salts using 2-(5-Nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropyl-amino)phenol by capillary zone electrophoresis
kn-title=2-(5-ニトロ-2-ピリジルアゾ)-5-(N-プロピル-N-スルホプロピルアミノ)フェノールを用いるキャピラリー電気泳動法によるニッケル, 鉄塩中の極微量不純物の定量
en-subtitle=
kn-subtitle=
en-abstract=市販金属塩中の極微量金属不純物の定量法として, 2-(5-ニトロ-2-ピリジルアゾ)-5-(N-プロピル-N-スルホプロピルアミノ)フェノール (Nitro-PAPS) とのキレートを分離, 検出するキャピラリー電気泳動法について検討した. 各種金属イオンと定量的に反応するpH7の条件では, 高感度検出が可能であるが, 多くの金属錯体の分離は十分でない. そこで, 電気浸透流やキレートの電気泳動移動度を変化させる効果のある第四級アンモニウム塩等の添加を検討し, 種々な相互作用試薬を用いて分離の改善を図った. その結果, 臭化セチルトリメチルアンモニウムの添加で電気浸透流が抑制され, 硫酸ナトリウムの添加によるスタッキング効果の活用によりシグナルが鋭敏化され, 尿素の添加でニトロ基周りの水和を抑制したところ, 金属キレートの分離性能が向上した. 本法により10(-7)MレベルのFe, Co, Ni, Cuの分離定量が可能になり, 市販特級金属塩中の極微量金属の分析に適用したところ, ppm (=10(-6)g/g) レベルの金属不純物を定量することができた.
kn-abstract=2-(5-Nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol (Nitro-PAPS) was used for a quantitative determination of ultratrace-level metal ions in commercially available metal salts by capillary zone electrophoresis. At pH 7, Nitro-PAPS reacts with various metal ions to form chelate compounds having large molar absorptivities. Although chelate formation is useful for the highly sensitive detection of metal ions, the resolution of the metal complexes is poor at pH 7. Cetyltrimethylammonium bromide was added to suppress any electroosmotic flow. The hydration of nitro groups of a chelating agent and chelates was controlled by the addition of urea. Sodium sulfate was also utilized to sharpen the signals of metal chelates by a field-enhanced stacking effect. As a result, the resolution was improved and the determination of 10-7 M level metal ions was possible. The proposed method was applied to the analysis of metal impurity at ultratrace levels in commercially available nickel and iron salts.
en-copyright=
kn-copyright=

en-aut-name=KagawaTakuji
en-aut-sei=Kagawa
en-aut-mei=Takuji
kn-aut-name=香川拓司
kn-aut-sei=香川
kn-aut-mei=拓司
aut-affil-num=1
ORCID=

en-aut-name=TakayanagiToshio
en-aut-sei=Takayanagi
en-aut-mei=Toshio
kn-aut-name=高柳俊夫
kn-aut-sei=高柳
kn-aut-mei=俊夫
aut-affil-num=2
ORCID=

en-aut-name=OshimaMitsuko
en-aut-sei=Oshima
en-aut-mei=Mitsuko
kn-aut-name=大島光子
kn-aut-sei=大島
kn-aut-mei=光子
aut-affil-num=3
ORCID=

en-aut-name=MotomizuShoji
en-aut-sei=Motomizu
en-aut-mei=Shoji
kn-aut-name=本水昌二
kn-aut-sei=本水
kn-aut-mei=昌二
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学
en-keyword=capillary zone electrophoresis
kn-keyword=capillary zone electrophoresis
en-keyword=metal ions
kn-keyword=metal ions
en-keyword=2-(5-nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol
kn-keyword=2-(5-nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol
en-keyword=nickel and iron salt
kn-keyword=nickel and iron salt
en-keyword=impurity analysis
kn-keyword=impurity analysis
END