| ID | 17429 |
| Eprint ID | 17429
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| FullText URL | |
| Title Alternative | Purification and properties of a high molecular weight acid soluble nuclear protein from mouse ascites sarcoma cells
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| Author | |
| Abstract | A high molecular weight acid soluble nuclear protein (HAN-1) was isolated in a electrophoretically homogeneous state from mouse ascites sarcoma cells (SR-C3H cells) by the exclusion chromatography in Sephacryl S-200 and the DEAE-Sephadex chromatography of the nuclear 0.2M H(2)SO(4) extract. The content of HAN-1 in SR-C3H nuclei was about 4.3% per total histone, the highest among the cell types tested. The molecular weight of HAN-1 was estimated to be 125,000 by SDS-polyacrylamide gel electrophoresis. The amino acid composition of HAN-1 was rich in glutamic acid, alanine, lysine and aspartic acid, the acidic/basic amino acid ratio 1.8. The in vitro RNA synthesizing activity of SR-C3H cell RNA polymerases Ⅰ and Ⅱ on a naked DNA template was differentially affected by HAN-1; the reaction with polymerase I was inhibited and that with polymerase Ⅱ stimulated.
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| Published Date | 1978-08
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| Publication Title |
岡山医学会雑誌
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| Publication Title Alternative | Journal of Okayama Medical Association
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| Volume | volume90
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| Issue | issue7-8
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| Publisher | 岡山医学会
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| Publisher Alternative | Okayama Medical Association
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| Start Page | 999
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| End Page | 1013
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| ISSN | 0030-1558
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| NCID | AN00032489
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| Content Type |
Journal Article
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| Official Url | https://www.jstage.jst.go.jp/article/joma1947/90/7-8/90_7-8_999/_article/-char/ja/
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| Related Url | http://www.okayama-u.ac.jp/user/oma/
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| language |
Japanese
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| Copyright Holders | 岡山医学会
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| File Version | publisher
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| Refereed |
True
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| Eprints Journal Name | joma
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