| ID | 16340 |
| Eprint ID | 16340
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| FullText URL | |
| Title Alternative | Measurement of circulating immune complexes using Fc receptors of guinea pig peritoneal exudate cells. Part 1. Establishment of the method for measuring of immune complexes.
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| Author |
Saito, Yoshihito
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| Abstract | A new method for detecting circulating IgG immune complexes (IC) using guinea pig peritoneal exudate cells (PEC) has been established. Binding of (125)I BSA-anti BSA IgG IC to PEC was inhibited by serum containing IC in a dose dependent fashion. A 100μl aliquot of PEC suspension (2.5X10(6) cells/ml) was preincubated with diluted five fold serum for 60 minutes at 4℃. After washing pretreated PEC 3 times, 100μl of (125)I labelled 3 times antigen excess-IC was added and incubated for 90 minutes at 4℃. At the end of incubation, the suspension was centrifuged at 10,000G for 1 minute, and the radioactivity of the pellet was measured. This method mainly utilizes the reaction between Fc receptors on PEC and the Fc portion of the complexed IgG.
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| Keywords | immune complex
guinea pig peritoneal exudate cell
Fc receptor
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| Published Date | 1983-08-31
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| Publication Title |
岡山医学会雑誌
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| Publication Title Alternative | Journal of Okayama Medical Association
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| Volume | volume95
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| Issue | issue7-8
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| Publisher | 岡山医学会
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| Publisher Alternative | Okayama Medical Association
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| Start Page | 653
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| End Page | 663
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| ISSN | 0030-1558
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| NCID | AN00032489
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| Content Type |
Journal Article
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| Official Url | https://www.jstage.jst.go.jp/article/joma1947/95/7-8/95_7-8_653/_article/-char/ja/
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| Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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| language |
Japanese
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| Copyright Holders | 岡山医学会
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| File Version | publisher
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| Refereed |
True
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| Eprints Journal Name | joma
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