start-ver=1.4
cd-journal=joma
no-vol=145
cd-vols=
no-issue=
article-no=
start-page=105021
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2026
dt-pub=202603
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Assessing the role of folate syntrophy and folate cross-feeding in the pathobiology of infectious-inflamed milieu caused by Fusobacterium nucleatum
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Diet and nutrition affect almost every biological process, including multiple chronic diseases, diabetes, and some cancers. However, there are still significant gaps in our understanding of the importance of nutrition and healthy diets in syntrophy with respect to cross-feeding of the microbe-microbe and the microbe-host in the pathobiology of the infectious-inflamed intestinal milieu caused by anaerobic opportunistic bacteria such as Fusobacterium nucleatum (F. nucleatum). We examined the immune outcomes of three-member folate syntrophy and cross-feeding between F. nucleatum bacteria, endogenous folate-producing gut bacteria, and host cells at the host-pathogen interface using a triple co-culture model. T84, THP-1, and Huh7 cells were inoculated with F. nucleatum for 6 h in regular DMEM, DMEM with 9.5 μM folic acid, or with/without a mixture of Bifidobacterium longum subsp. infantis (B. infantis) and Escherichia coli Nissle 1917 (EcN). Cytokine secretion, cometabolite levels (ammonia, indoles), cell viability, and barrier integrity were assessed. F. nucleatum-induced folate depletion was associated with increased IL-1β and IL-6 and decreased IL-22, along with reduced transepithelial electrical resistance (TEER) and cell viability in T84 cells. Folate supplementation mitigated these effects. The mixture of B. infantis and EcN reduced F. nucleatum-induced pro-inflammatory cytokines, increased IL-22, and improved TEER and cell viability. These protective effects were enhanced by the addition of folate. F. nucleatum also elevated ammonia and reduced indoles, effects reversed by B. infantis and EcN. In addition to the intrinsic pathogenicity of harmful bacteria, folate deprivation, microbe?microbe folate syntrophy, and microbe?host folate cross-feeding contribute to the pathobiology of anaerobic opportunistic bacteria and influence the physiological fate of host cells. A combination of B. infantis and EcN modulates the infectious-inflamed interface through a cytoprotective effect and mechanical competitive extrusion of pathogenic F. nucleatum. These results provide potential insights into the mechanisms of early-onset colorectal cancer, and evidently, require future studies using patient-derived organoids and in vivo systems to improve clinical relevance.
en-copyright=
kn-copyright=

en-aut-name=GhadimiDarab
en-aut-sei=Ghadimi
en-aut-mei=Darab
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Bl?merSophia
en-aut-sei=Bl?mer
en-aut-mei=Sophia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=?ahin KayaAysel
en-aut-sei=?ahin Kaya
en-aut-mei=Aysel
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Kr?gerSandra
en-aut-sei=Kr?ger
en-aut-mei=Sandra
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=R?ckenChristoph
en-aut-sei=R?cken
en-aut-mei=Christoph
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=Sch?ferHeiner
en-aut-sei=Sch?fer
en-aut-mei=Heiner
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=MatsuzakiShigenobu
en-aut-sei=Matsuzaki
en-aut-mei=Shigenobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=BockelmannWilhelm
en-aut-sei=Bockelmann
en-aut-mei=Wilhelm
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Microbiology and Biotechnology, Max Rubner-Institut
kn-affil=

affil-num=2
en-affil=Faculty of Medicine, Christian-Albrechts-University of Kiel
kn-affil=

affil-num=3
en-affil=Department of Nutrition and Dietetics, Faculty of Health Sciences, Antalya Bilim University
kn-affil=

affil-num=4
en-affil=Institute of Pathology, Kiel University, University Hospital, Schleswig-Holstein
kn-affil=

affil-num=5
en-affil=Institute of Pathology, Kiel University, University Hospital, Schleswig-Holstein
kn-affil=

affil-num=6
en-affil=Laboratory of Molecular Gastroenterology & Hepatology, Christian-Albrechts-University & UKSH Campus Kiel
kn-affil=

affil-num=7
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Department of Medical Laboratory Science, Faculty of Health Sciences, Kochi Gakuen University
kn-affil=

affil-num=9
en-affil=Department of Microbiology and Biotechnology, Max Rubner-Institut
kn-affil=
en-keyword=Nutrition
kn-keyword=Nutrition
en-keyword=Metaflammation
kn-keyword=Metaflammation
en-keyword=Folate
kn-keyword=Folate
en-keyword=Cytokines
kn-keyword=Cytokines
en-keyword=Infection
kn-keyword=Infection
en-keyword=Host cells
kn-keyword=Host cells
END

start-ver=1.4
cd-journal=joma
no-vol=142
cd-vols=
no-issue=
article-no=
start-page=104967
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202506
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cross-feeding between beneficial and pathogenic bacteria to utilize eukaryotic host cell-derived sialic acids and bacteriophages shape the pathogen-host interface milieu
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Under an inflamed-intestinal milieu, increased free sialic acids are associated with the overgrowth of some pathogenic bacterial strains. Recently, the protective immunomodulatory activity of gut bacteriophages (phages) has also been highlighted. However, the role of phages in triple reciprocal interactions between pathogenic bacteria, beneficial bacteria, and their host cell sialic acids has not been studied so far. We established a sialidase-explicit model in which beneficial and pathogenic bacteria interact through cross-feeding and competition for free sialic acid using a human triple co-culture cell model incorporating colonocytes (T84 cells), monocytes (THP-1 cells), and hepatocytes (Huh7 cells). Triple co-cultured cells were challenged with Gram-positive Bifidobacterium bifidum (B. bifidum) and Gram-negative Pseudomonas aeruginosa PAO1 (P. a PAO1) in the absence or presence of its KPP22 phage in two different cell culture mediums: 1) standard Dulbecco's Modified Eagle Medium (DMEM) and 2) DMEM with 2,3-dehydro-2-deoxy-N-acetylneuraminic acid (DANA). Changes in physiological, functional, and structural health markers of stimulated cocultured cells were evaluated. The concentrations of sialic acid and pro-inflammatory cytokines in the cell culture supernatants were quantified. P. a PAO1 triggered the release of interleukin 6 and 8 (IL-6 and IL-8), accompanied by increased levels of free sialic acid, reduced viability of co-cultured cells, and disrupted the integrity of the cellular monolayer. These disruptive effects were markedly attenuated by KPP22 phage and B. bifidum. In addition to well-documented differences in the structure and composition of the bacterial cell walls of Gram-negative pathogenic bacteria and bifidobacteria, two distinct factors seem to be pivotal in modulating the pathogen-host interface milieu: (i) the presence of phages and (ii) the utilization of free sialic acids secreted from host cells by bifidobacteria.
en-copyright=
kn-copyright=

en-aut-name=GhadimiDarab
en-aut-sei=Ghadimi
en-aut-mei=Darab
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=F?lster-HolstRegina
en-aut-sei=F?lster-Holst
en-aut-mei=Regina
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Bl?merSophia
en-aut-sei=Bl?mer
en-aut-mei=Sophia
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=EbsenMichael
en-aut-sei=Ebsen
en-aut-mei=Michael
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=R?ckenChristoph
en-aut-sei=R?cken
en-aut-mei=Christoph
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MatsuzakiShigenobu
en-aut-sei=Matsuzaki
en-aut-mei=Shigenobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=BockelmannWilhelm
en-aut-sei=Bockelmann
en-aut-mei=Wilhelm
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=Department of Microbiology and Biotechnology, Max Rubner-Institut
kn-affil=

affil-num=2
en-affil=Clinic of Dermatology, Venerology und Allergology, University Hospital Schleswig-Holstein
kn-affil=

affil-num=3
en-affil=Clinic of Dermatology, Venerology und Allergology, University Hospital Schleswig-Holstein
kn-affil=

affil-num=4
en-affil=St?dtisches MVZ Kiel GmbH (Kiel City Hospital), Department of Pathology
kn-affil=

affil-num=5
en-affil=Institute of Pathology, Kiel University, University Hospital, Schleswig-Holstein
kn-affil=

affil-num=6
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Department of Medical Laboratory Science, Faculty of Health Sciences, Kochi Gakuen University
kn-affil=

affil-num=8
en-affil=Department of Microbiology and Biotechnology, Max Rubner-Institut
kn-affil=
en-keyword=Bacterial sialidase
kn-keyword=Bacterial sialidase
en-keyword=Inflammation
kn-keyword=Inflammation
en-keyword=Cytokines
kn-keyword=Cytokines
en-keyword=Infection
kn-keyword=Infection
en-keyword=Bifidobacteria
kn-keyword=Bifidobacteria
en-keyword=Phages
kn-keyword=Phages
END

start-ver=1.4
cd-journal=joma
no-vol=79
cd-vols=
no-issue=4
article-no=
start-page=231
end-page=242
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202508
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Bloodstream Infections Caused by Gram-Negative Bacteria in Geriatric Patients: Epidemiology, Antimicrobial Resistance and The Factors Affecting Mortality
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bloodstream infections (BSIs) are an important cause of morbidity and mortality in geriatric patients. We retrospectively analyzed the cases of geriatric patients who developed BSIs due to gram-negative bacteria in order to evaluate the epidemiology, antimicrobial resistance, and the factors affecting mortality. The cases of 110 patients aged ? 65 years admitted to our hospital between January 1, 2017, and December 31, 2022 were assessed; 70 (63.6%) of the BSIs were healthcare-associated BSIs. The urinary system was the most common detectable source of infection at 43.6%. The most frequently isolated bacteria were Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae, in that order. Carbapenem resistance was detected in 17 patients (15.5%), and extended-spectrum beta-lactamase (ESBL) production from Enterobacterales family members was detected in 37 (51.4%) patients. Multivariate analysis revealed that (i) the probability of mortality in the patients with total bilirubin was increased by approx. sixfold and (ii) the likelihood of mortality for those with a Pitt bacteremia score (PBS) ? 4 points was approx. 17 times higher. PBS and simplified qPitt scores can help predict mortality and manage geriatric patients. There is a significant increase in mortality among patients with procalcitonin (PCT) levels at ? 2 nm/ml.
en-copyright=
kn-copyright=

en-aut-name=KardanM Enes 
en-aut-sei=Kardan
en-aut-mei=M Enes 
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ErdemIlknur
en-aut-sei=Erdem
en-aut-mei=Ilknur
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YildizEmre
en-aut-sei=Yildiz
en-aut-mei=Emre
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KirazNuri
en-aut-sei=Kiraz
en-aut-mei=Nuri
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=?elikkolAliye
en-aut-sei=?elikkol
en-aut-mei=Aliye
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Department of Infectious Diseases, Faculty of Medicine, Namik Kemal University
kn-affil=

affil-num=2
en-affil=Department of Infectious Diseases, Faculty of Medicine, Namik Kemal University
kn-affil=

affil-num=3
en-affil=Department of Infectious Diseases, Faculty of Medicine, Namik Kemal University
kn-affil=

affil-num=4
en-affil=Department of Medical Microbiology, Faculty of Medicine, Namik Kemal University
kn-affil=

affil-num=5
en-affil=Department of Biochemistry, Faculty of Medicine, Namik Kemal University
kn-affil=
en-keyword=geriatrics
kn-keyword=geriatrics
en-keyword=gram-negative bacteria
kn-keyword=gram-negative bacteria
en-keyword=epidemiology
kn-keyword=epidemiology
en-keyword=antimicrobial resistance
kn-keyword=antimicrobial resistance
en-keyword=mortality
kn-keyword=mortality
END

start-ver=1.4
cd-journal=joma
no-vol=65
cd-vols=
no-issue=11
article-no=
start-page=1769
end-page=1786
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240824
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Nutrient Requirements Shape the Preferential Habitat of Allorhizobium vitis VAR03-1, a Commensal Bacterium, in the Rhizosphere of Arabidopsis thaliana
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A diverse range of commensal bacteria inhabit the rhizosphere, influencing host plant growth and responses to biotic and abiotic stresses. While root-released nutrients can define soil microbial habitats, the bacterial factors involved in plant?microbe interactions are not well characterized. In this study, we investigated the colonization patterns of two plant disease biocontrol agents, Allorhizobium vitis VAR03-1 and Pseudomonas protegens Cab57, in the rhizosphere of Arabidopsis thaliana using Murashige and Skoog (MS) agar medium. VAR03-1 formed colonies even at a distance from the roots, preferentially in the upper part, while Cab57 colonized only the root surface. The addition of sucrose to the agar medium resulted in excessive proliferation of VAR03-1, similar to its pattern without sucrose, whereas Cab57 formed colonies only near the root surface. Overgrowth of both bacterial strains upon nutrient supplementation inhibited host growth, independent of plant immune responses. This inhibition was reduced in the VAR03-1 ΔrecA mutant, which exhibited increased biofilm formation, suggesting that some activities associated with the free-living lifestyle rather than the sessile lifestyle may be detrimental to host growth. VAR03-1 grew in liquid MS medium with sucrose alone, while Cab57 required both sucrose and organic acids. Supplementation of sugars and organic acids allowed both bacterial strains to grow near and away from Arabidopsis roots in MS agar. These results suggest that nutrient requirements for bacterial growth may determine their growth habitats in the rhizosphere, with nutrients released in root exudates potentially acting as a limiting factor in harnessing microbiota.
en-copyright=
kn-copyright=

en-aut-name=HemeldaNiarsi Merry
en-aut-sei=Hemelda
en-aut-mei=Niarsi Merry
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=BaoJiyuan
en-aut-sei=Bao
en-aut-mei=Jiyuan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WatanabeMegumi
en-aut-sei=Watanabe
en-aut-mei=Megumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MatsuiHidenori
en-aut-sei=Matsui
en-aut-mei=Hidenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=IchinoseYuki
en-aut-sei=Ichinose
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=NoutoshiYoshiteru
en-aut-sei=Noutoshi
en-aut-mei=Yoshiteru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=7
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Commensal bacteria
kn-keyword=Commensal bacteria
en-keyword=Nutrient requirements
kn-keyword=Nutrient requirements
en-keyword=Organic acids
kn-keyword=Organic acids
en-keyword=Plant-microbe interactions
kn-keyword=Plant-microbe interactions
en-keyword=Rhizosphere
kn-keyword=Rhizosphere
en-keyword=Sugars
kn-keyword=Sugars
END

start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=
article-no=
start-page=921635
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220707
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Siderophore for Lanthanide and Iron Uptake for Methylotrophy and Plant Growth Promotion in Methylobacterium aquaticum Strain 22A
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Methylobacterium and Methylorubrum species are facultative methylotrophic bacteria that are abundant in the plant phyllosphere. They have two methanol dehydrogenases, MxaF and XoxF, which are dependent on either calcium or lanthanides (Lns), respectively. Lns exist as insoluble minerals in nature, and their solubilization and uptake require a siderophore-like substance (lanthanophore). Methylobacterium species have also been identified as plant growth-promoting bacteria although the actual mechanism has not been well-investigated. This study aimed to reveal the roles of siderophore in Methylobacterium aquaticum strain 22A in Ln uptake, bacterial physiology, and plant growth promotion. The strain 22A genome contains an eight-gene cluster encoding the staphyloferrin B-like (sbn) siderophore. We demonstrate that the sbn siderophore gene cluster is necessary for growth under low iron conditions and was complemented by supplementation with citrate or spent medium of the wild type or other strains of the genera. The siderophore exhibited adaptive features, including tolerance to oxidative and nitrosative stress, biofilm formation, and heavy metal sequestration. The contribution of the siderophore to plant growth was shown by the repressive growth of duckweed treated with siderophore mutant under iron-limited conditions; however, the siderophore was dispensable for strain 22A to colonize the phyllosphere. Importantly, the siderophore mutant could not grow on methanol, but the siderophore could solubilize insoluble Ln oxide, suggesting its critical role in methylotrophy. We also identified TonB-dependent receptors (TBDRs) for the siderophore-iron complex, iron citrate, and Ln, among 12 TBDRs in strain 22A. Analysis of the siderophore synthesis gene clusters and TBDR genes in Methylobacterium genomes revealed the existence of diverse types of siderophores and TBDRs. Methylorubrum species have an exclusive TBDR for Ln uptake that has been identified as LutH. Collectively, the results of this study provide insight into the importance of the sbn siderophore in Ln chelation, bacterial physiology, and the diversity of siderophore and TBDRs in Methylobacterium species.
en-copyright=
kn-copyright=

en-aut-name=JumaPatrick Otieno
en-aut-sei=Juma
en-aut-mei=Patrick Otieno
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=FujitaniYoshiko
en-aut-sei=Fujitani
en-aut-mei=Yoshiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AlessaOla
en-aut-sei=Alessa
en-aut-mei=Ola
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OyamaTokitaka
en-aut-sei=Oyama
en-aut-mei=Tokitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YurimotoHiroya
en-aut-sei=Yurimoto
en-aut-mei=Hiroya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SakaiYasuyoshi
en-aut-sei=Sakai
en-aut-mei=Yasuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TaniAkio
en-aut-sei=Tani
en-aut-mei=Akio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=3
en-affil= Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Science, Kyoto University
kn-affil=

affil-num=5
en-affil=Graduate School of Agriculture, Kyoto University
kn-affil=

affil-num=6
en-affil=Graduate School of Agriculture, Kyoto University
kn-affil=

affil-num=7
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
en-keyword=Methylobacterium species
kn-keyword=Methylobacterium species
en-keyword=lanthanide
kn-keyword=lanthanide
en-keyword=lanthanophore
kn-keyword=lanthanophore
en-keyword=siderophore
kn-keyword=siderophore
en-keyword=plant growth promoter
kn-keyword=plant growth promoter
en-keyword=heavy metal sequestration
kn-keyword=heavy metal sequestration
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=5
article-no=
start-page=549
end-page=556
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202110
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Glial Cells as Possible Targets of Neuroprotection through Neurotrophic and Antioxidative Molecules in the Central and Enteric Nervous Systems in Parkinson’s Disease
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide. The loss of nigrostriatal dopaminergic neurons produces its characteristic motor symptoms, but PD patients also have non-motor symptoms such as constipation and orthostatic hypotension. The pathological hallmark of PD is the presence of α-synuclein-containing Lewy bodies and neurites in the brain. However, the PD pathology is observed in not only the central nervous system (CNS) but also in parts of the peripheral nervous system such as the enteric nervous system (ENS). Since constipation is a typical prodromal non-motor symptom in PD, often preceding motor symptoms by 10-20 years, it has been hypothesized that PD pathology propagates from the ENS to the CNS via the vagal nerve. Discovery of pharmacological and other methods to halt this progression of neurodegeneration in PD has the potential to improve millions of lives. Astrocytes protect neurons in the CNS by secretion of neurotrophic and antioxidative factors. Similarly, astrocyte-like enteric glial cells (EGCs) are known to secrete neuroprotective factors in the ENS. In this article, we summarize the neuroprotective function of astrocytes and EGCs and discuss therapeutic strategies for the prevention of neurodegeneration in PD targeting neurotrophic and antioxidative molecules in glial cells.
en-copyright=
kn-copyright=

en-aut-name=IsookaNami
en-aut-sei=Isooka
en-aut-mei=Nami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiyazakiIkuko
en-aut-sei=Miyazaki
en-aut-mei=Ikuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AsanumaMasato
en-aut-sei=Asanuma
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Parkinson’s disease
kn-keyword=Parkinson’s disease
en-keyword=astrocyte
kn-keyword=astrocyte
en-keyword=enteric glial cell
kn-keyword=enteric glial cell
en-keyword=neurotrophic factor
kn-keyword=neurotrophic factor
en-keyword=antioxidative molecule
kn-keyword=antioxidative molecule
END

start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=2
article-no=
start-page=153
end-page=167
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202104
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Lactoferrin-like Immunoreactivity in Distinct Neuronal Populations in the Mouse Central Nervous System
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Lactoferrin (Lf) is an iron-binding glycoprotein mainly found in exocrine secretions and the secondary granules of neutrophils. In the central nervous system (CNS), expression of the Lf protein has been reported in the lesions of some neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis, as well as in the aged brain. Lf is primarily considered an iron chelator, protecting cells from potentially toxic iron or iron-requiring microorganisms. Other biological functions of Lf include immunomodulation and transcriptional regulation. However, the roles of Lf in the CNS have yet to be fully clarified. In this study, we raised an antiserum against mouse Lf and investigated the immunohistochemical localization of Lf-like immunoreactivity (Lf-LI) throughout the CNS of adult mice. Lf-LI was found in some neuronal populations throughout the CNS. Intense labeling was found in neurons in the olfactory systems, hypothalamic nuclei, entorhinal cortex, and a variety of brainstem nuclei. This study provides detailed information on the Lf-LI distribution in the CNS, and the findings should promote further understanding of both the physiological and pathological significance of Lf in the CNS.
en-copyright=
kn-copyright=

en-aut-name=ShimaokaShigeyoshi
en-aut-sei=Shimaoka
en-aut-mei=Shigeyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HamaokaHitomi
en-aut-sei=Hamaoka
en-aut-mei=Hitomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=InoueJunji
en-aut-sei=Inoue
en-aut-mei=Junji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=AsanumaMasato
en-aut-sei=Asanuma
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TooyamaIkuo
en-aut-sei=Tooyama
en-aut-mei=Ikuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KondoYoichi
en-aut-sei=Kondo
en-aut-mei=Yoichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=2
en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=3
en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University
kn-affil=

affil-num=4
en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=5
en-affil=Molecular Neuroscience Research Center, Shiga University of Medical Science
kn-affil=

affil-num=6
en-affil=Molecular Neuroscience Research Center, Shiga University of Medical Science
kn-affil=
en-keyword=lactoferrin
kn-keyword=lactoferrin
en-keyword=immunohistochemistry
kn-keyword=immunohistochemistry
en-keyword=brain mapping
kn-keyword=brain mapping
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=1
article-no=
start-page=3
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210106
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Immunohistochemical study for the expression of leukocyte adhesion molecules, and FGF23 and ACE2 in P. gingivalis LPS-induced diabetic nephropathy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Objective</br>
The present study aims to examine the expression of leukocyte adhesion molecules and renal metabolic factors in diabetic mouse kidneys with periodontal pathogen Pg-LPS-induced nephropathy.</br>
Background</br>
We recently reported that the glomerular endothelium expresses toll-like receptor (TLR)2 and TLR4 in diabetic environments and TLR2/4 ligand Porphyromonas (P.) gingivalis lipopolysaccharides (Pg-LPS) induce nephropathy in diabetic mice. It is thought that Pg-LPS promotes the chronic inflammation with the overexpression of leukocyte adhesion molecules and renal-specific metabolic enzymes by the recognition of Pg-LPS via TLR in the diabetic kidneys. There have been no reports of the effects of periodontopathic bacteria on the expression of leukocyte adhesion molecules and the accumulation of physiologically active substances in the kidney.</br>
Methods</br>
The immunohistochemical investigation was performed on diabetic mouse kidney with Pg-LPS-induced nephropathy with glomerulosclerosis in glomeruli.</br>
Results</br>
There were no vessels which expressed vascular cell adhesion molecule-1 (VCAM-1), E-selectin, or fibroblast growth factor (FGF) 23 in streptozotocin (STZ)-induced diabetic ICR mice (STZ-ICR), or in healthy ICR mice administered Pg-LPS (LPS-ICR). However, in diabetic ICR mouse kidneys with Pg-LPS-induced nephropathy (LPS-STZ) the expression of VCAM-1 and the accumulation of FGF23 were observed in renal tubules and glomeruli, and the expression of E-selectin was observed in renal parenchyma and glomeruli. The angiotensin-converting enzyme 2 (ACE2) was detected in the proximal tubules but not in other regions of ICR, STZ-ICR, or LPS-ICR. In LPS-STZ ACE2 was detected both in renal tubules as well as in glomeruli. The Mac-1 and podoplanin-positive cells increased in the renal parenchyma with diabetic condition and there was the distribution of a large number of Mac-1-positive cells in LPS-STZ.</br>
Conclusions</br>
The Pg-LPS may induce diabetic renal inflammation such as glomerulosclerosis and tubulitis with infiltration of Mac-1/podoplanin positive macrophages via glomerular overexpression of VCAM-1 and E-selectin, resulting in accumulation of both ACE2 and FGF23 which were unmetabolized with the inflammation-induced kidney damage under the diabetic condition. Periodontitis may be a critical factor in the progress of nephropathy in diabetic patients.
en-copyright=
kn-copyright=

en-aut-name=KajiwaraKoichiro
en-aut-sei=Kajiwara
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SawaYoshihiko
en-aut-sei=Sawa
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FujitaTakahiro
en-aut-sei=Fujita
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TamaokiSachio
en-aut-sei=Tamaoki
en-aut-mei=Sachio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Department of Oral Growth & Development, Fukuoka Dental College
kn-affil=

affil-num=2
en-affil=Department of Oral Function & Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=3
en-affil=Department of Oral Growth & Development, Fukuoka Dental College
kn-affil=

affil-num=4
en-affil=Department of Oral Growth & Development, Fukuoka Dental College
kn-affil=
en-keyword=P. gingivalis
kn-keyword=P. gingivalis
en-keyword=LPS
kn-keyword=LPS
en-keyword=Diabetic nephropathy
kn-keyword=Diabetic nephropathy
en-keyword=VCAM-1
kn-keyword=VCAM-1
en-keyword=E-selectin
kn-keyword=E-selectin
en-keyword=ACE2
kn-keyword=ACE2
en-keyword=FGF23
kn-keyword=FGF23
END

start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=22
article-no=
start-page=e14640
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20201123
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=After parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (M Phi s) to the subepithelial stroma. These attracted M Phi s are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5 degrees C) and HS conditions (40.5 degrees C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p < .05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p < .05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p < .05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of M Phi s; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p < .05) attracted the M Phi s when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of M Phi s in the endometrium during the summer.
en-copyright=
kn-copyright=

en-aut-name=SakaiShunsuke
en-aut-sei=Sakai
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HatabuToshimitsu
en-aut-sei=Hatabu
en-aut-mei=Toshimitsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamamotoYuki
en-aut-sei=Yamamoto
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KimuraKoji
en-aut-sei=Kimura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=chemokine
kn-keyword=chemokine
en-keyword=cow
kn-keyword=cow
en-keyword=endometrial cells
kn-keyword=endometrial cells
en-keyword=endometritis
kn-keyword=endometritis
en-keyword=heat stress
kn-keyword=heat stress
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=4
article-no=
start-page=307
end-page=317
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=202008
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=D-Tagatose Effectively Reduces the Number of Streptococcus mutans and Oral Bacteria in Healthy Adult Subjects: A Chewing Gum Pilot Study and Randomized Clinical Trial
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We examined the effect of D-Tagatose on the growth of oral bacteria including Streptococcus mutans (S. mutans). Saliva collected from 10 healthy volunteers was plated on BHI medium (to culture total oral bacteria) and MBS medium (to culture S. mutans, specifically). Agar plates of BHI or MBS containing xylitol or D-Tagatose were cultured under aerobic or anaerobic conditions. We then counted the number of colonies. In BHI plates containing D-Tagatose, a complete and significant reduction of bacteria occurred under both aerobic and anaerobic conditions. In MSB medium, significant reduction of S. mutans was also observed. We then performed a doubleblind parallel randomized trial with 19 healthy volunteers. They chewed gum containing xylitol, D-Tagatose, or both for 4 weeks, and their saliva was collected weekly and plated on BHI and MSB media. These plates were cultured under anaerobic conditions. Total bacteria and S. mutans were not effectively reduced in either the D-Tagatose or xylitol gum group. However, S. mutans was significantly reduced in volunteers chewing gum containing both D-Tagatose and xylitol. Thus, D-Tagatose inhibited the growth of S. mutans and many types of oral bacteria, indicating that D-Tagatose intake may help prevent dental caries, periodontitis, and many oral diseases.
en-copyright=
kn-copyright=

en-aut-name=NagamineYuichi
en-aut-sei=Nagamine
en-aut-mei=Yuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HasibulKhaleque
en-aut-sei=Hasibul
en-aut-mei=Khaleque
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OgawaTakaaki
en-aut-sei=Ogawa
en-aut-mei=Takaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TadaAyano
en-aut-sei=Tada
en-aut-mei=Ayano
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KamitoriKazuyo
en-aut-sei=Kamitori
en-aut-mei=Kazuyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HossainAkram
en-aut-sei=Hossain
en-aut-mei=Akram
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YamaguchiFuminori
en-aut-sei=Yamaguchi
en-aut-mei=Fuminori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TokudaMasaaki
en-aut-sei=Tokuda
en-aut-mei=Masaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KuwaharaTomomi
en-aut-sei=Kuwahara
en-aut-mei=Tomomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MiyakeMinoru
en-aut-sei=Miyake
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

affil-num=1
en-affil=Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=2
en-affil=Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=3
en-affil=Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=4
en-affil=Department of Microbiology, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=5
en-affil=Department of Molecular Physiology and Biophysics, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=6
en-affil=Department of Molecular Physiology and Biophysics, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=7
en-affil=dDepartment of Medical Technology, Division of Fundamental Medical Technology, Ehime Prefectural University of Health Sciences
kn-affil=

affil-num=8
en-affil=eInternational Office, Kagawa University
kn-affil=

affil-num=9
en-affil=Department of Microbiology, Faculty of Medicine, Kagawa University
kn-affil=

affil-num=10
en-affil=Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kagawa University
kn-affil=
en-keyword=D-Tagatose
kn-keyword=D-Tagatose
en-keyword=xylitol
kn-keyword=xylitol
en-keyword=Streptococcus mutans
kn-keyword=Streptococcus mutans
en-keyword=oral bacteria
kn-keyword=oral bacteria
en-keyword=chewing gum
kn-keyword=chewing gum
END

start-ver=1.4
cd-journal=joma
no-vol=129
cd-vols=
no-issue=1
article-no=
start-page=9
end-page=15
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170403
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Does hydrogen-rich water really work?
kn-title=水素水は怪しい水でしょうか？
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=NakaoAtsunori
en-aut-sei=Nakao
en-aut-mei=Atsunori
kn-aut-name=中尾篤典
kn-aut-sei=中尾
kn-aut-mei=篤典
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=岡山大学大学院医歯薬学総合研究科　救急医学
en-keyword=水素水
kn-keyword=水素水
en-keyword=抗酸化作用
kn-keyword=抗酸化作用
en-keyword=抗炎症作用
kn-keyword=抗炎症作用
en-keyword=臨床応用
kn-keyword=臨床応用
en-keyword=疑似科学
kn-keyword=疑似科学
END

start-ver=1.4
cd-journal=joma
no-vol=67
cd-vols=
no-issue=6
article-no=
start-page=333
end-page=342
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201312
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Oncolytic Adenovirus-Induced Autophagy: Tumor-Suppressive Effect and Molecular Basis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Autophagy is a catabolic process that produces energy through lysosomal degradation of intracellular organelles. Autophagy functions as a cytoprotective factor under physiological conditions such as nutrient deprivation, hypoxia, and interruption of growth factors. On the other hand, infection with pathogenic viruses and bacteria also induces autophagy in infected cells. Oncolytic virotherapy with replication-competent viruses is thus a promising strategy to induce tumor-specific cell death. Oncolytic adenoviruses induce autophagy and subsequently contribute to cell death rather than cell survival in tumor cells. We previously developed a telomerase-specific replication-competent oncolytic adenovirus, OBP-301, which induces cell lysis in tumor cells with telomerase activities. OBP-301-mediated cytopathic activity is significantly associated with induction of autophagy biomarkers. In this review, we focus on the tumor-suppressive role and molecular basis of autophagic machinery induced by oncolytic adenoviruses. Addition of tumor-specific promoters and modification of the fiber knob of adenoviruses supports the oncolytic adenovirus-mediated autophagic cell death. Autophagy is cooperatively
regulated by the E1-dependent activation pathway, E4-dependent inhibitory pathway, and microRNA-dependent fine-tuning. Thus, future exploration of the functional role and molecular mechanisms underlying oncolytic adenovirus-induced autophagy would provide novel insights and improve the therapeutic potential of oncolytic adenoviruses.
en-copyright=
kn-copyright=

en-aut-name=TazawaHiroshi
en-aut-sei=Tazawa
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KagawaShunsuke
en-aut-sei=Kagawa
en-aut-mei=Shunsuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FujiwaraToshiyoshi
en-aut-sei=Fujiwara
en-aut-mei=Toshiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Center for Innovative Clinical Medicine, Okayama University Hospital

affil-num=2
en-affil=
kn-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=3
en-affil=
kn-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
en-keyword=oncolytic adenovirus
kn-keyword=oncolytic adenovirus
en-keyword=autophagy
kn-keyword=autophagy
en-keyword=E2F1
kn-keyword=E2F1
en-keyword=microRNA
kn-keyword=microRNA
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=6
article-no=
start-page=1527
end-page=1531
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Influence of Buffer on the Bacterial Respiratiration
kn-title=緩衝液の種類及び濃度と細菌の呼吸との関係
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The preparation of resting cells is one of the most important and most fundamental procedures for the physiological studies on bacteria. In order to know how we should do to get the resting cells suitable to the purposes of experiments, the author studied the influence of washing and suspending on the respiratory activity of bacteria. The results are summarized as follows: 1) For the purpose to obtain the resting cells of high respiratory activity, washing with phosphate buffer is better than that with distilied water or physiological saline solution. 2) When the prepared resting cells are used in a short time after the preparation, the respiratory activity is the same regardless of the sorts of the suspending solutions, distilled water, saline solution and phosphate buffer. 3) Borate and phthalate buffers of high concentration somewhat inhibit the respiration. However, in order to make the shift of pH less, M/15 (final concentration) is good. Phosphate buffer, even of M/15, does not inhibit the respiration. 4) In borate or phthalate buffers, the fall of respiratory activity is diminished by the addition of small amount of phospate.
en-copyright=
kn-copyright=

en-aut-name=YabeYoshiro
en-aut-sei=Yabe
en-aut-mei=Yoshiro
kn-aut-name=矢部芳郎
kn-aut-sei=矢部
kn-aut-mei=芳郎
aut-affil-num=1
ORCID=

en-aut-name=AkitaYoshimi
en-aut-sei=Akita
en-aut-mei=Yoshimi
kn-aut-name=秋田悦示
kn-aut-sei=秋田
kn-aut-mei=悦示
aut-affil-num=2
ORCID=

en-aut-name=AkitaKazuo
en-aut-sei=Akita
en-aut-mei=Kazuo
kn-aut-name=秋田和男
kn-aut-sei=秋田
kn-aut-mei=和男
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部微生物学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=2
article-no=
start-page=549
end-page=560
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Glutamic Acid Metabolism of Bacteria II: Glutamic acid metabolism of Staphylococcus albus
kn-title=細菌のグルタミン酸代謝に関する研究 第二篇 白色ブドウ球菌のグルタミン酸代謝
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=As to the physiological or metabolic features of pathogenic staphylococci, there are only a few reports, compared with those of Escherichia coli which was treated in report I. In this report, the author reports about the physiological aspects, particularly about the terminal respiratory system of Staphylococcus albus, entering from the studies of glutamic acid metabolism: 1) Staph. albus has the so-called citric acid cycle as its terminal respiratory ststem. 2) As a result of oxidative deamination, glutamic acid enters into the citric acid cycle and is further oxidized through this cycle. Glutamic acid is, however, best oxidized of all the intermediates of citric acid cycle and the related compounds. 3) Glutamic-aspartic and glutamic-alanic transaminations are carried out by this organism, in which glutamic acid plays the central role. 4) Divalent metal ions (Mg(++), Mn(++) and Fe(++)) show no remarkable effect on the glutamate-respiration of Staph. albus. 5) Of the various inhibitors tested, sodium azide, 2: 4-dinitrophenol, sodium arsenite and 8-hydroxyquinoline inhibit the glutamate-respiration strongly, and the most remarkable is the inhibitive action of 8-hydroxyquinoline. 6) Of the various antibiotics used, the inhibitive action of aureomycin is the most remarkable. Penicillin also shows some inhibitive action at pH 5.4. 7) The inhibition of the glutamate-respiration of this organism by these various inhibitors and antibiotics shows usually the tendency to rise up in the region of lower pH.
en-copyright=
kn-copyright=

en-aut-name=AkitaYoshimi
en-aut-sei=Akita
en-aut-mei=Yoshimi
kn-aut-name=秋田悦示
kn-aut-sei=秋田
kn-aut-mei=悦示
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=2
article-no=
start-page=541
end-page=547
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1957
dt-pub=19570228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Glutamic Acid Metabolism of Bacteria I: Glutamic acid metabolism of Escherichia coli communis and its application to the analysis of glutamic acid
kn-title=細菌のグルタミン酸代謝に関する研究 第一編 大腸菌のグルタミン酸代謝とその定量分析への応用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It is well known that glutamic acid, as well as aspartic acid, plays an important role in the metabolism of microorganisms. The author performed many experiments in order to study the physiological aspects of E. coli communis from the stand point of glutamic acid metabolism, and to apply it to the analysis of glutamic acid. The results were as follows: 1) E. coli can grow at any pH within the range from 5.4 to 8.0, but the growth is the best at pH 6.8 to 7.0. 2) The more remote from the optimum the cultural pH is, the nearer it comes to the optimum after the growth. This phenomenon is particularly remarkable on the acid side. 3) Using glutamic acid as substrate, the decarboxylation is chiefly carried out on the acid side, the deamination on the neutral or slightly alkaline side, and these two reactions are carried out simultaneously at pH 5.5 to 6.5. As for the optimum pH, pH 5.0 for decarboxylation and 7.0 for deamination. 4) The lower the cultural pH is, the higher the glutamic decarboxylase activity of the cultured organism becomes. The optimum reaction pH is, however, never shifted. 5) The E. coli, which was cultured in the pyridoxin-containing semi-synthetic medium, shows a very high activity to glutamic acid, but not to α-ketoglutaric acid, aspartic acid, alanine and pyruvic acid. 6) The aceton powder of the E. coli, which was cultured in the pyridoxin-containing semi-synthetic medium, still has the high glutamic decaboxylase activity. The optimum pH is from 4.4 to 5.1, where carbon dioxide is evolved quantitatively.
en-copyright=
kn-copyright=

en-aut-name=AkitaYoshimi
en-aut-sei=Akita
en-aut-mei=Yoshimi
kn-aut-name=秋田悦示
kn-aut-sei=秋田
kn-aut-mei=悦示
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=12
article-no=
start-page=4511
end-page=4520
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581231
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Physiological Studies on Drug-Resistant Bacteria 2. Physiological Study on Aureomycin-Resistant Escherichia coli
kn-title=抗生物質耐性菌の生理学的研究 第2編 オーレオマイシン耐性大腸菌の生理学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It has been reported that, in microorganisms, the acquisition of resistance to aureomycin is relatively difficult compared with the other antibiotics. Physiological studies on the aureomycin-resistant bacteria are also few as compared with those on the bacteria resistant to the other antibiotics. The author isolated a strain of Escherichia coli resistant to aureomycin, and studied on some physiological aspects of this strain in comparison with the sensitive strain. The results are summarized as follows: 1) In the culture of this aureomycin-resistant strain, there appeared a number of string-like cells, which might be considered to be produced by inhibition of cell division. No marked change could be observed in the characters of the colony and the staining of this strain. 2) Without addition of aureomycin, no difference was observed between the multiplication of this resistant strain and that of the sensitive stain. In the media containing aureomycin, however, the resistant strain showed a resistance to aureomycin about 10 to 100 times that of the sensitive strain. 3) In the absence of aureomycin, no marked difference existed between the respiratory activity of the resistant strain and that of the sensitive strain. In the presence of aureomycin, however, the respiratory activity of the resistant strain showed a resistance to aureomycin about 3 times that of the sensitive strain. 4) In the inhibitive action of aureomycin to the oxidation of lactate by the cell-free extract, no noticeable difference was observed between the resistant and the sensitive strains. These results suggest that the change of the cytoplasmic membrane of the resistant strain plays the most important role for exhibition of its high resistance to aureomycin.
en-copyright=
kn-copyright=

en-aut-name=HayashiSei
en-aut-sei=Hayashi
en-aut-mei=Sei
kn-aut-name=林生
kn-aut-sei=林
kn-aut-mei=生
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=12
article-no=
start-page=4501
end-page=4509
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1958
dt-pub=19581231
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Physiological Studies on Drug-Resistant Bacteria 1. Physiological Study on Streptomycin-Dependent Escherichia coli
kn-title=抗生物質耐性菌の生理学的研究 第1編 ストレプトマイシン依存性大腸菌の生理学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Drug-resistance of bacteria is one of the most important and also most troublesome problem in the chemotherapy of diseases. The author studied on some physiological aspects of streptomycin-dependent strain of Escherichia coli in comparison with streptomycin sensitive strain. The results are summarized as follows: 1) By inoculation of a great amount of streptomycin-sensitive E. coli on plain broth agar plates containing 1.000 γ / cc of streptomycin, one streptomycin-dependent strain was isolated. 2) The reverse mutant from this dependent strain was a streptomycin-sensitive strain, the re-reverse mutant from this reverse mutant was a dependent strain, and no resistant strain could be obtained. 3) This dependent stain could not multiply using streptomycin as the sole carbon or nitrogen source. 4) This dependent strain showed a higher requirement to streptomycin in the media containing the fatty acits related to the citric acid cycle than in those containing amino acids as its growth substrates. 5) Streptomycin showed no noticeable action on the respiration of this dependent strain. From these results, it is inferred that streptcomycin works not as a nutriment but as some working factor for the growth or multiplication of the streptomycin-dependent E. coli.
en-copyright=
kn-copyright=

en-aut-name=HayashiSei
en-aut-sei=Hayashi
en-aut-mei=Sei
kn-aut-name=林生
kn-aut-sei=林
kn-aut-mei=生
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=1
article-no=
start-page=277
end-page=282
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1959
dt-pub=19590131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Bacterial Metabolism by Means of Oxidation-Reduction Potential �T: Oxidation-Reduction Potential in the Culture Media of Salmonella typhi
kn-title=発育電位時間曲線による細菌代謝の研究 第1編 チフス菌による電位降下物質の研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to study some physiological aspacts of bacteria, using Sal. typhi 57S as the test organism, the author measured the oxidation-reduction potential of the culture media with the lapse time. Salt solution, bouillon and M/50 phosphate buffer were used as the fundamental culture media, and lactate and glucose as the substrates. The results were as follows: 1) The potential of the liquid media of Sal. typhi 57S is lower in longer time culture. By separation of bactera, this potential rapidly rises by about 60 to 70 % , and then becomes stable, though still shows the tendency of some rise. By re-pouring the separated bacteria into the original culture media, the potential falls again, and this fall is generally milder when the first culture time is shorter. 2) There are a symmetrical correlation between the increasing rate of growing bacteria and the rising rate of potential of the culture media by separation of bacteria. 3) In a medium, in which a collodion membrane is placed to separate the bacteria-containing and non-containing part, the fall of potential is more marked in the part far from the bacteria-containing part, and, on the contrary, the potential of the bacteria-containing part shows a rising tendency. This phenomenon is marked when the two parts are separated by cellophane or bladder membrane, and not so marked by filter paper.
en-copyright=
kn-copyright=

en-aut-name=UedaMaomi
en-aut-sei=Ueda
en-aut-mei=Maomi
kn-aut-name=植田真臣
kn-aut-sei=植田
kn-aut-mei=真臣
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=72
cd-vols=
no-issue=3
article-no=
start-page=889
end-page=899
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1960
dt-pub=19600228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Physiological Studies on the Metabolic Activities of Streptomycin-Resistant B. Dysentheriae. 1. The Carbohydrate Metabolism of Streptomycin-Resistant Sh. flexneri 2a
kn-title=ストレプトマイシン耐性赤痢菌の生理学的研究 第1編 ストレプトマイシン耐性赤痢菌駒込Ｂ�Vの糖代謝
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The resistance to streptomycin should be demonstrated by some differencies in metabolic reactions detectable by comparisons made between resistant and susceptible bacteria. This paper is concerned with the examination of the glucose metabolism of streptomycin-susceptible and streptomycin-resistant strains of Sh. flexneri 2a. The resistant strains was obtained by serial transfers in progressive levels of streptomycin. This resistant variant could withstand at least 10,000γ per ml of streptomycin. 1) The oxidative abilitys of the resistant strains is considerably less on pyruvate among the components of the citric acid cycle than is that of the susceptible strain. 2) Pyruvic acid, lactic acid, and acetic acid accumulates during the oxidation of glucose by resistant and susceptible strains. The amount of pyruvic acid produced from glucose by resistant strain is more than that by susceptible strain. In the rate of consumptions of glucose between resistant and susceptible strains there is much less difference. 3) No significant difference between both strains is detected in anaerobic metabolism of glucose. 4) Resistant strains which has grown on continued subculture by serial transfer in the medium not containing streptomycin possess still these properties. This culture was also found to be resistant to at least 10,000γ per ml of streptomycin. 5) It is suggested that streptomycin-resistant and susceptible strains dissimilates glucose mainly by way of Embden-Myerhof pathway, and also have Warbung-Dickens pathway as an alternate pathway. 6) Streptomycin-susceptible strain growes more rapidly than streptomycin-resistant strain in shaking culture. 7) These results suggests that streptomycin-resistant strain is more fermentative in the metabolism of glucose than streptomycin-susceptible strain, and a significant difference between streptomycin-resistant and streptomycin susceptible strains exists in pyruvate metabolism and its terminal respiration system.
en-copyright=
kn-copyright=

en-aut-name=KawaiKiyoshi
en-aut-sei=Kawai
en-aut-mei=Kiyoshi
kn-aut-name=川井潔
kn-aut-sei=川井
kn-aut-mei=潔
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=7
article-no=
start-page=1017
end-page=1023
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19650730
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Physiological Factors Participating on the Biosynthesis of Pyocianine by Pseudomonas aeruginosa
kn-title=緑膿菌産生色素 "Pyocianine" に関する研究―特にPyocianine生合成に影響する因子について―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Pseudomonas aeruginosa produces the two pigments, namely Pyocianine and Fluorescin. The Pyocianine is bluish green pigment and is derivative of phenazine pigment. In this paper, the physiological factors that give influences on the biosynthesis of Pyocianine by Pseudomouas aeruginosa in culture will be presented. 1. Pseudomonas aeruginosa required glycerine as a nitrogene source for the biosynthesis of Pyocianine. In a case that glucose was used as carbon source, the addition of magnesium ion was essential. 2. Within alanine, glutamate and aspartate on which the author examined, only alanine was of effective as nitrogen source for the Pyocianine synthesis. 3. The following results have been investigated by the studies on the atmospheric condition. Pseudomonas aeruginosa produced the pigment at a aerobic condition in the chemically satisfactory culture media, but could not produce at anaerobic condition in any culture media. 4. In the acidic media below pH 6.0, the growing of bacteria was very slight and the Pyocianine could not be produced. Moreover, the pigment did not be synthesized when the pH was descended rapidly, even if the startiag pH of the medium was neutral or slight basic. 5. The growing of the bacteria is indispensable for the synthesis of Pyocianine, then the resting cells could not synthesize the pigment.
en-copyright=
kn-copyright=

en-aut-name=SakanoSaizo
en-aut-sei=Sakano
en-aut-mei=Saizo
kn-aut-name=坂野才蔵
kn-aut-sei=坂野
kn-aut-mei=才蔵
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=77
cd-vols=
no-issue=4
article-no=
start-page=743
end-page=749
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1965
dt-pub=19650430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Physiological Aspects of Filamentous Elongated Bacteria Induced by X-ray Irradiation
kn-title=X線照射による異常長大菌の生理学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Sequences of cytological changes occurring in some bacteria following irradiation of X-ray have been described by many workers. It is known that, unless bacteria receive a considerable amount of irradiation, they continue to grow and become elongated, only their cell division being inbibited. In the present study, the author gave physiological aspects on the filamentous Salmonella typhi H901W elongated by culture following after X-ray irradiation (2000 r). 1. It was observed by Warburg's manometric technique that most of enzyme activities in the elongated cells were reduced. Especially, the induction of a adaptive enzyme for glucuronic acid could not be observed in the elongated cells. 2. In the post culture following after X-ray irradiation, protein and RNA increased as long as cell body elongates but DNA didn't increase. This result coincides with the finding that irradiated Salmonella typhi elongated without the increasing of number of nuclear apparatus. 3. In the studies on the agglutination reaction of the elongated cells, the author found that the agglutinability with antibody were reduced and moreover the isoelectric point moved slightly against to neutral pH.
en-copyright=
kn-copyright=

en-aut-name=NishiiSatoru
en-aut-sei=Nishii
en-aut-mei=Satoru
kn-aut-name=西井怜
kn-aut-sei=西井
kn-aut-mei=怜
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=1-2
article-no=
start-page=157
end-page=175
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19680228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Clinical and Experimental Study on Primary Lipoid Pneumonia
kn-title=コレステロール型肺炎の成因に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Clinicopathological and experimental studies were performed to clarify the genesis of primary lipoid pneumonia. Complete examination of resected lungs from six cases, which were believed to be suitable for this entity, were made. In addition to characteristic pathologic changes such as massive appearance of intralveolar foam cells, peribronchial and perivascular inflammation and interstitial fibrosis, definite changes of pulmonary arteries and veins of various size were noted. Pathologic changes in vessels were most prominent especially in the arteries of small and medium size. Definite narrowing of the lumen due to swelling of intima and thickening and swelling of both media and adventitia were noted. These changes were minimum in the large arteries. Occlusion due to thrombus was noted only in one case of medium sized artery. Based on these findings, experimental studies were undertaken to produce the similar pathologic changes in the lungs of dogs and rabbits. Experimental animals were divided into eight groups, where different experimental procedures were applied as follows. Ligation, to cause either occlusion or stenosis of pulmonary artery, vein or bronchus, was performed in five groups of dogs. Vasopression was injected daily for 30 successive days with the dose of 0.5cc. per kilogram of body weight in another group of rabbits. Intrapulmonary inoculation of Staphylococcus aureus was made with 0.3cc. of the bacterial suspension, containing 10mg. of the bacteria in 1cc. of physiologic saline, to induce pulmonary infection, directly through the thoracic wall. The other group of rabbit was fed daily with chow containing 1gm. of cholesterol for 30 days.
en-copyright=
kn-copyright=

en-aut-name=SatoMasahiko
en-aut-sei=Sato
en-aut-mei=Masahiko
kn-aut-name=佐藤雅彦
kn-aut-sei=佐藤
kn-aut-mei=雅彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学砂田外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=92
cd-vols=
no-issue=1-2
article-no=
start-page=73
end-page=83
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1980
dt-pub=19800228
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Characterization of carotenoid from Staphylococcus aureus and its physiological role
kn-title=黄色ブドウ球菌カロチノイド色素の分析化学的検討と生理的意義について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Although carotenoid is one of the typical pigments in Staphylococcus aureus, its properties and physiological role have not been well characterized. This paper examined carotenoid of S. aureus in regard to (1) factors affecting its production. (2) chemical properties and (3) its effect on liposomal permeability. (1) Carotenoid content increased during the late logarithmic growth phase. Production was potently accelerated by the addition of 10% NaCl (10 fold), 2% glycerin (10 fold) and 1% glycerolmonoacetate (250 fold). Oxygen was necessary but visible light did not effect the production. (2) The major component of carotenoid was C(30)Diaponeurosporenoic acid methyl ester. Minor components were Diaponeurosporene, Diapo-∫-carotene, Diapolycopenoic acid methyl ester, Diaponeurosporene ester and a hydroxyl derivative of Diaponeurosporene. Staphylococcal carotenoids were all C(30). (3) The addition of carotenoid to liposomes (made of staphylococcal phospholipids) increased the permeability for glycerol and glucose. This indicates that production of carotenoid may be an adaptational response of the bacteria.
en-copyright=
kn-copyright=

en-aut-name=SasaiKatsuko
en-aut-sei=Sasai
en-aut-mei=Katsuko
kn-aut-name=篠井加津子
kn-aut-sei=篠井
kn-aut-mei=加津子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部細菌学教室
en-keyword=黄色ブドウ球菌
kn-keyword=黄色ブドウ球菌
en-keyword=カロチノイド色素分析
kn-keyword=カロチノイド色素分析
en-keyword=Neurosporenoic acid
kn-keyword=Neurosporenoic acid
END

start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=2
article-no=
start-page=79
end-page=83
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2006
dt-pub=20060331
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Study on the method to quantify viable bacterial cellson the surface of endotracheal suction catheters
kn-title=気管内吸引カテーテルに付着した一般細菌の生菌数測定方法に関する検討
en-subtitle=
kn-subtitle=
en-abstract=As a method for the detection of viable bacteria attached to endotracheal suction catheters, we evaluated sonication and dissociation using a tube mixer. The catheter fragments with Pseudomonas aeruginosa PAO1 were treated by each of the two methods, and viable cells in the elutions were counted. The highest number of viable cells was observed at 0.5 min by either method. The viable cell
count decreased when the sonication time exceeded 1 min, while only a slight decrease of viable cells was observed by using a tube mixer. The catheters used for patients receiving care at home were fragmented and treated by a tube mixer to detach bacteria, and viable cells were counted. Electron microscopy observation showed an association between the viable cell count and morphology of surfaces of the catheters. These results suggest that adequate removal of bacteria attached to endotracheal suction catheters is possible by agitating catheter fragments for 0.5 min in physiological saline using a tube mixer.
kn-abstract=気管内吸引カテーテルに付着した一般細菌の生菌数測定方法について,超音波法およびチューブミキサーによ攪拌法を用いて検討した｡まず,人工的に緑膿菌を付着させた気管内吸引カテーテルを超音波処理することにより生菌数を測定した｡その結果,処理時間が1分を経過すると生菌数は減少をはじめ経時的に減少傾向を示した｡一方,攪拌法では0.5分の処理をピーク値としてその後の減少傾向は認められなかった｡次に,在宅療養患者に使用したカテーテルをチューブミキサーで0.5分攪拌後,生菌数の測定を行った｡また,同じカテーテルを用いて走査型電子顕微鏡による観察を行った結果,画面上の細菌数の印象と生菌数の測定結果に矛盾はなかった｡これらのことから気管内吸引カテーテルに付着した一般細菌の生菌数測定方法として,生理食塩水に入れたカテーテルをチューブミキサーで攪拌する方法が有用であると考えられた｡
en-copyright=
kn-copyright=

en-aut-name=InukaiMasako
en-aut-sei=Inukai
en-aut-mei=Masako
kn-aut-name=犬飼昌子
kn-aut-sei=犬飼
kn-aut-mei=昌子
aut-affil-num=1
ORCID=

en-aut-name=NomuraKayo
en-aut-sei=Nomura
en-aut-mei=Kayo
kn-aut-name=野村佳代
kn-aut-sei=野村
kn-aut-mei=佳代
aut-affil-num=2
ORCID=

en-aut-name=WatanabeKumi
en-aut-sei=Watanabe
en-aut-mei=Kumi
kn-aut-name=渡邉久美
kn-aut-sei=渡邉
kn-aut-mei=久美
aut-affil-num=3
ORCID=

en-aut-name=SendaYoshiko
en-aut-sei=Senda
en-aut-mei=Yoshiko
kn-aut-name=千田好子
kn-aut-sei=千田
kn-aut-mei=好子
aut-affil-num=4
ORCID=

en-aut-name=MitsuhataRitsuko
en-aut-sei=Mitsuhata
en-aut-mei=Ritsuko
kn-aut-name=光畑律子
kn-aut-sei=光畑
kn-aut-mei=律子
aut-affil-num=5
ORCID=

en-aut-name=KariyamaReiko
en-aut-sei=Kariyama
en-aut-mei=Reiko
kn-aut-name=狩山玲子
kn-aut-sei=狩山
kn-aut-mei=玲子
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部保健学科看護学専攻

affil-num=2
en-affil=
kn-affil=岡山大学医学部保健学科看護学専攻

affil-num=3
en-affil=
kn-affil=岡山大学医学部保健学科看護学専攻

affil-num=4
en-affil=
kn-affil=岡山大学医学部保健学科看護学専攻

affil-num=5
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科泌尿器病態学

affil-num=6
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科泌尿器病態学
en-keyword=気管内吸引カテーテル (Endotracheal suction catheter)
kn-keyword=気管内吸引カテーテル (Endotracheal suction catheter)
en-keyword=付着菌の解離 (removal of attached bacteria)
kn-keyword=付着菌の解離 (removal of attached bacteria)
en-keyword=生菌数測定方法 (viable cell counts)
kn-keyword=生菌数測定方法 (viable cell counts)
en-keyword=細菌学的評価 (microbiological evaluation)
kn-keyword=細菌学的評価 (microbiological evaluation)
en-keyword=形態学的評価 (morphological evaluation)
kn-keyword=形態学的評価 (morphological evaluation)
END

start-ver=1.4
cd-journal=joma
no-vol=88
cd-vols=
no-issue=1
article-no=
start-page=121
end-page=130
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1999
dt-pub=199902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Study on the Mechanism on Bacterial Adaptation to Deep-Sea Environment
kn-title=深海環境への微生物の適応機構に関する研究
en-subtitle=
kn-subtitle=
en-abstract=細菌は、外界の刺激に応答する機構を備えており、既に温度変化、浸透圧変化、放射線などの物理的刺激に応答した遺伝子発現調節機構が分子レベルで明らかにされている。深海から分離した耐圧性菌の耐圧機構を明らかにするため、細胞膜の脂肪酸組成と圧力の関係を検討した結果、加圧下で特異的な不飽和脂肪酸を増加させていることが明らかとなった。また、加圧下で培養した耐圧性細菌の細胞膜の酵素活性が大気圧下で培養したものよりも耐圧性になっていることを明らかにした。筆者らの単離した耐圧性菌の高圧下での特異的な脂肪酸の増加は、RNA合成阻害剤によって阻害されることから、細胞が圧力という刺激を感知し、その情報によって特異的な遺伝子の発現が引き起こされているものと推測された。この応答機構の分子的レベルでの解析によって、細菌の高圧環境への適応機構を明らかにする糸口が得られることが期待される。高圧環境下で細菌が生息するためには、細胞膜の機能だけでなくさまざまな酵素タンパク質や高分子合成系等の生化学的機能が、高圧環境下でも維持されていることが必要である。各種酵素タンパク質やタンパク質合成系の耐圧機構に関しては十分な生化学的解析がなされているとは言い難く、今後の研究課題として残されている。著者らは、加圧下での驚異的な脂肪酸の増加に、圧力に関連した遺伝子の発現が関与していることを示唆したが、既に加圧下で特異的に発現する遺伝子の存在が報告され、加圧下で特異的に発現する遺伝子が分離されている。また、圧力によって制御されるプロモーター部位も同定されている。圧力は、我々陸上環境に適応した生物にはなじみづらいが、海洋では極めて普遍的な物理的因子であり、その物理的因子を細菌がどのように認識し適応しているかを分子レベルで知ることは、海洋微生物資源の有効利用を図るときに非常に重要であると考えられる。今後、耐圧性細菌や好圧性細菌の加圧下における生理・生化学的な研究の進展によって、耐圧機構が分子レベルで明らかにされることが期待される。
kn-abstract=The world's oceans cover 70% of the eath's surface,with about 3,800m of average depth.Altough　the deep-sea environment with its high pressure and low temperatures is too extreme for most terrestrial and marine surface microorganisms,many barotolerant and barophilic bacteria have been found inhabiting the deep-sea.It is exyremely important for barophilic or barotolerand deep-sea bacteria to maintain the physiological functions of cytoplasmic membrane,which serves many vital functions.The fluidity of this cytoplasimic membrane composed of phospholipids and poteins is essential for the physiological functions of cells.As higher hydorstatic pressure raise the melting point of lipids and cause phase transition
of lipid under pressurs of up to 100MPa,barotolerant and barophilic bacrteria under high hydostatic pressure appear to regulate the composition of their membrane phospholipids. Therfore the characrization of cytoplasmic membrane under high pressure is indispensable to clarify the mecanisms of bacteria adaptation to the deep-sea enviroment.The effects of pressure and temperature acid compositon of barotolerant deep-sea bacteria were investigated.Deep-sea bacteria maintained their membrane fluidity by increasing the content of unique fatty acid in phospholipids under high hydrostatic pressure.Gene expression seems to be necessary for the synthesis of unique fatty under high hydrostatic pressure.
en-copyright=
kn-copyright=

en-aut-name=KamimuraKazuo
en-aut-sei=Kamimura
en-aut-mei=Kazuo
kn-aut-name=上村一雄
kn-aut-sei=上村
kn-aut-mei=一雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=deep-sea bacreria
kn-keyword=deep-sea bacreria
en-keyword=barotolerant bacteria
kn-keyword=barotolerant bacteria
en-keyword=fatty acid
kn-keyword=fatty acid
en-keyword=NADH oxidase
kn-keyword=NADH oxidase
END