The effects of heat-treatments around 1000°Ｃand subsequent annealing on the electrical properties of boron-doped silicon have been studied by electrical conductivity, Hall effect, and deep-level transient spectroscopy measurements. The high-temperature heat-treatments always induced net densities of donors. Four recovery stages, stages I-IV, of heat-treatment- induced donors were observed on isochronal annealing up to 400°Ｃ Conductivity changes in these stages can be explained as described below by the reactions of interstitial iron (Fei), its pair (Fe1Bs)with substitutional boron (Bs), and two unknown donors (D1, D2). That is, stage I (25°-100°Ｃ): D1→sink and Fei + Bs→FeiBs, stage II (100°-150°Ｃ)： FeiBs→Fei + Bs, stage III (200°-250°Ｃ):D2→sink, stage IV (250°-350°Ｃ)Fei→precipitation. Heat-treatments in an oxygen atmosphere greatly reduced the introduction of Fei and FeiBs in comparison with an argon atmosphere and mainly introduced D1 and D2 donors. The density of D2 was dependent on the heat-treatment temperature, while that of D1 showed almost no dependence. In stage I, D, was annihilated by first-order kinetics with an activation energy of 0.8 eV. It was indicated that DI and D2 have no relations to iron, copper, oxygen, nor carbon. Though their origins are still unidentified, there may be some interstitial impurities. In stage IV, Fei is suggested to precipitate at oxygen precipitates and dislocation loops formed by high-temperature heat-treatments. As to the application to iron gettering in the device fabrication process, it is proposed that annealing around 300°Ｃ is most suitable as the final heat-treatment step to remove iron and related defects from active regions of devices. Silicon wafers receive complex heat-treatments at various.
en-copyright= kn-copyright= en-aut-name=KamiuraY. en-aut-sei=Kamiura en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoF. en-aut-sei=Hashimoto en-aut-mei=F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YonetaM. en-aut-sei=Yoneta en-aut-mei=M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=silicon kn-keyword=silicon en-keyword=crystals kn-keyword=crystals en-keyword=doping kn-keyword=doping en-keyword=boron kn-keyword=boron en-keyword=heat treatment kn-keyword=heat treatment en-keyword=annealing kn-keyword=annealing en-keyword=electrical conductivity measurement kn-keyword=electrical conductivity measurement END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=1 article-no= start-page=55 end-page=62 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=201002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Differential Response of Heat-Shock-Induced p38 MAPK and JNK Activity in PC12 Mutant and PC12 Parental Cells for Differentiation and Apoptosis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Among the 3 mitogen-activated protein kinases -- ERK, p38 MAPK and JNK -- JNK has been suggested to participate in apoptosis, whereas p38 MAPK is thought to be part of the differentiation response. There are many common inducers of JNK and p38 MAPK, but the mechanisms underlying the differential response to apoptosis and differentiation are poorly understood. We found that heatshock activated p38 MAPK at 3min after exposure to a temperature of 44 in stress-hypersensitive PC12m3 mutant cells, while it activated JNK at 20min after the same heat treatment. However, heat shock activated p38 MAPK 5min after heat treatment and JNK 10min after heat treatment in PC12 parental cells. The extent of phosphorylation of p38 MAPK induced by heat shock in PC12m3 cells was significantly greater than that in PC12 parental cells, and a high level of heat-shock-induced neurite outgrowth was observed only in PC12m3 cells. On the other hand, heat-shock-induced JNK activation appeared more quickly and apoptosis started earlier in PC12 parental cells. These findings indicate that short stress induces p38 MAPK and longer stress induces JNK, and that the response of these kinases to heat shock differs depending on cell type.
en-copyright= kn-copyright= en-aut-name=MuraiHiroyasu en-aut-sei=Murai en-aut-mei=Hiroyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiragamiFukumi en-aut-sei=Hiragami en-aut-mei=Fukumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawamuraKenji en-aut-sei=Kawamura en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MotodaHirotoshi en-aut-sei=Motoda en-aut-mei=Hirotoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KoikeYoshihisa en-aut-sei=Koike en-aut-mei=Yoshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=InoueShigeki en-aut-sei=Inoue en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KumagishiKanae en-aut-sei=Kumagishi en-aut-mei=Kanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KanoYoshio en-aut-sei=Kano en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Occupational Therapy, School of Health Science, Kibi International University affil-num=2 en-affil= kn-affil=Department of Physical Therapy, School of Health Science, Kibi International University affil-num=3 en-affil= kn-affil=Department of Physical Therapy, School of Health Science, Kibi International University affil-num=4 en-affil= kn-affil=Department of Physical Therapy, School of Health Science, Kibi International University affil-num=5 en-affil= kn-affil=Department of Occupational Therapy, Faculty of Health and Welfare, Prefectural University of Hiroshima affil-num=6 en-affil= kn-affil=Department of Cellular and Molecular Biology, Research Institute of Health and Welfare, Kibi International University affil-num=7 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Occupational Therapy, School of Health Science, Kibi International University en-keyword=heat shock kn-keyword=heat shock en-keyword=neurite outgrowth kn-keyword=neurite outgrowth en-keyword=p38 MAP kinase kn-keyword=p38 MAP kinase en-keyword=JNK kn-keyword=JNK en-keyword=PC12 mutant cells kn-keyword=PC12 mutant cells END start-ver=1.4 cd-journal=joma no-vol=24 cd-vols= no-issue=1 article-no= start-page=49 end-page=64 dt-received= dt-revised= dt-accepted= dt-pub-year=1970 dt-pub=197002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Activation and isolation of mitochondrial adenosine triphosphatase by ultrasonic irradiation en-subtitle= kn-subtitle= en-abstract= kn-abstract=With the purpose to clarified the mode of localization and mechanisms of activation of ATPase in the mitochondrial membrane, analyses were made on the properties of mitochondrial ATPase from the structural and functional aspects. The activation of ATPase by DNP and Mg++ and the oligomycin sensitivity were investigated in a series of inner membrane fragment samples obtained by ultrasonic irradiation and those samples obtained in the processes of isolation and purification of ATPase from rat liver mitochondria and beef heart mitochondria in parallel with electron microscope observations. As a result it has been found that the membrane fragments obtained from rat liver and beef heart mitochondria by ultrasonication exhibited high respiratory activity and unmasked ATPase activity which was charac? terized by remarkable stimulation by Mg++ and inhibition by oligomycin and azide. Therefore, mitochondrial ATPase seems to be bound fairly closely to the inner mitochondrial membrane. In the membrane fragments prepared by ultrasonication of intact mitochondria, ATPase activity was stimulated by DNP, but in the supernatant fractions was not. On the other hand, the supernatant fraction obtained from BHM and inner membrane fragments by severe sonication exhibits a marked ATPase activity and the activity incresed in each step of the purification on the treatments with acid, protamine and heat. Especially in the case of membrane fragments the protamine treatment can be omitted. Electron microscope observation of the fractions in each step of the purification proved the head pieces to be ATPase. The ATPase activity of solubilized head pieces is insensitive to oligo. mycin and coincides with the soluble ATPase of PULLMAN etat. (8) in the points of its cold labile property and optimum pH, but it shown no accele. ration of ATPase activity by DNP.
en-copyright= kn-copyright= en-aut-name=YamamotoGoki en-aut-sei=Yamamoto en-aut-mei=Goki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsukamotoHiromichi en-aut-sei=Tsukamoto en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=24 cd-vols= no-issue=2 article-no= start-page=143 end-page=159 dt-received= dt-revised= dt-accepted= dt-pub-year=1970 dt-pub=197004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Isolation and some properties of oligomycin-sensitive adenosine triphosphatase from beef heart mitochondria and its morphological study en-subtitle= kn-subtitle= en-abstract= kn-abstract=1. To have a rapid isolation of oligomycin-sensitive ATPase particles (OSA particles), 0.1 mg DOC per mg of protein and 72 g potassium chlo. ride per I were added to mitochondria suspended in a tris.sucrose-histidine solution, which was followed by addition of 2-fold volume of chilled water, and fractionated by a discontinuous sucrose density gradient centrifugation. As a result, it was possible to reveal the OSA particle structure, composed of the head piece, stalk and thread-like structure of a superficial portion of the base pieces, stripped off from the mitochondrial inner membrane, in a layer of density.l.lO. This fraction exhibited a remarkable activity of ATPase sensitive to oligomycin, approximately 15 ,lJ.moles Pi released per mg of protein per minute at pH 8.6 at 37° in a non-ATP regenerating assay system, and contained almost no cytochromes. 2. When the OSA particles thus isolated were heated in water bath at 65° for 2 minutes, the head pieces were detached with a concomitant loss of oligomycin-sensitivity and were purified from the supernatant by precipitation with ammonium sulfate. 3. Trypsin in low concentration slightly induced a rise in the ATPase activity of OSA particles but in higher concentration it inhibited the activity. 4. OSA particles were resistant to the treatment of urea, and it was difficult to detach the head pieces by this treatment. 5. The some fraction obtained by solubilization of thc crude OSA particles with cholate and fractionation with ammonium sulfate exhibited ATPase activity in a masked form, and the ATPase activity with oligomycin. sensitivity was restored on addition of phospholipid. 6. A discussion was made on the mode of assembly of the head pieces and associated components and biochemical properties of OSA particles.
en-copyright= kn-copyright= en-aut-name=YamamotoGoki en-aut-sei=Yamamoto en-aut-mei=Goki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=46 cd-vols= no-issue=6 article-no= start-page=417 end-page=426 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=199212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Report of a study using phantom materials, and clinical experience with simultaneous radio-hyperthermotherapy. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Simultaneous radiohyperthermotherapy (SRH) is a combined hyperthermia-radiation therapy in which irradiation is given during heating. Mutual interference between the high energy radiotherapy system (Toshiba LMR-15A) and the 13.56 MHz capacitive heating system (Omron HEH-500C) was tested with phantom materials prior to a clinical trial with SRH. The energy and flatness of irradiation were not affected by the heating system within the range of clinical use. The high energy radiotherapy system did not affect the increase or distribution of temperature during simultaneous treatment. The results of this phantom study indicated that these apparatuses would not produce clinically significant mutual interference during SRH. A clinical trial was performed on a 57-year-old woman with postoperative recurrence of rectal cancer. This is the first reported clinical case treated with true SRH in which external irradiation was administered during mid capacitive heating. Twelve SRH treatments were performed on the recurrent lesion at a frequency of twice a week for six weeks using the apparatuses described above. There was a significant reduction in pain after treatment. The tumor marker carcinoembryonic antigen (CEA) level decreased after treatment. On CT images taken after treatment, the tumor site became a low density area which indicated necrosis. There were no side effects. These results suggest that further clinical study of SRH should be performed to clarify its advantages.
en-copyright= kn-copyright= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InamuraKeiji en-aut-sei=Inamura en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaharaSeiji en-aut-sei=Tahara en-aut-mei=Seiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MimuraSeiichi en-aut-sei=Mimura en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MikamiYasutaka en-aut-sei=Mikami en-aut-mei=Yasutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawasakiShoji en-aut-sei=Kawasaki en-aut-mei=Shoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=hyperthermia kn-keyword=hyperthermia en-keyword=capacitive heating kn-keyword=capacitive heating en-keyword=radiotherapy kn-keyword=radiotherapy en-keyword=phantom study kn-keyword=phantom study en-keyword=simultaneous radio-hyperthermotherapy kn-keyword=simultaneous radio-hyperthermotherapy END start-ver=1.4 cd-journal=joma no-vol=23 cd-vols= no-issue=3 article-no= start-page=219 end-page=226 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor factors of regional lymph node cells in the transplantation of Ehrlich ascites tumor cells. II. Properties of antitumor factors en-subtitle= kn-subtitle= en-abstract= kn-abstract=With the purpose to elucidate further the properties of the supernatant F4 obtained by centrifugation at 100, 000 g from the regional lymph node cells of the Cb mice sensitized with EHRLICH ascites tumor cells, the supernatant (cf. Report 13) was subjected to the following treatments:. The supernatant (F4) was first diluted variously with Hanks solution. 2. F4 was passed through Seitz filter. 3. Heated at 56°C for 30 minutes. 4. It was frozen and thawed. 5. Treated with O. 01 96 trypsin solution. Each of F4 frations so treated was used in the tissue culture of JTC-II cells (derived from EHRLICH cancer cells) as target cells. As a result we found that the antitumor factor passes th rough Seitz filter, and it loses its antitumor activity by 4-fold dilution or over. Likewise F4 loses its activity by freezing-thawing treatment as well as by trypsin treatment, while by heat treatment at 56°C for 30 minutes, it still retains its activity. From these finding, it is assumed that the antitumor factor contained in F4, fraction is not serum antibody but is a protein associated with the cell membrane.
en-copyright= kn-copyright= en-aut-name=NakashimaYouichi en-aut-sei=Nakashima en-aut-mei=Youichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=23 cd-vols= no-issue=3 article-no= start-page=209 end-page=218 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on the relationship between the function of reticuloendothelial system and the hematopoiesis. II. Experimental studies on 59Fe ferrokinetics in the induced hematological disorders of mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=The following conclusions were drawn from the ferrokinetic studies using 59Fe in mice, whose hematological disorders were induced by various treatments. 1. The ferrokinetics in the normal mice were studied. 2. Chloramphenicol (CP) administration in mice first induced ferrokinetics disturbances and then suppressed erythropoiesis. 3. Splenectomy induced hyper-erythropoiesis in the bone marrow, and CP administration after splenectomy suppressed this hyper-erythropoiesis. 4. Human gamma-globulin (H.G.G.) caused hypersplenism and a marked suppression of erythropoiesis in the bone marrow, and Chlorabulin administration suppresed erythropoiesis. Finally, the author has summarized the relationship of the RES function and hematopoiesis in mice as follows. 1. The spleen and liver reacted in the same manner with respect to the RES function to sequestrate 51Cr-labelled heat-damaged erythrocytes when hematological failures were induced. 2. The spleen and bone marrow reacted reversely with regard to the RES function. 3. When the RES function, especially that of the spleen was accentuated, the suppression of hematopoiesis was observed. 4. Chloramphenicol administration was followed by the suppressed hematopoiesis and the accentuated RES function. 5. Splenectomy accentuated the RES function in the bone marrow and liver, and also increased hematopoiesis in the bone marrow. 6. Human γ-globulin hypersensitization induced hyperfunction of the RES, especially of the spleen and suppression of the hematopoiesis.
en-copyright= kn-copyright= en-aut-name=HasegawaMakoto en-aut-sei=Hasegawa en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue=3 article-no= start-page=109 end-page=120 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=196706 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Inhibitory effect of guinea pig serum on JTC-11 cell growth in vitro en-subtitle= kn-subtitle= en-abstract= kn-abstract=The growth of JTC-11 cell line which was established from Ehrlich ascites carcinoma in vitro was inhibited by the addition of 2 per cent guinea pig serum to the control medium composed of 10 per cent bovine serum, 0.4 per cent lactalbumin hydrolysate and saline D. The concentration of guinea pig serum could be reduced to 0.02 per cent (lOγ of guinea pig serum protein/ml) with positive result, but 0.002 per cent guinea pig serum did not inhibit the growth at all. The inhibitory effect was not abolished by heating at 56°C, 66°C, and 70°C for 30 min but it was completely lost by heating at 100°C for 30 min. The inhibitory factor was undialyzable, and was inactivated with the treatment of 1mM HgCl2- Morphologically, the cells exposed to guinea pig serum showed pycnotic changes of the nuclei, accompanied by the formation of fine vacuole-like particles in the cytoplasm. Electron microscopic study revealed poor development of endoplasmic reticulum. There were more multivesicular bodies and large vacuoles with amorphous content in the cytoplasm of the damaged cells. The DNA synthesis in these cells was remarkably disturbed by 2 per cent guinea pig serum.
en-copyright= kn-copyright= en-aut-name=ChikataEiji en-aut-sei=Chikata en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UsuiKeiji en-aut-sei=Usui en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishizakiShizuo en-aut-sei=Nishizaki en-aut-mei=Shizuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KoshibaKimikazu en-aut-sei=Koshiba en-aut-mei=Kimikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TabuchiKatsusuke en-aut-sei=Tabuchi en-aut-mei=Katsusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NambaMasayoshi en-aut-sei=Namba en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=45 cd-vols= no-issue=5 article-no= start-page=339 end-page=345 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199110 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comparative antitumor activity of 5-fluorouracil and its prodrugs in combination with hyperthermia in vitro. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We investigated the antitumor activities of 5-fluorouracil (5-FU), 5'-deoxy-5-fluorouridine (5'-DFUR), 1-hexylcarbamoyl-5-fluorouracil (HCFU) and 1-(tetrahydro-2-furanyl)-5-fluorouracil (FT-207) in combination with hyperthermia in vitro. The antitumor effect of 5-FU (10(-4) M) was slightly enhanced by combination with hyperthermia (42 degrees C) for 2h, and the effect was determined to be additive. Synergistic enhancement of antitumor activity was obtained by the concurrent use of hyperthermia (42 degrees C, 2h) and 5'-DFUR (10(-4) M) or HCFU (10(-5) M). However, the antitumor effect of FT-207 (10(-4) M) in combination with hyperthermia was comparable that of hyperthermia alone. The synergistic enhancement of antitumor activity was not obtained for all drugs when the cells were preheated at 42 degrees C for 2h. On the other hand, when cells were pretreated with drugs before heat exposure, weak interactions were obtained after 5-FU and 5'-DFUR treatment, and a synergistic interaction was obtained after HCFU treatment. It is speculated that the metabolites of 5'-DFUR and HCFU enhance the cytotoxicity of 5-FU, or might change the threshold concentration for a cytotoxic effect of 5-FU in cancer cells.
en-copyright= kn-copyright= en-aut-name=ShiikiSigeo en-aut-sei=Shiiki en-aut-mei=Sigeo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FuchimotoSadanori en-aut-sei=Fuchimoto en-aut-mei=Sadanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkazaiYoshihiro en-aut-sei=Akazai en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsubaraNagahide en-aut-sei=Matsubara en-aut-mei=Nagahide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WatanabeTetsuya en-aut-sei=Watanabe en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=hyperthermia kn-keyword=hyperthermia en-keyword=5-fluorouridine kn-keyword=5-fluorouridine en-keyword=5'-deoxy-5-fluorouridine kn-keyword=5'-deoxy-5-fluorouridine en-keyword=1-hexylcarbomoyl-5-fluorouracil kn-keyword=1-hexylcarbomoyl-5-fluorouracil en-keyword=FT-207 kn-keyword=FT-207 END start-ver=1.4 cd-journal=joma no-vol=33 cd-vols= no-issue=2 article-no= start-page=73 end-page=80 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Co-existence of inhibitory and stimulatory factors modulating cell proliferation in rat liver cytoplasm. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Factors that inhibit and stimulate cell proliferation were found to coexist in rat liver supernatant. The inhibitory and stimulatory factors were separated by ethanol fractionation. Both factors were sensitive to heat- and trypsin-treatment. The activity of the inhibitor was diminished by partial hepatectomy. The inhibitor from normal livers inhibited DNA and RNA synthesis in the L-cell system, but the same fraction from regenerating livers caused little or no inhibition of nucleic acid synthesis. The stimulatory factor from regenerating livers had a stronger effect on cell proliferation than that of normal livers. Furthermore, the inhibitor from normal livers depressed DNA synthesis in vivo in regenerating livers. en-copyright= kn-copyright= en-aut-name=HataseOsamu en-aut-sei=Hatase en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujiiToshitake en-aut-sei=Fujii en-aut-mei=Toshitake kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KuramitsuMakoto en-aut-sei=Kuramitsu en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ItanoToshifumi en-aut-sei=Itano en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakahashiFumio en-aut-sei=Takahashi en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurakamiTetsuhide en-aut-sei=Murakami en-aut-mei=Tetsuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NishidaIsamu en-aut-sei=Nishida en-aut-mei=Isamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=growth factors kn-keyword=growth factors en-keyword=growth stimulants kn-keyword=growth stimulants en-keyword=growth inhibitants kn-keyword=growth inhibitants en-keyword=rat liver cytoplasm kn-keyword=rat liver cytoplasm en-keyword=cell proliferation kn-keyword=cell proliferation END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=1 article-no= start-page=25 end-page=30 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Reduction of ischemic damage by application of insulin-like growth factor-1 in rat brain after transient ischemia. en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to investigate a possible effect of insulin-like growth factor-1 (IGF-1) on ischemic brain injury, IGF-1 was applied topically on the brain surface of reperfused rat brain after 60 min of transient middle cerebral artery occlusion. In contrast to the cases treated with vehicle, the infarct volume was greatly reduced at 24 h of reperfusion by the treatment with IGF-1. Immunohistochemical analysis in the middle cerebral artery territory showed that Caspase-3 staining was markedly reduced in the cases with IGF-1 treatment, but 72-kDa heat shock protein staining remained almost unchanged. The present results suggest that treatment with IGF-1 exerts a significant effect on ameliorating brain injury after transient focal brain ischemia. Moreover, this effect is greatly associated with the reduction of Caspase-3 staining, but is only minimally associated with a decreasd stress response at the cellular level.
en-copyright= kn-copyright= en-aut-name=WangJiang Ming en-aut-sei=Wang en-aut-mei=Jiang Ming kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HayashiTakeshi en-aut-sei=Hayashi en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ZhangWen Ri en-aut-sei=Zhang en-aut-mei=Wen Ri kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=LiFeng en-aut-sei=Li en-aut-mei=Feng kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IwaiMasanori en-aut-sei=Iwai en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AbeKoji en-aut-sei=Abe en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=caspase-3 kn-keyword=caspase-3 en-keyword=cerebra? ischemia kn-keyword=cerebra? ischemia en-keyword=72-KDa heat shock protein kn-keyword=72-KDa heat shock protein END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=4 article-no= start-page=145 end-page=151 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A novel multicolor immunofluorescence method using heat treatment. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We describe a novel method for immunofluorescent detection of multiple antigens in a single paraffin-embedded tissue section. We hypothesized that if fluorescent dyes are resistant to heat treatment, then thermal inactivation of immunoglobulins during antigen detection procedures might make it possible to use multicolor immunofluorescence detection even if the primary antibodies are from the same species. We found that several fluorescent dyes, including fluorescein isothiocyanate (FITC), Cy3 and Cy5, were resistant to heating at 90 degrees Celsius for 15 min, whereas the antigenicities of the primary antibodies were lost completely. This novel method, which uses heat treatment between staining steps, has great advantages for multicolor immunofluorescence because unlabeled primary antibodies from the same species can be used. Therefore, by using this method not only 3 unlabeled mouse monoclonal antibodies but also 3 unlabeled rabbit antisera can be used as primary antibodies for multicolor immunofluorescence.
en-copyright= kn-copyright= en-aut-name=SuzukiTakao en-aut-sei=Suzuki en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeGenshu en-aut-sei=Take en-aut-mei=Genshu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IkedaKatsuhide en-aut-sei=Ikeda en-aut-mei=Katsuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MitsuyaToshiyuki en-aut-sei=Mitsuya en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Showa University affil-num=2 en-affil= kn-affil=Showa University affil-num=3 en-affil= kn-affil=Showa University Fujigaoka Hospital affil-num=4 en-affil= kn-affil=Showa University en-keyword=multicolor immunofluorescence kn-keyword=multicolor immunofluorescence en-keyword=heat inactivation kn-keyword=heat inactivation en-keyword=confocal laser scanning microscope kn-keyword=confocal laser scanning microscope END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=3 article-no= start-page=169 end-page=174 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=199306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hyperthermotherapy added to the multidisciplinary therapy for penile cancer. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We performed a long-term follow-up of 4 patients with penile cancer who underwent hyperthermotherapy from August 1985 until August 1992. Hyperthermia was applied using a frequency of 350 MHz with a waveguide applicator twice a week for 60 min each for an average of 9.5 times (varying from 6 to 13 times). The total heating time that the temperature of urethra could be kept above 42 degrees C, was 166 min on the average (ranging from 0 to 463 min). Two patients classified as stage I according to the Jackson classification and 1 patient classified as stage IV underwent combined radiotherapy and received an average radiation dose of 53 Gy (range, 40-70 Gy). Among these patients 2 underwent combined chemotherapy with bleomycin or peplomycin. Malignant cells disappeared posttherapeutically and in August 1992, after an average of 5 years and 9 months (varying from 4 years 6 months to 6 years 10 months), the patients were free of recurrences. The one patient on stage IV had extensive invasion of the abdominal wall, but still recovered completely. One patient on stage III underwent combined chemotherapy and hyperthermotherapy, but heating had obviously been insufficient. There was a residue of malignant cells after the treatment and we performed a penectomy. Regarding functional preservation of the penis a multidisciplinary therapy incorporating hyperthermotherapy can be expected to increase the curativity. This indicates that it could induce in an advanced case, where an operation would be difficult, complete remission.
en-copyright= kn-copyright= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsushimaTomoyasu en-aut-sei=Tsushima en-aut-mei=Tomoyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NasuYasutomo en-aut-sei=Nasu en-aut-mei=Yasutomo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AsaumiJunichi en-aut-sei=Asaumi en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishikawaKoji en-aut-sei=Nishikawa en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GaoXian Shu en-aut-sei=Gao en-aut-mei=Xian Shu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=JojaIkuo en-aut-sei=Joja en-aut-mei=Ikuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakedaYoshihiro en-aut-sei=Takeda en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TogamiIzumi en-aut-sei=Togami en-aut-mei=Izumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=MakihataEiichi en-aut-sei=Makihata en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KawasakiShoji en-aut-sei=Kawasaki en-aut-mei=Shoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=OhmoriHiroyuki en-aut-sei=Ohmori en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama Univresity affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University affil-num=11 en-affil= kn-affil=Okayama University affil-num=12 en-affil= kn-affil=Okayama University affil-num=13 en-affil= kn-affil=Okayama University en-keyword=penile cancer kn-keyword=penile cancer en-keyword=hyperthermia kn-keyword=hyperthermia en-keyword=radiotherapy kn-keyword=radiotherapy en-keyword=chemotherapy kn-keyword=chemotherapy END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=4 article-no= start-page=249 end-page=254 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=199308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hyperthermotherapy for postoperative local recurrences of rectal cancer. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Between November 1984 and August 1992 we used hyperthermotherapy in six cases of local recurrence of rectal cancer. Hyperthermotherapy was performed on the average 8.7 times (range: 3-18) for each patient for 60 min each. All patients underwent combined radiotherapy and received a mean radiation dose of 42.5 Gy (range: 9-60 Gy). Five patients underwent heating within 1 h after irradiation and one patient simultaneously with the irradiation. Four patients underwent combined chemotherapy and two patients immunotherapy. Before the treatment all patients had painful lesions, but pain decreased posttherapeutically in five patients. Performance status improved in two patients. High carcinoembryonic antigen levels prior to the therapy in four patients decreased in all cases after treatment. Posttherapeutical computed tomograms revealed only minor response or no changes. After the treatment, four patients died of exacerbations of recurrent tumors and one patient of distant metastases. The patient who underwent simultaneous radiohyperthermotherapy is presently alive, in August 1992, 38 months after initiation of the treatment. The 50% survival time after initiation of the treatment was 25 months (range: 10-38 months). Hyperthermotherapy combined with radiotherapy, chemotherapy and/or immunotherapy was useful for the alleviation of pain in patients who developed local recurrence after surgery, and improved survival after recurrences can be expected.
en-copyright= kn-copyright= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HizutaAkio en-aut-sei=Hizuta en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MakihataEiichi en-aut-sei=Makihata en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AsaumiJunichi en-aut-sei=Asaumi en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NishikawaKoji en-aut-sei=Nishikawa en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=GaoXian Shu en-aut-sei=Gao en-aut-mei=Xian Shu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakagawaTomio en-aut-sei=Nakagawa en-aut-mei=Tomio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TogamiIzumi en-aut-sei=Togami en-aut-mei=Izumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TakedaYoshihiro en-aut-sei=Takeda en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=JojaIkuo en-aut-sei=Joja en-aut-mei=Ikuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=KawasakiShoji en-aut-sei=Kawasaki en-aut-mei=Shoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University affil-num=11 en-affil= kn-affil=Okayama University affil-num=12 en-affil= kn-affil=Okayama University affil-num=13 en-affil= kn-affil=Okayama University affil-num=14 en-affil= kn-affil=Okayama University en-keyword=rectal cancer kn-keyword=rectal cancer en-keyword=local recurrence kn-keyword=local recurrence en-keyword=hyperthermia kn-keyword=hyperthermia en-keyword=radiotherapy kn-keyword=radiotherapy en-keyword=chemotherapy kn-keyword=chemotherapy END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=311 end-page=318 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Binding Specificities of Lectins to Immobilized Glycoproteins and Oligosaccharides Differ from Those of Immobilized Lectins to Oligosaccharides en-subtitle= kn-subtitle= en-abstract= kn-abstract=The carbohydrate-binding specificities of lectins in solution to glycoproteins and neoglycolipids immobilized on a solid phase were analyzed in order to establish a simple, rapid method for structural analysis of the carbohydrate moieties of small amounts of individual glycoproteins blotted on membrane. Eight glycoproteins containing typical O-linked tetrasaccharides or a series of typical N-linked oligosaccharides of the high-man-nose type, hybrid type, and complex type and 6 neoglycoproteins containing mono- or di-saccharides were dot blotted on membranes and the membranes were then reacted with 8 kinds of horseradish peroxidase-conjugated lectins before and after heat treatment. Neoglycolipids containing the glycoprotein-derived oligosaccharides immobilized on a thin layer chromatography plate were also reacted with lectins. The heat treatment of the membrane increased lectin reactivity toward the glycoproteins. The carbohydrate-binding behavior of lectins, Phaseolus vulgaris erythroagglutinin, wheat germ agglutinin, and concanavalin A in solution toward glycoproteins and neoglycolipids immobilized on a solid phase differed from that of immobilized lectins toward oligosaccharides in solution. This difference should be noted in lectin detection of specific carbohydrates of individual glycoproteins on membrane.
en-copyright= kn-copyright= en-aut-name=TangWei en-aut-sei=Tang en-aut-mei=Wei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiuraTakehiko en-aut-sei=Miura en-aut-mei=Takehiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakataMunehiro en-aut-sei=Nakata en-aut-mei=Munehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KojimaNaoya en-aut-sei=Kojima en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MizuochiTsuguo en-aut-sei=Mizuochi en-aut-mei=Tsuguo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Tokai University affil-num=2 en-affil= kn-affil=Tokai University affil-num=3 en-affil= kn-affil=Tokai University affil-num=4 en-affil= kn-affil=Tokai University affil-num=5 en-affil= kn-affil=Tokai University en-keyword=glycoprotein kn-keyword=glycoprotein en-keyword=lectins kn-keyword=lectins en-keyword=lectin binding specificity kn-keyword=lectin binding specificity en-keyword=neoglycolipid kn-keyword=neoglycolipid en-keyword=oligosaccharide kn-keyword=oligosaccharide END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue=1 article-no= start-page=15 end-page=26 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on prevention of infection (II) The enzymologic traits and pro?tective ability of the fractions obtained from Sal. typhi by high speed centrifugation en-subtitle= kn-subtitle= en-abstract= kn-abstract=By physically destroying typhoid bacilli and centrifuging at a high speed, an insoluble granular fraction (P1) and soluble fraction (S1) were obtained. Chemical and enzymologic properties of these substances as well as their influences on the protective ability against infection were studied; and the following results were attained: 1. P1 contains an extremely small amount of proteins when compared with S1. 2. The enzymologic activity of P1 is entirely different from that of S1. In P1 the respiratory enzyme system of only lactate and succinate is localized. 3. Although both P1 and S1 possess the antibody-producing ability in serum of rabbit to the same high degree, P1 imparts to mice a markedly high protective ability against infection. 4. By the heat-treatment of P1 its antigenicity is lost at the same time.
en-copyright= kn-copyright= en-aut-name=MurakamiSakae en-aut-sei=Murakami en-aut-mei=Sakae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshiokaTatsuji en-aut-sei=Yoshioka en-aut-mei=Tatsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkaYoshikazu en-aut-sei=Oka en-aut-mei=Yoshikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MatsuuraYoshiyuki en-aut-sei=Matsuura en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=4 article-no= start-page=211 end-page=216 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A new capacitive heating applicator for the simultaneous radiohyperthermotherapy of superficial and shallow-seated tumors. en-subtitle= kn-subtitle= en-abstract= kn-abstract=External capacitive heating is the usual method of electromagnetic wave heating, in which the tumor is caught and heated between two opposite applicators. Using a phantom, the authors developed and evaluated the performance of a new capacitive heating applicator designed for simultaneous radiohyperthermotherapy (SRH) in which the electron beam irradiation is provided from above an external capacitive heating applicator for the treatment of superficial and shallow-seated tumors. The trial applicator was constructed to fulfill the following conditions: 1. use of an electrode plate which does not affect the electron beam depth dose, 2. a uniform thickness to maintain flatness of the electron beam, and 3. a cooling function to prevent damage to normal skin tissue and enhance the therapeutic gain factor. This applicator was comprised of a 0.1-mm-thick copper electrode and a 5-mm-thick cooling chamber. The depth of the 80% dose of the new applicator was 21 mm with a 9-MeV electron beam and 36mm with a 15-MeV electron beam, which was comparable to the effect of a conventional irradiation bolus. The temperature distribution produced by the trial applicator was symmetrical on both sides from the center of the applicator. The 50% specific absorption rate region was 6.4 cm wide at a depth of 1 cm from the phantom surface and 2.8 cm wide at a depth of 3 cm. There have been no previous reports on the development of an external capacitive heating applicator designed for the SRH of superficial and shallow-seated tumors; this is the first such report.(ABSTRACT TRUNCATED AT 250 WORDS)
en-copyright= kn-copyright= en-aut-name=TanakaAkio en-aut-sei=Tanaka en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InamuraKeiji en-aut-sei=Inamura en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KawasakiShouji en-aut-sei=Kawasaki en-aut-mei=Shouji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=simultaneous radiohyperthermotherapy kn-keyword=simultaneous radiohyperthermotherapy en-keyword=applicator kn-keyword=applicator en-keyword=capacitive heating kn-keyword=capacitive heating en-keyword=superficial and shallowseated tumor kn-keyword=superficial and shallowseated tumor END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=1 article-no= start-page=49 end-page=52 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Type IV collagen-degrading enzyme activity in human serum. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Type IV collagen-degrading enzyme activity was detected in human serum. Serum was preincubated with 4-aminophenylmercuric acetate and trypsin to activate the enzyme prior to assay. Type IV collagen, purified from human placentas and radiolabeled with [1-14C] acetic anhydride, was used as the substrate. The enzyme activity was measured at pH 7.5 and inhibited by treatment with ethylenediaminetetraacetic acid or heat. The assay of type IV collagen-degrading enzyme in human serum might be useful for estimating the degradation of type IV collagen.
en-copyright= kn-copyright= en-aut-name=HashimotoNoriaki en-aut-sei=Hashimoto en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KobayashiMichio en-aut-sei=Kobayashi en-aut-mei=Michio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HigashiToshiro en-aut-sei=Higashi en-aut-mei=Toshiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=type IV collagen-degarading enzyme kn-keyword=type IV collagen-degarading enzyme en-keyword=collagen degradation kn-keyword=collagen degradation en-keyword=collagenase kn-keyword=collagenase en-keyword=type IV collagen kn-keyword=type IV collagen en-keyword=metalloproteinase kn-keyword=metalloproteinase END start-ver=1.4 cd-journal=joma no-vol=31 cd-vols= no-issue=1 article-no= start-page=71 end-page=80 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=COP, a new alloy for surgical implants en-subtitle= kn-subtitle= en-abstract= kn-abstract=Today Vitallium is used for surgical implants. It is a casting alloy which, with advances in casting technology, is also used commercially for making instruments of fairly complex shape. Because of its expense, however, it is not widely used in Japan. Instead, a series of 18-8 Mo alloys are used in Japan even though of insufficient strength. Used over a long period of time in the body, especially for the purpose of preserving structual functions as part of the human skeleton, it often corrodes, resulting in either abnormalities in tissue cells or, because of its insufficient strength, danger of bending and breaking with aging. In spite of a marked advance in fracture treatment, we have hardly any suitable materials for making instruments appropriate to the internal fixation of fractures in Japan. We, therefore, conducted various experiments to develop an alloy with sufficient corrosive resistance and strength that could be formed into a complex shape to take the place of Vitallium alloy, finally succeeding in developing an alloy we call "COP". The characteristic properties of COP may be summarized as follows: 1. The main components are 20% Cr, 20% Ni, 20% Co and 4% Mo aside from 0.2% P. 2. As it contains "P", it shows a marked age-hardening. In its molten state its machinability is excellent, and later it can readily be hardened by heat-treatment. 3. It has not only a marked yield point and tensile strength but also has toughness in elongation and reduction of area, showing a strength which surpasses Vitallium. 4. Its corrosive resistance is great. 5. Its cost is far cheaper than Vitallium.
en-copyright= kn-copyright= en-aut-name=SunamiYoshifumi en-aut-sei=Sunami en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshikawaEijiroo en-aut-sei=Ishikawa en-aut-mei=Eijiroo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Tokushu Seiko Co. Ltd. END start-ver=1.4 cd-journal=joma no-vol=36 cd-vols= no-issue=1 article-no= start-page=1 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=198202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Factors inhibiting cell proliferation in rat liver cytoplasm. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Two factors from normal rat liver cytoplasm inhibited the proliferation of cultured L-929 fibroblasts. One was arginase, the other was a small molecular weight inhibitor stable to trypsin and heat treatment. The small molecular weight inhibitor inhibited the protein and DNA synthesis of L-cells. Inhibition of DNA synthesis was thought to be secondary to the inhibition of protein synthesis.
en-copyright= kn-copyright= en-aut-name=KuramitsuMakoto en-aut-sei=Kuramitsu en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuiHideki en-aut-sei=Matsui en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TokudaMasaaki en-aut-sei=Tokuda en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HataseOsamu en-aut-sei=Hatase en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Kochi Medical College affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Kagawa Medical School en-keyword=cell proliferation kn-keyword=cell proliferation en-keyword=growth factor kn-keyword=growth factor en-keyword=inhibiting factor kn-keyword=inhibiting factor en-keyword=rat liver cytosol kn-keyword=rat liver cytosol en-keyword=L-cells kn-keyword=L-cells END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=239 end-page=250 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198406 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Prostacyclin and thromboxane in cerebral vasospasm II: Effects of thromboxane synthetase inhibitor (OKY-1581) on experimentally-induced cerebral vasospasm en-subtitle= kn-subtitle= en-abstract= kn-abstract=OKY-1581, a thromboxane A2 (TXA2) synthetase inhibitor, was administered to cats with normal and constricted basilar arteries. At a dose of 60mg/kg (i.v.), both normal and constricted vessels dilated, and the mean arterial blood pressure (MABP) fell from 55 to 75 mmHg. If MABP remained constant, vessel diameter did not change. Subarachnoid hemorrhage (SAH) was simulated by intracisternal injection of autologous arterial blood. Regional cerebral blood flow (rCBF) was assessed by the heat clearance and H2 clearance methods. The two methods presented similar response profiles. rCBF responses to intravenous OKY-1581 fell into 3 categories: A) no change in rCBF, B) decrease in rCBF related to MABP and C) increase in rCBF in the presence of hypotension. Types A and B were observed in 3 out of 10 control cats and 4 out of 14 SAH-induced cats, with Type C responses in the remainder. There was no significant difference between the groups. While the results do not support a major role for TXA2 in cerebral vasospasm pathogenesis, OKY-1581 may still be useful in the treatment of cerebral vasospasm, as it improves distal and deep circulation and inhibits platelet aggregation.
en-copyright= kn-copyright= en-aut-name=YabunoNobuyoshi en-aut-sei=Yabuno en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=cerebral vasospasm kn-keyword=cerebral vasospasm en-keyword= thromboxane A2 kn-keyword= thromboxane A2 en-keyword=OKY-1581 kn-keyword=OKY-1581 END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue= article-no= start-page=45 end-page=56 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19931215 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Study of Deactivating Methods against Ceder Pollenosis Antigen in Vitro kn-title=「スギ花粉症」抗原性物質の不活性化に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Study of deactivating methods against ceder pollenosis antigen in vitro: Every spring season, there are numerous pollenosis patients, especially ceder pollenosis, in our country. However, the radical theraputics against the pollenosis has not been established yet. Some deactivating methods, ultraviolet (UV) irradiation, wet heat treatment and adsorption method to the extracted solution of ceder pollen were examined. Deactivating effect against the pollenosis antigen was determined by measuring the changes of the specific antibody values (IgE antibody) in patient blood serum as the marker in vitro. It was cleared that the UV irradiation, the heat treatment over 60℃ and the adsorbent containing the acid site were effective on the deactivation against the antigen. en-copyright= kn-copyright= en-aut-name=TakashimaSeisuke en-aut-sei=Takashima en-aut-mei=Seisuke kn-aut-name=高島征助 kn-aut-sei=高島 kn-aut-mei=征助 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学地域共同研究センター END start-ver=1.4 cd-journal=joma no-vol=29 cd-vols= no-issue= article-no= start-page=11 end-page=15 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200710 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Material recycling of blast furnace slag by phase separation of glass kn-title=ガラスの分相現象を利用した高炉水砕スラグの再資源化技術の開発 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A novel recycling process of blast furnace slag was developed in order to obtain colorless silica−rich solids by using phase separation of borosilicate glass. B(2)O(3) was added to blast furnace slag to promote the phase separation. The slag glasses were heat-treated above glass transition temperatures. The slag glass prepared from blast furnace slag gelled after the heat treatment and the subsequent three types of acid treatment. The ratios of SiO(2) component in the gels were 40 - 60mass%. On the other hand, phase separation was observed on the surface of the slag glasses prepared from pre-treated slag by 2.5N HCI after the heat treatment. After soaking in acid, they did not gel and changed to insoluble colorless solids. According to compositional analyses, it was found that the insoluble colorless solids contained 70 - 90mass% SiO(2). No colored ions such as Cr, Mn, and Fe were confirmed in the remaining insolubles by optical absorption measurement. Therefore, the colorless silica-rich solids were successfully obtained in the present process. The end products obtained in the present process are expected as material of glass. en-copyright= kn-copyright= en-aut-name=SakidaShinichi en-aut-sei=Sakida en-aut-mei=Shinichi kn-aut-name=崎田真一 kn-aut-sei=崎田 kn-aut-mei=真一 aut-affil-num=1 ORCID= en-aut-name=MikamiSyuuhei en-aut-sei=Mikami en-aut-mei=Syuuhei kn-aut-name=三上修平 kn-aut-sei=三上 kn-aut-mei=修平 aut-affil-num=2 ORCID= en-aut-name=NanbaTokuro en-aut-sei=Nanba en-aut-mei=Tokuro kn-aut-name=難波徳郎 kn-aut-sei=難波 kn-aut-mei=徳郎 aut-affil-num=3 ORCID= en-aut-name=MiuraToshinari en-aut-sei=Miura en-aut-mei=Toshinari kn-aut-name=三浦嘉也 kn-aut-sei=三浦 kn-aut-mei=嘉也 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学保健環境センター環境安全部門 affil-num=2 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 affil-num=3 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 affil-num=4 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 en-keyword=Blast furnace slag kn-keyword=Blast furnace slag en-keyword=Recycling kn-keyword=Recycling en-keyword=Colorless silica-rich solids kn-keyword=Colorless silica-rich solids en-keyword=Phase separation kn-keyword=Phase separation en-keyword=Glass kn-keyword=Glass END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=1 article-no= start-page=7 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Characterization of Cystathionine γ-Synthase from Thermoacidophilic Archaea Sulfolobus tokodaii kn-title=好熱好酸性アーキア Sulfolobus tokodaii 由来シスタチオニン γ-シンターゼの精製及び性質検討 en-subtitle= kn-subtitle= en-abstract=好熱好酸性アーキア Sulfolobus tokodaii 由来シスタチオニンγﾝシンターゼ（stCGS）遺伝子を pET-11a に組み込み pET-stCGS を構築した．このベクターでE. coli Rosettaﾝgami（DE3）を形質転換し，本遺伝子を発現させ，精製及び性質検討を行った．大腸菌で発現したシスタチオニンγﾝシンターゼの活性が無細胞抽出液で確認できた．S. tokodaii シスタチオニンγﾝシンターゼを70℃熱処理 DEAEﾝトヨパールイオン交換カラム等により単一精製した．精製酵素の最適温度は100℃以上であり，熱安定性は60分間処理で70℃までほぼ100ｵの残存活性を示した．また，最適pHについてはリン酸緩衝液やブリトンﾝロビンソン広域緩衝液の場合はpH7.0の時が最も活性が高く，トリス塩酸緩衝液の場合はpH9.0が最適であった．pH安定性についてはpH5.0〜9.0において安定であった．O-ホスホ-l-ホモセリンに対するKm，Vmaxは，それぞれ0.82mM，2.42U/rであった．アポ酵素のホロ化実験により，本酵素活性がPLP に依存していることが明らかとなった．更に本酵素の脱離反応での基質特異性の検討を行った．変異酵素を用いた実験により，stCGSの基質特異性には，活性中心に存在するPhe97を含む領域が深く関わっていることが示唆された． kn-abstract=The gene encoding a cystathionine γ-synthase from Sulfolobus tokodaii was cloned and expressed in Escherihia coli Rosetta-gami (DE3). Cystathionine γ-synthase ［EC 2. 5. 1. 48］ from Sulfolobus tokodaii (stCGS) was purified by heat treatment, DEAE- Toyopearl 650M and Sephacryl S-300 column chromatographies from E. coli transformants. stCGS shows optimum activity at pH 7.0, and is stable between pH5.0 and pH9.0. The optimum temperature of stCGS is above 100℃, and the enzyme showed the remaining activity of almost 100% up to 70℃. The K(m) and V(max) with O-phospho-L- homoserine as a substrate are 0.82 mM and 2.42 U/mg. To analyze the role of Phe 97 in the active site of stCGS, we constructed F97Y, R99C, and F97Y-R99C mutant enzymes. Although native stCGS has no activity toward l-methionine, F97Y mutant enzyme gained the elimination activity toward L-methionine. en-copyright= kn-copyright= en-aut-name=ShinozakiMai en-aut-sei=Shinozaki en-aut-mei=Mai kn-aut-name=篠崎舞 kn-aut-sei=篠崎 kn-aut-mei=舞 aut-affil-num=1 ORCID= en-aut-name=YanagitaniMasahiko en-aut-sei=Yanagitani en-aut-mei=Masahiko kn-aut-name=柳谷昌彦 kn-aut-sei=柳谷 kn-aut-mei=昌彦 aut-affil-num=2 ORCID= en-aut-name=KanedaShouichirou en-aut-sei=Kaneda en-aut-mei=Shouichirou kn-aut-name=兼田翔一郎 kn-aut-sei=兼田 kn-aut-mei=翔一郎 aut-affil-num=3 ORCID= en-aut-name=KudouDaizou en-aut-sei=Kudou en-aut-mei=Daizou kn-aut-name=工藤大蔵 kn-aut-sei=工藤 kn-aut-mei=大蔵 aut-affil-num=4 ORCID= en-aut-name=EndouYuuichi en-aut-sei=Endou en-aut-mei=Yuuichi kn-aut-name=遠藤祐一 kn-aut-sei=遠藤 kn-aut-mei=祐一 aut-affil-num=5 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=6 ORCID= en-aut-name=KuramitsuSeiki en-aut-sei=Kuramitsu en-aut-mei=Seiki kn-aut-name=倉光成紀 kn-aut-sei=倉光 kn-aut-mei=成紀 aut-affil-num=7 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=大阪大学大学院理学研究科 affil-num=8 en-affil= kn-affil=岡山大学 en-keyword=cystathionine γ-synthase kn-keyword=cystathionine γ-synthase en-keyword=pyridoxal 5’-phosphate kn-keyword=pyridoxal 5’-phosphate en-keyword=thermoacidophilic archaea kn-keyword=thermoacidophilic archaea en-keyword=Sulfolobus tokodaii kn-keyword=Sulfolobus tokodaii END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=147 end-page=158 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19990226 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Photoconductive and Photovoltaic Properties in Cadmium Bismuth Aluminate Glasses en-subtitle= kn-subtitle= en-abstract= kn-abstract=Photo-induced phenomena such as photoconductive and photovoltaic effects were investigated for the glasses in CdO-Bi(2)O(3)-Al(2)O(3) system. Photoconductive effect was characterized by a slow decay of photocurrent (persitent photoconductivity). The decay rate decreased with increasing CdO content and decreasing Bi(2)O(3) content. Photovoltage was very small at room temperature but increased to an obvious value on heating. The photoconductivity and photovoltage were increased with CdO content and enhanced by heat treatment in air. The valence band spectra of X-ray photoelectron spectroscopy showed that the hybridization of Cd 4d and O 2p orbitals increases with decreasing Bi(2)O(3) content and increasing CdO content in the glasses. As the results maximum tends to flat. This type of band structure inhibits the rapid recombination of electrons and holes. The persistent photoconductivity of the glasses may be attributed to deep energy level of DX centers. Deep energy levels of the glasses are able to prevent the recombination because they have a repulsive barrier for both electron emission and capture. en-copyright= kn-copyright= en-aut-name=ChenDanping en-aut-sei=Chen en-aut-mei=Danping kn-aut-name=陳丹平 kn-aut-sei=陳 kn-aut-mei=丹平 aut-affil-num=1 ORCID= en-aut-name=WatanabeTomohiro en-aut-sei=Watanabe en-aut-mei=Tomohiro kn-aut-name=渡辺智大 kn-aut-sei=渡辺 kn-aut-mei=智大 aut-affil-num=2 ORCID= en-aut-name=MiuraYoshinari en-aut-sei=Miura en-aut-mei=Yoshinari kn-aut-name=三浦嘉也 kn-aut-sei=三浦 kn-aut-mei=嘉也 aut-affil-num=3 ORCID= en-aut-name=NanbaTokuro en-aut-sei=Nanba en-aut-mei=Tokuro kn-aut-name=難波徳郎 kn-aut-sei=難波 kn-aut-mei=徳郎 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Wuhan University of Technology affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Photoconductivity kn-keyword=Photoconductivity en-keyword=Photovoltage kn-keyword=Photovoltage en-keyword=Persistent photoconductivity kn-keyword=Persistent photoconductivity en-keyword=CdO-Bi(2)O(3)-Al(2)O(3) glasses kn-keyword=CdO-Bi(2)O(3)-Al(2)O(3) glasses en-keyword=Electronic state kn-keyword=Electronic state END start-ver=1.4 cd-journal=joma no-vol=31 cd-vols= no-issue=1 article-no= start-page=5 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19961227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Solution-Grown Crystals of Poly(p-phenylene benzobisthiazole) en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rigid polymer poly(p-phenylene benzobisthiazole) was crystallized from dilute solution. Electron microscopy showed that upon quenching, flat fibrils with several nm thick were produced. Subsequent heat treatment in solvent changed the fibril into "shish-kebab". On the other hand, by isothermal crystallization, an aggregate of parallel rod-like crystals was obtained. The molecular chains were accommodated normal to the rod. Based on the observation of crystal morphology, the isothermal crystallization mechanism was proposed. Because of regidity of polymer chains and wide distribution of the molecular length, the chain ends were inevitably included within the crystals resulting in crystal defects such as axial shift, lattice curvature and edge dislocation which were directly observed by lattice imaging. en-copyright= kn-copyright= en-aut-name=ShimamuraKaoru en-aut-sei=Shimamura en-aut-mei=Kaoru kn-aut-name=島村薫 kn-aut-sei=島村 kn-aut-mei=薫 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=ZhangChunxiao kn-aut-sei=Zhang kn-aut-mei=Chunxiao aut-affil-num=2 ORCID= en-aut-name=UchidaTetsuya en-aut-sei=Uchida en-aut-mei=Tetsuya kn-aut-name=内田哲也 kn-aut-sei=内田 kn-aut-mei=哲也 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Applied Chemistry affil-num=2 en-affil= kn-affil=Department of Applied Chemistry affil-num=3 en-affil= kn-affil=Department of Applied Chemistry END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=7 article-no= start-page=2291 end-page=2305 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=19580731 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Serological Study of Cancer in the Field of Oto-Rhino-Laryngology kn-title=耳鼻咽喉科領域に於ける癌の血清学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=By applying three methods, namely, the precipitin reaction in acid medium with the antigen-antibody reaction, the measuring method of heat-noncoagulant protein in determining the serum mucoid content, and the methylene-blue reduction test using the oxdization phenomenon of sulfide ions, on the cancers occurring in the field of oto-rhino-laryngology as the follow-up study, the efficacy of these three methods has been evaluated. The following are the results of the present investigation: In the case of cancer patient before treatment the reliability of the precipitin reaction in acid medium and the measuring method of heat-noncoagulant protein shows the average rate of 80 per cent in general; and these prove to be useful as auxiliary methods for diagnosis. However, the rate of the reliability of the methylene-blue reduction test is poor and therefore its usefulness in diagnosis is low. It is worthy of attention that all these tests gave relatively high rate of positive reaction even in the subjects with diseases such as pharyngeal tuberculosis, peritonsillar abeess and tumors other than cancer. In applying these tests after the treatment, no significant difference can be recognized in the positive rate of methylene-blue reduction test as compared with that before threatment, but in the other two tests the positive rate has been markedly lowered, namely, cancer reaction tended to be negative. With respect to the course after the treatment likewise the methylene-blue reduction test does not coincide with the course of the progress, and only in the case of pharyngeal cancer the recurrence of the cancer can be observed when pseudo-positive or positive continues. The other two methods usually coincide with the course; and in the majority of recurrence cases either positive reaction precedes the recurrence or it turns positive simultaneously with recurrence. Even in the cases with satisfactory improvement, these two methods generally coincide with the progress of the disease after treatment, but there have been a few cases which were positive yet without recurrence. en-copyright= kn-copyright= en-aut-name=DateYoshinori en-aut-sei=Date en-aut-mei=Yoshinori kn-aut-name=伊達良則 kn-aut-sei=伊達 kn-aut-mei=良則 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部耳鼻咽喉科教室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=11-12 article-no= start-page=535 end-page=550 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19721230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Antimitotic factors extracted from marine animals kn-title=数種の海産動物から抽出した細胞分裂調節物質に関する研究 en-subtitle= kn-subtitle= en-abstract=Antimitotic effect were compared among several marine animals, such as, sea cucumber, octopus, mytilus and top shell, according to cornin-extraction method, ammonium sulfate fractionation method and alcholic fractionation method. For the tests of dividing cells were used fertilized sea urchin egg, cultured HeLa cell, CHL cell, HE12TMR cell and explanted Ehrlich ascites cartinoma cell in ddN strain mouse. The experimental results can be summarized as follows. 1) Cornin extracted from sea cucumber complately inhibited on proliferation of cultured cells in 0.1 per cent. And this factor retained in non-dialysable fraction. Sea cucumber cornin, however, did not effect on proliferation of Ehrlich ascites cartimoma cell. 2) Cornin extracted from octopus inhibited on growing of cultured cell in 0.5 per cent. But octopus cornin increased growing of Ehrlich ascites cartinoma cell. 3) Cornin extracted from mytilus inhibited cell division of sea urchin eggs at more than 10(-4)g/ml. And in the case of cultured cell it effected at 0.1 per cent. This factor was found in nondialysable fraction. This factor has no effect on increase Ehrlich cancer cell. Fraction of mytilus got from ammonium sulfate fractionation method showed the effect as same as mytilus-cornin. According to alcholic fractionation method, the fraction of mytilus inhibited growth of Ehrlich cancer cell. We found that some cases recoverd perfectly by intraperitoneally administration of this fraction 20mg a day during 5 days. But this activity was disappeared by heat treatment. 4) Antimitotic factors from top shell-cornin and top shell-alcholic were inhibited on cultured cell at 0.5 per cent. However, did not inhibit on proliferation of Ehrlich cancer cells. 5) Antimitotic factors extracted from these marine animals have maximum absoption at 260mμ, and non-dialysable. kn-abstract=マナマコ，マダコ，ムラサキイガイ，サザエからcornin抽出法，硫安分画法及びアルコール分画法に従って細胞分裂抑制物質の抽出を試みた。分裂細胞としては，ウニの受精卵，in vitroの実験にはHeLa細胞，CHL細胞及び，HE12TMR細胞の培養細胞，in vivoの実験にはEhrlich腹水ガン細胞をddN系マウスに移殖して用いた。1. ナマコから抽出したcorninは培養細胞の増殖を0.1%の濃度で完全に阻害する。そしてこの作用は非透析性分画にある。しかしEhrlich腹水ガン細胞の増殖には効果は少ない。2. タコーcorninは培養細胞に対しては，0.5%の濃度で分裂抑制作用を示すが， Ehrlich腹水ガン細胞の増殖は反って促進させる。3. イガイから抽出したcorninはウニの受精卵の分裂は10-4g/ml以上の濃度で，遅延効果が現れる。培養細胞に対しては，0.1%の濃度で分裂を阻害する。そしてその有効成分は非透析性分画にある。しかしEhrlich腹水ガン細胞の増殖には影響がない。イガイから硫安分画法で得た分画は，イガイのcorninと似た効果を示した。イガイからアルコール分画法で得た分画は，Ehrlichガン細胞の増殖を抑制する。1日20mg連続5日腹腔内注射により，完全治癒の例がみられた。この作用は熱処理することにより失活した。4. サザエから得たcornin分画，アルコール分画共に培養細胞の増殖は，0.5%の濃度で阻害する。しかし，Ehrlich腹水ガン細胞の増殖に対しては対照と差がない。5. 4種の海産動物から抽出した分裂抑制因子は260mμあたりに，吸収の極大を示し，非透析性である。 en-copyright= kn-copyright= en-aut-name=NakaraiAkihide en-aut-sei=Nakarai en-aut-mei=Akihide kn-aut-name=半井昭英 kn-aut-sei=半井 kn-aut-mei=昭英 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 END start-ver=1.4 cd-journal=joma no-vol=83 cd-vols= no-issue=1-2 article-no= start-page=1 end-page=18 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=19710228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Study on a New Simple Method for the Determination of 6 Fractions of Urinary 17-Ketosteroids by Impregnant Thin Layer Chromatography kn-title=尿中17-Ketosteroidの簡易分画測定法に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A new method for simple fractional simultaneous determination of 6 fractions of urinary 17-KS (androstanedione, androsterone, Δ4-androstenedione, etiocholanolone, dehydroepiandrosterone and 11-oxy-17-Ketosteroids) was established and excretion values in normal adult females and several cases with ovarian dysfunction were measured by this method and several fraction ratios were evaluated as necessary informations on their androgenisity. The new method consisted of the following procedures: 170ml of 24 hours urine was adjusted to pH 4.7 with acetic acid and then 6.8ml of 0.2M acetate buffer, 40,800 Fishman units of β-glucuronidase (240 units/ml) and 5,100 units of crystalline penicillin were added, then the urine was incubated in a water bath at 37℃ for 24 hours. Then 20,400 Fishman units of β-glucuronidase were added and it was successively incubated for another 24 hours. The hydrolysed urine was extracted with ethyl acetate three times, 170, 85 and 85ml of ethyl acetate were used. Then, alkali washing with 12.75ml of cold 2N-NaOH twice and water washing with 17ml of distilled water twice were treated to the organic phase, and alkali and water used for washing were reextracted with ethyl acetate. After drying with sodium sulfate anhydrous, the extract was evaporated to residue in vacuo at 60℃. The urine layer after the extraction of β-glucuronidase hydrolysate was saturated with 34g of NaCl, and 17ml of 50% sulfuric acid, 3.4ml of 20% formalin and 170ml of ether were added, and then it was kept standing at room temperature for 4 days being shaken twice a day. After 4 days extraction, another 170ml of ether was used for the reextraction. Each 170ml of ether which extracted solvolysate was washed with 17ml of 1N-NaOH twice and then washed with 21ml of distilled water twice. Alkali and water were reextracted in the same way with the extraction of β-glucuronidase hydrolysate. The layer of ether was dried with sodium sulfate anhydrous and was evaporated to residue at 45℃. Two step hydrolysate was chromatographed as follows; 1: Florisil column chromatography. A glass column 28cm long, of lcm internal diameter was packed with 4g of florisil using chloroform. Steroid residue was absorbed on the column with 3ml×4 of chloroform. The development was carried out with 10ml of chloroform and 80ml of 0.8% v/v methanol-chloroform. The chloroform fraction was discarded as prewash. The fraction of 0.8% methanol-chloroform was evaporated to residue in vacuo at 60℃. The steroid residues obtained thus contained C(19) steroids, and were chromatographed on the following impregnant thin layer chromatography. 2: Impregnant thin layer chromatography. * Preparation of thin layer 20g of Kieselgel GF(254) was mixed vigorously with 40ml of 30% ethyleneglycol/methanol for 3 minutes, and the mixture was spread on a glass plate, 20×20cm, 0.45mm thick. And the plate was allowed to stand at room temperature for few minutes and then it was warmed at 37℃ for 10 minutes. * Spotting and covering Immediately after warming the plate, each steroid residue obtained by column chromatography was spotted on the thin layer and the mixture of 5 authorized pure steroids (11-deoxy-17-KS) was paralelly spotted by 50% methanol-chloroform. After spotting, spotting areas were covered with 50% ethyleneglycol-methanol to keep those areas impregnated. Otherwise, tailing and remained steroid on the spotting area were remarkable. The plate was warmed again at 37℃ for 10 minutes to evaporate methanol. * Development and detection of spots A chromatographic chamber was saturated with organic solvent (10% dichlormethane, 90% n-hexane). The spotted plate was developed in this chamber by the ascending method. When the front of the organic solvent reached 15cm distant from the spotting line, the plate was taken out from the chamber and dried in the air and sulfuric acid was sprayed on the strip of the development of the authorized steroids and then the plate was heated at 110℃ for 20 minutes. Comparing with these spots of standard steroids each residue was separated into the following 6 fractions; androstanedione, androsterone, Δ4-androstenedione, etiocholanolone, dehydroepiandrosterone and 11-oxy-17-KS (from the original spotting area). * Extraction from the impregnant thin layer Each spot was scraped into the centrifuge tube with top and shaked vigorously with 10ml of 50% methanol-chloroform for 5min. Then the tube was centrifuged for 10min. at 3,000 r.p.m. After the organic phase was transfered into the test tube, another 10ml of 50% methanol-chloroform was added to the centrifuge tube and shaked and centrifuged again in the same way. The eluates were collected in the test tube and evaporated at 100℃ in the water bath. Thus 6 fractions of urinary 17-KS were separated easily and recovered satisfactorily. Zimmerman reaction was treated on each sample, simultaneously on 100μg of authorized DHA. After colorimetry at 460-520-580mμ, each fraction was estimated comparing with DHA following correction by Allen's formula. Urinary samples of 5 fractions of 11-deoxy-17-KS were identified comparing with authorized pure steroids by other thin layer chromatographies, gas liquid chromatography, absorption spectra of Zimmerman reaction, Oertel-Eik-Nes reaction, absorption spectra in methanol and in the concentrated sulfuric acid. Excretion values of 6 fractions of urinary 17-KS were determined by this method through a menstrual cycle of 3 adult normal females and through the treatment for induction of ovulation by gonadotropin on 5 patients with ovarian dysfunction. Following determination, fraction ratios were calculated as the rate of each estimated value divided by sum total of urinary 17-KS. It was confirmed that the androsterone ratio and DHA ratio were evaluated as the indicators of androgenicity. By those experiments, the new simple method for fractional determination of urinary 17-KS was established and would be available at every laboratory to get informations of androgenicity such as androsterone ratio and DHA ratio. en-copyright= kn-copyright= en-aut-name=TanakaHideo en-aut-sei=Tanaka en-aut-mei=Hideo kn-aut-name=田中英夫 kn-aut-sei=田中 kn-aut-mei=英夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部産婦人科教室 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=3-4 article-no= start-page=103 end-page=113 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Astudy on permeability of cerebral blood vessels during differential hypothermia kn-title=Differential Hypothermia下における脳血管透過性亢進に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Though many laboratory and clinical trials of tumor heating or cooling have been made for the treatment of intracranial tumors, the effects have been reported to be incomplete and unsatisfactory in most of the cases. In 1965, Popovic et al. reported that differential hypothermia, to keep an experimental tumor normothermic under general body hypothermia, showed more marked effect on the tumor in experimental animals. In the present study, the author investigated the uptake of intravenously administered RISA and sodium fluorescein to the brain tissues of mongrel dogs, in which cold induced lesions were made by a stick of dry ice bilaterally on the cerebral cortices. RISA was administered to those dogs intravenously 3 to 4 hours after making the lesions, while sodium fluorescein 30 to 40 minutes prior to the end of the D.H. treatment, respectively. Under total hypothermia (rectal temperature: 21-23℃), the D.H. treatment was performed for 5 hours. It was found that the uptake of RISA through the cerebral blood vessels was increased in and around the cortical lesions. This change was observed only during the D.H. treatment, and disappeared after the body temperature returned to the normal level. In the observation of sodium fluorescein at the tissue level increased permeabiliity of blood vessels was also observed. en-copyright= kn-copyright= en-aut-name=SugaKen en-aut-sei=Suga en-aut-mei=Ken kn-aut-name=菅健 kn-aut-sei=菅 kn-aut-mei=健 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=5-6 article-no= start-page=527 end-page=536 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=19780630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on anticomplementary activity in systemic lupus erythematosus: Part 2. Experimental studies kn-title=全身性エリテマトーデスの抗補体作用に関する研究 第2編 基礎的考察 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The anticomplementary activity (ACA) of immune complexes or DNA was measured, then those data were compared with that in SLE sera. 1. High ACA was detected mainly in γ-GI and β-Gl fractions of SLE sera. 2. DNA (especially denatured DNA) showed high ACA, and the treatment with DNase eliminated its ACA. DNA did not bind nonspesifically to normal γ-Gl. 3. ACA in some SLE sera was reduced after DNase treatment. This treatment worked mainly on ACA of γ-Gl fraction. 4. The procedure of complement inactivation which is employed in measurement of ACA, depressed ACA of cryoglobulin, experimental immune complexes and SLE γ-GI fraction. This indicated that heat aggregation of native IgG could not account for high ACA in SLE sera. 5. The addition of enough amount of complement reduced ACA of immune complexes, but a certain level of ACA was still retained. 6. ACA of insoluble immune complex was much higher than that of soluble immune complex. These data may suggest that ACA in SLE sera originates from the circulating immune complexes, especially DNA-antiDNA antibody immune complexes and this ACA detected is the reflection of only a part of ACA caused by immune reaction in vivo. en-copyright= kn-copyright= en-aut-name=AmanoTetsuki en-aut-sei=Amano en-aut-mei=Tetsuki kn-aut-name=天野哲基 kn-aut-sei=天野 kn-aut-mei=哲基 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第三内科学教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=7-8 article-no= start-page=955 end-page=977 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Influence of differential hypothermia on transplanted hamster tumor I) Effect of combination with shallow differential hypothermia and antimetabolites kn-title=Differential Hypothermiaのハムスター移植腫瘍におよぼす影響について I) Shallow Differential Hypothermiaと抗癌剤併用の効果について en-subtitle= kn-subtitle= en-abstract= kn-abstract=How to treat the malignant brain tumors has been one of the biggest problems in neurosurgery. It has been in the past, and it is still now. In addition to surgical removal of tumors, various non-surgical method have been tried for the treatment of malignant gliomas. The use of heating or of cooling are some of these methods. They have been tried for a long time. Already in 1866 Busch,W. observed disappearance of sarcoma in patients suffering from erysipelas. Westermark,N. in 1927 observed that rat transplanted tumors were caused to disappear by exposing to heating, while the adjacent normal tissues were not damaged under conditions lethal to the tumors. In 1960 Woodhall,B. heated tumors locally that were perfused with chemotherapy. Shingleton,W.W., in 1962, heated localized tumor tissue on the patients with cancer, combined with regional chemotherapy under generalized hypothermia. According to Popovic,V.P. et'al. in 1965, they were first to report that differential hypothermia, keeping tumors normothermic under total body hypothermia at a temperature of 4℃ during a period of 10 hours in experimental animals, induced disappearance of tumor without resuming their growth afterward. Popovic,V.P., et al. in 1966 carried out further experiments and observed that tumors of the animals disappeared also subsequent to cooling whole body to 4℃ for 1 hours with anti-tumor agent, as well as to cooling whole body to 30℃ for 24 hours without chemotherapy while the tumor kept at 37℃. However, in order to induce tumor disappearance the differential hypothermia has to last at least 4℃ for 1-10 hours, or 30℃ for 24 hours. Since deep hypothermia lasting several hours or shallow hypothermia for long time is not well tolerated in non-hibernators, namely human beings, present experiment has been performed in an attempt to simulate the conditions of clinical work as close as possible. For this purpose the bodies of 22 hamsters were mildly cooled to a temperature of 30℃, while cheek pouch transplanted tumors induced originally by adenovirus type 12 remained uncooled at 37℃ for 10 hours (Fig. 1, 2). The dose of 50mg/kg of 5-fluorouracil (5-FU) was administered intraperitoneally in single injection at the beginning of treatment of shallow differential hypothermia (Table 1, Fig. 2). This resulted in that within 7 days later 4 out of 22 tumors (20 % ) disappeared completely without resuming their growth afterward, and 12 out of 22 tumors (55 % ) regressed temporarily for a period of 10 days after treatment (Table 2, 3, Fig. 6-b, 8). When the same amount of 5-FU was administered into normothermic tumor-bearing animals or into hypothermic animals with hypothermic tumors, tumor size of the animals was not affected (Table 1, Fig. 5-a, 6-a). When shallow differential hypothermia was treated without any anti-tumor agent, neither normothermic tumor size nor hypothermic one of the animals was also affected (Table 1, Fig. 7-a). Histological findings were degeneration of tumor cells. Stainability of nucleolar RNA and nuclear DNA by means of methyl green-pyronine and Feulgen's methods was decreased already immediately after the shallow differential hypothermia treatment for 10 hours with 5-FU administration (Fig. 10-a). This was followed by, at 24 hours after the treatment, marked decreasing and loss of the stainability of nucleolar RNA and nuclear DNA as well as dismixture of chromatin and karyolysis of nuclei (Fig. 11-a, b). Scattered necrotic foci in the tumor tissue were obviously revealed 48 hours after the treatment. Tumor cells showed selectively these degenerative finding after treatment, while adjacent mucosal tissue of cheek pouch preserved their normal appearance almost wholly even 12 hours after the treatment (Fig. 10-c). Although inflammatory cell infiltration was observed 48 hours after the treatment, and followed by fibrocytic fibrosis in the submucosa thereafter, degenerative and necrotic change was never showed (Fig. 11-c, 12-a, b). The author discussed that 1) combination with differential hypothermia and anti-tumor agent was effective for experimental tumor, 2) selective destroying and normothermic state of the treatment should be useful for non-surgical therapy of malignant brain tumor, as well as 3) cellular sensitive function of differential hypothermia might be exsisted in nucleolus or nucleus of tumor cell. en-copyright= kn-copyright= en-aut-name=OhashiTakeo en-aut-sei=Ohashi en-aut-mei=Takeo kn-aut-name=大橋威雄 kn-aut-sei=大橋 kn-aut-mei=威雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=1-2 article-no= start-page=33 end-page=46 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=19780228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Influence of differential hypothermia on Vx2 tumor transplanted in rabbit Part 1. Effect of differential hypothermia combined with or without anti-tumor agent kn-title=家兎Vx2移植腫瘍におよぼすDifferential Hypothermiaの影響 第1編 Differential Hypothermia単独および抗癌剤併用時の効果について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Many attempts to inhibit the growth of tumors by heating or cooling have been made, but the results have been reported to be incomplete and unsatisfactory in most of the cases. However, in 1965, Popovic and his coworkers reported that the differential hypothermia (D.H.), to keep the tumor normothermic and the total body hypothermic, showed a more marked effect on the tumor in experimental animals. In the present study, the author investigated the effects of D.H. treatment on Vx2 transplantable tumor in rabbit. The tumor was subcutaneously transplanted into the bilateral ears and, 7 days after, the unilateral tumor was treated by 10-hour D.H. treatment under the condition of the total body temperature at 23~24℃ and the tumor at 37℃. This experiment was compared with or without combination with anti-tumor agent (5-fluorouracil 10mg/kg i.v.). It was found the growth of Vx2 tumor was inhibited when D.H. treatment and 5-fluorouracil (5-FU) administration were performed. The tumors treated by D.H. treatment combined with 5 FU administration disappeared in 3 cases and regressed in 5 cases out of 10, showing the most favourable results than other treatments. Histological examination of the treated tumors revealed the degenerative process in the nuclei of the treated tumor cells followed by pyknoses and karyorrhexes. en-copyright= kn-copyright= en-aut-name=YamadaOsamu en-aut-sei=Yamada en-aut-mei=Osamu kn-aut-name=山田修 kn-aut-sei=山田 kn-aut-mei=修 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=7-8 article-no= start-page=883 end-page=894 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Co-existence of inhibitory and stimulatory factors on cell proliferation in a same rat liver supernatant and their regenerating changes kn-title=ラット肝上清中に共存せる細胞増殖抑制因子と促進因子の肝再生誘導による活性変換について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Responding mechanism of regenerating hepatocytes induced by partial hepatectomy was studied. 1) Fibroblast inhibitory and stimulatory factors on fibroblast proliferation co-exist in the same rat liver supernatant, and they were simply isolated each other by ethanol fractionation. 2) These factors have protein components and lose the biological activities by heat-treatment, and they show the competitive activity each other, respectively. 3) In regenerating liver, stimulatory factor(s) was not remarkably changed, but inhibitory one almost lost the inhibitory activity. These phenomena are very rational and suitable for hepatocyte regeneration. 4) The inhibitory activity was separatedly eluted by DEAE-cellulose chromatography of inactive regenerating liver fraction, and new peak that did not exist in normal inhibitory fraction was detected in polyacrylamid gel electrophoresis of regenerating liver same fraction. 5) These phenomena suggest that a possible new anti-inhibitory factor(s) would be formed by partial hepatectomy. en-copyright= kn-copyright= en-aut-name=FujiiToshitake en-aut-sei=Fujii en-aut-mei=Toshitake kn-aut-name=藤井利武 kn-aut-sei=藤井 kn-aut-mei=利武 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=9-10 article-no= start-page=1007 end-page=1014 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19801030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on protein -thermosoluble protein- and its Donaggio activity in urine after physical exercise kn-title=生体負担時の尿中蛋白（特に熱可溶性蛋白）及びそのDonaggio反応について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Acid and heat-resistant proteins (thermosoluble proteins) were isolated from urine after exercise. These proteins were divided by starch block electrophoresis into 5 subfractions with 30 sections. Donaggio titer and protein measurement were done for each section, and the relationship between thermosoluble proteins and Donaggio specific activity (Donaggio titer/total protein concentration) was investigated. The results were: 1) The Donaggio specific activity of the 5 subfractions with 30 sections was maximum in the alb. ~α1-glob. region. It increased after treatment by heating at 100℃ for 10 minutes and acidification to pH 5. 2) A significant rise in the protein concentration of urine after exercise was observed in the alb. and αl-glob. regions. 3) The Donaggio specific activity of serum was higher than urine for equivalent protein concentrations. 4) Thermosoluble proteins were identified as Donaggio substances. The Donaggio specific activity of proteins was in the order of alb.,>α1-glob.>α2-glob.≫β-glob., γ-glob.. 5) The most important proteins contributing to the Donaggio titer were thought to be alb. and αl-AG, judging from correlation coefficients between Donaggio titers and protein measurement. The differences between alb. and αl-AG, before and after treatment were not statistically significant. Measurement of thermosoluble proteins in urine may be a useful method of determining the magnitude of the load imposed by physical exercise upon an individual. en-copyright= kn-copyright= en-aut-name=OhmoriSachio en-aut-sei=Ohmori en-aut-mei=Sachio kn-aut-name=大森祥夫 kn-aut-sei=大森 kn-aut-mei=祥夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=Donaggio 反応 kn-keyword=Donaggio 反応 en-keyword=Donaggio 特異活性 kn-keyword=Donaggio 特異活性 en-keyword=熱可溶性蛋白 kn-keyword=熱可溶性蛋白 END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=3-4 article-no= start-page=367 end-page=385 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19800430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The effects of heat on the growth and uptake of anti-cancer agent of tumors implanted in hamster cheek pouch kn-title=ハムスター頬嚢移植腫瘍の増殖並びに抗癌剤取り込みに及ぼす温熱の効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract=SV40 induced tumor cells were inoculated to hamster cheek pouches. When the tumor was about 6 mm in diameter, tritiated-5-fluorouracil ((3)H-5-FU), 1 μCi/g body weight, was administered intraperitoneally, and the unilateral tumor of each animal was warmed at 37℃ or 40℃ under total body hypothermia (25℃). The uptake of (3)H-5-FU into the tumors kept at 37℃ for 60 minutes increased about 70% as compared with that of the contralateral hypothermic tumor, whereas 80% increase of the uptake was observed with the tumors kept at 40℃ for 60 minutes. The uptake of (3)H-5-FU into the tumors of non-treated normothermic animals was higher than that of normothermic tumors under total body hypothermia. Autoradiography revealed that silver grains were diffusely present in the tumor tissue. Their density seemed to increase in parallel with the radioactivity of (3)H-5-FU measured with a scintillation counter. The results suggested that capillary permeability might be increased in normothermic tumors under total body hypothermia, whereas circulatory disturbances might develop in the hyperthermic tumors. In order to estimate the anti-tumor effects of the thermal treatment combined with anti-cancer agents, 5-fluorouracil (5-FU), 50 mg/kg, was administered intraperitoneally, and the unilaterally implanted SV40 induced tumors (about 6 mm in diameter) of hamster cheek pouch were kept at 37℃ or 40℃ under total body hypothermia (25℃). Thirty days later, the growth of the tumors kept at 37℃ for 10 hours combined with 5-FU administration was markedly inhibited as compared with that of non-treated control tumors. As for the tumors kept at 40℃ for 8 hours, 20% of the tumors disappeared without 5-FU administration, and 80% of the tumors disappeared with 5-FU administration. It was concluded that hyperthermia (40℃) on the tumor under total body hypothermia had a greater anti-cancer effect than normothermia (37℃). en-copyright= kn-copyright= en-aut-name=MiyakeIkuo en-aut-sei=Miyake en-aut-mei=Ikuo kn-aut-name=三宅幾男 kn-aut-sei=三宅 kn-aut-mei=幾男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科学教室 en-keyword=Experimental tumor kn-keyword=Experimental tumor en-keyword=thermotherapy kn-keyword=thermotherapy en-keyword=differential hypothermia kn-keyword=differential hypothermia en-keyword=anti-cancer drug kn-keyword=anti-cancer drug END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=9-10 article-no= start-page=1317 end-page=1332 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental study of safety limits in differential hypothermia Part 2. Metabolic effects on normal rabbit brains kn-title=Differential Hypothermia処置の安全限界に関する実験的研究 第2編 家兎正常脳の代謝への影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Differential hypothermia (D.H.) treatment was performed on normal rabbit brains and the associated changes in cerebral metabolism were observed in series. Under generalized body hypothermia (rectal temperature; 23℃), a portion of normal brain was maintained at normothermia (37±1℃) by heating with microwave irradiation (2.45GHz). Brain tissues were sampled as follows; before treatment, at the beginning, 3, 6 and 10 hours later, and finally 12 and 36 hours after completion of the 10 hour-treatment. Five rabbits were studied in each group to be 35 as a total sum. In each rabbit, two samples; one from the normothermic portion and one from the hypothermic portion, were obtained. By using enzymatic analysis, six glycolytic metabolites (glucose, glucose-6-phosphate, fructose-1,6-diphosphate, dihydroxyacetone phosphate, pyruvate, lactate) and three adenine nucleotides (ATP, ADP, AMP) were investigated quantitatively. From these results, the ratio of lactate and pyruvate (L/P ratio) and energy charge potential (ECP) were calculated. Before the treatment, the L/P ratio was 26.4 and ECP was 0.921. Up to 6 hours after the beginning, the metabolic status in the normothermic portion was relatively higher than the hypothermic portion. Namely, L/P ratio and ECP in the normothermic portion reached 27.6 and 0.953 respectively. On the completion of the 10 hour-treatment, however, glucose decreased considerably. While G6P increased markedly associated with a L/P ratio of 47.5. Similarly ATP was decreased, while ADP and AMP were increased, therefore ECP decreased to 0.873. These data indicated tissue hypoxia. On the other hand, 36 hours after the treatment, the L/P ratio recovered completely to 18.7, ECP also recovered to 0.901. These results indicated that metabolism in the normothermic portion was smooth up to 6 hours duration of treatrent, although it was constantly higher than the hypothermic portion. On the completion of the 10 hour-treatment, increase of glycolysis by tissue hypoxia and failure of energy metabolism were observed. However, these changes were considered still reversible because the results obtained after the treatment indicated reasonable recovery. Therefore it was suggested that 10 hour-differential hypothermia treatment on the normal rabbit brains was safe from a viewpoint of glycolytic and energy metabolism. en-copyright= kn-copyright= en-aut-name=IkedaKomei en-aut-sei=Ikeda en-aut-mei=Komei kn-aut-name=池田幸明 kn-aut-sei=池田 kn-aut-mei=幸明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科学教室 en-keyword=differential hypothermia kn-keyword=differential hypothermia en-keyword=normal brain kn-keyword=normal brain en-keyword=glucose kn-keyword=glucose en-keyword=adenine nucleotides kn-keyword=adenine nucleotides en-keyword=metabolic changes kn-keyword=metabolic changes END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=9-10 article-no= start-page=1301 end-page=1316 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental study of safety limits in differential hypothermia Part 1. Heating of brain by microwave irradiation kn-title=Differential Hypothermia処置の安全限界に関する実験的研究 第1編Microwave照射による脳加温に関して en-subtitle= kn-subtitle= en-abstract= kn-abstract=Differential hypothermia (D.H.) treatment (preservation of brain tumor at normothermia during generalized body hypothermia) for the treatment of human brain tumors requires the tumor to be heated throughly and maintained for the necessary time. The normal portion of brain has to be protected during and after the treatment. Microwave (MW) seemed the most suitable heating method, so its effects on the normal brain of dogs were studied. During MW irradiation, the thermistor-probe showed higher temperatures erroneously. When the probe was covered by aluminium foil, the measurement error was less than 0.2℃ as long as the probe was in the brain. This method sufficiently heated the brain under generalized hypothermia down to 2 cm in depth from the surface. The highest temperature was recorded between 0.5 cm and 1 cm in depth and was easily controlled by the output of MW. On the other hand, aluminium foil blocked MW irradiation completely, therefore the irradiation field possibly protected the normal portion. To study the safety limits of heating by MW irradiation, two groups of rabbits maintained at different temperatures (37℃ and 40℃) were prepared. Following D.H. treatment for 5 hours in each group, the rabbit brains were examined histologically. The results showed minimal brain damage by MW irradiation in the 37℃ group. In the 40℃ group, heat necrosis was constantly observed as well as stagnation of vessels and perivascular cuffing of round cells. From these findings, it was concluded that MW was useful as a heating method during D.H. treatment. The safety limit of heating was up to normothermia. en-copyright= kn-copyright= en-aut-name=IkedaKomei en-aut-sei=Ikeda en-aut-mei=Komei kn-aut-name=池田幸明 kn-aut-sei=池田 kn-aut-mei=幸明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科学教室 en-keyword=differential hypothermia kn-keyword=differential hypothermia en-keyword=microwave irradiation kn-keyword=microwave irradiation en-keyword=normal brain kn-keyword=normal brain en-keyword=histopathology kn-keyword=histopathology END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=1-2 article-no= start-page=153 end-page=164 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19800228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Inhibitors of cell proliferation in a rat liver supernatant kn-title=ラット肝上清中に存在する細胞増殖抑制因子について en-subtitle= kn-subtitle= en-abstract= kn-abstract=The activities and properties of two inhibitors of cell proliferation, which are present in the supernatant of rat liver, have been studied in L(929) fibloblast systems. 1) One factor, which had arginase activity, completely inhibited cell proliferation by depletion of arginine in the culture medium. 2) The other factor was stable to Trypsin-, RNase and Heat-treatment, and primarily inhibited protein synthesis. Inhibition of DNA synthesis was thought to be a secondary effect. 3) Whether the second factor changes the biological activity in regenerating liver is now under investigation. en-copyright= kn-copyright= en-aut-name=KuramitsuMakoto en-aut-sei=Kuramitsu en-aut-mei=Makoto kn-aut-name=倉光誠 kn-aut-sei=倉光 kn-aut-mei=誠 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 en-keyword=細胞増殖抑制因子 kn-keyword=細胞増殖抑制因子 en-keyword=ラット肝上清 kn-keyword=ラット肝上清 en-keyword=L細胞 kn-keyword=L細胞 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=261 end-page=279 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Biological effects of 2,450 MHz microwave radiation on rat glioma cells in vitro kn-title=培養ラットグリオーマ細胞(C6)に対するマイクロウエーブ照射の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A monolayer culture of a clonal cell strain of rat glioma was exposed to 2,450 MHz microwave (continuous wave) radiation. The clonal cell strain was the C6 strain established by Benda, et al. (22). The temperature of the medium was measured with a thermistor probe placed under each culture bottle, and it was maintained at 37℃ or at 38.5℃ during 10-hour irradiation, and similarly at 40℃ during 5-hour irradiation. After each irradiation, the culture bottle was incubated at 37℃ for 48 hours. The cell growth of C6 cells exposed to microwave radiation for 10 hours did not show any changes as long as the temperature of the medium was kept lower than 38.5℃. However, when the temperature was kept at 40℃ for 5 hours by microwave irradiation, C6 cells significantly decreased (63%) in numbers on the 2nd day after the treatment (Table 1). Ten days later, these cells resumed their exponential growth and their population doubling time was same as that of nontreated C6 cells (Fig. 9). On the other hand, the treatment of the cells at 40℃ for 5 hours in a conventional incubator resulted in only a 20% decrease in cell numbers and 10-hour treatment at 41.5℃ resulted in a 48 % decrease in the number of C6 cells (Fig. 9). The difference between the effects on cell numbers of microwave radiation and conventional heat incubation suggest that the temperature distribution within the culture bottle might not be uniform under microwave irradiation. Therefore growth inhibition of the cells after hyperthermic (40℃) microwave irradiation was considered probably due to the thermal effects rather than to the nonthermal effects of electromagnetic irradiation. After microwave irradiation, May-Giemsa staining of surviving cells did not reveal any morphological abnormalities (Fig. 6). Feulgen reaction of the cells did not reveal any changes in the number of nuclear DNA particles, either (Fig. 7). In addition, chromosome analysis, performed on the 2nd and the 10th day after microwave radiation, did not show any appreciable chromosome damage. One hundred metaphases of C6 cells were examined for each analysis and 84% of them had the stemline number of 40 chromosomes (Table 2). The karyotype of these cells was not different from that of nontreated C6 cells. In the stemline karyotype, five chromosomes were missing: one chromosome of the A2 pair, one chromosome of the A3 pair, one chromosome of the C12 pair, and two chromosomes from group B. There were also constant gains of three markers (Fig. 8). The present results indicate that 2,450 MHz continuous wave radiation on the C6 cells did not reveal any nonthermal effects of electromagnetic radiation, in contrast to several other reports: Webb and Dodds (47), Yao, et al. (48), Okai (50), Webb and Booth (59), and Heller (67). en-copyright= kn-copyright= en-aut-name=HaradaYasuhiro en-aut-sei=Harada en-aut-mei=Yasuhiro kn-aut-name=原田泰弘 kn-aut-sei=原田 kn-aut-mei=泰弘 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科学教室 en-keyword=マイクロウエーブ kn-keyword=マイクロウエーブ en-keyword=生物学的作用 kn-keyword=生物学的作用 en-keyword=C6ラットグリオーマ細胞 kn-keyword=C6ラットグリオーマ細胞 en-keyword=組織培養 kn-keyword=組織培養 END start-ver=1.4 cd-journal=joma no-vol=93 cd-vols= no-issue=1-2 article-no= start-page=25 end-page=30 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Donaggio reaction positive substances derived from serum protein (Cohn fraction VI) kn-title=血清分画(Cohn fraction VI)から得られるDonaggio反応陽性物質に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Up to date Donaggio reaction positive substances derived from serum protein have been shown to be from the α(1)-globulin region, mainly α(1)-AG. One purpose of this study was to purify α(1)-AG from Cohn fraction VI of human serum by means of starch block electrophoresis and to measure the Donaggio titer in it. The other purpose was to compare α(1)-AG with the other protein subfraction. The results were: 1) Pure (about 98%) α(1)-AG was obtained from Cohn fraction VI after heating. The Donaggio titer of α(1)-AG solution was 1.7-2.2/mg/dl. Whole Cohn fraction VI was 1.2, human albumin was 0.7 and α(2)-HS was 0.8. 2) The Donaggio specific activity was indirectly proved to be in the order of α(1)-globulin>α(2)-globulin contained in the Cohn fraction VI. This result supports the previous report. 3) A solution of Cohn fraction VI was almost equal in Donaggio titer per the same concentration of protein to urine after exercise, after treatment by heating at 100℃ for 10 minutes and acidification to pH. 5 by acetic acid. 4) α(1)-AG was concluded to have the highest Donaggio titer of the whole protein subfraction. en-copyright= kn-copyright= en-aut-name=OhmoriSachio en-aut-sei=Ohmori en-aut-mei=Sachio kn-aut-name=大森祥夫 kn-aut-sei=大森 kn-aut-mei=祥夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=Donaggio反応 kn-keyword=Donaggio反応 en-keyword=Cohn fraction VI kn-keyword=Cohn fraction VI en-keyword=α(1)-acid glycoprotein kn-keyword=α(1)-acid glycoprotein END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=7-8 article-no= start-page=665 end-page=674 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=19840830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Establishment of a T cell hybridoma continuously producing Interleukin 2 (IL2) and functional analysis of secreted IL2 kn-title=IL2産生性T細胞ハイブリドーマの樹立とその免疫学的諸性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The AKR-derived T lymphoma cell line BW5147 was fused with a Mycobacterium tuberculosis-primed and boosted BALB/c T cell to produce a T cell hybridoma (A55-24) which continuously produces Interleukin 2 (IL2). A55-24 cells produce IL2 without stimulation by lectin or antigen. The hybridoma culture supernatant did not contain other lymphokine-activities, T cell-replacing factor (TRF), B cell growth factor (BCGF), or macrophage activating factor (MAF). The secreted IL2 possessed the following characteristics: 1) IL2 activity was precipitated at an ammonium sulphate saturation of 50-85% . 2) a molecular weight of 30 Kd 3) an isoelectric point of pH 5.3±0.2 4) IL2 activity was destroyed only partially by pH 2 treatment and heating (56℃, 30min), but eliminated by boiling (100℃, 10min). 5) IL2 activity was absorbed by an IL2-dependent cytotoxic T cell line. 6) Addition of IL1 and this IL2 could not generate any significant CTL responses from PNA(+)-thymocytes, whereas further addition of PPD-CFS obtained from PPD-stimulated Mycobacterium-primed T cells induced noticiable responses. en-copyright= kn-copyright= en-aut-name=KanataniTomohisa en-aut-sei=Kanatani en-aut-mei=Tomohisa kn-aut-name=金谷誠久 kn-aut-sei=金谷 kn-aut-mei=誠久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部細菌学教室 en-keyword=interleukin 2 kn-keyword=interleukin 2 en-keyword=T cell hybridoma kn-keyword=T cell hybridoma en-keyword=lymphokine kn-keyword=lymphokine en-keyword=cytotoxic T lymphocyte kn-keyword=cytotoxic T lymphocyte END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=3-4 article-no= start-page=387 end-page=393 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19830430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Partial purification and properties of Ca(2+)-binding protein from rat liver mitochondrial matrix. kn-title=ラット肝ミトコンドリア・マトリクスのCa(2+)結合蛋白質の部分精製とその物性について en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 193-fold purification of Ca(2+)-binding protein from rat liver mitochondrial matrix was achieved. The Ca(2+)-binding protein consisted of 3 polypeptide subunits whose respective molecular weights by SDS polyacrylamide gel electrophoresis were 62K, 49K and 37K. The molecular weight of the protein was 150K to 220K. The Kd for Ca(2+) was 1.3×10-(5)M and lower for Mn(2+) and Mg(2+). The protein was inactivated by heat treatment at 100℃ for 1 min, though stable against treatment with 0.5% w/v trypsin at 37℃ for 30 min. Ruthenium red did not inhibit Ca(2+)-binding. en-copyright= kn-copyright= en-aut-name=TokudaMasaaki en-aut-sei=Tokuda en-aut-mei=Masaaki kn-aut-name=徳田雅明 kn-aut-sei=徳田 kn-aut-mei=雅明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 en-keyword=肝ミトコンドリア kn-keyword=肝ミトコンドリア en-keyword=Ca(2+)結合蛋白質 kn-keyword=Ca(2+)結合蛋白質 END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=1-2 article-no= start-page=149 end-page=160 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=19840228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental study on pathogenesis and treatment of cerebral vasospasm. Part 1. Effects of thromboxane A(2) synthetase inhibitor (OKY-046) on experimental cerebral vasospasm. kn-title=脳血管攣縮の発生機序と治療に関する実験的研究 第1編 実験的脳血管攣縮に対するThromboxane A(2)(TxA(2))合成酵素阻害剤(OKY-046)の効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of a thromboxane A(2) synthetase inhibitor (OKY-046: sodium (E)-3-[4-(1-imidazolylmethyl)]-2-propenoate) on the diameter of normal and constricted basilar arteries and on the regional cerebral blood flow (r-CBF) in the brain stem after experimental subarachnoid hemorrhage (SAH) were investigated in 27cats. The basilar artery was exposed transclivally, and the change in its diameter was studied by serial photographs. Both normal and constricted basilar arteries showed only slight dilatation after an intravenous injection of OKY-046 (30mg/kg, 60mg/kg). Mean arterial blood pressure (MABP) decreased dose dependently with the administration of OKY-046. Exprimental SAH was produced by an intracisternal injection of 3ml of fresh autologous arterial blood. Three days later, the basilar artery was exposed transclivally, and an advanced prolonged vasospasm was produced by topical application of a blood-CSF mixture incubatesd at 37℃ for 3days. The change in r-CBF in the brain stem was measured continuously by the heat clearance method before, during and after intravenous administration of OKY-046 (30mg/kg, 60mg/kg). The changes of r-CBF in cats with experimental SAH (n=9) were divided into three types: no change in r-CBF (n=3), a transient decrease in r-CBF related to the decrease in MABP (n=1) and an increase in r-CBF in spite of systemic lypotension (n=5). In 3 control cats, r-CBF decreased in relation to systemic hypotension. These results indicate that thromboxane A(2) is not the major factor of cerebral vasospasm. However, OKY-046 which has been known to inhibit platelet aggregation and to prevent vascular constriction, might be useful in the prophylaxis and treatment of ischemic symptoms in patients with cerebral vasospasm. en-copyright= kn-copyright= en-aut-name=MotokiMototsugu en-aut-sei=Motoki en-aut-mei=Mototsugu kn-aut-name=元木基嗣 kn-aut-sei=元木 kn-aut-mei=基嗣 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科教室 en-keyword=cerebral vasaspasm kn-keyword=cerebral vasaspasm en-keyword=prostaglandin kn-keyword=prostaglandin en-keyword=prostacyclin kn-keyword=prostacyclin en-keyword=thromboxane A(2) kn-keyword=thromboxane A(2) en-keyword=OKY-046 kn-keyword=OKY-046 END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=11-12 article-no= start-page=927 end-page=936 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19851230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Properties of the catalase molecule obtained from acatalasemic and hypocatalasemic mice Part I. Effects of denaturants on the catalase activity in the mouse liver kn-title=無および低カタラーゼ血症マウスのカタラーゼ分子の性質に関する研究 第1編 マウス肝カタラーゼ活性に対する変性剤の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Homogenates of mouse liver with isotonic sucrose solution were separated by the cell fractionation with repeating centrifugation. The supernatants were used for the inhibition test with the reagents such as 3,5 diiodosalicylic acid lithium salt (LIS), guanidine and azide, heat, acid and alkali. After various treatments, the remaining catalase activities were measured and showed as a relative enzyme activity. Stability of catalase in liver supernatants was compared normal (C3H/C(as)C(as)) and mutant mice which were designated acatalasemia (C3H/C(bs)C(bs)), hypocatalasemia (C3H/C(cs)C(cs)) and acatalasemic heterozygote (C3H/C(as)C(bs)). In both treatments of LIS and guanidine, catalase of C(as)C(as) was most stable, C(bs)C(bs) were unstable, and catalase of C(cs)C(cs) and C(as)C(bs) showed midle stability between the stability of C(as)C(as) and C(bs)C(bs) in this order. On the contrary in azide treatment within the range from O to 1mM azide, C(bs)C(bs) was most stable, and C(as)C(as), C(cs)C(cs) and C(as)C(bs) were unstable in this order, but more than 1mM azide, C(cs)C(cs), C(as)C(as) and C(as)C(bs) were unstable in this order. After incubation at various temperatures changing from 30℃ to 60℃ for 10min, C(as)C(as) was most stable, and C(as)C(bs), C(cs)C(cs) and C(bs)C(bs) were unstable in this order. After acid and alkali treatments in the range of pH 5.5 to 9.0, relative activities of C(cs)C(cs) and C(as)C(bs) were similar to that of C(as)C(as), but C(bs)C(bs) was less stable than C(as)C(as), C(cs)C(cs) and C(as)C(bs) in the same range. It is considered that the structure of catalase molecule in the liver is different between normal, mutant mice and heterozigote of normal and mutant mice with regard to the difference of the stability to LIS, guanidine, azide, heat, acid and alkali treatments. en-copyright= kn-copyright= en-aut-name=SatoYukinori en-aut-sei=Sato en-aut-mei=Yukinori kn-aut-name=佐藤征紀 kn-aut-sei=佐藤 kn-aut-mei=征紀 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=グアニジン kn-keyword=グアニジン en-keyword=LIS kn-keyword=LIS en-keyword=アジド kn-keyword=アジド en-keyword=温度 kn-keyword=温度 en-keyword=pH kn-keyword=pH END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=5-6 article-no= start-page=655 end-page=667 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of heat on regional cerebral blood flow of the normal monkey kn-title=温熱の正常サル局所脳血流量に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effect of the tissue temperature on regional cerebral blood flow (rCBF) was studied using anesthetized and respiratory-controlled normal monkeys. rCBF was sequentially measured by the inhalation hydrogen clearance method, during localized brain heating under generalized hypothermia or differential hypothermia (DH), and was compared to that obtained by whole-body heating and cooling (WB). For WB in 6 animals, the body temperature was controlled by immersion of the body in a hot or cold water bath. rCBF changed with a change in the tissue temperature in the range of 27.8 to 39.7℃,showing a constant 12.2% per degree Celsius change. For DH in 7 animals, the brain was locally heated by external 2450 MHz microwave irradiation, while the body was kept hypothermic (30.0-30.8℃) by immersion in a cold water bath. rCBF rate of 15.2% per degree Celsius change was obtained in response to change in the tissue temperature ranging from 29.4 to 40.7℃. The results indicated that rCBF changed proportionately with the change in the tissue temperature within a specific temperature range between shallow hypothermia and modest hyperthermia.rCBF changed at a higher rate after DH treatment than was possible by WB treatment. The response of the rCBF to changes in the tissue temperature of the normal brain may play a significant role in thermotherapy for brain tumors. en-copyright= kn-copyright= en-aut-name=SatohToru en-aut-sei=Satoh en-aut-mei=Toru kn-aut-name=佐藤透 kn-aut-sei=佐藤 kn-aut-mei=透 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科学教室 en-keyword=brain tumor kn-keyword=brain tumor en-keyword=cerebral blood flow kn-keyword=cerebral blood flow en-keyword=differential hypothermia hyperthermia kn-keyword=differential hypothermia hyperthermia en-keyword=hypothermia kn-keyword=hypothermia END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=5-6 article-no= start-page=431 end-page=442 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=19870630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Fundamental study of differential hypothermia treatment of malignant brain tumors -Evaluation of 915 MHz microwave irradiation for therapeutic heating of the brain- kn-title=悪性脳腫瘍に対する区別低体温療法(Differential hypothermia)の基礎的研究 ―915MHzマイクロウェーブ照射による脳加温効果の検討― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Differential hypothermia (D.H.), in which tumors are kept normothermic under total body hypothermia, has been confirmed to have a therapeutic effect. It is essential in the treatment of malignant brain tumors by D.H. to deliver the heat uniformly to specific volumes of tissue and to maintain the optimal temperature for an appropriate period of time. A new instrument for delivering electromagnetic radiation at 915 MHz was devised, and its therapeutic efficacy was evaluated in comparison with conventional 2,450 MHz microwave diathermy. Adult canine or monkey brains were exposed to microwave irradiation following fronto-parietal craniectomy under total body hypothermia (29±1℃). The highest temperture in the brain was controlled manually to be between 3-37℃. The average temperature at depths of 0, 0.5, 1.0, 1.5, 2.0 and 3.0 cm was 34.8℃, 35.5℃, 36.0℃, 36.7℃, 34.4℃, and 30.7℃, respectively, at 915 MHz ang 3.8℃, 35.4℃, 36.0℃, 3.℃, 34.5℃, and 32.0℃, respectively, at 2,450 MHz. Deeper heat penetration was achieved with 915 MHz than with 2,450 MHz MW irradiation. The temperature of the brain was controlled easily and could be kept 7-10℃ higher than that of the cooled body. Heat toxicity was examined histopathologically following a 5-hour-D.H. treatment. No brain damage was observed in brains heated to 37℃, but irreversible changes such as exudative homorrhage were revealed in brains kept at 40℃. These results suggest that : 1) 815 MHz MW irradiation can effectively induce localized brain hyperthermia. 2) Deeper heat penetration was achieved by 915 MHz than 2,450 MH MW irradiation. 3) Temperatures above 40℃ for 5 hours induced irreversible changes in the brain under total body hypothermia (29±1℃). en-copyright= kn-copyright= en-aut-name=MatsumotoKengo en-aut-sei=Matsumoto en-aut-mei=Kengo kn-aut-name=松本健五 kn-aut-sei=松本 kn-aut-mei=健五 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科教室 en-keyword=Malignant brain tumor kn-keyword=Malignant brain tumor en-keyword=Differential hypothermia kn-keyword=Differential hypothermia en-keyword=Microwave kn-keyword=Microwave END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=1-2 article-no= start-page=155 end-page=167 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of Ca antagonists on experimental cerebral vasospasm kn-title=脳血管攣縮に対するCa拮抗剤の効果 ―実験的研究― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cerebral vasospasm is the most severe complication of subarachnoid hemorrhage. Although the etiology of vasospasm is still obscure, it has been suggested that some Ca antagonists can relieve cerebral vasospasm. The effects of Ca antagonists (Nifedipine, Nicardipine, Verapamil, Cinnarizine, Diltiazem) on the diameter of the basilar artery and rCBF in the brain stem were investigated in cats with experimental cerebral vasospasm. Three days after an intracisternal injection of blood (3ml), the basilar artery was exposed by the transclival approach. Experimental cerebral vasospasm was induced by the topical application of a blood CSF mixture. The rCBF was measured by the heat clearance method, and the diameter of the basilar artery was examined in serial photographs. Whenever Ca antagonists were given, the mean arterial blood pressure (MABP) decreased dose dependently. Ca antagonists can dilate (from 7.1% to 45% 10 minutes after the administration of Ca antagonists) the normal basilar artery, but can hardly change the basilar artery of cats with experimental cerebral vasospasm. The response of the rCBF in the brain stem after the administration of Ca antagonists was of 3 types: continuous increase, transient increase and no response. The greater the MABP decreased, the higher the possibility of continuous increase and transient increase among all cases was. The possibility of continuous increase and that of transient increase were higher in the control group than that in the vasospasm group. However, even in the vasospasm group the possibility of continuous rCBF increase and that of transient rCBF increase in the brain stem were not above 50%. Therefore, the clinical application of Ca antagonists to the treatment of vasospasm after subarachnoid hemorrhage may be difficult. But, on the other hand, it is possible that the administration of Ca antagonists before the appearance of vasospasm would be the prophlaxis for vasospasm after subarachnoid hemorrhage. en-copyright= kn-copyright= en-aut-name=YoshinoKimihiro en-aut-sei=Yoshino en-aut-mei=Kimihiro kn-aut-name=吉野公博 kn-aut-sei=吉野 kn-aut-mei=公博 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳神経外科 en-keyword=cerebral vasospasm kn-keyword=cerebral vasospasm en-keyword=Ca antagonist kn-keyword=Ca antagonist en-keyword=rCBF kn-keyword=rCBF en-keyword=arterial Blood pressure kn-keyword=arterial Blood pressure END start-ver=1.4 cd-journal=joma no-vol=20 cd-vols= no-issue=1 article-no= start-page=35 end-page=43 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19851108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=SAXS Intensity Measurements by Photographic Methods en-subtitle= kn-subtitle= en-abstract= kn-abstract=Photographic method for measurement of small-angl X-ray scattering (SAXS) is improved. Intense pointfocussing incident beam is obtained by using doubly bent crystal monochromator made of aluminium single crystal. Microphotometry and the subsequent calculation to obtain profiles, Guinier and Porod radii, integrated intensities, and so on are facilitated by using microcomputer. Integrated SAXS intensities measured from an Al-Zn alloy which has been treated under the same heat treatment conditions are coincident with one another with probable errors less than ±6 % . Ratio of the integrated intensities obtained from two Al-Zn alloys of different composition is reasonable compared with the quasi-equilibrium phase diagram. en-copyright= kn-copyright= en-aut-name=YamadaMasuo en-aut-sei=Yamada en-aut-mei=Masuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SakakibaraAkira en-aut-sei=Sakakibara en-aut-mei=Akira kn-aut-name=榊原精 kn-aut-sei=榊原 kn-aut-mei=精 aut-affil-num=2 ORCID= en-aut-name=OhtaMutsuo en-aut-sei=Ohta en-aut-mei=Mutsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Industrial Science affil-num=2 en-affil= kn-affil=Department of Industrial Science affil-num=3 en-affil= kn-affil=Department of Industrial Science END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=1 article-no= start-page=11 end-page=21 dt-received= dt-revised= dt-accepted= dt-pub-year=1974 dt-pub=19740720 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Grouth of G.P. zones in Al-Zn alloy en-subtitle= kn-subtitle= en-abstract= kn-abstract=Metastable values of electrical resistivity, P(E)' obtained during isothermal ageing differs in each experimental run even when the conditions of quenching and ageing were carefully kept constant. This phenomenon is considered to result from the competitive growth of G.P.zones. The range of the values of P(E) under the same conditions of heat treatments were examined, and the results obtained are as follows: (1) Metastable values of resistivity, P(E), during ageing at 70℃ after quenching from 300℃ were in rather narrow range. On the other hand, the width of the range obtained during ageing at 50℃ was wide. (2) When the specimens were aged at first at 70℃ until the maximum values of resistivity, P(M), being reached and then aged at 50℃ for long time, the metastable values of resistivity, PE*, were obtained. And the width of discrepancy of values of P(E)* was nearly equal to that of P(E) which was obtained in the case of isothermal ageing at 70℃ after quenching from 300℃. (3) It may be concluded that the width of discrepancy of values of P(E)* becomes smaller since the width of discrepancy of the number of G.P.zones for all experiments which can grow through competitive growth is made small by 70℃ ageing than that immediately after quenching from 300℃. en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=OhtaMutsuo kn-aut-sei=Ohta kn-aut-mei=Mutsuo aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=NagaiMakoto kn-aut-sei=Nagai kn-aut-mei=Makoto aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Industrial Science affil-num=2 en-affil= kn-affil=Graduate School of Industrial Science END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue=1 article-no= start-page=47 end-page=52 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=19710901 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Solubility of Sulphur in a Cadmium Borate Glass and SomeProperties of the Sulphur Containing Glasses en-subtitle= kn-subtitle= en-abstract= kn-abstract=The glass forming limit by substitution of CdS for CdO in a CdO-B(2)0(3) glass was determined by chemical analysis. When x CdS-(60-x)CdO-40B(2)0(3) nominal mixtures in weight ratio were heated at 1100°C in flowing nitrogen gas for 1/2 hour, about 40~50% of mixed CdS and 10-15% of the mixed CdS were evaporated. The limit of nominal composition for glass forming was 10CdS-50CdO-40B(2)0(3) and the corresponding virtual composition after the above heat treatment was found to be 4.9CdS-46.4CdO-48.7B(2)O(3). Further addition of CdS made the melt devitrefied with CdS precipitation. D.C. conductivity measurements revealed that the current density was not linear with respect to the applied voltage, but the resistivity ranged around 10(12)Ω･cm for 4.1CdS-48.8CdO-47.1B(2)O(3) (virtual composition) glass and around 10(11.5)Ω?cm for 4.9CdS-46.4CdO-48.7B(2)O(3) glass. These sulphur containing glasses did not show photoconduction, although CdS-precipitated materials showed slight photoconductivity when disposed in ultra violet radiation. Apparent dielectric constant and tan 8 were also measured as a function of frequency, revealing a moderate dispersion in the CdS-precipitated glasses. en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=TakahashiKatsuaki kn-aut-sei=Takahashi kn-aut-mei=Katsuaki aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=GotoYasumasa kn-aut-sei=Goto kn-aut-mei=Yasumasa aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Industrial Chemistry affil-num=2 en-affil= kn-affil=Department of Industrial Chemistry END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=1 article-no= start-page=134 end-page=137 dt-received= dt-revised= dt-accepted= dt-pub-year=1966 dt-pub=19660331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On the Aging and Precipitation of Al-Ag and Al-Zn Alloys en-subtitle= kn-subtitle= en-abstract= kn-abstract=Polycrystalline specimens of Al-30wt % Ag and Al-30wt % Zn alloys, which were quenched into water from the temperature of solution heat treatment, were annealed at L.T. aging temperatures or reversion temperatures so as to make them contain zones of nearly equal radii for each alloy and various concentrations of solute element in zones. These specimens were cold rolled exactly to 50%, and then annealed at L. T. aging temperatures for varying time. The state of zones and precipitates were investigated by X-ray small-angle scattering photographs. The results obtained were as follows: (1) The precipitation ofγ'-phase began earlier in the specimens of Al-Ag alloy annealed at L. T. aging temperature before cold rolling than in those specimens annealed at reversion temperatures before cold rolling when annealed at L. T. aging temperatures after cold rolling. (2) The rate of precipitation of Zn solid solution in Al-Zn alloy did not depend upon the annealing temperature before cold rolling when cold rolled specimens were annealed at L. T. aging temperature. (3) In Al-Ag alloy, the rate of disappearance of G. P. zones at L. T. aging temperature depends mainly upon the annealing temperature before cold rolling. On the other hand, in Al-Zn alloy, the rate of disappearance of G. P. zones at L. T. aging temperature does not depened upon the annealing temperature before cold rolling. (4) These results may be explained without contradiction considering the relation of structures between matrix and precipitates and the deformation stacking faults. en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=OhtaMutsuo kn-aut-sei=Ohta kn-aut-mei=Mutsuo aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Department of Industrial Science END start-ver=1.4 cd-journal=joma no-vol=29 cd-vols= no-issue= article-no= start-page=11 end-page=15 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200710 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Material recycling of blast furnace slag by phase separation of glass kn-title=ガラスの分相現象を利用した高炉水砕スラグの再資源化技術の開発 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A novel recycling process of blast furnace slag was developed in order to obtain colorless silica-rich solids by using phase separation of borosilicate glass. B(2)O(3) was added to blast furnace slag to promote the phase separation. The slag glasses were heat-treated above glass transition temperatures. The slag glass prepared from blast furnace slag gelled after the heat treatment and the subsequent three types of acid treatment. The ratios of SiO(2) component in the gels were 40 ? 60mass%. On the other hand, phase separation was observed on the surface of the slag glasses prepared from pre-treated slag by 2.5N HCl after the heat treatment. After soaking in acid, they did not gel and changed to insoluble colorless solids. According to compositional analyses, it was found that the insoluble colorless solids contained 70 - 90mass% SiO(2). No colored ions such as Cr, Mn, and Fe were confirmed in the remaining insolubles by optical absorption measurement. Therefore, the colorless silica-rich solids were successfully obtained in the present process. The end products obtained in the present process are expected as material of glass. en-copyright= kn-copyright= en-aut-name=SakidaShinichi en-aut-sei=Sakida en-aut-mei=Shinichi kn-aut-name=崎田真一 kn-aut-sei=崎田 kn-aut-mei=真一 aut-affil-num=1 ORCID= en-aut-name=MikamiSyuuhei en-aut-sei=Mikami en-aut-mei=Syuuhei kn-aut-name=三上修平 kn-aut-sei=三上 kn-aut-mei=修平 aut-affil-num=2 ORCID= en-aut-name=NanbaTokuro en-aut-sei=Nanba en-aut-mei=Tokuro kn-aut-name=難波徳郎 kn-aut-sei=難波 kn-aut-mei=徳郎 aut-affil-num=3 ORCID= en-aut-name=MiuraYoshinari en-aut-sei=Miura en-aut-mei=Yoshinari kn-aut-name=三浦嘉也 kn-aut-sei=三浦 kn-aut-mei=嘉也 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学保健環境センター環境安全部門 affil-num=2 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 affil-num=3 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 affil-num=4 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 en-keyword=Blast furnace slag kn-keyword=Blast furnace slag en-keyword=Recycling kn-keyword=Recycling en-keyword=Colorless silica-rich solids kn-keyword=Colorless silica-rich solids en-keyword=Phase separation kn-keyword=Phase separation en-keyword=Glass kn-keyword=Glass END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=7-8 article-no= start-page=973 end-page=981 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The effect of quercetin on thermotolerance in NIH 3T3 cells : From a view point of cell survival kn-title=NIH 3T3 細胞の温熱耐性に対するケルセチンの作用 第1編 細胞の生存率からみたケルセチンの作用 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The inhibition of thermotolerance development by quercetin was examined in NIH 3T3 cells. The cytotoxicity of quercetin increased with the increase in the concentration (10,100μg/ml) and duration (12,48,72 hours) of treatment. The cell killing effect of heat was not enhanced by quercetin (10μg/ml) itself. Quercetin (10μg/ml) inhibited the proliferation of cells for about 72 hours. Quercetin (10μg/ml) delayed the development of thermotolerance, but did not decrease the degree of maximum thermotolerance. Quercetin (10μg/ml) exibited no effect on the decay of thermotolerance. en-copyright= kn-copyright= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name=黒田昌宏 kn-aut-sei=黒田 kn-aut-mei=昌宏 aut-affil-num=1 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name=平木祥夫 kn-aut-sei=平木 kn-aut-mei=祥夫 aut-affil-num=2 ORCID= en-aut-name=KawasakiShouji en-aut-sei=Kawasaki en-aut-mei=Shouji kn-aut-name=川崎祥二 kn-aut-sei=川崎 kn-aut-mei=祥二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=3 en-affil= kn-affil=岡山大学医療技術短期大学部 en-keyword=ケルセチン kn-keyword=ケルセチン en-keyword=温熱耐性 kn-keyword=温熱耐性 en-keyword=温熱療法 kn-keyword=温熱療法 END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue=1-2 article-no= start-page=137 end-page=144 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Estrogen binding capacity in cytosol fraction of human uterine cervix : effect of estrogen and anti-estrogens kn-title=ヒト子宮頸部組織における細胞質分画エストロゲン結合能とそれに影響する因子について en-subtitle= kn-subtitle= en-abstract= kn-abstract=The estrogen binding activity in the human uterine cervix was measured, and the effect of natural and synthesized steroids on the activities was determined. To assay the estrogen binding activity, the asmple was incubated with 10nM [(3)H] estradiol-17β at 30℃ for 2 hours. Then dextran-coated charcoal (DCC) was added to a final concentration of 0.5% to separate bound/free (B/F) estradiol. Estrogen binding activity was determined by subtracting the activity found in the heated sample from the corresponding activity in the untreated sample. For this purpose it was found appropriate to heat the sample at 40℃ for 60 minutes. The dissociation constant obtained from the Scatchard plot analysis was : kd=2.0×10(-9)M. A variety of steroids at the same molar concentration (1.0 μM) were added to the sample to determine their effects on the estrogen binding activity. For binding with [(3)H] estradiol-17β, the synthetic estrogens were strongly inhibitory, the anti-estrogen agents were strong-ly to moderately inhibitory. Dannazol, which has been used for the treatment of en-dometriosis, was found to be as effective as androgens. All of those inhibitory effects occur-red in a non-competitive manner. en-copyright= kn-copyright= en-aut-name=KobayashiShunzo en-aut-sei=Kobayashi en-aut-mei=Shunzo kn-aut-name=小林俊三 kn-aut-sei=小林 kn-aut-mei=俊三 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部産科婦人科学教室 en-keyword=uterine cervix kn-keyword=uterine cervix en-keyword=estrogen receptor inhibition kn-keyword=estrogen receptor inhibition END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=7-8 article-no= start-page=859 end-page=867 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Persistent herpes simplex virus type 2 infection in a hamster kidney cell line with a characteristic to grow at a high culture temperature kn-title=ハムスター腎臓由来株化細胞の高温馴化亜株における単純ヘルペスウイルス2型の持続感染 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A subline of the baby hamster kidney cell line (BHK-21), which was adapted to grow at 41℃ and named BHK-21H, was used to examine the effect of pre-treatment of host cells with a high culture temperature on the growth of herpes simplex virus (HSV). BHK-21H cells were infected with HSV type 2 (HSV-2) at 41℃ and maintained for 7 days at the same temperature. When these cells were further cultivated after shift-down to 36℃, persistent infections were established. These infections were characterized by a carrier culture state ; the infected cultures were composed of infected and non-infected cells, and cell destruction and regrowth of cells caused a balanced state of culture. Two to 3 months after the establishment of the persistent infections, virus production in the cultures stopped spontaneously and colonial regrowth of uninfected cells was observed. A subline (BHK-21R) which was cured of the persistent infection was obtained. Cells of this subline showed resistance to reinfection of HSV. Persistent HSV-2 Infection was also established by pre-treatment of BHK-21H cells at 41℃ before virus infection and maintenance of the infected cultures at 36℃. These findings indicate that establishment of the persistent HSV-2 infection is mainly attributable to reduced permissiveness of BHK-21H cells for virus growth caused after heat treatment of the cells. en-copyright= kn-copyright= en-aut-name=KitamuraTetsuro en-aut-sei=Kitamura en-aut-mei=Tetsuro kn-aut-name=喜多村哲朗 kn-aut-sei=喜多村 kn-aut-mei=哲朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科学教室 en-keyword=herpes simplex virus kn-keyword=herpes simplex virus en-keyword=persistent infection kn-keyword=persistent infection en-keyword=supraoptimal temperature kn-keyword=supraoptimal temperature en-keyword=carrier culture state kn-keyword=carrier culture state en-keyword=permissiveness kn-keyword=permissiveness END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=3-4 article-no= start-page=401 end-page=413 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Kinetics of heat shock protein 72 in HeLa cells after treatment with heating and/or anticancer agents analyzed by a laser cytometer kn-title=レーザーサイトメーターを用いたheat shock protein 72の加温および抗癌剤添加による動態の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Heat shock proteins (HSPs) are regarded as the proteins most related to the development of thermotolerance. Recently, not only their role in thermotolerance, but also their role in resistance to anticancer agents is gathering concern. In this study, the kinetics of hsp 72 in HeLa cells treated with heating and/or anticancer agents were studied. Hsp 72 was immuno-stained by the indirect fluorescent technique using a monoclonal anti-hsp 72 antibody (Amer-sham). The staining pattern was observed and analyzed using a laser cytometer, ACAS 570 (Meridian). Hsp 72 was normally found in the cytoplasm at 37℃ and moved rapidly into the nucleus with heating at 43℃ for 2 hours. It then returned to the cytoplasm 4 to 6 hours after heating. The hsp 72 content reached a peak at 8 hours after heating. Hsp 72 was induced in all cells treated with cisplation, adriamycin, peplomycin, or etoposide for 48 hours. In the cells treated with both heating at 43℃ for 2 hours and these anticancer agents, hsp 72 induction was most suppressed by adriamycin. However, translocation of hsp 72 to the nucleus was specific for heating and was not affected by the anticancer agents. By laser cytometry the intracellular localization of hsp 72 and the changes of its content were simultaneously detected, Moreover, the change pattern of hsp 72 content measured by laser cytomtry coincided with that measured by the Western blotting procedure. en-copyright= kn-copyright= en-aut-name=SawaiHideaki en-aut-sei=Sawai en-aut-mei=Hideaki kn-aut-name=澤井秀秋 kn-aut-sei=澤井 kn-aut-mei=秀秋 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部産科婦人科学教室 en-keyword=レーザーサイトメーター kn-keyword=レーザーサイトメーター en-keyword=heat shock proteinｎ kn-keyword=heat shock proteinｎ en-keyword=抗癌剤 kn-keyword=抗癌剤 END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=1-2 article-no= start-page=43 end-page=48 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=199302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Thermotolerance induction by benzalkonium chloride in NIH3T3 cells kn-title=塩化ベンザルコニウムによる温熱耐性の誘導 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The ability of benzalkonium chloride to induce thermotolerance was examined in NIH3T3 cells. Benzalkonium chloride enhanced cytotoxicity as its concentration and administration period increased. The cell survival decreased to 50% of that in the non-treated group by 20min of treatment in 0.002% benzalkonium chloride. Thermotolerance developed during the culture after 20min of treatment with 0.002% benzalkonium chloride. Thermotolerance reached its peak at 15h after treatment and decreased subsequently. At 15h after treatment, the Do value at 45℃ heating, a parameter of thermotolerance was 3.8-fold higher than that of the non-treated group. The thermotolerance induced by 0.002% benzalkonium chloride increased as its treatment period was prolonged. These findings suggested a relationship between thermotolerance induction and the cell membrane damage by benzalkonium chloride. en-copyright= kn-copyright= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name=黒田昌宏 kn-aut-sei=黒田 kn-aut-mei=昌宏 aut-affil-num=1 ORCID= en-aut-name=AsaumiJunichi en-aut-sei=Asaumi en-aut-mei=Junichi kn-aut-name=浅海淳一 kn-aut-sei=浅海 kn-aut-mei=淳一 aut-affil-num=2 ORCID= en-aut-name=NishikawaKoji en-aut-sei=Nishikawa en-aut-mei=Koji kn-aut-name=西川光治 kn-aut-sei=西川 kn-aut-mei=光治 aut-affil-num=3 ORCID= en-aut-name=TanakaSeiryo en-aut-sei=Tanaka en-aut-mei=Seiryo kn-aut-name=田中聖了 kn-aut-sei=田中 kn-aut-mei=聖了 aut-affil-num=4 ORCID= en-aut-name=GaoXian Shu en-aut-sei=Gao en-aut-mei=Xian Shu kn-aut-name=高献書 kn-aut-sei=高 kn-aut-mei=献書 aut-affil-num=5 ORCID= en-aut-name=YamamotoMichinori en-aut-sei=Yamamoto en-aut-mei=Michinori kn-aut-name=山本道法 kn-aut-sei=山本 kn-aut-mei=道法 aut-affil-num=6 ORCID= en-aut-name=MakihataEiichi en-aut-sei=Makihata en-aut-mei=Eiichi kn-aut-name=巻幡栄一 kn-aut-sei=巻幡 kn-aut-mei=栄一 aut-affil-num=7 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name=平木祥夫 kn-aut-sei=平木 kn-aut-mei=祥夫 aut-affil-num=8 ORCID= en-aut-name=KawasakiShoji en-aut-sei=Kawasaki en-aut-mei=Shoji kn-aut-name=川崎祥二 kn-aut-sei=川崎 kn-aut-mei=祥二 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=8 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=9 en-affil= kn-affil=岡山大学医療技術短期大学部診療放射線技術学科 en-keyword=温熱療法 kn-keyword=温熱療法 en-keyword=温熱耐性 kn-keyword=温熱耐性 en-keyword=塩化ベンザルコニウム kn-keyword=塩化ベンザルコニウム END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=7-8 article-no= start-page=771 end-page=778 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=199308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Flowcytometric studies of effects of heat on DNA synthesis in different thermosensitivities mammalian cell lines kn-title=温熱感受性の異なる培養細胞の DNA 合成に対する温熱効果―フローサイトメトリーによる研究― en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effect of heat on DNA synthesis in HeLa S3 cells and L-5 cells was studied using flowcytometry. When D(0) values obtained from survival curves of Ehrlich ascites tumor cells, L-5 cells, HeLa S3 cells and NIH3T3 cells after heating to 43, 44, or 45℃, were compared, HeLa S3 cells were resistant and L-5 cells were sensitive to heating. During heating at 43℃, DNA synthesis (BUdR uptake) of HeLa S3 cells was resistant compared to that of L-5 cells. When the period of DNA synthesis was divided into 3 fractions (early S, mid S and late S), late S phase was the most sensitive fraction to heating at 43℃ for 60 minutes. From these results, relationship between DNA synthesis and thermocytotoxic effects are discussed. en-copyright= kn-copyright= en-aut-name=TanakaSeiryou en-aut-sei=Tanaka en-aut-mei=Seiryou kn-aut-name=田中聖了 kn-aut-sei=田中 kn-aut-mei=聖了 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部放射線医学教室 en-keyword=Flowcytometry kn-keyword=Flowcytometry en-keyword=DNA synthesis kn-keyword=DNA synthesis en-keyword=Heating kn-keyword=Heating en-keyword=BUdR kn-keyword=BUdR END start-ver=1.4 cd-journal=joma no-vol=116 cd-vols= no-issue=3 article-no= start-page=245 end-page=250 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=肺悪性腫瘍のラジオ波治療 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=金澤右 kn-aut-sei=金澤 kn-aut-mei=右 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯学総合研究所 放射線医学 en-keyword=肺悪性腫瘍 kn-keyword=肺悪性腫瘍 en-keyword=ラジオ波治療 kn-keyword=ラジオ波治療 en-keyword=熱 kn-keyword=熱 en-keyword=凝固壊死 kn-keyword=凝固壊死 en-keyword=CT kn-keyword=CT END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=1 article-no= start-page=61 end-page=67 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=200802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of Medium Shape, Fertilization Methods and Plant Density on the Growth of Pansy Grown in Medium Hardened by Heat Fusion Polyester Fiber without Polyethylene Pot kn-title=培地の形状，施肥方法，栽植密度が熱融着性ポリエステル繊維固化ポットレス培地で育てたパンジーの生育に及ぼす影響 en-subtitle= kn-subtitle= en-abstract=固化培地を利用したポリポットを用いないポットレス栽培において，潅水効率の上昇，施肥の簡易化，藻の発生抑制のため，培地の形状，施肥方法および栽植密度がパンジーの生育に及ぼす影響について検討した．9p角のポット型培地に6×6×深さ3pのくぼみ（ウォータースペース）を培地上部に作成すると，栽培前期の水供給量（灌水後の重量―灌水前の重量）が約10〜25% に多くなった．しかし，栽培中期から後期にかけてはその違いは次第に小さくなり，特に曇雨天時には水供給量に差はみられなくなった．ウォータースペースの有無にかかわらず，ポリポットがあると生育に違いは認めらなかったが，ポットレス区ではウォータースペースによって生育が改善された．元肥量10ｇ/liter 区では生育初期に高濃度障害と思われる症状が見られ，5ｇ/liter 区より生育が抑制された．栽植密度を半分にしたスペーシングによって，草丈はかなり抑制された．スペーシングなしの場合にはポットの有無による差は小さかったが，ポットレス栽培の生育がスペーシング栽培でかなり改善された．ポットレス・スペーシング有・灌水量120ml区の生育が最も旺盛であった．養分量が多いほど灌水量が多いほど，藻の発生が大であったが，スペーシングとポットレスを組み合わせると藻の発生が改善された． kn-abstract=To develop bedding plant production system without polyethylene pots (PP), problems that may be encountered with compacted medium hardened by heat fusion polyester fiber were investigated. Effects of medium shape, fertilization methods and plant density on the growth of pansy in medium without PP were investigated. To improve water usage on compacted medium, a watering space (6×6×depth 3cm) was made on the upper part of the compacted medium (9×9×depth 9cm). In the early stages of the experiment the amount of water supplied per pot (weight after irrigation-weight before irrigation) of medium with water space was 10?25% greater than that of medium without a water space. However, from the mid to late stages of the experiment, the difference gradually declined. The amount of water supplied per pot was similar between with and without water space especially on cloudy or rainy days. The growth of pansies on medium without PP was remarkably greater with water space than without water space. On 10g/liter basal fertilizer treatment, pansies had severe symptoms of fertilizer damage. The growth of pansy on 10g/liter basal fertilizer was inhibited compared to that of 5g/liter basal fertilizer. Spacing treatment was conducted whenever plant density was half. Height of pansy grown with spacing treatment was significantly lower than that without spacing treatment. PP had no effects on growth when pansy was grown without spacing treatment. However, when pansy was grown with spacing treatment, pansy grown without PP had significantly greater growth than with PP. Algal growth on the medium increased with increasing irrigation rate or nutrient rate. Combination of spacing treatment and medium without PP decreased the rate of algal growth on medium. en-copyright= kn-copyright= en-aut-name=GotoTanjuro en-aut-sei=Goto en-aut-mei=Tanjuro kn-aut-name=後藤丹十郎 kn-aut-sei=後藤 kn-aut-mei=丹十郎 aut-affil-num=1 ORCID= en-aut-name=OohashiYuji en-aut-sei=Oohashi en-aut-mei=Yuji kn-aut-name=大橋佑司 kn-aut-sei=大橋 kn-aut-mei=佑司 aut-affil-num=2 ORCID= en-aut-name=ShimizuNozomi en-aut-sei=Shimizu en-aut-mei=Nozomi kn-aut-name=清水希 kn-aut-sei=清水 kn-aut-mei=希 aut-affil-num=3 ORCID= en-aut-name=MorisitaTeruhisa en-aut-sei=Morisita en-aut-mei=Teruhisa kn-aut-name=森下照久 kn-aut-sei=森下 kn-aut-mei=照久 aut-affil-num=4 ORCID= en-aut-name=FujiiKazunori en-aut-sei=Fujii en-aut-mei=Kazunori kn-aut-name=藤井一徳 kn-aut-sei=藤井 kn-aut-mei=一徳 aut-affil-num=5 ORCID= en-aut-name=IshikawaJunya en-aut-sei=Ishikawa en-aut-mei=Junya kn-aut-name=石川順也 kn-aut-sei=石川 kn-aut-mei=順也 aut-affil-num=6 ORCID= en-aut-name=ShimaKohji en-aut-sei=Shima en-aut-mei=Kohji kn-aut-name=島浩二 kn-aut-sei=島 kn-aut-mei=浩二 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=みのる産業(株)植物工学研究所 affil-num=5 en-affil= kn-affil=みのる産業(株)植物工学研究所 affil-num=6 en-affil= kn-affil=兵庫県立農林水産技術総合センター affil-num=7 en-affil= kn-affil=和歌山県農林水産総合技術センター農業試験場 en-keyword=algal kn-keyword=algal en-keyword=bedding plant kn-keyword=bedding plant en-keyword=spacing kn-keyword=spacing en-keyword=water usage kn-keyword=water usage en-keyword=watering space kn-keyword=watering space END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=1 article-no= start-page=119 end-page=125 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=20020322 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Upconversion property and light scattering in Tm(3+)-doped glass-ceramics kn-title=Tm(3+)含有結晶化ガラスのアップコンバージョン特性と光散乱 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Glass-ceramics containing Pb(x)Cd(1-x)F(2) microcrystallites were prepared through heat treatment of Tm(3+)/Yb(3+)doped SiO(2)-Al(2)O(3)-PbF(2) glasses. The crystallite size was controlled by varying the heat-treatment time. By changing glass composition, two types of strong optical scattering, Rayleigh and Mie scattering modes were observed for the glass ceramics. In the case of Rayleigh scattering, the scattering region expanded to the long-wavelength side with increasing the heat-treatment time. On the other hand, in the case of Mie scattering, the region were hardly dependent on wavelength, and visible light was widely scattered. It was argued that the different scattering phenomena were caused by the different size of the crystallites or their morphogical texture. Furthermore, the glass-ceramics with strong optical scattering showed higher upconversion fluorescence intensity than the matrix glass. The mechanisms for the enhanced upconversion due to the scattering were discussed. en-copyright= kn-copyright= en-aut-name=MiuraYoshinari en-aut-sei=Miura en-aut-mei=Yoshinari kn-aut-name=三浦嘉也 kn-aut-sei=三浦 kn-aut-mei=嘉也 aut-affil-num=1 ORCID= en-aut-name=DingYong en-aut-sei=Ding en-aut-mei=Yong kn-aut-name=丁勇 kn-aut-sei=丁 kn-aut-mei=勇 aut-affil-num=2 ORCID= en-aut-name=MurataTakashi en-aut-sei=Murata en-aut-mei=Takashi kn-aut-name=村田隆 kn-aut-sei=村田 kn-aut-mei=隆 aut-affil-num=3 ORCID= en-aut-name=HimeiYusuke en-aut-sei=Himei en-aut-mei=Yusuke kn-aut-name=姫井裕助 kn-aut-sei=姫井 kn-aut-mei=裕助 aut-affil-num=4 ORCID= en-aut-name=NanbaTokuro en-aut-sei=Nanba en-aut-mei=Tokuro kn-aut-name=難波徳郎 kn-aut-sei=難波 kn-aut-mei=徳郎 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Optical scattering kn-keyword=Optical scattering en-keyword=Glass-ceramics kn-keyword=Glass-ceramics en-keyword=Oxyfluoride glass kn-keyword=Oxyfluoride glass en-keyword=Microcrystallite kn-keyword=Microcrystallite en-keyword=Upconversion fluorescence kn-keyword=Upconversion fluorescence END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=1 article-no= start-page=13 end-page=16 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19950201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Embryoid and Callus Formation from Eggplant Microspores by Cultrre of Anthers Treated with High and Low Temperatures kn-title=ナスの葯培養によるカルス及び胚様体形成に及ぼす温度処理の影響 en-subtitle= kn-subtitle= en-abstract=ナス（品種’早生真黒’）を供試し、葯培養におけるカルス及び杯様体形成を高めるために高温処理を試みた。培地はMS培地に2,4-Dとkinetinをそれぞれ0.02mg/?、ショ糖を3％、Gelriteを0.2％添加したものとした。葯は11月上旬に採取したものを用いた。カルス形成率は対照では14％であったのに対し、葯置床後35℃で48時間の高温処理を行った場合は28％と約2倍まで向上した。また胚様体は高温処理区のみで得られた。また、夏期に同様の品種、培地を用いて花蕾に対する低温処理（4℃-0,5,10日）の効果について検討した。低温処理の効果は認められず、カルス形成葯率は処理日数が長くなるにつれて低下した。また胚様体はいずれの区でも形成されなかった。 kn-abstract=Anthers containing uninucleate microspores of eggplant cv.'Wase-Shinkuro' were cultured on MS supplemented with 0.02mg/1 2,4-D and kinetin,3% sucrose and 0.2% Gelrite with high and low temprerature treatments to increase number of haploids,in early August and September and on November 4,1992.Anthers plated were kept heated at 35℃ for 48h in early November,and callus and embryoid were formed from microspores.The frequency of anthers foming callus was as high as 28% in treated anthers,but 14% in non-treated ones.Embryoids were obtained only in treated anthers,and the frequency of anthers forming embryoid was 5.1%.On the other hand,low temprerature pretreatments at 4℃ for 0,5 or 10days to flower buds in summer season were ineffective for embryoid formation and inhibitory for callus formation with treatement for langer periods. en-copyright= kn-copyright= en-aut-name=TokumoKenji en-aut-sei=Tokumo en-aut-mei=Kenji kn-aut-name=徳毛謙治 kn-aut-sei=徳毛 kn-aut-mei=謙治 aut-affil-num=1 ORCID= en-aut-name=MurakamiKenji en-aut-sei=Murakami en-aut-mei=Kenji kn-aut-name=村上賢治 kn-aut-sei=村上 kn-aut-mei=賢治 aut-affil-num=2 ORCID= en-aut-name=MatsubaraSachiko en-aut-sei=Matsubara en-aut-mei=Sachiko kn-aut-name=松原幸子 kn-aut-sei=松原 kn-aut-mei=幸子 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=eggplant kn-keyword=eggplant en-keyword=anther culture kn-keyword=anther culture en-keyword=temperature treatments kn-keyword=temperature treatments en-keyword=callus formation kn-keyword=callus formation en-keyword=embryoid formation kn-keyword=embryoid formation END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=1 article-no= start-page=39 end-page=44 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Caracteristics of Bacteriocin Produced by Enterococcus Strain from Meat kn-title=食肉より分離した乳酸球菌が生産するバクテリオシンの抗菌特性 en-subtitle= kn-subtitle= en-abstract=発酵肉製品中の汚染細菌の増殖抑制を目的として、市販の食肉より乳酸菌732株を単離し、バクテリオシン産生能を有する乳酸菌の検索を行った。スクリーニングはペーパーディスク法で行い、被検菌には Staphylococcus aureus IAM1011 と Listeria monocytogenes VTU206 を用いた。スクリーニングによりバクテリオシン様物質生産能を示す菌株は4株で、最も抗菌活性を示す1株についての同定を行った結果、本菌株は Enterococcus faesium C210 と同定した。抗菌活性は対数増殖期の後半から定常期の前半にかけて最大となり、それ以降は低下する傾向を示した。また、Catalaseによる処理では失活しなかったが、Pepsin, Carboxypeptidase, Aminopeptidase, Pronase E およびProteinase K等の蛋白質分解酵素による処理で失活した。熱処理の影響を調べたところ、121℃で15分の条件においても抗菌活性は低下しなかった。pHに対しては、pHが高くなるにつれて抗菌活性が低下した。次に抗菌スペクトルを検討した結果、乳酸菌5株の他に、Staphylocossus aureus IAM1011 と Listeria monocytogenes VTU206 等の食品汚染細菌に対して抗菌活性を示したが、グラム陰性菌に対しては示さなかった。また、食品汚染細菌に対する抗菌作用を調べたところ、pH4.5では静菌作用を示し、pH6.5では殺菌作用を示した。 kn-abstract=To control the growth of foodborne spoilage bacteria in fermentes meat produncts, 732 strains of lactic acid bacteria were isolated from commercial meats, and their inhibitory activities were investigated by paper disc assay using Staphylococcus aureus IAM 1011 and Listeria monocytogenes VTU 206 as indicator starins. Four starins produced antibacterial substances and one of them showed greater activity against two indicator strains. It was identified as Enterococcus faecium, and it was designed as strain C210. Antibacterial activity was high between the late-logarithmic growth phase and early-stationary growth phase in broth media. Activity in the antibacterial substance was not detectable after treatments with proteolysis enzymes such as pepsin, carboxypeptidase, aminopeptidase, pronase E and proteinase K. The bacteiocin was heat-stable and showed greater antibacterial activity at low pH. The substance showed antibacterial activity against 5 species of lactic acid bacteria in addition to Staphylococcus aureus and Listeria monocytogenes, although it was not active against Gram-negative bacteria. It exhibited bacteriostatic action at pH 4.5, and bactericidal action at pH 6.5 against Listeira monocytogenes as an indicator strain. en-copyright= kn-copyright= en-aut-name=OkabeShigeto en-aut-sei=Okabe en-aut-mei=Shigeto kn-aut-name=岡部重人 kn-aut-sei=岡部 kn-aut-mei=重人 aut-affil-num=1 ORCID= en-aut-name=MokJong-Soo en-aut-sei=Mok en-aut-mei=Jong-Soo kn-aut-name=睦宗洙 kn-aut-sei=睦 kn-aut-mei=宗洙 aut-affil-num=2 ORCID= en-aut-name=SewakiTomomitsu en-aut-sei=Sewaki en-aut-mei=Tomomitsu kn-aut-name=瀬脇智満 kn-aut-sei=瀬脇 kn-aut-mei=智満 aut-affil-num=3 ORCID= en-aut-name=KataokaKei en-aut-sei=Kataoka en-aut-mei=Kei kn-aut-name=片岡啓 kn-aut-sei=片岡 kn-aut-mei=啓 aut-affil-num=4 ORCID= en-aut-name=IzumimotoMasatoshi en-aut-sei=Izumimoto en-aut-mei=Masatoshi kn-aut-name=泉本勝利 kn-aut-sei=泉本 kn-aut-mei=勝利 aut-affil-num=5 ORCID= en-aut-name=MiyamotoTaku en-aut-sei=Miyamoto en-aut-mei=Taku kn-aut-name=宮本拓 kn-aut-sei=宮本 kn-aut-mei=拓 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=日本食研株式会社 affil-num=2 en-affil= kn-affil=国立水産振興院 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Enterococuus faecium kn-keyword=Enterococuus faecium en-keyword=meat kn-keyword=meat en-keyword=bacteriocin kn-keyword=bacteriocin en-keyword=Staphylococcus aureus kn-keyword=Staphylococcus aureus en-keyword=Listeria monocytogenes kn-keyword=Listeria monocytogenes END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=20001231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=heat shock proteinに対する抗体は血清によるHelicobacter pyloriの早期除菌判定に有用である kn-title=Antibody to Heat Shock Protein Can Be Used for Early Serological Monitoring of Helicobacter pylori Eradication Treatment en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=柚木直子 kn-aut-sei=柚木 kn-aut-mei=直子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=17 cd-vols= no-issue=1 article-no= start-page=39 end-page=46 dt-received= dt-revised= dt-accepted= dt-pub-year=1961 dt-pub=1961 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Polder Soils in Japan. : XVI. Rapid Method for Determining Oxidizable Sulfur and Change of Soil Reaction of Sea Muds and Polder Soils. kn-title=干拓地土壤に関する研究 (第16報) 海底土及び干拓地土中の酸化性硫黄の含量と土壤反応の変化の迅速測定法について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sea muds and saline polder soils frequently contain appreciable amounts of sulfur in various forms, and in polder soils and transported soils derived from sea muds acid condition sometimes develops through aeration of anaerobic soil. A description is given of practical rapid methods of determining the oxidizable sulfur and change of soil reaction of these soils. Recommended experimental procedures are as follows: A weighed quantity of soil (about 1 - 2 g) is mixed with 10 ml of 30 % H2O2, adjusting the pH to 6.0 - 6.5 with N/10 NaOH and removing the sulfuric acid when contained with Ba (OH)2, and heated for about 1 hour on water bath. After cooling, the contents are filled up to 100 ml and filtered. Soil pH value is determined in this soil suspension by means of the pH meter and glass electrode. Titrable acidity is determined by titrating 50 ml of this filtrate with N/10 NaOH and the result is given in terms of ml N/10 NaOH per 100 g soil. Active oxidizable sulfur is calculated from sulfuric acid equivalent to titrable acidity and given in terms of S mg per 100 g soil. Easily oxidizable sulfur is determined from the difference between the water soluble sulfate of the H2O2-treated soil and that of original soil and given in terms of S mg per 100 g soil. Sulfate is determined volumetrically by Benzidine sulfate method. The reliability of this method was examined with sea muds collected from sea bottom, saline pond soils, transported soils derived from sea muds and saline polder soils. The data obtained showed that saline pond soils and some of polder soils contained appreciable amounts of easily oxidizable sulfur and the pH values of these soils became extremely acid after oxidation with H2O2 and easily oxidizable sulfur of fresh soils amounted to over 80 % of total sulfur determined from the difference between the sulfate content of the aqua regia extract and that of the water extract of the soils. (See Table 1 and 2) A considerable difference was frequently found between active oxidizable sulfur and easily oxidizable sulfur and it was shown that the relative amounts of oxidizable sulfur and easily soluble base compounds determine titrable acidity, active oxidizable sulfur and soil pH value of H2O2-treated soil. (See Table 3) The pH values of transported soils derived from sea muds were found to gradually approach in natural fields to the pH values determined by H2O2-treatment. (See Table 4) en-copyright= kn-copyright= en-aut-name=YonedaShigeo en-aut-sei=Yoneda en-aut-mei=Shigeo kn-aut-name=米田茂男 kn-aut-sei=米田 kn-aut-mei=茂男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 en-keyword=干拓地土壤 kn-keyword=干拓地土壤 en-keyword=海底土 kn-keyword=海底土 en-keyword=酸化性硫黄 kn-keyword=酸化性硫黄 en-keyword=迅速測定法 kn-keyword=迅速測定法 END start-ver=1.4 cd-journal=joma no-vol=16 cd-vols= no-issue=1 article-no= start-page=25 end-page=32 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=1960 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental Studies on the Influence of an Abnormally High Temperature upon Some Biological Characters in Inects Survived the Heat-treatment and Those in Their Offsprings. kn-title=異常高温が生き残り昆虫個体及び其の子孫の生理・生態的性質に及ぼす影響に関する実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=本研究は処理区も対照区も共にアズキゾウムシCallosobruchus chinensisの雄雌一対定温飼育によつて親世代を高温処理した後の生き残り固体及び其の子孫の繁殖能力,生活力及び抵抗力がどの様に変化するかを調べたものである. 1. 繁殖能力として一雌当り産卵数は処理された生きのこり個体では少ないが,その子の世代(F1)では逆に増加する.処理後生きのこり個体より生じたF1世代の性比は雌の割合がかなり少ない.これら子孫(F1及びF2)の発育期間はやや長い傾向を示すがその増加の程度はさほど大きくない. 2. 個体の生活力として発育期間中の発育率及び成虫の寿命を調べた結果,処理された固体の子の代(F1)では前者は普通か幾分高いがその次の代(F2)では低下する.後者は処理されて後生き残つた個体は明らかに長<生きられ,その子の世代(F1)では寿命が短縮する. 3. 抵抗力として成虫の高温抵抗の指標と成虫体重を調べたが,処理した生き残り個体は明らかに高温に対する抵抗力が強い.然しそれらの子の世代(F1)では雄は尚強いが雌はそうでなく寧ろ弱い.体重は処理後生きのこり個体がやや重い様に見えるが大きい差ではなく,又その子孫にも大きい差異は見られない. 4. 親世代の高温処理にもとずく昆虫の諸性質の変動は処理した世代とその子世代に明らかに現われるがその次の代即ちF2世代には一部例外はあるが余り明らかに現われない。 en-copyright= kn-copyright= en-aut-name=KiyokuMasao en-aut-sei=Kiyoku en-aut-mei=Masao kn-aut-name=清久正夫 kn-aut-sei=清久 kn-aut-mei=正夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END