BioMed Central Acta Medica Okayama 7 2006 Simultaneous gene transfer of bone morphogenetic protein (BMP)-2 and BMP-7 by in vivo electroporation induces rapid bone formation and BMP-4 expression EN Mariko Kawai Kazuhisa Bessho Hiroki Maruyama Jun-ichi Miyazaki Toshio Yamamoto Background: Transcutaneous in vivo electroporation is expected to be an effective gene-transfer method for promoting bone regeneration using the BMP-2 plasmid vector. To promote enhanced osteoinduction using this method, we simultaneously transferred cDNAs for BMP-2 and BMP-7, as inserts in the non-viral vector pCAGGS. Methods: First, an in vitro study was carried out to confirm the expression of BMP-2 and BMP-7 following the double-gene transfer. Next, the individual BMP-2 and BMP-7 plasmids or both together were injected into rat calf muscles, and transcutaneous electroporation was applied 8 times at 100 V, 50 msec. Results: In the culture system, the simultaneous transfer of the BMP-2 and BMP-7 genes led to a much higher ALP activity in C2C12 cells than did the transfer of either gene alone. In vivo, ten days after the treatment, soft X-ray analysis showed that muscles that received both pCAGGS-BMP-2 and pCAGGS-BMP-7 had better-defined opacities than those receiving a single gene. Histological examination showed advanced ossification in calf muscles that received the double-gene transfer. BMP-4 mRNA was also expressed, and RT-PCR showed that its level increased for 3 days in a timedependent manner in the double-gene transfer group. Immunohistochemistry confirmed that BMP- 4-expressing cells resided in the matrix between muscle fibers. Conclusion: The simultaneous transfer of BMP-2 and BMP-7 genes using in vivo electroporation induces more rapid bone formation than the transfer of either gene alone, and the increased expression of endogenous BMP-4 suggests that the rapid ossification is related to the induction of BMP-4. No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 6 1988 鳥類の卵殻腺におけるカルシウム分泌細胞 24 27 EN Toshio Yamamoto No potential conflict of interest relevant to this article was reported.

岡山大学医学部附属病院三朝分院 Acta Medica Okayama 0918-7839 70 1999 Churg-Strauss症候群の再燃を来した気管支喘息症例 61 66 EN Seishi Harada Hirofumi Tsugeno Fumihiro Mitsunobu Takashi Mifune Yasuhiro Hosaki Kozo Ashida Makoto Okamoto Shingo Takata Toshio Yamamoto Yoshiro Tanizaki 10.18926/14935 入院中にChurg-Strauss症候群の再燃を来した症例を報告した｡症例は気管支喘息を有する73歳の女性で,持続する両下肢の知覚障害を主訴に当院に入院した｡入院2週頃より微熱,下腹部不快感が続き右下肢のdrop foot,下血を呈した｡末梢血では著明な好酸球増多を認め,内視鏡検査で直腸潰瘍を認めた。3年前の両下肢の知覚障害出現時にも同様の症状を経験しており,Churg-Strauss症候群の再燃と診断し,ステロイド治療を開始することにより新たな後遺症を残すことなく寛解に至った｡Churg-Strauss症候群の発症･再燃の機序を考える上で貴重な症例と考え,文献的考察を加え報告する｡ No potential conflict of interest relevant to this article was reported.