Acta Medica Okayama 27 24 2008 Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway 8739 8747 EN Yasuo Agawa Moustafa Sarhan Yuji Kageyama Kazutaka Akagi Masayoshi Takai Kazuya Hashiyama Tadashi Wada Hiroshi Handa Akihiro Iwamatsu Susumu Hirose Hitoshi Ueda <p>Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-fl. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.</p> No potential conflict of interest relevant to this article was reported.