Author Yamaoka, Kiyonori| Mitsunobu, Fumihiro| Kojima, Shuji| Shibakura, Misako| Kataoka, Takahiro| Hanamoto, Katsumi| Tanizaki, Yoshiro|
Published Date 2005-04-01
Publication Title Journal of Radiation Research
Volume volume46
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/31706
FullText URL fulltext.pdf
Author Shibakura, Misako| Niiya, Kenji| Kiguchi, Toru| Nakata, Yasunari| Tanimoto, Mitsune|
Abstract <p>We previously reported that anthracyclines, which could generate reactive oxygen species (ROS), could induce the urokinase-type plasminogen activator (uPA) gene expression in human RC-K8 malignant lymphoma cells and in H69 small cell lung cancer (SCLC) cells. In screening other uPA-inducible anti-cancer agents, we found that camptothecin (CPT) and its derivative, SN38, could induce uPA in RC-K8 and H69 cells. CPT and SN38, which are also used for the treatment of lymphoma and SCLC, significantly increased the uPA accumulation in the conditioned media of both cells in a dose-dependent manner. The maximum induction of uPA mRNA levels was observed 24 h after stimulation. Pretreatment with pyrrolidine dithiocarbamate (PDTC), an anti-oxidant, inhibited the CPT-induced uPA mRNA expression. Thus, CPT induces uPA through gene expression, and, therefore, CPT may influence the tumor-cell biology by up-regulating the uPA/plasmin system.</p>
Keywords CPT SN38 uPA RC-K8 H69
Amo Type Article
Published Date 2002-10
Publication Title Acta Medica Okayama
Volume volume56
Issue issue5
Publisher Okayama University Medical School
Start Page 223
End Page 227
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12530505
Web of Science KeyUT 000178668100002
Author Fukai, Kiyoko| Katsuda, Toshizo| Kondo, Mari| Shibakura, Misako| Asari, Shoji|
Published Date 2006-03-31
Publication Title 岡山大学医学部保健学科紀要
Volume volume16
Issue issue2
Content Type Others
Author Shibakura, Misako|
Published Date 2003-03-25
Publication Title
Content Type Thesis or Dissertation