岡山医学会Acta Medica Okayama0030-155812722015第56回日本小児血液・がん学会学術集会163166ENMegumiOdaNo potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X6822014Detection of RBM15-MKL1 Fusion Was Useful for Diagnosis and Monitoring of Minimal Residual Disease in Infant Acute Megakaryoblastic Leukemia119123ENAkikoTakedaAkiraShimadaKazukoHamamotoSyuujiYoshinoTomokoNagaiYousukeFujiiMutsukoYamadaYoshimiNakamuraToshiyukiWatanabeYukiWatanabeYukoYamamotoKanaeSakakibaraMegumiOdaTsuneoMorishimaCase Report10.18926/AMO/52408Acute megakaryocytic leukemia (AMKL) with t(1;22)(p13;q13) is a distinct category of myeloid leukemia by WHO classification and mainly reported in infants and young children. Accurate diagnosis of this type of AMKL can be difficult, because a subset of patients have a bone marrow (BM) blast percentage of less than 20% due to BM fibrosis. Therefore, it is possible that past studies have underestimated this type of AMKL. We present here the case of a 4-month-old female AMKL patient who was diagnosed by presence of the RBM15-MKL1 (OTT-MAL) fusion transcript by RT-PCR. In addition, we monitored RBM15-MKL1 fusion at several time points as a marker of minimal residual disease (MRD), and found that it was continuously negative after the first induction chemotherapy even by nested RT-PCR. Detection of the RBM15-MKL1 fusion transcript thus seems to be useful for accurate diagnosis of AMKL with t(1;22)(p13;q13). We recommend that the RBM15-MKL1 fusion transcript be analyzed for all suspected AMKL in infants and young children. Furthermore, monitoring of MRD using this fusion transcript would be useful in treatment of AMKL with t(1;22)(p13;q13).No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155812512013第59回日本小児保健協会学術集会学会報告8788ENMegumiOdaNo potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X6522011Analysis of the Factors That Affect Dental Health Behaviour and Attendance at Scheduled Dental Check-ups Using the PRECEDE-PROCEED Model7180ENKimikoSatoMegumiOdaOriginal Article10.18926/AMO/45265A questionnaire survey was administered to 317 parents who attended infant health check-ups in City B, Okayama Prefecture between October, 2008 and March, 2009. The questionnaire survey studied 7 factors based on the PRECEDE-PROCEED Model. We analysed factors that affected oral health behaviour and attendance at scheduled dental health check-ups. The survey containing 22 items concerning matters such as 'QOL' and 'health problems' was posted to parents and guardians in advance, and then collected on the day of the medical check-up. The collected data was analysed using the t-test and Pearson's correlation coefficient, following which we conducted a covariance structure analysis. The results showed that dental health behaviour was directly affected by reinforcing factors, and indirectly associated with enabling and predisposing factors influenced by reinforcing factors. It was also shown that predisposing factors and oral health behaviour were associated with attendance at scheduled oral health check-ups. The results indicated that strengthening oral health education by sharing knowledge that acts as predisposing factors and introducing adaptations of oral health behaviour that that fit individual lives will lead to improved attendance at scheduled dental health check-ups.No potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X4121987Cell-mediated cytotoxicity-supporting activity of various human gammaglobulin preparations.7179ENHiroshiWakiguchiMikiyaFujiedaKenjiMatsumotoYujiOharaYoshioKuroiwaAkikoWakiguchiTaisukeShiraishiMegumiOdaTakanobuKurashigeIsamuKitamuraArticle10.18926/AMO/31747<p>Antibody activity, especially that involved in the reaction of antibody-dependent cell-mediated cytotoxicity (ADCC), of five commercially available human gammaglobulin preparations (standard, pepsin-treated, plasmin-treated, polyethylene glycol-fractionated and S-sulfonated gammaglobulin) was measured. All these gammaglobulin preparations had high titers of hemagglutination inhibition and neutralizing antibody against measles virus. In ADCC reaction, the pepsin-treated gammaglobulin preparation showed no antibody activity. The standard gammaglobulin preparation showed weak activity only when highly diluted. The remaining three preparations showed high activity. Though the S-sulfonated gammaglobulin preparation showed no activity in ADCC reaction, it showed high activity after reconversion by means of oxidation and reduction in vitro. The plasmin-treated gammaglobulin preparation showed greater activity than the polyethylene glycol-fractionated preparation of the optimal concentration. In ADCC tests using the plasmin-treated gammaglobulin preparation, K cell activity was strongly inhibited by Hg (thimerosal), while, in those using the standard gammaglobulin preparation, the activity was hardly influenced by Hg, suggesting that the low ADCC activity of the standard gammaglobulin preparation of high concentrations was due to the inhibitory effect of aggregated immunoglobulin G molecules.</p>
No potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X4331989Elevated IgA antibodies to Epstein-Barr virus in children with chronic active Epstein-Barr virus infection.193196ENHiroshiWakiguchiMikiyaFujiedaHaruoKubotaKenjiMatsumotoAkikoWakiguchiTakanobuKurashigeMegumiOdaArticle10.18926/AMO/30885<p>Anti-Epstein-Barr virus (EBV) antibodies were tested in 11 children with chronic active EBV infection. Anti-virus capsid antigen (VCA)-IgG antibody titers ranged from 1:640 to 1:10,240. Anti-VCA-IgM antibody was consistently positive in 5 of the 11 patients; anti-VCA-IgA antibody was consistently positive in 6 of the 10 patients; anti-early antigen (EA)-IgG antibody was consistently positive in 10 of the 11 patients and anti-EA-IgA antibody was consistently positive in 4 out of the 7 patients. Anti-EBV nuclear antigen (EBNA) antibody was not detected in two patients. Consistently positive anti-VCA-IgA- and anti-EA-IgA- antibody may be a characteristic feature of abnormal antibody responses in severe chronic active EBV-infection in childhood.</p>
No potential conflict of interest relevant to this article was reported.Acta Medica Okayama1990単純ヘルペスウイルス感染細胞に対する新生児･乳幼児期の細胞性免疫能に関する研究ENNo potential conflict of interest relevant to this article was reported.