start-ver=1.4
cd-journal=joma
no-vol=18
cd-vols=
no-issue=1
article-no=
start-page=9
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2026
dt-pub=20260105
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Sodium butyrate augments the antibacterial activity of tetracycline against clinical isolates of multidrug-resistant Vibrio cholerae
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background Antibiotic resistance poses a major challenge in treating Vibrio cholerae infections. One promising method to counter resistance is the co-administration of antibiotics with non-antibiotic adjuvants to enhance their efficacy. This study investigated the combined action of sodium butyrate (SB) and tetracycline on tetracycline-resistant V. cholerae strains.<br>
Results The combined activity of SB and antibiotics was assessed on eight V. cholerae clinical isolates using the Fractional Inhibitory Concentration Index (FICI), with SB-Tetracycline showing strong synergy (FICI: 0.09–0.5). Functional and mechanistic studies, including time-kill kinetics, live/dead staining, SEM-based morphological analysis, and fluorometric assays, demonstrated a synergistic antibacterial effect of SB and Tetracycline. This effect was associated with increased membrane permeability, disruption of membrane integrity, dissipation of the proton motive force, and suppression of efflux activity. These changes collectively led to membrane damage, enhanced intracellular accumulation of Tetracycline, decreased intracellular ATP levels, and ultimately, bacterial cell death. Moreover, GM1-CT ELISA and fluorescence microscopy revealed the synergistic anti-virulence activity of the SB- Tetracycline combination. Finally, the combination of SB and Tetracycline showed enhanced efficacy in animal models compared with monotherapy.<br>
Conclusion: The observed SB-Tetracycline synergy provides a promising therapeutic approach to overcome tetracycline resistance in V. cholerae, offering a potential adjunct strategy for the management of antibiotic-resistant cholera infections.
en-copyright=
kn-copyright=

en-aut-name=KunduSushmita
en-aut-sei=Kundu
en-aut-mei=Sushmita
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AluSourin
en-aut-sei=Alu
en-aut-mei=Sourin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SinghAbhishek
en-aut-sei=Singh
en-aut-mei=Abhishek
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=GopeAnimesh
en-aut-sei=Gope
en-aut-mei=Animesh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NandyRanjan Kumar
en-aut-sei=Nandy
en-aut-mei=Ranjan Kumar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MukhopadhyayAsish K.
en-aut-sei=Mukhopadhyay
en-aut-mei=Asish K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ChatterjeeNabendu Sekhar
en-aut-sei=Chatterjee
en-aut-mei=Nabendu Sekhar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=BhattacharyaSushmita
en-aut-sei=Bhattacharya
en-aut-mei=Sushmita
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Division of Biochemistry, ICMR- National Institute for Research in Bacterial Infections
kn-affil=

affil-num=2
en-affil=Division of Biochemistry, ICMR- National Institute for Research in Bacterial Infections
kn-affil=

affil-num=3
en-affil=Division of Biochemistry, ICMR- National Institute for Research in Bacterial Infections
kn-affil=

affil-num=4
en-affil=Division of General Medicine, ICMR- National Institute for Research in Bacterial Infections
kn-affil=

affil-num=5
en-affil=Division of Bacteriology, ICMR- National Institute for Research in Bacterial Infections
kn-affil=

affil-num=6
en-affil=Division of Bacteriology, ICMR- National Institute for Research in Bacterial Infections
kn-affil=

affil-num=7
en-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Division of Biochemistry, ICMR- National Institute for Research in Bacterial Infections
kn-affil=

affil-num=9
en-affil=Division of Biochemistry, ICMR- National Institute for Research in Bacterial Infections
kn-affil=
en-keyword=V. cholerae
kn-keyword=V. cholerae
en-keyword=Sodium butyrate
kn-keyword=Sodium butyrate
en-keyword=Tetracycline
kn-keyword=Tetracycline
en-keyword=Synergy
kn-keyword=Synergy
en-keyword=Antibiotic adjuvant
kn-keyword=Antibiotic adjuvant
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20251231
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Efficient resuscitation of early-stage viable but non-culturable cells of Vibrio cholerae using treatment with proteolytic enzymes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vibrio cholerae, the etiological agent of cholera, is ubiquitous in environmental brackish waters. Exposure to low water temperatures induces the bacterium to enter a viable but non-culturable (VBNC) state. In this study, a stepwise decrease in water temperature to 4°C was found to delay the transition to the non-culturable state compared to an abrupt temperature drop, suggesting that V. cholerae cells partially adapt to low temperatures. V. cholerae VBNC cells maintained at 4°C gradually lost their ability to revert to a culturable state. However, VBNC cells in the early stage of dormancy were efficiently resuscitated following treatment with proteolytic enzymes, including proteinase K. The abundance of culturable V. cholerae cells in brackish estuarine waters was quantified using the most probable number (MPN)–quantitative polymerase chain reaction (qPCR) method. Although culturable cells were undetectable in samples treated with bovine serum albumin, they were estimated at 93 and 1,500 MPN/mL in two water samples collected on different days and pre-incubated with proteinase K. Similarly, the abundance of Vibrio species increased markedly following treatment with this enzyme. Additionally, cells of Vibrio species were enumerated by the plating method using CHROMagar Vibrio plates. Consistent with the results of the MPN–qPCR method, treatment with proteinase K resulted in over a 100-fold increase in colony formation. Collectively, these findings suggest that treatment with proteinase K is effective for resuscitating and quantifying V. cholerae VBNC cells in environmental water samples.
en-copyright=
kn-copyright=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OgasawaraMona
en-aut-sei=Ogasawara
en-aut-mei=Mona
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NiwakiShiho
en-aut-sei=Niwaki
en-aut-mei=Shiho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SugiharaRena
en-aut-sei=Sugihara
en-aut-mei=Rena
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MuzemboBasilua Andre
en-aut-sei=Muzembo
en-aut-mei=Basilua Andre
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ImamuraDaisuke
en-aut-sei=Imamura
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Research Institute of Nursing Care for People and Community, University of Hyogo
kn-affil=

affil-num=6
en-affil=Research Center for Intestinal Health Science, Okayama University
kn-affil=
en-keyword=Vibrio cholerae
kn-keyword=Vibrio cholerae
en-keyword=viable but non-culturable
kn-keyword=viable but non-culturable
en-keyword=VBNC
kn-keyword=VBNC
en-keyword=protease
kn-keyword=protease
en-keyword=proteolytic enzyme
kn-keyword=proteolytic enzyme
END

start-ver=1.4
cd-journal=joma
no-vol=69
cd-vols=
no-issue=12
article-no=
start-page=e00740-25
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20251210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Genomic portrayal of emerging carbapenem-resistant El Tor variant Vibrio cholerae O1
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The escalating prevalence of carbapenem-resistant (CR) enteric pathogens elicits significant challenges to public health management and effective antimicrobial therapy. While carbapenem resistance is rare in Vibrio cholerae O1 (VC), the recent emergence of CR strains reveals a concerning shift in their antimicrobial resistance (AMR) landscape. This study aims to characterize the resistance mechanisms in newly identified El Tor CRVC isolated from cholera patients in Gujarat, India during 2019. Fifty VC isolates were screened for major virulence-associated genes along with the determination of their antibiotic resistance profiles using Kirby-Bauer disk diffusion and MIC assays. Whole-genome sequencing (WGS) was employed to investigate the underlying mechanisms of CR. All the isolates exhibited hypervirulent Haitian alleles of major virulence genes and AMR profiles of typical multidrug resistance (MDR). Strikingly, 12% (6/50) of them were resistant to carbapenems and other antibiotics. Molecular analysis revealed that these CR isolates were clonally related and harbored a 142 kbp IncA/C type conjugative mega-plasmid with several AMR encoding genes, including blaNDM-1, that can be easily transferred to other bacterial species and confer donor AMR patterns. The plasmid’s competence for horizontal gene transfer presents a significant risk of dissemination to other enteric pathogens and thereby may complicate the treatment. This finding emphasizes the urgent need for enhanced genomic surveillance and robust antimicrobial stewardship programs aimed at curbing the spread of CRVC strains and mitigating their impact on cholera treatment and containment strategies.
en-copyright=
kn-copyright=

en-aut-name=ShawSreeja
en-aut-sei=Shaw
en-aut-mei=Sreeja
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=PragasamAgila Kumari
en-aut-sei=Pragasam
en-aut-mei=Agila Kumari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ChowdhuryGoutam
en-aut-sei=Chowdhury
en-aut-mei=Goutam
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SamantaProsenjit
en-aut-sei=Samanta
en-aut-mei=Prosenjit
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=RoyDeboleena
en-aut-sei=Roy
en-aut-mei=Deboleena
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=GhoshDebjani
en-aut-sei=Ghosh
en-aut-mei=Debjani
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=RamamurthyThandavarayan
en-aut-sei=Ramamurthy
en-aut-mei=Thandavarayan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KariaJigna
en-aut-sei=Karia
en-aut-mei=Jigna
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=NinamaGovind
en-aut-sei=Ninama
en-aut-mei=Govind
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=AkedaYukihiro
en-aut-sei=Akeda
en-aut-mei=Yukihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=KoleyHemanta
en-aut-sei=Koley
en-aut-mei=Hemanta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=MukhopadhyayAsish Kumar
en-aut-sei=Mukhopadhyay
en-aut-mei=Asish Kumar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

affil-num=1
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=2
en-affil=V. Ramalingaswami Bhawan, Indian Council of Medical Research
kn-affil=

affil-num=3
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=4
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=5
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=6
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=7
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=8
en-affil=Medical College Baroda
kn-affil=

affil-num=9
en-affil=Medical College Baroda
kn-affil=

affil-num=10
en-affil=Okayama University
kn-affil=

affil-num=11
en-affil=National Institute of Infectious Diseases
kn-affil=

affil-num=12
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=13
en-affil=ICMR-National Institute for Research in Bacterial Infections
kn-affil=
en-keyword=antimicrobial resistance
kn-keyword=antimicrobial resistance
en-keyword=Vibrio cholerae
kn-keyword=Vibrio cholerae
en-keyword=blaNDM-1
kn-keyword=blaNDM-1
en-keyword=carbapenem resistance
kn-keyword=carbapenem resistance
en-keyword=horizontal gene transfer
kn-keyword=horizontal gene transfer
en-keyword=IncA/C plasmid
kn-keyword=IncA/C plasmid
END

start-ver=1.4
cd-journal=joma
no-vol=17
cd-vols=
no-issue=1
article-no=
start-page=2586329
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20251130
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Asiatic acid, a novel ciprofloxacin adjuvant inhibits Shigella flexneri infection
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bacterial infection caused by intracellular pathogens such as Shigella flexneri is a rapidly increasing global health concern that requires urgent and necessary action. The dearth of licensed vaccines against shigellosis and the decline in susceptibility to conventional antibiotics has encouraged the development of new antibiotic principles and drugs. The treatment options are decreasing faster than the discovery rate of new antibacterial agents. Combinatorial approach of antibiotics with non-antibiotic adjuvants is a promising aspect to treat resistant bacterial infections. Asiatic acid, a membrane-disrupting triterpenoid with wide antimicrobial and immunomodulatory properties, can potentiate antibiotics, but the exact mechanisms remain broadly unexplored. Therefore, in this study, we screened the interaction of asiatic acid with several antibiotics. The results showed synergistic interactions of asiatic acid with antibiotics against susceptible and multidrug-resistant S. flexneri clinical isolates. Particularly important was the interaction of asiatic acid with the quinolone antibiotics ciprofloxacin and nalidixic acid. A detailed study showed that combined treatment of asiatic acid with ciprofloxacin inhibited S. flexneri biofilm formation and resistance development. An increase in membrane disruption and depolarization upon co-treatment was evident by surface electron and confocal microscopy. In addition, asiatic acid and ciprofloxacin synergism was identified to inhibit efflux activity and intracellular bacterial viability. However, asiatic acid showed no synergistic toxicity with ciprofloxacin towards mammalian cells. The antibacterial activity was further verified in a S. flexneri infected mice model. Therapeutic benefits were evident with reduced bacterial burden, recovery from intestinal tissue damage and increase in mice survivability. The results showed that this combination can target the bacterial membrane, efflux pump proteins and biofilm formation, thereby preventing resistance development. The combination treatment offers a proof of concept in targeting essential bacterial activities and might be developed into a novel and efficient treatment alternative against S. flexneri.
en-copyright=
kn-copyright=

en-aut-name=MaitraPriyanka
en-aut-sei=Maitra
en-aut-mei=Priyanka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=BhuktaSamhati
en-aut-sei=Bhukta
en-aut-mei=Samhati
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=GopeAnimesh
en-aut-sei=Gope
en-aut-mei=Animesh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KayetPratanu
en-aut-sei=Kayet
en-aut-mei=Pratanu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=BasakSurajit
en-aut-sei=Basak
en-aut-mei=Surajit
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MiyoshiShin-Ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-Ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KitaharaKei
en-aut-sei=Kitahara
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=DuttaShanta
en-aut-sei=Dutta
en-aut-mei=Shanta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=BhattacharyaSushmita
en-aut-sei=Bhattacharya
en-aut-mei=Sushmita
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Division of Biochemistry, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=2
en-affil=Division of Biochemistry, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=3
en-affil=Division of Clinical Medicine, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=4
en-affil=Division of Bioinformatics, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=5
en-affil=Division of Bioinformatics, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=6
en-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=8
en-affil=Department of Bacteriology, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=9
en-affil=Division of Biochemistry, ICMR-National Institute for Research in Bacterial Infections
kn-affil=
en-keyword=Shigella flexneri
kn-keyword=Shigella flexneri
en-keyword=asiatic acid
kn-keyword=asiatic acid
en-keyword=ciprofloxacin
kn-keyword=ciprofloxacin
en-keyword=adjuvant
kn-keyword=adjuvant
en-keyword=membrane damage
kn-keyword=membrane damage
en-keyword=depolarization
kn-keyword=depolarization
en-keyword=nuclear damage
kn-keyword=nuclear damage
en-keyword=efflux inhibitor
kn-keyword=efflux inhibitor
END

start-ver=1.4
cd-journal=joma
no-vol=15
cd-vols=
no-issue=1
article-no=
start-page=24040
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250705
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Lactose fermenting enteroinvasive Escherichia coli from diarrhoeal cases confers enhanced virulence
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Enteroinvasive Escherichia coli (EIEC), known for causing bacillary dysentery akin to Shigella species, comprises both lactose-fermenting (LF) and non-lactose-fermenting (NLF) isolates. While NLF-EIEC is a well-established pathogen associated with acute dysentery and harbours classical Shigella-like virulence factors, the role of LF-EIEC in human disease remains underexplored. In this study, we sought to characterize LF-EIEC clinical isolates and assessed their pathogenic potential in comparison to NLF-EIEC. Among 13,682 diarrhoeal stool specimens, six LF and nine NLF-EIEC were isolated, predominantly belonging to serogroups O28ac, O125, O136, and O152. Unlike other E. coli, all the EIEC isolates were non-motile. Both the types of EIEC had multiple plasmids harbouring several virulence encoding genes (ipaBCD, ial, virF, sig, sepA and ipaH). Resistance to recent generation antibiotics were mostly confined to NLF-EIEC but some of the LF-EIEC were resistant only to ceftriaxone. Higher invasion ability and significant increase in the expression of virulence encoding genes by the LF-EIEC (p < 0.05) were noted during infection to Int407 cell-line. Additionally, LF-EIEC exhibited extensive colonization of the mouse intestine and expressed severe keratoconjunctivitis in guinea pigs. Together, our findings highlight LF-EIEC as an emerging pathogenic variant warranting heightened surveillance and comprehensive investigation to better understand its epidemiological and clinical significance.
en-copyright=
kn-copyright=

en-aut-name=GhoshDebjani
en-aut-sei=Ghosh
en-aut-mei=Debjani
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HalderProlay
en-aut-sei=Halder
en-aut-mei=Prolay
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SamantaProsenjit
en-aut-sei=Samanta
en-aut-mei=Prosenjit
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ChowdhuryGoutam
en-aut-sei=Chowdhury
en-aut-mei=Goutam
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=ShawSreeja
en-aut-sei=Shaw
en-aut-mei=Sreeja
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=BosePuja
en-aut-sei=Bose
en-aut-mei=Puja
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=RoyDeboleena
en-aut-sei=Roy
en-aut-mei=Deboleena
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=RoyNivedita
en-aut-sei=Roy
en-aut-mei=Nivedita
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KitaharaKei
en-aut-sei=Kitahara
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=RamamurthyThandavarayan
en-aut-sei=Ramamurthy
en-aut-mei=Thandavarayan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=KoleyHemanta
en-aut-sei=Koley
en-aut-mei=Hemanta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=DuttaShanta
en-aut-sei=Dutta
en-aut-mei=Shanta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=MukhopadhyayAsish Kumar
en-aut-sei=Mukhopadhyay
en-aut-mei=Asish Kumar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

affil-num=1
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=2
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=3
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=4
en-affil=Collaborative Research Centre of Okayama University for Infectious Diseases, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=5
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=6
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=7
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=8
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=9
en-affil=Collaborative Research Centre of Okayama University for Infectious Diseases, ICMR-National Institute for Research in Bacterial Infections
kn-affil=

affil-num=10
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=11
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=12
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=13
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=

affil-num=14
en-affil=Division of Bacteriology, ICMR-National Institute for Research in Bacterial Infections (ICMR-NIRBI)
kn-affil=
en-keyword=Antibiotic resistance
kn-keyword=Antibiotic resistance
en-keyword=Bacterial infections
kn-keyword=Bacterial infections
en-keyword=Diarrhoea
kn-keyword=Diarrhoea
en-keyword=Enteroinvasive Escherichia coli
kn-keyword=Enteroinvasive Escherichia coli
en-keyword=Keratoconjunctivitis
kn-keyword=Keratoconjunctivitis
en-keyword=Pathogenesis
kn-keyword=Pathogenesis
END

start-ver=1.4
cd-journal=joma
no-vol=149
cd-vols=
no-issue=
article-no=
start-page=13
end-page=16
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=201809
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Functional analysis of N-terminal propeptide in the precursor of Vibrio vulnificus metalloprotease by using cell-free translational system
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vibrio vulnificus is a human pathogen causing fatal septicemia with edematous and hemorrhagic skin damage. Among multiple virulence factors, an extracellular metalloprotease termed as V. vulnificus protease (VVP) is known to play a crucial role in eliciting the skin damage. The mature VVP (413 aa) is composed of two domains, the N-terminal core domain with proteolytic activity and the C-terminal domain mediates efficient attachment to protein substrates. However, VVP is produced as an inactive precursor (609 aa) with a signal peptide (24 aa) and propeptide (172 aa). In order to clarify the function of propeptide, a series of DNA fragments encoding the VVP precursor and its various domains were designed and the proteins were expressed in vitro by using cell-free translational system. The results indicated that the propeptide might function as an intramolecular chaperon to promote the proper folding of both N-terminal and C-terminal domains. The obtained results also suggest that the propeptide, itself was unstable and thus digested easily by the enzymes present in cell lysate used for cell-free system. Additionally, the C-terminal domain in VVP found to inhibit the folding of the N-terminal domain in absence of propeptide.
en-copyright=
kn-copyright=

en-aut-name=KawaseTomoka
en-aut-sei=Kawase
en-aut-mei=Tomoka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MiuraFumi
en-aut-sei=Miura
en-aut-mei=Fumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=DebnathAnusuya
en-aut-sei=Debnath
en-aut-mei=Anusuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ImakuraKinuyo
en-aut-sei=Imakura
en-aut-mei=Kinuyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=Vibrio vulnificus
kn-keyword=Vibrio vulnificus
en-keyword=Protease
kn-keyword=Protease
en-keyword=Propeptide
kn-keyword=Propeptide
en-keyword=Domain
kn-keyword=Domain
en-keyword=Cell-free translational system
kn-keyword=Cell-free translational system
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=12
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20231214
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Complete genomic sequence of Vibrio fluvialis strain IDH5335 isolated from a patient with diarrhea in Kolkata, India
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We isolated a Vibrio fluvialis strain (IDH5335) from a stool sample collected from a patient with diarrhea. In this announcement, we report the complete genomic sequence of this organism, which was obtained by combining Illumina and Oxford Nanopore sequencing data.
en-copyright=
kn-copyright=

en-aut-name=ChowdhuryGoutam
en-aut-sei=Chowdhury
en-aut-mei=Goutam
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KitaharaKei
en-aut-sei=Kitahara
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TaniguchiMakoto
en-aut-sei=Taniguchi
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=UesakaKazuma
en-aut-sei=Uesaka
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MuzemboBasilua Andre
en-aut-sei=Muzembo
en-aut-mei=Basilua Andre
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MitraDebmalya
en-aut-sei=Mitra
en-aut-mei=Debmalya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OhnoAyumu
en-aut-sei=Ohno
en-aut-mei=Ayumu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=RamamurthyThandavarayan
en-aut-sei=Ramamurthy
en-aut-mei=Thandavarayan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=DuttaShanta
en-aut-sei=Dutta
en-aut-mei=Shanta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=MukhopadhyayAsish Kumar
en-aut-sei=Mukhopadhyay
en-aut-mei=Asish Kumar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India at ICMR-NICED
kn-affil=

affil-num=2
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India at ICMR-NICED
kn-affil=

affil-num=3
en-affil=Oral Microbiome Center, Taniguchi Dental Clinic
kn-affil=

affil-num=4
en-affil=Graduate School of Bioagricultural Sciences, Nagoya University
kn-affil=

affil-num=5
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=6
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India at ICMR-NICED
kn-affil=

affil-num=7
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases in India at ICMR-NICED
kn-affil=

affil-num=8
en-affil=ICMR-National Institute of Cholera and Enteric Diseases
kn-affil=

affil-num=9
en-affil=ICMR-National Institute of Cholera and Enteric Diseases
kn-affil=

affil-num=10
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=11
en-affil=ICMR-National Institute of Cholera and Enteric Diseases
kn-affil=
en-keyword=Vibrio fluvialis
kn-keyword=Vibrio fluvialis
en-keyword=diarrhea
kn-keyword=diarrhea
en-keyword=bacteria
kn-keyword=bacteria
en-keyword=genome
kn-keyword=genome
END

start-ver=1.4
cd-journal=joma
no-vol=205
cd-vols=
no-issue=10
article-no=
start-page=346
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230929
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Flavobacterium okayamense sp. nov. isolated from surface seawater
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Strain KK2020170T, a Gram-stain negative, yellow colony-forming bacterium, was isolated from surface seawater sampled in Kojima Bay, Okayama, Japan. Phylogenetic analysis based on the 16S rRNA gene revealed that strain KK2020170T belongs to the genus Flavobacterium, with Flavobacterium haoranii LQY-7T (98.1% similarity) being its closest relative, followed by Flavobacterium sediminis MEBiC07310T (96.9%) and Flavobacterium urocaniciphilum YIT 12746T (96.0%). Whole-genome shotgun sequencing showed that strain KK2020170T, when paralleled with F. haoranii LQY-7 T, had 81.3% average nucleotide identity, and 24.6% in silico DNA–DNA hybridization values, respectively. The DNA G + C content of strain KK2020170T was 31.1 mol%. The most abundant fatty acids (> 10%) of strain KK2020170T were iso-C15: 0, iso-C17: 0 3-OH and iso-C15: 1 G. The dominant respiratory quinone of the strain was menaquinone MK-6. Based on the phylogenetic and phenotypic analysis results, we propose that strain KK2020170T represents a novel species, for which the name Flavobacterium okayamense sp. nov. has been proposed. The type strain is KK2020170T (= ATCC TSD-280 T = NBRC 115344 T).
en-copyright=
kn-copyright=

en-aut-name=KitaharaKei
en-aut-sei=Kitahara
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MuzemboBasilua Andre
en-aut-sei=Muzembo
en-aut-mei=Basilua Andre
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MorohoshiSho
en-aut-sei=Morohoshi
en-aut-mei=Sho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KunihiroTadao
en-aut-sei=Kunihiro
en-aut-mei=Tadao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TazatoNozomi
en-aut-sei=Tazato
en-aut-mei=Nozomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OhnoAyumu
en-aut-sei=Ohno
en-aut-mei=Ayumu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=UesakaKazuma
en-aut-sei=Uesaka
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TaniguchiMakoto
en-aut-sei=Taniguchi
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=TechnoSuruga Laboratory Co., Ltd
kn-affil=

affil-num=4
en-affil=TechnoSuruga Laboratory Co., Ltd
kn-affil=

affil-num=5
en-affil=TechnoSuruga Laboratory Co., Ltd
kn-affil=

affil-num=6
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=Graduate School of Bioagricultural Sciences, Nagoya University
kn-affil=

affil-num=8
en-affil=Oral Microbiome Center, Taniguchi Dental Clinic
kn-affil=

affil-num=9
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=Bacteroidota
kn-keyword=Bacteroidota
en-keyword=Flavobacterium
kn-keyword=Flavobacterium
en-keyword=New taxa
kn-keyword=New taxa
en-keyword=Sea water
kn-keyword=Sea water
END

start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=
article-no=
start-page=521
end-page=525
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220211
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Isolation and identification of soil bacteria resistant to surfactants in washing detergents
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Linear alkylbenzene sulfonate (LAS) and polyoxyethylene lauryl ether (POLE) are the major surfactants in washing detergents. In the present study, we isolated surfactant-resistant bacteria from soil samples collected from a sports ground and a farm field. The samples were treated with 2.0% LAS or POLE at 25°C for 30 min and cultivated on agar plates at 25°C for several days, after which manifold bacterial colonies were isolated. Thereafter, we tested the ability of each bacterial isolate to resist the antibacterial activity of the surfactant. Ten LAS-resistant strains were isolated, and all were found to be Gram-negative bacteria such as Enterobacter and Pseudomonas. On the other hand, 18 POLE-resistant strains were isolated, of which 14 were Gram-positive bacteria including Bacillus and Microbacterium. Notably, one POLE-resistant strain was identified as Bacillus cereus, a potential causative agent for foodborne illness. The genera of LAS- and POLE-resistant bacteria did not overlap. Therefore, the combination of LAS and POLE could be more effective to eliminate soil bacteria from clothes and/or daily necessities.
en-copyright=
kn-copyright=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkuboNaomi
en-aut-sei=Okubo
en-aut-mei=Naomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MitsumoriSatoko
en-aut-sei=Mitsumori
en-aut-mei=Satoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama‐City Okayama Japan
kn-affil=

affil-num=3
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama‐City Okayama Japan
kn-affil=
en-keyword=Surfactant
kn-keyword=Surfactant
en-keyword=Washing detergent
kn-keyword=Washing detergent
en-keyword=Linear alkylbenzene sulfonate
kn-keyword=Linear alkylbenzene sulfonate
en-keyword=Soil bacteria
kn-keyword=Soil bacteria
END

start-ver=1.4
cd-journal=joma
no-vol=28
cd-vols=
no-issue=4
article-no=
start-page=1633
end-page=1639
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201204
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=An extracellular serine protease produced by Vibrio vulnificus NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements.
en-copyright=
kn-copyright=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=JiyouWang
en-aut-sei=Jiyou
en-aut-mei=Wang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatohKeizo
en-aut-sei=Katoh
en-aut-mei=Keizo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SenohMitsutoshi
en-aut-sei=Senoh
en-aut-mei=Mitsutoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MizunoTamaki
en-aut-sei=Mizuno
en-aut-mei=Tamaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=MaeharaYoko
en-aut-sei=Maehara
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci

affil-num=2
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci

affil-num=3
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci

affil-num=4
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci

affil-num=5
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci

affil-num=6
en-affil=
kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
en-keyword=Polymerase chain reaction
kn-keyword=Polymerase chain reaction
en-keyword=Purification
kn-keyword=Purification
en-keyword=Serine protease
kn-keyword=Serine protease
en-keyword=Metalloprotease
kn-keyword=Metalloprotease
en-keyword=Vibrio vulnificus
kn-keyword=Vibrio vulnificus
END

start-ver=1.4
cd-journal=joma
no-vol=125
cd-vols=
no-issue=1
article-no=
start-page=35
end-page=39
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=An epidemiologically rare case of Vibrio vulnificus infection that occurred in October in an inland city of Japan
kn-title=内陸地津山で発症した季節外れのVibrio vulnificus感染症
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　A 68-year-old man with alcohol addiction, who lived in the suburbs of Tsuyama, an inland city located in northeast Okayama prefecture, was transported to the emergency unit of the Tsuyama Central Hospital in a state of cardiopulmonary arrest (CPA). Despite rigorous systemic investigation and treatment, the patient died 2 hours after arrival. After his death, Vibrio vulnificus was isolated from his blood culture.
　Vibrio vulnificus causes fatal infection in humans, usually only in areas located close to the sea where appropriate temperature and suitable salt concentration for its growth are available. Therefore, its occurrence is epidemiologically restricted ; in Japan, the western coastal areas, especially in summers, are reported to be the high-risk regions. This is a rare case because it occurred in a city approximately 50 kilometers from both the Sea of Japan and the Pacific coast of Okayama, and at the end of October in 2011. Economic development and distribution systems have made it possible to transport various food products from coastal areas or abroad to any place in a short time, such that these infections can potentially develop in areas other than expected. We should be aware of the increasing risk of Vibrio vulnificus infection during any season and at any place, especially in patients with abnormal liver function.
en-copyright=
kn-copyright=

en-aut-name=HagiyaHideharu
en-aut-sei=Hagiya
en-aut-mei=Hideharu
kn-aut-name=萩谷英大
kn-aut-sei=萩谷
kn-aut-mei=英大
aut-affil-num=1
ORCID=

en-aut-name=ShiotaSumiko
en-aut-sei=Shiota
en-aut-mei=Sumiko
kn-aut-name=塩田澄子
kn-aut-sei=塩田
kn-aut-mei=澄子
aut-affil-num=2
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=三好伸一
kn-aut-sei=三好
kn-aut-mei=伸一
aut-affil-num=3
ORCID=

en-aut-name=KuroeYasutoshi
en-aut-sei=Kuroe
en-aut-mei=Yasutoshi
kn-aut-name=黒江泰利
kn-aut-sei=黒江
kn-aut-mei=泰利
aut-affil-num=4
ORCID=

en-aut-name=NojimaHiroyoshi
en-aut-sei=Nojima
en-aut-mei=Hiroyoshi
kn-aut-name=野島宏悦
kn-aut-sei=野島
kn-aut-mei=宏悦
aut-affil-num=5
ORCID=

en-aut-name=OtaniShinkichi
en-aut-sei=Otani
en-aut-mei=Shinkichi
kn-aut-name=大谷晋吉
kn-aut-sei=大谷
kn-aut-mei=晋吉
aut-affil-num=6
ORCID=

en-aut-name=SugiyamaJunichi
en-aut-sei=Sugiyama
en-aut-mei=Junichi
kn-aut-name=杉山淳一
kn-aut-sei=杉山
kn-aut-mei=淳一
aut-affil-num=7
ORCID=

en-aut-name=NaitoHiromichi
en-aut-sei=Naito
en-aut-mei=Hiromichi
kn-aut-name=内藤宏道
kn-aut-sei=内藤
kn-aut-mei=宏道
aut-affil-num=8
ORCID=

en-aut-name=KawanishiSusumu
en-aut-sei=Kawanishi
en-aut-mei=Susumu
kn-aut-name=川西進
kn-aut-sei=川西
kn-aut-mei=進
aut-affil-num=9
ORCID=

en-aut-name=HagiokaShingo
en-aut-sei=Hagioka
en-aut-mei=Shingo
kn-aut-name=萩岡信吾
kn-aut-sei=萩岡
kn-aut-mei=信吾
aut-affil-num=10
ORCID=

en-aut-name=MorimotoNaoki
en-aut-sei=Morimoto
en-aut-mei=Naoki
kn-aut-name=森本直樹
kn-aut-sei=森本
kn-aut-mei=直樹
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=2
en-affil=
kn-affil=就実大学薬学部　病原微生物学

affil-num=3
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科　衛生微生物化学

affil-num=4
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=5
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=6
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=7
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=8
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=9
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=10
en-affil=
kn-affil=津山中央病院　救命救急センター

affil-num=11
en-affil=
kn-affil=津山中央病院　救命救急センター
en-keyword=Vibrio vulnificus
kn-keyword=Vibrio vulnificus
END

start-ver=1.4
cd-journal=joma
no-vol=57
cd-vols=
no-issue=6
article-no=
start-page=904
end-page=908
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=201105
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Inactivation of Vibrio vulnificus hemolysin through mutation of the N- or C-terminus of the lectin-like domain
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Vibrio vulnificus is an etiological agent causing serious systemic infections in the immunocompromised humans or cultured eels. This species commonly produces a hemolytic toxin consisting of the cytolysin domain and the lectin-like domain. For hemolysis, the lectin-like domain specifically binds to cholesterol in the erythrocyte membrane, and to form a hollow oligomer, the toxin is subsequently assembled on the membrane. The cytolysin domain is essential for the process to form the oligomer. Three-dimensional structure model revealed that two domains connected linearly and the C-terminus was located near to the joint of the domains. Insertion of amino acid residues between two domains was found to cause inactivation of the toxin. In the C-terminus, deletion, substitution or addition of an amino acid residue also elicited reduction of the activity. However, the cholesterol-binding ability was not affected by the mutations. These results suggest that mutation of the C- or N-terminus of the lectin-like domain may result in blockage of the toxin assembly.
en-copyright=
kn-copyright=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AbeYuki
en-aut-sei=Abe
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SenohMitsutoshi
en-aut-sei=Senoh
en-aut-mei=Mitsutoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MizunoTamaki
en-aut-sei=Mizuno
en-aut-mei=Tamaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MaeharaYoko
en-aut-sei=Maehara
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NakaoHiroshi
en-aut-sei=Nakao
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=4
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=5
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=6
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=Vibrio vulnificus
kn-keyword=Vibrio vulnificus
en-keyword=Hemolysin
kn-keyword=Hemolysin
en-keyword=Cell-free translation
kn-keyword=Cell-free translation
en-keyword=Site-directed mutagenesis
kn-keyword=Site-directed mutagenesis
END

start-ver=1.4
cd-journal=joma
no-vol=143
cd-vols=
no-issue=3
article-no=
start-page=230
end-page=234
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20101015
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Prevalence and antimicrobial resistance of Salmonella in retail foods in northern China
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A total of 387 retail meat, seafood and milk powder samples were collected from nine cities in northern China in 2005 and screened for the presence of Salmonella. Salmonella strains isolated were subjected to serotyping and antimicrobial susceptibility testing. Salmonella was isolated from 81 (20.9%, 81/387) samples and classified into 23 serotypes. The isolates were frequently resistant to sulfamethoxazole (86.4%), sulfamethoxazole/trimethoprim (48.1%), nalidixic acid (30.9%), tetracycline (19.8%), carboxybenzylpenicillin (17.3%), amoxicillin (17.3%) and ampicillin (16.0%). The multiple resistance (resistance to ≥ 3 antibiotics) was found in 29.6% (n = 24) isolates. Additionally, 4 isolates from chicken displayed the ACSSuTNx profile, resistant to ampicillin, chloramphenicol, streptomycin, sulfonamide, tetracycline and nalidixic acid, in particular, strain HBS084 showing the resistance to as many as 20 antibiotics. Salmonella from chicken showed the higher frequency of antimicrobial resistance. Our findings indicate that in northern China food products of animal origin can be a source of exposure for consumers to multiresistant Salmonella strains
en-copyright=
kn-copyright=

en-aut-name=YanHe
en-aut-sei=Yan
en-aut-mei=He
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=LiLin
en-aut-sei=Li
en-aut-mei=Lin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=AlamM. Jahangir
en-aut-sei=Alam
en-aut-mei=M. Jahangir
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ShinodaSumio
en-aut-sei=Shinoda
en-aut-mei=Sumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MiyoshiShin-ichi
en-aut-sei=Miyoshi
en-aut-mei=Shin-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=ShiLei
en-aut-sei=Shi
en-aut-mei=Lei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=College of Light Industry and Food Sciences, South China University of Technology

affil-num=2
en-affil=
kn-affil=College of Light Industry and Food Sciences, South China University of Technology

affil-num=3
en-affil=
kn-affil=Texas Commission on Environmental Quality

affil-num=4
en-affil=
kn-affil=Faculty of Sciences, Okayama University of Science

affil-num=5
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=6
en-affil=
kn-affil=College of Light Industry and Food Sciences, South China University of Technology
en-keyword=Salmonella
kn-keyword=Salmonella
en-keyword=Prevalence
kn-keyword=Prevalence
en-keyword=Retail meats
kn-keyword=Retail meats
en-keyword=Antimicrobial resistance
kn-keyword=Antimicrobial resistance
END

start-ver=1.4
cd-journal=joma
no-vol=22
cd-vols=
no-issue=
article-no=
start-page=81
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2000
dt-pub=200009
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=編集後記
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=MiyoshiShinichi
en-aut-sei=Miyoshi
en-aut-mei=Shinichi
kn-aut-name=三好伸一
kn-aut-sei=三好
kn-aut-mei=伸一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END