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Ojima, Hinako Department of Bacteriology, Academic Field of Health Sciences, Okayama University
Kuraoka, Sakiko Department of Gastroenterology and Hepatology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
Okanoue, Shyoutarou Department of Gastroenterology and Hepatology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
Okada, Hiroyuki Himeji Red Cross Hospital Kaken ID publons researchmap
Gotoh, Kazuyoshi Department of Bacteriology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
Matsushita, Osamu Department of Bacteriology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID researchmap
Watanabe, Akari Department of Oral Health Care and Rehabilitation, Institute of Biomedical Sciences, Graduate School, Tokushima University
Yokota, Kenji Department of Bacteriology, Academic Field of Health Sciences, Okayama University ORCID Kaken ID publons researchmap
Abstract
Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whether the nitrate-reducing bacteria isolated from patients with atrophic gastritis caused by H. pylori infection are risk factors for developing atrophic gastritis and gastric neoplasia. Nitrate-reducing bacteria were isolated from patients with atrophic gastritis caused by H. pylori infection. Among the isolated bacteria, Actinomyces oris, Actinomyces odontolyticus, Rothia dentocariosa, and Rothia mucilaginosa were used in the subsequent experiments. Cytokine inducibility was evaluated in monocytic cells, and mitogen-activated protein kinase (MAPK) activity and cell cycle were assessed in the gastric epithelial cells. The cytotoxicities and neutrophil-inducing abilities of the Actinomyces and Rothia species were enhanced when cocultured with H. pylori. Th1/Th2-related cytokines were also expressed, but their expression levels differed depending on the bacterial species. Moreover, H. pylori and Actinomyces activated MAPK (ERK and p38) and affected cell cycle progression. Some nitrate-reducing bacteria cocultured with H. pylori may promote inflammation and atrophy by inducing cytokine production. In addition, the MAPK activation and cell cycle progression caused by these bacteria can contribute to gastric cancer development.
Keywords
Helicobacter pylori
nitrate-reducing bacteria
IL-8
TNF-alpha
cell cycle
Published Date
2022-12-16
Publication Title
Microorganisms
Volume
volume10
Issue
issue12
Publisher
MDPI
Start Page
2495
ISSN
2076-2607
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
Copyright Holders
© 2022 by the authors.
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isVersionOf https://doi.org/10.3390/microorganisms10122495
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https://creativecommons.org/licenses/by/4.0/