start-ver=1.4
cd-journal=joma
no-vol=144-145
cd-vols=
no-issue=
article-no=
start-page=109001
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=202505
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Investigating the fate of Zirconium-89 labelled antibody in cynomolgus macaques
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Preclinical pharmacokinetic studies of therapeutic antibodies in non-human primates are desired because of the difficulty in extrapolating ADME data from animal models to humans. We evaluated the pharmacokinetics of 89Zr (Zirconium-89) -labelled anti-KLH human IgG and its metabolites to confirm their non-specific/physiological accumulation in healthy cynomolgus macaques. The anti-KLH antibody was used as a negative control, ensuring that the observed distribution reflected general IgG behavior rather than antigen-specific accumulation. This provides a valuable reference for comparing the biodistribution of targeted antibodies.
Methods: Selected IgG was conjugated to desferrioxamine (DFO), labelled with 89Zr, and injected into healthy cynomolgus macaques. PET/CT images at the whole-body level were acquired at different time points, and standard uptake values (SUV) in regions of interest, such as the heart, liver, spleen, kidneys, bone, and muscles, were calculated. The distribution of a shortened antibody variant, 89Zr-labelled Fab, as well as that of [89Zr]Zr-DFO and [89Zr]Zr-oxalate, the expected metabolites of 89Zr- labelled IgG, was also assessed.
Results: After 89Zr-labelled IgG injection, the SUV in the heart, vertebral body, and muscle decreased, in line with the 89Zr concentration decrease in the circulation, whereas radioactivity increased over time in the kidneys and liver. Autoradiography of the renal sections indicated that most of the 89Zr- labelled IgG radioactivity accumulated in the renal cortex. Relatively high accumulation in the kidneys was also observed in 89Zr- labelled Fab-injected macaques, and renal autoradiographs of these animals showed that the renal cortex was the preferred accumulation site. However, [89Zr]Zr-DFO was rapidly excreted into the urine, whereas [89Zr]Zr-oxalate was highly accumulated in the epiphysis of the long bones and vertebral body.
Conclusion: In the non-human primate cynomolgus macaque, 89Zr- labelled IgG accumulated in the kidneys and the liver. However, [89Zr]Zr-DFO and 89Zr did not accumulate in these organs. This preclinical pharmacokinetic study performed with human IgG in a non-human primate model using PET is of great significance as it sheds light on the basic fate and distribution of 89Zr- labelled IgG.
en-copyright=
kn-copyright=
en-aut-name=SasakiTakanori
en-aut-sei=Sasaki
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KimuraSadaaki
en-aut-sei=Kimura
en-aut-mei=Sadaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=NodaAkihiro
en-aut-sei=Noda
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MurakamiYoshihiro
en-aut-sei=Murakami
en-aut-mei=Yoshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=MiyoshiSosuke
en-aut-sei=Miyoshi
en-aut-mei=Sosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=AkehiMasaru
en-aut-sei=Akehi
en-aut-mei=Masaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=OchiaiKazuhiko
en-aut-sei=Ochiai
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=WatanabeMasami
en-aut-sei=Watanabe
en-aut-mei=Masami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=HiguchiTakahiro
en-aut-sei=Higuchi
en-aut-mei=Takahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Astellas Pharma Inc.
kn-affil=
affil-num=3
en-affil=Astellas Pharma Inc.
kn-affil=
affil-num=4
en-affil=Astellas Pharma Inc.
kn-affil=
affil-num=5
en-affil=Astellas Pharma Inc.
kn-affil=
affil-num=6
en-affil=Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=7
en-affil=School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
kn-affil=
affil-num=8
en-affil=Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=9
en-affil=Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=10
en-affil=Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=PET imaging
kn-keyword=PET imaging
en-keyword=Zirconium-89
kn-keyword=Zirconium-89
en-keyword=Therapeutic antibodies
kn-keyword=Therapeutic antibodies
en-keyword=Non-human primates
kn-keyword=Non-human primates
END
start-ver=1.4
cd-journal=joma
no-vol=14
cd-vols=
no-issue=1
article-no=
start-page=60
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250106
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Novel Drug Delivery Particles Can Provide Dual Effects on Cancer "Theranostics" in Boron Neutron Capture Therapy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Boron (B) neutron capture therapy (BNCT) is a novel non-invasive targeted cancer therapy based on the nuclear capture reaction 10B (n, alpha) 7Li that enables the death of cancer cells without damaging neighboring normal cells. However, the development of clinically approved boron drugs remains challenging. We have previously reported on self-forming nanoparticles for drug delivery consisting of a biodegradable polymer, namely, “AB-type” Lactosome? nanoparticles (AB-Lac particles)- highly loaded with hydrophobic B compounds, namely o-Carborane (Carb) or 1,2-dihexyl-o-Carborane (diC6-Carb), and the latter (diC6-Carb) especially showed the “molecular glue” effect. Here we present in vivo and ex vivo studies with human pancreatic cancer (AsPC-1) cells to find therapeutically optimal formulas and the appropriate treatment conditions for these particles. The biodistribution of the particles was assessed by the tumor/normal tissue ratio (T/N) in terms of tumor/muscle (T/M) and tumor/blood (T/B) ratios using near-infrared fluorescence (NIRF) imaging with indocyanine green (ICG). The in vivo and ex vivo accumulation of B delivered by the injected AB-Lac particles in tumor lesions reached a maximum by 12 h post-injection. Irradiation studies conducted both in vitro and in vivo showed that AB-Lac particles-loaded with either 10B-Carb or 10B-diC6-Carb significantly inhibited the growth of AsPC-1 cancer cells or strongly inhibited their growth, with the latter method being significantly more effective. Surprisingly, a similar in vitro and in vivo irradiation study showed that ICG-labeled AB-Lac particles alone, i.e., without any 10B compounds, also revealed a significant inhibition. Therefore, we expect that our ICG-labeled AB-Lac particles-loaded with 10B compound(s) may be a novel and promising candidate for providing not only NIRF imaging for a practical diagnosis but also the dual therapeutic effects of induced cancer cell death, i.e., “theranostics”.
en-copyright=
kn-copyright=
en-aut-name=FithroniAbdul Basith
en-aut-sei=Fithroni
en-aut-mei=Abdul Basith
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=InoueHaruki
en-aut-sei=Inoue
en-aut-mei=Haruki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ZhouShengli
en-aut-sei=Zhou
en-aut-mei=Shengli
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=HakimTaufik Fatwa Nur
en-aut-sei=Hakim
en-aut-mei=Taufik Fatwa Nur
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TadaTakashi
en-aut-sei=Tada
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=SuzukiMinoru
en-aut-sei=Suzuki
en-aut-mei=Minoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=SakuraiYoshinori
en-aut-sei=Sakurai
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=IshimotoManabu
en-aut-sei=Ishimoto
en-aut-mei=Manabu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=YamadaNaoyuki
en-aut-sei=Yamada
en-aut-mei=Naoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=SauriasariRani
en-aut-sei=Sauriasari
en-aut-mei=Rani
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=SauerweinWolfgang A. G.
en-aut-sei=Sauerwein
en-aut-mei=Wolfgang A. G.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=WatanabeKazunori
en-aut-sei=Watanabe
en-aut-mei=Kazunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=OhtsukiTakashi
en-aut-sei=Ohtsuki
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
affil-num=1
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=3
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=4
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=5
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=6
en-affil=Institute for Integrated Radiation and Nuclear Science, Kyoto University
kn-affil=
affil-num=7
en-affil=Institute for Integrated Radiation and Nuclear Science, Kyoto University
kn-affil=
affil-num=8
en-affil=J-BEAM, Inc.
kn-affil=
affil-num=9
en-affil=Nihon Fukushi Fuiin Holding, Co., Ltd.
kn-affil=
affil-num=10
en-affil=Faculty of Pharmacy, Universitas Indonesia
kn-affil=
affil-num=11
en-affil=Deutsche Gesellschaft f?r Bor-Neutroneneinfangtherapie DGBNCT e.V., University Hospital Essen, Klinik f?r Strahlentherapie
kn-affil=
affil-num=12
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=13
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=14
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
en-keyword=boron neutron capture therapy (BNCT)
kn-keyword=boron neutron capture therapy (BNCT)
en-keyword=dual therapeutic effects
kn-keyword=dual therapeutic effects
en-keyword=Lactosome ?
kn-keyword=Lactosome ?
en-keyword=hydrophobic boron compound
kn-keyword=hydrophobic boron compound
en-keyword=neutron irradiation
kn-keyword=neutron irradiation
en-keyword=theranostics
kn-keyword=theranostics
END
start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=20
article-no=
start-page=3307
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221021
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Novel Self-Forming Nanosized DDS Particles for BNCT: Utilizing A Hydrophobic Boron Cluster and Its Molecular Glue Effect
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=BNCT is a non-invasive cancer therapy that allows for cancer cell death without harming adjacent cells. However, the application is limited, owing to the challenges of working with clinically approved boron (B) compounds and drug delivery systems (DDS). To address the issues, we developed self-forming nanoparticles consisting of a biodegradable polymer, namely, "AB-type Lactosome (AB-Lac)" loaded with B compounds. Three carborane isomers (o-, m-, and p-carborane) and three related alkylated derivatives, i.e., 1,2-dimethy-o-carborane (diC1-Carb), 1,2-dihexyl-o-carborane (diC6-Carb), and 1,2-didodecyl-o-carborane (diC12-Carb), were separately loaded. diC6-Carb was highly loaded with AB-Lac particles, and their stability indicated the "molecular glue" effect. The efficiency of in vitro B uptake of diC6-Carb for BNCT was confirmed at non-cytotoxic concentration in several cancer cell lines. In vivo/ex vivo biodistribution studies indicated that the AB-Lac particles were remarkably accumulated within 72 h post-injection in the tumor lesions of mice bearing syngeneic breast cancer (4T1) cells, but the maximum accumulation was reached at 12 h. In ex vivo B biodistribution, the ratios of tumor/normal tissue (T/N) and tumor/blood (T/Bl) of the diC6-Carb-loaded particles remained stably high up to 72 h. Therefore, we propose the diC6-Carb-loaded AB-Lac particles as a promising candidate medicine for BNCT.
en-copyright=
kn-copyright=
en-aut-name=FithroniAbdul Basith
en-aut-sei=Fithroni
en-aut-mei=Abdul Basith
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UjiHirotaka
en-aut-sei=Uji
en-aut-mei=Hirotaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=IshimotoManabu
en-aut-sei=Ishimoto
en-aut-mei=Manabu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=AkehiMasaru
en-aut-sei=Akehi
en-aut-mei=Masaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=OhtsukiTakashi
en-aut-sei=Ohtsuki
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=2
en-affil=Collaborative Research Center for OMIC, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Material Chemistry, Graduate School of Engineering, Kyoto University
kn-affil=
affil-num=4
en-affil=Fukushima SiC Applied Engineering Inc.
kn-affil=
affil-num=5
en-affil=Collaborative Research Center for OMIC, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=6
en-affil=Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=7
en-affil=Department of Cell Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=boron neutron capture therapy (BNCT)
kn-keyword=boron neutron capture therapy (BNCT)
en-keyword=biologically self-degradable amphipathic polymer (Lactosome)
kn-keyword=biologically self-degradable amphipathic polymer (Lactosome)
en-keyword=hydrophobic boron cluster
kn-keyword=hydrophobic boron cluster
en-keyword=carborane isomers or o-carborane alkylated derivatives
kn-keyword=carborane isomers or o-carborane alkylated derivatives
en-keyword=molecular glue effect
kn-keyword=molecular glue effect
END
start-ver=1.4
cd-journal=joma
no-vol=48
cd-vols=
no-issue=4
article-no=
start-page=768
end-page=781
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2007
dt-pub=200704
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The association of C-reactive protein with an oxidative metabolite of LDL and its implication in atherosclerosis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=C-reactive protein (CRP) is one of the strongest independent predictors of cardiovascular disease. We have previously reported that oxidized LDL (oxLDL) interacts with beta 2-glycoprotein I (beta 2GPI), implicating oxLDL/P2GPI complexes as putative autoantigens in autoimmune-mediated atherosclerotic vascular disease. In this study, we investigated the interaction of CRP with oxLDL/beta 2GPI complexes and its association with atherosclerosis in patients with diabetes mellitus (DM). CRP/oxLDL/R2GPI complexes were predominantly found in sera of DM patients with atherosclerosis. In contrast, noncomplexed CRP isoforms were present in sera of patients with acute/chronic inflammation, i.e., various pyrogenic diseases, rheumatoid arthritis (RA), and DM. Immunohistochemistry staining colocalized CRP and beta 2GPI together with oxLDL in carotid artery plaques but not in synovial tissue from RA patients, strongly suggesting that complex formation occurs during the development of adierosclerosis. Serum levels of CRP correlated with soluble forms of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1, and oxLDL/beta 2GPI complexes correlated with total cholesterol and hemoglobin Al c. Thus, the generation of CRP/oxLDL/beta 2GPI complexes seems to be associated with arterial inflammation, hyperglycemia, and hypercholesterolemia. CRP/oxLDL/R2GPI complexes can be distinguished from pyrogenic noncomplexed CRP isoforms and may represent a more specific and predictive marker for atherosclerosis.
en-copyright=
kn-copyright=
en-aut-name=TabuchiMasako
en-aut-sei=Tabuchi
en-aut-mei=Masako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=InoueKatsumi
en-aut-sei=Inoue
en-aut-mei=Katsumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=Usui-KataokaHitomi
en-aut-sei=Usui-Kataoka
en-aut-mei=Hitomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TeramotoMisako
en-aut-sei=Teramoto
en-aut-mei=Misako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=TakasugiKoji
en-aut-sei=Takasugi
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=ShikataKenichi
en-aut-sei=Shikata
en-aut-mei=Kenichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=YamamuraMasahiro
en-aut-sei=Yamamura
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=AndoKenji
en-aut-sei=Ando
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=NishidaKeiichiro
en-aut-sei=Nishida
en-aut-mei=Keiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=KasaharaJunko
en-aut-sei=Kasahara
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=KumeNoriaki
en-aut-sei=Kume
en-aut-mei=Noriaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=LopezLuis R.
en-aut-sei=Lopez
en-aut-mei=Luis R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=MitsudoKazuaki
en-aut-sei=Mitsudo
en-aut-mei=Kazuaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
en-aut-name=NobuyoshiMasakiyo
en-aut-sei=Nobuyoshi
en-aut-mei=Masakiyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=
en-aut-name=YasudaTatsuji
en-aut-sei=Yasuda
en-aut-mei=Tatsuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=
en-aut-name=KitaToru
en-aut-sei=Kita
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=
en-aut-name=MakinoHirofumi
en-aut-sei=Makino
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=
affil-num=1
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Pathology, Kokura Memorial Hospital
kn-affil=
affil-num=3
en-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Pathology, Kokura Memorial Hospital
kn-affil=
affil-num=6
en-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=8
en-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Department of Cardiology, Kokura Memorial Hospital
kn-affil=
affil-num=10
en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=11
en-affil=Department of Internal Medicine, Okayama Central Hospital
kn-affil=
affil-num=12
en-affil=Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine
kn-affil=
affil-num=13
en-affil=Corgenix, Inc.
kn-affil=
affil-num=14
en-affil=Department of Cardiovascular Medicine, Kurashiki Central Hospital
kn-affil=
affil-num=15
en-affil=Department of Cardiology, Kokura Memorial Hospital
kn-affil=
affil-num=16
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=17
en-affil=Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine
kn-affil=
affil-num=18
en-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=19
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=beta 2-glycoprotein I
kn-keyword=beta 2-glycoprotein I
en-keyword=oxidized LDL/beta 2-glycoprotein I complexes
kn-keyword=oxidized LDL/beta 2-glycoprotein I complexes
en-keyword=diabetes mellitus
kn-keyword=diabetes mellitus
en-keyword=oxidized LDL
kn-keyword=oxidized LDL
END
start-ver=1.4
cd-journal=joma
no-vol=44
cd-vols=
no-issue=4
article-no=
start-page=716
end-page=726
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2003
dt-pub=200304
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Circulating oxidized LDL forms complexes with β(2)-glycoprotein I: implication as an atherogenic autoantigen
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=beta(2)-glycoprotein I (beta(2)-GPI) is a major antigen for antiphospholipid antibodies (Abs, aPL) present in patients with antiphospholipid syndrome (APS). We recently reported (I. Lipid Res., 42: 697, 200 1; J Lipid Res., 43: 1486, 2002) that beta(2)-GPI specifically binds to Cu2+-oxidized LDL (oxLDL) and that the beta(2)-GPI ligands are omega-carboxylated 7-ketocholesteryl esters. In the present study, we demonstrate that oxLDL forms stable and nondissociable complexes with beta(2)-GPI in serum, and that high serum levels of the complexes are associated with arterial thrombosis in APS. A conjugated ketone function at the 7-position of cholesterol as well as the omega-carboxyl function of the beta(2)-GPI ligands was necessary for beta(2)-GPI binding. The ligand-mediated noncovalent interaction of beta(2)-GPI and oxLDL undergoes a temperature- and time-dependent conversion to much more stable but readily dissociable complexes in vitro at neutral pH. In contrast, stable and nondissociable beta(2)-GPI-oxLDL complexes were frequently detected in sera from patients with APS and/or systemic lupus erythematodes. Both the presence Of beta(2)-GPI-oxLDL complexes and IgG Abs recognizing these complexes were strongly associated with arterial thrombosis. Further, these same Abs correlated with IgG immune complexes containing beta(2)-GPI or LDL.jlr Thus, the beta(2)-GPI-oxLDL complexes acting as an autoantigen are closely associated with autoimmune-mediated atherogenesis.
en-copyright=
kn-copyright=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KishiMakoto
en-aut-sei=Kishi
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=AtsumiTatsuya
en-aut-sei=Atsumi
en-aut-mei=Tatsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=BertolacciniMaria L.
en-aut-sei=Bertolaccini
en-aut-mei=Maria L.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=MakinoHirofumi
en-aut-sei=Makino
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=SakairiNobuo
en-aut-sei=Sakairi
en-aut-mei=Nobuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=YamamotoItaru
en-aut-sei=Yamamoto
en-aut-mei=Itaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=YasudaTatsuji
en-aut-sei=Yasuda
en-aut-mei=Tatsuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=KhamashtaMunther A.
en-aut-sei=Khamashta
en-aut-mei=Munther A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=HughesGraham R. V.
en-aut-sei=Hughes
en-aut-mei=Graham R. V.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=KoikeTakao
en-aut-sei=Koike
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=VoelkerDennis R.
en-aut-sei=Voelker
en-aut-mei=Dennis R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
affil-num=1
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=2
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=3
en-affil=Department of Medicine II, Hokkaido University Graduate School of Medicine
kn-affil=
affil-num=4
en-affil=Lupus Research Unit, The Rayne Institute, St. Thomas' Hospital London
kn-affil=
affil-num=5
en-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=6
en-affil=Division of Bioscience, Graduate School of Environment Earth Science, Hokkaido University
kn-affil=
affil-num=7
en-affil=Department of Immunochemistry, Faculty of Pharmaceutical Science, Okayama University
kn-affil=
affil-num=8
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=9
en-affil=Lupus Research Unit, The Rayne Institute, St. Thomas' Hospital London
kn-affil=
affil-num=10
en-affil=Lupus Research Unit, The Rayne Institute, St. Thomas' Hospital London
kn-affil=
affil-num=11
en-affil=Department of Medicine II, Hokkaido University Graduate School of Medicine
kn-affil=
affil-num=12
en-affil=Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center
kn-affil=
affil-num=13
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
en-keyword=antiphospholipid syndrome
kn-keyword=antiphospholipid syndrome
en-keyword=arterial thrombosis
kn-keyword=arterial thrombosis
en-keyword=autoantibody
kn-keyword=autoantibody
END
start-ver=1.4
cd-journal=joma
no-vol=43
cd-vols=
no-issue=9
article-no=
start-page=1486
end-page=1495
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=200209
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=ω-Carboxyl variants of 7-ketocholesteryl esters are ligands for β2-glycoprotein I and mediate antibody-dependent uptake of oxidized LDL by macrophages
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=beta(2)-Glycoprotein I (beta(2)-GPI) is a major antigen for anticardiolipin antibodies (aCL, Abs) present in patients with antiphospholipid syndrome. We recently reported that beta(2)-GPI specifically binds to oxidized LDL (oxLDL) and that the beta(2)-GPI's major ligand, oxLig-1 is 7-ketocholesteryl-9-carboxynonanoate (Kobayashi, K, E. Matsuura, Q. P. Liu, J. Furukawa, K. Kaihara, J. Inagaki, T. Atsumi, N. Sakairi, T. Yasuda, D. R. Welker, and T. Koike. 2001. A specific ligand for beta(2)-glycoprotein I mediates autoantibody-dependent uptake of oxidized low density lipoprotein by macrophages. J Lipid Res. 42: 697-709). In the present study, we demonstrate that omega-carboxylated 7-ketocholesteryl esters are critical for beta(2)-GPI binding. A positive ion mass spectrum of a novel ligand, designated oxLig-2, showed fragmented ions at m/z 383 and 441 in the presence of acetone, which share features of oxLig-1 and 7-ketocholesterol. In the negative ion mode, ions at m/z 627, 625, and 243 were observed. oxLig-2 was most likely 7-ketocholesteryl-12-carboxy (keto) dodecanoate. These ligands were recognized by beta(2)-GPI. Liposome binding to macrophages was significantly increased depending on the ligand's concentration, in the presence of beta(2)-GPI and an anti-beta(2)-GPI Ab. Synthesized variant, 7-ketocholesteryl-13-carboxytxidecanoate (13-COOH-7KC), also showed a significant interaction with beta(2)-GPI and a similar binding profile with macrophages. Methylation of the carboxyl function diminished all of the specific ligand interactions with beta(2)-GPI. Thus, omega-carboxyl variants of 7-ketocholesteryl esters can mediate anti-beta(2)-GPI Ab-dependent uptake of oxLDL by macrophages, and autoimmune atherogenesis linked to beta(2)-GPI interaction with oxLDL.
en-copyright=
kn-copyright=
en-aut-name=LiuQingping
en-aut-sei=Liu
en-aut-mei=Qingping
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=FurukawaJun-ichi
en-aut-sei=Furukawa
en-aut-mei=Jun-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=InagakiJunko
en-aut-sei=Inagaki
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=SakairiNobuo
en-aut-sei=Sakairi
en-aut-mei=Nobuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=IwadoAkimasa
en-aut-sei=Iwado
en-aut-mei=Akimasa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=YasudaTatsuji
en-aut-sei=Yasuda
en-aut-mei=Tatsuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KoikeTakao
en-aut-sei=Koike
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=VoelkerDennis R.
en-aut-sei=Voelker
en-aut-mei=Dennis R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=2
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=3
en-affil=Division of Bioscience, Graduate School of Environment Earth Science, Hokkaido University
kn-affil=
affil-num=4
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=5
en-affil=Division of Bioscience, Graduate School of Environment Earth Science, Hokkaido University
kn-affil=
affil-num=6
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=7
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
affil-num=8
en-affil=Department of Medicine II, Hokkaido University Graduate School of Medicine
kn-affil=
affil-num=9
en-affil=Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center
kn-affil=
affil-num=10
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry
kn-affil=
en-keyword=antiphospholipid syndrome
kn-keyword=antiphospholipid syndrome
en-keyword=atherosclerosis
kn-keyword=atherosclerosis
en-keyword=autoantibody
kn-keyword=autoantibody
en-keyword=beta(2)-glycoprotein I
kn-keyword=beta(2)-glycoprotein I
en-keyword=oxidized LDL
kn-keyword=oxidized LDL
en-keyword=omega-oxidation
kn-keyword=omega-oxidation
END
start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=1788
end-page=1798
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Lactosome-Conjugated siRNA Nanoparticles for Photo-Enhanced Gene Silencing in Cancer Cells
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The A3B-type Lactosome comprised of poly(sarcosine)3-block-poly(l-lactic acid), a biocompatible and biodegradable polymeric nanomicelle, was reported to accumulate in tumors in?vivo via the enhanced permeability and retention (EPR) effect. Recently, the cellular uptake of Lactosome particles was enhanced through the incorporation of a cell-penetrating peptide (CPP), L7EB1. However, the ability of Lactosome as a drug delivery carrier has not been established. Herein, we have developed a method to conjugate the A3B-type Lactosome with ATP-binding cassette transporter G2 (ABCG2) siRNA for inducing in?vitro apoptosis in the cancer cell lines PANC-1 and NCI-H226. The L7EB1 peptide facilitates the cellular uptake efficiency of Lactosome but does not deliver siRNA into cytosol. To establish the photoinduced cytosolic dispersion of siRNA, a photosensitizer loaded L7EB1-Lactosome was prepared, and the photosensitizer 5,10,15,20-tetra-kis(pentafluorophenyl)porphyrin (TPFPP) showed superiority in photoinduced cytosolic dispersion. We exploited the combined effects of enhanced cellular uptake by L7EB1 and photoinduced endosomal escape by TPFPP to efficiently deliver ABCG2 siRNA into the cytosol for gene silencing. Moreover, the silencing of ABCG2, a protoporphyrin IX (PpIX) transporter, also mediated photoinduced cell death via 5-aminolevulinic acid (ALA)-mediated PpIX accumulated photodynamic therapy (PDT). The synergistic capability of the L7EB1/TPFPP/siRNA-Lactosome complex enabled both gene silencing and PDT.
en-copyright=
kn-copyright=
en-aut-name=LimMelissa Siaw Han
en-aut-sei=Lim
en-aut-mei=Melissa Siaw Han
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=NishiyamaYuki
en-aut-sei=Nishiyama
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=OhtsukiTakashi
en-aut-sei=Ohtsuki
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WatanabeKazunori
en-aut-sei=Watanabe
en-aut-mei=Kazunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KobuchiHirotsugu
en-aut-sei=Kobuchi
en-aut-mei=Hirotsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=
kn-affil=
affil-num=2
en-affil=
kn-affil=
affil-num=3
en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=4
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=5
en-affil=
kn-affil=
affil-num=6
en-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=7
en-affil=
kn-affil=
en-keyword=Lactosome
kn-keyword=Lactosome
en-keyword=ABCG2
kn-keyword=ABCG2
en-keyword=siRNA
kn-keyword=siRNA
en-keyword=Cancer
kn-keyword=Cancer
en-keyword=siRNA delivery
kn-keyword=siRNA delivery
en-keyword=Photodynamic therapy
kn-keyword=Photodynamic therapy
en-keyword=Polymeric micelle
kn-keyword=Polymeric micelle
en-keyword=Photosensitizer
kn-keyword=Photosensitizer
en-keyword=Photochemical internalization
kn-keyword=Photochemical internalization
END
start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=2
article-no=
start-page=158
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210218
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Novel 89Zr-labeled DDS Device Utilizing Human IgG Variant (scFv): “Lactosome” Nanoparticle-Based Theranostics for PET Imaging and Targeted Therapy
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=“Theranostics,” a new concept of medical advances featuring a fusion of therapeutic and diagnostic systems, provides promising prospects in personalized medicine, especially cancer. The theranostics system comprises a novel 89Zr-labeled drug delivery system (DDS), derived from the novel biodegradable polymeric micelle, “Lactosome” nanoparticles conjugated with specific shortened IgG variant, and aims to successfully deliver therapeutically effective molecules, such as the apoptosis-inducing small interfering RNA (siRNA) intracellularly while offering simultaneous tumor visualization via PET imaging. A 27 kDa-human single chain variable fragment (scFv) of IgG to establish clinically applicable PET imaging and theranostics in cancer medicine was fabricated to target mesothelin (MSLN), a 40 kDa-differentiation-related cell surface glycoprotein antigen, which is frequently and highly expressed by malignant tumors. This system coupled with the cell penetrating peptide (CPP)-modified and photosensitizer (e.g., 5, 10, 15, 20-tetrakis (4-aminophenyl) porphyrin (TPP))-loaded Lactosome particles for photochemical internalized (PCI) driven intracellular siRNA delivery and the combination of 5-aminolevulinic acid (ALA) photodynamic therapy (PDT) offers a promising nano-theranostic-based cancer therapy via its targeted apoptosis-inducing feature. This review focuses on the combined advances in nanotechnology and material sciences utilizing the “89Zr-labeled CPP and TPP-loaded Lactosome particles” and future directions based on important milestones and recent developments in this platform.
en-copyright=
kn-copyright=
en-aut-name=LimMelissa Siaw Han
en-aut-sei=Lim
en-aut-mei=Melissa Siaw Han
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=OhtsukiTakashi
en-aut-sei=Ohtsuki
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TakenakaFumiaki
en-aut-sei=Takenaka
en-aut-mei=Fumiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=AkehiMasaru
en-aut-sei=Akehi
en-aut-mei=Masaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=UjiHirotaka
en-aut-sei=Uji
en-aut-mei=Hirotaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=KobuchiHirotsugu
en-aut-sei=Kobuchi
en-aut-mei=Hirotsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=SasakiTakanori
en-aut-sei=Sasaki
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=OzekiEiichi
en-aut-sei=Ozeki
en-aut-mei=Eiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Cell Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=3
en-affil=Collaborative Research Centre for OMIC, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=Collaborative Research Centre for OMIC, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=5
en-affil=Collaborative Research Centre for OMIC, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=6
en-affil=Department of Material Chemistry, Graduate School of Engineering, Kyoto University
kn-affil=
affil-num=7
en-affil=Department of Cell Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=8
en-affil=Collaborative Research Centre for OMIC, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=9
en-affil=Technology Research Laboratory, Shimadzu Corporation
kn-affil=
affil-num=10
en-affil=Department of Cell Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=theranostics
kn-keyword=theranostics
en-keyword=single chain variable fragment of IgG (scFv)
kn-keyword=single chain variable fragment of IgG (scFv)
en-keyword=drug delivery system (DDS)
kn-keyword=drug delivery system (DDS)
en-keyword=photodynamic therapy (PDT)
kn-keyword=photodynamic therapy (PDT)
en-keyword=PET imaging
kn-keyword=PET imaging
en-keyword=accelerated blood clearance (ABC)
kn-keyword=accelerated blood clearance (ABC)
en-keyword=cell penetrating peptide (CPP)
kn-keyword=cell penetrating peptide (CPP)
en-keyword=siRNA
kn-keyword=siRNA
en-keyword=ATP-binding cassette subfamily G member 2 (ABCG2)
kn-keyword=ATP-binding cassette subfamily G member 2 (ABCG2)
END
start-ver=1.4
cd-journal=joma
no-vol=6
cd-vols=
no-issue=12
article-no=
start-page=e05743
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=202012
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Antioxidative attributes of rice bran extracts in ameliorative effects of atherosclerosis-associated risk factors
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Oxidative stress, chronic inflammation, dyslipidemia, hyperglycemia, and shear stress (physical effect) are risk factors associated with the pathogenesis of atherosclerosis. Rice bran, a by-product of rice milling process, is known to house polyphenols and vitamins which exhibit potent antioxidant and anti-inflammatory properties. Through recent emerging knowledge of rice bran in health and wellness, the present study was aimed to assess the ameliorative effects of rice bran extracts (RBE) derived from Japanese colored rice varieties in modulating risk factors of atherosclerosis via in vitro and in vivo study models. Pre-treatment of lipopolysaccharide (LPS)-stimulated murine J774A.1 macrophage-like cells with RBE alleviated nitric oxide (NO) overproduction and downregulated gene expressions of pro-inflammatory modulators: tumor necrosis factor-α (TNF-α), interleukin (IL)-α (IL-1α), IL-1β, IL-6, and inducible nitric oxide synthase (iNOS). In addition, RBE also significantly attenuated LPS-stimulated protein expressions of iNOS, TNF-α, IL-1α, and IL-6 in J774A.1 macrophage-like cells as compared to non-treated LPS control group. In in vivo, 12 weeks of RBE dietary supplementations significantly reduced (p < 0.05) total cholesterol, triglycerides, and pro-atherogenic oxidized LDL/β2-glycoprotein I (oxLDL/β2GPI) complexes at plasma levels, in high fat diet (HFD) induced low density lipoprotein receptor knockout (Ldlr?/-) mice. En face pathological assessments of murine aortas also revealed significant reductions by 38% (p < 0.05) in plaque sizes of RBE-supplemented HFD mice groups as compared to non RBE-supplemented HFD control mice group. Moreover, gene expressions of aortic (iNOS, TNF-α, IL-1β) and hepatic (TNF-α, IL-1α, IL-1β) pro-inflammatory modulators were also downregulated in RBE-supplemented mice groups. Present study has revealed the potent health attributes and application of RBE as a dietary supplement to attenuate risks of inadvertent oxidative damage and chronic inflammation underlying the pathogenesis of atherosclerosis. Intrinsically, present preliminary findings may provide global health prospects for future dietary implementation of RBE in management of atherosclerosis.
en-copyright=
kn-copyright=
en-aut-name=XianWen Tan
en-aut-sei=Xian
en-aut-mei=Wen Tan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=LianhuaShen
en-aut-sei=Lianhua
en-aut-mei=Shen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=InagakiJunko
en-aut-sei=Inagaki
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=IdeMasahiro
en-aut-sei=Ide
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=HwangSiaw San
en-aut-sei=Hwang
en-aut-mei=Siaw San
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Collaborative Research Center for OMIC, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Pathophysiology, Zunyi Medical University
kn-affil=
affil-num=4
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=School of Chemical Engineering and Science, Faculty of Engineering, Computing and Science, Swinburne University of Technology Sarawak Campus
kn-affil=
affil-num=7
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=Food science
kn-keyword=Food science
en-keyword=Food analysis
kn-keyword=Food analysis
en-keyword=Rice bran extract (RBE)
kn-keyword=Rice bran extract (RBE)
en-keyword=Functional food
kn-keyword=Functional food
en-keyword=Phytochemicals
kn-keyword=Phytochemicals
en-keyword=Atherosclerosis
kn-keyword=Atherosclerosis
en-keyword=Oxidative stress
kn-keyword=Oxidative stress
en-keyword=Inflammation
kn-keyword=Inflammation
en-keyword=Antioxidant
kn-keyword=Antioxidant
en-keyword=Anti-inflammation
kn-keyword=Anti-inflammation
en-keyword=Oxidized lipoprotein (oxLDL)
kn-keyword=Oxidized lipoprotein (oxLDL)
END
start-ver=1.4
cd-journal=joma
no-vol=6
cd-vols=
no-issue=6
article-no=
start-page=e04114
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200608
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=β2-Glycoprotein I (β2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/β2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both systemic and non-systemic autoimmune diseases. Hence, serological level of oxLDL/β2GPI complexes may represent one crucial clinical parameter for disease prognosis of atherosclerosis-related diseases. Herein, we established a simple, specific and rapid gold nanoparticle (GNP) based lateral flow immunoassay (LFIA) to quantify oxLDL/β2GPI complexes from test samples. Specificities of hybridoma cell-derived monoclonal antibodies against antigen, optimal conditions for conjugation of antibody with GNP, and sensitivity of oxLDL/β2GPI LFIA in comparison to an ELISA-based detection method were assessed accordingly. The established oxLDL/β2GPI LFIA was capable of detecting oxLDL/β2GPI specifically without interference from autoantibodies and solitary components of oxLDL/β2GPI present in test samples. A significant correlation (R2 > 0.8) was also obtained with the oxLDL/β2GPI LFIA when compared to the ELISA-based detection. On the whole, the oxLDL/β2GPI LFIA remains advantageous over the oxLDL/β2GPI ELISA. The unnecessary washing step, short developmental and analytical time support facile and rapid detection of oxLDL/β2GPI as opposed to the laborious ELISA system.
en-copyright=
kn-copyright=
en-aut-name=TanXian Wen
en-aut-sei=Tan
en-aut-mei=Xian Wen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TakenakaFumiaki
en-aut-sei=Takenaka
en-aut-mei=Fumiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TakekawaHironori
en-aut-sei=Takekawa
en-aut-mei=Hironori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Faculty of Medicine, Okayama University
kn-affil=
affil-num=4
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Biological sciences
kn-keyword=Biological sciences
en-keyword=Antibody
kn-keyword=Antibody
en-keyword=Biochemistry
kn-keyword=Biochemistry
en-keyword=Lipid peroxidation
kn-keyword=Lipid peroxidation
en-keyword=Health sciences
kn-keyword=Health sciences
en-keyword=Oxidized LDL (oxLDL)
kn-keyword=Oxidized LDL (oxLDL)
en-keyword=β2-glycoprotein I (β2GPI)
kn-keyword=β2-glycoprotein I (β2GPI)
en-keyword=OxLDL-β2GPI
kn-keyword=OxLDL-β2GPI
en-keyword=Lateral flow immunoassay (LFIA)
kn-keyword=Lateral flow immunoassay (LFIA)
en-keyword=Enzyme-linked immunosorbent assay (ELISA)
kn-keyword=Enzyme-linked immunosorbent assay (ELISA)
en-keyword=Point-of-care
kn-keyword=Point-of-care
END
start-ver=1.4
cd-journal=joma
no-vol=311
cd-vols=
no-issue=
article-no=
start-page=1
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200828
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Identification and visualization of oxidized lipids in atherosclerotic plaques by microscopic imaging mass spectrometry-based metabolomics
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background and aimsDysregulated lipid metabolism has emerged as one of the major risk factors of atherosclerosis. Presently, there is a consensus that oxidized LDL (oxLDL) promotes development of atherosclerosis and downstream chronic inflammatory responses. Due to the dynamic metabolic disposition of lipoprotein, conventional approach to purify bioactive lipids for subsequent comprehensive analysis has proven to be inadequate for elucidation of the oxidized lipids species accountable for pathophysiology of atherosclerotic lesions. Herein, we aimed to utilize a novel mass microscopic imaging technology, coupled with mass spectrometry (MS) to characterize oxidized lipids in atherosclerotic lesions. MethodsWe attempted to use MALDI-TOF-MS and iMScope to identify selected oxidized lipid targets and visualize their respective localizations in study models of atherosclerosis. ResultsBased on the MS analysis, detection of 7-K under positive ionization through product ion peak at m/z 383 [M+H-H2O] indicated the distinctive presence of targeted lipid within Cu2+-oxLDL and Cu2+-oxLDL loaded macrophage-like J774A.1 cell, along with other cholesterol oxidation products. Moreover, the application of two-dimensional iMScope has successfully visualized the localization of lipids in aortic atherosclerotic plaques of the Watanabe heritable hyperlipidemic (WHHL) rabbit. Distinctive lipid distribution profiles were observed in atherosclerotic lesions of different sizes, especially the localizations of lysoPCs in atherosclerotic plaques. ConclusionsTaken together, we believe that both MALDI-TOF-MS and iMScope metabolomics technology may offer a novel proposition for future pathophysiological studies of lipid metabolism in atherosclerosis.
en-copyright=
kn-copyright=
en-aut-name=ShenLianhua
en-aut-sei=Shen
en-aut-mei=Lianhua
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=YamamotoTakushi
en-aut-sei=Yamamoto
en-aut-mei=Takushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TanXian Wen
en-aut-sei=Tan
en-aut-mei=Xian Wen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=OgataKoretsugu
en-aut-sei=Ogata
en-aut-mei=Koretsugu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=AndoEiji
en-aut-sei=Ando
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=OzekiEiichi
en-aut-sei=Ozeki
en-aut-mei=Eiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=Collaborative Research Center (OMIC)
kn-affil=
affil-num=2
en-affil=Analytical & Measuring Instruments Division, Shimadzu Corporation
kn-affil=
affil-num=3
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Analytical & Measuring Instruments Division, Shimadzu Corporation
kn-affil=
affil-num=5
en-affil=Analytical & Measuring Instruments Division, Shimadzu Corporation
kn-affil=
affil-num=6
en-affil=Technology Research Laboratory, Shimadzu Corporation
kn-affil=
affil-num=7
en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=Atherosclerosis
kn-keyword=Atherosclerosis
en-keyword=Low-density lipoprotein (LDL)
kn-keyword=Low-density lipoprotein (LDL)
en-keyword=Oxidized LDL (oxLDL)
kn-keyword=Oxidized LDL (oxLDL)
en-keyword=Oxidized lipids;
kn-keyword=Oxidized lipids;
en-keyword=Imaging mass microscopy (iMScope)
kn-keyword=Imaging mass microscopy (iMScope)
en-keyword=Mass spectroscopy (MS)
kn-keyword=Mass spectroscopy (MS)
END
start-ver=1.4
cd-journal=joma
no-vol=70
cd-vols=
no-issue=1
article-no=
start-page=13
end-page=24
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=201602
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Function of β2-glycoprotein I in Angiogenesis and Its in Vivo Distribution in Tumor Xenografts
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Intact β2-glycoprotein I (iβ2GPI) is a glycoprotein that regulates coagulation and fibrinolysis. Nicked β2GPI (nβ2GPI) possesses an angiogenic property at a relatively low concentration, and an antiangiogenic property at a high concentration. Here we investigated the functions of βi 2GPI and nβ2GPI in vascular endothelial growth factor (VEGF)-A-induced endothelial cell proliferation and tube formation. We used noninvasive PET imaging to analyze the in vivo distribution of intravenously injected β2GPI variants in tumor lesions in mice. iβ2GPI was incubated with plasmin to obtain nβ2GPI, and its N-terminal sequence was analyzed. nβ2GPI had at least one other cleavage site upstream of the β2GPI?s domain V, whereas the former plasmin-cleavage site locates between K317 and T318. Both of intact and nicked β2GPI significantly inhibited the VEGF-A-induced cell proliferation and the tube formation of human umbilical vein endothelial cells (HUVECs). PET imaging visualized considerably distributed intensities of all tested β2GPI variants in tumor lesions of pancreatic tumor cell-xenografts. These results indicate that β2GPI may be physiologically and pathophysiologically important in the regulation of not only coagulation and fibrinolysis, but also angiogenesis.
en-copyright=
kn-copyright=
en-aut-name=Arum Tri Wahyuningsih
en-aut-sei=Arum Tri Wahyuningsih
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=ShenLianhua
en-aut-sei=Shen
en-aut-mei=Lianhua
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KobayashiKazuko
en-aut-sei=Kobayashi
en-aut-mei=Kazuko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=SasakiTakanori
en-aut-sei=Sasaki
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TakenakaFumiaki
en-aut-sei=Takenaka
en-aut-mei=Fumiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=HanadaTakahisa
en-aut-sei=Hanada
en-aut-mei=Takahisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=AkehiMasaru
en-aut-sei=Akehi
en-aut-mei=Masaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=AkahoshiAkiya
en-aut-sei=Akahoshi
en-aut-mei=Akiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=OzekiEiichi
en-aut-sei=Ozeki
en-aut-mei=Eiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=AndoEiji
en-aut-sei=Ando
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Cell Chemistry , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=2
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=3
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=4
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=5
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=6
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=7
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=8
en-affil=
kn-affil=Collaborative Research Center (OMIC), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=9
en-affil=
kn-affil=Technology Research Laboratory, Shimadzu Corporation
affil-num=10
en-affil=
kn-affil=Life Science Business Department, Shimadzu Corporation
affil-num=11
en-affil=
kn-affil=Department of Cell Chemistry , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
en-keyword=β2-glycoprotein I (β2GPI)
kn-keyword=β2-glycoprotein I (β2GPI)
en-keyword=angiogenesis
kn-keyword=angiogenesis
en-keyword=vascular endothelial growth factor-A (VEGF-A)
kn-keyword=vascular endothelial growth factor-A (VEGF-A)
en-keyword=positron emission tomography (PET) imaging
kn-keyword=positron emission tomography (PET) imaging
END
start-ver=1.4
cd-journal=joma
no-vol=125
cd-vols=
no-issue=1
article-no=
start-page=19
end-page=28
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immunity/inflammation-mediated pathophysiological mechanisms of atherosclerosis and clinical applications of antibody technology
kn-title=免疫・炎症が関与する動脈硬化の病態生理学的機序と抗体工学の臨床応用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=松浦栄次
kn-aut-sei=松浦
kn-aut-mei=栄次
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 産学官連携センター
en-keyword=動脈硬化
kn-keyword=動脈硬化
en-keyword=自己免疫
kn-keyword=自己免疫
en-keyword=感染免疫
kn-keyword=感染免疫
en-keyword=インフラマソーム
kn-keyword=インフラマソーム
en-keyword=PET イメージング
kn-keyword=PET イメージング
END
start-ver=1.4
cd-journal=joma
no-vol=179
cd-vols=
no-issue=2
article-no=
start-page=457
end-page=462
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=19940201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Anticardiolipin Antibodies Recognize β(2)-Glycoprotein I Structure Altered by Interacting with an Oxygen Modified Solid Phase Surface
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to beta(2)-glycoprotein I (beta(2)-GPI), which is adsorbed to an oxidized polystyrene surface. Oxygen atoms were introduced on a polystyrene surface by irradiation with electron or gamma-ray radiation. X-ray photoelectron spectroscopy revealed the irradiated surfaces were oxidized to generate C-O and C=O moieties. aCL derived from either APS patients or (NZW x BXSB)F-1 mice bound to beta(2)-GPI coated on the irradiated plates, depending on the radiation dose. Antibody binding to beta(2)-GPI on the irradiated plates was competitively inhibited by simultaneous addition of cardiolipin (CL)-coated latex beads mixed together with beta(2)-GPI but were unaffected by addition of excess beta(2)-GPI, CL micelles, or CL-coated latex beads alone. There was a high correlation between binding values of aCL in sera from 40 APS patients obtained by the anti-beta(2)-GPI enzyme-linked immunosorbent assay (ELISA) using the irradiated plates and those by the beta(2)-GPI-dependent aCL ELISA. Therefore, aCL have specificity for an epitope on beta(2)-GPI. This epitope is expressed by a conformational change occurring when beta(2)-GPI interacts with an oxygen-substituted solid phase surface.
en-copyright=
kn-copyright=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=IgarashiYoshiko
en-aut-sei=Igarashi
en-aut-mei=Yoshiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=YasudaTatsuji
en-aut-sei=Yasuda
en-aut-mei=Tatsuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=TriplettDouglas A.
en-aut-sei=Triplett
en-aut-mei=Douglas A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KoikeTakao
en-aut-sei=Koike
en-aut-mei=Takao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Cell Chemistry, Institute of Molecular and Cellular Biology, Okayama University School of Medicine
affil-num=2
en-affil=
kn-affil=Immunology Laboratory, Diagnostics Division, Yamasa Corporation
affil-num=3
en-affil=
kn-affil=Department of Cell Chemistry, Institute of Molecular and Cellular Biology, Okayama University School of Medicine
affil-num=4
en-affil=
kn-affil=Department of Pathology, Ball Memorial Hospital
affil-num=5
en-affil=
kn-affil=Department of Medicine II, Hokkaido University School of Medicine
END
start-ver=1.4
cd-journal=joma
no-vol=64
cd-vols=
no-issue=2
article-no=
start-page=85
end-page=93
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201004
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=7-Ketocholesterol Induces Cell Apoptosis by Activation of Nuclear Factor kappa B in Mouse Macrophages
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.
en-copyright=
kn-copyright=
en-aut-name=HuangZhenyu
en-aut-sei=Huang
en-aut-mei=Zhenyu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=LiuQingping
en-aut-sei=Liu
en-aut-mei=Qingping
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=LiWenzhe
en-aut-sei=Li
en-aut-mei=Wenzhe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WangRenjun
en-aut-sei=Wang
en-aut-mei=Renjun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=WangDan
en-aut-sei=Wang
en-aut-mei=Dan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=ZhangYingbiao
en-aut-sei=Zhang
en-aut-mei=Yingbiao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=ZhangFan
en-aut-sei=Zhang
en-aut-mei=Fan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=ChiYan
en-aut-sei=Chi
en-aut-mei=Yan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=LiuZhe
en-aut-sei=Liu
en-aut-mei=Zhe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=LiuZibo
en-aut-sei=Liu
en-aut-mei=Zibo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=ZhangQiming
en-aut-sei=Zhang
en-aut-mei=Qiming
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
affil-num=1
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=2
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=3
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=4
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=5
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=6
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=7
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=8
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=9
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
affil-num=10
en-affil=
kn-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=11
en-affil=
kn-affil=The No. 10 Senior High School of Dalian Economical and Technological Development Area
affil-num=12
en-affil=
kn-affil=College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University
en-keyword=7-KC
kn-keyword=7-KC
en-keyword=NF-?B
kn-keyword=NF-?B
en-keyword=apoptosis
kn-keyword=apoptosis
en-keyword=atherosclerosis
kn-keyword=atherosclerosis
END
start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=1
article-no=
start-page=19
end-page=26
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=20020530
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=抗リン脂質抗体症候群
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=松浦栄次
kn-aut-sei=松浦
kn-aut-mei=栄次
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯学総合研究科病態制御科学専攻病態機構学講座（細胞化学分野）
en-keyword=抗リン脂質抗体
kn-keyword=抗リン脂質抗体
en-keyword=抗リン脂質抗体症候群 (APS)
kn-keyword=抗リン脂質抗体症候群 (APS)
en-keyword=β2-グリコプロテインI (β2-GPI)
kn-keyword=β2-グリコプロテインI (β2-GPI)
en-keyword=動脈血栓
kn-keyword=動脈血栓
en-keyword=酸化LDL
kn-keyword=酸化LDL
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=19940325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=抗カルジオリピン抗体は,酸素原子により修飾された固相表面と相互作用することで変化したβ2-グリコプロテインT構造を認識する
kn-title=Anticardiolipin antibodies recognize β(2)-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=MatsuuraEiji
en-aut-sei=Matsuura
en-aut-mei=Eiji
kn-aut-name=松浦栄次
kn-aut-sei=松浦
kn-aut-mei=栄次
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END