start-ver=1.4
cd-journal=joma
no-vol=134
cd-vols=
no-issue=2
article-no=
start-page=76
end-page=78
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220801
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Zebrafish models for cancer research
kn-title=ƒ[ƒuƒ‰ƒtƒBƒbƒVƒ…‚π—p‚’‚½‚ͺ‚ρŒ€‹†
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=HosonoYasuyuki
en-aut-sei=Hosono
en-aut-mei=Yasuyuki
kn-aut-name=Χ–μΛ”V
kn-aut-sei=Χ–μ
kn-aut-mei=Λ”V
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Pharmacology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=‰ͺŽR‘εŠwŠwpŒ€‹†‰@ˆγŽ•–ςŠwˆζ@–ς—Šw
en-keyword=zebrafish
kn-keyword=zebrafish
en-keyword=cancer
kn-keyword=cancer
en-keyword=drug development
kn-keyword=drug development
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=
article-no=
start-page=694018
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220118
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Meclozine Attenuates the MARK Pathway in Mammalian Chondrocytes and Ameliorates FGF2-Induced Bone Hyperossification in Larval Zebrafish
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Meclozine has been developed as an inhibitor of fibroblast growth factor receptor 3 (FGFR3) to treat achondroplasia (ACH). Extracellular signal regulated kinase (ERK) phosphorylation was attenuated by meclozine in FGF2-treated chondrocyte cell line, but the site of its action has not been elucidated. Although orally administered meclozine promoted longitudinal bone growth in a mouse model of ACH, its effect on craniofacial bone development during the early stage remains unknown. Herein, RNA-sequencing analysis was performed using murine chondrocytes from FGF2-treated cultured tibiae, which was significantly elongated by meclozine treatment. Gene set enrichment analysis demonstrated that FGF2 significantly increased the enrichment score of mitogen-activated protein kinase (MAPK) family signaling cascades in chondrocytes; however, meclozine reduced this enrichment. Next, we administered meclozine to FGF2-treated larval zebrafish from 8 h post-fertilization (hpf). We observed that FGF2 significantly increased the number of ossified vertebrae in larval zebrafish at 7 days post-fertilization (dpf), while meclozine delayed vertebral ossification in FGF2-induced zebrafish. Meclozine also reversed the FGF2-induced upregulation of ossified craniofacial bone area, including ceratohyal, hyomandibular, and quadrate. The current study provided additional evidence regarding the inhibitory effect of meclozine on the FGF2-induced upregulation of MAPK signaling in chondrocytes and FGF2-induced development of craniofacial and vertebral bones.
en-copyright=
kn-copyright=

en-aut-name=TakemotoGenta
en-aut-sei=Takemoto
en-aut-mei=Genta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsushitaMasaki
en-aut-sei=Matsushita
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OkamotoTakaaki
en-aut-sei=Okamoto
en-aut-mei=Takaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ItoToshinari
en-aut-sei=Ito
en-aut-mei=Toshinari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MatsuuraYuki
en-aut-sei=Matsuura
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakashimaChieko
en-aut-sei=Takashima
en-aut-mei=Chieko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=Chen-YoshikawaToyofumi Fengshi
en-aut-sei=Chen-Yoshikawa
en-aut-mei=Toyofumi Fengshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=EbiHiromichi
en-aut-sei=Ebi
en-aut-mei=Hiromichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=ImagamaShiro
en-aut-sei=Imagama
en-aut-mei=Shiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=KitohHiroshi
en-aut-sei=Kitoh
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=OhnoKinji
en-aut-sei=Ohno
en-aut-mei=Kinji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=HosonoYasuyuki
en-aut-sei=Hosono
en-aut-mei=Yasuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

affil-num=1
en-affil=Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine
kn-affil=

affil-num=2
en-affil=Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine
kn-affil=

affil-num=3
en-affil=Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine
kn-affil=

affil-num=4
en-affil=Division of Molecular Therapeutics, Aichi Cancer Center Research Institute
kn-affil=

affil-num=5
en-affil=Division of Molecular Therapeutics, Aichi Cancer Center Research Institute
kn-affil=

affil-num=6
en-affil=Division of Molecular Therapeutics, Aichi Cancer Center Research Institute
kn-affil=

affil-num=7
en-affil=Department of Thoracic Surgery, Nagoya University Graduate School of Medicine
kn-affil=

affil-num=8
en-affil=Division of Molecular Therapeutics, Aichi Cancer Center Research Institute
kn-affil=

affil-num=9
en-affil=Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine
kn-affil=

affil-num=10
en-affil=Department of Orthopaedic Surgery, Aichi Childrenfs Health and Medical Center
kn-affil=

affil-num=11
en-affil=Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine
kn-affil=

affil-num=12
en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=FGFR3
kn-keyword=FGFR3
en-keyword=achondroplasia
kn-keyword=achondroplasia
en-keyword=meclozine
kn-keyword=meclozine
en-keyword=zebrafish
kn-keyword=zebrafish
en-keyword=bone
kn-keyword=bone
END