Okayama University Medical SchoolActa Medica Okayama0386-300X7512021Local Control of Squamous Cell Carcinoma of the Cervix Treated with CT-based Three-dimensional Image-Guided Brachytherapy with or without Central Shielding7985ENKotaroYoshioDepartment of Radiology, Kagawa Prefectural Central HospitalHisakoNagasakaDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalKentoHisazumiDepartment of Radiology, Kagawa Prefectural Central HospitalHiroOkawaDepartment of Radiology, Kagawa Prefectural Central HospitalNobuhisaTajiriDepartment of Radiology, Kagawa Prefectural Central HospitalTsuyokiShiodeDepartment of Radiology, Kagawa Prefectural Central HospitalShiroAkakiDepartment of Radiology, Kagawa Prefectural Central HospitalSusumuKanazawaDepartment of Radiology, Okayama University HospitalTomohiroMitomaDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalYuriYanoDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalEmikoKobayashiDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalIkuyoHoriguchiDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalMasayoTakataDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalAtsushiHongoDepartment of Obstetrics and Gynecology 2, Kawasaki Medical School, General medical CenterMasaruYonezawaDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalYoshieNakanishiDepartment of Obstetrics and Gynecology, Kagawa Prefectural Central HospitalOriginal Article10.18926/AMO/61438The purposes of this retrospective study were to analyze local control of squamous cell carcinoma of the cervix treated with computed tomography (CT)-based image-guided brachytherapy (IGBT), as well as the factors affecting local control. A total of 39 patients were analyzed. The prescribed dose to the pelvis was 45-50 Gy with or without central shielding (CS). IGBT was delivered in 1-5 fractions. The total dose for high-risk clinical target volume (HR-CTV) was calculated as the biologically equivalent dose in 2-Gy fractions. The median follow-up period was 29.3 months. The 2-year overall survival and local control rates were 97% and 91%, respectively. In univariate analysis, the dose covering 90% of the HR-CTV (D90) and tumor size were found to be significant factors for local control. The cutoff values of tumor size and D90 for local control were 4.3 cm (area under the curve [AUC] 0.75) and 67.7 Gy (AUC 0.84) in the CS group and 5.3 cm (AUC 0.75) and 73.7 Gy (AUC 0.78) in the group without CS, respectively. However, though the local control of CT-based IGBT was favorable, the results suggested that the dose required for tumor control may differ depending on the presence of CS.No potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X6612012The Pretreatment of Maximum Standardized Uptake Values (SUVmax) of the Primary Tumor Is Predictor for Poor Prognosis for Patients with Epithelial Ovarian Cancer5360ENKeiichiroNakamuraAtsushiHongoJunichiKodamaYujiHiramatsuOriginal Article10.18926/AMO/48081The purpose of this study was to evaluate prognostic factors for epithelial ovarian cancer. We found that the pretreatment values of maximum standardized uptake (SUVmax) of the primary tumor by positron emission tomography/computed tomography (PET/CT), tumor marker CA125 and C-reactive protein (CRP) were correlated with clinical characteristics and prognosis for such patients. The clinical parameters and prognoses and their correlations with SUVmax of primary tumor, CA125 and CRP were examined for 51 patients with primary ovarian cancer. The SUVmax of the primary tumor had a statistically significant association with stage (p＝0.010) and histology (p＝0.001). CA125 was significant associated with stage (p＝0.011), histology (p＝0.005) and lymph node metastasis (p＝0.025). CRP was also significantly associated with stage (p＝0.049). Disease-free survival rates of patients exhibiting a high SUVmax, CA125 and CRP were significantly lower than those exhibiting a low SUVmax, CA125 and CRP levels (p＝0.008, 0.034, and 0.037, respectively). Furthermore, overall survival rates of patients exhibiting a high SUVmax were significantly lower than those exhibiting a low SUVmax (p＝0.049).The high SUVmax of primary tumor is an important factor for identifying ovarian cancer patients with a predictor for poor prognosis.No potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X5612002Neoadjuvant treatment with docetaxel and the effects of irradiation for human ovarian adenocarcinoma and cervical squamous cell carcinoma in vitro.1318ENShinakoArakiYasunariMiyagiKunihiroKawanishiJunkoYamamotoAtsushiHongoJunichiKodamaMitsuoYoshinouchiTakafumiKudoArticle10.18926/AMO/31723<p>The in vitro radiosensitizing effects of docetaxel have been reported, but the DNA damage caused by the irradiation after docetaxel exposure has not been investigated. In this study, the authors attempted to evaluate the radiosensitizing effects in terms of cell survival and DNA single-strand breaks in a human ovarian adenocarcinoma cell line (known as line BG-1) and a human cervical squamous cell carcinoma cell line (known as line SiHa). The cell lines were exposed to various concentrations of docetaxel (from 2.27 x 10(-3) to 2.27 microg/ml) to investigate the cytocidal effects by colony-formation assay. DNA single-strand breaks after exposure to 2.27 microg/ml of docetaxel for 30 min or 100 min were measured by the alkaline-elution assay. The remarkable cytotoxicity of docetaxel followed by irradiation was observed when concentrations were greater than 2.27 x 10(-2) microg/ml in both cell lines. The combination of docetaxel and irradiation appears to be supraadditive. The DNA single-strand breaks induced by the irradiation were enhanced in both cell lines (BG-1; P < 0.01, SiHa; P < 0.05). The synergistic cytocidal effect cannot be explained quantitatively only by the single-strand breaks.
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No potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X6242008Immunohistochemical Evaluation of Insulin-like Growth Factor I Receptor Status in Cervical Cancer Specimens251259ENHiroyukiKuramotoAtsushiHongoYi-xuanLiuYojiroOjimaKeiichiroNakamuraNorikoSekiJunichiKodamaYujiHiramatsuOriginal Article10.18926/AMO/30944<p>The insulin-like growth factor I receptor (IGF-IR) is exceptionally overexpressed in many cervicalcancer-derived cell lines. It is postulated that a decrease of p53 protein levels due to human papillomavirus (HPV) infection may contribute to the up-regulation of IGF-IR expression in cervical cancer cells because transcription of IGF-IR is strictly down-regulated by p53. To evaluate this fact in clinical cervical cancer specimens, we checked the expression levels and activated status of IGF-IR by immunohistochemistry. Formalin-fixed and paraffin-embedded specimens obtained by conization or hysterectomy were stained with anti-IGF-IR and with an antibody recognizing phosphorylated tyrosine at its c-terminus. The expression levels of IGF-IR were significantly high in cervical intraepithelial neoplasia (CIN) III and invasive cancer specimens. Phosphorylation of IGF-IR was promoted in all CIN and invasive cancer specimens, and its intensity was related to the promotion of lesions. Interestingly, IGF-IR overexpression was missing in the basal layer of CIN I and II lesions, whereas it was evenly distributed in CIN III and invasive cancer lesions. This IGF-IR overexpression pattern may be utilized in the diagnosis of HPV infection status in CIN lesions.</p>No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155811712005腫瘍細胞におけるIGF-Iレセプターの役割と婦人科癌分子標的治療への応用2733ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama1994A comparison of in vitro platinum-DNA adduct formation between cisplatin and carboplatinENNo potential conflict of interest relevant to this article was reported.