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ID 30790
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Author
Nanba, Yoshifumi
Nishida, Keiichiro Kaken ID publons researchmap
Yoshikawa, Takeshi
Sato, Toru
Inoue, Hajime
Kuboki, Yoshinori
Abstract

The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.

Keywords
chondrocalcinosis
osteoarthritis
osteonection
Mankin's histologic-histochemical grading
calcification
Amo Type
Article
Publication Title
Acta Medica Okayama
Published Date
1997-10
Volume
volume51
Issue
issue5
Publisher
Okayama University Medical School
Start Page
239
End Page
243
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
English
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT