Cytosolic acidification and oxidation are the toxic mechanisms of SO2 in Arabidopsis guard cells

SO2/H2SO3 can damage plants. However, its toxic mechanism has still been controversial. Two models have been proposed, cytosolic acidification model and cellular oxidation model. Here, we assessed the toxic mechanism of H2SO3 in three cell types of Arabidopsis thaliana, mesophyll cells, guard cells (GCs), and petal cells. The sensitivity of GCs of Chloride channel a (CLCa)-knockout mutants to H2SO3 was significantly lower than those of wildtype plants. Expression of other CLC genes in mesophyll cells and petal cells were different from GCs. Treatment with antioxidant, disodium 4,5-dihydroxy-1,3-benzenedisulfonate (tiron), increased the median lethal concentration (LC50) of H2SO3 in GCs indicating the involvement of cellular oxidation, while the effect was negligible in mesophyll cells and petal cells. These results indicate that there are two toxic mechanisms of SO2 to Arabidopsis cells: cytosolic acidification and cellular oxidation, and the toxic mechanism may vary among cell types.

This is a pre-copyedited, author-produced version of an article accepted for publication in [Bioscience, Biotechnology, and Biochemistry] following peer review. The version of record [Mahdi Mozhgani, Lia Ooi, Christelle Espagne, Sophie Filleur, Izumi C Mori, Cytosolic acidification and oxidation are the toxic mechanisms of SO2 in Arabidopsis guard cells, Bioscience, Biotechnology, and Biochemistry, 2024;, zbae092, https://doi.org/10.1093/bbb/zbae092] is available online at: https://doi.org/10.1093/bbb/zbae092.

This fulltext file will be available in Jul. 2025.