| JaLCDOI | 10.18926/AMO/31301 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Yano, Akemi| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo| |
| Abstract | We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis. |
| Keywords | DNA polymorphism haptoglobin polymerase chain reaction allele-specific amplification personal identification |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 1998-08 |
| Volume | volume52 |
| Issue | issue4 |
| Publisher | Okayama University Medical School |
| Start Page | 173 |
| End Page | 181 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 9781267 |
| Web of Science KeyUT | 000075623600001 |
| JaLCDOI | 10.18926/AMO/30747 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Redsch, Oliver| Miyaishi, Satoru| Heinemann, Axel| Fiedler, Georg| Puschel, Klaus| Yamamoto, Hideki| Ishizu, Hideo| |
| Abstract | The authors designed a questionnaire to investigate the differences in German and Japanese general practitioners? (GP) awareness of suicide and attitudes toward patients with suicidal ideation in their respective societies. The purpose of this study was to obtain insights leading to a better means of suicide prevention in primary care in Japan. The background for conducting the study was declining suicides in the past 20 years and the lower suicide rate in Germany compared with the present situation in Japan, where the number of suicides has in recent years continued to exceed 30,000, resulting in a suicide rate approximately 2 times higher than that in Germany. The questionnaire was randomly mailed to GPs in Okayama-Prefecture (western Japan) and Hamburg-State (northern Germany) and was collected in the same way. The patterns of answers were compared between the 2 countries, and the differences were statistically analyzed. Japanese GPs seem to have a lower will to prevent suicide in daily practice compared to German GPs and a great lack of knowledge about treatment of suicidal patients. These observations suggest that improving GPs? interest in the problem of suicide and providing training programs for the treatment of patients with suicidal intentions might be a means of achieving better suicide prevention in Japan. |
| Keywords | suicide prevention general practitioner Japan Germany |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-06 |
| Volume | volume60 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 159 |
| End Page | 165 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 16838044 |
| Web of Science KeyUT | 000238503600003 |
| Author | Nanikawa, Ryo| Hashimoto, Yoshiaki| Moriya, Fumio| Miyaishi, Satoru| Nakai, Miyoko| Yang, Xu| Guo, Chun Gang| |
|---|---|
| Published Date | 1990-02 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume102 |
| Issue | issue1-2 |
| Content Type | Journal Article |
| Author | Miyaishi, Satoru| Kitao, Takashi| Moriya, Fumio| Yamamoto, Yuji| Ishizu, Hideo| Ishii, Hiroyuki| |
|---|---|
| Published Date | 1991-10 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume103 |
| Issue | issue9-10 |
| Content Type | Journal Article |
| Author | Ishizu, Hideo| Miyaishi, Satoru| Yamamoto, Yuji| Takata, Shingo| |
|---|---|
| Published Date | 1993-10 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume105 |
| Issue | issue9-10 |
| Content Type | Journal Article |
| Author | Ishikawa, Takaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Inagaki, Sachiyo| Okamura, Michihiko| Ishizu, Hideo| |
|---|---|
| Published Date | 2001-12-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume113 |
| Issue | issue3 |
| Content Type | Journal Article |
| Author | Ishikawa, Takaki| Miyaishi, Satoru| Doi, Yusuke| Takata, Tomoyo| Imabayashi, Kiyomi| Inagaki, Sachiyo| Yoshitome, Kei| Yamamoto, Yuji| Ishizu, Hideo| |
|---|---|
| Published Date | 2003-01-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume114 |
| Issue | issue3 |
| Content Type | Journal Article |
| Author | Ishikawa, Takaki| Furusato, Masakuni| Yoshitome, Kei| Miyaishi, Satoru| Ishizu, Hideo| |
|---|---|
| Published Date | 2005-05-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume115 |
| Issue | issue1 |
| Content Type | Journal Article |
| Author | Miyaishi, Satoru| |
|---|---|
| Published Date | 2008-05-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume120 |
| Issue | issue1 |
| Content Type | Journal Article |
| Author | 宮石 智| |
|---|---|
| Published Date | 1991-03-28 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
