start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue= article-no= start-page=102895 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2026 dt-pub=202607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Factors that delay discovery in cases of death at home in Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=In Japan, solitary deaths have become a social issue, with an increasing number of cases in which the body is discovered long after death. This study aimed to clarify the factors underlying such delays. Cases of death at home were extracted from forensic autopsies. In each case, we collected information about the deceased and their living conditions. The postmortem interval until finding (PMI-f) was calculated by measuring the time between death and discovery. We classified the cases into long PMI-f (LPMI-f; having PMI-f of ≥3 days) and short PMI-f (SPMI-f; having PMI-f of <3 days), and examined the factors that lead to LPMI-f. The characteristics of the group living alone with a PMI-f of <24 h and living with family or acquaintances with an LPMI-f were also analyzed. Among the 420 cases included, 244 (58.1%) were in the LPMI-f group. The LPMI-f group had higher number of males; older individuals; those living alone; those found inside their homes; single, retired, or non-employed individuals; those without long-term care certification; and those with drinking habits. Forty-seven individuals lived alone and had PMI-f of <24 h. Regular visits from relatives living separately led to early detection after death. In the LPMI-f group, 56 individuals lived with family or acquaintances. The absence of regular visits can result in delayed discovery after death; however, the time between death and discovery could be shortened by implementing systems such as allowing neighbors to check the person's safety by noticing uncollected mail. en-copyright= kn-copyright= en-aut-name=YamasakiYukie en-aut-sei=Yamasaki en-aut-mei=Yukie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TamiyaNanako en-aut-sei=Tamiya en-aut-mei=Nanako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoHideki en-aut-sei=Yamamoto en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiuraMasanobu en-aut-sei=Miura en-aut-mei=Masanobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaniguchiKaori en-aut-sei=Taniguchi en-aut-mei=Kaori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KobayashiChie en-aut-sei=Kobayashi en-aut-mei=Chie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MotomuraMasafumi en-aut-sei=Motomura en-aut-mei=Masafumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=Himemiya-HakuchoAyako en-aut-sei=Himemiya-Hakucho en-aut-mei=Ayako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Health Services Research, Faculty of Medicine, University of Tsukuba kn-affil= affil-num=3 en-affil=Department of Environmental Health, Faculty of Pharma-Science, Teikyo University kn-affil= affil-num=4 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Faculty of Interdisciplinary Science and Engineering in Health Systems, Okayama University kn-affil= affil-num=8 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Solitary deaths kn-keyword=Solitary deaths en-keyword=Social isolation kn-keyword=Social isolation en-keyword=Postmortem interval kn-keyword=Postmortem interval en-keyword=Decomposition kn-keyword=Decomposition END start-ver=1.4 cd-journal=joma no-vol=27 cd-vols= no-issue=5 article-no= start-page=2113 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2026 dt-pub=20260224 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fgf10 Gene Dosage from a Single Allele Is Insufficient for Forming Multilayered Epithelial Cells in the Murine Lacrimal Gland en-subtitle= kn-subtitle= en-abstract= kn-abstract=Mutations in the fibroblast growth factor 10 (FGF10) gene in humans cause aplasia of the lacrimal and salivary glands (ALSG). In patients with ALSG, heterozygous loss-of-function mutations are found, and FGF10 haploinsufficiency results in the absence of these secretory organs. Lacrimal glands (LGs) are formed through epithelial thickening, budding, and branching morphogenesis. To compare the variable phenotypes of the Fgf10+/− Harderian glands (HGs) previously reported, we examined the development of LGs in wild-type (WT), Fgf10+/−, and Fgf10-null mice. Pax6 immunostaining was performed to visualize the LG primordia from embryonic day 15.5 (E15.5) onwards. In situ hybridization of the genes encoding the epithelial receptor of FGF10, FGFR2b, and its other ligands was performed to determine their potential involvement in LG development. LG primordia were not observed in Fgf10+/− mice bilaterally at E16.5 or later stages. At E15.5, budding from the developing conjunctival epithelium (CE) was observed in a small fraction of the Fgf10+/− LG primordia. In contrast, the Fgf10-null CE failed to promote budding. Among Fgf1, Fgf3, Fgf7, Fgf10, and Fgf22, Fgf10 was expressed in the mesenchyme surrounding developing LG epithelial cells, whereas Fgf1 was expressed in the LG epithelium of WT mice. Fgf7 was initially expressed in the mesenchyme surrounding the nascent LG epithelium, but its expression subsequently became diffused. Thus, we conclude that among the FGFR2b ligands, initial LG formation is dependent on the mesenchymal factors FGF10 and FGF7, and FGF1 is likely to function as an epithelial factor in the LG primordia. A single allele of Fgf10 was found to be insufficient to support the budding process during LG morphogenesis. en-copyright= kn-copyright= en-aut-name=IkedaShiori en-aut-sei=Ikeda en-aut-mei=Shiori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SatoKeita en-aut-sei=Sato en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TajikaYuki en-aut-sei=Tajika en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujitaHirofumi en-aut-sei=Fujita en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=BandoTetsuya en-aut-sei=Bando en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NohnoTsutomu en-aut-sei=Nohno en-aut-mei=Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhuchiHideyo en-aut-sei=Ohuchi en-aut-mei=Hideyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Department of Radiological Technology, Gumma Prefectural College of Health Sciences kn-affil= affil-num=4 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=5 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=6 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=7 en-affil=Department of Legal Medicine, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=8 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=fibroblast growth factor kn-keyword=fibroblast growth factor en-keyword=Fgf10 kn-keyword=Fgf10 en-keyword=Fgf1 kn-keyword=Fgf1 en-keyword=Fgf3 kn-keyword=Fgf3 en-keyword=Fgf7 kn-keyword=Fgf7 en-keyword=Fgf22 kn-keyword=Fgf22 en-keyword=Fgfr2b kn-keyword=Fgfr2b en-keyword=mouse kn-keyword=mouse en-keyword=lacrimal gland kn-keyword=lacrimal gland en-keyword=development kn-keyword=development END start-ver=1.4 cd-journal=joma no-vol=136 cd-vols= no-issue=3 article-no= start-page=141 end-page=141 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20241202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The 108th Annual Meeting of the Japanese Society of Legal Medicine kn-title=第108次日本法医学会学術全国集会開催報告 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Legal Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学学術研究院医歯薬学域　法医学 END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue=2 article-no= start-page=16 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240603 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse en-subtitle= kn-subtitle= en-abstract= kn-abstract=The mouse Harderian gland (HG) is a secretory gland that covers the posterior portion of the eyeball, opening at the base of the nictitating membrane. The HG serves to protect the eye surface from infection with its secretions. Mice open their eyelids at about 2 weeks of age, and the development of the HG primordium mechanically opens the eye by pushing the eyeball from its rear. Therefore, when HG formation is disturbed, the eye exhibits enophthalmos (the slit-eye phenotype), and a line of Fgf10(+/-) heterozygous loss-of-function mice exhibits slit-eye due to the HG atrophy. However, it has not been clarified how and when HGs degenerate and atrophy in Fgf10(+/-) mice. In this study, we observed the HGs in embryonic (E13.5 to E19), postnatal (P0.5 to P18) and 74-week-old Fgf10(+/-) mice. We found that more than half of the Fgf10(+/-) mice had markedly degenerated HGs, often unilaterally. The degenerated HG tissue had a melanized appearance and was replaced by connective tissue, which was observed by P10. The development of HGs was delayed or disrupted in the similar proportion of Fgf10(+/-) embryos, as revealed via histology and the loss of HG-marker expression. In situ hybridization showed Fgf10 expression was observed in the Harderian mesenchyme in wild-type as well as in the HG-lacking heterozygote at E19. These results show that the Fgf10 haploinsufficiency causes delayed or defective HG development, often unilaterally from the unexpectedly early neonatal period. en-copyright= kn-copyright= en-aut-name=IkedaShiori en-aut-sei=Ikeda en-aut-mei=Shiori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SatoKeita en-aut-sei=Sato en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujitaHirofumi en-aut-sei=Fujita en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Ono-MinagiHitomi en-aut-sei=Ono-Minagi en-aut-mei=Hitomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NohnoTsutomu en-aut-sei=Nohno en-aut-mei=Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OhuchiHideyo en-aut-sei=Ohuchi en-aut-mei=Hideyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Cytology and Histology, Medical School, Okayama University kn-affil= affil-num=2 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=Department of Cytology and Histology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=5 en-affil=Department of Legal Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=6 en-affil=Department of Cytology and Histology, Medical School, Okayama University kn-affil= affil-num=7 en-affil=Department of Cytology and Histology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=Harderian gland kn-keyword=Harderian gland en-keyword=Fgf10 kn-keyword=Fgf10 en-keyword=haploinsufficiency kn-keyword=haploinsufficiency en-keyword=mouse kn-keyword=mouse END start-ver=1.4 cd-journal=joma no-vol=134 cd-vols= no-issue=2 article-no= start-page=108 end-page=114 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The role of legal medicine in mass disaster : A report on the activity of the Department of Legal Medicine of Okayama University for the postmortem examination following the Great East Japan Earthquake kn-title=大規模災害に伴う死体検案時の法医学の役割―東日本大震災における岡山大学法医学分野の死体検案支援活動記録と考察 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MiuraMasanobu en-aut-sei=Miura en-aut-mei=Masanobu kn-aut-name=三浦雅布 kn-aut-sei=三浦 kn-aut-mei=雅布 aut-affil-num=1 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=2 ORCID= en-aut-name=TaniguchiKaori en-aut-sei=Taniguchi en-aut-mei=Kaori kn-aut-name=谷口香 kn-aut-sei=谷口 kn-aut-mei=香 aut-affil-num=3 ORCID= en-aut-name=YamasakiYukie en-aut-sei=Yamasaki en-aut-mei=Yukie kn-aut-name=山﨑雪恵 kn-aut-sei=山﨑 kn-aut-mei=雪恵 aut-affil-num=4 ORCID= en-aut-name=KobayashiChie en-aut-sei=Kobayashi en-aut-mei=Chie kn-aut-name=小林智瑛 kn-aut-sei=小林 kn-aut-mei=智瑛 aut-affil-num=5 ORCID= en-aut-name=TakeiSella en-aut-sei=Takei en-aut-mei=Sella kn-aut-name=竹居セラ kn-aut-sei=竹居 kn-aut-mei=セラ aut-affil-num=6 ORCID= en-aut-name=AokiYasuhiro en-aut-sei=Aoki en-aut-mei=Yasuhiro kn-aut-name=青木康博 kn-aut-sei=青木 kn-aut-mei=康博 aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　法医学 affil-num=2 en-affil=Department of Legal Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学学術研究院医歯薬学域　法医学 affil-num=3 en-affil=Department of Legal Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学学術研究院医歯薬学域　法医学 affil-num=4 en-affil=Department of Legal Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学学術研究院医歯薬学域　法医学 affil-num=5 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　法医学 affil-num=6 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　法医学 affil-num=7 en-affil=Department of Forensic Medicine, Nagoya City University Graduate School of Medical Sciences kn-affil=名古屋市立大学大学院医学研究科　法医学 END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=4 article-no= start-page=45 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20221029 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium en-subtitle= kn-subtitle= en-abstract= kn-abstract=The first event of differentiation and morphogenesis in the optic vesicle (OV) is specification of the neural retina (NR) and retinal pigment epithelium (RPE), separating the inner and outer layers of the optic cup, respectively. Here, we focus on a basic helix-loop-helix gene, BHLHE40, which has been shown to be expressed by the developing RPE in mice and zebrafish. Firstly, we examined the expression pattern of BHLHE40 in the developing chicken eye primordia by in situ hybridization. Secondly, BHLHE40 overexpression was performed with in ovo electroporation and its effects on optic cup morphology and expression of NR and RPE marker genes were examined. Thirdly, we examined the expression pattern of BHLHE40 in LHX1-overexpressed optic cup. BHLHE40 expression emerged in a subset of cells of the OV at Hamburger and Hamilton stage 14 and became confined to the outer layer of the OV and the ciliary marginal zone of the retina by stage 17. BHLHE40 overexpression in the prospective NR resulted in ectopic induction of OTX2 and repression of VSX2. Conversely, BHLHE40 was repressed in the second NR after LHX1 overexpression. These results suggest that emergence of BHLHE40 expression in the OV is involved in initial RPE specification and that BHLHE40 plays a role in separation of the early OV domains by maintaining OTX2 expression and antagonizing an NR developmental program. en-copyright= kn-copyright= en-aut-name=KinuhataToshiki en-aut-sei=Kinuhata en-aut-mei=Toshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SatoKeita en-aut-sei=Sato en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=BandoTetsuya en-aut-sei=Bando en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MitoTaro en-aut-sei=Mito en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NohnoTsutomu en-aut-sei=Nohno en-aut-mei=Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OhuchiHideyo en-aut-sei=Ohuchi en-aut-mei=Hideyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Bio-Innovation Research Center, Tokushima University kn-affil= affil-num=5 en-affil=Department of Legal Medicine, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Cytology and Histology, Okayama University Medical School kn-affil= affil-num=7 en-affil=Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=basic helix-loop-helix e40 kn-keyword=basic helix-loop-helix e40 en-keyword=BHLHE40 kn-keyword=BHLHE40 en-keyword=LIM homeobox 1 kn-keyword=LIM homeobox 1 en-keyword=LHX1 kn-keyword=LHX1 en-keyword=chicken kn-keyword=chicken en-keyword=optic vesicle kn-keyword=optic vesicle en-keyword=retinal pigment epithelium kn-keyword=retinal pigment epithelium en-keyword=RPE kn-keyword=RPE en-keyword=neural retina kn-keyword=neural retina END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue=10 article-no= start-page=e0240333 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20201015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung en-subtitle= kn-subtitle= en-abstract= kn-abstract=CRISPR/Cas9-mediated gene editing often generates founder generation (F0) mice that exhibit somatic mosaicism in the targeted gene(s). It has been known thatFibroblast growth factor 10(Fgf10)-null mice exhibit limbless and lungless phenotypes, while intermediate limb phenotypes (variable defective limbs) are observed in theFgf10-CRISPR F0 mice. However, how the lung phenotype in theFgf10-mosaic mutants is related to the limb phenotype and genotype has not been investigated. In this study, we examined variable lung phenotypes in theFgf10-targeted F0 mice to determine if the lung phenotype was correlated with percentage of functionalFgf10genotypes. Firstly, according to a previous report,Fgf10-CRISPR F0 embryos on embryonic day 16.5 (E16.5) were classified into three types: type I, no limb; type II, limb defect; and type III, normal limbs. Cartilage and bone staining showed that limb truncations were observed in the girdle, (type I), stylopodial, or zeugopodial region (type II). Deep sequencing of theFgf10-mutant genomes revealed that the mean proportion of codons that encode putative functional FGF10 was 8.3 +/- 6.2% in type I, 25.3 +/- 2.7% in type II, and 54.3 +/- 9.5% in type III (mean +/- standard error of the mean) mutants at E16.5. Histological studies showed that almost all lung lobes were absent in type I embryos. The accessory lung lobe was often absent in type II embryos with other lobes dysplastic. All lung lobes formed in type III embryos. The number of terminal tubules was significantly lower in type I and II embryos, but unchanged in type III embryos. To identify alveolar type 2 epithelial (AECII) cells, known to be reduced in theFgf10-heterozygous mutant, immunostaining using anti-surfactant protein C (SPC) antibody was performed: In the E18.5 lungs, the number of AECII was correlated to the percentage of functionalFgf10genotypes. These data suggest theFgf10gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung. Since dysfunction of AECII cells has been implicated in the pathogenesis of parenchymal lung diseases, theFgf10-CRISPR F0 mouse would present an ideal experimental system to explore it. en-copyright= kn-copyright= en-aut-name=HabutaMunenori en-aut-sei=Habuta en-aut-mei=Munenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YasueAkihiro en-aut-sei=Yasue en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuzukiKen-Ichi T. en-aut-sei=Suzuki en-aut-mei=Ken-Ichi T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujitaHirofumi en-aut-sei=Fujita en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SatoKeita en-aut-sei=Sato en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KonoHitomi en-aut-sei=Kono en-aut-mei=Hitomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakayamaAyuko en-aut-sei=Takayama en-aut-mei=Ayuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=BandoTetsuya en-aut-sei=Bando en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OyadomariSeiichi en-aut-sei=Oyadomari en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=TanakaEiji en-aut-sei=Tanaka en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=OhuchiHideyo en-aut-sei=Ohuchi en-aut-mei=Hideyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School kn-affil= affil-num=3 en-affil=Department of Mathematical and Life Sciences, Hiroshima University kn-affil= affil-num=4 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Center for the Development of New Model Organisms, National Institute for Basic Biology kn-affil= affil-num=8 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Division of Molecular Biology, Institute of Advanced Medical Sciences, Tokushima University kn-affil= affil-num=11 en-affil=Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School kn-affil= affil-num=12 en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=5 article-no= start-page=299 end-page=306 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201110 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Quantitative Analysis of DNA Degradation in the Dead Body en-subtitle= kn-subtitle= en-abstract= kn-abstract=Postmortem degradation of DNA was quantitatively estimated. Brain, liver, kidney and muscle samples were obtained from sacrificed rats left at 20℃ or 4℃. The quantity of DNA was measured by real-time PCR using a primer set for a sequence in the Rsrc 1 gene. When the quantity of amplified DNA using 10ng Human Genomic DNA was defined as 100 RFU, the quantities in the brain, liver, kidney and skeletal muscle (each 2μg of dry weight) on the day of sacrifice were 253±11, 338±22, 556±14 and 531±12 Relative Fluorescence Units (RFU), respectively (mean±S.E., n＝5). The quantity of amplified DNA decreased to below 10 RFU in 1-3 weeks in the liver, kidney and skeletal muscle at 20℃, while that in the brain was more than 10 RFU for six weeks, demonstrating the usefulness of the brain as a sample for DNA analysis of decaying corpses. It was suggested that quantifying the amplified DNA in the brain at 20℃ and in the liver at 4℃ as well as the ratio of the quantity of amplified DNA in the liver to the brain at 4℃ might be useful for diagnosing time of death. This study provides the first quantitative analysis of the postmortem progress of DNA degradation in the corpse. en-copyright= kn-copyright= en-aut-name=ItaniMiki en-aut-sei=Itani en-aut-mei=Miki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=DoiYusuke en-aut-sei=Doi en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=DNA degradation kn-keyword=DNA degradation en-keyword=postmortem interval kn-keyword=postmortem interval en-keyword=personal identification kn-keyword=personal identification END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=4 article-no= start-page=225 end-page=230 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Postmortem Changes in Myoglobin Content in Organs en-subtitle= kn-subtitle= en-abstract= kn-abstract=Postmortem changes in myoglobin concentrations in blood and organs were investigated using an enzyme immunoassay by animal experiments in combination with immunohistochemical staining of human cases. Blood myoglobin concentrations were found to increase drastically within a very short time after death. Those in striated muscle, however, did not change by day 14 postmortem. Myoglobin content in the liver and kidney increased slightly by day 5 postmortem, and more obviously by day 7 or later. However, almost no change was observed by day 5 in the kidney when the renal artery and vein had been ligated just after death. In the thyroid gland and the lung, the myoglobin content markedly increased by day 7 postmortem, with the logarithmical values rising nearly linearly as the time after death passed. In the thyroid gland, concentrations reached the level of the striated muscle. The mechanisms of postmortem myoglobin increase in organs are thought to be direct diffusion from the striated muscle and/or distribution through the blood. To estimate the postmortem interval, the determination of myoglobin content in the thyroid gland or the lung appears to be useful. en-copyright= kn-copyright= en-aut-name=MiuraMasanobu en-aut-sei=Miura en-aut-mei=Masanobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakaToru en-aut-sei=Naka en-aut-mei=Toru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=myoglobin kn-keyword=myoglobin en-keyword=postmortem diffusion kn-keyword=postmortem diffusion en-keyword=postmortem distribution kn-keyword=postmortem distribution en-keyword=postmortem interval kn-keyword=postmortem interval END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=2 article-no= start-page=113 end-page=122 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A New Method for Sex Determination Based on Detection of SRY, STS and Amelogenin Gene Regions with Simultaneous Amplification of Their Homologous Sequences by a Multiplex PCR en-subtitle= kn-subtitle= en-abstract= kn-abstract=We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male:female ratio as low as 1:10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described. en-copyright= kn-copyright= en-aut-name=MorikawaToshio en-aut-sei=Morikawa en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=sex determination kn-keyword=sex determination en-keyword=SRY (sex-determining region Y) kn-keyword=SRY (sex-determining region Y) en-keyword=multiplex PCR kn-keyword=multiplex PCR en-keyword=forensic casework kn-keyword=forensic casework END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=2 article-no= start-page=59 end-page=71 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200304 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Analysis of short tandem repeat (STR) polymorphisms by the powerplex 16 system and capillary electrophoresis: application to forensic practice. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Allele and genotype frequencies for 15 short tandem repeat (STR) polymorphisms--D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA--in a Japanese population were estimated. No deviations of the observed allele frequency from Hardy-Weinberg equilibrium expectations were found for any of the systems studied. Between 2 new pentanucleotide STR loci, Penta E and Penta D, for which there is only limited data regarding the allelic distribution in Japanese, the Penta E locus was found to be highly polymorphic and exhibited a tri- or tetra-modal distribution pattern having allelic peaks with 5, 11, 15 and 20 repeats. The distribution was significantly different from that of the other ethnic groups. Statistical parameters of forensic importance, the power of discrimination (PD), observed and expected heterozygosity values (H), polymorphism information content (PIC), power of discrimination (PD), matching probability (pM), power of exclusion (PE), and typical paternity index (PI), were calculated for the loci. These parameters indicated the usefulness of the loci in forensic personal identification and paternity testing among Japanese. The systems Penta E, FGA, D18S51 and D8S1179 were the most informative. This method was successfully applied to forensic personal identification and paternity testing among Japanese, thereby confirming its efficacy for forensic practice.

en-copyright= kn-copyright= en-aut-name=OkamotoOsamu en-aut-sei=Okamoto en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InagakiSachiyo en-aut-sei=Inagaki en-aut-mei=Sachiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ishikawaTakaki en-aut-sei=ishikawa en-aut-mei=Takaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ImabayashiKiyomi en-aut-sei=Imabayashi en-aut-mei=Kiyomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=population data kn-keyword=population data en-keyword=DNA typing kn-keyword=DNA typing en-keyword=short tandem repests kn-keyword=short tandem repests en-keyword=personal identification kn-keyword=personal identification en-keyword=paternity testing kn-keyword=paternity testing END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=2 article-no= start-page=53 end-page=58 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200304 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Increase of S-100 protein-positive stellate cells in the anterior pituitary of chronic alcoholic patients with fatty liver or fatty cirrhosis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Healthy subjects 40 years old were used as controls in a study of stellate cells (S-100 protein-containing cells, or S-100 cells) in subjects with chronic alcoholism and fatty liver or fatty cirrhosis. S-100 cells were sparsely found in the adenohypophysis of control subjects, and these cells sometimes formed small clusters. However, in chronic alcoholics with fatty liver or fatty cirrhosis, the number of stellate cells in the anterior pituitary tended to be 17 times higher than it was in the control group. No increase in the number of S-100 positive cells that constitute the large and small follicles in the intermediate pituitary. The physiological function of the S-100 protein has not yet been identified. The fact that an increase in prolactin-secreting and growth hormone-secreting cells, as well as a decrease in gonadotrophs were observed in the hypophysis of alcoholics suggests that the function of stellate cells may be closely related to these phenomena. Our results also imply that the stellate cells found in the anterior and intermediate pituitary differ in function although they both produce S-100 proteins.

en-copyright= kn-copyright= en-aut-name=IshikawaTakaki en-aut-sei=Ishikawa en-aut-mei=Takaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TachibanaToshiaki en-aut-sei=Tachibana en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IshikawaHiroshi en-aut-sei=Ishikawa en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Jikei University affil-num=3 en-affil= kn-affil=Jikei University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=S-100 protein kn-keyword=S-100 protein en-keyword=pituitary kn-keyword=pituitary en-keyword=alcoholism kn-keyword=alcoholism END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=2 article-no= start-page=83 end-page=89 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200304 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Role of adenohypophyseal mixed cell-follicles in age estimation. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In this study we used paraffin-embedded human pituitary obtained from 248 autopsy cases and identified mixed cell follicles by the immunohistochemical method. We examined the number and size of the mixed cell follicles, and the ratio of each component cell of these follicles, in the anterior pituitary at various age groups. The number of follicles increased with age, and the size of the follicles also tended to enlarge with age. Statistical analysis showed that a high correlation existed between age and the number or the size of the mixed cell-follicles formed by various adenohypophyseal cells. In addition, when the proportions of the different cell types that formed the follicles were examined, sex differences were observed with aging for the GH cells, the PRL cells, and the gonadotroph (GTH) cells, while no changes were observed with aging in both men and women for the ACTH cells and TSH cells. These results indicate that the number, size, and ratio of each component cell of follicles in the anterior pituitary are adequately applicable for the purpose of age estimation in routine forensic medicine.

en-copyright= kn-copyright= en-aut-name=IshikawaTakaki en-aut-sei=Ishikawa en-aut-mei=Takaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TachibanaToshiaki en-aut-sei=Tachibana en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Jikei University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=mixed cell-follicle kn-keyword=mixed cell-follicle en-keyword=human anterior pituitary kn-keyword=human anterior pituitary en-keyword=age estimation kn-keyword=age estimation END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=1 article-no= start-page=21 end-page=32 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Forensic study of sex determination using PCR on teeth samples. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In this study, sex determination using polymerase chain reaction (PCR) on tooth material was evaluated from the viewpoint of forensic medicine. The sensitivity of PCR for detection of the Y chromosome-specific alphoid repeat sequence and the X chromosome-specific alphoid repeat sequence was 0.5 pg of genomic DNA. Sex could be determined by PCR of DNA extracted from the pulp of 16 freshly extracted permanent teeth and dentine including the surface of the pulp cavity of 6 freshly extracted milk teeth. Sex could be determined using the pulp in all 20 teeth (10 male and 10 female) preserved at room temperature for 22 years. For the pulp of teeth stored in sea water, the sex could be determined in all 8 teeth immersed for 1 week and in 5 of 6 teeth immersed for 4 weeks. In the remaining 1 tooth, in which sex determination based on the pulp failed, the sex could be determined correctly when DNA extracted from the tooth hard tissue was examined. For teeth stored in soil, the sex could be determined accurately in all 8 teeth buried for 1 week, 7 of 8 teeth buried for 4 weeks, and in all 6 teeth buried for 8 weeks. When teeth were heated for 30 min, sex determination from the pulp was possible in all teeth heated to 100, 150, and 200 degrees C, and even in some teeth heated to 250 degrees C. When this method was applied to actual forensic cases, the sex of a mummified body estimated to have been discovered half a year to 1 year after death could be determined readily by examination of the dental pulp. In the skeletons of 2 bodies placed under water for approximately 1 year and approximately 11 years and 7 months, pulp tissues had been dissolved and lost, but sex determination was possible using DNA extracted from hard dental tissues. These results indicate that this method is useful in forensic practices for sex determination based on teeth samples.

en-copyright= kn-copyright= en-aut-name=MurakamiHiroki en-aut-sei=Murakami en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YmamamotoYuji en-aut-sei=Ymamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OnoToshiaki en-aut-sei=Ono en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkamotoOsamu en-aut-sei=Okamoto en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShigetaYoshiaki en-aut-sei=Shigeta en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=DoiYusuke en-aut-sei=Doi en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University en-keyword=personal identification kn-keyword=personal identification en-keyword=sex determination kn-keyword=sex determination en-keyword=tooth kn-keyword=tooth en-keyword=deoxyribonucleic acid (DNA). polymerase chain reaction kn-keyword=deoxyribonucleic acid (DNA). polymerase chain reaction END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=3 article-no= start-page=175 end-page=184 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Human identification from forensic materials by amplification of a human-specific sequence in the myoglobin gene. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degrees C for 6 months, and from bloodstains heated at 150 degrees C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.</P>

en-copyright= kn-copyright= en-aut-name=OnoToshiaki en-aut-sei=Ono en-aut-mei=Toshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IshikawaTakaki en-aut-sei=Ishikawa en-aut-mei=Takaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=species identification kn-keyword=species identification en-keyword=myoglobin kn-keyword=myoglobin en-keyword=polymerase chain reaction kn-keyword=polymerase chain reaction END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=5 article-no= start-page=179 end-page=194 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=200510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A new HLA-DRB1 genotyping method using single nucleotide polymorphism (SNP) analysis with multiplex primer extension reactions and its application to mixed samples. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine.

en-copyright= kn-copyright= en-aut-name=ImabayashiKiyomi en-aut-sei=Imabayashi en-aut-mei=Kiyomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InagakiSachiyo en-aut-sei=Inagaki en-aut-mei=Sachiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DoiYusuke en-aut-sei=Doi en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama Prefectural Police Headquarters affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=HLA-DRB1 genotyping kn-keyword=HLA-DRB1 genotyping en-keyword=group specific primer kn-keyword=group specific primer en-keyword=single nucleotide polymorphism kn-keyword=single nucleotide polymorphism en-keyword=multiplex primer extension reactions kn-keyword=multiplex primer extension reactions en-keyword=application to mixed samples kn-keyword=application to mixed samples END start-ver=1.4 cd-journal=joma no-vol=63 cd-vols= no-issue=4 article-no= start-page=177 end-page=186 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=200908 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Factors affecting the choice of suicide method in Okayama: a database analysis from a forensic perspective en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The annual number of suicides in Japan increased sharply in 1998, and since that time it has consistently exceeded 30,000 per year. In this study, we analyze a database of personal and background characteristics of 824 cases (605 men, 219 women) who completed suicide in Okayama Prefecture in 2002 and 2003. The data were obtained with cooperation from the police. Using the methodologies in a previous European study as a model, we classified the suicide methods into 8 categories. To examine the generational and regional differences in the choice of methods, we stratified the sample into 4 age groups (<-24, 2544, 4564, and >-65) and 2 regional groups (Okayama/Kurashiki vs. other areas). Our results on gender differences in 7 of the suicide methods were mostly similar to the European data. However, our data showed a remarkably higher proportionate male-to-female mortality ratio for poisoning by other substances (ICD-10, X65-X69 codes) (1.83, 1.15-2.92). In terms of generational differences in the choice of suicide methods, the Mantel-Haenszel test of homogeneity was significant for most of the categories in our study, suggesting an impact of age on how people commit suicide. There were no remarkable regional differences in our sample. An epidemic curve for suicides via carbon monoxide poisoning using charcoal briquets revealed a trend of time clustering not observed in the other 6 means. The database constructed and used in this study contains richer information than conventional death statistics and is expected to provide helpful knowledge and insights for future epidemiological studies.

en-copyright= kn-copyright= en-aut-name=KamizatoEigo en-aut-sei=Kamizato en-aut-mei=Eigo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IwaseToshihide en-aut-sei=Iwase en-aut-mei=Toshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsudaToshihide en-aut-sei=Tsuda en-aut-mei=Toshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=DoiHiroyuki en-aut-sei=Doi en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Human Ecology, Graduate School of Environmental Science, Okayama University affil-num=6 en-affil= kn-affil=Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=suicide methods kn-keyword=suicide methods en-keyword=gender-specific kn-keyword=gender-specific en-keyword=legal medicine kn-keyword=legal medicine en-keyword=cluster suicide kn-keyword=cluster suicide END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=1 article-no= start-page=53 end-page=55 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=200202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A case of suffocation by an advertising balloon filled with pure helium gas. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We encountered a rare case of suffocation by an advertising balloon filled with pure helium gas. Suffocation caused by inhalation of atmosphere lacking in oxygen is not exceptional, but reports of death by suffocation due to a pure inert gas such as helium are very rare. In this case, the balloon mooring on the ground was enclosed, warning signs were displayed, and it was clear that entering the balloon filled with an atmosphere lacking in oxygen was extremely dangerous and should not be done; the accident did, however, occur. Accidents of this kind may occur in the future unless appropriate education and countermeasures are taken.

en-copyright= kn-copyright= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshikawaTakaki en-aut-sei=Ishikawa en-aut-mei=Takaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InagakiSachiyo en-aut-sei=Inagaki en-aut-mei=Sachiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=asphyxia kn-keyword=asphyxia en-keyword=suffocation kn-keyword=suffocation en-keyword=helium kn-keyword=helium en-keyword=advertising balloon kn-keyword=advertising balloon en-keyword=atmosphere lacking in oxygen kn-keyword=atmosphere lacking in oxygen END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=5 article-no= start-page=229 end-page=236 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=200210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evaluation of a method for typing the microsatellite D12S391 locus using a new primer pair and capillary electrophoresis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We describe a modified method for typing a polymorphic microsatellite D12S391 locus by PCR using a newly designed primer pair. This primer pair produces shorter D12S391 amplified fragments (104-156 bp) than the primer pair originally described by Lareu et al. (209-261 bp). The detection system for the D12S391 locus using the new primer pair and capillary electrophoresis (CE) analysis was evaluated using various forensic samples. The typing results from 70 DNA samples using the new primer pair and the original primer pair were completely identical. One hundred twenty-five amplified fragments from D12S391 alleles were sized correctly within +/- 0.25 bp of the D12S391 allelic ladder. A rare allele, 19.3, previously found only in Caucasians, was found for the first time in a Japanese subject, and it was clearly distinguished from allele 20 by the CE analysis. This detection system was sensitive and could detect D12S391 types from 16 pg of genomic DNA, and from a minor component at a ratio of 1:10 in mixed samples. This system was more useful for the analysis of degraded DNA than was the method using the original primer pair, and could detect D12S391 types from bloodstains that had been stored for 26 years. In addition, the specificity of the method was demonstrated using nonhuman DNA.

en-copyright= kn-copyright= en-aut-name=ShigetaYoshiaki en-aut-sei=Shigeta en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=DoiYusuke en-aut-sei=Doi en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=short tandem repeats kn-keyword=short tandem repeats en-keyword=D12S391 kn-keyword=D12S391 en-keyword=forensic application kn-keyword=forensic application en-keyword=capillary electrophoresis kn-keyword=capillary electrophoresis END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=289 end-page=296 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The products of the second round PCRs were digested with restriction endonucleases for the typing of HLA-DRB1 alleles. By this method, HLA-DRB1 typing was possible from 10 pg of genomic DNA extracted from lymphocytes and from 0.5 microliter of 1,000 times diluted blood without DNA extraction. HLA-DRB1 alleles could be typed from a 2-mm long bloodstained cotton thread prepared from 10 times diluted blood and from a 2-mm thread of whole blood bloodstains stored at room temperature for 2 years. From the mixture of blood of two individuals with different genotypes, DRB1 alleles of the minor component were detected down to 1/1,000 of the major component. This semi-nested PCR-RFLP method is useful for HLA-DRB1 typing from extremely small amounts of DNA and from mixed samples.

en-copyright= kn-copyright= en-aut-name=InoueSeiichi en-aut-sei=Inoue en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkamotoOsamu en-aut-sei=Okamoto en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiHiroki en-aut-sei=Murakami en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IsizuHideo en-aut-sei=Isizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Osaka University en-keyword=polymorphism kn-keyword=polymorphism en-keyword=HLA-DRB1 kn-keyword=HLA-DRB1 en-keyword=polymerase chain reaction kn-keyword=polymerase chain reaction en-keyword=dsmi-nested PCR kn-keyword=dsmi-nested PCR en-keyword=restricton fragment length polymotphism kn-keyword=restricton fragment length polymotphism END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=173 end-page=181 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Haptoglobin genotyping by allele-specific polymerase chain reaction amplification en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.

en-copyright= kn-copyright= en-aut-name=YanoAkemi en-aut-sei=Yano en-aut-mei=Akemi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama Uniiversity en-keyword=DNA polymorphism kn-keyword=DNA polymorphism en-keyword=haptoglobin kn-keyword=haptoglobin en-keyword=polymerase chain reaction kn-keyword=polymerase chain reaction en-keyword=allele-specific amplification kn-keyword=allele-specific amplification en-keyword=personal identification kn-keyword=personal identification END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=3 article-no= start-page=159 end-page=165 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200606 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comparison of German and Japanese general practitioners' awareness of suicide and attitudes toward patients with suicidal ideation. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The authors designed a questionnaire to investigate the differences in German and Japanese general practitioners? (GP) awareness of suicide and attitudes toward patients with suicidal ideation in their respective societies. The purpose of this study was to obtain insights leading to a better means of suicide prevention in primary care in Japan. The background for conducting the study was declining suicides in the past 20 years and the lower suicide rate in Germany compared with the present situation in Japan, where the number of suicides has in recent years continued to exceed 30,000, resulting in a suicide rate approximately 2 times higher than that in Germany. The questionnaire was randomly mailed to GPs in Okayama-Prefecture (western Japan) and Hamburg-State (northern Germany) and was collected in the same way. The patterns of answers were compared between the 2 countries, and the differences were statistically analyzed. Japanese GPs seem to have a lower will to prevent suicide in daily practice compared to German GPs and a great lack of knowledge about treatment of suicidal patients. These observations suggest that improving GPs? interest in the problem of suicide and providing training programs for the treatment of patients with suicidal intentions might be a means of achieving better suicide prevention in Japan. en-copyright= kn-copyright= en-aut-name=RedschOliver en-aut-sei=Redsch en-aut-mei=Oliver kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HeinemannAxel en-aut-sei=Heinemann en-aut-mei=Axel kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FiedlerGeorg en-aut-sei=Fiedler en-aut-mei=Georg kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=PuschelKlaus en-aut-sei=Puschel en-aut-mei=Klaus kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YamamotoHideki en-aut-sei=Yamamoto en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=University Medical Center Hamburg-Eppendorf, Germany affil-num=4 en-affil= kn-affil=University Medical Center Hamburg-Eppendorf affil-num=5 en-affil= kn-affil=University Medical Center Hamburg-Eppendorf affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=suicide prevention kn-keyword=suicide prevention en-keyword=general practitioner kn-keyword=general practitioner en-keyword=Japan kn-keyword=Japan en-keyword=Germany kn-keyword=Germany END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=1-2 article-no= start-page=23 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Medico-legal aspects of traumatic injures-Rrport of 13 autopsy cases- kn-title=損傷の法医学的考察―司法解剖13例について― en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this paper we present 13 autopsy cases on interest in medico-legal traumatology. The cases are as follows : 1)two cases of incomplete cardiac rupture dur to thoracic trauma, 2)rupture of herniated large intestine due to abdominal trauma, 3)large incised wounds on the side of the neck by a small kitchen knife, 4)a homicidal case with similar wounds that accompanied self-inflicted wounds, 5)a homicidal case accompanied by multiple stab wounds on the chest, 6)an accidental death skewered by a slender metallic stick, 7)special fatal shotgun injuries, 8)a homicidal case accompanid by various burns by an electric hair-dryer, iron and ciga-rettes, 9)two murder cases by a metallic baseball bat, 10)a suicidal case due to a fall from a high place, and 11)a severely disrupted victim of a road traffic accident. en-copyright= kn-copyright= en-aut-name=NanikawaRyo en-aut-sei=Nanikawa en-aut-mei=Ryo kn-aut-name=何川凉 kn-aut-sei=何川 kn-aut-mei=凉 aut-affil-num=1 ORCID= en-aut-name=HashimotoYoshiaki en-aut-sei=Hashimoto en-aut-mei=Yoshiaki kn-aut-name=橋本良明 kn-aut-sei=橋本 kn-aut-mei=良明 aut-affil-num=2 ORCID= en-aut-name=MoriyaFumio en-aut-sei=Moriya en-aut-mei=Fumio kn-aut-name=守屋文夫 kn-aut-sei=守屋 kn-aut-mei=文夫 aut-affil-num=3 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=4 ORCID= en-aut-name=NakaiMiyoko en-aut-sei=Nakai en-aut-mei=Miyoko kn-aut-name=中井三代子 kn-aut-sei=中井 kn-aut-mei=三代子 aut-affil-num=5 ORCID= en-aut-name=YangXu en-aut-sei=Yang en-aut-mei=Xu kn-aut-name=徐揚 kn-aut-sei=徐 kn-aut-mei=揚 aut-affil-num=6 ORCID= en-aut-name=GuoChun Gang en-aut-sei=Guo en-aut-mei=Chun Gang kn-aut-name=郭春鋼 kn-aut-sei=郭 kn-aut-mei=春鋼 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部法医学教室 en-keyword=Criminal injuries kn-keyword=Criminal injuries en-keyword=Injuries and utensils kn-keyword=Injuries and utensils en-keyword=Traumatic injuries kn-keyword=Traumatic injuries en-keyword=Medico-legal traumatology kn-keyword=Medico-legal traumatology END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=9-10 article-no= start-page=1103 end-page=1107 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199110 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Blood myoglobin level after trauma and exercise kn-title=外傷事例及び軽度運動負荷時の血中ミオグロビン濃度 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The blood myoglobin level was determined in 18 trauma cases of long bone fracture and the changes in the blood myoglobin level and activities of enzymes in the blood exuded from the muscle tissues after a slight exercise of a 2-km marathon of healthy persons were determined. The blood myoglobin level of the trauma cases increased while there were no changes in the blood myoglobin level and activities of the enzymes in the blood after a 2-km marathon. The blood myoglobin level may be of help to determine whether a man has been injured or not even if he had been exercising at the time of injury. en-copyright= kn-copyright= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=1 ORCID= en-aut-name=KitaoTakashi en-aut-sei=Kitao en-aut-mei=Takashi kn-aut-name=北尾孝司 kn-aut-sei=北尾 kn-aut-mei=孝司 aut-affil-num=2 ORCID= en-aut-name=MoriyaFumio en-aut-sei=Moriya en-aut-mei=Fumio kn-aut-name=守屋文夫 kn-aut-sei=守屋 kn-aut-mei=文夫 aut-affil-num=3 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name=山本雄二 kn-aut-sei=山本 kn-aut-mei=雄二 aut-affil-num=4 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name=石津日出雄 kn-aut-sei=石津 kn-aut-mei=日出雄 aut-affil-num=5 ORCID= en-aut-name=IshiiHiroyuki en-aut-sei=Ishii en-aut-mei=Hiroyuki kn-aut-name=石井啓行 kn-aut-sei=石井 kn-aut-mei=啓行 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=6 en-affil= kn-affil=岡山医学検査センター en-keyword=Injury kn-keyword=Injury en-keyword=Exercise kn-keyword=Exercise en-keyword=Myoglobin kn-keyword=Myoglobin END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=9-10 article-no= start-page=839 end-page=845 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=199310 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=An autopsy case of accidental death from erroneous drip infusion of thrombin kn-title=トロンビン点滴静注による事故死の一剖検例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 63-year-old male died in the hospital from erroneous drip infusion of thrombin, a local hemostatic, after polypectomy for intestinal polyposis. At autopsy, thromboses were observed in the cutaneous veins of the right arm, the right axillary vein and the right subclavian vein, and soft fibrin clots in the superior vena cava, the right atrium, the right ventricle and the pulmonary arteries. Thrombi were also found in the rami of the arteries in both lungs under the microscope. Leucocyte infiltration was recognized in the thrombus, but no organization. This finding shows that the thrombi were fresh. The remainder (150ml) of the drip solution was analyzed for thrombin activity and 8 to 16 units of thrombin activity per ml were demonstrated. From these results, the cause of death was considered pulmonary thrombosis due to intravenous administration of thrombin. en-copyright= kn-copyright= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name=石津日出雄 kn-aut-sei=石津 kn-aut-mei=日出雄 aut-affil-num=1 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=2 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name=山本雄二 kn-aut-sei=山本 kn-aut-mei=雄二 aut-affil-num=3 ORCID= en-aut-name=TakataShingo en-aut-sei=Takata en-aut-mei=Shingo kn-aut-name=高田真吾 kn-aut-sei=高田 kn-aut-mei=真吾 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部法医学教室 en-keyword=トロンビン kn-keyword=トロンビン en-keyword=肺動脈血栓症 kn-keyword=肺動脈血栓症 en-keyword=医療事故 kn-keyword=医療事故 END start-ver=1.4 cd-journal=joma no-vol=113 cd-vols= no-issue=3 article-no= start-page=241 end-page=245 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20011231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Two judicial autopsy cases of sudden unexpected natural death kn-title=外因死の疑いで司法解剖となった内因性急死の2例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Two judicial autopsy cases of sudden unexpected natural death were reported. In both cases it was suspected that the victims had died of a traffic accident or violent injury. It became clear, however, that both the cause of death had been myocardial infarction by the forensic autopsies and that the suspects had not been criminally responsible for the death of the victims. It is expected that sudden unexpected natural death whose cause and manner are unclear at first may occur more frequently following the increase of the number of death from ischemic heart disease in the future. In these circumstances the forensic autopsy of such cases will become more important socially. en-copyright= kn-copyright= en-aut-name=IshikawaTakaki en-aut-sei=Ishikawa en-aut-mei=Takaki kn-aut-name=石川隆紀 kn-aut-sei=石川 kn-aut-mei=隆紀 aut-affil-num=1 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=2 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name=山本雄二 kn-aut-sei=山本 kn-aut-mei=雄二 aut-affil-num=3 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name=吉留敬 kn-aut-sei=吉留 kn-aut-mei=敬 aut-affil-num=4 ORCID= en-aut-name=InagakiSachiyo en-aut-sei=Inagaki en-aut-mei=Sachiyo kn-aut-name=稲垣幸代 kn-aut-sei=稲垣 kn-aut-mei=幸代 aut-affil-num=5 ORCID= en-aut-name=OkamuraMichihiko en-aut-sei=Okamura en-aut-mei=Michihiko kn-aut-name=岡村倫彦 kn-aut-sei=岡村 kn-aut-mei=倫彦 aut-affil-num=6 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name=石津日出雄 kn-aut-sei=石津 kn-aut-mei=日出雄 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 en-keyword=内因性急死 kn-keyword=内因性急死 en-keyword=心筋梗塞 kn-keyword=心筋梗塞 en-keyword=冠状動脈血栓症 kn-keyword=冠状動脈血栓症 END start-ver=1.4 cd-journal=joma no-vol=114 cd-vols= no-issue=3 article-no= start-page=303 end-page=308 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20030131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Two Cases of Death from Hypothermia Occurring Indoors in Early Spring in Okayama Prefecture kn-title=岡山県下で起きた早春室内での凍死例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=We encountered two cases of unnatural death occurring indoors in early spring in Okayama Prefecture. The two cases were both females aged 31yearsold and 88yearsold. Autopsies revealed death from hypothermia as the cause of death. The diagnosis of death from cold was not based solely on the characteristic findings of the dead body. After confirming that there were no other accidents and diseases that may cause death, an overall evaluation should be made considering the conditions surrounding occurrence of death from cold. We describe these procedures using these two autopsy cases of death from hypothermia. en-copyright= kn-copyright= en-aut-name=IshikawaTakaki en-aut-sei=Ishikawa en-aut-mei=Takaki kn-aut-name=石川隆紀 kn-aut-sei=石川 kn-aut-mei=隆紀 aut-affil-num=1 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=2 ORCID= en-aut-name=DoiYusuke en-aut-sei=Doi en-aut-mei=Yusuke kn-aut-name=土井裕輔 kn-aut-sei=土井 kn-aut-mei=裕輔 aut-affil-num=3 ORCID= en-aut-name=TakataTomoyo en-aut-sei=Takata en-aut-mei=Tomoyo kn-aut-name=高田智世 kn-aut-sei=高田 kn-aut-mei=智世 aut-affil-num=4 ORCID= en-aut-name=ImabayashiKiyomi en-aut-sei=Imabayashi en-aut-mei=Kiyomi kn-aut-name=今林貴代美 kn-aut-sei=今林 kn-aut-mei=貴代美 aut-affil-num=5 ORCID= en-aut-name=InagakiSachiyo en-aut-sei=Inagaki en-aut-mei=Sachiyo kn-aut-name=稲垣幸代 kn-aut-sei=稲垣 kn-aut-mei=幸代 aut-affil-num=6 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name=吉留敬 kn-aut-sei=吉留 kn-aut-mei=敬 aut-affil-num=7 ORCID= en-aut-name=YamamotoYuji en-aut-sei=Yamamoto en-aut-mei=Yuji kn-aut-name=山本雄二 kn-aut-sei=山本 kn-aut-mei=雄二 aut-affil-num=8 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name=石津日出雄 kn-aut-sei=石津 kn-aut-mei=日出雄 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=2 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=3 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=4 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=5 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=6 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=7 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=8 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry affil-num=9 en-affil= kn-affil=Department of Legal Medicine Okayama University Graduate School of Medicine and Dentistry en-keyword=凍死 kn-keyword=凍死 en-keyword=下垂体 kn-keyword=下垂体 en-keyword=Wischnewsky 斑 kn-keyword=Wischnewsky 斑 END start-ver=1.4 cd-journal=joma no-vol=115 cd-vols= no-issue=1 article-no= start-page=33 end-page=37 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050530 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=An autopsy case of death after trauma in an elderly subject with pulmonary squamous cell carcinoma From the viewpoint of forensic medicine in an aging society kn-title=外傷後死亡した肺扁平上皮癌を有する高齢者の1部検例－高齢化時代を迎えつつある法医学の立場から－ en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this study, we experienced a case of an elderly man with clinically undiagnosed squamous cell carcinoma of the lung, who died 8 days after a trauma. This case was autopsied because of a question over the causal association between the trauma and death. From the autopsy findings, it was judged that respiratory and metabolic abnormalities due to lung cancer were exacerbated by trauma, and that medically the cause of death should be assessed as death by disease. en-copyright= kn-copyright= en-aut-name=IshikawaTakaki en-aut-sei=Ishikawa en-aut-mei=Takaki kn-aut-name=石川隆紀 kn-aut-sei=石川 kn-aut-mei=隆紀 aut-affil-num=1 ORCID= en-aut-name=FurusatoMasakuni en-aut-sei=Furusato en-aut-mei=Masakuni kn-aut-name=古里征国 kn-aut-sei=古里 kn-aut-mei=征国 aut-affil-num=2 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name=吉留敬 kn-aut-sei=吉留 kn-aut-mei=敬 aut-affil-num=3 ORCID= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=4 ORCID= en-aut-name=IshizuHideo en-aut-sei=Ishizu en-aut-mei=Hideo kn-aut-name=石津日出雄 kn-aut-sei=石津 kn-aut-mei=日出雄 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=2 en-affil= kn-affil=杏林大学医学部病理学教室 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯学総合研究科法医学分野 en-keyword=高齢化 kn-keyword=高齢化 en-keyword=肺癌 kn-keyword=肺癌 en-keyword=法医学 kn-keyword=法医学 END start-ver=1.4 cd-journal=joma no-vol=120 cd-vols= no-issue=1 article-no= start-page=43 end-page=48 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080501 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Immunoassays in forensic biology kn-title=法医生物学におけるイムノアッセイの応用 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MiyaishiSatoru en-aut-sei=Miyaishi en-aut-mei=Satoru kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　法医学 en-keyword=イムノアッセイ kn-keyword=イムノアッセイ en-keyword=法医学 kn-keyword=法医学 en-keyword=法医生物学 kn-keyword=法医生物学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=19910328 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒトミオグロビンの酵素免疫測定法とその法医学的応用 kn-title=AN ENZYME IMMUNOASSAY FOR HUMAN MYOGLOBIN AND ITS APPLICATION TO FORENSIC MEDICINE en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=宮石智 kn-aut-sei=宮石 kn-aut-mei=智 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END